CN105273991B - 用于在纳米孔中操作分子的系统和方法 - Google Patents
用于在纳米孔中操作分子的系统和方法 Download PDFInfo
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- CN105273991B CN105273991B CN201510614358.8A CN201510614358A CN105273991B CN 105273991 B CN105273991 B CN 105273991B CN 201510614358 A CN201510614358 A CN 201510614358A CN 105273991 B CN105273991 B CN 105273991B
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
- G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Hematology (AREA)
- Nanotechnology (AREA)
- Urology & Nephrology (AREA)
- Pathology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
Applications Claiming Priority (11)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US12/658591 | 2010-02-08 | ||
| US12/658,591 US9605307B2 (en) | 2010-02-08 | 2010-02-08 | Systems and methods for forming a nanopore in a lipid bilayer |
| US12/658,604 US8324914B2 (en) | 2010-02-08 | 2010-02-08 | Systems and methods for characterizing a molecule |
| US12/658,603 US20110287414A1 (en) | 2010-02-08 | 2010-02-08 | Systems and methods for identifying a portion of a molecule |
| US12/658,602 US20120052188A1 (en) | 2010-02-08 | 2010-02-08 | Systems and methods for assembling a lipid bilayer on a substantially planar solid surface |
| US12/658602 | 2010-02-08 | ||
| US12/658,601 US20110192723A1 (en) | 2010-02-08 | 2010-02-08 | Systems and methods for manipulating a molecule in a nanopore |
| US12/658603 | 2010-02-08 | ||
| US12/658601 | 2010-02-08 | ||
| US12/658604 | 2010-02-08 | ||
| CN201180016647.8A CN103298984B (zh) | 2010-02-08 | 2011-02-04 | 用于在纳米孔中操作分子的系统和方法 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201180016647.8A Division CN103298984B (zh) | 2010-02-08 | 2011-02-04 | 用于在纳米孔中操作分子的系统和方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105273991A CN105273991A (zh) | 2016-01-27 |
| CN105273991B true CN105273991B (zh) | 2019-05-10 |
Family
ID=44355716
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201180016647.8A Active CN103298984B (zh) | 2010-02-08 | 2011-02-04 | 用于在纳米孔中操作分子的系统和方法 |
| CN201510614358.8A Active CN105273991B (zh) | 2010-02-08 | 2011-02-04 | 用于在纳米孔中操作分子的系统和方法 |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201180016647.8A Active CN103298984B (zh) | 2010-02-08 | 2011-02-04 | 用于在纳米孔中操作分子的系统和方法 |
Country Status (7)
| Country | Link |
|---|---|
| EP (2) | EP2534284B1 (enExample) |
| JP (2) | JP5858931B2 (enExample) |
| CN (2) | CN103298984B (enExample) |
| AU (1) | AU2011213234B2 (enExample) |
| CA (2) | CA2995178C (enExample) |
| GB (2) | GB2490847B8 (enExample) |
| WO (1) | WO2011097028A1 (enExample) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11603562B2 (en) | 2018-05-22 | 2023-03-14 | Axbio Inc. | Methods, systems, and compositions for nucleic acid sequencing |
| US12252742B2 (en) | 2021-09-28 | 2025-03-18 | Axbio Inc. | Methods for processing a nucleic acid sample and compositions thereof |
Families Citing this family (72)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101495656B (zh) | 2006-06-07 | 2017-02-08 | 纽约哥伦比亚大学理事会 | 采用带修饰的核苷酸通过纳米通道进行dna序列测定 |
| US8324914B2 (en) | 2010-02-08 | 2012-12-04 | Genia Technologies, Inc. | Systems and methods for characterizing a molecule |
| US9605307B2 (en) | 2010-02-08 | 2017-03-28 | Genia Technologies, Inc. | Systems and methods for forming a nanopore in a lipid bilayer |
| US9678055B2 (en) | 2010-02-08 | 2017-06-13 | Genia Technologies, Inc. | Methods for forming a nanopore in a lipid bilayer |
