CN103173520B - Bioerosion action method of quantitative microalgae to limestone - Google Patents
Bioerosion action method of quantitative microalgae to limestone Download PDFInfo
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Abstract
The invention discloses a bioerosion action method of quantitative microalgae to limestone. One part of magnesium ions which are dissolved out of limestone are absorbed and utilized by the algae, and one part can cause the changes in concentration of the magnesium ions in a solution; the integration of a microalgae growth equation to time represents the bioerosion accumulative action time of the algae in unit volume of culture solution; an equation of the dissolving-out quantity of the microalgae in the unit volume of the culture solution to limestone magnesium along with the changes in bioerosion accumulative action time of the algae represents the dissolving-out quantity of the microalgae in the unit volume of the culture solution to limestone magnesium under different bioerosion accumulative action times of the algae, and then derivation is performed on the equation to represent the dissolving-out quantity of algae in unit biomass unit time to limestone magnesium; and the dissolving-out of magnesium can also directly reflect the erosion of limestone, so that the dissolving-out quantity of the algae in the unit biomass unit time to limestone magnesium can quantitatively reflect the bioerosion action of the microalgae to limestone. The bioerosion action method disclosed by the invention can fill in the blank of bioerosion action of the quantitative microalgae to limestone and has the advantages of mature technology, simplicity in operation and low cost.
Description
Technical field
The present invention relates to the bioerodable action method of a kind of quantitative micro-algae to limestone, belong to ecological environment treatment monitoring and engineering geology monitoring field.
Background technology
The corrosion of limestone, comprises physical action, chemical action and biological action.Limestone often suffers biological corrosion around, and therefore, bioerodable effect is most important.Karst region rock surface has abundant algae to assemble, and in water body, a large amount of algae corrode the rock in substrate and embankment.
?although the micro-form of biokarst and retentiveness that someone forms the corrosion of the shallow top layer of limestone algae, lichens, liver moss were carried out research, the effect in karstification process is studied to microorganism and carbonic anhydrase also people.But the quantitative examination to " the biological bioerodable effect to limestone " this complex process have not been reported.
Micro-algae (microalgae) comprises the swim microphyte of mode of life of all Shui Zhongying of living in, and conventionally just refers to planktonic algae.Micro-algal structure is simple, and its physiological process is also relatively simple, and some kind is the model plant of scientific research, as: Chlamydomonas reinhardtii, chlorella, all right artificial culture of many types, this research that is us is provided convenience.Micro-algae equally also comprises physical action, chemical action and biological action to Limestone Dissolution effect.Different algae is obviously different to the bioerodable effect of limestone, has the algae of the outer carbonic anhydrase of born of the same parents to have stronger corrosion effect compared with algae without the outer carbonic anhydrase of born of the same parents.At present, the corrosion effect of the micro-algae of different sorts is quantitatively a very stubborn problem.
?can produce a large amount of calcium ions and magnesium ions in micro-algae in to the bioerodable mechanism of limestone, these calcium ions and magnesium ions parts absorb for algae, and a part exists in solution, and some precipitates in sedimentary mode.Due to, the calcium magnesium mass ratio of limestone is generally greater than 40:1, and the calcium magnesium mass ratio of frond utilization is less than 10:1, and therefore, magnesium ion becomes the limiting factor of micro algae growth.In the time that algae utilizes the magnesium of stripping from limestone, although some calcium is also utilized by algae, but overwhelming majority's calcium of stripping from limestone is not utilized, or exist in solution with ionic species, or be deposited in solution with precipitation of calcium carbonate form, and the calcium carbonate quantity precipitating calculation more difficult to estimate, the impact that magnesium is precipitated with respect to calcium is less.Therefore, we can treat by mensuration the change in concentration of the magnesium in content and the solution of magnesium in micrometer algae, by the bioerodable effect of the series of computation unit of obtaining frond unit time to limestone.
Quantitative micro-algae have not been reported the method for limestone bioerodable effect at present, and quantitatively very important to the effect of limestone bioerodable of micro-algae, it can assess the harm of the buildings such as river, lake Wei Zaodui karst region ecotope and dykes and dams effectively, thereby reaches the object of prediction disaster.Particularly current, in the serious situation of numerous lake eutrophications, seem even more important and urgent.
