CN109169653A - A kind of cation composite disinfectant and its application - Google Patents
A kind of cation composite disinfectant and its application Download PDFInfo
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- CN109169653A CN109169653A CN201811243879.7A CN201811243879A CN109169653A CN 109169653 A CN109169653 A CN 109169653A CN 201811243879 A CN201811243879 A CN 201811243879A CN 109169653 A CN109169653 A CN 109169653A
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- disinfectant
- guanidine
- quaternary ammonium
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/02—Amines; Quaternary ammonium compounds
- A01N33/12—Quaternary ammonium compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/30—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests characterised by the surfactants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
- A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
- A01N47/44—Guanidine; Derivatives thereof
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- General Health & Medical Sciences (AREA)
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- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Wood Science & Technology (AREA)
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- Toxicology (AREA)
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Abstract
Bacterial spore can be killed the present invention provides one kind and can be used for cationic composite disinfectant and its application of space disinfection sterilizing.The composite disinfectant is by weight, comprising: (1) 0.05-5 parts of quaternary ammonium disinfectants；(2) 0.05-5 parts of alkyl guanidine disinfectants；(3) 0.05-5 parts of amino acid type surfactants；(4) balance solvent；Composite disinfectant of the present invention satisfactorily solves the problems, such as that single quaternary ammonium disinfectant or alkyl polyguanidine class disinfectant cannot kill bacterial spore because activity is low and not be suitable for space sterilizing.Simultaneously because used quaternary ammonium salt and alkyl polyguanidine class disinfectant property are mild, both non-oxidative did not had corrosivity to the object agent surface of disinfection sterilized space class without reproducibility yet.Can be widely applied to it is various can confined space (such as hospital operating room, manufacturing enterprise workshop and isolator, laboratory and Biohazard Safety Equipment) and body surface disinfection or sterilizing.
Description
Technical field
The present invention relates to disinfection technology fields, and in particular to one kind is by quaternary ammonium salt disinfectant and guanidine disinfectant and amino acid
The composite disinfectant of type surfactant composition and its application.
Background technique
Disinfectant (disinfectant) refers to for killing pathogenic microorganism on communication media, makes up to innoxious want
The chemicals asked.Sporicide (sporicide) is then the disinfectant that gemma, spore can be killed.It is currently used to kill spore
Predominantly peroxide sporicide (such as hydrogen peroxide and Peracetic acid), Active Chlorine sporicide is (such as sub- agent category point
Hypochlorous acid/sodium and chlorine dioxide etc.) and aldehyde material sporicide (such as formaldehyde, glutaraldehyde and o-phthalaldehyde), it is used for
The method of common atomization or vaporization when space disinfection sterilizes.
Existing sporicide a part has the oxidisability such as sporicides such as hydrogen peroxide, Peracetic acid and Active Chlorine,
Corrosivity is than more serious；Another part then has reproducibility such as formaldehyde, glutaraldehyde and o-phthalaldehyde etc., generates to environment serious
Pollution and toxicity are too big, thus limit its use scope.
Quaternary ammonium disinfectant has obtained the concern and research of last 100 years as a kind of mild cationic disinfectant.Its
The aobvious neutral or alkalescent of aqueous solution, in pH 7-8.5, bactericidal effect is best；The mechanism of action is easily inhaled due to belonging to cation
Negatively charged bacterium surface is invested, changes bacterial cytoplasm permeability of the membrane, ruptures thallus, endochylema extravasation, to rise
To killing effect.At present except skin and mucous membrane disinfection, surgical hand disinfection and the medical apparatus sterilizing for hospital, it is also used for various
The disinfection of public place and all kinds of production apparatus and equipment vessel, but its antimicrobial spectrum is narrow, is easy to produce drug resistance, is used alone
Shi Buneng kills bacterial spore, thus is not suitable for space sterilizing.
Guanidine disinfectant is a kind of compound with disinfection, and the general formula of this kind of compound is: R1R2NC (=NR3)
NR4R5.Wherein, R1、R2、R3、R4、R5Represent H, alkyl, aryl.Guanidine disinfectant can be divided into alkyls for substituent group,
Aryl class.Especially molecular formula such as H2NC (=NH) NR1R2Alkyl guanidine derivatives (R1For H, alkyl, R2Represent H, alkyl, C (=
NH)NH2、CONH2、C(S)NH2Deng) it is even more good fungicide.Form of the alkyl guanidine disinfectant due to being made into salt,
So soluble easily in water, keep its easy to use, simultaneously because its antibacterial wide spectrum, low toxicity, bactericidal range is more and more extensive, except being used for
Outside surface sterilization, food and the alcoholic beverage industry of hospital sterilize, it is also used to the sterilization such as fibre paper, daily necessities such as hair
The disinfection of towel, sweater, mask etc..