| WO2012083249A2 (en) | 2010-12-17 | 2012-06-21 | The Trustees Of Columbia University In The City Of New York | Dna sequencing by synthesis using modified nucleotides and nanopore detection |
| GB2500360B (en) | 2010-12-22 | 2019-10-23 | Genia Tech Inc | Nanopore-based single DNA molecule characterization, identification and isolation using speed bumps |
| US9581563B2 (en) | 2011-01-24 | 2017-02-28 | Genia Technologies, Inc. | System for communicating information from an array of sensors |
| US9110478B2 (en) | 2011-01-27 | 2015-08-18 | Genia Technologies, Inc. | Temperature regulation of measurement arrays |
| WO2013067208A1 (en) | 2011-11-02 | 2013-05-10 | Genia Technologies, Inc. | Systems and methods for determining genetic data |
| EP3540077B1 (en) * | 2012-02-16 | 2021-01-27 | Genia Technologies, Inc. | Methods for creating bilayers for use with nanopore sensors |
| US8986629B2 (en) | 2012-02-27 | 2015-03-24 | Genia Technologies, Inc. | Sensor circuit for controlling, detecting, and measuring a molecular complex |
| ES2906186T3 (es) | 2012-04-09 | 2022-04-13 | Univ Columbia | Método para la preparación de nanoporo y usos del mismo |
| EP2861768A4 (en) | 2012-06-15 | 2016-03-02 | Genia Technologies Inc | CHIP SETUP AND HIGH ACCURACY NUCLEIC ACID SEQUENCING |
| WO2013191793A1 (en) | 2012-06-20 | 2013-12-27 | The Trustees Of Columbia University In The City Of New York | Nucleic acid sequencing by nanopore detection of tag molecules |
| US9605309B2 (en) | 2012-11-09 | 2017-03-28 | Genia Technologies, Inc. | Nucleic acid sequencing using tags |
| US20150346188A1 (en) * | 2012-12-21 | 2015-12-03 | Ibis Biosciences, Inc. | Stabilized nanopore and micropore structures and methods for making and using the same |
| US9759711B2 (en) | 2013-02-05 | 2017-09-12 | Genia Technologies, Inc. | Nanopore arrays |
| US10732183B2 (en) | 2013-03-15 | 2020-08-04 | The Trustees Of Columbia University In The City Of New York | Method for detecting multiple predetermined compounds in a sample |
| DE102013206657A1 (de) * | 2013-04-15 | 2014-10-16 | Siemens Aktiengesellschaft | Verfahren zum Gewinnen von mindestens einem doppelsträngigen Nukleinsäure-Fragment |
| US9551697B2 (en) * | 2013-10-17 | 2017-01-24 | Genia Technologies, Inc. | Non-faradaic, capacitively coupled measurement in a nanopore cell array |
| EP3640349A3 (en) | 2013-10-23 | 2020-07-29 | Roche Sequencing Solutions, Inc. | High speed molecular sensing with nanopores |
| US9567630B2 (en) | 2013-10-23 | 2017-02-14 | Genia Technologies, Inc. | Methods for forming lipid bilayers on biochips |
| DE102013223143B4 (de) | 2013-11-13 | 2025-08-21 | Jumatech Gmbh | Leiterplatte mit wenigstens einem eingebetteten Präzisionswiderstand und Verfahren zur Herstellung einer Leiterplatte |
| CN106715453B (zh) | 2014-03-24 | 2021-04-30 | 哥伦比亚大学董事会 | 用于生产带标签的核苷酸的化学方法 |
| US10006899B2 (en) * | 2014-03-25 | 2018-06-26 | Genia Technologies, Inc. | Nanopore-based sequencing chips using stacked wafer technology |
| WO2015175789A1 (en) | 2014-05-14 | 2015-11-19 | Mcruer Robert N | Translocation control for sensing by a nanopore |
| CN110954686B (zh) * | 2014-07-31 | 2021-09-10 | 伊鲁米那股份有限公司 | 混合纳米孔传感器 |
| WO2016069806A2 (en) | 2014-10-31 | 2016-05-06 | Genia Technologies, Inc. | Alpha-hemolysin variants with altered characteristics |
| US10060903B2 (en) * | 2014-11-05 | 2018-08-28 | Genia Technologies, Inc. | Exporting measurements of nanopore arrays |
