CN105294325A - Leaf fertilizer containing natural high-activity iturins sodium surfactin and preparation method of leaf fertilizer - Google Patents
Leaf fertilizer containing natural high-activity iturins sodium surfactin and preparation method of leaf fertilizer Download PDFInfo
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- CN105294325A CN105294325A CN201510891480.XA CN201510891480A CN105294325A CN 105294325 A CN105294325 A CN 105294325A CN 201510891480 A CN201510891480 A CN 201510891480A CN 105294325 A CN105294325 A CN 105294325A
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Abstract
The invention belongs to the field of agricultural fertilizers and discloses a leaf fertilizer containing natural high-activity iturins sodium surfactin and a preparation method of the leaf fertilizer. The iturins sodium surfactin is capable of improving the permeability of cells and the disease resistance of crops, promoting the normal growth of the crops and improving the nutrient absorption of the crops. The leaf fertilizer contains the iturins sodium surfactin, so that the nutrient absorption of the crops is improved, the biomass of the crops is increased; meanwhile, the iturins sodium surfactin can be absorbed and utilized by the crops, has high possibility of being environmentally degraded and is environmentally friendly. The preparation method of the leaf fertilizer is simple in operation and applicable to industrial production.
Description
Technical field
The invention belongs to agricultural fertilizer field, be specifically related to a kind of foliage fertilizer, especially relate to a kind of containing the foliage fertilizer of sodium bacillus subtilis lipopeptide and the preparation method of this foliage fertilizer.
Background technology
In agriculture production, the development of plant husbandry be unable to do without fertilizer.Fertilizer is to provide the required mineral element of one or more plants, improves soil property, a class material of level of increasing soil fertility, and is one of basic substance of agriculture production.In recent years along with the development of fertilizer practice, leaf dressing, as strengthening the nutrition of crop and preventing a kind of measures of fertilizer of some nutritional deficiency symptom, has obtained rapid promotion and application.Facts have proved, compared with soil application, leaf dressing is the technology various nutrient needed for crop directly being imposed on blade face, has that nutrition absorption is fast, absorption rate high.Along with the development of intensive agricultural, leaf dressing has become an important fertilizer practice measure.
Nutrient on blade face mainly through blade face the expolasm of pore, the cuticular hydrophilic aperture of leaf surface and blade cell connect silk etc. and enter mesophyll cell by blade epidermis.But leaf dressing also exists some problems (1) nutrient in the application, and to enter the efficiency of mesophyll tissue lower, the crop that particularly blade stratum corneum is thicker; (2) due to the hydrophobicity of blade surface, the nutrient spraying application to blade surface, easily from blade face drippage, causes waste.
At present, in order to improve the absorption of crop to nutrient, in foliage fertilizer, add auxiliary agent more.As application number 201210206014X, denomination of invention is the Chinese patent of a kind of multifunctional paddy volume increase foliage fertilizer and preparation method thereof, nonionogenic tenside castor oil polyoxyethylene ether is added in foliage fertilizer disclosed in this patent, addition is 5-6%, but this Solubility of Substances is very low, production process efficiency is not high.Application number is 201310231728.0, denomination of invention is that a kind of preparation method of selenium-enriched foliage fertilizer and the Chinese patent of application disclose in foliage fertilizer and add anion surfactant sodium laurylsulfonate (SDS), addition is 0.5-1.5%, but SDS is not easily biodegradable at occurring in nature, there is certain pollution to environment.Application number 201510221601.X, denomination of invention is disclose in foliage fertilizer containing the Chinese patent of alkaloid paddy rice humic acid foliage fertilizer and preparation method thereof to add aliphatics Volpo S 10, addition is 20-30%, and a large amount of aliphatics Volpo S 10s certainly will to environment.
Summary of the invention
In view of this, the present invention is directed to the deficiencies in the prior art, a kind of foliage fertilizer and preparation method thereof is provided.Foliage fertilizer of the present invention can not only improve the absorption of crop to nutrient, promotes plant growth, and environmentally safe.
For realizing object of the present invention, the present invention adopts following technical scheme:
A kind of foliage fertilizer, comprises sodium bacillus subtilis lipopeptide.
Preferably, the content of described sodium bacillus subtilis lipopeptide is 20g/L ~ 50g/L.