For the number in guanidine compound containing guanidine group, single guanidine and biguanides and polyguanidine class can be classified as
Disinfectant etc..Single guanidine disinfectant refers to the compound containing only a guanidine group, and such as: dodecyl guanidine acetate, trade name are more
Fruit is fixed, has shown that its sterilizing ability is very strong by health organization research, is mainly used for crop bactericide；Cyanamide, from most
After being just used as pesticide, application is also more and more extensive, is now chiefly used in disinfectant, Treatment of Industrial Water, women and children's articles, food packet
Disinfection and sterilization processing of dress product etc..Currently used as mainly having for biguanides disinfectant: aryl biguanides Chlorhexidine, polyethylene are double
Guanidine hydrochloride and chlorohexidene etc..The fungicide being conventionally used as in mouthwash.It is also widely used in surface sterilization and the food of hospital
Product and alcoholic beverage industry, not only to the gram positive bacterias such as the gram positive bacterias such as staphylococcus glucose bacterium and Escherichia coli and mould
There is good inhibiting effect with good inhibiting effect, and to viral especially hepatitis B.
Polyguanidine class disinfectant is the oligomer or its salt containing one or two guanidine group numbers in molecular structure monomer,
Such as polyhexamethylene list guanidine (cas:57028-96-3), poly- [2- (2- ethyoxyl)-ethoxy ethyl ester] single guanidine (cas:374572-
91-5), poly hexamethylene biguanide and polyamidopropyl ether etc..Due to containing polymer architecture in its molecule, being allowed to toxicity is reduced,
And bactericidal effect is more excellent, has been widely used in each neck such as health care, family life, food industry and livestock-raising
Domain.
Guanidine disinfectant is similar with quaternary ammonium disinfectant, belongs to the inefficient cationic disinfectant of two classes, when exclusive use simultaneously
The effect for not killing bacterial spore may be only used for sterilizing, and reaches the general requirement of disinfectant, is not suitable for sterilizing.But
These two types of disinfectants all have toxicity it is low, to it is mucocutaneous it is non-stimulated, corrosion-free to metal fabric, by organic substance influence slightly with
And stability it is good the advantages that.Existing literature is reported when these two types of disinfectants are used in compounding, and has certain synergistic effect [Zhao
The such as Liu Chen several cation surfactant one-component and compound system help research [J] the printing and dyeing of pathogenic bacteria bactericidal property
Agent, 2017,34 (5): 24-29.], but the document does not prove whether there is killing effect to bacterial spore.
Amino acid type surfactant all has to the highly-safe of environmental and biological materials, to various other surfactants
The advantages that compatibility is good, especially cationic and amphoteric amino acids surfactant also have good bacteriostasis property and kill
Bacterium performance.It is a kind of cationic ammonia by the lauroyl arginine ethyl ester hydrochloride (LAE) that arginine and lauroyl are raw material preparation
Base acid surfaces activating agent, to gram-positive bacteria, Gram-negative bacteria, pathogen, yeast and mould all have good antibacterial
Effect can use in meat product and birds food, not have any toxic action to human body.It is anti-by N- alkylamine and monoxone
The N- alkyl glycine system amphoteric surfactant that should be prepared, which eliminates dirty (Tego) class amphoteric surfactant and one kind, to be had
The amino acid type surfactant of stronger sterilizing ability has good bactericidal properties, foaminess and wetability, to thermal stability
Good, toxicity is low.Representative products such as Tego51 (C8-18Alkylethylene diamines and chloroacetic reaction product), Tego103S (C8-18Alkyl
Diethylenetriamine is with chloroacetic reaction product) and 30 (C of Tego2000 and Rewocid WK10-16Alkyl propane diamine with it is chloroacetic
Reaction product, CAS No.139734-65-9) etc..Dirty class disinfectant is eliminated to pyococcus, enteric bacilli etc. to fungi and virus
There is good killing effect, but to bacterial spore without killing effect.Existing literature, which reports, eliminates dirty class amophoteric surface active
When agent (Tego103S) and chlorine benzalkonium chloride are used in compounding, it may have certain synergistic effect [.Tego103S such as Qu Rongjun and 1227
Synergic remove study [J] daily chemical industry, 1997,3:50-52.], but the document does not prove whether to thin
Whether bacterium gemma has killing effect.
Pct international patent (WO2010/097639 EN 2010.09.02) discloses a kind of a kind of " including biguanide compound
Antiseptic composition ", the composition can be used for killing the bacterial endospore from a kind of material, including one or more double
Guanidine polymer, one or more other active agents selected from mono-quaternaries and one or more selected from one or more
It is active to kill endospore for the other active agent of isothiazolone compounds, and the composition further comprises a kind of poly-
The blend of organosiloxane or a variety of polysiloxane, but there is no prove to can be used for space sterilizing for the composition.
Pct international patent (WO2010/000744 EN 2010.01.07) discloses one kind by fatty acid and basic amine group
The cationic surfactant that acid esters is condensed to yield, especially arginic lauramide ethyl ester (LAE) kill spore with another
The combination of sub- sodium tripolyphosphate and polysorbate can be used for handling the object for having infected spore.The technology is not also demonstrate,proved equally
It is bright whether to can be used for space sterilizing.