| US9863904B2 (en) | 2014-12-19 | 2018-01-09 | Genia Technologies, Inc. | Nanopore-based sequencing with varying voltage stimulus |
| US9557294B2 (en) | 2014-12-19 | 2017-01-31 | Genia Technologies, Inc. | Nanopore-based sequencing with varying voltage stimulus |
| JP6538348B2 (ja) * | 2014-12-26 | 2019-07-03 | 株式会社東芝 | 粒子測定装置 |
| WO2016106689A1 (en) * | 2014-12-31 | 2016-07-07 | Coyote Bioscience Co., Ltd. | Detection of nucleic acid molecules using nanopores and tags |
| US10308918B2 (en) | 2015-02-02 | 2019-06-04 | Roche Molecular Systems, Inc. | Polymerase variants |
| CN104713932B (zh) * | 2015-03-24 | 2017-03-29 | 清华大学 | 一种交流模式的多参数纳米孔单分子分析仪 |
| US10526588B2 (en) | 2015-05-14 | 2020-01-07 | Roche Sequencing Solutions, Inc. | Polymerase variants and uses thereof |
| US10174371B2 (en) * | 2015-08-05 | 2019-01-08 | Genia Technologies, Inc. | Use of titanium nitride as an electrode in non-faradaic electrochemical cell |
| WO2017050722A1 (en) | 2015-09-22 | 2017-03-30 | Genia Technologies, Inc. | Ompg variants |
| US10126262B2 (en) | 2015-09-24 | 2018-11-13 | Genia Technologies, Inc. | Differential output of analog memories storing nanopore measurement samples |
| US10935512B2 (en) | 2015-09-24 | 2021-03-02 | Roche Sequencing Solutions, Inc. | Encoding state change of nanopore to reduce data size |
| WO2017060457A1 (en) | 2015-10-07 | 2017-04-13 | Selma Diagnostics Aps | Flow system and methods for digital counting |
| US10371664B2 (en) * | 2016-01-21 | 2019-08-06 | Roche Molecular Systems, Inc. | Use of titanium nitride as a counter electrode |
| US10590480B2 (en) | 2016-02-29 | 2020-03-17 | Roche Sequencing Solutions, Inc. | Polymerase variants |
| ES2880331T3 (es) | 2016-02-29 | 2021-11-24 | Genia Tech Inc | Variantes de polimerasa |
| US10640822B2 (en) | 2016-02-29 | 2020-05-05 | Iridia, Inc. | Systems and methods for writing, reading, and controlling data stored in a polymer |
| US20170268052A1 (en) | 2016-02-29 | 2017-09-21 | Genia Technologies, Inc. | Polymerase-template complexes |
| US10859562B2 (en) | 2016-02-29 | 2020-12-08 | Iridia, Inc. | Methods, compositions, and devices for information storage |
| CN108699539B (zh) | 2016-02-29 | 2022-11-18 | 吉尼亚科技公司 | 外切核酸酶缺陷的聚合酶 |
| US10438662B2 (en) | 2016-02-29 | 2019-10-08 | Iridia, Inc. | Methods, compositions, and devices for information storage |
| US10233486B2 (en) | 2016-03-30 | 2019-03-19 | Roche Sequencing Solutions, Inc. | Method for inline bilayer capacitance monitoring |
| US10465240B2 (en) | 2016-03-30 | 2019-11-05 | Roche Sequencing Solutions, Inc. | Electrical enhancement of bilayer formation |
| US10155979B2 (en) * | 2016-03-30 | 2018-12-18 | Roche Molecular Systems, Inc. | Non-destructive bilayer monitoring using measurement of bilayer response to electrical stimulus |
| US11124827B2 (en) | 2016-06-23 | 2021-09-21 | Roche Sequencing Solutions, Inc. | Period-to-period analysis of AC signals from nanopore sequencing |
| US11035854B2 (en) | 2016-07-29 | 2021-06-15 | Selma Diagnostics Aps | Methods in digital counting |
| WO2018054970A2 (en) | 2016-09-22 | 2018-03-29 | F. Hoffmann-La Roche Ag | Pol6 polymerase variants |
| EP3526603B1 (en) * | 2016-10-12 | 2022-07-20 | F. Hoffmann-La Roche AG | Nanopore voltage methods |
| GB201619930D0 (en) * | 2016-11-24 | 2017-01-11 | Oxford Nanopore Tech | Apparatus and methods for controlling insertion of a membrane channel into a membrane |
| CN110268045B (zh) | 2016-12-15 | 2023-02-21 | 豪夫迈·罗氏有限公司 | 自适应纳米孔信号压缩 |