Summary of the invention
The technical problem to be solved in the present invention is, the quantivative approach of a kind of micro-algae to the effect of limestone bioerodable is provided, and fills up the blank of the bioerodable effect of quantitative micro-algae to limestone.
?the present invention takes following technical scheme: the bioerodable action method of a kind of quantitative micro-algae to limestone, is characterized in that: comprise following steps:
First, measure limestone powder calcium magnesium mass ratio, after adding respectively excessive calcium magnesium mass ratio be greater than 40 limestone powder and do not add limestone powder in two groups of cellar culture liquid, cultivate and treat micrometer algae, then in another group nutrient solution, only add this calcium magnesium mass ratio and be greater than 40 limestone powder and do not add frond;
Second, treat that micrometer algae cultivates under the culture condition of being investigated, after different incubation times, measure respectively and be added with limestone powder and cultivation and have the chlorophyll-a concentration of the nutrient solution of frond, the magnesium ion concentration of corresponding incubation time solution and only add limestone powder and do not cultivate the magnesium ion concentration of solution in the nutrient solution that has frond, carrying out normalization method calibration with the magnesium ion concentration of solution that only adds limestone powder and do not cultivate the initial magnesium ion concentration of solution in the nutrient solution of frond and its different time is added with limestone powder and cultivation and has the magnesium ion concentration of solution in the nutrient solution of corresponding incubation time of frond, be added with limestone powder and the cultivation that obtain after the calibration of corresponding incubation time have magnesium ion concentration in the nutrient solution of frond, there are magnesium ion concentration data in the nutrient solution of frond to deduct the magnesium ion concentration of its initial solution be added with limestone powder and cultivation after the calibration of different incubation times again, show that under different incubation times, being added with limestone powder and cultivation has the changing value of magnesium ion concentration in the nutrient solution of frond,
The 3rd, to wait to cultivate more than 6 days, results, not adding the frond of cultivating in limestone powder nutrient solution, are measured the content of this frond magnesium and corresponding Chlorophyll-a Content, and Mg content corresponding to Units of Account quality chlorophyll a;
The 4th, after the chlorophyll-a concentration value that is added with limestone powder and cultivation under different incubation times and has the nutrient solution of frond is deducted to its initial chlorophyll-a concentration value, be multiplied by the Mg content that unit mass chlorophyll a is corresponding, obtain the magnesium amount from limestone stripping that under different incubation times, unit volume nutrient solution frond absorbs;
The 5th, the changing value that is added with limestone powder and cultivation under different incubation times and has magnesium ion concentration in the nutrient solution of frond is added to the magnesium amount from limestone stripping that under corresponding incubation time, unit volume nutrient solution frond absorbs, obtain the stripping quantity of different incubation time unit volume nutrient solution fronds to limestone magnesium;
The 6th, there are the nutrient solution chlorophyll-a concentration data of frond according to being added with limestone powder and cultivation under different incubation times, build the exponential growth equation that the chlorophyll-a concentration of micro-algae under culture condition to be measured changes with incubation time, be micro-algae Growth Equation;
The 7th, micro-algae Growth Equation, to time integral, is obtained to unit volume nutrient solution micro-algae bioerodable cumulative function equation of time, and calculate under different incubation times micro-algae bioerodable cumulative function time in unit volume nutrient solution;
The 8th, build the equation that in unit volume nutrient solution, different incubation time fronds change with micro-algae bioerodable cumulative function time of correspondence the stripping quantity of limestone magnesium, and to this equation differentiate, draw frond to be measured under the unit time unit's Chlorophyll-a Content bioerodable effect to limestone;
The 9th, micro-algae of unit time unit's Chlorophyll-a Content is carried out to the conversion of corrosion amount to the bioerodable effect of limestone, the corrosion amount of micro-algae of acquisition unit time unit Chlorophyll-a Content to limestone; Micro-algae that concrete conversion method is unit time unit's Chlorophyll-a Content is multiplied by corrosion amount conversion factor F to the bioerodable effect of limestone, and corrosion amount conversion factor F represents with following corrosion amount conversion equation:
B in above-mentioned corrosion amount conversion equation
mgand B
cabe respectively experiment limestone magnesium used and the shared mass ratio of calcium; M
mgCO3and M
caCO3be respectively the molecular mass of magnesiumcarbonate and calcium carbonate; M
mgand M
cabe respectively the molecular mass of magnesium and calcium.
