CN106478578A - A kind of method of physical field assisted extraction Exocarpium Litchi procyanidin - Google Patents
A kind of method of physical field assisted extraction Exocarpium Litchi procyanidin Download PDFInfo
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- CN106478578A CN106478578A CN201610751602.XA CN201610751602A CN106478578A CN 106478578 A CN106478578 A CN 106478578A CN 201610751602 A CN201610751602 A CN 201610751602A CN 106478578 A CN106478578 A CN 106478578A
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- procyanidin
- litchi
- assisted extraction
- physical field
- extraction
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/58—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4
- C07D311/60—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2
- C07D311/62—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2 with oxygen atoms directly attached in position 3, e.g. anthocyanidins
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
- Extraction Or Liquid Replacement (AREA)
Abstract
The invention discloses a kind of method of physical field assisted extraction Exocarpium Litchi procyanidin, comprise the following steps：（1）After Fructus Litchi shell dries, low-temperature grinding, sieve；（2）Fructus Litchi shell after sieving adds in ethanol solution, first carries out microwave and supersonic synergic is processed, then carry out impulse electric field process, then extract；（3）Carry out eluting process with macroporous adsorbent resin again, eluent is carried out with concentrated in vacuo, laser treatment, last lyophilization must be rich in the powder of procyanidin.The method extracting procyanidin from Fructus Litchi shell that the present invention provides, the general ethanol extraction technology of ratio, the extraction ratio of procyanidin improves 50% ~ 70%, the ability that the procyanidin extracting removes DPPH free radical improves 30% 62%, the in vitro anti-oxidation of procyanidin is higher, and whole extraction process is efficient, pollution-free, inexpensive.
Description
Technical field
The present invention relates to fruit deep process technology field, a kind of more particularly to physical field assisted extraction Exocarpium Litchi is former
The method of anthocyanidin.
Background technology
In industrialized production, a large amount of Fructus Litchi shells abandon Lingnan features fruit Fructus Litchi as garbage, Exocarpium Litchi in fact
In be rich in bioactive ingredients.CN201110339166.2 discloses a kind of litchi shell extract and preparation method thereof and in volume
Application in cigarette, is flowed back with petroleum ether first and extracts Fructus Litchi shell powder, collects the solid residue after backflow extraction, it is suitable to determine
Enzymatic hydrolysis condition, is digested and is further purified to carrying residue at the beginning of Fructus Litchi shell, prepared litchi shell extract is added to volume
Fragrance and jealous flavouring upgrading, fall thorn remove impurity and the reduction that there is good matching, contribute to Medicated cigarette after in cigarette, with Medicated cigarette
The burst size of harmful components, the lifting to Medicated cigarette integrated quality has certain facilitation.CN201410180985.0 discloses one
Plant the preparation method of high-purity phloretin, with litchi rind as raw material, prepare litchi rind with high-purity weak polar solvent heating and extract
Liquid, gained extracting solution contains aqueous two-phase extraction through liquid-liquid extraction, and membrane technology separation etc. separates, purification process step, obtains final product high-purity
Degree phloretin.
The active component as procyanidin is also contained in Fructus Litchi shell.Procyanidin, abbreviation OPC, it is special to be that one kind has
The bioflavonoids of molecular structure, are the maximally effective Natural antioxidants of removing people's interior free yl generally acknowledged in the world at present,
Have and improve blood circulation, protection vision, promote cholesterol decomposition, cardioprotection function, antiallergic inflammation and skin is had
The functions such as crease-resistant, convergence, moisturizing, health care is good.CN201210009310.0 discloses a kind of bark of dahurian larch procyanidin
Extracting method, it comprises the steps：(1) bark of dahurian larch is ground into graininess；(2) extracted with super critical extraction, obtain
Procyanidin extracting solution, this invention makes full use of the bark of dahurian larch in Daxing'an Mountainrange, have raw material be easy to get, low cost, supercritical
Extractive technique yield is high, extraction of substance is not damaged, production safety environmental protection the features such as, order can be obtained to the demand of procyanidin
The satisfied result of people；A kind of open inexpensive, efficient, the free of contamination procyanidin extraction process of CN201110211044.5, all
It is not directed to a kind of method of physical field assisted extraction Exocarpium Litchi procyanidin.
