CN102405807A - Method by utilizing acid electroproduction functional water to inhibit tobacco blackleg germs - Google Patents
Method by utilizing acid electroproduction functional water to inhibit tobacco blackleg germs Download PDFInfo
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- CN102405807A CN102405807A CN2011102202733A CN201110220273A CN102405807A CN 102405807 A CN102405807 A CN 102405807A CN 2011102202733 A CN2011102202733 A CN 2011102202733A CN 201110220273 A CN201110220273 A CN 201110220273A CN 102405807 A CN102405807 A CN 102405807A
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Abstract
The invention relates to a method by utilizing acid electroproduction functional water to inhibit tobacco blackleg germs, which is characterized in that: acid electroproduction functional water is adequately contacted with tobacco blackleg germs, the contact time is 30s to 300s, the acid electroproduction functional water is sprayed one time every 24h to 72h, and the inhibition rate of the acid electroproduction functional water to the tobacco blackleg germs is calculated by adopting a computer image processing method; and the subacid electroproduction functional water has an inhibition effect on the tobacco blackleg terms, and the inhibition rate can reach more than 50 percent. The production method is simple, the production cost is low, the processing cost is low, and the acid electroproduction functional water utilized by the method is safe, harmless and residue-free and is free from safety potential danager.
Description
Technical field
The present invention relates to the method that a kind of acid electrolyzed functional water suppresses tobacco black shank bacterium, belong to the crop pest control technical field.
Background technology
" electrolyzed functional water (Electrolyzed Functional Water, EFW) ", also claim electrolytic ionic water, electrolyzed oxidizing water and brine electrolysis etc.It is the weak solution of chloride compound and drinking water or hydrochloric acid and drinking water, the acid electrolyzed functional water that under the processing of special electrolysis unit, obtains and the general name of alkaline electrolyzed functional water.Wherein, acid electrolyzed functional water has lower pH value, higher redox potential (Oxidation-Reduction Potential, ORP) with certain effective chlorine density (Available Chlorine Concentration, ACC).It mainly comprises: highly acid electrolyzed functional water (pH < 3.0, ORP>1000 mV, ACC 20-100 mg/L), subacidity electrolyzed functional water (pH 5.0-6.5, ORP 800-1000 mV, ACC 20-100 mg/L).Bactericidal effect receives much attention acid electrolyzed functional water owing to having widely, and is used in a lot of aspects.At present, the application study for acid electrolyzed functional water both at home and abroad mainly concentrates on health care, food processing, agricultural production three big fields, has obtained abundant achievement in research.Japan in 2002 improves people's living condition and classifies acid electrolyzed functional water as the food additives category.
Balck shank is a kind of systematic fungal disease in China's cured tobacco production.Black shank bacterium is a facultative saprophyte; Can be with mycelium, sporangium, zoospore, chlamydospore, the survival of egg spore variform structure; Mainly, generally can survive about 3 years, when next year, flue-cured tobacco field-transplanting phase environmental condition was suitable to survive the winter on resting mycelium body and the invalid body of chlamydospore in soil or muck; Germ will breed in a large number, form the source of dying then.
Black shank is distributed widely in the World tobacco growing area, also is one of main disease on China tobacco.Investigating Shandong, Henan and the Anhui in China the Yellow River and Huai He River cigarette district according to us, is more serious in history balck shank district.Yunnan, Guizhou, Guangdong, Fujian, Hunan are also more general.Also there is generation in the Liaoning in northeast.The incidence of disease of these lesiones is generally 10-15%, and heavy person reaches 50%.Mainly contain to the method that suppresses black shank at present: (1) seed selection and popularization disease-resistant variety; (2) cultural control measure is selected for use anosis strong sprout, efficent rotation, in good time morning to plant, improve cultivation step, is noted the field health during like cultivation; (3) chemical control, 75% thiophanate methyl, 25% metalaxyl, 95% fenaminosulf, 0.3% aliette or 58% metalaxyl MnZn, the many Mils of 53% gold medal thunder.But the method for above-mentioned inhibition balck shank has certain limitation, and as generally investment is big, the cycle is long, and application scale is less, is difficult to use; And that the chemical control method can cause is necessarily residual, and long half time pollutes environment, therefore also has certain shortcoming.