Summary of the invention
In order to solve existing thimerosal (sporicide) when for space disinfection sterilizing existing safety, validity,
The problem of corrosivity and stability, the present invention provides a kind of stabilizations, efficient, non-corrosive and have to bacterial spore and very well kill
Go out effect cationic composite disinfectant (sporicide) and its space disinfection sterilizing in application.
Cation composite disinfectant of the present invention includes by weight:
(1) 0.05-5 parts of quaternary ammonium disinfectants；
(2) 0.05-5 parts of alkyl guanidine disinfectants；
(3) 0.05-5 parts of amino acid type surfactants.
Sporicide of the present invention satisfactorily solves existing sporicide, and there are corrosivity, unstability and toxicity
Problem.Can be widely applied to various body surfaces and can confined space (such as hospital operating room, manufacturing enterprise workshop and isolator,
Laboratory and Biohazard Safety Equipment etc.) disinfection or sterilizing.
Preferably, the quaternary ammonium disinfectant is selected from C8-C18The list of aliphatic chain and methyl, benzyl or Ethylbenzyl composition
The mixture that one or more of chain or double-chain quaternary ammonium salt and Hydroxyethyl Ammonium Chloride pine are peaceful.
Preferably, the quaternary ammonium disinfectant is selected from single-stranded dodecyl dimethyl hexadecyldimethyl benzyl ammonium and the double decyl dimethyls of double-strand
The mixture of one or more of base ammonium.
The example of quaternary ammonium disinfectant of the present invention includes, but are not limited to: dodecyl dimethyl benzyl chloride/bromination
Ammonium (benzene pricks chlorine bromine ammonium), dodecyl dimethyl benzene oxygen ethyl phosphonium bromide ammonium (Domiphen bromide), dodecyldimethylamine yl pyridines bromination
Ammonium, double decyl dimethyl chlorine bromine ammoniums (Sha Lidi), octyl decyl dimethyl chlorine bromine ammonium, chlorine bromine (double dodecyls two
Methyl) ethylene diammonium, Hydroxyethyl Ammonium Chloride pine be peaceful and their mixture.No matter the quaternary ammonium disinfectant is used alone or closes
With all having safety low-poison, Small side effects, low concentration is effective, colourless, the odorless, advantages such as irritation is low.
The example of particularly preferred quaternary ammonium disinfectant includes for single-stranded and double-strand mixture, such as dodecyl two
Methylbenzyl chlorine bromine ammonium (single-stranded, 1st generation product) and double decyl dimethyl chlorine bromine ammoniums or octyl decyl dimethyl chlorine/
Ammonium bromide (double-strand, the 3rd generation product), as the 4th generation product, belongs to lipophilic, functional complementation according to the mixture of 1:2-2:1, than
Single disinfection method agent enhances the bactericidal activity of 1-2 logarithm, extends service life, and chemical property is stablized, and corrosivity is small, by
The hardness of organic matter and water influences small；It can also prevent cell from developing drug resistance simultaneously and enhance bactericidal activity, expand sterilization model
It encloses.Such as the dodecyl benzyl dimethyl ammonium chloride (single-stranded) of 4:6 and the mixture pair of double-track suspended self-propelled conveyor (double-strand)
Staphylococcus aureus and Escherichia coli are 250ppm in concentration, act on 30 seconds, it can reach 99.99% killing rate；It is right
Bacillus subtilis black variety bud cell acts on 10min, 6250mg/L with 12500mg/L, and effect 20min killing rate is 99.86%,
But it is extremely difficult to 99.999% killing rate (gemma for killing 6 logarithms), and the double-track suspended self-propelled conveyor of one pack system kills
Go out bacillus subtilis black variety bud cell, when effective concentration is 4000mg/L, acts on 5h, killing rate is only 95%.It can be seen that several
Kind quaternary ammonium salt shares, and only can limitedly enhance its antibacterial activity, but present invention demonstrates that the quaternary ammonium salt one-component or multicomponent
Then have the function of killing gemma when sharing with the alkyl guanidine disinfectant and amino acid type surfactant.
Preferably, oligomeric guanidine of the alkyl guanidine disinfectant in molecular structure containing one or two guanidine radicals
Disinfectant is preferably selected from polyhexamethylene list guanidine, poly hexamethylene biguanide, in poly- [2- (2- ethyoxyl)-ethoxy ethyl ester] guanidine
One or more of mixtures.No matter the alkyl guanidine disinfectant is used alone or shares, and all has antibacterial wide spectrum, low
The advantages that poison, bactericidal range is wide.
