CN101791335A - Total alkaloid mixture sourcing from Chinese medicina plant lotus plumule and extraction and preparation method thereof - Google Patents
Total alkaloid mixture sourcing from Chinese medicina plant lotus plumule and extraction and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a lotus nut total alkaloid, comprising liensinine, isoliensinine and neferine, wherein the total content of the lotus nut total alkaloid in the three items is more than 50% (W/W), and according to the total weight of the extract, the content of the liensinine is not less than 5%-40%, the content of the isoliensinine is not less than 1%-20%, the content of the neferine is not less than 5%-30%, thus being capable of meeting the requirement of developing more than five categories of new traditional Chinese medicines. The invention also discloses a preparation method for lotus nut total alkaloid, comprising the following steps: acidic ethanol extraction, filtration and concentration, macroporous resin adsorption, column washing, concentration, drying and smashing, thus obtaining the lotus nut total alkaloid. The step of macroporous resin adsorption is to obtain an intermediate through enriching macroporous resin columns with the aperture of 90 angstrom-100 angstrom and the specific surface area of 500m<2>/g-550m<2>/g in the filter liquor of the concentrated liquor. The invention has simple preparation method, easily obtained corresponding production equipment and easily grasped production technology, thus being particularly suitable for industrialized production.
Description
Technical field
The present invention relates to liensinine, especially relate to a kind of Plumula Nelumbinis total alkali mixture and extraction preparation method thereof that comprises liensinine, isoliensinine, (-)-Neferine.
Background technology
The Chinese crude drug Plumula Nelumbinis is dry immature leaf and the radicle in the dry mature seed of Nymphaeceae section plant lotus (Nelumbo nucifera Gaertn.).Contain chemical constituents such as alkaloid (mainly comprising liensinine, (-)-Neferine, isoliensinine etc.), flavonoid, nature and flavor hardship, cold, GUIXIN, kidney channel; The traditional Chinese medical science thinks that Plumula Nelumbinis has heat clearing away, and controlling nocturnal emission with astringent drugs is calmed the nerves, heart tonifying, hemostasis, the effect of arresting seminal emission can be controlled the dysphoria that hyperpyrexia causes, diseases such as unconsciousness and emission. contain alkaloid in the Plumula Nelumbinis, can also hypotensive activity, also have simultaneously clear away heart-fire, calm the nerves, restoring normal coordination between the heart and kidney, unsmoothing the sperm and stopping bleeding.Be used for heat attacking the pericardium, coma toad language, disarmony between the heart and kidney, insomnia seminal emission, the treatment that heat in blood is spitted blood.
Contained Plumula Nelumbinis total alkali is mainly the total alkali mixture of liensinine, (-)-Neferine, isoliensinine etc. in the Plumula Nelumbinis medical material, comprise three major types such as liensinine, (-)-Neferine, isoliensinine, Plumula Nelumbinis total alkali in the Plumula Nelumbinis total alkali that different preparation methoies obtains is different, and the ratio of wherein total main three kinds of liensinines, (-)-Neferine, isoliensinine also can be different.Can not meet the specification requirement of country, all can not reach the requirement of the above new Chinese medicine of exploitation five classes for novelty Chinese medicine research five class Chinese medicines.Preparation method as for existing Plumula Nelumbinis total alkali also remains some shortcomings.Though it is very high to obtain the content of liensinine, belong to the alkaloid height of certain single component wherein, and preparation process complexity, trouble, and the incompatibility industrialized great production.
Summary of the invention
A technical problem to be solved by this invention is the defective that remedies above-mentioned prior art, proposes a kind of new intermediate---Plumula Nelumbinis total alkali that can be used as the above new Chinese medicine of five classes of researching and developing national regulation.
Another technical problem to be solved by this invention is the defective that remedies above-mentioned prior art, propose a kind of from the Chinese medicine Plumula Nelumbinis extraction separation Plumula Nelumbinis total alkali, simple, be particularly suitable for the preparation method of the above-mentioned Plumula Nelumbinis total alkali of industrialized great production.
