CN102405807B - Method by utilizing acid electroproduction functional water to inhibit tobacco blackleg germs - Google Patents
Method by utilizing acid electroproduction functional water to inhibit tobacco blackleg germs Download PDFInfo
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- CN102405807B CN102405807B CN 201110220273 CN201110220273A CN102405807B CN 102405807 B CN102405807 B CN 102405807B CN 201110220273 CN201110220273 CN 201110220273 CN 201110220273 A CN201110220273 A CN 201110220273A CN 102405807 B CN102405807 B CN 102405807B
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Abstract
The invention relates to a method by utilizing acid electroproduction functional water to inhibit tobacco blackleg germs, which is characterized in that: acid electroproduction functional water is adequately contacted with tobacco blackleg germs, the contact time is 30s to 300s, the acid electroproduction functional water is sprayed one time every 24h to 72h, and the inhibition rate of the acid electroproduction functional water to the tobacco blackleg germs is calculated by adopting a computer image processing method; and the subacid electroproduction functional water has an inhibition effect on the tobacco blackleg terms, and the inhibition rate can reach more than 50 percent. The production method is simple, the production cost is low, the processing cost is low, and the acid electroproduction functional water utilized by the method is safe, harmless and residue-free and is free from safety potential danager.
Description
Technical field
The present invention relates to the method that a kind of acid electrolyzed functional water suppresses tobacco black shank bacterium, belong to the crop pest control technical field.
Background technology
" electrolyzed functional water (Electrolyzed Functional Water, EFW) " also claims electrolytic ionic water, electrolyzed oxidizing water and brine electrolysis etc.It is weak solution, the acid electrolyzed functional water that obtains under the processing of special electrolysis unit and the general name of alkaline electrolyzed functional water of chloride compound and drinking water or hydrochloric acid and drinking water.Wherein, acid electrolyzed functional water has lower pH value, higher redox potential (Oxidation-Reduction Potential, ORP) and certain effective chlorine density (Available Chlorine Concentration, ACC).It mainly comprises: highly acid electrolyzed functional water (pH＜3.0, ORP〉1000 mV, ACC 20-100 mg/L), subacidity electrolyzed functional water (pH 5.0-6.5, ORP 800-1000 mV, ACC 20-100 mg/L).Bactericidal effect receives much attention acid electrolyzed functional water owing to having widely, and is used in a lot of aspects.At present, the application study for acid electrolyzed functional water both at home and abroad mainly concentrates on health care, food processing, agricultural production three large fields, has obtained abundant achievement in research.Japan in 2002 improves people's living condition and classifies acid electrolyzed functional water as the food additives category.
Balck shank is a kind of systematic fungal disease in China's cured tobacco production.Black shank bacterium is facultative saprophyte, can be with mycelium, sporangium, zoospore, chlamydospore, the survival of egg spore variform structure, mainly the invalid body in soil or muck is survived the winter with resting mycelium body and chlamydospore, generally can survive about 3 years, when next year, flue-cured tobacco field-transplanting phase environmental condition was suitable, germ forms the source of dying then with amount reproduction.
Black shank is distributed widely in the World tobacco growing area, also is one of Major Diseases on China tobacco.Investigating Shandong, Henan and the Anhui in China the Yellow River and Huai He River cigarette district according to us, is in history more serious balck shank district.Yunnan, Guizhou, Guangdong, Fujian, Hunan are also more general.Also there is generation in the Liaoning in northeast.The incidence of disease of these lesiones is generally 10-15%, and severe one reaches 50%.Mainly contain for the method that suppresses black shank at present: (1) selection and popularization disease-resistant variety; (2) cultural control measure is selected anosis strong sprout, efficent rotation, early plants in good time, improves cultivation step, is noted the field health during such as cultivation; (3) chemical control, 75% thiophanate methyl, 25% metalaxyl, 95% fenaminosulf, 0.3% aliette or 58% metalaxyl MnZn, 53% Ridomil MZ Gold.But the method for above-mentioned inhibition balck shank has certain limitation, and as generally investment is large, the cycle is long, and the application scale is less, is difficult to use; And that the chemical control method can cause is necessarily residual, and to environment, therefore also there is certain shortcoming in long half time.