Alkyl guanidine disinfectant of the present invention can individually or with the bactericide compounded use of other types.System pH is 4-
10 is lower effective, is mutually dissolved in nonionic surfactant, ethylene glycol, glycol ether, EDTA and most of strong acid.Disappear with quaternary ammonium salt
Toxic agent is compared, and smell and foam, which have, largely to be reduced, and active constituent is to thermostabilization, non-volatile.As disinfectant, using dense
Degree is generally 2g/L~45g/L.Such disinfectant has stronger bacteriostasis to bacterial propagule wide sterilization spectrum.To various materials
Material is without damage effect, and non-corrosive, low toxicity, degradable, effect on environment is small, but one-component does not kill bacterial spore substantially
The effect of going out can only inhibit its breeding.Such as the hexamethylene thimerosal containing 1800mg/L is to large intestine bar in suspension
Bacterium acts on 5min, and average killing rate is 99.95%；The thimerosal of the list guanidine hydrochloride of polyhexamethylene containing 5000mg/L is to withered grass bar
Bacterium var. niger brood cell acts on 4h, and average killing rate is 99.66%；When polyhexamethylene list guanidine and other guanidines are according to 1:4-4:1
Ratio when sharing, antibacterial activity also improves, and such as poly- [2- (2- ethyoxyl)-ethoxy ethyl ester] guanidine is to large intestine bar
The minimum inhibitory concentration value of bacterium is 8-32mg/L, when it is shared according to the ratio and polyhexamethylene list guanidine of 3:1, minimum suppression
Bacteria concentration value is 1-8mg/L, it can be seen that, what the result and several quaternary ammonium disinfectants that several guanidine disinfectants share shared
As a result similar, it only can limitedly enhance its antibacterial activity, not kill the effect of gemma.But when alkyl guanidine of the present invention disappears
Toxic agent and quaternary ammonium salt and amino acid type surfactant then have the function of killing gemma when sharing.
Preferably, the amino acid type surfactant is selected from cationic amino acids surfactant, such as by smart ammonia
Acid is the lauroyl arginine ethyl ester hydrochloride (LAE) of raw material preparation with lauroyl, research shows that LAE is to gram-positive bacteria,
Gram-negative bacteria, pathogen, yeast and mould all have good fungistatic effect, can make in meat product and birds food
With there is no any toxic action to human body.
Preferably, the amino acid type surfactant is prepared also selected from by N- alkylamine derivative and chloroacetate reaction
N- alkyl glycine system's amphoteric surfactant eliminate dirty (Tego) class amphoteric surfactant, such as by C8-18Alkyl second two
Amine and chloroacetic reaction product (trade name Tego51), by C8-18Alkyl Diethylenetriamine and chloroacetic reaction product (commodity
Name Tego103) and by C10-16Alkyl propane diamine and chloroacetic reaction product (trade name Tego2000 and Rewocid WK
30, CAS No.139734-65-9), eliminating dirty (Tego) class amphoteric surfactant is also a kind of ammonia with stronger sterilizing ability
Base acid type surfactant has good bactericidal properties, foaminess and wetability, and good to thermal stability, toxicity is low.To suppuration ball
Bacterium, enteric bacilli etc. have good killing effect to fungi and virus, but to bacterial spore without killing effect.Existing literature
It reports and eliminates dirty class amphoteric surfactant (Tego103) and when chlorine benzalkonium chloride is used in compounding, it may have certain synergistic effect.
The specific example of amino acid type surfactant of the present invention includes, but are not limited to: LAE, Tego103,
Tego51, Tego2000 and Rewocid WK30 (CAS No.139734-65-9).Amino acid pattern surface-active of the present invention
The preferred example of agent is Tego103S, Tego2000 and LAE.
Faster sterilization effect is generated in order to make solvent used in thimerosal of the invention rapidly volatilize, this hair
It is bright other than preferred purified water is as solvent, it is also an option that water and other volatility C2-C3The mixed solution of alcohol composition is as molten
Agent；The C2-C3Alcohol is selected from the mixture of one or more of ethyl alcohol, normal propyl alcohol and isopropanol, as long as can using concentration
Dissolution each constituent, preferably accounts for the range of the solvent 0.1%-99% (w/w), more preferable 1%-50%
(w/w) range.
Composite disinfectant of the invention can also contain optionally other other than comprising main constituents described above
The substance that can be dissolved each other, such as stabilizer, surfactant, corrosion inhibitor, wetting agent, aromatic and colorant etc..These are added
Intersolubility substance be known in the art and chemical reaction or incompatibility do not occur with already existing other ingredients.Its
Additional amount is then different and different according to the type that substance is added, as long as required or defined residual after not increasing sterilization
Level does not reduce its sterilization effect, and the present invention does not limit this particularly.