Content of the invention
The technical problem to be solved there is provided a kind of side of physical field assisted extraction Exocarpium Litchi procyanidin
Method.
The technical problem to be solved is achieved by the following technical programs：
A kind of method of physical field assisted extraction Exocarpium Litchi procyanidin, comprises the following steps：
（1）After Fructus Litchi shell dries, low-temperature grinding, sieve；
（2）Fructus Litchi shell after sieving adds in ethanol solution, first carries out microwave and supersonic synergic is processed, then enter horizontal pulse electricity
Field is processed, and then extracts；
（3）Carry out eluting process with macroporous adsorbent resin again, concentrated in vacuo, laser treatment is carried out to eluent, finally freeze dry
The dry powder that must be rich in procyanidin.
In the present invention, described Fructus Litchi shell adds in ethanol solution and is specially with solid-liquid ratio for 1:6 ~ 9 ratio was incited somebody to action
Fructus Litchi shell after sieve adds in 60% ~ 80% ethanol solution.
In the present invention, described collaborative treatment conditions are supersonic frequency 20 ~ 24kHz, process time 10 ~ 20min.
In the present invention, described impulse electric field treatment conditions for frequency be 10Hz, electric field intensity be 30-40kV/cm, process
Time 300 ~ 600 μ s.
In the present invention, described eluting processes and adopts 10 ~ 15 times of distilled water and 40% ~ 50% ethanol elution.
In the present invention, described temperature control concentrated in vacuo is at 35 ~ 50 DEG C.
In the present invention, described extraction specially extracts 30 ~ 42min again under conditions of 40 ~ 50 DEG C.
In the present invention, the described laser treatment time is 30 ~ 60s.
The present invention has the advantages that：
The method extracting procyanidin from Fructus Litchi shell that the present invention provides, the general ethanol extraction technology of ratio, procyanidin
Extraction ratio improves 50% ~ 70%, and the ability of the procyanidin removing DPPH free radical of extraction improves 30%-62%, procyanidin
In vitro anti-oxidation higher, whole extraction process is efficient, pollution-free, inexpensive.
Specific embodiment
With reference to embodiment, the present invention will be described in detail, and embodiment is only the preferred embodiment of the present invention,
It is not limitation of the invention.
Embodiment 1
（1）After Fructus Litchi shell dries naturally, low-temperature grinding, cross 60 mesh sieves；
（2）With solid-liquid ratio for 1:6 ratio adds 60%（Volume content）Ethanol solution, the ultrasonic association of microwave and frequency 20kHz
With processing 10min, then using frequency be 10Hz, electric field intensity be the impulse electric field of 30kV/cm, process time 300 μ s, then 40
30min is extracted under conditions of DEG C；
（3）Use macroporous adsorbent resin again, using 10 times of distilled water and 40%（Volume content）Ethanol elution, eluent is true at 35 DEG C
Empty concentration, lyophilization after laser treatment 30s must be rich in the powder of procyanidin.
The method extracting procyanidin from Fructus Litchi shell that the present invention provides, the general ethanol extraction technology of ratio, former cyanine
The extraction ratio of element improves 50%, and the ability of the procyanidin removing DPPH free radical of extraction improves 30%, the body of procyanidin
Outer non-oxidizability is higher, and whole extraction process is efficient, pollution-free, inexpensive.
Embodiment 2
（1）After Fructus Litchi shell dries naturally, low-temperature grinding, cross 80 mesh sieves；
（2）With solid-liquid ratio for 1:8 ratio adds 70%（Volume content）Ethanol solution, the ultrasonic association of microwave and frequency 22kHz
With processing 15min, then using frequency be 10Hz, electric field intensity be the impulse electric field of 35kV/cm, process time 400 μ s, then 45
35min is extracted under conditions of DEG C；
（3）Use macroporous adsorbent resin again, using 13 times of distilled water and 45%（Volume content）Ethanol elution, eluent is true at 40 DEG C
Empty concentration, lyophilization after laser treatment 40s must be rich in the powder of procyanidin.