The total alkali mixture of source provided by the invention Chinese crude drug Plumula Nelumbinis comprises liensinine, isoliensinine, and (-)-Neferine, the content of its Plumula Nelumbinis total alkali is more than 50%W/W.Wherein in extract gross weight liensinine content be not less than 5%-40%, isoliensinine content is not less than 1%-20% (-)-Neferine content and is not less than 5%-30%.
The extraction preparation method of the total alkali mixture of source of the present invention Chinese crude drug Plumula Nelumbinis may further comprise the steps successively: (1) acidic ethanol extraction: the Plumula Nelumbinis raw medicinal herbs was pulverized No. two of 2000 editions pharmacopeia regulation sieve into coarse powder, with the acidic ethanol reflux, extract, of 60%～80%V/V, pH=1-3 2～4 times, the acidic ethanol consumption is 4～6 times of Plumula Nelumbinis crude drug amount, extraction time is 1～3 hour, and each time extracting solution is merged;
(2) filtering and concentrating: with extracting liquid filtering, be concentrated into and do not have the alcohol flavor, and with extracting solution be concentrated into every ml medicinal liquid be equivalent to 1 the gram～3 the gram the Plumula Nelumbinis raw medicinal herbs, 0 ℃～10 ℃ following cold preservations 10 hours～12 hours, be 4000～6000 rev/mins of following centrifugal filtrations 9 minutes～11 minutes with concentrated solution in speed again;
(3) macroporous resin adsorption: the concentrated solution filtrate after the centrifugal filtration is gone up the macroporous resin column adsorption and enrichment, obtain intermediate;
(4) wash post: be washed till the intermediate that adsorbs colourless with big water gaging earlier, and continue again to wash to sugar-free, it is negative that reducing sugar reaction shows, reuse 10%～20%V/V low-concentration ethanol is washed till the intermediate of absorption colourless, remove a part of fat-soluble impurity, use the acidic ethanol eluting of 60%～80%V/V, pH=1-3 then, collect eluent, reclaim ethanol to most;
(5) concentrate: will be neutralized to pH=8-9 with the eluent that 60%～80%V/V acidic ethanol elutes and be concentrated into the thick paste that relative density is 1.15～1.25 (60 ℃);
(6) drying: under 60 ℃～80 ℃, be dried to water content and be 4%～6% dried cream;
(7) pulverize, sieve, promptly.
The aperture of the macroporous adsorptive resins that the present invention is used is that 90A °～100A °, specific surface area are 500m
2/ g～550m
2/ g
Step of the present invention (1) is to be 70% (V/V) acidic ethanol reflux, extract, of 6 times 3 times of Plumula Nelumbinis crude drug amount with consumption, and extraction time is 2 hours.
Step of the present invention (2) is that extracting solution is concentrated into the Plumula Nelumbinis raw medicinal herbs that every ml medicinal liquid is equivalent to 2 grams.
Described step of the present invention (2) is to be 5000 rev/mins of following centrifugal filtrations 10 minutes with concentrated solution in speed.
The used macroporous adsorptive resins of described step of the present invention (3) is a nonpolarity macroporous adsorptive resins chromatographic column.Be D101 or AB-8 or NKA or HPD100, HPD300 or HPD100a or XAD-2 or HP20.
Described step of the present invention (6) drying comprises constant pressure and dry, drying under reduced pressure, vacuum drying, spray drying, microwave drying, vacuum microwave drying.
The beneficial effect that the present invention is compared with the prior art is: Plumula Nelumbinis total alkali of the present invention, comprise liensinine, (-)-Neferine, isoliensinine etc., and its total content of three can reach the specification requirement of the above oral Chinese medicine new drug of exploitation five classes more than 50%.
Preparation method of the present invention is simple, and corresponding production equipment is easy to get, and production technology is grasped easily, is particularly suitable for industrialized great production.The present invention collects the alcoholic acid eluent of respective concentration only by a step alcohol extracting-water precipitating, go up the macroporous adsorptive resins enrichment then, again through concentrate, dry, pulverizing, promptly get the Plumula Nelumbinis total alkali.
