CN105594745A - Ustilaginoidea virens inhibitor prepared from sorbose - Google Patents

Ustilaginoidea virens inhibitor prepared from sorbose Download PDF

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Publication number
CN105594745A
CN105594745A CN201610078106.2A CN201610078106A CN105594745A CN 105594745 A CN105594745 A CN 105594745A CN 201610078106 A CN201610078106 A CN 201610078106A CN 105594745 A CN105594745 A CN 105594745A
Authority
CN
China
Prior art keywords
sorbose
ustilaginoidea virens
mortifier
ustilaginoidea
virens
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201610078106.2A
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Chinese (zh)
Inventor
张君成
王忠文
曾东强
杨平
蒲相君
时婷婷
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Guangxi University
Original Assignee
Guangxi University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guangxi University filed Critical Guangxi University
Priority to CN201610078106.2A priority Critical patent/CN105594745A/en
Publication of CN105594745A publication Critical patent/CN105594745A/en
Pending legal-status Critical Current

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Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N61/00Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom

Abstract

The invention discloses an ustilaginoidea virens inhibitor prepared from sorbose. The inhibitor is prepared from the compound sorbose and is produced through carrying out high-temperature treatment on the sorbose. The ustilaginoidea virens inhibitor prepared from the sorbose, disclosed by the invention, has the beneficial effects that the effect of inhibiting the growth and development of ustilaginoidea virens is strong. The inhibitor can provide effective and feasible technical beginning and technical approach for researching ustilaginoidea virens prevention and treatment drugs.