In some embodiments, described foliage fertilizer is made up of the raw material of following weight part: 8 ~ 14 parts, urea, three hypophosphite monohydrate hydrogen dipotassiums 12 ~ 18 parts, 8 ~ 10 parts, saltpetre, sodium tetraborate 0.8 ~ 1.2 part, ammonium molybdate 0.4 ~ 0.6 part, compound amino acid powder 10 ~ 20 parts, potassium humate 4 ~ 6 parts, sodium bacillus subtilis lipopeptide 3 ~ 5 parts, 60 ~ 80 parts, water.
Present invention also offers a kind of preparation method of foliage fertilizer, by weight, take 10 ~ 20 parts of compound amino acid powders and to add water middle stirring and dissolving; Add 8 ~ 10 parts of saltpetre, 8 ~ 14 parts of urea, 12 ~ 18 part of three hypophosphite monohydrate hydrogen dipotassium, 0.4 ~ 0.6 part of ammonium molybdate, 0.8 ~ 1.2 part of sodium tetraborate stirring and dissolving successively, obtain settled solution; Add 3 ~ 5 parts of sodium bacillus subtilis lipopeptide stirring and dissolving, add 4 ~ 6 parts of sodium humates and dissolve.
Present invention also offers sodium bacillus subtilis lipopeptide and improve crop to the purposes of the absorption of nutrient.
Present invention also offers the purposes that sodium bacillus subtilis lipopeptide improves membrane passage.
Present invention also offers the purposes that sodium bacillus subtilis lipopeptide improves the disease resistance of crop.
As shown from the above technical solution, the invention provides the novelty teabag of sodium bacillus subtilis lipopeptide and comprise the foliage fertilizer of sodium bacillus subtilis lipopeptide.Sodium bacillus subtilis lipopeptide can improve the disease resistance of membrane passage and crop, promotes the normal growth of crop, and then improves crop to the absorption of nutrient.Foliage fertilizer of the present invention comprises sodium bacillus subtilis lipopeptide, improves crop to the absorption of nutrient, improves crop biomass, simultaneously the utilization absorbed by crops of sodium bacillus subtilis lipopeptide energy, and easily by environment degradable, environmentally safe.The preparation method of foliage fertilizer of the present invention is simple to operate, is suitable for suitability for industrialized production.
Embodiment
Below in conjunction with the embodiment of the present invention, be clearly and completely described the technical scheme in the embodiment of the present invention, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
Specific conductivity is larger, and permeability is larger.The present invention utilizes different concns sodium bacillus subtilis lipopeptide process Plantula Brassicae chinensis blade 20 minutes, measures the specific conductivity of blade.The permeability of result display blade increases along with surfaction na concn and increases.Show that sodium bacillus subtilis lipopeptide can improve membrane passage.Therefore the invention provides the purposes that sodium bacillus subtilis lipopeptide improves membrane passage.
The defensive ability/resistance ability of plant to poor environment is commonly used anti-oxidant activity and is characterized.The present invention utilizes different concns surfaction sodium solution to spray Plantula Brassicae chinensis blade, after 30 minutes, cut blade, measure antiperoxidase and the catalase specific conductivity of blade, the interpolation of result display sodium bacillus subtilis lipopeptide effectively can improve the resistance of oxidation of Plantula Brassicae chinensis, thus be conducive to improving its defence capability, strengthen its disease resistance.Therefore the invention provides the purposes that sodium bacillus subtilis lipopeptide improves the disease resistance of crop.
Sodium bacillus subtilis lipopeptide can improve the disease resistance of membrane passage and crop, promotes the normal growth of crop, and then improves crop to the absorption of nutrient.Therefore the invention provides sodium bacillus subtilis lipopeptide and improve crop to the purposes of the absorption of nutrient
Present invention also offers a kind of foliage fertilizer, comprise sodium bacillus subtilis lipopeptide.
Foliage fertilizer of the present invention comprises sodium bacillus subtilis lipopeptide, contribute to opening of blade face pore, blade face liquid-drop contact angle can be reduced simultaneously, greatly increase wetting areas, have additional nutrients in the residence time on blade face, thus be conducive to Foliage Absorption nutrition, improve crop to the purposes of the absorption of nutrient.Sodium bacillus subtilis lipopeptide in foliage fertilizer meet micella that water formed can effectively by the Nutrient absorption in foliage fertilizer, adhere to plant leaf surface; prevent the nutrient loss because rain drop erosion and Exposure to Sunlight volatilization are formed; micella can pin moisture simultaneously, has good provide protection to crop in high temperature, arid.Sodium bacillus subtilis lipopeptide is cyclic lipopeptide anionic natural biological Surfactin, can adsorb nutrient ion in nutrient, and the nutrient in foliage fertilizer is transported to quickly through portion's conduit the position that plant needs, and giving plant provides and need nutrient.Nutrient, moisture can pin at plant surface by the foliage fertilizer comprising sodium bacillus subtilis lipopeptide, spreadability is good, strong adhesion, energy long time retaining water content, nutrient, absorbed nutrient energy fast transfer simultaneously, should not cause at plant interior accumulation, cause waste, improve plant recovery of nutrient, save cost.