The specific embodiment
Further specify the present invention below by the specific embodiment.
Content in the Plumula Nelumbinis total alkali of extraction separation gained of the present invention, be according to " under a Plumula Nelumbinis quality of medicinal material of the 2010 editions Pharmacopoeias of People's Republic of China normal term in the Plumula Nelumbinis quality of medicinal material standard of P256 record the method under the assay item measure, concrete operations are as follows:
[assay] is according to high performance liquid chromatography (appendix VI D)
Chromatographic condition and system suitability test are filler with the octadecylsilane chemically bonded silica; With acetonitrile-0.015mol/L sodium dodecyl sulfate solution (get dodecyl sodium sulfate 0.41g, add water 100ml and make dissolving)-glacial acetic acid (56: 43: 1) is mobile phase; The detection wavelength is 282nm.Number of theoretical plate calculates by the liensinine peak should be not less than 5000.
It is an amount of that [preparation of reference substance solution] gets liensinine perchlorate reference substance, and accurate the title decides, and adds methanol and makes the solution that every 1ml contains 25ug, promptly gets (liensinine weight=liensinine perchlorate weight/1.3587).
[preparation of need testing solution] gets the about 0.5g of this product powder (crossing sieve No. four), and accurate the title decides, the accurate 2% methanol hydrochloride solution 25ml that adds, claim to decide weight, reflux 30 minutes is put coldly, claims to decide weight again, supply the weight that subtracts mistake with 2% methanol hydrochloride solution, shake up, precision is measured subsequent filtrate 5ml and is put in the 10mL measuring bottle, adds mobile phase and is diluted to scale, shake up, promptly.
[algoscopy] accurate respectively reference substance solution and each 10ul of need testing solution of drawing injects chromatograph of liquid, measures, promptly.
Isoliensinine (Isoliensinine)
CAS:6817-41-0 source plant: Plumula Nelumbinis Lotus Rhizome Node.
Molecular formula: C37H42N2O6, structural formula:
And (-)-Neferine (Neferine), carry out assay with reference to method once.
[assay]: with Hypsil C18 (4.6mm * 250mm, 5 μ m) is immobile phase, and methanol-phosphate buffer (73: 27, pH9.0) be mobile phase, detecting wavelength is 286nm and 230nm, flow velocity 1mLmin^-1.The result: 3 kinds of alkaloids separate well, at 20～700 μ gmL^-1.Linear relationship is good.Its 24 hours, 48 hours RSD is at 0.24%～4.83%, and two kinds of alkaloidal average recoveries are respectively 98.4%, 98.1%.Utilize this method to measure isoliensinine and two kinds of alkaloids of (-)-Neferine in the Plumula Nelumbinis total alkali that the different batches distinct methods extracts, the result shows the content that this law is simple, quick, be suitable for measuring isoliensinine in the Plumula Nelumbinis, (-)-Neferine.
The pre-treatment of sample: get an amount of W1 of this product (being equivalent to Plumula Nelumbinis total alkali 2mg approximately), the accurate title, decide, and puts in the tool plug conical flask, precision adds methanol 25ml, claim to decide weight, reflux, extract, 20 minutes is put cold, claim to decide weight again, supply the weight that subtracts mistake with methanol, shake up, filter, discard filtrate just, the accurate subsequent filtrate 2ml that draws puts in the tool plug test tube, the accurate methanol 3ml that adds, close plug, shake up, put in 58 ± 1 ℃ of water-baths and heated 2 hours, rapidly cooling, replace need testing solution with methanol, make blank solution with method.Measure according to said method.
The present invention is described in detail below in conjunction with embodiment.