Description

A kind of ustilaginoidea virens mortifier that comes from sorbose
Technical field
The present invention relates to plant pathology learns a skill and Pesticide Science technology. Specifically a kind of rice song that comes from sorboseGerm mortifier.
Technical background
Infect and cause by ustilaginoidea virens (Ustilaginoideavirens (Cooke) Takahashi)The false smut whole world occurs, and belongs to the Major Diseases of China's Rice Production, and in some rice districts, false smut is even superCross rice blast and become the great disease of Rice Production. Be limited to and lack enough to the rule of causing disaster of plant diseaseUnderstanding, current and from now on quite over a long time in, the control of plant disease still mainly depends on medicine (agricultural chemicals)Control, the control of false smut is also like this. Therefore, the research and development of false smut control medicine just become solutionThe certainly important technology work of false smut problem.
In history, false smut one is directly subordinate to the lighter Minor diseases of causing harm, and is that the swift and violent of nearly more than ten years sent outOpen up, just rise to the great disease of Rice Production. Owing to failing in history to obtain enough attention, false smutThe related basic study of control is still weak, lags behind especially for the medicament research and development of false smut control. In fact,Be limited to the biological shallow understanding of ustilaginoidea virens, current medicament research and development of preventing and treating false smut is almost confined to cityDirectly using or existing medicine composite usage of face agricultural chemicals, the research and development of the source medicine of relevant false smut control are non-Chang Xianjian. In view of the significant impact of false smut to rice grain safety, strengthen false smut to prevent and treat medicament research and development obviousVery necessary. Technical, fully excavate and find the inhibiting substances that ustilaginoidea virens grows, not only favourableIn the lead drug of finding to prevent and treat false smut, also the approach of false smut control drug world research and development will be widened.
Summary of the invention
The present invention seeks to, a kind of ustilaginoidea virens mortifier that comes from sorbose is provided.
The technical scheme that the present invention solves the problems of the technologies described above is as follows:
The ustilaginoidea virens mortifier that derives from sorbose derives from a compound sorbose, by sorbose through heightTemperature generates after processing. This mortifier has effect that strong inhibition ustilaginoidea virens grows. This mortifier energyFor effective and feasible technology beginning and the technological approaches of research and development false smut control drug provision.
Beneficial effect of the present invention:
A kind of ustilaginoidea virens mortifier that derives from sorbose of the present invention can be carried for research and development false smut control medicineFor effective and feasible technology beginning and technological approaches.
Brief description of the drawings
Fig. 1 is that the present invention contrasts CK to ustilaginoidea virens inhibition effect inspection figure.
Fig. 2 is that the efficient mortifier of the present invention suppresses effect inspection figure to ustilaginoidea virens.
Detailed description of the invention
Below in conjunction with accompanying drawing and embodiment, the invention will be further described.
Inventor finds in false smut research, is containing in the conventional medium of sorbose (1%) false smutBacterium can not normal growth grows, and is once once thinking that the growth that compound sorbose can suppress ustilaginoidea virens sent out by mistakeEducate. Up-to-date test is found, in the culture medium that is background, adds at the potato agar that is conducive to ustilaginoidea virens growthAdd sorbose (content is 1%), when the sorbose adding does not pass through high-temperature process, ustilaginoidea virens is in this trainingSupport on base and can grow by normal growth, as shown in Figure 1; And when same sorbose is through high-temperature process, rice songThe normal growth of germ on this culture medium grown and is subject to strong inhibition, as shown in Figure 2. Obviously, compoundSorbose itself is harmless to ustilaginoidea virens, but the biological effectiveness of the high-temperature process product of sorbose occursThe variation of matter, become suppress ustilaginoidea virens normal growth grow, namely sorbose after high-temperature process,Produced the material that ustilaginoidea virens is played to strong inhibition effect, such result of study yet there are no openPrecedent. The strong bacteriostasis efficacy of this inhibiting substances to ustilaginoidea virens, has before the potentiality and application of exploitationScape, on the one hand, can directly research and develop by the control medicine using this mortifier as false smut, or verifyThe structure of this mortifier as guide's material, develops the similar and effect that inhibitory action is stronger of structureThing; And on the other hand, by studying action target and the mechanism of action of this mortifier to ustilaginoidea virens, and thenExcavate and explore the more effective agent for these targets; Thereby the compound that derives from disclosed by the inventionThe mortifier of sorbose, can provide effective and feasible technology beginning and technology for the research and development that false smut is prevented and treated medicineApproach.
Embodiment
In the potato agar culture medium background of suitable ustilaginoidea virens growth, add the rice that derives from sorboseBent germ mortifier, can detect antibacterial to ustilaginoidea virens of the inhibiting substances that derives from sorbose easilyEffect. Concrete implementation and operation is as follows:
1. prepare the prewired culture medium P of potato agar
Adopt the potato agar culture medium that suitable ustilaginoidea virens grows to make background culture medium, background is cultivatedThe prewired ratio of base is: potato 200g, agar 40g, distilled water 1000ml. After conventional high-temperature sterilizationObtain the prewired culture medium P of potato agar.
2. prepare sorbose solution A and high-temperature process product B thereof
In proportion: sorbose 20g, distilled water 1000ml, be made into sorbose solution, use at normal temperatures bacteriumFilter, by this sorbose solution filter sterilizing, obtains the sorbose without high-temperature process but in sterilizing stateSolution A. This sorbose solution A is placed in common high pressure-temperature autoclave to 30 points of 126 DEG C of high-temperature processClock, the high-temperature process product B of cooling rear acquisition sorbose.
3. detect the bacteriostasis of mortifier
Under aseptic condition, by prewired potato agar culture medium P respectively with sorbose solution A, sorboseHigh-temperature process product B is carried out mixed in equal amounts, changes into culture plate, forms the identical 2 kinds of cultivations of backgroundBase: the high-temperature process product culture medium of A sorbose culture medium (comparing CK), B sorbose, and this 2Plant the proportioning the same (potato 100g, sorbose 10g, agar 20g, water 1000ml) of culture medium.
Ustilaginoidea virens is implanted into culture plate, and every dull and stereotyped spore liquid 9 points of implanting, are then placed in 28 DEG C of perseverancesIn temperature incubator, cultivate, within 5 days, take out and observe afterwards.
On A sorbose culture medium, grow 9 ustilaginoidea virens bacterium colonies clearly, as shown in Figure 1, obviouslyOn this culture medium, ustilaginoidea virens energy normal growth is grown.
And on the high-temperature process product culture medium of B sorbose, 9 spores are implanted point and are not found any growthSign, as shown in Figure 2, obviously this culture medium efficiently suppresses growing of ustilaginoidea virens. Very clear and definite, shouldIn culture medium, have the mortifier of ustilaginoidea virens, this mortifier efficiently suppresses growing of ustilaginoidea virens.
Comparison diagram 1 and Fig. 2, visible compound sorbose itself is harmless, inhibitory action is from sorboseHigh-temperature process product, sorbose decomposes and has generated the growth of efficient inhibition ustilaginoidea virens and send out after high-temperature processThe material of educating.
The ustilaginoidea virens mortifier that derives from sorbose disclosed by the invention, its inhibiting object is sent out at presentNow can suppress ustilaginoidea virens, according to general Biological Principles, probably this mortifier can also suppress some otherPathogen grows, and therefore, this mortifier also belongs to guarantor of the present invention to the inhibitory action of other pathogenProtect scope.