As a preferred embodiment of the invention, cation composite disinfectant of the present invention includes following weight
The component of percentage:
(1) 0.1-1% quaternary ammonium disinfectant
(2) 0.1-1% alkyl guanidine disinfectant
(3) 0.1-1% amino acid type surfactant
(4) balance solvent；
The quaternary ammonium disinfectant is in dodecyl benzyl dimethyl ammonium chloride and/or double-track suspended self-propelled conveyor
One or more of mixtures；The alkyl guanidine disinfectant is polyhexamethylene (single, double) guanidine and/or poly- [2- (2- second
Oxygroup)-ethoxy ethyl ester] one or more of guanidine mixture；The amino acid type surfactant be LAE,
The mixture of one or more of Tego2000 or Tego103S；The solvent is water or water and C2-C3The mixing of alcohol composition is molten
Liquid.
As another preferred embodiment of the present invention, cation composite disinfectant of the present invention includes following heavy
Measure the component of percentage:
(1) 0.1-0.5% quaternary ammonium disinfectant, the quaternary ammonium disinfectant is benzalkonium chloride or weight ratio is 1:4-
The benzalkonium chloride of 4:1 and the mixture of double-track suspended self-propelled conveyor；
(2) 0.1-0.5% alkyl guanidine disinfectant, the alkyl guanidine disinfectant be polyhexamethylene (single, double) guanidine or
Weight ratio is polyhexamethylene (single, double) guanidine of 1:4-4:1 and the mixture of poly- [2- (2- ethyoxyl)-ethoxy ethyl ester] guanidine；
(3) 0.1-0.5% amino acid type surfactant, the amino acid type surfactant is LAE or weight ratio is
The mixture of the Tego2000 and LAE of 1:4-4:1；
(4) surplus is the mixed solution of water or ethyl alcohol and water.
Cation composite disinfectant of the present invention can be made by this field customary preparation methods, and final products are
Bright solution.It must not be also not suitable for and iodine or peroxide (such as permanganic acid with soap or other anionic detergents with using when use
Potassium, hydrogen peroxide, sulfa powder etc.) it shares.
Invention also provides the cationic composite disinfectants in surface disinfection and space disinfection or sterilizing
Application.
The present invention passes through by quaternary ammonium disinfectant, alkyl guanidine disinfectant and with the amino acid of disinfecting and sterilizing functions
Type surfactant is compounded, and the alcohol (C of water or water and low carbon chain is selected1-C3) composition mixed solution as solvent preparation
At composite disinfectant (sporicide), there is very strong sterilization effect compared with the other disinfectant of unitary class, especially to bacterium
Gemma has very strong killing effect (without killing gemma when wherein the disinfectant of single classification or two of them classification compounds
Effect).Since cationic composite disinfectant (sporicide) prepared by the present invention the other disinfectant of 3 types although be made of,
But oxidizing substance such as hydrogen peroxide, Peracetic acid and Active Chlorine etc. are not contained, without corrosivity；Reducing substances are not contained yet
Such as formaldehyde, glutaraldehyde and o-phthalaldehyde will not generate serious pollution to environment, also without apparent toxic side effect, therefore make
To be preferred, which is particularly suitable for using spraying method, for various body surfaces and
Can confined space (such as hospital operating room, manufacturing enterprise workshop and isolator, laboratory and Biohazard Safety Equipment) disinfection or go out
Bacterium.
Compared with sporicide product currently on the market, the invention has the characteristics that:
(1) a large amount of strong electrolytes and oxidizing substance are free of, because being particularly suitable for using spray without apparent corrosiveness
The method of mist, for various body surfaces and can confined space (such as hospital operating room, manufacturing enterprise workshop and isolator, experiment
Room and Biohazard Safety Equipment etc.) disinfection or sterilizing；
(2) due to a few class disinfectants of addition have synergetic antibacterial effect, improve its sterilization activity, reduction it is resistance to
Pharmacological property.
Specific embodiment
Various raw materials used in the present invention are known commercial product, as dodecyl benzyl dimethyl ammonium chloride can
It is purchased from Guangzhou Du Te Chemical Co., Ltd., double-track suspended self-propelled conveyor can be purchased from Jin Ao Chemical Co., Ltd. of Anhui Province；It is poly-
Hexa-methylene list guanidine and poly hexamethylene biguanide can be purchased from Shanghai Gaoju Biotechnology Co., Ltd.；LAE can be purchased from Shaanxi Cheng Rui
Biotechnology Co., Ltd, Tego2000 or Tego103S can be purchased from Guangzhou Tuo Xin Chemical Industry Science Co., Ltd.
Embodiment 1-6 in following table 1 is not intended to limit the scope of the invention for illustrating the present invention.Its performance is same
Reference examples 1-6 in Shi Yubiao 2 is compared.The concentration of each component refers to mass percent concentration.