advantage of the present invention is as follows:
The quantitatively principle of the bioerodable effect of micro-algae of unit frond unit time to limestone:
A magnesium part for limestone stripping absorbs for frond, a part causes the magnesium ion concentration in solution to change, the stripping quantity of limestone magnesium corresponding to frond bioerodable cumulative function time in unit volume nutrient solution, is representing in unit volume nutrient solution the corrosion situation of limestone under the total cumulative time of frond bioerodable effect.Therefore, can utilize the derivative of frond bioerodable cumulative function time in unit volume nutrient solution and the corresponding micro-algae stripping quantity equation to limestone magnesium, the bioerodable effect of micro-algae of the unit's of acquisition frond unit time to limestone.
?a magnesium ion part for limestone stripping absorbs for frond, a part causes the magnesium ion concentration in solution to change, and therefore the magnesium ion concentration changing value of different incubation time solution adds that the unit volume nutrient solution frond magnesium ion amount of absorbing is representing the stripping quantity of micro-algae to limestone magnesium in unit volume nutrient solution.With micro-algae Growth Equation, time integral is being represented to the frond bioerodable cumulative function time in unit volume nutrient solution.The equation that the micro-algae of unit volume nutrient solution changes with the frond bioerodable cumulative function time the stripping quantity of limestone magnesium is representing under the different frond bioerodable cumulative function time stripping quantity of micro-algae to limestone magnesium in unit volume nutrient solution, then its differentiate has been represented to the stripping quantity of unit biomass unit time frond to limestone magnesium.And the stripping of magnesium has directly reflected the corrosion of limestone, therefore micro-algae can be reflected the bioerodable effect of micro-algae to limestone quantitatively to the stripping quantity of limestone magnesium the unit biomass unit time.
?advantage of the present invention is as follows:
1) the bioerodable effect of the quantitative micro-algae of present method energy to limestone, has filled up the blank of quantitative micro-algae to the effect of limestone bioerodable;
2) present method only need to be measured chlorophyll-a concentration and Mg content, technology maturation, and simple to operate, cost is low;
3) present method is carried out culture experiment under identical experiment condition, and therefore, the data of obtaining the bioerodable effect of micro-algae to limestone are more reliable.
Embodiment
Embodiments of the invention: first step, measure the calcium magnesium mass ratio that will add limestone powder, after adding respectively excessive calcium magnesium mass ratio be greater than 40 limestone powder (being less than 60 orders) and do not add limestone powder in two groups of cellar culture liquid, cultivate and treat micrometer algae, then in another group nutrient solution, only add this calcium magnesium mass ratio and be greater than 40 limestone powder and do not add frond;
Second step, treats that micrometer algae cultivates under the culture condition of being investigated, and after different incubation times, measures respectively and is added with limestone powder and cultivation and has the chlorophyll-a concentration CHLa of the nutrient solution of frond
i, corresponding incubation time solution magnesium ion concentration CMg
ionly add limestone powder and do not cultivate the magnesium ion concentration CO of solution in the nutrient solution that has frond
i, do not cultivate the initial magnesium ion concentration CO of solution in the nutrient solution that has frond with only adding limestone powder
0solution magnesium ion concentration CO with its different time
icarrying out normalization method calibration is added with limestone powder and cultivation and has the magnesium ion concentration CMg of solution in the nutrient solution of corresponding incubation time of frond
i, be added with limestone powder and the cultivation that obtain after the calibration of corresponding incubation time have magnesium ion concentration CTMg in the nutrient solution of frond
i, calibration equation is CTMg
i=CMg
icO
0/ CO
i.Again be added with limestone powder and cultivation after the calibration of different incubation times are had to magnesium ion concentration data CTMg in the nutrient solution of frond
ideduct the magnesium ion concentration CTMg of its initial solution
0, show that under different incubation times, being added with limestone powder and cultivation has the changing value δ CTMg of magnesium ion concentration in the nutrient solution of frond
i;
Third step, waits to cultivate more than 6 days, and results, not adding the frond of cultivating in limestone powder nutrient solution, are measured the content of this frond magnesium and corresponding Chlorophyll-a Content, and Mg content CMgPChl corresponding to Units of Account quality chlorophyll a;
The 4th step, has the chlorophyll-a concentration value CHLa of the nutrient solution of frond by being added with limestone powder and cultivation under different incubation times
ideduct its initial chlorophyll-a concentration value CHLa