Summary of the invention
The object of the invention is to the defective for above-mentioned prior art existence, a kind of method of utilizing acid electrolyzed functional water to suppress tobacco black shank bacterium is provided.The method is simple, and processing cost is low, and noresidue can obviously suppress tobacco black shank bacterium, is a kind of novel practical, method safely and effectively.
A kind of method of utilizing acid electrolyzed functional water to suppress tobacco black shank bacterium, concrete scheme is as follows:
Spray tobacco black shank bacterium with acid electrolyzed functional water, when spraying, be 30-300 s the time of contact of acid electrolyzed functional water and black shank at every turn, sprays an acid electrolyzed functional water every 24-72 h; Described acid electrolyzed functional water comprises highly acid electrolyzed functional water (AEFW) and subacidity electrolyzed functional water (SEFW); The pH value of described highly acid electrolyzed functional water is not higher than 3.0, and redox potential is 1000-1200 mV, and effective chlorine density is 20-100 mg/L; The pH value of described subacidity electrolyzed functional water is 5.0-6.5, and redox potential is 800-1000 mV, and effective chlorine density is 20-100 mg/L.Preferred 60-100 mg/L of the effective chlorine density of described acid electrolyzed functional water.
The preferred subacidity electrolyzed functional water of described acid electrolyzed functional water.
The time interval that described acid electrolyzed functional water sprays is preferred 48 h, namely sprays an acid electrolyzed functional water every 48 h.
Described acid electrolyzed functional water needs fully to contact with tobacco black shank bacterium.
The time of contact of described acid electrolyzed functional water and tobacco black shank bacterium preferred 30 s.
Compared with prior art, the advantage that the present invention has and beneficial effect are: it is remarkable that (1) the present invention suppresses the tobacco black shank bacterium effect, and inhibiting rate is up to more than 50%; (2) production method of the present invention is simple, production cost is low, and the present invention only need bear water rate and the electricity charge, so cost is very low; (3) the used acid electrolyzed functional water of the present invention is safe, harmless, can be reduced to light water after use, can not work the mischief to environment, and safety concerning the user does not have nocuity and residual equally.The method is a kind of novel practical, method safely and effectively.
Another purpose of the present invention has provided and has adopted the Computer Image Processing method to calculate acid electrolyzed functional water to the method for tobacco black shank bacterium inhibiting rate.
Fig. 2 has shown the bacterium colony of black shank bacterium after cultivating a period of time, calculates the interior area of first circle by the method for Computer Image Processing
S 1, second circle inner area
S 2, the 3rd circle inner area
S 3Spray for the first time the amplification rate of tobacco black shank bacterium behind the acid electrolyzed functional water:
In the formula
: the amplification rate of spraying for the first time tobacco black shank bacterium behind the acid electrolyzed functional water
S 2-1: the area between first circle and second circle
Spray for the second time the amplification rate of tobacco black shank bacterium behind the acid electrolyzed functional water:
In the formula
: the amplification rate of spraying for the second time tobacco black shank bacterium behind the acid electrolyzed functional water
S 3-2: the area between second circle and the 3rd circle
Calculating the amplification rate of black shank bacterium can eliminate with the method
S 1,
S 2The random error of bringing.
