CN102912000A - Method for biologically dissolving limestone by quantitative microalgae - Google Patents
Method for biologically dissolving limestone by quantitative microalgae Download PDFInfo
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- CN102912000A CN102912000A CN 201210439970 CN201210439970A CN102912000A CN 102912000 A CN102912000 A CN 102912000A CN 201210439970 CN201210439970 CN 201210439970 CN 201210439970 A CN201210439970 A CN 201210439970A CN 102912000 A CN102912000 A CN 102912000A
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Abstract
The invention discloses a method for biologically dissolving limestone by quantitative microalgae. A part of magnesium ions dissolved out from limestone are absorbed and utilized by the microalgae, and a part of magnesium ions are used for changing the concentration of magnesium ions in the solution; a biological growth rate equation multiplied by a time integral of time represents the microalgae biological dissolution accumulation time in a culture solution per unit volume; the equation that the dissolution quantity of limestone magnesium by microalgae in the culture solution per unit volume varies along with the microalgae biological dissolution accumulation time represents the dissolution quantity of limestone magnesium by microalgae in the culture solution per unit volume under different microalgae biological dissolution accumulation times, and the derivation of the equation represents the dissolution quantity of limestone magnesium by microalgae per unit biomass and unit time; and the dissolution of magnesium also directly reflects the dissolution of limestone, so that the dissolution quantity of limestone magnesium by microalgae per unit biomass and unit time can quantitatively reflect the biological dissolution of limestone by microalgae. The invention fills the blank for biologically dissolving limestone by quantitative microalgae, and the method has mature technologies, easiness in operation and low cost.
Description
Technical field
The present invention relates to a kind of quantitative little algae to the bioerodable action method of limestone, belong to ecological environment treatment monitoring and engineering geology monitoring field.
Background technology
The corrosion of limestone comprises physical action, chemical action and biological action.Limestone often suffers biological corrosion on every side, and therefore, the bioerodable effect is most important.The karst region rock surface has abundant algae to assemble, and a large amount of algae corrode the rock in substrate and the embankment in the water body.
Although someone carried out research to algae, lichens, liver moss to the little form of biokarst and retentiveness that the corrosion of the shallow top layer of limestone forms, the people is also arranged, and the effect in the karstification process is studied to microorganism and carbonic anhydrase.But the quantitative examination to " biological bioerodable effect to limestone " this complex process yet there are no report.
Little algae (microalgae) comprises that all live in the swim microphyte of mode of life of Shui Zhongying, usually just refers to planktonic algae.Little algal structure is simple, and its physiological process is also relatively simple, and some kind is the model plant of scientific research, as: Chlamydomonas reinhardtii, chlorella, all right artificial culture of many types, this research for us is provided convenience.Little algae equally also comprises physical action, chemical action and biological action to the Limestone Dissolution effect.Different algae is obviously different to the bioerodable effect of limestone, has the algae of the outer carbonic anhydrase of born of the same parents to have stronger corrosion effect with comparing without the algae of the outer carbonic anhydrase of born of the same parents.At present, the corrosion effect of the little algae of different sorts quantitatively is a very stubborn problem.
Can produce a large amount of calcium ions and magnesium ions in little algae in to the bioerodable mechanism of limestone, these calcium ions and magnesium ions parts absorb for algae, and a part exists in solution, and some precipitates in sedimentary mode.Because the calcium magnesium mass ratio of limestone is generally greater than 40:1, and the calcium magnesium mass ratio that frond utilizes is less than 10:1, and therefore, magnesium ion becomes the limiting factor of micro algae growth.When algae utilizes the magnesium of stripping from limestone, although some calcium is also utilized by algae, but overwhelming majority's calcium of stripping from limestone is not utilized, or exist in solution with ionic species, or be deposited in the solution with the precipitation of calcium carbonate form, and the calcium carbonate quantity that precipitates calculation more difficult to estimate, magnesium is less with respect to the impact that calcium is precipitated.Therefore, we can treat by mensuration the change in concentration of content and the magnesium in the solution of magnesium in the micrometer algae, by the bioerodable effect of the series of computation unit of obtaining frond unit time to limestone.