Each composition and ratio of embodiment 1-6 that table 1 is made of 3 substances
Each composition and ratio of reference examples 1-6 that table 2 is made of 2 substances
Performance test
1. corrosivity is tested
Referring to disinfection technology standard (2008), using carbon steel, stainless steel, copper and aluminium as subjects, the present invention is tested
Composite disinfectant (embodiment 1 and embodiment 2) corrosivity (be diluted to 0.5% concentration before test with purified water and have
Imitate ingredient), while being compared with the reference examples 1 of the polyhexamethylene guanide of same concentrations (PHMG) and same concentrations, specific side
Method is as follows:
Take carbon steel, stainless steel, copper and aluminium respectively, be made diameter be 24 ± 0.2mm, thickness about 1.0mm,
And have the disk of an about 2.0mm diameter aperture, when test, surface oxide layer is ground off, after actual measurement diameter, thickness and hole
After diameter, its surface area is calculated, then clean and in 50 DEG C of drying 1h.After weighing its quality, it is soaked in experimental solutions (by 20ml/
cm2Fluid volume), when immersion, a kind of metal, 1 container replaces 1 experimental solutions for 24 hours.It is dipped to 72h, takes out sheet metal, with
Distilled water flushing is clean, through 50 DEG C of drying 1h.Metal tablet quality after impregnating is weighed, calculates corrosion rate R according to its mass loss
(mm/a)。
[R is corrosion rate, mm/a (millimeter/year)；M is metal sheet weight before testing, g；mtFor metal slice weight after test
Amount, g；mkRemove corrosion product print weightless value for chemical treatment, g does not carry out chemical scavenging processor in test, when calculating
Leave out m in formulakValue；S is the surface area total value (cm of sheet metal2)；T is test period, h；D is metal material density, kg/
m3。]
Test result is shown in Table 3.As shown in Table 3, composite disinfectant of the invention (sporicide) is to corrosion of metal
Property (reference examples 1) consistent with one-component or bi-component thimerosal, corrosivity are lower.
3 corrosivity test result of table
2. space sterile test and surface sterilization test
(1) space sterile test method and result
It takes and carries out space sterile test by the cationic composite disinfectant that 1 embodiment 1-6 of table is prepared, and simultaneously and by table 1
The thimerosal of reference examples 1-6 configuration compares, and the specific method is as follows.
1. main experimental materials
Hot air sterilization biological indicator: mark bacterium piece bacteria containing amount is not less than 1.0 × 106CFU/ piece, by hay bacillus
(ATCC9372) Bacillus piece and phenol red culture solution instruction pipe two parts composition, it is after cultivation, residual if sterilization treatment is incomplete
The gemma growth deposited, producing acid reduces pH, and culture solution color is turned yellow by red, if sterilization treatment is complete, culture solution color
(Nanjing Bian Zhen Biotechnology Co., Ltd) is remained unchanged,- 1 type low-temperature vaporization nanometer fog generator (Wuhan City's nanometer mist
Science and Technology Ltd. provides, and atomization speed 15ml/min can be by disinfectant liquid atomization to 1 micron of little particulate below).
2. test space
Certain pharmacy corporation workshop, high 2.8m, wide 4.4m, long 6.5m, space total volume about 80m3。
3. test method
Nanometer fog generator is placed among workshop, 4, workshop metope and 4 corner arrangement spore amounts are 106CFU/
The biological indicator of piece totally 8 (number is respectively 1,2,3,4,5,6,7,8), the height of the biological indicator of arrangement from the ground is
1m, adds the sporicide or solution of test, closed all door and windows, when standing different after shutting down after booting atomization 80min
Between (be approximately equivalent to atomization 15ml/m3), biological indicator (gemma item) is taken out by aseptic procedure, is put into the gemma training of reference numeral
In nutrient solution, according to the operation instructions of purchased biological indicator, by the culture solution containing bacillus subtilis bacterium piece be placed in 30 DEG C-
It is observed after cultivating 48h in 37 DEG C of constant incubator, while taking same batch biological indicator 1 to make positive control, if culture 48h
The color of culture solution becomes yellow from red afterwards, that is, indicates bacterium growth, determines that sterilizing is unqualified；As still indicated sterile to be red
Growth, while positive control growth is normal, is determined as the qualification that sterilizes.
4. experimental result
Experimental result is shown in Table shown in 4.As shown in Table 4, when atomization volume is 15mL/m3, seal time is greater than 60min
When, the composite disinfectant prepared by 1 embodiment 1-6 of table is the ability with killing gemma, and by the preparation of table 2 by 2 substances
Ability of the reference examples 1-6 of composition without killing gemma.Thus illustrate that composite disinfectant of the invention is a kind of sporicide,
It can be used for the disinfection and sterilizing in space.
The different embodiments of table 4 and reference examples are to space sterile test result
Note: constant yellow after indicator culture indicates asepsis growth, labeled as-；Become yellow after instruction culture, indicates
There is bacterium growth, labeled as+；0/8 indicates equal asepsis growth in 8 biological indicators；8/8 indicates in 8 biological indicators by 8
There is bacterium growth；Remaining and so on.