Summary of the invention
The objective of the invention is to defective, a kind of method of utilizing acid electrolyzed functional water to suppress tobacco black shank bacterium is provided to above-mentioned prior art existence.This method is simple, and processing cost is low, and noresidue can obviously suppress tobacco black shank bacterium, is a kind of novel practical, safe and effective procedure.
A kind of method of utilizing acid electrolyzed functional water to suppress tobacco black shank bacterium, concrete scheme is following:
Spray tobacco black shank bacterium with acid electrolyzed functional water, when spraying, be 30-300 s the time of contact of acid electrolyzed functional water and black shank at every turn, every at a distance from the once acid electrolyzed functional water of 24-72 h sprinkling; Described acid electrolyzed functional water comprises highly acid electrolyzed functional water (AEFW) and subacidity electrolyzed functional water (SEFW); The pH value of said highly acid electrolyzed functional water is not higher than 3.0, and redox potential is 1000-1200 mV, and effective chlorine density is 20-100 mg/L; The pH value of said subacidity electrolyzed functional water is 5.0-6.5, and redox potential is 800-1000 mV, and effective chlorine density is 20-100 mg/L.Preferred 60-100 mg/L of the effective chlorine density of said acid electrolyzed functional water.
The preferred subacidity electrolyzed functional water of said acid electrolyzed functional water.
The time interval that said acid electrolyzed functional water sprays is preferred 48 h, promptly whenever sprays once acid electrolyzed functional water at a distance from 48 h.
Said acid electrolyzed functional water needs fully to contact with tobacco black shank bacterium.
The time of contact of said acid electrolyzed functional water and tobacco black shank bacterium preferred 30 s.
Compared with prior art, advantage that the present invention has and beneficial effect are: it is remarkable that (1) the present invention suppresses the tobacco black shank bacterium effect, and inhibiting rate is up to more than 50%; (2) production method of the present invention is simple, production cost is low, and the present invention only need bear the water rate and the electricity charge, so cost is very low; (3) the used acid electrolyzed functional water security of the present invention, harmless can be reduced to light water after use, can not work the mischief to environment, and safety concerning the user does not have nocuity and residual equally.This method is a kind of novel practical, safe and effective procedure.
Another purpose of the present invention has provided and has adopted the Computer Image Processing method to calculate the method for acid electrolyzed functional water to the tobacco black shank bacterium inhibiting rate.
Fig. 2 has shown the bacterium colony of black shank bacterium after cultivating a period of time, calculates the area in first circle through the method for Computer Image Processing
S 1, second circle inner area
S 2, the 3rd circle inner area
S 3Spray for the first time the amplification rate of tobacco black shank bacterium behind the acid electrolyzed functional water:
In the formula
: the amplification rate of spraying for the first time tobacco black shank bacterium behind the acid electrolyzed functional water
S 2-1: the area between first circle and second circle
Spray for the second time the amplification rate of tobacco black shank bacterium behind the acid electrolyzed functional water:
In the formula
: the amplification rate of spraying for the second time tobacco black shank bacterium behind the acid electrolyzed functional water
S 3-2: the area between second circle and the 3rd circle
Calculating the amplification rate of black shank bacterium can eliminate with the method
S 1,
S 2The random error of bringing.