Quantitative little algae yet there are no report to the method for limestone bioerodable effect at present, and quantitatively very important to the effect of limestone bioerodable of little algae, it can assess the little algae in river, lake effectively to the harm of the buildings such as karst region ecotope and dykes and dams, thereby reaches the purpose of prediction disaster.Particularly current, in the serious situation of numerous lake eutrophications, seem even more important and urgent.
Summary of the invention
The technical problem to be solved in the present invention is, the quantivative approach of a kind of little algae to the effect of limestone bioerodable is provided, and fills up quantitative little algae to the blank of the bioerodable effect of limestone.
The present invention takes following technical scheme: a kind of quantitative little algae is characterized in that: comprise following steps the bioerodable action method of limestone:
First, measure limestone powder calcium magnesium mass ratio, in two groups of cellar culture liquid, add respectively excessive calcium magnesium mass ratio greater than 40 limestone powder and cultivate after not adding the limestone powder and treat the micrometer algae, organize at another again and only add this calcium magnesium mass ratio in nutrient solution and do not add frond greater than 40 limestone powder;
Second, treat that the micrometer algae is being cultivated under the culture condition of investigating, behind different incubation times, measure respectively and be added with the chlorophyll-a concentration that limestone powder and cultivation have the nutrient solution of frond, the magnesium ion concentration of corresponding incubation time solution and only add the magnesium ion concentration that the limestone powder is not cultivated solution in the nutrient solution that frond is arranged, come the normalization method calibration to be added with the magnesium ion concentration that limestone powder and cultivation have solution in the nutrient solution of corresponding incubation time of frond with the magnesium ion concentration of the solution of the initial magnesium ion concentration of solution in the nutrient solution that only adds the limestone powder and do not cultivate frond and its different time, be added with limestone powder and the cultivation that obtain after the corresponding incubation time calibration have magnesium ion concentration in the nutrient solution of frond; There are magnesium ion concentration data in the nutrient solution of frond to deduct the magnesium ion concentration of its initial solution be added with limestone powder and cultivation after the calibration of different incubation times again, draw and be added with the changing value that limestone powder and cultivation have magnesium ion concentration in the nutrient solution of frond under the different incubation times;
The 3rd, to wait to cultivate more than 6 days, the frond that results are cultivated in not adding limestone powder nutrient solution is measured the content of this frond magnesium and the Chlorophyll-a Content of correspondence, and Mg content corresponding to Units of Account quality chlorophyll a;
The 4th, multiply by Mg content corresponding to unit mass chlorophyll a after the chlorophyll-a concentration value that is added with limestone powder and cultivation under the different incubation times and has the nutrient solution of frond deducted its initial chlorophyll-a concentration value, obtain the magnesium amount from the limestone stripping that unit volume nutrient solution frond absorbs under the different incubation times;
The 5th, the changing value that is added with limestone powder and cultivation under the different incubation times and has magnesium ion concentration in the nutrient solution of frond is added the magnesium amount from the limestone stripping that unit volume nutrient solution frond absorbs under the corresponding incubation time, obtain different incubation time unit volume nutrient solution fronds to the stripping quantity of limestone magnesium;
The 6th, according to being added with limestone powder and cultivation under the different incubation times nutrient solution chlorophyll-a concentration data of frond are arranged, make up the exponential growth equation that the chlorophyll-a concentration of little algae under culture condition to be measured changes with incubation time, and to the equation differentiate of chlorophyll-a concentration with the incubation time variation, obtain little algae biological growth rate equation;
The 7th, little algae biological growth rate equation be multiply by the time to time integral, obtain the little algae bioerodable of unit volume nutrient solution cumulative function equation of time, and calculate under the different incubation times little algae bioerodable cumulative function time in the unit volume nutrient solution;
The 8th, make up the equation that different incubation time fronds in the unit volume nutrient solution change with little algae bioerodable cumulative function time of correspondence the stripping quantity of limestone magnesium, and to this equation differentiate, draw frond to be measured under unit time unit's Chlorophyll-a Content to the bioerodable effect of limestone;
The 9th, little algae of unit time unit's Chlorophyll-a Content is carried out the corrosion amount to the bioerodable effect of limestone convert, obtain little algae of unit time unit's Chlorophyll-a Content to the corrosion amount of limestone; Little algae that concrete conversion method is unit time unit's Chlorophyll-a Content be multiply by corrosion amount conversion factor F to the bioerodable effect of limestone, and corrosion amount conversion factor F represents with following corrosion amount conversion equation:
F=
B in the above-mentioned corrosion amount conversion equation
MgAnd B
CaBe respectively the used limestone magnesium of experiment and the shared mass ratio of calcium; M
MgCO3And M
CaCO3Be respectively the molecular mass of magnesiumcarbonate and calcium carbonate; M
MgAnd M
CaBe respectively the molecular mass of magnesium and calcium.