0after be multiplied by the Mg content CMgPChl that unit mass chlorophyll a is corresponding, obtain the magnesium amount Qa from limestone stripping that under different incubation times, unit volume nutrient solution frond absorbs
i;
The 5th step, will be added with limestone powder and cultivation and have the changing value δ CTMg of magnesium ion concentration in the nutrient solution of frond under different incubation times
iadd the magnesium amount Qa from limestone stripping that under corresponding incubation time, unit volume nutrient solution frond absorbs
i, obtain the stripping quantity Q of different incubation time unit volume nutrient solution fronds to limestone magnesium
i;
The 6th step, has the nutrient solution chlorophyll-a concentration CHLa of frond according to being added with limestone powder and cultivation under different incubation times
idata, build the chlorophyll-a concentration of micro-algae under culture condition to be measured with incubation time T change exponential growth equation, micro-algae Growth Equation;
The 7th step, to time integral, obtains unit volume nutrient solution micro-algae bioerodable cumulative function equation of time by micro-algae Growth Equation, and calculates under different incubation times micro-algae bioerodable cumulative function time PT in unit volume nutrient solution
i;
The 8th step, builds the stripping quantity Q of the different incubation time fronds of unit volume nutrient solution to limestone magnesium
iwith micro-algae bioerodable cumulative function time PT of correspondence
ithe equation changing, and to this equation differentiate, is multiplied by the unit conversion factor by derivative, carries out unit conversion, draws frond to be measured under the unit time unit's Chlorophyll-a Content bioerodable effect E to limestone;
The 9th step. micro-algae of unit time unit's Chlorophyll-a Content is carried out to the conversion of corrosion amount to the bioerodable effect E of limestone, obtain the corrosion amount M of the micro-algae of unit time unit's Chlorophyll-a Content to limestone; Micro-algae that concrete conversion method is unit time unit's Chlorophyll-a Content is multiplied by corrosion amount conversion factor F to the bioerodable effect E of limestone; Corrosion amount conversion factor F represents with following corrosion amount conversion equation:
B in above-mentioned corrosion amount conversion equation
mgand B
cabe respectively experiment limestone magnesium used and the shared mass ratio of calcium; M
mgCO3and M
caCO3be respectively the molecular mass of magnesiumcarbonate and calcium carbonate; M
mgand M
cabe respectively the molecular mass of magnesium and calcium.
chlamydomonas is measured the bioerodable effect of limestone:
Cultivated material is: chlamydomonas.Basic culture solution adopts SE substratum (not adding soil extract), and basic culture condition is: photoperiod L/D:12h/12h; 25 ℃ of temperature; Intensity of illumination is 100
, pH value 7.0(regulates with hydrochloric acid and sodium hydroxide).The limestone powder (being less than 60 orders) that to add respectively calcium magnesium mass ratio that 1g measured in two groups of nutrient solutions be 90:1 and do not add limestone powder after cultivate chlamydomonas to be measured, then in another group nutrient solution, only add same this limestone powder 1g and do not add chlamydomonas.Cultivate after 0,1,3,5,7,9 day until chlamydomonas, measure respectively the nutrient solution Determination of Chlorophyll a concentration C HLa that is added with limestone powder
ithe magnesium ion concentration CMg of (table 1), solution
i(table 2) and only add the magnesium ion concentration CO of solution in the nutrient solution of limestone powder
i, and with the initial magnesium ion concentration CO that only adds solution in limestone powder nutrient solution
0magnesium ion concentration CO with the solution of its different time
icarrying out normalization method calibration is added with limestone powder and cultivation and has the magnesium ion concentration CMg of solution in the nutrient solution of corresponding incubation time of chlamydomonas
i, be added with limestone powder and the cultivation that obtain after the calibration of corresponding incubation time have magnesium ion concentration CTMg in the nutrient solution of chlamydomonas
i(table 2), calibration equation is CTMg
i=CMg
icO
0/ CO
i.Again be added with limestone powder and cultivation after the calibration of different incubation times are had to magnesium ion concentration data CTMg in the nutrient solution of chlamydomonas
ideduct the magnesium ion concentration CTMg of its starting soln
0, show that under different incubation times, being added with limestone powder and cultivation has the changing value δ CTMg of magnesium ion concentration in the nutrient solution of chlamydomonas
i(table 3).Results are cultivated after 9 days and are not added the chlamydomonas of cultivating in limestone powder nutrient solution, measure its Mg content and Chlorophyll-a Content, and Mg content CMgPChl corresponding to Units of Account quality chlorophyll a.There is the chlorophyll-a concentration value CHLa of the nutrient solution of chlamydomonas by being added with limestone powder and cultivation under different incubation times
ideduct its initial chlorophyll-a concentration value CHLa
0after be multiplied by the Mg content CMgPChl that unit mass chlorophyll a is corresponding, obtain the magnesium amount Qa from limestone stripping that under different incubation times, unit volume nutrient solution chlamydomonas absorbs
i(table 3).