The preparation method of 1 first kind of Plumula Nelumbinis total alkali of embodiment
Get exsiccant Plumula Nelumbinis medical material 1000g, pulverized 10 mesh sieves, each acidity 60% ethanol (pH=2) reflux, extract, 2 times (1 hour/time) with 4 times of amounts, merge extractive liquid, filters, and filtrate is concentrated into does not have the alcohol flavor, and transfer concentrated solution volume to every ml medicinal liquid to be equivalent to the crude drug of 1 gram, cold preservation below 0 ℃ 10 hours, centrifugal filtration is got filtrate and is gone up macroporous adsorptive resins (D101); Behind the upper prop, make it abundant absorption, when washing with water to colourless and the apparent feminine gender of reducing sugar reaction earlier, reuse 20% ethanol is washed till colourless, uses 80% acidic ethanol eluting then, collect eluent, reclaim ethanol to most, concentrating under reduced pressure, drying, pulverizing promptly get the Plumula Nelumbinis total alkali, measure through preceding method, containing the Plumula Nelumbinis total alkali is 65.2%.
The preparation method of 2 second kinds of Plumula Nelumbinis total alkalis of embodiment
Get Plumula Nelumbinis medical material 1kg, each 70% acidic ethanol (ph=2) reflux, extract, 2 times (2 hours/time) with 5 times of amounts, merge extractive liquid,, filter, filtrate is concentrated into does not have the alcohol flavor, and transfers concentrated solution volume to every ml medicinal liquid to be equivalent to the crude drugs of 2 grams, 5 ℃ of following cold preservation 12 hours, centrifugal filtration is got filtrate and is gone up macroporous adsorptive resins (AB-8); Behind the upper prop, make it abundant absorption, when washing with water to colourless and the apparent feminine gender of reducing sugar reaction earlier, reuse 10% ethanol is washed till colourless, uses 70% acidic ethanol eluting then, collect eluent, reclaim ethanol to the greatest extent, concentrate, constant pressure and dry, pulverizing, promptly get the Plumula Nelumbinis total alkali, measure through preceding method, containing the Plumula Nelumbinis total alkali is 59.3%.
Embodiment 3: the preparation method of the third Plumula Nelumbinis total alkali
Get Plumula Nelumbinis medical material 1kg, each 80% acidic ethanol reflux, extract, 2 times (3 hours/time) with 6 times of amounts, merge extractive liquid,, filter, filtrate is concentrated into does not have the alcohol flavor, and transfers concentrated solution volume to every ml medicinal liquid to be equivalent to the crude drugs of 3 grams, 10 ℃ of following cold preservation 12 hours, centrifugal filtration is got filtrate and is gone up macroporous adsorptive resins (NKA-9); Behind the upper prop, make it abundant absorption, when washing with water to colourless and the apparent feminine gender of reducing sugar reaction earlier, reuse 15% ethanol is washed till colourless, uses 75% acidic ethanol eluting then, collect eluent, reclaim ethanol to most, concentrated, spray drying promptly get tribulus total steroid saponin, measure through preceding method, containing the Plumula Nelumbinis total alkali is 63.5%.
The preparation method of the 4th kind of Plumula Nelumbinis total alkali of embodiment 4
Get Plumula Nelumbinis medical material 1kg, each 60% acidic ethanol reflux, extract, 3 times (3 hours/time) with 5 times of amounts, merge extractive liquid,, filter, filtrate is concentrated into does not have the alcohol flavor, and transfers concentrated solution volume to every ml medicinal liquid to be equivalent to the crude drugs of 2 grams, 5 ℃ of following cold preservation 10 hours, centrifugal filtration is got filtrate and is gone up macroporous adsorptive resins (HPD100); Behind the upper prop, make it abundant absorption, when washing with water to colourless and the apparent feminine gender of reducing sugar reaction earlier, reuse 10% ethanol is washed till colourless, uses 60% acidic ethanol eluting then, collect eluent, reclaim ethanol to the greatest extent, concentrate, constant pressure and dry, pulverizing, promptly get tribulus total steroid saponin, measure through preceding method, containing the Plumula Nelumbinis total alkali is 55.9%.