Advantage of the present invention is as follows:
Quantitatively little algae is to the principle of the bioerodable effect of limestone the unit frond unit time:
The magnesium part of limestone stripping absorbs for frond, a part causes the magnesium ion concentration in the solution to change, the stripping quantity of limestone magnesium corresponding to frond bioerodable cumulative function time in the unit volume nutrient solution is representing in the unit volume nutrient solution corrosion situation of limestone under the total cumulative time of frond bioerodable effect.Therefore, can utilize frond bioerodable cumulative function time in the unit volume nutrient solution and corresponding little algae to the derivative of the stripping quantity equation of limestone magnesium, the unit's of acquisition frond unit time, little algae was to the bioerodable effect to limestone.
The magnesium ion part of limestone stripping absorbs for frond, a part causes the magnesium ion concentration in the solution to change, and therefore the magnesium ion concentration changing value of different incubation time solution adds that the unit volume nutrient solution frond magnesium ion amount of absorbing is representing in the unit volume nutrient solution little algae to the stripping quantity of limestone magnesium.Time integral is being represented the frond bioerodable cumulative function time in the unit volume nutrient solution with the biological growth rate equation time of multiply by.The equation that the little algae of unit volume nutrient solution changes with the frond bioerodable cumulative function time the stripping quantity of limestone magnesium representing different frond bioerodable under the cumulative function time in the unit volume nutrient solution little algae then the stripping quantity of limestone magnesium has then been represented the stripping quantity of unit biomass unit time frond to limestone magnesium to its differentiate.And the stripping of magnesium has directly reflected the corrosion of limestone, thus the unit biomass unit time little algae can reflect quantitatively that to the stripping quantity of limestone magnesium little algae is to the bioerodable effect of limestone.
Advantage of the present invention is as follows:
1) the quantitative little algae of present method energy has been filled up quantitative little algae to the blank of limestone bioerodable effect to the bioerodable effect of limestone;
2) present method only need to be measured chlorophyll-a concentration and Mg content, and technology maturation is simple to operate, and cost is low;
3) present method is carried out culture experiment under identical experiment condition, therefore, obtains little algae more reliable to the data of the bioerodable effect of limestone.
