CN105265755A - Special enzyme preparation containing probiotics for suckling piglets and preparation method thereof - Google Patents
Special enzyme preparation containing probiotics for suckling piglets and preparation method thereof Download PDFInfo
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- CN105265755A CN105265755A CN201410337194.4A CN201410337194A CN105265755A CN 105265755 A CN105265755 A CN 105265755A CN 201410337194 A CN201410337194 A CN 201410337194A CN 105265755 A CN105265755 A CN 105265755A
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Abstract
The present invention discloses a special enzyme preparation containing probiotics for suckling piglets. The special enzyme preparation comprises the following components in parts by weight: 30-50 parts of xylanase, 7-15 parts of cellulose, 5-10 parts of mannanase, 20-30 parts of beta-glucanase, 20-35 parts of protease, 20-30 parts of amylase, 10-20 parts of alpha-galactosidase, 5-15 parts of bacillus licheniformis culture, 15-28 parts of high temperature-resistant lactic acid bacteria, and 110-130 parts of yeast cell wall polysaccharides. The present invention also discloses a preparation method of the special enzyme preparation containing probiotics for suckling piglets. Since the ingredients are reasonably matched and the compound enzyme and probiotics have synergistic effects, the special enzyme preparation containing probiotics for suckling piglets can improve the production performance of suckling piglets to a greater degree compared with the commercially available enzyme preparation with the same addition cost.
Description
Technical field
The invention belongs to enzymic preparation field, be specifically related to a kind of newborn piglet special enzyme preparation and preparation method thereof.
Background technology
The physiological development of breast piglet is unsound, the digestive ferment of self secretion is obviously not enough, especially after wean, food transfers solid feed to by breast milk, there is larger emergency reaction in breast piglet, the digestive ferment of himself secretion cannot meet digesting and assimilating chyme Middle nutrition material, causes feed waste, affects its growth performance.And ubiquity SNSP ANFs in feed, chymeviscosity can be made to increase, and minimizing digestive ferment contacts with chyme Middle nutrition element, reduces utilization rate.Chyme blocks and is beneficial to fermentable in alimentary canal, breaks archenteric flora balance.In addition, the large molecule such as SNSP has powerful absorbent function, can cause human body serious water shortage, show as grice diarrhoea, pathogenic bacteria in enteron aisle amount reproduction, germinate disease, simultaneously the large wriggling difficulty of chymeviscosity causes digestive organs hyperplasia, affect human body health.
Company of existing foreign country obtains certain effect by adopting complex enzyme formulation technology specific aim to solve the problem, namely the complex enzyme formulation that scientific compatibility is made up of zytase, cellulase, 1,4 beta-glucanase, protease, amylase is rationally added, improve efficiency of feed utilization to a certain extent, and had certain change in newborn grice diarrhoea rate, skin hair color, death rate.But at present enzyme preparation considers that more is the theory action of enzyme, namely the effect that enzyme plays in Utopian environment, and the digestive tract environment of animal is a comparatively complicated system, the less consideration of prior art and the movable impact on enzyme effect system of research digestive tract environment flora, add that domestic Diet Formula difference is large, different daily ration ANFs there are differences, and the specific aim of simple enzyme preparation can be short of to some extent, and it is very limited that culture efficiency improves space.
Because existing enzyme preparation exists above-mentioned shortcoming, its application as newborn piglet special enzyme preparation and popularization certainly will be affected, therefore, develop the focus that Novel breast piglet special enzyme preparation has become current Animal husbandry production and scientific and technical personnel's concern.
Summary of the invention
In view of this, the object of the present invention is to provide a kind of newborn piglet special enzyme preparation containing probio and preparation method thereof, fully take into account self secretase deficiency and archenteric flora requirement of balance before and after newborn weaned piglet, improve efficiency of feed utilization, improve culture efficiency.
To achieve these goals, the invention provides following technical scheme: a kind of newborn piglet special enzyme preparation containing probio, is made up of the component of following parts by weight:
Zytase 30-50 part, cellulase 7-15 part, mannase 5-10 part, 1,4 beta-glucanase 20-30 part, protease 20-35 part, amylase 2 0-30 part, alpha-galactosidase 10-20 part, bacillus licheniformis culture 5-15 part, high temperature resistant lactic acid bacteria 15-28 part, yeast cell wall polysaccharide 110-130 part.
Preferably, described a kind of newborn piglet special enzyme preparation containing probio, is made up of the component of following parts by weight:
Zytase 40 parts, cellulase 10 parts, mannase 8 parts, 1,4 beta-glucanase part 25 parts, 26 parts, protease, amylase 25 parts, alpha-galactosidase 15 parts, bacillus licheniformis culture 10 parts, high temperature resistant lactic acid bacteria 20 parts, yeast cell wall polysaccharide 120 parts.