The preparation method of the 5th kind of Plumula Nelumbinis total alkali of embodiment 5
Get Plumula Nelumbinis medical material 1kg, each 70% acidic ethanol reflux, extract, 3 times (1 hour/time) with 6 times of amounts, merge extractive liquid,, filter, filtrate is concentrated into does not have the alcohol flavor, and transfers concentrated solution volume to every ml medicinal liquid to be equivalent to the crude drug of 1 gram, 4 ℃ of following cold preservation 10 hours, centrifugal filtration is got filtrate and is gone up macroporous adsorptive resins (HP20); Behind the upper prop, make it abundant absorption, when washing with water to colourless and the apparent feminine gender of reducing sugar reaction earlier, reuse 20% ethanol is washed till colourless, uses 75% ethanol elution then, collect eluent, reclaim ethanol to the greatest extent, concentrate, microwave drying, pulverizing, promptly get the Plumula Nelumbinis total alkali, measure through preceding method, containing the Plumula Nelumbinis total alkali is 62.8%.
The preparation method of the 6th kind of Plumula Nelumbinis total alkali of embodiment 6
Get Plumula Nelumbinis medical material 1kg, each 70% acidic ethanol reflux, extract, 3 times (2 hours/time) with 4 times of amounts, merge extractive liquid,, filter, filtrate is concentrated into does not have the alcohol flavor, and transfers concentrated solution volume to every ml medicinal liquid to be equivalent to the crude drugs of 2.5 grams, 6 ℃ of following cold preservation 11 hours, centrifugal filtration is got filtrate and is gone up macroporous adsorptive resins (SH825); Behind the upper prop, make it abundant absorption, when washing with water to colourless and the apparent feminine gender of reducing sugar reaction earlier, reuse 10% ethanol is washed till colourless, use 65% acidic ethanol eluting then, collect eluent, reclaim ethanol, continue to be concentrated into thick paste to the greatest extent, microwave drying, promptly get tribulus total steroid saponin, measure through preceding method, containing the Plumula Nelumbinis total alkali is 61.4%.
The preparation method of the 7th kind of Plumula Nelumbinis total alkali of embodiment 7
Get Plumula Nelumbinis medical material 1kg, each 60% acidic ethanol reflux, extract, 4 times (2 hours/time) with 6 times of amounts, merge extractive liquid,, filter, filtrate is concentrated into does not have the alcohol flavor, and transfers concentrated solution volume to every ml medicinal liquid to be equivalent to the crude drugs of 1.5 grams, 8 ℃ of following cold preservation 12 hours, centrifugal filtration is got filtrate and is gone up macroporous adsorptive resins (D101); Behind the upper prop, make it abundant absorption, when washing with water to colourless and the apparent feminine gender of reducing sugar reaction earlier, reuse 20% ethanol is washed till colourless, uses 80% ethanol elution then, collect eluent, reclaim ethanol to most, concentrated, vacuum drying promptly get the Plumula Nelumbinis total alkali, measure through preceding method, containing the Plumula Nelumbinis total alkali is 65.7%.
The preparation method of the 8th kind of Plumula Nelumbinis total alkali of embodiment 8
Get Plumula Nelumbinis medical material 1kg, each 70% acidic ethanol reflux, extract, 4 times (3 hours/time) with 4 times of amounts, merge extractive liquid,, filter, filtrate is concentrated into does not have the alcohol flavor, and transfers concentrated solution volume to every ml medicinal liquid to be equivalent to the crude drugs of 2 grams, 5 ℃ of following cold preservation 11 hours, centrifugal filtration is got filtrate and is gone up macroporous adsorptive resins (HPD100); Behind the upper prop, make it abundant absorption, when washing with water to colourless and the apparent feminine gender of reducing sugar reaction earlier, reuse 20% ethanol is washed till colourless, uses 80% ethanol elution then, collect eluent, reclaim ethanol to the greatest extent, concentrate, be decompressed to-the 0.06Mpa drying, pulverizing, promptly get the Plumula Nelumbinis total alkali, measure through preceding method, containing the Plumula Nelumbinis total alkali is 64.4%.