| EP3646025B1 (en) * | 2017-06-29 | 2021-08-04 | President and Fellows of Harvard College | Deterministic stepping of polymers through a nanopore |
| CN107436317A (zh) * | 2017-07-21 | 2017-12-05 | 河南金泰生物技术股份有限公司 | 一种利用纳米通道技术检测癌症的方法 |
| CN107377020B (zh) * | 2017-08-04 | 2019-09-17 | 深圳市梅丽纳米孔科技有限公司 | 微流控芯片及基于微流控芯片的生物标记物的检测方法 |
| CN107688726B (zh) * | 2017-09-21 | 2021-09-07 | 深圳市易基因科技有限公司 | 基于液相捕获技术判定单基因病相关拷贝数缺失的方法 |
| CN111212919B (zh) * | 2017-09-22 | 2023-12-26 | 豪夫迈·罗氏有限公司 | 纳米孔测序单元中的双电层电容的测量 |
| EP3732484B1 (en) | 2017-12-28 | 2023-06-07 | F. Hoffmann-La Roche AG | Measuring and removing noise in stochastic signals from a nanopore dna sequencing system driven by an alternating signal |
| CA3044782A1 (en) | 2017-12-29 | 2019-06-29 | Clear Labs, Inc. | Automated priming and library loading device |
| CN108300805A (zh) * | 2018-01-15 | 2018-07-20 | 成都齐碳科技有限公司 | 一种确认生命体的方法 |
| JP2021525095A (ja) | 2018-05-28 | 2021-09-24 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | Ｄｎａ−ポア−ポリメラーゼ複合体の酵素的濃縮 |
| CN113260449B (zh) * | 2018-12-11 | 2023-09-29 | 豪夫迈·罗氏有限公司 | 用于膜中自限性蛋白质孔插入的系统和方法 |
| JP2020153996A (ja) * | 2020-05-29 | 2020-09-24 | 株式会社東芝 | 分析チップ |
| US11837302B1 (en) | 2020-08-07 | 2023-12-05 | Iridia, Inc. | Systems and methods for writing and reading data stored in a polymer using nano-channels |
| CN112098489B (zh) * | 2020-08-19 | 2022-11-15 | 孙经纬 | 一种定量dna甲基化程度的电化学方法及系统 |
| CN112300913A (zh) * | 2020-11-11 | 2021-02-02 | 深圳市儒翰基因科技有限公司 | 一种dna测序装置、固态纳米孔阵列及其制备方法 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1712891A2 (en) * | 2005-04-15 | 2006-10-18 | Agilent Technologies, Inc., A Delaware Corporation | Molecular resonant tunneling sensor and methods of fabricating and using the same |
| WO2008124107A1 (en) * | 2007-04-04 | 2008-10-16 | The Regents Of The University Of California | Compositions, devices, systems, and methods for using a nanopore |
| WO2009005547A2 (en) * | 2007-03-09 | 2009-01-08 | University Of Utah Research Foundation | Method for electrostatic trapping of molecules in nanopores |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6916665B2 (en) * | 2000-02-11 | 2005-07-12 | The Texas A&M University System | Biosensor compositions and methods of use |
| US6913697B2 (en) * | 2001-02-14 | 2005-07-05 | Science & Technology Corporation @ Unm | Nanostructured separation and analysis devices for biological membranes |
| CA2497139A1 (en) * | 2002-09-11 | 2004-03-25 | Synamem Corporation | Membrane-based assays |
| JP2006526777A (ja) * | 2003-02-28 | 2006-11-24 | ブラウン ユニバーシティー | ナノ細孔、その使用方法、その作成方法、及びそれを使用して生体分子を特徴付ける方法 |
| US7112433B2 (en) * | 2003-04-24 | 2006-09-26 | Hewlett-Packard Development Company, L.P. | Electrical analysis of biological membranes |
| US7279337B2 (en) * | 2004-03-10 | 2007-10-09 | Agilent Technologies, Inc. | Method and apparatus for sequencing polymers through tunneling conductance variation detection |
| WO2006012571A1 (en) * | 2004-07-23 | 2006-02-02 | Electronic Bio Sciences, Llc | Method and apparatus for sensing a time varying current passing through an ion channel |
| AU2005272823B2 (en) * | 2004-08-13 | 2012-04-12 | President And Fellows Of Harvard College | An ultra high-throughput opti-nanopore DNA readout platform |
| US20060073489A1 (en) * | 2004-10-05 | 2006-04-06 | Gangqiang Li | Nanopore separation devices and methods of using same |
| EP1842061A4 (en) * | 2004-12-21 | 2009-05-13 | Texas A & M Univ Sys | HIGH-TEMPERATURE ION CHANNELS AND ION PORES |