(2) surface sterilization test method and result
1. surface sterilization test method
It takes and carries out surface sterilization test by the composite disinfectant (sporicide) that 1 embodiment 1 of table is prepared: using the side of atomization
Method, the sporicide prepared with embodiment 1 is to 80m3Space carry out nanometer mist space disinfection, sprayed volume 15ml/m3, close
Closing the time is 120min.After sanitized, using (4 metopes each 2, each surface in clean area after aseptic cotton carrier wiping sterilizing
8 points are selected on point, ground at random), the erasing area of each point is 5*5cm2, after having wiped, with aseptic operation scissors cotton swab
Head (cotton position) is cut, and investment is equipped in the conical flask of 50ml0.9% aseptic sodium chloride solution, closed.Sample will be housed
Conical flask shake repeatedly, make the Microbiological release on cotton swab into solution.Sample solution 50ml membrane filtration is taken, filter is taken out
Film, bacterium is face-up, is affixed on TSA culture medium, 30-35 degree culture 5 days, counts and compares with the testing result before sterilizing.
2. surface sterilization test result
It the results are shown in Table shown in 5, as shown in Table 5: when atomization volume is 15ml/m3, when seal time is 120 minutes, this hair
Bright composite disinfectant (sporicide) cannot be only used for space sterilizing, it may also be used for and body surface carries out disinfection, after disinfection, wall
The surface microorganism of wall, ground and equipment meets the requirement of GMP environment.
The comparison of the sterilizing front and rear surfaces microbial contamination level of table 5
In conclusion according to cationic composite disinfectant of the present invention, disinfection and sterilization ability is better than one-component
Thimerosal, be also better than the thimerosal of bi-component, but also there is the ability for killing bacterial spore.Composite disinfecting of the present invention
Liquid satisfactorily solve single quaternary ammonium disinfectant or alkyl polyguanidine class disinfectant because activity it is low cannot kill bacterial spore and
Not the problem of not being suitable for space sterilizing.Simultaneously because used quaternary ammonium salt and alkyl polyguanidine class disinfectant property are mild,
Both non-oxidative does not have corrosivity to the object agent surface of disinfection sterilized space class without reproducibility yet.It can be widely applied to various
It can confined space (such as hospital operating room, manufacturing enterprise workshop and isolator, laboratory and Biohazard Safety Equipment) and body surface
Disinfection or sterilizing.
As described above, the present invention is only based on claims, by several specific embodiments and embodiment into
Row is explained, but the present invention is not limited to this.The content recorded in detail in the claims can implement numerous embodiments, any
Those skilled in the art it is conceivable that various change, should all fall in the protection domain of the application.
Claims (10)
1. a kind of cation composite disinfectant, which is characterized in that by weight, include:
(1) 0.05-5 parts of quaternary ammonium disinfectants；
(2) 0.05-5 parts of alkyl guanidine disinfectants；
(3) 0.05-5 parts of amino acid type surfactants.
2. cation composite disinfectant according to claim 1, which is characterized in that the quaternary ammonium disinfectant is selected from C8-
C18Aliphatic chain and methyl, the single-stranded or double-stranded quaternary ammonium salt of benzyl or Ethylbenzyl composition and Hydroxyethyl Ammonium Chloride pine it is one of peaceful or
Several mixtures.
3. cation composite disinfectant according to claim 1 or 2, which is characterized in that the quaternary ammonium disinfectant choosing
From single-stranded dodecyl dimethyl hexadecyldimethyl benzyl ammonium and/or the double dimethylammoniums of double-strand.
4. cation composite disinfectant according to any one of claim 1-3, which is characterized in that the alkyl guanidine disappears
Oligomeric guanidine disinfectant of the toxic agent in molecular structure containing one or two guanidine radicals；
It is preferred that the alkyl guanidine disinfectant is selected from polyhexamethylene list guanidine, poly hexamethylene biguanide, poly- [2- (2- ethyoxyl)-
Ethoxy ethyl ester] one or more of guanidine mixture.
5. cationic composite disinfectant described in any one of -4 according to claim 1, which is characterized in that the amino acid pattern table
Face activating agent is selected from cationic amino acids surfactant and/or N- alkyl glycine system amphoteric amino acids surfactant.
6. cation composite disinfectant according to any one of claims 1-5, which is characterized in that the composite disinfectant
It further include solvent；
The solvent is water or water and C2-C3The mixed solution of alcohol composition；
Preferably, the C2-C3Alcohol is selected from one or more of ethyl alcohol, normal propyl alcohol and isopropanol mixture.
7. cation composite disinfectant according to claim 1, which is characterized in that the composite disinfectant includes following heavy
Measure the component of percentage:
(1) 0.1-1% quaternary ammonium disinfectant；
(2) 0.1-1% alkyl guanidine disinfectant；
(3) 0.1-1% amino acid type surfactant；
(4) balance solvent；
The quaternary ammonium disinfectant is one in dodecyl benzyl dimethyl ammonium chloride and/or double-track suspended self-propelled conveyor
Kind or several mixtures；The alkyl guanidine disinfectant is polyhexamethylene (single, double) guanidine and/or poly- [2- (2- ethoxy
Base)-ethoxy ethyl ester] one or more of guanidine mixture；The amino acid type surfactant is LAE, Tego2000
Or the mixture of one or more of Tego103S；The solvent is water or water and C2-C3The mixed solution of alcohol composition.