?to under different incubation times, be added with limestone powder and cultivation and have the changing value δ CTMg of magnesium ion concentration in the nutrient solution of chlamydomonas
iadd the magnesium amount Qa from limestone stripping that under corresponding incubation time, unit volume nutrient solution chlamydomonas absorbs
i, obtain the stripping quantity Q of different incubation time unit volume nutrient solution chlamydomonas to limestone magnesium
i(table 3).
?building the chlorophyll-a concentration of chlamydomonas under the culture condition to be measured exponential growth equation that T changes in time, is chlamydomonas Growth Equation, and chlamydomonas Growth Equation, to time integral, is obtained to unit volume nutrient solution chlamydomonas bioerodable cumulative function equation of time PT
i(table 4).
According to unit volume nutrient solution chlamydomonas bioerodable cumulative function equation of time, calculate under different incubation times chlamydomonas bioerodable cumulative function time PT in unit volume nutrient solution
i, as table (5).
Construct straight-line equation by table 5: Q
i=2.38 × 10
-3+ 3.01 × 10
-4pT
i(R
2=1, P<0.0001), this equation differentiate is obtained to slope 3.01 × 10
-4(unit:
), slope is multiplied by the unit conversion factor 3.6 × 10
5, carry out unit conversion, obtain E=108.36(unit:
), be that the chlamydomonas of unit time unit's Chlorophyll-a Content is to the bioerodable effect E of limestone.Again the chlamydomonas of unit time unit's Chlorophyll-a Content is multiplied by corrosion amount conversion factor F to the bioerodable effect E of limestone.Corrosion amount conversion factor F represents with following corrosion amount conversion equation:
B in above-mentioned corrosion amount conversion equation
mgand B
cabe respectively experiment limestone magnesium used and the shared mass ratio of calcium; M
mgCO3and M
caCO3be respectively the molecular mass of magnesiumcarbonate and calcium carbonate; M
mgand M
cabe respectively the molecular mass of magnesium and calcium.
?f is 228.50, and therefore, the chlamydomonas that contains every year 1g Chlorophyll-a Content is 24760.26(unit to the corrosion amount M of limestone:
), the chlamydomonas that also contains 1g Chlorophyll-a Content can corrosion in year the limestone of 24760.26 grams.
the mensuration of the bioerodable effect of chlorella to limestone:
Cultivated material is: chlorella.Basic culture solution adopts SE substratum (not adding soil extract), and basic culture condition is: photoperiod L/D:12h/12h; 25 ℃ of temperature; Intensity of illumination is 100
, pH value 7.0(regulates with hydrochloric acid and sodium hydroxide).The limestone powder (being less than 60 orders) that to add respectively calcium magnesium mass ratio that 1g measured in two groups of nutrient solutions be 90:1 and do not add limestone powder after cultivate chlorella to be measured, then in another group nutrient solution, only add same this limestone powder 1g and do not add chlorella.Cultivate after 0,1,3,5,7,9 day until chlorella, measure respectively the nutrient solution Determination of Chlorophyll a concentration C HLa that is added with limestone powder
ithe magnesium ion concentration CMg of (table 6), solution
i(table 7) and only add the magnesium ion concentration CO of solution in the nutrient solution of limestone powder
i, and with the initial magnesium ion concentration CO of solution only adding in limestone powder nutrient solution
0magnesium ion concentration CO with the solution of its different time
icarrying out normalization method calibration is added with limestone powder and cultivation and has the magnesium ion concentration CMg of solution in the nutrient solution of corresponding incubation time of chlorella
i, be added with limestone powder and the cultivation that obtain after the calibration of corresponding incubation time have CTMg in the nutrient solution of chlorella
i(table 7), calibration equation is CTMg
i=CMg
icO
0/ CO
i.To after the calibration of different incubation times, be added with limestone powder and cultivation and have magnesium ion concentration data CTMg in the nutrient solution of chlorella again
ideduct the magnesium ion concentration CTMg of its initial solution
0, show that under different incubation times, being added with limestone powder and cultivation has the changing value δ CTMg of magnesium ion concentration in the nutrient solution of chlorella
i(table 8).Results are cultivated after 9 days and are not added the chlorella of cultivating in limestone powder nutrient solution, measure its Mg content and Chlorophyll-a Content, and Mg content CMgPChl corresponding to Units of Account quality chlorophyll a.There is the chlorophyll-a concentration value CHLa of the nutrient solution of chlorella by being added with limestone powder and cultivation under different incubation times
ideduct its initial chlorophyll-a concentration value CHLa
0after be multiplied by the Mg content CMgPChl that unit mass chlorophyll a is corresponding, obtain the magnesium amount Qa from limestone stripping that under different incubation times, unit volume nutrient solution chlorella absorbs
i(table 8).