Preferably, the content of described sodium bacillus subtilis lipopeptide is 20g/L ~ 50g/L.
Wherein, described sodium bacillus subtilis lipopeptide is the sodium salt of surfaction, be subtilis be the cyclic lipopeptide compound of 1022DA by the main molecules amount that biological fermentation process is produced, by seven amino acid (2 L-Leu, 2 D-Leu, 1 L-Glu, 1 L-Asp, 1 L-Val) and 1 long-chain beta-hydroxy fatty acid composition.
In some embodiments, foliage fertilizer of the present invention, is made up of the raw material of following weight part: 8 ~ 14 parts, urea, three hypophosphite monohydrate hydrogen dipotassiums 12 ~ 18 parts, 8 ~ 10 parts, saltpetre, sodium tetraborate 0.8 ~ 1.2 part, ammonium molybdate 0.4 ~ 0.6 part, compound amino acid powder 10 ~ 20 parts, potassium humate 4 ~ 6 parts, sodium bacillus subtilis lipopeptide 3 ~ 5 parts, 60 ~ 80 parts, water.
In certain embodiments, foliage fertilizer of the present invention, is made up of the raw material of following weight part: 8 parts, urea, three hypophosphite monohydrate hydrogen dipotassiums 12 parts, 10 parts, saltpetre, sodium tetraborate 0.8 part, ammonium molybdate 0.6 part, compound amino acid powder 20 parts, potassium humate 6 parts, sodium bacillus subtilis lipopeptide 3 parts, 80 parts, water.
In certain embodiments, foliage fertilizer of the present invention, is made up of the raw material of following weight part: 14 parts, urea, three hypophosphite monohydrate hydrogen dipotassiums 18 parts, 8 parts, saltpetre, sodium tetraborate 1.2 parts, ammonium molybdate 0.4 part, compound amino acid powder 10 parts, potassium humate 4 parts, sodium bacillus subtilis lipopeptide 5 parts, 60 parts, water.
In certain embodiments, foliage fertilizer of the present invention, is made up of the raw material of following weight part: 12 parts, urea, three hypophosphite monohydrate hydrogen dipotassiums 15 parts, 9 parts, saltpetre, sodium tetraborate 1.0 parts, ammonium molybdate 0.5 part, compound amino acid powder 15 parts, potassium humate 5 parts, sodium bacillus subtilis lipopeptide 4 parts, 70 parts, water.
In other specific embodiments, foliage fertilizer of the present invention, is made up of the raw material of following weight part: 10 parts, urea, three hypophosphite monohydrate hydrogen dipotassiums 14 parts, 10 parts, saltpetre, sodium tetraborate 1.0 parts, ammonium molybdate 0.4 part, compound amino acid powder 20 parts, potassium humate 4 parts, sodium bacillus subtilis lipopeptide 4 parts, 80 parts, water.Weight part of the present invention, can be weight unit well known to those skilled in the art, such as gram, kilogram, kilogram, ton etc.
Compound amino acid powder of the present invention is with people or animal hair, fur or with dregs of beans, the dish dregs of rice, cotton dregs are raw material, after fermentable, acid and alkali hydrolysis process, spraying dry processes, and its Gelucystine be rich in, tyrosine, Serine animal proteinum are through acidolysis, neutralization, concentrated, dry obtained.Can be obtained from purchase by commercial sources.
Present invention also offers a kind of preparation method of foliage fertilizer.
The preparation method of foliage fertilizer of the present invention, by weight, takes 10 ~ 20 parts of compound amino acid powders and to add water middle stirring and dissolving; Add 8 ~ 10 parts of saltpetre, 8 ~ 14 parts of urea, 12 ~ 18 part of three hypophosphite monohydrate hydrogen dipotassium, 0.4 ~ 0.6 part of ammonium molybdate, 0.8 ~ 1.2 part of sodium tetraborate stirring and dissolving successively, obtain settled solution; Add 3 ~ 5 parts of sodium bacillus subtilis lipopeptide stirring and dissolving, add 4 ~ 6 parts of sodium humates and dissolve.