The method extracting procyanidin from Fructus Litchi shell that the present invention provides, the general ethanol extraction technology of ratio, former cyanine
The extraction ratio of element improves 60%, and the ability of the procyanidin removing DPPH free radical of extraction improves 42%, the body of procyanidin
Outer non-oxidizability is higher, and whole extraction process is efficient, pollution-free, inexpensive.
Embodiment 3
（1）After Fructus Litchi shell dries naturally, low-temperature grinding, cross 100 mesh sieves；
（2）With solid-liquid ratio for 1:9 ratio adds 80%（Volume content）Ethanol solution, the ultrasonic association of microwave and frequency 24kHz
With processing 20min, then using frequency be 10Hz, electric field intensity be the impulse electric field of 40kV/cm, process time 600 μ s, then 50
42min is extracted under conditions of DEG C；
（3）Use macroporous adsorbent resin again, using 15 times of distilled water and 50%（Volume content）Ethanol elution, eluent is true at 50 DEG C
Empty concentration, lyophilization after laser treatment 60s must be rich in the powder of procyanidin.
The method extracting procyanidin from Fructus Litchi shell that the present invention provides, the general ethanol extraction technology of ratio, former cyanine
The extraction ratio of element improves 70%, and the ability of the procyanidin removing DPPH free radical of extraction improves 62%, the body of procyanidin
Outer non-oxidizability is higher, and whole extraction process is efficient, pollution-free, inexpensive.
Embodiment described above only have expressed embodiments of the present invention, and its description is more concrete and detailed, but can not
Therefore it is interpreted as the restriction to the scope of the claims of the present invention, as long as the skill being obtained in the form of equivalent or equivalent transformation
Art scheme, all should fall within the scope and spirit of the invention.
Claims (8)
1. a kind of method of physical field assisted extraction Exocarpium Litchi procyanidin, comprises the following steps：
（1）After Fructus Litchi shell dries, low-temperature grinding, sieve；
（2）Fructus Litchi shell after sieving adds in ethanol solution, first carries out microwave and supersonic synergic is processed, then enter horizontal pulse electricity
Field is processed, and then extracts；
（3）Carry out eluting process with macroporous adsorbent resin again, concentrated in vacuo, laser treatment is carried out to eluent, finally freeze dry
The dry powder that must be rich in procyanidin.
2. the method for physical field assisted extraction Exocarpium Litchi procyanidin according to claim 1 is it is characterised in that described litchi
Branch shell adds in ethanol solution specially with solid-liquid ratio for 1:Fructus Litchi shell after 6 ~ 9 ratio will be sieved adds 60% ~ 80%
Ethanol solution in.
3. the method for physical field assisted extraction Exocarpium Litchi procyanidin according to claim 1 and 2 is it is characterised in that institute
Stating collaborative treatment conditions is supersonic frequency 20 ~ 24kHz, process time 10 ~ 20min.
4. the method for physical field assisted extraction Exocarpium Litchi procyanidin according to claim 3 is it is characterised in that described arteries and veins
Rush electric field treatment condition for frequency be 10Hz, electric field intensity be 30-40kV/cm, process time 300 ~ 600 μ s.
5. the method for physical field assisted extraction Exocarpium Litchi procyanidin according to claim 4 is it is characterised in that described wash
De- process adopts 10 ~ 15 times of distilled water and 40% ~ 50% ethanol elution.
6. the method for physical field assisted extraction Exocarpium Litchi procyanidin according to claim 5 is it is characterised in that described carry
Take and specially extract 30 ~ 42min again under conditions of 40 ~ 50 DEG C.