Embodiment
Embodiments of the invention: first step, measure the calcium magnesium mass ratio that will add the limestone powder, in two groups of cellar culture liquid, add respectively excessive calcium magnesium mass ratio greater than 40 limestone powder (less than 60 orders) and cultivate after not adding the limestone powder and treat the micrometer algae, organize at another again and only add this calcium magnesium mass ratio in nutrient solution and do not add frond greater than 40 limestone powder;
Second step is treated the micrometer algae being cultivated under the culture condition of investigating, and behind different incubation times, measures respectively and is added with the chlorophyll-a concentration CHLa that limestone powder and cultivation have the nutrient solution of frond
i, corresponding incubation time solution magnesium ion concentration CMg
iOnly add the limestone powder and do not cultivate the magnesium ion concentration CO of solution in the nutrient solution that frond is arranged
i, do not cultivate the initial magnesium ion concentration CO of solution in the nutrient solution that frond is arranged with only adding the limestone powder
0Solution magnesium ion concentration CO with its different time
iCome normalization method calibration to be added with the magnesium ion concentration CMg that limestone powder and cultivation have solution in the nutrient solution of corresponding incubation time of frond
i, be added with limestone powder and the cultivation that obtain after the calibration of corresponding incubation time have magnesium ion concentration CTMg in the nutrient solution of frond
i, calibration equation is CTMg
i=CMg
iCO
0/ CO
iAgain be added with limestone powder and cultivation after the calibration of different incubation times there is magnesium ion concentration data CTMg in the nutrient solution of frond
iDeduct the magnesium ion concentration CTMg of its initial solution
0, draw and be added with the changing value δ CTMg that limestone powder and cultivation have magnesium ion concentration in the nutrient solution of frond under the different incubation times
i
Third step was waited to cultivate more than 6 days, and the frond that results are cultivated in not adding limestone powder nutrient solution is measured the content of this frond magnesium and the Chlorophyll-a Content of correspondence, and Mg content CMgPChl corresponding to Units of Account quality chlorophyll a;
The 4th step has the chlorophyll-a concentration value CHLa of the nutrient solution of frond with being added with limestone powder and cultivation under the different incubation times
iDeduct its initial chlorophyll-a concentration value CHLa
0After multiply by Mg content CMgPChl corresponding to unit mass chlorophyll a, obtain the magnesium amount Qa from the limestone stripping that unit volume nutrient solution frond absorbs under the different incubation times
i
The 5th step will be added with limestone powder and cultivation and have the changing value δ CTMg of magnesium ion concentration in the nutrient solution of frond under the different incubation times
iAdd the magnesium amount Qa from the limestone stripping that unit volume nutrient solution frond absorbs under the corresponding incubation time
i, obtain different incubation time unit volume nutrient solution fronds to the stripping quantity Q of limestone magnesium
i
The 6th step has the nutrient solution chlorophyll-a concentration CHLa of frond according to being added with limestone powder and cultivation under the different incubation times
iData make up the exponential growth equation that the chlorophyll-a concentration of little algae under culture condition to be measured changes with incubation time T, and to chlorophyll-a concentration CHLa
iEquation differentiate with incubation time T changes obtains little algae biological growth rate Vch equation;
The 7th step, little algae biological growth rate Vch equation be multiply by after the time time integral, obtain the little algae bioerodable of unit volume nutrient solution cumulative function equation of time, and calculate under the different incubation times little algae bioerodable cumulative function time PT in the unit volume nutrient solution
i
The 8th step makes up the different incubation time fronds of unit volume nutrient solution to the stripping quantity Q of limestone magnesium
iLittle algae bioerodable cumulative function time PT with correspondence
iThe equation that changes, and to this equation differentiate, derivative be multiply by the unit conversion factor carries out unit conversion, draws frond to be measured under unit time unit's Chlorophyll-a Content to the bioerodable effect E of limestone;
The 9th step. little algae of unit time unit's Chlorophyll-a Content is carried out the conversion of corrosion amount to the bioerodable effect E of limestone, obtain the little algae of unit time unit's Chlorophyll-a Content to the corrosion amount M of limestone; Little algae that concrete conversion method is unit time unit's Chlorophyll-a Content be multiply by corrosion amount conversion factor F to the bioerodable effect E of limestone; Corrosion amount conversion factor F represents with following corrosion amount conversion equation:
F=
B in the above-mentioned corrosion amount conversion equation
MgAnd B
CaBe respectively the used limestone magnesium of experiment and the shared mass ratio of calcium; M
MgCO3And M
CaCO3Be respectively the molecular mass of magnesiumcarbonate and calcium carbonate; M
MgAnd M
CaBe respectively the molecular mass of magnesium and calcium.