Acid electrolyzed functional water is to the inhibiting rate of tobacco black shank bacterium:
In the formula
: acid electrolyzed functional water is to the inhibiting rate of tobacco black shank bacterium
Description of drawings
Fig. 1 sprays the amplification rate (s times of contact 300) of tobacco black shank bacterium behind the acid electrolyzed functional water of different indexs;
Wherein
XAxle is the different effective chlorine densities of acid electrolyzed functional water, and Control is that the control group effective chlorine density is 0 mg/L,
YAxle is tobacco black shank bacterium amplification rate;
Fig. 2 sprays the bacterium colony contrast (s times of contact 300) of acid electrolyzed functional water and the tobacco black shank bacterium behind the sterile water of pH6.06, effective chlorine density 91 mg/L;
Cultivate the afterwards size of black shank bacterium colony through 144 h, be followed successively by from inside to outside first circle, second circle, the 3rd circle, the area that first circle surrounds is the bacterium colony size that 48 h are cultivated in inoculation afterwards, area between first circle and second circle is to spray the bacterium colony size of cultivating 48 h behind the subacidity electrolyzed functional water of pH6.06, effective chlorine density 91 mg/L for the first time, is to spray the bacterium colony size of cultivating 48 h behind the subacidity electrolyzed functional water of pH6.06, effective chlorine density 91 mg/L for the second time between the 3rd circle and second circle;
The subacidity electrolyzed functional water of Fig. 3 pH6.06, effective chlorine density 90 mg/L and tobacco black shank bacterium time of contact are on the impact of tobacco black shank bacterium amplification rate;
Wherein
XAxle is the time that the subacidity electrolyzed functional water of pH6.06, effective chlorine density 90 mg/L contacts with tobacco black shank bacterium, and Control is that be 300 s the time of contact of black shank and sterile water,
YAxle is tobacco black shank bacterium amplification rate;
The subacidity electrolyzed functional water of Fig. 4 pH6.06, effective chlorine density 90 mg/L contacts 30 s and 5 min Contrast on effects with tobacco black shank bacterium.
Concrete embodiment
The preparation of acid electrolyzed functional water
(1) is that 3% ratio is diluted in concentrated hydrochloric acid in the distilled water according to mass percent, stirs, then place the electrolyte bottle of acid electrolyzed functional water process units;
(2) at voltage 5 V, electric current 8 A, hydrochloric acid solution flow 1.02 L/h, carry out continuously cell reaction under the condition of raw water flow 100 L/h, produce acid electrolyzed functional water; The gained acid electrolyzed functional water is the acid electrolyzed functional water of pH=2.33 ± 0.10, effective chlorine density 90 ± 3 mg/L, redox potential 1187 ± 10 mV after testing.
Embodiment 1 acid electrolyzed functional water is to the inhibition test of tobacco black shank bacterium
(1) test material
Tobacco black shank bacterium.
(2) pre-treatment of test material
At first tobacco black shank bacterium is seeded in and shifts to an earlier date on the ready potato dextrose agar flat board, being placed on temperature is that it is for subsequent use to cultivate 48 h in 28 ± 1 ℃ of constant incubators.
(3) processing of test
The acid electrolyzed functional water of preparation diluted or add the acid electrolyzed functional water that sodium bicarbonate solution is prepared following index:
Sterile water (contrast) pH=7.33 ± 0.10, ORP=174 mV, ACC=0 mg/L.
(4) test method
Ready 42 flat boards of inoculating tobacco black shank bacterium without miscellaneous bacteria are divided into 7 groups at random, go out the at this moment size of bacterium colony at each dull and stereotyped back side with delineating stroke, all put in the superclean bench of the bacterium of having gone out in advance, in superclean bench, the acid electrolyzed functional water of 7 kinds of different indexs is sprayed onto respectively 7 windings and grows tobacco in the flat board of black shank bacterium in the table 1 that will prepare before test, sprinkling amount in each flat board is 20 mL, the principle of choosing 20 mL is can flood whole tobacco black shank bacteriums for the acid electrolyzed functional water that guarantees different indexs, can play antibacterial effect because only have acid electrolyzed functional water to contact just with tobacco black shank bacterium, be 300 s time of contact, then the acid electrolyzed functional water of the different indexs in each flat board is poured in the waste liquid cylinder, burn dull and stereotyped border with alcolhol burner, prevent pollution microbes, the time interval that the constant incubator that has the inoculation of handling well the flat board of tobacco black shank bacterium to put into 28 ± 1 ℃ is at last cultivated twice sprinkling of 48 h(also is 48 h), take out, again draw the size of the bacterium colony of this moment at each dull and stereotyped back side; Repeat successively above-mentioned steps, spray again once the acid electrolyzed functional water of 7 kinds of different indexs of preparation in the table 1, take out behind 48 h, draw a circle, the amplification rate of tobacco black shank bacterium behind the different index acid electrolyzed functional waters of the each sprinkling of calculating.