As mentioned above containing a preparation method for the newborn piglet special enzyme preparation of probio, comprise the steps:
(1) preparation of bacillus licheniformis culture
A seed culture
Bacillus licheniformis is cultivated through slant strains shaking flask and first class seed pot, cultivation temperature 35-45 DEG C, pH4.5-7.0.
The culture medium of described seed tank culture is composed of the following components: peptone 1%, dusty yeast 0.5%, soluble starch 1%, NaCl1%; In 121-123 DEG C of sterilizing 30-40min.
B ferment tank
First class seed pot zymotic fluid is contained in the fermentation tank of fermentation medium with 3% inoculum concentration access, throughput (V/V) 1: 2.5, mixing speed 200-300r/min, cultivation temperature 35-45 DEG C, constant temperature culture time 10-16h, dissolved oxygen 16-32%.
Described fermentation medium is composed of the following components: wheat bran 76g, corn flour 55g, beancake powder 21g, corn steep liquor 4g, brown shorts 26g, dusty yeast 8g, trehalose 35g, potassium dihydrogen phosphate 2g, dipotassium hydrogen phosphate 1.5g, ammonium sulfate 2g, natrium citricum 3g, defoamer 0.5g, pure water 1000ml.
The preparation method of described fermentation medium is as follows: take raw material in proportion, and first by corn flour, beancake powder and water drop in material-compound tank in proportion, adjust pH to be 4.0, add middle temperature amylase according to the ratio of 5u/g corn flour, stir and be warming up to 65 DEG C, insulation 30min, add alpha-amylase according to the ratio of 30u/g corn flour again, continue stirring and be warming up to 90 DEG C, insulation 30min, then other raw materials in nutrient media components are added in proportion, adjust pH to be 5.5, be warming up to 123 DEG C, insulated sterilizing 30-40min.
C fermentation liquor coarse filtration, ultrafiltration postlyophilization, ultramicro grinding obtains bacillus licheniformis culture.
(2) mixing of each component
Take zytase 30-50 part, cellulase 7-15 part, mannase 5-10 part, 1,4 beta-glucanase 20-30 part, protease 20-35 part, amylase 2 0-30 part, alpha-galactosidase 10-20 part, bacillus licheniformis culture 5-15 part, high temperature resistant lactic acid bacteria 15-28 part, yeast cell wall polysaccharide 110-130 part, high temperature resistant lactic acid bacteria and bacillus licheniformis are mixed with yeast cell wall polysaccharide in proportion, then mixes with all the other compositions.
The present invention meets People's Republic of China (PRC) agricultural industry criteria NY/T722-2003.The probio that the present invention adds is high temperature resistant lactic acid bacteria, bacillus licheniformis culture, and the interpolation of probio can adjust gut flora, suppresses the growth of the harmful bacterias such as Escherichia coli, salmonella, comma bacillus, promotes intestinal health.It is not enough that combination enzyme plays self secretion digestive ferment after supplementary newborn weaned piglet, reduces the effect of the ANFs side reactions such as SNSP in feed, improve efficiency of feed utilization.
Reasonable mixture ratio of the present invention, not only to consider before and after newborn weaned piglet that self secretase is not enough, archenteric flora requirement of balance and fully digest and assimilate principle to feed, effectively supplements the deficiency of endogenous enzymes, and adds probio and carry out balanced body environment.Compared to commercially available combination enzyme preparation, under identical interpolation cost, the present invention, to newborn piglet daily gain, feedstuff-meat ratio, diarrhea rate shows more excellent, illustrates that the present invention can promote the production performance of newborn piglet greatly.
Detailed description of the invention
Describe the present invention below in conjunction with embodiment, the description of this part is only exemplary and explanatory, should to protection scope of the present invention by any restriction.
In the present invention's formula: zytase, cellulase, mannonase 1,4 beta-glucanase, protease, amylase, alpha-galactosidase, yeast cell wall polysaccharide all adopt Hongyingxiang Biological Engineering Co., Ltd., Hunan's product; High temperature resistant lactic acid bacteria is Ya Xin bio tech ltd, Taiwan product product.
Embodiment 1: a kind of newborn piglet special enzyme preparation containing probio
Fill a prescription as follows:
Zytase 30 parts, cellulase 7 parts, mannase 5 parts, 1,4 beta-glucanase part 20 parts, 20 parts, protease, amylase 20 part, alpha-galactosidase 10 parts, bacillus licheniformis culture 5 parts, high temperature resistant lactic acid bacteria 15 parts, yeast cell wall polysaccharide 110 parts, high temperature resistant lactic acid bacteria 15 parts, bacillus licheniformis culture 5 parts, yeast cell wall polysaccharide 110 parts.