?to under different incubation times, be added with limestone powder and cultivation and have the changing value δ CTMg of magnesium ion concentration in the nutrient solution of chlorella
iadd the magnesium amount Qa from limestone stripping that under corresponding incubation time, unit volume nutrient solution chlorella absorbs
i, obtain the stripping quantity Q of chlorella to limestone magnesium in different incubation time unit volume nutrient solutions
i(table 8).
Build the chlorophyll-a concentration of chlorella under the culture condition to be measured exponential growth equation that T changes in time, it is chlorella Growth Equation, chlorella Growth Equation, to time integral, is obtained to unit volume nutrient solution chlorella bioerodable cumulative function equation of time (table 9).
According to unit volume nutrient solution chlorella bioerodable cumulative function equation of time, calculate under different incubation times chlorella bioerodable cumulative function time PT in unit volume nutrient solution
i, as table (10).
?construct straight-line equation by table 10: Q
i=0.23+2.16 × 10
-4pT
i(R
2=0.98, P=0.0008), this equation differentiate is obtained to slope 2.16 × 10
-4(unit:
), slope is multiplied by the unit conversion factor 3.6 × 10
5, carry out unit conversion, obtain E=77.76(unit:
), be that the chlorella of unit time unit's Chlorophyll-a Content is to the bioerodable effect E of limestone.Again the chlorella of unit time unit's Chlorophyll-a Content is multiplied by corrosion amount conversion factor F to the bioerodable effect E of limestone.Corrosion amount conversion factor F represents with following corrosion amount conversion equation:
B in above-mentioned corrosion amount conversion equation
mgand B
cabe respectively experiment limestone magnesium used and the shared mass ratio of calcium; M
mgCO3and M
caCO3be respectively the molecular mass of magnesiumcarbonate and calcium carbonate; M
mgand M
cabe respectively the molecular mass of magnesium and calcium.
?f is 228.50, and therefore, the chlorella that contains every year 1g Chlorophyll-a Content is 17768.16(unit to the corrosion amount M of limestone:
), the chlorella that also contains 1g Chlorophyll-a Content can corrosion in year the limestone of 17768.16 grams.
effect:
In summary it can be seen, chlamydomonas is greater than the bioerodable effect of chlorella to limestone to the bioerodable effect of limestone, the outer carbonic anhydrase activity of born of the same parents of this and chlamydomonas is greater than chlorella, thereby and carbonic anhydrase can promote the carrying out of hydrated carbon dioxide reversible reaction to promote the fact of the corrosion of limestone to conform to.In addition, the chlamydomonas that contains 1g chlorophyll a can corrosion in year limestone and the chlorella that contains 1g chlorophyll a of 24760.26 grams can corrosion in year the experimental result of limestone of 17768.16 grams also tally with the actual situation, show that utilizing micro-algae that the present invention obtains is believable to limestone bioerodable effects of action.