(5) acid electrolyzed functional water is to the calculating of tobacco black shank bacterium inhibiting rate
(6) result of the test
Result's (seeing Fig. 1 and Fig. 2), the tobacco black shank bacterium amplification rate of spraying different index acid electrolyzed functional waters be starkly lower than control group (
p＜0.01), the effective chlorine density of subacidity electrolyzed functional water on the impact of the amplification rate of tobacco black shank bacterium extremely significantly (
p＜0.01), under same effective chlorine density, different pH on the impact of the amplification rate of tobacco black shank bacterium not significantly (
p0.05).When effective chlorine density reaches 90 mg/L, spray behind highly acid and the subacidity electrolyzed functional water amplification rate of tobacco black shank bacterium almost identical (
p0.05), inhibiting rate is up to more than 50%, because strong acid can have injury effect to plant, and test is found, when effective chlorine density reaches 90 mg/L, highly acid and subacidity electrolyzed functional water are not remarkable on the inhibiting rate impact of black shank, therefore preferred subacidity electrolyzed functional water (pH6.06, ACC=90 mg/L, ORP=922 mV).
(1) test material
Tobacco black shank bacterium.
(2) test material pre-treatment
See embodiment 1.
(3) processing of test
According to the result that embodiment 1 draws, adopt the subacidity electrolyzed functional water to test, the highly acid electrolyzed functional water of preparation is added the subacidity electrolyzed functional water that sodium bicarbonate solution is prepared following index:
Subacidity electrolyzed functional water: pH=6.06, redox potential ORP=922 mV, effective chlorine density ACC=90 mg/L;
Sterile water (contrast) pH=7.33 ± 0.10, ORP=174 mV, ACC=0 mg/L.
(4) test method
Ready 30 aseptic flat boards of inoculating tobacco black shank bacterium are divided into 5 groups at random, go out the at this moment size of bacterium colony at each dull and stereotyped back side with delineating stroke, all put in the superclean bench of the bacterium of having gone out in advance, in superclean bench, the subacidity electrolyzed functional water that will prepare before test is sprayed onto respectively wherein 4 windings and grows tobacco in the flat board of black shank bacterium, sprinkling amount in each flat board is 20 mL, the principle of choosing 20 mL is in order to guarantee that the subacidity electrolyzed functional water floods whole tobacco black shank bacteriums, can play antibacterial effect because only have acid electrolyzed functional water to contact just with tobacco black shank bacterium, be respectively 30 s 4 groups time of contact, 60 s, 120 s, 300 s, control group sprays 20 mL sterile waters, and be 300 s time of contact.Pour subacidity electrolyzed functional water in each flat board in waste liquid cylinder after finishing time of contact, burn dull and stereotyped border with alcolhol burner, prevent pollution microbes, the time interval that the constant incubator that has the inoculation of handling well the flat board of tobacco black shank bacterium to put into 28 ± 1 ℃ is at last cultivated twice sprinkling of 48 h(also is 48 h), take out, again draw the size of bacterium colony this moment at each dull and stereotyped back side; After repeating successively again above-mentioned steps 3 times, all to draw at the dull and stereotyped back side size of bacterium colony before each the sprinkling, calculate each amplification rate of spraying tobacco black shank bacterium behind the subacidity electrolyzed functional water.
(5) acid electrolyzed functional water is to the calculating of tobacco black shank bacterium inhibiting rate
(6) result of the test
The result shows (seeing Fig. 3 and Fig. 4), spray behind the subacidity electrolyzed functional water tobacco black shank bacterium amplification rate significantly be lower than control group (
p＜0.01), inhibiting rate is up to more than 50%, subacidity electrolyzed functional water time of contact different from black shank bacterium on the impact of the amplification rate of tobacco black shank bacterium not significantly (
p0.05).In actual applications, can adopt the mode of spraying or irrigating to realize the control of black shank, and these two kinds of methods of application can guarantee that subacidity electrolyzed functional water and germ have sufficient time of contact, but according to experimental result, time of contact is not remarkable on the bacteriostasis rate impact, therefore, subacidity electrolyzed functional water and tobacco black shank bacterium time of contact can preferred 30 s.