Acid electrolyzed functional water is to the inhibiting rate of tobacco black shank bacterium:
In the formula
: acid electrolyzed functional water is to the inhibiting rate of tobacco black shank bacterium
Description of drawings
Fig. 1 sprays the amplification rate (s times of contact 300) of tobacco black shank bacterium behind the acid electrolyzed functional water of different indexs;
Wherein
XAxle is the different effective chlorine densities of acid electrolyzed functional water, and Control is that the control group effective chlorine density is 0 mg/L,
YAxle is a tobacco black shank bacterium amplification rate;
Fig. 2 sprays the bacterium colony contrast (s times of contact 300) of acid electrolyzed functional water and the tobacco black shank bacterium behind the sterile water of pH6.06, effective chlorine density 91 mg/L;
Cultivate the size of black shank bacterium colony afterwards through 144 h; Be followed successively by first circle, second circle, the 3rd circle from inside to outside; The area that first circle is surrounded is the bacterium colony size that 48 h are cultivated in inoculation afterwards; Area between first circle and second circle is to spray the bacterium colony size of cultivating 48 h behind the subacidity electrolyzed functional water of pH6.06, effective chlorine density 91 mg/L for the first time, is to spray the bacterium colony size of cultivating 48 h behind the subacidity electrolyzed functional water of pH6.06, effective chlorine density 91 mg/L for the second time between the 3rd circle and second circle;
The subacidity electrolyzed functional water of Fig. 3 pH6.06, effective chlorine density 90 mg/L and tobacco black shank bacterium time of contact are to the influence of tobacco black shank bacterium amplification rate;
Wherein
XAxle is the time that the subacidity electrolyzed functional water of pH6.06, effective chlorine density 90 mg/L contacts with tobacco black shank bacterium, and Control is that be 300 s the time of contact of black shank and sterile water,
YAxle is a tobacco black shank bacterium amplification rate;
The subacidity electrolyzed functional water of Fig. 4 pH6.06, effective chlorine density 90 mg/L contacts 30 s and 5 min effect comparison with tobacco black shank bacterium.
Concrete embodiment
The preparation of acid electrolyzed functional water
(1) is that 3% ratio is diluted in concentrated hydrochloric acid in the distilled water according to mass percent, stirs, place the electrolyte bottle of acid electrolyzed functional water process units then;
(2) at voltage 5 V, electric current 8 A, hydrochloric acid solution flow 1.02 L/h, carry out cell reaction under the condition of raw water flow 100 L/h continuously, produce acid electrolyzed functional water; Through detecting the acid electrolyzed functional water of gained is the acid electrolyzed functional water of pH=2.33 ± 0.10, effective chlorine density 90 ± 3 mg/L, redox potential 1187 ± 10 mV.
Embodiment 1 acid electrolyzed functional water is to the inhibition test of tobacco black shank bacterium
(1) test material
Tobacco black shank bacterium.
(2) pre-treatment of test material
At first tobacco black shank bacterium is seeded in and shifts to an earlier date on the ready potato dextrose agar flat board, being placed on temperature is that it is subsequent use to cultivate 48 h in 28 ± 1 ℃ of constant incubators.
(3) test processing
Dilute the acid electrolyzed functional water of preparation or add the acid electrolyzed functional water that sodium bicarbonate solution is prepared following index:
Sterile water (contrast) pH=7.33 ± 0.10, ORP=174 mV, ACC=0 mg/L.
(4) test method
Ready 42 flat boards of inoculating tobacco black shank bacterium that do not have assorted bacterium are divided into 7 groups at random; Go out the size of bacterium colony at this moment at each dull and stereotyped back side with delineating stroke; All put in the superclean bench of the bacterium of having gone out in advance, in superclean bench, the acid electrolyzed functional water of 7 kinds of different indexs is sprayed onto 7 windings respectively and grows tobacco in the flat board of black shank bacterium in the table 1 that will before test, prepare; Sprinkling amount in each flat board is 20 mL; The principle of choosing 20 mL is for the acid electrolyzed functional water that guarantees different indexs can flood whole tobacco black shank bacteriums, just can play antibacterial effect because have only acid electrolyzed functional water to contact with tobacco black shank bacterium, be 300 s time of contact; Pour the acid electrolyzed functional water of the different indexs in each flat board in the waste liquid cylinder into then; Burn dull and stereotyped border with alcolhol burner, prevent pollution microbes, the constant incubator that has the inoculation of handling well the flat board of tobacco black shank bacterium to put into 28 ± 1 ℃ is at last cultivated 48 h (time interval of twice sprinkling also is 48 h); Take out the size of the bacterium colony that draws once more at each dull and stereotyped back side this moment; Repeat above-mentioned steps successively, spray once the acid electrolyzed functional water of 7 kinds of different indexs of preparation in the table 1 again, take out behind 48 h, draw a circle, the amplification rate of tobacco black shank bacterium behind the acid electrolyzed functional water of the different indexs of the each sprinkling of calculating.
