CN105541927A - Ribose-derived Ustilaginoidea virens inhibitor - Google Patents
Ribose-derived Ustilaginoidea virens inhibitor Download PDFInfo
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- CN105541927A CN105541927A CN201610075808.5A CN201610075808A CN105541927A CN 105541927 A CN105541927 A CN 105541927A CN 201610075808 A CN201610075808 A CN 201610075808A CN 105541927 A CN105541927 A CN 105541927A
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- ribose
- ustilaginoidea virens
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07G—COMPOUNDS OF UNKNOWN CONSTITUTION
- C07G99/00—Subject matter not provided for in other groups of this subclass
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
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- Organic Chemistry (AREA)
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- Dentistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a ribose-derived Ustilaginoidea virens inhibitor which is derived from the compound ribose. The inhibitor is formed by carrying out high-temperature treatment on ribose. The ribose-derived Ustilaginoidea virens inhibitor has the effect of strongly inhibiting growth and development of Ustilaginoidea virens. The inhibitor can provide an effective feasible technical start and technical pathway for developing and researching drugs for preventing and treating Ustilaginoidea virens.
Description
Technical field
The present invention relates to plant pathology to learn a skill and Pesticide Science technology.Specifically a kind of ustilaginoidea virens inhibition coming from ribose.
Technical background
Infect by ustilaginoidea virens (Ustilaginoideavirens (Cooke) Takahashi) the false smut whole world caused to occur, belong to the Major Diseases of China's Rice Production, in some rice districts, false smut even exceedes rice blast and becomes the great disease of Rice Production.Be limited to and enough understanding lacked to the plague law of Plant diseases, current and from now on quite over a long time in, the control of Plant diseases still depends on medicine (agricultural chemicals) control, and the control of false smut is also like this.Therefore, the research and development of false smut protective agents just become the important technology work solving false smut problem.
In history, false smut one is directly subordinate to lighter Minor diseases of causing harm, and just the fast development of nearly more than ten years, just rises to the great disease of Rice Production.Owing to failing to obtain enough attention in history, the related basic study of false smut control is still weak, and the medicament research and development for false smut control is delayed especially.In fact, be limited to the biological shallow understanding of ustilaginoidea virens, current medicament research and development of preventing and treating false smut is almost confined to directly using of market agricultural chemicals or existing medicine composite usage, and the research and development of the source medicine of relevant false smut control are very rarely seen.In view of false smut is to the great effect of rice grain safety, the protective agents research and development strengthening false smut are obviously very necessary.Technically, fully excavate and find the inhibitory substance that ustilaginoidea virens grows, be not only conducive to the lead drug finding to prevent and treat false smut, also will widen the approach of false smut protective agents field research and development.
Summary of the invention
The present invention seeks to, a kind of ustilaginoidea virens inhibition coming from ribose is provided.
The technical scheme that the present invention solves the problems of the technologies described above is as follows:
The ustilaginoidea virens inhibition deriving from ribose derives from a compound ribose, is generated after pyroprocessing by ribose.This inhibition has effect that strongly inhibited ustilaginoidea virens grows.This inhibition can provide effective and feasible technology beginning and technological approaches for research and development false smut protective agents.
Benefit effect of the present invention:
A kind of ustilaginoidea virens inhibition deriving from ribose of the present invention can provide effective and feasible technology beginning and technological approaches for research and development false smut protective agents.
Accompanying drawing explanation
Fig. 1 is that the present invention contrasts CK to ustilaginoidea virens suppression power test figure.
Fig. 2 is that the efficient inhibition of the present invention suppresses power test figure to ustilaginoidea virens.
Embodiment
Below in conjunction with accompanying drawing and embodiment, the invention will be further described.
Contriver finds in false smut research, and in the conventional medium containing ribose (1%), ustilaginoidea virens can not be grown by normal growth, is once once thinking that compound ribose can suppress growing of ustilaginoidea virens by mistake.Up-to-date test finds, be conducive to ustilaginoidea virens growth potato agar be in the substratum of background, add ribose (content is 1%), when the ribose added is not through pyroprocessing, ustilaginoidea virens can be grown by normal growth on this substratum, as shown in Figure 1; And when same ribose is through pyroprocessing, the normal growth of ustilaginoidea virens on this substratum is grown and be subject to strongly inhibited, as shown in Figure 2.Obviously, compound ribose itself is harmless to ustilaginoidea virens, but the biological effectiveness of the pyroprocessing product of ribose there occurs the change of matter, become and suppress the normal growth of ustilaginoidea virens to be grown, namely ribose is after pyroprocessing, create material ustilaginoidea virens being played to strongly inhibited effect, such result of study yet there are no open precedent.This inhibitory substance is to the strong bacteriostasis efficacy of ustilaginoidea virens, there is potentiality and the application prospect of exploitation, on the one hand, the protective agents of this inhibition as false smut directly can be researched and developed, or verify the structure of this inhibition and as guide's material, develop similar and the stronger Substrate of restraining effect; And on the other hand, by studying this inhibition to the action target of ustilaginoidea virens and the mechanism of action, and then excavate and explore the more effective Substrate for these targets; Thus the inhibition deriving from compound ribose disclosed by the invention, can provide effective and feasible technology beginning and technological approaches for the research and development of false smut protective agents.
Embodiment
In the potato agar substratum background that suitable ustilaginoidea virens grows, add the ustilaginoidea virens inhibition deriving from ribose, can detect that the inhibitory substance deriving from ribose is to the bacteriostatic action of ustilaginoidea virens easily.Concrete operations are as follows:
1. prepare the prewired substratum P of potato agar
The potato agar substratum adopting suitable ustilaginoidea virens to grow makes background substratum, and the prewired ratio of background substratum is: potato 200g, agar 40g, distilled water 1000ml.The prewired substratum P of potato agar is obtained after conventional high temperature sterilizing.