Chlamydomonas is measured the bioerodable effect of limestone:
Cultivated material is: chlamydomonas.Basic culture solution adopts SE substratum (not adding soil extract), and basic culture condition is: photoperiod L/D:12h/12h; 25 ℃ of temperature; Intensity of illumination is
PH value 7.0(regulates with hydrochloric acid and sodium hydroxide).Cultivate chlamydomonas to be measured after in two groups of nutrient solutions, adding respectively calcium magnesium mass ratio that 1g measured and be the limestone powder (less than 60 orders) of 90:1 and not adding the limestone powder, organize at another again and only add same this limestone powder 1g in nutrient solution and do not add chlamydomonas.After chlamydomonas is cultivated 0,1,3,5,7,9 day, measure respectively the nutrient solution Determination of Chlorophyll a concentration C HLa that is added with the limestone powder
iThe magnesium ion concentration CMg of (table 1), solution
i(table 2) and only add the magnesium ion concentration CO of solution in the nutrient solution of limestone powder
i, and with the initial magnesium ion concentration CO that only adds solution in the limestone powder nutrient solution
0Magnesium ion concentration CO with the solution of its different time
iCome normalization method calibration to be added with the magnesium ion concentration CMg that limestone powder and cultivation have solution in the nutrient solution of corresponding incubation time of chlamydomonas
i, be added with limestone powder and the cultivation that obtain after the calibration of corresponding incubation time have magnesium ion concentration CTMg in the nutrient solution of chlamydomonas
i(table 2), calibration equation are CTMg
i=CMg
iCO
0/ CO
iAgain be added with limestone powder and cultivation after the calibration of different incubation times there is magnesium ion concentration data CTMg in the nutrient solution of chlamydomonas
iDeduct the magnesium ion concentration CTMg of its starting soln
0, draw and be added with the changing value δ CTMg that limestone powder and cultivation have magnesium ion concentration in the nutrient solution of chlamydomonas under the different incubation times
i(table 3).Results are cultivated after 9 days and are not added the chlamydomonas of cultivating in the limestone powder nutrient solution, measure its Mg content and Chlorophyll-a Content, and Mg content CMgPChl corresponding to Units of Account quality chlorophyll a.With being added with limestone powder and cultivation under the different incubation times chlorophyll-a concentration value CHLa of the nutrient solution of chlamydomonas is arranged
iDeduct its initial chlorophyll-a concentration value CHLa
0After multiply by Mg content CMgPChl corresponding to unit mass chlorophyll a, obtain the magnesium amount Qa from the limestone stripping that unit volume nutrient solution chlamydomonas absorbs under the different incubation times
i(table 3).
To be added with limestone powder and cultivation under the different incubation times and have the changing value δ CTMg of magnesium ion concentration in the nutrient solution of chlamydomonas
iAdd the magnesium amount Qa from the limestone stripping that unit volume nutrient solution chlamydomonas absorbs under the corresponding incubation time
i, obtain different incubation time unit volume nutrient solution chlamydomonas to the stripping quantity Q of limestone magnesium
i(table 3).
Make up the exponential growth equation of in time T variation of the chlorophyll-a concentration of chlamydomonas under culture condition to be measured, and to chlorophyll-a concentration CHLa
iWith the equation differentiate that incubation time T changes, obtain chlamydomonas biological growth rate Vch equation, chlamydomonas biological growth rate Vch equation be multiply by the time to time integral, obtain unit volume nutrient solution chlamydomonas bioerodable cumulative function equation of time (table 4).
According to unit volume nutrient solution chlamydomonas bioerodable cumulative function equation of time, calculate under the different incubation times chlamydomonas bioerodable cumulative function time PT in the unit volume nutrient solution
i, such as table (5).
Construct straight-line equation by table 5: Q
i=0.61+8.47 * 10
-5PT
i(R
2=0.964, P=0.0029), this equation differentiate is got slope 8.47 * 10
-5 
, slope be multiply by the unit conversion factor 3.6 * 10
5, carry out unit conversion, obtain E=30.49
, then be that the chlamydomonas of unit time unit's Chlorophyll-a Content is to the bioerodable effect E of limestone.Again the chlamydomonas of unit time unit's Chlorophyll-a Content be multiply by corrosion amount conversion factor F to the bioerodable effect E of limestone.Corrosion amount conversion factor F represents with following corrosion amount conversion equation:
F=
B in the above-mentioned corrosion amount conversion equation
MgAnd B
CaBe respectively the used limestone magnesium of experiment and the shared mass ratio of calcium; M
MgCO3And M
CaCO3Be respectively the molecular mass of magnesiumcarbonate and calcium carbonate; M
MgAnd M
CaBe respectively the molecular mass of magnesium and calcium.