(5) acid electrolyzed functional water is to the calculating of tobacco black shank bacterium inhibiting rate
(6) result of the test
Result's (seeing Fig. 1 and Fig. 2), the tobacco black shank bacterium amplification rate of spraying the acid electrolyzed functional waters of different indexs be starkly lower than control group (
p<0.01), the effective chlorine density of subacidity electrolyzed functional water to the influence of the amplification rate of tobacco black shank bacterium extremely significantly (
p<0.01), under same effective chlorine density, different pH to the influence of the amplification rate of tobacco black shank bacterium not significantly (
p>0.05).When effective cl concn reaches 90 mg/L, the amplification rate of spraying tobacco black shank bacterium behind highly acid and the subacidity electrolyzed functional water much at one (
p>0.05); Inhibiting rate is up to more than 50%; Because strong acid can have injury effect to plant, and the test discovery, when effective cl concn reaches 90 mg/L; Highly acid and subacidity electrolyzed functional water are not remarkable to the inhibiting rate influence of black shank, therefore preferred subacidity electrolyzed functional water (pH6.06, ACC=90 mg/L, ORP=922 mV).
Embodiment 2 acid electrolyzed functional waters and tobacco black shank bacterium time of contact are to the influence of tobacco black shank bacterium inhibiting rate
(1) test material
Tobacco black shank bacterium.
(2) test material pre-treatment
See embodiment 1.
(3) test processing
According to the result that embodiment 1 draws, adopt the subacidity electrolyzed functional water to make an experiment, add the subacidity electrolyzed functional water that sodium bicarbonate solution is prepared following index to the highly acid electrolyzed functional water of preparation:
Subacidity electrolyzed functional water: pH=6.06, redox potential ORP=922 mV, effective chlorine density ACC=90 mg/L;
Sterile water (contrast) pH=7.33 ± 0.10, ORP=174 mV, ACC=0 mg/L.
(4) test method
Ready 30 aseptic flat boards of inoculating tobacco black shank bacterium are divided into 5 groups at random; Go out the size of bacterium colony at this moment at each dull and stereotyped back side with delineating stroke; All put in the superclean bench of the bacterium of having gone out in advance, in superclean bench, the subacidity electrolyzed functional water that will before test, prepare is sprayed onto wherein 4 windings respectively and grows tobacco in the flat board of black shank bacterium; Sprinkling amount in each flat board is 20 mL; The principle of choosing 20 mL is in order to guarantee that the subacidity electrolyzed functional water floods whole tobacco black shank bacteriums, just can play antibacterial effect because have only acid electrolyzed functional water to contact with tobacco black shank bacterium, to be respectively 30 s, 60 s, 120 s, 300 s 4 groups time of contact; Control group sprays 20 mL sterile waters, and be 300 s time of contact.Finishing the back time of contact pours the subacidity electrolyzed functional water in each flat board in the waste liquid cylinder into; Burn dull and stereotyped border with alcolhol burner; Prevent pollution microbes; The constant incubator that has the inoculation of handling well the flat board of tobacco black shank bacterium to put into 28 ± 1 ℃ is at last cultivated 48 h (time interval of twice sprinkling also is 48 h), takes out the size of drawing bacterium colony this moment at each dull and stereotyped back side once more; After repeating above-mentioned steps 3 times more successively, the size that all will draw bacterium colony at the dull and stereotyped back side before each the sprinkling is calculated each amplification rate of spraying tobacco black shank bacterium behind the subacidity electrolyzed functional water.