2. prepare ribose solution A and pyroprocessing product B thereof
In proportion: ribose 20g, distilled water 1000ml, be made into ribose solution, at normal temperatures with biofilter by this ribose solution filtration sterilization, obtain without pyroprocessing but be in the ribose solution A of sterilizing state.This ribose solution A is placed in ordinary high pressure high-temperature sterilization pot, 126 DEG C of pyroprocessing 30 minutes, after cooling, obtains the pyroprocessing product B of ribose.
3. detect the bacteriostatic action of inhibition
Under aseptic condition, prewired for potato agar substratum P is carried out balanced mix with the pyroprocessing product B of ribose solution A, ribose respectively, change into culture plate, the identical 2 kinds of substratum of formation background: the pyroprocessing product free medium of A ribose substratum (comparing CK), B ribose, and the proportioning of these 2 kinds of substratum the same (potato 100g, ribose 10g, agar 20g, water 1000ml).
Ustilaginoidea virens is implanted into culture plate, and every dull and stereotyped implantation spore liquid 9 point, is then placed in 28 DEG C of constant incubators and cultivates, take out observation after 5 days.
On A ribose substratum, grow 9 ustilaginoidea virens bacterium colonies clearly, as shown in Figure 1, obviously on this substratum, ustilaginoidea virens energy normal growth is grown.
And on the pyroprocessing product free medium of B ribose, 9 spores are implanted point and only observed faint growth phenomenon, as shown in Figure 2, obviously this substratum efficiently suppresses growing of ustilaginoidea virens.Very clear and definite, there is the inhibition of ustilaginoidea virens in this substratum, this inhibition efficiently suppresses growing of ustilaginoidea virens.
Comparison diagram 1 and Fig. 2, visual compounds ribose itself is harmless, and restraining effect is from the pyroprocessing product of ribose, and namely ribose decomposes the material generating efficient suppression ustilaginoidea virens and grow after pyroprocessing.
The ustilaginoidea virens inhibition deriving from ribose disclosed by the invention; its inhibiting object finds to suppress ustilaginoidea virens at present; according to general Biological Principles; probably this inhibition can also suppress some other growth of pathogenic bacteria to be grown; therefore, the restraining effect of this inhibition to other pathogenic bacteria also belongs to protection scope of the present invention.
Claims (1)
1. derive from a ustilaginoidea virens inhibition for ribose, it is characterized in that, this inhibition derives from compound ribose, is generated after pyroprocessing by ribose; This inhibition has effect that strongly inhibited ustilaginoidea virens grows; This inhibition can provide effective and feasible technology beginning and technological approaches for research and development false smut protective agents.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610075808.5A CN105541927A (en) | 2016-02-03 | 2016-02-03 | Ribose-derived Ustilaginoidea virens inhibitor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610075808.5A CN105541927A (en) | 2016-02-03 | 2016-02-03 | Ribose-derived Ustilaginoidea virens inhibitor |
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| CN105541927A true CN105541927A (en) | 2016-05-04 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610075808.5A Pending CN105541927A (en) | 2016-02-03 | 2016-02-03 | Ribose-derived Ustilaginoidea virens inhibitor |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105779373A (en) * | 2016-05-24 | 2016-07-20 | 广西大学 | Ribose culture medium applicable to ustilaginoidea virens spore production and application method of ribose culture medium |
| CN106635948A (en) * | 2017-01-10 | 2017-05-10 | 广西大学 | Pollution-free preparing and culturing method for rice-false-smut-case thin-wall conidia |
Citations (4)
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|---|---|---|---|---|
| CN1230165A (en) * | 1996-09-27 | 1999-09-29 | 宝酒造株式会社 | Cyclopentenones, process for preparing same, and use thereof |
| CN1288456A (en) * | 1998-01-19 | 2001-03-21 | 宝酒造株式会社 | Substances capable of inducing appotosis |
| WO2002017902A1 (en) * | 2000-08-29 | 2002-03-07 | Takara Bio Inc. | Gene conferring resistance to the antibacterial 4,5-dihydroxy-2-cyclopenten-1-one (dhcp), the protein encoded by same, and applications thereof |
| CN1347317A (en) * | 1999-02-19 | 2002-05-01 | 宝酒造株式会社 | Remedies |
-
2016
- 2016-02-03 CN CN201610075808.5A patent/CN105541927A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1230165A (en) * | 1996-09-27 | 1999-09-29 | 宝酒造株式会社 | Cyclopentenones, process for preparing same, and use thereof |
| CN1288456A (en) * | 1998-01-19 | 2001-03-21 | 宝酒造株式会社 | Substances capable of inducing appotosis |
| CN1347317A (en) * | 1999-02-19 | 2002-05-01 | 宝酒造株式会社 | Remedies |
| WO2002017902A1 (en) * | 2000-08-29 | 2002-03-07 | Takara Bio Inc. | Gene conferring resistance to the antibacterial 4,5-dihydroxy-2-cyclopenten-1-one (dhcp), the protein encoded by same, and applications thereof |
Non-Patent Citations (1)
| Title |
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| 张君成，等: "糖对稻曲病菌薄壁分生孢子萌发的影响", 《植物病理学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105779373A (en) * | 2016-05-24 | 2016-07-20 | 广西大学 | Ribose culture medium applicable to ustilaginoidea virens spore production and application method of ribose culture medium |
| CN106635948A (en) * | 2017-01-10 | 2017-05-10 | 广西大学 | Pollution-free preparing and culturing method for rice-false-smut-case thin-wall conidia |
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