F is 228.50, and therefore, the chlamydomonas that contains the 1g Chlorophyll-a Content every year is 6966.97 to the corrosion amount M of limestone
, the limestone that the chlamydomonas that also namely contains the 1g Chlorophyll-a Content can corrosion 6966.97 grams in year.
Chlorella is to the mensuration of the bioerodable effect of limestone:
Cultivated material is: chlorella.Basic culture solution adopts SE substratum (not adding soil extract), and basic culture condition is: photoperiod L/D:12h/12h; 25 ℃ of temperature; Intensity of illumination is
PH value 7.0(regulates with hydrochloric acid and sodium hydroxide).Cultivate chlorella to be measured after in two groups of nutrient solutions, adding respectively calcium magnesium mass ratio that 1g measured and be the limestone powder (less than 60 orders) of 90:1 and not adding the limestone powder, organize at another again and only add same this limestone powder 1g in nutrient solution and do not add chlorella.After chlorella is cultivated 0,1,3,5,7,9 day, measure respectively the nutrient solution Determination of Chlorophyll a concentration C HLa that is added with the limestone powder
iThe magnesium ion concentration CMg of (table 6), solution
i(table 7) and only add the magnesium ion concentration CO of solution in the nutrient solution of limestone powder
i, and with the initial magnesium ion concentration CO of solution that only adds in the limestone powder nutrient solution
0Magnesium ion concentration CO with the solution of its different time
iCome normalization method calibration to be added with the magnesium ion concentration CMg that limestone powder and cultivation have solution in the nutrient solution of corresponding incubation time of chlorella
i, be added with limestone powder and the cultivation that obtain after the calibration of corresponding incubation time have CTMg in the nutrient solution of chlorella
i(table 7), calibration equation are CTMg
i=CMg
iCO
0/ CO
iTo be added with limestone powder and cultivation after the calibration of different incubation times and have magnesium ion concentration data CTMg in the nutrient solution of chlorella again
iDeduct the magnesium ion concentration CTMg of its initial solution
0, draw and be added with the changing value δ CTMg that limestone powder and cultivation have magnesium ion concentration in the nutrient solution of chlorella under the different incubation times
i(table 8).Results are cultivated after 9 days and are not added the chlorella of cultivating in the limestone powder nutrient solution, measure its Mg content and Chlorophyll-a Content, and Mg content CMgPChl corresponding to Units of Account quality chlorophyll a.With being added with limestone powder and cultivation under the different incubation times chlorophyll-a concentration value CHLa of the nutrient solution of chlorella is arranged
iDeduct its initial chlorophyll-a concentration value CHLa
0After multiply by Mg content CMgPChl corresponding to unit mass chlorophyll a, obtain the magnesium amount Qa from the limestone stripping that unit volume nutrient solution chlorella absorbs under the different incubation times
i(table 8).
To be added with limestone powder and cultivation under the different incubation times and have the changing value δ CTMg of magnesium ion concentration in the nutrient solution of chlorella
iAdd the magnesium amount Qa from the limestone stripping that unit volume nutrient solution chlorella absorbs under the corresponding incubation time
i, obtain in the different incubation time unit volume nutrient solutions chlorella to the stripping quantity Q of limestone magnesium
i(table 8).
Make up the exponential growth equation of in time T variation of the chlorophyll-a concentration of chlorella under culture condition to be measured, and to chlorophyll-a concentration CHLa
iWith the equation differentiate that incubation time T changes, obtain chlorella biological growth rate Vch equation, chlorella biological growth rate Vch equation be multiply by the time to time integral, obtain unit volume nutrient solution chlorella bioerodable cumulative function equation of time (table 9).
According to unit volume nutrient solution chlorella bioerodable cumulative function equation of time, calculate under the different incubation times chlorella bioerodable cumulative function time PT in the unit volume nutrient solution
i, such as table (10).