Preparation method is as follows:
(1) preparation of bacillus licheniformis culture:
A seed culture
Bacillus licheniformis is cultivated through slant strains shaking flask and first class seed pot, cultivation temperature 35-45 DEG C, pH4.5-7.0.
The culture medium of described seed tank culture is composed of the following components: peptone 1%, dusty yeast 0.5%, soluble starch 1%, NaCl1%; In 121-123 DEG C of sterilizing 30-40min.
B ferment tank
First class seed pot zymotic fluid is contained in the fermentation tank of fermentation medium with 3% inoculum concentration access, throughput (V/V) 1: 2.5, mixing speed 200-300r/min, cultivation temperature 35-45 DEG C, constant temperature culture time 10-16h, dissolved oxygen 16-32%.
Described fermentation medium is composed of the following components: wheat bran 76g, corn flour 55g, beancake powder 21g, corn steep liquor 4g, brown shorts 26g, dusty yeast 8g, trehalose 35g, potassium dihydrogen phosphate 2g, dipotassium hydrogen phosphate 1.5g, ammonium sulfate 2g, natrium citricum 3g, defoamer 0.5g, pure water 1000ml.
The preparation method of described fermentation medium is as follows: take raw material in proportion, and first by corn flour, beancake powder and water drop in material-compound tank in proportion, adjust pH to be 4.0, add middle temperature amylase according to the ratio of 5u/g corn flour, stir and be warming up to 65 DEG C, insulation 30min, add alpha-amylase according to the ratio of 30u/g corn flour again, continue stirring and be warming up to 90 DEG C, insulation 30min, then other raw materials in nutrient media components are added in proportion, adjust pH to be 5.5, be warming up to 123 DEG C, insulated sterilizing 30-40min.
C fermentation liquor coarse filtration, ultrafiltration postlyophilization, ultramicro grinding obtains bacillus licheniformis culture.
(2) take each composition in proportion, high temperature resistant lactic acid bacteria and bacillus licheniformis are mixed with yeast cell wall polysaccharide in proportion, then mixes with all the other compositions.
Embodiment 2: a kind of newborn piglet special enzyme preparation containing probio
Fill a prescription as follows:
Zytase 40 parts, cellulase 10 parts, mannase 8 parts, 1,4 beta-glucanase part 25 parts, 26 parts, protease, amylase 25 parts, alpha-galactosidase 15 parts, high temperature resistant lactic acid bacteria 20 parts, bacillus licheniformis culture 10 parts, yeast cell wall polysaccharide 120 parts.
Preparation method is as follows:
(1) preparation of bacillus licheniformis culture:
A seed culture
Bacillus licheniformis is cultivated through slant strains shaking flask and first class seed pot, cultivation temperature 35-45 DEG C, pH4.5-7.0.
The culture medium of described seed tank culture is composed of the following components: peptone 1%, dusty yeast 0.5%, soluble starch 1%, NaCl1%; In 121-123 DEG C of sterilizing 30-40min.
B ferment tank
First class seed pot zymotic fluid is contained in the fermentation tank of fermentation medium with 3% inoculum concentration access, throughput (V/V) 1: 2.5, mixing speed 200-300r/min, cultivation temperature 35-45 DEG C, constant temperature culture time 10-16h, dissolved oxygen 16-32%.
Described fermentation medium is composed of the following components: wheat bran 76g, corn flour 55g, beancake powder 21g, corn steep liquor 4g, brown shorts 26g, dusty yeast 8g, trehalose 35g, potassium dihydrogen phosphate 2g, dipotassium hydrogen phosphate 1.5g, ammonium sulfate 2g, natrium citricum 3g, defoamer 0.5g, pure water 1000ml.
The preparation method of described fermentation medium is as follows: take raw material in proportion, first by corn flour, beancake powder and water drop in material-compound tank in proportion, pH is adjusted to be 4.0, middle temperature amylase is added according to the ratio of 5u/g corn flour, stirring is warming up to 65 DEG C, insulation 30min, then adds alpha-amylase according to the ratio of 30u/g corn flour, continue stirring and be warming up to 90 DEG C, insulation 30min, then adds other raw materials in nutrient media components in proportion, adjusts pH to be 5.5, be warming up to 123 DEG C, insulated sterilizing 30-40min.
