CN101259153A - Tribulus total steroid saponin and preparation thereof - Google Patents
Tribulus total steroid saponin and preparation thereof Download PDFInfo
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- CN101259153A CN101259153A CNA2008101068190A CN200810106819A CN101259153A CN 101259153 A CN101259153 A CN 101259153A CN A2008101068190 A CNA2008101068190 A CN A2008101068190A CN 200810106819 A CN200810106819 A CN 200810106819A CN 101259153 A CN101259153 A CN 101259153A
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Abstract
The present invention discloses a caltrop total steroid saponin which comprises caltrop total spirostanol saponin and caltrop total furostanol saponin and is characterized in that the content of the caltrop total furostanol saponin is more than 50 percent (W/W), which can meet the requirement of developing more than five kinds of new traditional Chinese medicines. The present invention also discloses a preparation method for the caltrop total steroid saponin, which has the following steps of (1) extracting ethanol; (2) filtering and concentrating; (3) absorbing macroporous resin; (4) washing pillar; (5) concentrating; (6) drying; (7) crushing, and the caltrop total steroid saponin can be obtained. The macroporous resin absorption means absorbing and concentrating the macroporous resin having the aperture of 90A degrees to 100A degrees and the specific surface area of 500m<2>/g to 550 m<2>/g above the filtrate of concentrated solution to obtain a midbody. The preparation method is simple; corresponding production equipments can be obtained easily; the production technology is grasped easily, which is especially suitable for industrialized mass production. The present invention collects the eluate with the ethanol having corresponding concentration by one step alcohol extracting-water precipitating and then concentrating and absorbing the macroporous resin for condensing, drying and crushing to obtain the caltrop total furostanol saponin.
Description
Technical field
The present invention relates to saponin, especially relate to a kind of tribulus total steroid saponin and preparation method thereof.
Background technology
The Chinese crude drug Fructus Tribuli is the dry mature fruit of zygophyllaceae plant Fructus Tribuli (Tribulus terrestris L.).Contain chemical constituents such as saponins, alkaloid, flavonoid class, nature and flavor suffering, hardship, tepor, slightly poisonous, return Liver Channel; Have suppressing the hyperactive liver resolving depression, promoting blood circulation by removing wind, make eye bright, antipruritic effect.Be usually used in having a headache dizzy, distending pain in the chest and hypochondrium, breast closes the treatment of diseases such as acute mastitis, conjunctival congestion cataracta.
Fructus Tribuli total saponins is a total steroid saponin in the Fructus Tribuli, comprises total spirostanol saponin and total furostanol two big classes, and the content of furostanol saponin in the tribulus total steroid saponin that different preparation methoies obtains is different." the containing pharmaceutical composition of Fructus Tribuli fruit total saponin and preparation method thereof " of Chinese patent CN1314402C bulletin, its pharmaceutical composition contains effective ingredient puncturevine furostanol saponins in the Fructus Tribuli fruit total saponin and the content of Fructus Tribuli spirostanol saponin is more than 50% (w/w).CN1830459A disclosed " tribulus terrestris extraction and its production and use " and for example, its tribulus terrestris extraction comprises content at total spirostanol saponin of the Fructus Tribuli more than 50% and the total furostanol of Fructus Tribuli, and wherein the total spirostanol saponin content of this Fructus Tribuli is 15%～40%; The total content of furostanol saponin of Fructus Tribuli is 15%～40%; Above-mentioned content is all in the extract gross weight.Be that the content that raw material is made the furostanol of the intermediate in the process of Xinnao Shutong preparation has only 25% with the aerial parts of Fructus Tribuli for another example.All can not reach the requirement of the above new Chinese medicine of five classes of research and development national regulation.Preparation method as for existing Fructus Tribuli total saponins also remains some shortcomings.Such as Chinese patent CN1039595A disclosed " Fructus Tribuli is converted the production technology of Saponin ", with sandbur is raw material, uses water as solvent, leaches through decocting in water, evaporation and concentration, separate with ethanol, again through resin absorption, separating water-soluble impurity, eluting then, reclaim ethanol, the impurity that contains in the spray drying gained Fructus Tribuli total saponins is on the high side, and content is on the low side.And for example publication number is CN1631420A disclosed " a kind of refining total saponins of tribulus for drug administration by injection, its preparation method and an injection ", though the content of Fructus Tribuli total saponins is very high, and preparation process complexity, trouble, and incompatibility industrialized great production.