Construct straight-line equation by table 10: Q
i=0.66+9.48 * 10
-5PT
i(R
2=0.962, P=0.0032), this equation differentiate is got slope 9.48 * 10
-5 
, slope be multiply by the unit conversion factor 3.6 * 10
5, carry out unit conversion, obtain E=34.13
, then be that the chlorella of unit time unit's Chlorophyll-a Content is to the bioerodable effect E of limestone.Again the chlorella of unit time unit's Chlorophyll-a Content be multiply by corrosion amount conversion factor F to the bioerodable effect E of limestone.Corrosion amount conversion factor F represents with following corrosion amount conversion equation:
F=
B in the above-mentioned corrosion amount conversion equation
MgAnd B
CaBe respectively the used limestone magnesium of experiment and the shared mass ratio of calcium; M
MgCO3And M
CaCO3Be respectively the molecular mass of magnesiumcarbonate and calcium carbonate; M
MgAnd M
CaBe respectively the molecular mass of magnesium and calcium.
F is 228.50, and therefore, the chlorella that contains the 1g Chlorophyll-a Content every year is 7798.71 to the corrosion amount M of limestone
, the limestone that the chlorella that also namely contains the 1g Chlorophyll-a Content can corrosion 7798.71 grams in year.
Effect:
In summary it can be seen, chlorella to the bioerodable effect of limestone greater than the bioerodable effect of chlamydomonas to limestone, this be because the cell of chlorella less than the chlamydomonas cell; With the frond of volume, chlorella has larger surface-area, therefore, has the chlorella of larger surface-area, and the area of contact limestone is large, causes its bioerodable effect also just large.In addition, the chlamydomonas that contains the 1g chlorophyll a limestone that can corrosion 6966.97 grams in the year and the chlorella that contains the 1g chlorophyll a can corrosion 7798.71 grams in year the experimental result of limestone also tally with the actual situation, show that the little algae that utilizes the present invention to obtain is believable to limestone bioerodable effects of action.
Claims (1)
1. a quantitative little algae is characterized in that: comprise following steps the bioerodable action method of limestone:
First, measure limestone powder calcium magnesium mass ratio, in two groups of cellar culture liquid, add respectively excessive calcium magnesium mass ratio greater than 40 limestone powder and cultivate after not adding the limestone powder and treat the micrometer algae, organize at another again and only add this calcium magnesium mass ratio in nutrient solution and do not add frond greater than 40 limestone powder;
Second, treat that the micrometer algae is being cultivated under the culture condition of investigating, behind different incubation times, measure respectively and be added with the chlorophyll-a concentration that limestone powder and cultivation have the nutrient solution of frond, the magnesium ion concentration of corresponding incubation time solution and only add the magnesium ion concentration that the limestone powder is not cultivated solution in the nutrient solution that frond is arranged, come normalization method to be aligned in to be added with limestone powder and cultivation that the magnesium ion concentration of solution in the nutrient solution of corresponding incubation time of frond is arranged with the magnesium ion concentration of the solution of the initial magnesium ion concentration of solution in the nutrient solution that only adds the limestone powder and do not cultivate frond and its different time, be added with limestone powder and the cultivation that obtain after the corresponding incubation time calibration have magnesium ion concentration in the nutrient solution of frond; There are magnesium ion concentration data in the nutrient solution of frond to deduct the magnesium ion concentration of its initial solution be added with limestone powder and cultivation after the calibration of different incubation times again, draw and be added with the changing value that limestone powder and cultivation have magnesium ion concentration in the nutrient solution of frond under the different incubation times;
The 3rd, to wait to cultivate more than 6 days, the frond that results are cultivated in not adding limestone powder nutrient solution is measured the content of this frond magnesium and the Chlorophyll-a Content of correspondence, and Mg content corresponding to Units of Account quality chlorophyll a;
The 4th, multiply by Mg content corresponding to unit mass chlorophyll a after the chlorophyll-a concentration value that is added with limestone powder and cultivation under the different incubation times and has the nutrient solution of frond deducted its initial chlorophyll-a concentration value, obtain the magnesium amount from the limestone stripping that unit volume nutrient solution frond absorbs under the different incubation times;