Below in conjunction with specific embodiment, the present invention is described in further detail.Wherein if no special instructions sodium bacillus subtilis lipopeptide of the present invention all purchased from Anhui Province Diyuan Biotechnology CO.,Ltd.
Embodiment 1: blade face permeability is tested
Specific conductivity is larger, and permeability is larger.Utilize different concns sodium bacillus subtilis lipopeptide process Plantula Brassicae chinensis blade 20 minutes, measure the specific conductivity of blade, result is as table 1.
Table 1 Conductivity Results
From table 1 result, the permeability of blade increases along with surfaction na concn and increases.
Embodiment 2: resistance against diseases
Utilize different concns surfaction sodium solution to spray Plantula Brassicae chinensis blade, after 30 minutes, cut blade, measure peroxidase and the catalase specific conductivity of blade, result is as table 2.
Table 2 resistance of oxidation result
From table 2 result, the interpolation of sodium bacillus subtilis lipopeptide effectively can improve the resistance of oxidation of Plantula Brassicae chinensis, thus is conducive to improving its defence capability, strengthens its disease resistance.
Embodiment 3:
Formula: urea 8g, three hypophosphite monohydrate hydrogen dipotassium 12g, saltpetre 10g, sodium tetraborate 0.8g, ammonium molybdate 0.6g, compound amino acid powder 20g, potassium humate 6g, sodium bacillus subtilis lipopeptide 3g, water 80g.
Preparation method: add water compound amino acid powder stirring and dissolving, if there is a small amount of insolubles, crosses and filters; Add saltpetre, urea, three hypophosphite monohydrate hydrogen dipotassiums, ammonium molybdate, sodium tetraborate successively, stirring and dissolving; After adding sodium bacillus subtilis lipopeptide stirring and dissolving, after adding sodium humate stirring and dissolving, obtain foliage fertilizer finished product.Use doubly rear for this product dilution 100-200 before spraying.
Embodiment 4:
Formula: urea 14g, three hypophosphite monohydrate hydrogen dipotassium 18g, saltpetre 8g, sodium tetraborate 1.2g, ammonium molybdate 0.4g, compound amino acid powder 10g, potassium humate 4g, sodium bacillus subtilis lipopeptide 5g, water 60g.
Preparation method: add water compound amino acid powder stirring and dissolving, if there is a small amount of insolubles, crosses and filters; Add saltpetre, urea, three hypophosphite monohydrate hydrogen dipotassiums, ammonium molybdate, sodium tetraborate successively, stirring and dissolving; After adding sodium bacillus subtilis lipopeptide stirring and dissolving, after adding sodium humate stirring and dissolving, obtain foliage fertilizer finished product.Use doubly rear for this product dilution 100-200 before spraying.
Embodiment 5:
Formula: urea 12g, three hypophosphite monohydrate hydrogen dipotassium 15g, saltpetre 9g, sodium tetraborate 1.0g, ammonium molybdate 0.5g, compound amino acid powder 15g, potassium humate 5g, sodium bacillus subtilis lipopeptide 4g, water 70g.
Preparation method: add water compound amino acid powder stirring and dissolving, if there is a small amount of insolubles, crosses and filters; Add saltpetre, urea, three hypophosphite monohydrate hydrogen dipotassiums, ammonium molybdate, sodium tetraborate successively, stirring and dissolving; After adding sodium bacillus subtilis lipopeptide stirring and dissolving, after adding sodium humate stirring and dissolving, obtain foliage fertilizer finished product.
Use doubly rear for this product dilution 100-200 before spraying.
Embodiment 6:
Formula: urea 10g, three hypophosphite monohydrate hydrogen dipotassium 14g, saltpetre 10g, sodium tetraborate 1.0g, ammonium molybdate 0.4g, compound amino acid powder 20g, potassium humate 4g, sodium bacillus subtilis lipopeptide 4g, water 80g.
Obtained foliage fertilizer finished product is carried out by preparation method in embodiment 3.Use doubly rear for this product dilution 100-200 before spraying.