Summary of the invention
A technical problem to be solved by this invention is the defective that remedies above-mentioned prior art, proposes a kind of intermediate-tribulus total steroid saponin that can be used as the above new Chinese medicine of five classes of research and development national regulation.
Another technical problem to be solved by this invention is the defective that remedies above-mentioned prior art, proposes a kind of preparation method of above-mentioned tribulus total steroid saponin, and this method is the extraction separation total saponins from the Chinese medicine Fructus Tribuli, and is simple, is particularly suitable for industrialized great production.
The technical problem of tribulus total steroid saponin of the present invention is solved by the following technical programs.
This tribulus total steroid saponin comprises total spirostanol saponin of Fructus Tribuli and the total furostanol of Fructus Tribuli.
The characteristics of this tribulus total steroid saponin are:
The total content of furostanol saponin of described Fructus Tribuli is more than 50% (W/W).
The technical problem of tribulus total steroid saponin preparation method of the present invention is solved by the following technical programs.
The characteristics of this tribulus total steroid saponin method are:
Following steps are arranged successively:
(1) ethanol extraction
The Fructus Tribuli raw medicinal herbs was pulverized No. two sieve into coarse powder, with 60%～80% (V/V) alcohol reflux 2 times～4 times, the ethanol consumption is 4～6 times of Fructus Tribuli crude drug amount, and extraction time is 1 hour～3 hours, and each time extracting solution is merged;
(2) filtering and concentrating
With extracting liquid filtering, be concentrated into and do not have the alcohol flavor, and with extracting solution be concentrated into every ml medicinal liquid be equivalent to 1 the gram～3 the gram the Fructus Tribuli raw medicinal herbs, 0 ℃～1 ℃ following cold preservation 10 hours～12 hours, be 4000～6000 rev/mins of following centrifugal filtrations 9 minutes～11 minutes with concentrated solution in speed again;
(3) macroporous resin adsorption
Concentrated solution filtrate after the centrifugal filtration is gone up the macroporous resin column adsorption and enrichment, obtain intermediate, the aperture of used macroporous resin column is that 90A °～100A °, specific surface area are 500m
2/ g～550m
2/ g;
(4) wash post
Be washed till the intermediate that adsorbs colourless with big water gaging earlier, and continue again to wash to sugar-free, it is negative that reducing sugar reaction shows, reuse 10%～20% (V/V) low-concentration ethanol is washed till the intermediate of absorption colourless, remove a part of fat-soluble impurity, use 60%～80% (V/V) ethanol elution then, collect eluent, reclaim ethanol to most;
(5) concentrate
Be concentrated into the thick paste that relative density is 1.15～1.25 (6 ℃) with washing the intermediate that post obtains;
(6) drying
Under 6 ℃～8 ℃, be dried to water content and be 4%～6% dried cream;
(7) pulverize
Pulverize 80 mesh sieves～100 mesh sieves with pulverizer and became fine powder, promptly got tribulus total steroid saponin.
The technical problem of tribulus total steroid saponin preparation method of the present invention is solved by the following technical programs.
Described step (1) is 70% (V/V) alcohol reflux of 6 times 3 times of Fructus Tribuli crude drug amount with consumption preferably, and extraction time is 2 hours.
Described step (2) preferably is concentrated into extracting solution the Fructus Tribuli raw medicinal herbs that every ml medicinal liquid is equivalent to 2 grams.
Described step (2) is 5000 rev/mins of following centrifugal filtrations 10 minutes with concentrated solution in speed preferably.
The used macroporous resin column of described step (3) is nonpolar macroporous resin column.
Described non-polar macroporous resin post comprises that model is the non-polar macroporous resin post of D101, AB-8, NKA, HPD100, HPD300, HPD100a, XAD-2, HP20.
The preferred model of described non-polar macroporous resin post is the non-polar macroporous resin post of D101, AB-8.
Described step (4) used 10%～20% (V/V) low-concentration ethanol preferred 15% (V/V) ethanol.
Described step (4) used 60%～80% (V/V) ethanol elution, preferred 75% (V/V) ethanol elution.
Described step (6) drying comprises constant pressure and dry, drying under reduced pressure, vacuum drying, spray drying, microwave drying.
Described drying preferably is decompressed to-0.06Mpa drying, vacuum drying.