| TWI287041B (en) * | 2005-04-27 | 2007-09-21 | Jung-Tang Huang | An ultra-rapid DNA sequencing method with nano-transistors array based devices |
| US7849581B2 (en) * | 2006-05-05 | 2010-12-14 | University Of Utah Research Foundation | Nanopore electrode, nanopore membrane, methods of preparation and surface modification, and use thereof |
| US7777505B2 (en) * | 2006-05-05 | 2010-08-17 | University Of Utah Research Foundation | Nanopore platforms for ion channel recordings and single molecule detection and analysis |
| GB0625070D0 (en) * | 2006-12-15 | 2007-01-24 | Imp Innovations Ltd | Characterization of molecules |
| AU2008217579A1 (en) * | 2007-02-20 | 2008-08-28 | Oxford Nanopore Technologies Limited | Formation of lipid bilayers |
| WO2009045472A1 (en) * | 2007-10-02 | 2009-04-09 | President And Fellows Of Harvard College | Capture, recapture, and trapping of molecules with a nanopore |
| GB0724736D0 (en) * | 2007-12-19 | 2008-01-30 | Oxford Nanolabs Ltd | Formation of layers of amphiphilic molecules |
| US20090215050A1 (en) * | 2008-02-22 | 2009-08-27 | Robert Delmar Jenison | Systems and methods for point-of-care amplification and detection of polynucleotides |
| GB0808856D0 (en) * | 2008-05-15 | 2008-06-25 | Univ Warwick | Fabricated nanopores and micropores for chemical and biochemical analysis |
| CN102741430B (zh) * | 2009-12-01 | 2016-07-13 | 牛津楠路珀尔科技有限公司 | 生化分析仪器、用于进行生化分析的第一模块以及相关方法 |
-
2011
- 2011-02-04 CA CA2995178A patent/CA2995178C/en active Active
- 2011-02-04 EP EP11740145.5A patent/EP2534284B1/en active Active
- 2011-02-04 EP EP14191378.0A patent/EP2843405B8/en active Active
- 2011-02-04 CA CA2788331A patent/CA2788331C/en active Active
- 2011-02-04 AU AU2011213234A patent/AU2011213234B2/en active Active
- 2011-02-04 WO PCT/US2011/000205 patent/WO2011097028A1/en not_active Ceased
- 2011-02-04 CN CN201180016647.8A patent/CN103298984B/zh active Active
- 2011-02-04 GB GB201216026A patent/GB2490847B8/en active Active
- 2011-02-04 GB GB1216656.7A patent/GB2496019B/en active Active
- 2011-02-04 JP JP2012551978A patent/JP5858931B2/ja active Active
- 2011-02-04 CN CN201510614358.8A patent/CN105273991B/zh active Active
-
2015
- 2015-12-15 JP JP2015244065A patent/JP6124982B2/ja active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1712891A2 (en) * | 2005-04-15 | 2006-10-18 | Agilent Technologies, Inc., A Delaware Corporation | Molecular resonant tunneling sensor and methods of fabricating and using the same |
| WO2009005547A2 (en) * | 2007-03-09 | 2009-01-08 | University Of Utah Research Foundation | Method for electrostatic trapping of molecules in nanopores |
| WO2008124107A1 (en) * | 2007-04-04 | 2008-10-16 | The Regents Of The University Of California | Compositions, devices, systems, and methods for using a nanopore |
Non-Patent Citations (1)
| Title |
|---|
| Self-assembled a-hemolysin pores in an S-layer-supported;Bernhard Schuster等;《Biochimica et Biophysica Acta》;19981231(第1370期);第280-288页 |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11603562B2 (en) | 2018-05-22 | 2023-03-14 | Axbio Inc. | Methods, systems, and compositions for nucleic acid sequencing |
| US12227801B2 (en) | 2018-05-22 | 2025-02-18 | Axbio Inc. | Methods, systems, and compositions for nucelic acid sequencing |
| US12252742B2 (en) | 2021-09-28 | 2025-03-18 | Axbio Inc. | Methods for processing a nucleic acid sample and compositions thereof |
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