7. according to claim 1 or 5 the method for physical field assisted extraction Exocarpium Litchi procyanidin it is characterised in that institute
State temperature control concentrated in vacuo at 35 ~ 50 DEG C.
8. the method for physical field assisted extraction Exocarpium Litchi procyanidin according to claim 1 is it is characterised in that described swash
The optical processing time is 30 ~ 60s.
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| CN201610751602.XA CN106478578A (en) | 2016-08-30 | 2016-08-30 | A kind of method of physical field assisted extraction Exocarpium Litchi procyanidin |
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| CN201610751602.XA CN106478578A (en) | 2016-08-30 | 2016-08-30 | A kind of method of physical field assisted extraction Exocarpium Litchi procyanidin |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107227233A (en) * | 2017-07-31 | 2017-10-03 | 广西驰胜农业科技有限公司 | A kind of lichee fruit wine brewing method of use microwave radiation |
| CN107227227A (en) * | 2017-07-07 | 2017-10-03 | 广西驰胜农业科技有限公司 | A kind of preparation method of lichee fruit wine |
| CN107227228A (en) * | 2017-07-18 | 2017-10-03 | 广西驰胜农业科技有限公司 | A kind of use amino acid regulating and controlling fermentation, the lichee fruit wine preparation method of ageing |
| CN107279236A (en) * | 2017-06-30 | 2017-10-24 | 武汉轻工大学 | A kind of low AGEs inulin cookies and preparation method thereof |
| CN108452006A (en) * | 2018-06-01 | 2018-08-28 | 台州学院 | A kind of method of impulse electric field-aqueous two-phase-column chromatography combination extraction plant polyphenol |
| CN109111364A (en) * | 2017-06-22 | 2019-01-01 | 中国烟草总公司广东省公司 | A kind of method of high-pressure pulse electric combination microwave, ultrasonic wave extraction tobacco waste Content of Chlorogenic Acid |
| CN114524793A (en) * | 2022-01-25 | 2022-05-24 | 河南农业大学 | Method for extracting and purifying procyanidine from peanut skin |
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| CN104557838A (en) * | 2014-12-11 | 2015-04-29 | 惠州学院 | Method for extracting proantho cyanidins from litchi peels |
| CN105294636A (en) * | 2015-11-16 | 2016-02-03 | 大兴安岭林格贝寒带生物科技股份有限公司 | Preparation method of oligomeric proanthocyanidin |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109111364A (en) * | 2017-06-22 | 2019-01-01 | 中国烟草总公司广东省公司 | A kind of method of high-pressure pulse electric combination microwave, ultrasonic wave extraction tobacco waste Content of Chlorogenic Acid |
| CN109111364B (en) * | 2017-06-22 | 2021-06-22 | 中国烟草总公司广东省公司 | Method for extracting chlorogenic acid from tobacco waste by combining high-voltage pulsed electric field with microwaves and ultrasonic waves |
| CN107279236A (en) * | 2017-06-30 | 2017-10-24 | 武汉轻工大学 | A kind of low AGEs inulin cookies and preparation method thereof |
| CN107227227A (en) * | 2017-07-07 | 2017-10-03 | 广西驰胜农业科技有限公司 | A kind of preparation method of lichee fruit wine |
| CN107227228A (en) * | 2017-07-18 | 2017-10-03 | 广西驰胜农业科技有限公司 | A kind of use amino acid regulating and controlling fermentation, the lichee fruit wine preparation method of ageing |
| CN107227233A (en) * | 2017-07-31 | 2017-10-03 | 广西驰胜农业科技有限公司 | A kind of lichee fruit wine brewing method of use microwave radiation |
| CN108452006A (en) * | 2018-06-01 | 2018-08-28 | 台州学院 | A kind of method of impulse electric field-aqueous two-phase-column chromatography combination extraction plant polyphenol |
| CN114524793A (en) * | 2022-01-25 | 2022-05-24 | 河南农业大学 | Method for extracting and purifying procyanidine from peanut skin |
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Application publication date: 20170308 |