The beneficial effect that the present invention is compared with the prior art is:
The total furostanol of Fructus Tribuli of the present invention, the content of its furostanol can reach the requirement of the above new Chinese medicine of five classes of research and development national regulation more than 50%.Preparation method of the present invention is simple, and corresponding production equipment is easy to get, and production technology is grasped easily, is particularly suitable for industrialized great production.The present invention collects the alcoholic acid eluent of respective concentration only by a step alcohol extracting-water precipitating, go up the macroporous resin column adsorption and enrichment then, again through concentrate, dry, pulverizing, promptly get the total furostanol of Fructus Tribuli.
The specific embodiment
Further specify the present invention below by the specific embodiment.
The total content of furostanol saponin of Fructus Tribuli in the tribulus total steroid saponin of the present invention, be to measure according to the method that content of furostanol saponin in the appended Fructus Tribuli crude saponin quality standard of Xinnao Shutong sheet of " national standard for traditional Chinese medicines compilation-internal medicine is felt concerned about fascicle " (State Food and Drug Administration's volume) record is measured under the item, concrete operations are as follows:
Get an amount of W1 of this product (being equivalent to furostanol 20mg approximately), the accurate title, decide, and puts in the tool plug conical flask, precision adds methanol 25ml, claims to decide weight, reflux, extract, 20 minutes, put cold, claim again to decide weight, supply the weight that subtracts mistake, shake up with methanol, filter, discard filtrate just, the accurate subsequent filtrate 2ml that draws puts in the tool plug test tube, the accurate methanol 3ml that adds, improvement paradime thylaminobenzaldehyde solution 5ml, close plug shakes up, put in 5 ℃ of water-baths and heated 2 hours, cooling rapidly replaces need testing solution with methanol, makes blank solution with method.According to ultraviolet visible spectrophotometry (" Chinese pharmacopoeia version in 2005, first appendix VA), measure trap A1 at the wavelength place of 515mm.Other gets cobaltous chloride (CoCl
26H
2O) 0.55g (W2), the accurate title, decide, put in the 25ml measuring bottle, adding water makes dissolving and is diluted to scale, shake up, with water is blank, measures trap A2 at 515mm wavelength place, is calculated as follows the percentage composition of furostanol: percentage composition (%)=(A1 * 5 * W2 * 0.0093)/(A2 * W1) * 100%.0.0093 is meant every 1g cobaltous chloride (CoCl in the formula
26H
2O) amount to furostanol 0.0093g.
The preparation method of one first kind of tribulus total steroid saponin of the specific embodiment
Get Fructus Tribuli medical material 1kg, each 60% alcohol reflux 2 times (1 hour/time) with 4 times of amounts, merge extractive liquid,, filter, filtrate is concentrated into does not have the alcohol flavor, and transfers concentrated solution volume to every ml medicinal liquid to be equivalent to the crude drug of 1 gram, 0 ℃ of following cold preservation 10 hours, centrifugal filtration, getting the last model of filtrate is the D101 macroporous resin column; Behind the upper prop, make it abundant absorption, when washing with water to colourless and the apparent feminine gender of reducing sugar reaction earlier, reuse 20% ethanol is washed till colourless, uses 80% ethanol elution then, collect eluent, reclaim ethanol to most, concentrating under reduced pressure, drying, pulverizing promptly get tribulus total steroid saponin, measure through preceding method, containing furostanol is 65.2%.
The preparation method of 2 second kinds of tribulus total steroid saponins of the specific embodiment
Get Fructus Tribuli medical material 1kg, each 70% alcohol reflux 2 times (2 hours/time) with 5 times of amounts, merge extractive liquid,, filter, filtrate is concentrated into does not have the alcohol flavor, and transfers concentrated solution volume to every ml medicinal liquid to be equivalent to the crude drugs of 2 grams, 5 ℃ of following cold preservation 12 hours, centrifugal filtration, getting the last model of filtrate is the AB-8 macroporous resin column; Behind the upper prop, make it abundant absorption, when washing with water to colourless and the apparent feminine gender of reducing sugar reaction earlier, reuse 10% ethanol is washed till colourless, uses 70% ethanol elution then, collect eluent, reclaim ethanol to the greatest extent, concentrate, constant pressure and dry, pulverizing, promptly get tribulus total steroid saponin, measure through preceding method, containing furostanol is 59.3%.