The preparation method of the 9th kind of Plumula Nelumbinis total alkali of embodiment 9
Get Plumula Nelumbinis medical material 1kg, each 80% acidic ethanol reflux, extract, 4 times (1 hour/time) with 5 times of amounts, merge extractive liquid,, filter, filtrate is concentrated into does not have the alcohol flavor, and transfers concentrated solution volume to every ml medicinal liquid to be equivalent to the crude drugs of 2 grams, 10 ℃ of following cold preservation 12 hours, centrifugal filtration is got filtrate and is gone up macroporous adsorptive resins (AB-8); Behind the upper prop, make it abundant absorption, when washing with water to colourless and the apparent feminine gender of reducing sugar reaction earlier, reuse 15% ethanol is washed till colourless, uses 75% acidic ethanol eluting then, collect eluent, reclaim ethanol to the greatest extent, concentrate, constant pressure and dry, pulverizing, promptly get the Plumula Nelumbinis total alkali, measure through preceding method, containing the Plumula Nelumbinis total alkali is 61.8%.
Above content be in conjunction with concrete preferred implementation to further describing that the present invention did, can not assert that concrete enforcement of the present invention is confined to these explanations.For the general technical staff of the technical field of the invention, without departing from the inventive concept of the premise, its framework form can be flexible and changeable, can the subseries product.Just make some simple deduction or replace, all should be considered as belonging to the scope of patent protection that the present invention is determined by claims of being submitted to.
Claims (10)
1. the total alkali mixture of the Chinese crude drug Plumula Nelumbinis of originating is characterized in that: the content of Plumula Nelumbinis total alkali of three summations that comprises liensinine, isoliensinine, (-)-Neferine is more than 50%W/W.
2. the total alkali mixture of source as claimed in claim 1 Chinese crude drug Plumula Nelumbinis is characterized in that: wherein in extract gross weight liensinine content be not less than 5%-40%, isoliensinine content is not less than 1%-20% (-)-Neferine content and is not less than 5%-30%.
3. the extraction preparation method of the total alkali mixture of the Chinese crude drug Plumula Nelumbinis of originating is characterized in that: may further comprise the steps successively:
(1) acidic ethanol extraction
The Plumula Nelumbinis raw medicinal herbs is pulverized No. two of 2000 editions pharmacopeia regulation sieved into coarse powder, with the acidic ethanol reflux, extract, of 60%～80%V/V, pH=1-3 2～4 times, the acidic ethanol consumption is 4～6 times of Plumula Nelumbinis crude drug amount, and extraction time is 1～3 hour, and each time extracting solution is merged;
(2) filtering and concentrating
With extracting liquid filtering, be concentrated into and do not have the alcohol flavor, and with extracting solution be concentrated into every ml medicinal liquid be equivalent to 1 the gram～3 the gram the Plumula Nelumbinis raw medicinal herbs, 0 ℃～10 ℃ following cold preservations 10 hours～12 hours, be 4000～6000 rev/mins of following centrifugal filtrations 9 minutes～11 minutes with concentrated solution in speed again;
(3) macroporous resin adsorption
Concentrated solution filtrate after the centrifugal filtration is gone up the macroporous resin column adsorption and enrichment, obtain intermediate;
(4) wash post
Be washed till the intermediate that adsorbs colourless with big water gaging earlier, and continue again to wash to sugar-free, it is negative that reducing sugar reaction shows, reuse 10%～20%V/V low-concentration ethanol is washed till the intermediate of absorption colourless, remove a part of fat-soluble impurity, use the acidic ethanol eluting of 60%～80%V/V, pH=1-3 then, collect eluent, reclaim ethanol to most;
(5) concentrate
To be neutralized to pH=8-9 with the eluent that 60%～80%V/V acidic ethanol elutes and be concentrated into the thick paste that relative density is 1.15～1.25 (60 ℃);
(6) drying
Under 60 ℃～80 ℃, be dried to water content and be 4%～6% dried cream;
(7) pulverize, sieve, promptly.