It should be noted that at last, above embodiment is only unrestricted in order to technical scheme to be described, although with reference to preferred embodiment the present invention is had been described in detail, those skilled in the art can make amendment or is equal to replacement technical scheme of the present invention, and not breaking away from the spirit and scope of technical solution of the present invention, it all should be encompassed in the middle of the claim scope of the present invention.
Claims (5)
1. an acid electrolyzed functional water suppresses the method for tobacco black shank bacterium, it is characterized in that: spray tobacco black shank bacterium with acid electrolyzed functional water, acid electrolyzed functional water needs to contact with tobacco black shank bacterium, during each the sprinkling, be 30-300s the time of contact of acid electrolyzed functional water and tobacco black shank bacterium, sprays an acid electrolyzed functional water every 24-72h; Described acid electrolyzed functional water comprises highly acid electrolyzed functional water (AEFW) or subacidity electrolyzed functional water (SEFW); The pH value of described highly acid electrolyzed functional water is not higher than 3.0, and redox potential is 1000-1200mV, and effective chlorine density is 20-100mg/L; The pH value of described subacidity electrolyzed functional water is 5.0-6.5, and redox potential is 800-1000mV, and effective chlorine density is 20-100mg/L.
2. a kind of acid electrolyzed functional water according to claim 1 suppresses the method for tobacco black shank bacterium, and it is characterized in that: the effective chlorine density of described acid electrolyzed functional water is 60-100mg/L.
3. a kind of acid electrolyzed functional water according to claim 1 suppresses the method for tobacco black shank bacterium, and it is characterized in that: described acid electrolyzed functional water is the subacidity electrolyzed functional water.
4. a kind of acid electrolyzed functional water according to claim 1 suppresses the method for tobacco black shank bacterium, it is characterized in that: the time interval that described acid electrolyzed functional water sprays is 48h.
5. a kind of acid electrolyzed functional water according to claim 1 suppresses the method for tobacco black shank bacterium, and it is characterized in that: be 30s the time of contact of described acid electrolyzed functional water and tobacco black shank bacterium.
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| CN105727323B (en) * | 2014-12-11 | 2018-08-14 | 甘肃惠森药业发展有限公司 | The method for carrying out Chinese medicine sterilizing using electrolyzed functional water |
| CN105794550A (en) * | 2014-12-30 | 2016-07-27 | 徐立江 | Application method of electricity-produced functional water for controlling greenhouse Phytophythora capsici |
| CN105638237A (en) * | 2015-12-28 | 2016-06-08 | 浙江科达生物科技有限公司 | Method of utilizing acidic electrolysis water for preventing and treating diseases and insect pests of ganoderma lucidum |
| CN110540266A (en) * | 2019-09-17 | 2019-12-06 | 山东理工大学 | Air-ground combined aviation plant protection operation system based on electrolyzed functional water |
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| JP2002315457A (en) * | 2001-04-19 | 2002-10-29 | Mitsumasa Urata | Functional water for outdoor and hydroponic culture using carbide, pyroligneous acid, ash and magnet, and integrated system for cultivation |
| FR2825580B1 (en) * | 2001-06-07 | 2004-07-23 | Rhobio | OVER-EXPRESSION OF A LIPOXYGENASE IN PLANTS AND REDUCED SENSITIVITY OF PLANTS TO DISEASES AND ATTACKS BY PATHOGENIC ORGANISMS |
| CN101341869B (en) * | 2008-08-19 | 2011-04-20 | 中国农业大学 | Application of electroproduction functional water in fresh-keeping of fresh cut flower |
| CN101779754B (en) * | 2010-03-23 | 2012-07-04 | 中国农业大学 | Method for clearing away aflatoxin from crop seeds by utilizing acid electrolyzed functional water |
| CN101928349B (en) * | 2010-07-30 | 2012-02-22 | 中国农业大学 | Application of electrolyzed functional water in soaking process of maize starch production |
| CN102010035B (en) * | 2010-11-12 | 2012-07-04 | 山东农业大学 | Immersed electrolysis mixing device |
| CN102106229B (en) * | 2010-11-17 | 2013-04-03 | 熊汉飞 | Method for producing original ecological organic vegetables |
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