(5) acid electrolyzed functional water is to the calculating of tobacco black shank bacterium inhibiting rate
(6) result of the test
The result shows (seeing Fig. 3 and Fig. 4), spray behind the subacidity electrolyzed functional water tobacco black shank bacterium amplification rate significantly be lower than control group (
p<0.01), inhibiting rate is up to more than 50%, subacidity electrolyzed functional water time of contact different with black shank bacterium to the influence of the amplification rate of tobacco black shank bacterium not significantly (
p>0.05).In practical application; Can adopt the mode of spraying or irrigating to realize the control of black shank; And these two kinds of methods of application can guarantee that subacidity electrolyzed functional water and germ have sufficient time of contact, but according to experimental result, time of contact is not remarkable to the bacteriostasis rate influence; Therefore, subacidity electrolyzed functional water and tobacco black shank bacterium time of contact can preferred 30 s.
What need explain at last is; Above embodiment is only unrestricted in order to the explanation technical scheme; Although the present invention is specified with reference to preferred embodiment; Those skilled in the art can make amendment or is equal to replacement technical scheme of the present invention, and does not break away from the spirit and the scope of technical scheme of the present invention, and it all should be encompassed in the middle of the claim scope of the present invention.
Claims (5)
1. an acid electrolyzed functional water suppresses the method for tobacco black shank bacterium; It is characterized in that: spray tobacco black shank bacterium with acid electrolyzed functional water; Acid electrolyzed functional water needs to contact with tobacco black shank bacterium; During each the sprinkling, be 30-300 s the time of contact of acid electrolyzed functional water and black shank, every at a distance from the once acid electrolyzed functional water of 24-72 h sprinkling; Described acid electrolyzed functional water comprises highly acid electrolyzed functional water (AEFW) and subacidity electrolyzed functional water (SEFW); The pH value of said highly acid electrolyzed functional water is not higher than 3.0, and redox potential is 1000-1200 mV, and effective chlorine density is 20-100 mg/L; The pH value of said subacidity electrolyzed functional water is 5.0-6.5, and redox potential is 800-1000 mV, and effective chlorine density is 20-100 mg/L.
2.
According to claim 1A kind of acid electrolyzed functional water suppresses the method for tobacco black shank bacterium, and it is characterized in that: the effective chlorine density of said acid electrolyzed functional water is 60-100 mg/L.
3.
According to claim 1A kind of acid electrolyzed functional water suppresses the method for tobacco black shank bacterium, and it is characterized in that: said acid electrolyzed functional water is the subacidity electrolyzed functional water.
4.
According to claim 1A kind of acid electrolyzed functional water suppresses the method for tobacco black shank bacterium, it is characterized in that: the time interval that said acid electrolyzed functional water sprays is 48 h.
5.
According to claim 1A kind of acid electrolyzed functional water suppresses the method for tobacco black shank bacterium, and it is characterized in that: be 30 s the time of contact of said acid electrolyzed functional water and tobacco black shank bacterium.
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Cited By (4)
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| CN105638237A (en) * | 2015-12-28 | 2016-06-08 | 浙江科达生物科技有限公司 | Method of utilizing acidic electrolysis water for preventing and treating diseases and insect pests of ganoderma lucidum |
| CN105727323A (en) * | 2014-12-11 | 2016-07-06 | 甘肃惠森药业发展有限公司 | A method of sterilizing traditional Chinese medicine materials by utilizing electrolyzed functional water |
| CN105794550A (en) * | 2014-12-30 | 2016-07-27 | 徐立江 | Application method of electricity-produced functional water for controlling greenhouse Phytophythora capsici |
| CN110540266A (en) * | 2019-09-17 | 2019-12-06 | 山东理工大学 | Air-ground combined aviation plant protection operation system based on electrolyzed functional water |
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| CN110540266A (en) * | 2019-09-17 | 2019-12-06 | 山东理工大学 | Air-ground combined aviation plant protection operation system based on electrolyzed functional water |
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