Claims (1)

1. a ustilaginoidea virens mortifier that derives from sorbose, is characterized in that, this mortifier derives from chemical combinationThing sorbose, is generated after high-temperature process by sorbose; This mortifier has the growth of strong inhibition ustilaginoidea virensThe effect of growing; This mortifier can be effective and feasible technology beginning and the skill of research and development false smut control drug provisionArt approach.
CN201610078106.2A 2016-02-03 2016-02-03 Ustilaginoidea virens inhibitor prepared from sorbose Pending CN105594745A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610078106.2A CN105594745A (en) 2016-02-03 2016-02-03 Ustilaginoidea virens inhibitor prepared from sorbose

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610078106.2A CN105594745A (en) 2016-02-03 2016-02-03 Ustilaginoidea virens inhibitor prepared from sorbose

Publications (1)

Publication Number Publication Date
CN105594745A true CN105594745A (en) 2016-05-25

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN86107277A (en) * 1985-10-22 1987-07-15 武田药品工业株式会社 Method for producing 2-keto-l-gulonic acid
CN1288456A (en) * 1998-01-19 2001-03-21 宝酒造株式会社 Substances capable of inducing appotosis
CN102453007A (en) * 2010-10-22 2012-05-16 王天任 Method for preparing 5-hydroxymethylfurfural
CN104357529A (en) * 2014-10-15 2015-02-18 沈阳药科大学 Method for improving production capacity of 2-KGA (2-keto-L-gulonic acid) through enhancement of Ketogulonogeniumvulgarum carbon metabolism level
US9125409B2 (en) * 2008-08-18 2015-09-08 Mitsui Chemicals Agro, Inc. Plant disease control agent comprising D-tagatose as active ingredient, and plant disease control method

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN86107277A (en) * 1985-10-22 1987-07-15 武田药品工业株式会社 Method for producing 2-keto-l-gulonic acid
CN1288456A (en) * 1998-01-19 2001-03-21 宝酒造株式会社 Substances capable of inducing appotosis
US9125409B2 (en) * 2008-08-18 2015-09-08 Mitsui Chemicals Agro, Inc. Plant disease control agent comprising D-tagatose as active ingredient, and plant disease control method
CN102453007A (en) * 2010-10-22 2012-05-16 王天任 Method for preparing 5-hydroxymethylfurfural
CN104357529A (en) * 2014-10-15 2015-02-18 沈阳药科大学 Method for improving production capacity of 2-KGA (2-keto-L-gulonic acid) through enhancement of Ketogulonogeniumvulgarum carbon metabolism level

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
张君成 等: "糖对稻曲病菌薄壁分生孢子萌发的影响", 《植物病理学报》 *
蔡磊 等: "高温液态水中果糖无催化分解反应动力学", 《化工学报》 *

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Application publication date: 20160525