The preparation method of the specific embodiment three the third tribulus total steroid saponins
Get Fructus Tribuli medical material 1kg, each 80% alcohol reflux 2 times (3 hours/time) with 6 times of amounts, merge extractive liquid,, filter, filtrate is concentrated into does not have the alcohol flavor, and transfers concentrated solution volume to every ml medicinal liquid to be equivalent to the crude drugs of 3 grams, 1 ℃ of following cold preservation 12 hours, centrifugal filtration, getting the last model of filtrate is the NKA-9 macroporous resin column; Behind the upper prop, make it abundant absorption, when washing with water to colourless and the apparent feminine gender of reducing sugar reaction earlier, reuse 15% ethanol is washed till colourless, uses 75% ethanol elution then, collect eluent, reclaim ethanol to most, concentrated, spray drying promptly get tribulus total steroid saponin, measure through preceding method, containing furostanol is 63.5%.
The preparation method of the 4th kind of tribulus total steroid saponin of the specific embodiment 4
Get Fructus Tribuli medical material 1kg, each 60% alcohol reflux 3 times (3 hours/time) with 5 times of amounts, merge extractive liquid,, filter, filtrate is concentrated into does not have the alcohol flavor, and transfers concentrated solution volume to every ml medicinal liquid to be equivalent to the crude drugs of 2 grams, 5 ℃ of following cold preservation 10 hours, centrifugal filtration, getting the last model of filtrate is the HPD100 macroporous resin column; Behind the upper prop, make it abundant absorption, when washing with water to colourless and the apparent feminine gender of reducing sugar reaction earlier, reuse 10% ethanol is washed till colourless, uses 60% ethanol elution then, collect eluent, reclaim ethanol to the greatest extent, concentrate, constant pressure and dry, pulverizing, promptly get tribulus total steroid saponin, measure through preceding method, containing furostanol is 55.9%.
The preparation method of the 5th kind of tribulus total steroid saponin of the specific embodiment 5
Get Fructus Tribuli medical material 1kg, each 70% alcohol reflux 3 times (1 hour/time) with 6 times of amounts, merge extractive liquid,, filter, filtrate is concentrated into does not have the alcohol flavor, and transfers concentrated solution volume to every ml medicinal liquid to be equivalent to the crude drug of 1 gram, 4 ℃ of following cold preservation 10 hours, centrifugal filtration, getting the last model of filtrate is the HP20 macroporous resin column; Behind the upper prop, make it abundant absorption, when washing with water to colourless and the apparent feminine gender of reducing sugar reaction earlier, reuse 20% ethanol is washed till colourless, uses 75% ethanol elution then, collect eluent, reclaim ethanol to the greatest extent, concentrate, microwave drying, pulverizing, promptly get tribulus total steroid saponin, measure through preceding method, containing furostanol is 62.8%.
The preparation method of the 6th kind of tribulus total steroid saponin of the specific embodiment 6
Get Fructus Tribuli medical material 1kg, each 70% alcohol reflux 3 times (2 hours/time) with 4 times of amounts, merge extractive liquid,, filter, filtrate is concentrated into does not have the alcohol flavor, and transfers concentrated solution volume to every ml medicinal liquid to be equivalent to the crude drugs of 2.5 grams, 6 ℃ of following cold preservation 11 hours, centrifugal filtration, getting the last model of filtrate is the SH825 macroporous resin column; Behind the upper prop, make it abundant absorption, when washing with water to colourless and the apparent feminine gender of reducing sugar reaction earlier, reuse 10% ethanol is washed till colourless, use 65% ethanol elution then, collect eluent, reclaim ethanol, continue to be concentrated into thick paste to the greatest extent, microwave drying, promptly get tribulus total steroid saponin, measure through preceding method, containing furostanol is 61.4%.
The preparation method of the 7th kind of tribulus total steroid saponin of the specific embodiment 7
Get Fructus Tribuli medical material 1kg, each 60% alcohol reflux 4 times (2 hours/time) with 6 times of amounts, merge extractive liquid,, filter, filtrate is concentrated into does not have the alcohol flavor, and transfers concentrated solution volume to every ml medicinal liquid to be equivalent to the crude drugs of 1.5 grams, 8 ℃ of following cold preservation 12 hours, centrifugal filtration, getting the last model of filtrate is the D101 macroporous resin column; Behind the upper prop, make it abundant absorption, when washing with water to colourless and the apparent feminine gender of reducing sugar reaction earlier, reuse 20% ethanol is washed till colourless, uses 80% ethanol elution then, collect eluent, reclaim ethanol to most, concentrated, vacuum drying promptly get tribulus total steroid saponin, measure through preceding method, containing furostanol is 65.7%.