Claims (1)
1. the bioerodable action method of quantitative micro-algae to limestone, is characterized in that: comprise following steps:
First, measure limestone powder calcium magnesium mass ratio, after adding respectively excessive calcium magnesium mass ratio be greater than 40 limestone powder and do not add limestone powder in two groups of cellar culture liquid, cultivate and treat micrometer algae, then in another group nutrient solution, only add this calcium magnesium mass ratio and be greater than 40 limestone powder and do not add frond;
The second, treat that micrometer algae cultivates under the culture condition of being investigated, after different incubation times, measure respectively and be added with limestone powder and cultivation has the chlorophyll-a concentration of the nutrient solution of frond, the magnesium ion concentration CMg of corresponding incubation time solution
ionly add limestone powder and do not cultivate the magnesium ion concentration CO of solution in the nutrient solution that has frond
i, do not cultivate the initial magnesium ion concentration CO of solution in the nutrient solution that has frond with only adding limestone powder
0solution magnesium ion concentration CO with its different time
icarrying out normalization method calibration is added with limestone powder and cultivation and has the magnesium ion concentration CMg of solution in the nutrient solution of corresponding incubation time of frond
i, be added with limestone powder and the cultivation that obtain after the calibration of corresponding incubation time have magnesium ion concentration CTMg in the nutrient solution of frond
i, calibration equation is CTMg
i=CMg
icO
0/ CO
i, then be added with limestone powder and cultivation after the calibration of different incubation times are had to magnesium ion concentration data CTMg in the nutrient solution of frond
ideduct the magnesium ion concentration CTMg of its initial solution
0, show that under different incubation times, being added with limestone powder and cultivation has the changing value δ CTMg of magnesium ion concentration in the nutrient solution of frond
i, i.e. δ CTMg
i=CTMg
icTMg
0;
The 3rd, to wait to cultivate more than 6 days, results, not adding the frond of cultivating in limestone powder nutrient solution, are measured the content of this frond magnesium and corresponding Chlorophyll-a Content, and Mg content corresponding to Units of Account quality chlorophyll a;
The 4th, after the chlorophyll-a concentration value that is added with limestone powder and cultivation under different incubation times and has the nutrient solution of frond is deducted to its initial chlorophyll-a concentration value, be multiplied by the Mg content that unit mass chlorophyll a is corresponding, obtain the magnesium amount from limestone stripping that under different incubation times, unit volume nutrient solution frond absorbs;
The 5th, the changing value that is added with limestone powder and cultivation under different incubation times and has magnesium ion concentration in the nutrient solution of frond is added to the magnesium amount from limestone stripping that under corresponding incubation time, unit volume nutrient solution frond absorbs, obtain the stripping quantity of different incubation time unit volume nutrient solution fronds to limestone magnesium;
The 6th, there are the nutrient solution chlorophyll-a concentration data of frond according to being added with limestone powder and cultivation under different incubation times, build the exponential growth equation that the chlorophyll-a concentration of micro-algae under culture condition to be measured changes with incubation time, be micro-algae Growth Equation;
The 7th, micro-algae Growth Equation, to time integral, is obtained to unit volume nutrient solution micro-algae bioerodable cumulative function equation of time, and calculate under different incubation times micro-algae bioerodable cumulative function time in unit volume nutrient solution;
The 8th, build the equation that in unit volume nutrient solution, different incubation time fronds change with micro-algae bioerodable cumulative function time of correspondence the stripping quantity of limestone magnesium, and to this equation differentiate, draw frond to be measured under the unit time unit's Chlorophyll-a Content bioerodable effect to limestone;
The 9th, micro-algae of unit time unit's Chlorophyll-a Content is carried out to the conversion of corrosion amount to the bioerodable effect of limestone, the corrosion amount of micro-algae of acquisition unit time unit Chlorophyll-a Content to limestone; Micro-algae that concrete conversion method is unit time unit's Chlorophyll-a Content is multiplied by corrosion amount conversion factor F to the bioerodable effect of limestone, and corrosion amount conversion factor F represents with following corrosion amount conversion equation:
F=
B in above-mentioned corrosion amount conversion equation
mgand B
cabe respectively experiment limestone magnesium used and the shared mass ratio of calcium; M
mgCO3and M
caCO3be respectively the molecular mass of magnesiumcarbonate and calcium carbonate; M
mgand M
cabe respectively the molecular mass of magnesium and calcium.
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| 微生物对碳酸盐岩的风化作用;连宾等;《地学前缘》;20081115(第06期);全文 * |
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