4. the extraction preparation method of the total alkali mixture of source as claimed in claim 3 Chinese crude drug Plumula Nelumbinis is characterized in that: the aperture of used macroporous adsorptive resins is that 90A °～100A °, specific surface area are 500m
2/ g～550m
2/ g
5. as the extraction preparation method of the total alkali mixture of claim 3 or 4 described source Chinese crude drug Plumula Nelumbiniss, it is characterized in that: described step (1) is to be 70% (V/V) acidic ethanol reflux, extract, of 6 times 3 times of Plumula Nelumbinis crude drug amount with consumption, and extraction time is 2 hours.
6. according to the extraction preparation method of the total alkali mixture of claim 3 or 4 described source Chinese crude drug Plumula Nelumbiniss, it is characterized in that: described step (2) is that extracting solution is concentrated into the Plumula Nelumbinis raw medicinal herbs that every ml medicinal liquid is equivalent to 2 grams.
7. according to the extraction preparation method of the total alkali mixture of claim 3 or 4 described source Chinese crude drug Plumula Nelumbiniss, it is characterized in that: described step (2) is to be 5000 rev/mins of following centrifugal filtrations 10 minutes with concentrated solution in speed.
8. according to the extraction preparation method of the total alkali mixture of claim 3 or 4 described source Chinese crude drug Plumula Nelumbiniss, it is characterized in that: the used macroporous adsorptive resins of described step (3) is a nonpolarity macroporous adsorptive resins chromatographic column.
9. the extraction preparation method of the total alkali mixture of source according to claim 8 Chinese crude drug Plumula Nelumbinis is characterized in that: described nonpolarity macroporous adsorptive resins chromatographic column is D101 or AB-8 or NKA or HPD100, HPD300 or HPD100a or XAD-2 or HP20.
10. as the extraction preparation method of the total alkali mixture of claim 3 or 4 described source Chinese crude drug Plumula Nelumbiniss, it is characterized in that: described step (6) drying comprises constant pressure and dry, drying under reduced pressure, vacuum drying, spray drying, microwave drying, vacuum microwave drying.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114276293A (en) * | 2022-01-09 | 2022-04-05 | 福建中医药大学 | Preparation and purification method of neferine perchlorate |
| CN114349696A (en) * | 2022-01-09 | 2022-04-15 | 福建中医药大学 | Preparation and purification method of lotus nut total alkali perchlorate |
| CN114349698A (en) * | 2022-01-09 | 2022-04-15 | 福建中医药大学 | Preparation and purification method of liensinine perchlorate |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1257070A (en) * | 1998-12-14 | 2000-06-21 | 同济医科大学 | Process for extracting purified isoliensinine and liensinine from plumula nelumbinis |
-
2010
- 2010-04-08 CN CN 201010142202 patent/CN101791335A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1257070A (en) * | 1998-12-14 | 2000-06-21 | 同济医科大学 | Process for extracting purified isoliensinine and liensinine from plumula nelumbinis |
Non-Patent Citations (1)
| Title |
|---|
| 《中国实验方剂学杂志》 20070331 柳伟等 大孔吸附树脂纯化莲子心总生物碱的工艺研究 23-25 3-10 第13卷, 第3期 2 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114276293A (en) * | 2022-01-09 | 2022-04-05 | 福建中医药大学 | Preparation and purification method of neferine perchlorate |
| CN114349696A (en) * | 2022-01-09 | 2022-04-15 | 福建中医药大学 | Preparation and purification method of lotus nut total alkali perchlorate |
| CN114349698A (en) * | 2022-01-09 | 2022-04-15 | 福建中医药大学 | Preparation and purification method of liensinine perchlorate |
| CN114349696B (en) * | 2022-01-09 | 2023-10-03 | 福建中医药大学 | Preparation and purification method of lotus nut total alkali perchlorate |
| CN114349698B (en) * | 2022-01-09 | 2024-01-26 | 福建中医药大学 | Preparation and purification method of liensinine perchlorate |
| CN114276293B (en) * | 2022-01-09 | 2024-01-26 | 福建中医药大学 | Preparation and purification method of methyl lotus plumule perchlorate |
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