The preparation method of the 8th kind of tribulus total steroid saponin of the specific embodiment 8
Get Fructus Tribuli medical material 1kg, each 70% alcohol reflux 4 times (3 hours/time) with 4 times of amounts, merge extractive liquid,, filter, filtrate is concentrated into does not have the alcohol flavor, and transfers concentrated solution volume to every ml medicinal liquid to be equivalent to the crude drugs of 2 grams, 5 ℃ of following cold preservation 11 hours, centrifugal filtration, getting the last model of filtrate is the HPD100 macroporous resin column; Behind the upper prop, make it abundant absorption, when washing with water to colourless and the apparent feminine gender of reducing sugar reaction earlier, reuse 20% ethanol is washed till colourless, uses 80% ethanol elution then, collect eluent, reclaim ethanol to the greatest extent, concentrate, be decompressed to-the 0.06Mpa drying, pulverizing, promptly get tribulus total steroid saponin, measure through preceding method, containing furostanol is 64.4%.
The preparation method of the 9th kind of tribulus total steroid saponin of the specific embodiment 9
Get Fructus Tribuli medical material 1kg, each 80% alcohol reflux 4 times (1 hour/time) with 5 times of amounts, merge extractive liquid,, filter, filtrate is concentrated into does not have the alcohol flavor, and transfers concentrated solution volume to every ml medicinal liquid to be equivalent to the crude drugs of 2 grams, 1 ℃ of following cold preservation 12 hours, centrifugal filtration, getting the last model of filtrate is the AB-8 macroporous resin column; Behind the upper prop, make it abundant absorption, when washing with water to colourless and the apparent feminine gender of reducing sugar reaction earlier, reuse 15% ethanol is washed till colourless, uses 75% ethanol elution then, collect eluent, reclaim ethanol to the greatest extent, concentrate, constant pressure and dry, pulverizing, promptly get tribulus total steroid saponin, measure through preceding method, containing furostanol is 61.8%.
Above content be in conjunction with concrete preferred implementation to further describing that the present invention did, can not assert that concrete enforcement of the present invention is confined to these explanations.For the general technical staff of the technical field of the invention, without departing from the inventive concept of the premise, its framework form can be flexible and changeable, can the subseries product.Just make some simple deduction or replace, all should be considered as belonging to the scope of patent protection that the present invention is determined by claims of being submitted to.
Claims (10)
1. a tribulus total steroid saponin comprises total spirostanol saponin of Fructus Tribuli and the total furostanol of Fructus Tribuli, it is characterized in that:
The total content of furostanol saponin of described Fructus Tribuli is more than 50% (W/W).
2. the preparation method of a tribulus total steroid saponin is characterized in that:
Following steps are arranged successively:
(1) ethanol extraction
The Fructus Tribuli raw medicinal herbs was pulverized No. two sieve into coarse powder, with 60%～80% (V/V) alcohol reflux 2 times～4 times, the ethanol consumption is 4～6 times of Fructus Tribuli crude drug amount, and extraction time is 1 hour～3 hours, and each time extracting solution is merged;
(2) filtering and concentrating
With extracting liquid filtering, be concentrated into and do not have the alcohol flavor, and with extracting solution be concentrated into every ml medicinal liquid be equivalent to 1 the gram～3 the gram the Fructus Tribuli raw medicinal herbs, 0 ℃～1 ℃ following cold preservation 10 hours～12 hours, be 4000～6000 rev/mins of following centrifugal filtrations 9 minutes～11 minutes with concentrated solution in speed again;
(3) macroporous resin adsorption
Concentrated solution filtrate after the centrifugal filtration is gone up the macroporous resin column adsorption and enrichment, obtain intermediate, the aperture of used macroporous resin column is that 90A °～100A °, specific surface area are 500m
2/ g～550m
2/ g;
(4) wash post
Be washed till the intermediate that adsorbs colourless with big water gaging earlier, and continue again to wash to sugar-free, it is negative that reducing sugar reaction shows, reuse 10%～20% (V/V) low-concentration ethanol is washed till the intermediate of absorption colourless, remove a part of fat-soluble impurity, use 60%～80% (V/V) ethanol elution then, collect eluent, reclaim ethanol to most;
(5) concentrate
Be concentrated into the thick paste that relative density is 1.15～1.25 (6 ℃) with washing the intermediate that post obtains;
(6) drying
Under 6 ℃～8 ℃, be dried to water content and be 4%～6% dried cream;
(7) pulverize
Pulverize 80 mesh sieves～100 mesh sieves with pulverizer and became fine powder, promptly got tribulus total steroid saponin.