The 5th, the changing value that is added with limestone powder and cultivation under the different incubation times and has magnesium ion concentration in the nutrient solution of frond is added the magnesium amount from the limestone stripping that unit volume nutrient solution frond absorbs under the corresponding incubation time, obtain different incubation time unit volume nutrient solution fronds to the stripping quantity of limestone magnesium;
The 6th, according to being added with limestone powder and cultivation under the different incubation times nutrient solution chlorophyll-a concentration data of frond are arranged, make up the exponential growth equation that the chlorophyll-a concentration of little algae under culture condition to be measured changes with incubation time, and to the equation differentiate of chlorophyll-a concentration with the incubation time variation, obtain little algae biological growth rate equation;
The 7th, little algae biological growth rate equation be multiply by the time to time integral, obtain the little algae bioerodable of unit volume nutrient solution cumulative function equation of time, and calculate under the different incubation times little algae bioerodable cumulative function time in the unit volume nutrient solution;
The 8th, make up the equation that different incubation time fronds in the unit volume nutrient solution change with little algae bioerodable cumulative function time of correspondence the stripping quantity of limestone magnesium, and to this equation differentiate, draw frond to be measured under unit time unit's Chlorophyll-a Content to the bioerodable effect of limestone;
The 9th, little algae of unit time unit's Chlorophyll-a Content is carried out the corrosion amount to the bioerodable effect of limestone convert, obtain little algae of unit time unit's Chlorophyll-a Content to the corrosion amount of limestone; Little algae that concrete conversion method is unit time unit's Chlorophyll-a Content be multiply by corrosion amount conversion factor F to the bioerodable effect of limestone, and corrosion amount conversion factor F represents with following corrosion amount conversion equation:
F=
B in the above-mentioned corrosion amount conversion equation
MgAnd B
CaBe respectively the used limestone magnesium of experiment and the shared mass ratio of calcium; M
MgCO3And M
CaCO3Be respectively the molecular mass of magnesiumcarbonate and calcium carbonate; M
MgAnd M
CaBe respectively the molecular mass of magnesium and calcium.
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| CN201210577576.5A CN103173520B (en) | 2012-11-07 | 2012-12-27 | Bioerosion action method of quantitative microalgae to limestone |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107941649A (en) * | 2017-11-15 | 2018-04-20 | 青海民族大学 | The detection method of the inorganic nitrogen uptake rate of common microalgae in aquaculture pond |
| CN108364096A (en) * | 2018-02-06 | 2018-08-03 | 长江大学 | The clastic reservoir rock corrosion of Kernel-based methods response increases hole and measures quantitative forecasting technique |
| CN108733985A (en) * | 2018-06-08 | 2018-11-02 | 中国科学院城市环境研究所 | A kind of method of the determining critical environments parameter for restricting microalgae arsenic accumulation capability |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107219349B (en) * | 2017-05-24 | 2019-05-07 | 中国科学院地球化学研究所 | A kind of quantitative microalgae utilizes the inorganic carbon capability approach derived from Silicate Rocks weathering |
| CN107058462B (en) * | 2017-05-31 | 2020-08-11 | 中国科学院地球化学研究所 | Method for quantitatively corroding olivine organisms by microalgae |
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- 2012-12-27 CN CN201210577576.5A patent/CN103173520B/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107941649A (en) * | 2017-11-15 | 2018-04-20 | 青海民族大学 | The detection method of the inorganic nitrogen uptake rate of common microalgae in aquaculture pond |
| CN108364096A (en) * | 2018-02-06 | 2018-08-03 | 长江大学 | The clastic reservoir rock corrosion of Kernel-based methods response increases hole and measures quantitative forecasting technique |
| CN108364096B (en) * | 2018-02-06 | 2021-10-15 | 长江大学 | Method for quantitatively predicting erosion and pore volume increase of clastic rock reservoir based on process response |
| CN108733985A (en) * | 2018-06-08 | 2018-11-02 | 中国科学院城市环境研究所 | A kind of method of the determining critical environments parameter for restricting microalgae arsenic accumulation capability |
| CN108733985B (en) * | 2018-06-08 | 2021-06-25 | 中国科学院城市环境研究所 | Method for determining key environmental parameters for restricting arsenic accumulation capacity of microalgae |
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| CN103173520B (en) | 2014-06-04 |
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