CN105311067A - Method for extracting total tribulus terrestris saponins from stems and leaves of tribulus terrestris - Google Patents
Method for extracting total tribulus terrestris saponins from stems and leaves of tribulus terrestris Download PDFInfo
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- CN105311067A CN105311067A CN201410364796.9A CN201410364796A CN105311067A CN 105311067 A CN105311067 A CN 105311067A CN 201410364796 A CN201410364796 A CN 201410364796A CN 105311067 A CN105311067 A CN 105311067A
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- tribulus terrestris
- fructus tribuli
- extract
- saponins
- stem
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Abstract
The invention discloses a method for extracting total tribulus terrestris saponins from stems and leaves of tribulus terrestris. The method includes the steps: (1) removing impurities such as dust from the stems and the leaves of the tribulus terrestris, and grinding the tribulus terrestris with impurities removed into 10-20-mesh powder by a hammer mill; (2) extracting ground raw materials according to a solvent method to obtain an extract, and subjecting the extract to concentration under reduced pressure to obtain a concentrate for standby application; (3) purifying and drying the concentrate to obtain the total tribulus terrestris saponins. The method is simple in process and high in content of extracted total tribulus terrestris saponins. Compared with a conventional method for extracting the total tribulus terrestris saponins from fruits, the method for extracting the total tribulus terrestris saponins from the stems and the leaves of the tribulus terrestris has the advantages of resource saving, low cost and the like. The content of the total tribulus terrestris saponins in the extract of the stems and the leaves of the tribulus terrestris is 90% or above.
Description
Technical field
The present invention relates to the extracting method of effective ingredient in a kind of plant stem-leaf, particularly relate to a kind of method extracting high-purity Fructus Tribuli Saponin in Fructus Tribuli stem and leaf.Belong to technical field of natural product extraction.
Background technology
Fructus Tribuli is the dry herb of zygophyllaceae plant Fructus Tribuli TribulusterrestrisL..Its fruit uses existing long medication history as Chinese medicine, has suppressing the hyperactive liver resolving depression, promoting blood circulation by removing wind, item, antipruritic effect, and for having a headache, dizzy, distending pain in the chest and hypochondrium, breast close the diseases such as acute mastitis, cataract, rubella pruritus.Recent studies proves; Fructus Tribuli also has defying age and strong effect, hypersexuality, the effect of raising sexuality, significantly can suppress the effect of people's mammary gland myeloid cell, hypoglycemic activity and the effect such as adjusting blood lipid, protection retinal neuronal cell except above effect, is a kind of Chinese crude drug of tunnel.It is the dry deburring fruit of Fructus Tribuli that existing market uses more, its when results stem and leaf as refuse process.Prove through modern medicine Technological research, the Fructus Tribuli Total saponin component content in its stem and leaf is all higher than content in fruit.Fructus Tribuli Total saponin common at present extracts and all adopts fruit to extract, and extraction cost is high, and treatment process is numerous and diverse.
Summary of the invention
The present invention is to provide a kind of method extracting Fructus Tribuli Total saponin from Fructus Tribuli stem and leaf, and it is simple that the method has extraction process, and the Fructus Tribuli total saponin content of extraction is high, extracts the advantages such as thorough.
The method problem that the present invention will provide solves by the following technical programs:
From Fructus Tribuli stem and leaf, extract a method for Fructus Tribuli Total saponin, comprise the following steps:
(1) Fructus Tribuli stem and leaf raw material is sieved, pulverize for subsequent use after the impurity such as removing dirt;
(2) first extracted with clean water, rear solvent method by the Fructus Tribuli stem and leaf after pulverizing, the extracting solution concentrating under reduced pressure obtained, concentrate is for subsequent use;
(3) concentrate purification, drying had both been obtained highly purified Fructus Tribuli Total saponin.
In order to reach better extraction effect,
In preferred step (1), Fructus Tribuli stem and leaf is crossed 20 mesh sieves in advance, remove the impurity such as dirt in advance.10-20 order is crushed to again with hammer crusher.This preprocess method can reduce the content of inorganic matter in end product.
In preferred step (2), soak 1 hour during clean water with the clear water of 8 times amount, in immersion process, once, circulate every 15min ON cycle 5min at every turn, discharges rinse water after 1 hour, plays and thoroughly go deimpurity effect.Solvent extraction method preferably carries out in following condition: Extraction solvent is ethanol: water 70:30, Extracting temperature 65-70 DEG C, 2 hours extraction times, extracts 2 times.Concentrating under reduced pressure is adopted, vacuum 0.04MPa time concentrated when extracting solution concentrates.Being concentrated to proportion is 1.15-1.18 Baume.
In preferred step (3), adopt the mode of water precipitating by concentrate purification, add the water of 10-12 times amount in concentrate, stir 15min, rear placement 5-8 hour, removes supernatant after layering.Bottom sediment thing is concentrated, drying both obtained finished product.