3. the preparation method of tribulus total steroid saponin according to claim 2 is characterized in that:
Described step (1) is to be 70% (V/V) alcohol reflux of 6 times 3 times of Fructus Tribuli crude drug amount with consumption, and extraction time is 2 hours.
4. according to the preparation method of claim 2 or 3 described tribulus total steroid saponins, it is characterized in that:
Described step (2) is that extracting solution is concentrated into the Fructus Tribuli raw medicinal herbs that every ml medicinal liquid is equivalent to 2 grams.
5. the preparation method of tribulus total steroid saponin according to claim 4 is characterized in that:
Described step (2) is to be 5000 rev/mins of following centrifugal filtrations 10 minutes with concentrated solution in speed.
6. the preparation method of tribulus total steroid saponin according to claim 5 is characterized in that:
The used macroporous resin column of described step (3) is nonpolar macroporous resin column.
7. the preparation method of tribulus total steroid saponin according to claim 6 is characterized in that:
Described non-polar macroporous resin post comprises that model is the non-polar macroporous resin post of D101, AB-8, NKA, HPD100, HPD300, HPD100a, XAD-2, HP20.
8. the preparation method of tribulus total steroid saponin according to claim 7 is characterized in that:
It is 10%～20% (V/V) with the concentration of low-concentration ethanol that described step (4) is washed post.
9. the preparation method of tribulus total steroid saponin according to claim 8 is characterized in that:
The concentration of alcohol that described step (4) is collected ethanol elution is 60%～80% (V/V).
10. the preparation method of tribulus total steroid saponin according to claim 8 is characterized in that:
Described step (6) drying comprises constant pressure and dry, drying under reduced pressure, vacuum drying, spray drying, microwave drying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008101068190A CN101259153A (en) | 2008-04-25 | 2008-04-25 | Tribulus total steroid saponin and preparation thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008101068190A CN101259153A (en) | 2008-04-25 | 2008-04-25 | Tribulus total steroid saponin and preparation thereof |
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| CN101259153A true CN101259153A (en) | 2008-09-10 |
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ID=39959983
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103040880A (en) * | 2012-12-13 | 2013-04-17 | 大兴安岭林格贝有机食品有限责任公司 | Separation and purification method of tribulus terrestris saponin |
| CN103734717A (en) * | 2013-12-19 | 2014-04-23 | 江西安正利康生命科技有限公司 | Method for preparing nutrient food capable of improving immunity and relieving fatigue |
| CN105311067A (en) * | 2014-07-29 | 2016-02-10 | 西安旭煌生物技术有限公司 | Method for extracting total tribulus terrestris saponins from stems and leaves of tribulus terrestris |
| CN107296818A (en) * | 2017-06-15 | 2017-10-27 | 吉林大学 | A kind of method that total furostanol saponin and total spirostanol saponin are prepared from puncture vine |
| CN113134020A (en) * | 2021-05-14 | 2021-07-20 | 内蒙古民族大学 | Preparation method and application of Mongolian medicine tribulus terrestris ethanol extract |
-
2008
- 2008-04-25 CN CNA2008101068190A patent/CN101259153A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103040880A (en) * | 2012-12-13 | 2013-04-17 | 大兴安岭林格贝有机食品有限责任公司 | Separation and purification method of tribulus terrestris saponin |
| CN103040880B (en) * | 2012-12-13 | 2017-10-10 | 大兴安岭林格贝寒带生物科技股份有限公司 | A kind of isolation and purification method of tribuloside |
| CN103734717A (en) * | 2013-12-19 | 2014-04-23 | 江西安正利康生命科技有限公司 | Method for preparing nutrient food capable of improving immunity and relieving fatigue |
| CN105311067A (en) * | 2014-07-29 | 2016-02-10 | 西安旭煌生物技术有限公司 | Method for extracting total tribulus terrestris saponins from stems and leaves of tribulus terrestris |
| CN107296818A (en) * | 2017-06-15 | 2017-10-27 | 吉林大学 | A kind of method that total furostanol saponin and total spirostanol saponin are prepared from puncture vine |
| CN113134020A (en) * | 2021-05-14 | 2021-07-20 | 内蒙古民族大学 | Preparation method and application of Mongolian medicine tribulus terrestris ethanol extract |
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