8. it is according to claim 1 or claim 7 cation composite disinfectant, which is characterized in that the composite disinfectant include with
The component of lower weight percent:
(1) 0.1-0.5% quaternary ammonium disinfectant, the quaternary ammonium disinfectant is benzalkonium chloride or weight ratio is 1:4-4:1
Benzalkonium chloride and double-track suspended self-propelled conveyor mixture；
(2) 0.1-0.5% alkyl guanidine disinfectant, the alkyl guanidine disinfectant is polyhexamethylene guanide or weight ratio is 1:4-
The mixture of the polyhexamethylene guanide of 4:1 and poly- [2- (2- ethyoxyl)-ethoxy ethyl ester] guanidine；
(3) 0.1-0.5% amino acid type surfactant, the amino acid type surfactant is LAE or weight ratio is 1:4-
The mixture of the Tego2000 and LAE of 4:1；
(4) surplus is 1-50% ethanol water.
9. application of the cation composite disinfectant of any of claims 1-8 in space disinfection or sterilizing.
10. application of the cation composite disinfectant of any of claims 1-8 in surface disinfection or sterilizing.
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| CN109777639A (en) * | 2019-02-22 | 2019-05-21 | 江苏今日卫生用品有限公司 | It is a kind of for air-conditioning and the disinfection of general object hard surface and the preparation of hygiene and preparation method thereof |
| CN109997849A (en) * | 2019-04-28 | 2019-07-12 | 河北瑞鸿生物科技有限公司 | A kind of thimerosal and preparation method thereof |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1185480A (en) * | 1996-12-20 | 1998-06-24 | 花王株式会社 | Detergent composition |
| CN102065851A (en) * | 2008-04-25 | 2011-05-18 | 纳米生物公司 | Nanoemulsions for treating fungal, yeast and mold infections |
| CN104135858A (en) * | 2011-12-29 | 2014-11-05 | 拜奥特罗尔有限公司 | Anti-microbial composition |
| CN107466207A (en) * | 2015-04-02 | 2017-12-12 | 拜奥特罗尔有限公司 | Antimicrobial compositions |
-
2018
- 2018-10-24 CN CN201811243879.7A patent/CN109169653B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1185480A (en) * | 1996-12-20 | 1998-06-24 | 花王株式会社 | Detergent composition |
| CN102065851A (en) * | 2008-04-25 | 2011-05-18 | 纳米生物公司 | Nanoemulsions for treating fungal, yeast and mold infections |
| CN104135858A (en) * | 2011-12-29 | 2014-11-05 | 拜奥特罗尔有限公司 | Anti-microbial composition |
| CN107466207A (en) * | 2015-04-02 | 2017-12-12 | 拜奥特罗尔有限公司 | Antimicrobial compositions |
Cited By (14)
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| CN109777639B (en) * | 2019-02-22 | 2021-11-16 | 江苏今日卫生用品有限公司 | Preparation for disinfecting and cleaning hard surface of air conditioner and general objects and preparation method thereof |
| CN109997849A (en) * | 2019-04-28 | 2019-07-12 | 河北瑞鸿生物科技有限公司 | A kind of thimerosal and preparation method thereof |
| CN111466416A (en) * | 2020-04-20 | 2020-07-31 | 重庆森盟新能源开发有限公司 | Composite disinfectant using photocatalysis technology |
| CN111449993A (en) * | 2020-04-27 | 2020-07-28 | 中国日用化学研究院有限公司 | Foam type low-carbon alcohol system hand disinfectant and preparation method thereof |
| CN111802409A (en) * | 2020-06-23 | 2020-10-23 | 武汉理工大学 | Broad-spectrum antiviral antibacterial disinfectant and preparation method and application thereof |
| CN111802409B (en) * | 2020-06-23 | 2021-10-29 | 武汉理工大学 | Broad-spectrum antiviral antibacterial disinfectant and preparation method and application thereof |
| CN111849233A (en) * | 2020-07-06 | 2020-10-30 | 中国科学院大学温州研究院(温州生物材料与工程研究所) | Super wear-resistant long-acting antibacterial and antiviral nano coating and preparation method thereof |
| CN111838171A (en) * | 2020-08-14 | 2020-10-30 | 上海黛龙生物工程科技有限公司 | Compound disinfectant and preparation method thereof |
| CN112205432A (en) * | 2020-09-27 | 2021-01-12 | 杭州氢态生物科技有限公司 | Cationic composite disinfectant based on plant antibacterial extract |
| CN114788521A (en) * | 2022-03-23 | 2022-07-26 | 广州先进技术研究所 | Composite synergistic multifunctional disinfectant and preparation method thereof |
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