Embodiment 7:
After foliage fertilizer described in embodiment 3 is diluted 200 times, experiment is sprayed to potted plant Plantula Brassicae chinensis.Spray when Plantula Brassicae chinensis grows 4 true leaf, within every 7 days, spray 1 time, spray 4 times, and with spray the water gaging equivalent formulation groups such as sodium bacillus subtilis lipopeptide and not add and the tween 80 of sodium bacillus subtilis lipopeptide equivalent, polysorbas20, alkyl compare according to the equivalent formulation group foliar nutrient of the tensio-active agents such as oxygen Vinyl Ether (PPJ), sodium laurylsulfonate (SDS) containing sodium bacillus subtilis lipopeptide, respectively.When mensuration spraying concentration is 0.1g/L, spray the average biomass of individual tree of Plantula Brassicae chinensis (dry weight), result is as table 3.
Table 3 biomass compares
| Tensio-active agent | Average biomass of individual tree (gram) |
| Water | 0.234 |
| Sodium bacillus subtilis lipopeptide | 0.362 |
| Polysorbas20 | 0.285 |
| Tween 80 | 0.305 |
| PPJ | 0.325 |
| SDS | 0.218 |
The foliage fertilizer effect that sodium bacillus subtilis lipopeptide is added in result display is best, and the foliage fertilizer biomass more not containing sodium bacillus subtilis lipopeptide increases by 54.7%, comparatively adds foliage fertilizer volume increase about the 11%-35% of other tensio-active agents.
According to the method described above, spray experiment respectively to the foliage fertilizer described in embodiment 4-6, result is similar to the foliage fertilizer described in embodiment 3, and the foliage fertilizer effect of adding sodium bacillus subtilis lipopeptide is best.
Above content is only citing made for the present invention and explanation; affiliated those skilled in the art make various amendment to described specific embodiment or supplement or adopt similar mode to substitute; only otherwise depart from invention or surmount this scope as defined in the claims, protection scope of the present invention all should be belonged to.
Claims (7)
1. a foliage fertilizer, is characterized in that, comprises sodium bacillus subtilis lipopeptide.
2. foliage fertilizer according to claim 1, is characterized in that, the content of described sodium bacillus subtilis lipopeptide is 20g/L ~ 50g/L.
3. foliage fertilizer according to claim 1 and 2, it is characterized in that, be made up of the raw material of following weight part: 8 ~ 14 parts, urea, three hypophosphite monohydrate hydrogen dipotassiums 12 ~ 18 parts, 8 ~ 10 parts, saltpetre, sodium tetraborate 0.8 ~ 1.2 part, ammonium molybdate 0.4 ~ 0.6 part, compound amino acid powder 10 ~ 20 parts, potassium humate 4 ~ 6 parts, sodium bacillus subtilis lipopeptide 3 ~ 5 parts, 60 ~ 80 parts, water.
4. a preparation method for foliage fertilizer, is characterized in that, by weight, takes 10 ~ 20 parts of compound amino acid powders and to add water middle stirring and dissolving; Add 8 ~ 10 parts of saltpetre, 8 ~ 14 parts of urea, 12 ~ 18 part of three hypophosphite monohydrate hydrogen dipotassium, 0.4 ~ 0.6 part of ammonium molybdate, 0.8 ~ 1.2 part of sodium tetraborate stirring and dissolving successively, obtain settled solution; Add 3 ~ 5 parts of sodium bacillus subtilis lipopeptide stirring and dissolving, add 4 ~ 6 parts of sodium humates and dissolve.
5. sodium bacillus subtilis lipopeptide improves crop to the purposes of the absorption of nutrient.
6. sodium bacillus subtilis lipopeptide improves the purposes of membrane passage.
7. sodium bacillus subtilis lipopeptide improves the purposes of the disease resistance of crop.
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| CN108293990A (en) * | 2018-03-14 | 2018-07-20 | 安徽农业大学 | A kind of pesticide spray special synergistic agent and the preparation method and application thereof |
| CN112209762A (en) * | 2019-07-12 | 2021-01-12 | 山东金必来生物科技有限公司 | Macroelement water-soluble fertilizer containing microbial metabolite |
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Cited By (4)
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| CN107124933A (en) * | 2017-05-18 | 2017-09-05 | 安徽省农业科学院园艺研究所 | A kind of aspermous watermelon exempts from broken shell batch seeding growing seedlings method |
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| CN112209762A (en) * | 2019-07-12 | 2021-01-12 | 山东金必来生物科技有限公司 | Macroelement water-soluble fertilizer containing microbial metabolite |
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Application publication date: 20160203 |