The advantages such as it is with low cost that extraction process of the present invention has extract, and extraction process is simple, and the total saponin content of extraction is high, and inorganic contaminants is few, can extract the extract obtaining Fructus Tribuli Total saponin more than 90% from Fructus Tribuli stem and leaf.
Accompanying drawing illustrates, Fig. 1 is a kind of process chart extracting the method for Fructus Tribuli Total saponin from Fructus Tribuli stem and leaf.
Claims (7)
1. from Fructus Tribuli stem and leaf, extract a method for Fructus Tribuli Total saponin, comprise the following steps:
(1) Fructus Tribuli stem and leaf is removed the impurity such as dirt in advance, then be crushed to 10-20 order with hammer crusher;
(2) raw material after pulverizing adopts extract by solvents, the extract concentrating under reduced pressure obtained, and concentrate is for subsequent use;
(3) by concentrate purification, dry, to obtain final product.
2. in accordance with the method for claim 1, it is characterized in that: the raw material used in step (1) is Fructus Tribuli stem and leaf, after crossing 20 mesh sieves in advance, be crushed to 10-20 order again.
3. in accordance with the method for claim 1, it is characterized in that: according to g/ml meter, the solid-liquid ratio of Fructus Tribuli stem and leaf and water is 1:8.
4. in accordance with the method for claim 1, it is characterized in that: the solvent extractable matter method described in step (2) is carried out in following condition: Extraction solvent is ethanol/water=70/30, Extracting temperature 65-70 DEG C, 2 hours extraction times, extract 2 times.
5. in accordance with the method for claim 1, it is characterized in that: vacuum 0.04MPa concentrated in step (2), proportion is 1.15-1.18 Baume.
6. in accordance with the method for claim 1, it is characterized in that the water adding 10-12 times amount in step (3) in concentrate, stir 15min, rear placement 5-8 hour, removes supernatant after layering, and bottom sediment thing is concentrated, drying both obtained finished product.
7. according to the method described in claim 4, it is characterized in that: described ethanol is medical alcohol, water is purified water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410364796.9A CN105311067A (en) | 2014-07-29 | 2014-07-29 | Method for extracting total tribulus terrestris saponins from stems and leaves of tribulus terrestris |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410364796.9A CN105311067A (en) | 2014-07-29 | 2014-07-29 | Method for extracting total tribulus terrestris saponins from stems and leaves of tribulus terrestris |
Publications (1)
| Publication Number | Publication Date |
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| CN105311067A true CN105311067A (en) | 2016-02-10 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410364796.9A Pending CN105311067A (en) | 2014-07-29 | 2014-07-29 | Method for extracting total tribulus terrestris saponins from stems and leaves of tribulus terrestris |
Country Status (1)
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| CN (1) | CN105311067A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1039595A (en) * | 1988-05-22 | 1990-02-14 | 洮南市第一制药厂 | The production technique of puncture vine total saponin |
| CN1413588A (en) * | 2002-06-27 | 2003-04-30 | 吉林省中医中药研究院 | Drug composition containing Fructus Tribuli fruit total saponin and its preparation method |
| CN1733199A (en) * | 2005-08-26 | 2006-02-15 | 彭勃 | Liver-protecting detoxification particles for curing drug induced liver injury and its production method |
| CN101234133A (en) * | 2007-11-09 | 2008-08-06 | 西安天健医药科学研究所 | Technique for using tribulus whole grass to extract total saponins of tribulus |
| CN101259153A (en) * | 2008-04-25 | 2008-09-10 | 江西远健药业有限公司 | Tribulus total steroid saponin and preparation thereof |
| CN102178706A (en) * | 2011-01-24 | 2011-09-14 | 南京泽朗医药科技有限公司 | Method for extracting tribuloside |
-
2014
- 2014-07-29 CN CN201410364796.9A patent/CN105311067A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1039595A (en) * | 1988-05-22 | 1990-02-14 | 洮南市第一制药厂 | The production technique of puncture vine total saponin |
| CN1413588A (en) * | 2002-06-27 | 2003-04-30 | 吉林省中医中药研究院 | Drug composition containing Fructus Tribuli fruit total saponin and its preparation method |
| CN1733199A (en) * | 2005-08-26 | 2006-02-15 | 彭勃 | Liver-protecting detoxification particles for curing drug induced liver injury and its production method |
| CN101234133A (en) * | 2007-11-09 | 2008-08-06 | 西安天健医药科学研究所 | Technique for using tribulus whole grass to extract total saponins of tribulus |
| CN101259153A (en) * | 2008-04-25 | 2008-09-10 | 江西远健药业有限公司 | Tribulus total steroid saponin and preparation thereof |
| CN102178706A (en) * | 2011-01-24 | 2011-09-14 | 南京泽朗医药科技有限公司 | Method for extracting tribuloside |
Non-Patent Citations (1)
| Title |
|---|
| 张素军: "蒺藜果实、茎叶不同采收期总皂苷含量分析", 《中国实验方剂学杂志》 * |
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Application publication date: 20160210 |
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