C fermentation liquor coarse filtration, ultrafiltration postlyophilization, ultramicro grinding obtains bacillus licheniformis culture.
(2) take each composition in proportion, high temperature resistant lactic acid bacteria and bacillus licheniformis are mixed with yeast cell wall polysaccharide in proportion, then mixes with all the other compositions.
Embodiment 3: a kind of newborn piglet special enzyme preparation containing probio
Fill a prescription as follows:
Zytase 50 parts, cellulase 15 parts, mannase 10 parts, 1,4 beta-glucanase part 30 parts, protease 35 parts, amylase 30 parts, alpha-galactosidase 20 parts, high temperature resistant lactic acid bacteria 28 parts, bacillus licheniformis culture 15 parts, yeast cell wall polysaccharide 130 parts.
Preparation method is as follows:
(1) preparation of bacillus licheniformis culture:
A seed culture
Bacillus licheniformis is cultivated through slant strains shaking flask and first class seed pot, cultivation temperature 35-45 DEG C, pH4.5-7.0.
The culture medium of described seed tank culture is composed of the following components: peptone 1%, dusty yeast 0.5%, soluble starch 1%, NaCl1%; In 121-123 DEG C of sterilizing 30-40min.
B ferment tank
First class seed pot zymotic fluid is contained in the fermentation tank of fermentation medium with 3% inoculum concentration access, throughput (V/V) 1: 2.5, mixing speed 200-300r/min, cultivation temperature 35-45 DEG C, constant temperature culture time 10-16h, dissolved oxygen 16-32%.
Described fermentation medium is composed of the following components: wheat bran 76g, corn flour 55g, beancake powder 21g, corn steep liquor 4g, brown shorts 26g, dusty yeast 8g, trehalose 35g, potassium dihydrogen phosphate 2g, dipotassium hydrogen phosphate 1.5g, ammonium sulfate 2g, natrium citricum 3g, defoamer 0.5g, pure water 1000ml.
The preparation method of described fermentation medium is as follows: take raw material in proportion, and first by corn flour, beancake powder and water drop in material-compound tank in proportion, adjust pH to be 4.0, add middle temperature amylase according to the ratio of 5u/g corn flour, stir and be warming up to 65 DEG C, insulation 30min, add alpha-amylase according to the ratio of 30u/g corn flour again, continue stirring and be warming up to 90 DEG C, insulation 30min, then other raw materials in nutrient media components are added in proportion, adjust pH to be 5.5, be warming up to 123 DEG C, insulated sterilizing 30-40min.
C fermentation liquor coarse filtration, ultrafiltration postlyophilization, ultramicro grinding obtains bacillus licheniformis culture.
(2) take each composition in proportion, high temperature resistant lactic acid bacteria and bacillus licheniformis are mixed with yeast cell wall polysaccharide in proportion, then mixes with all the other compositions.
Comparative experimental example
Basically identical, healthy, the well-grown 28 age in days DLY weanling pig 150 of body weight is selected in this test.Weanling pig is divided into 5 groups at random, namely control group, enzyme-added group, embodiment 1 group, embodiment 2 groups, embodiment 3 groups, often organize 3 repetitions, each repetition 10.Embodiment 1 group, embodiment 2 groups, embodiment 3 groups are fed the daily ration of newborn piglet special enzyme preparation containing probio of interpolation 0.1% above-described embodiment 1, embodiment 2, embodiment 3 respectively.
1 materials and methods
1.1 test material
Test be divided into 5 groups, namely control group, enzyme-added group, embodiment 1 group, embodiment 2 groups, embodiment 3 groups.Control group does not add enzyme preparation, enzyme-added group for add 0.05% commercially available combination enzyme preparation in daily ration, embodiment group is in daily ration, add 0.1% the present invention containing the newborn piglet special enzyme preparation of probio." nutritional need of pig " recommended according to NRC (1998) and in recent years to weanling pig feeding level preparation daily ration, formula in table 1.
| Product | Unit price |
| Commercially available combination enzyme preparation | 50000 yuan/ton |
| Product of the present invention | 2.4 ten thousand yuan/ton |
1.2 feeding and management
Test is carried out on east wind pig farm, Lei Feng town, Wangcheng County of Hunan Province, and experimental period is 30 days, conventional raising free choice feeding.Require routinely in experiment periods to carry out vaccine injection, castration, expelling parasite.
1.3 Testing index
1.3.1 production performance
Weighing with 7 empty stomaches in 30 day morning for 0 day in test, calculates feed intake, daily gain, daily ingestion amount, feedstuff-meat ratio.
1.3.2 diarrhea rate
Observe every day and record newborn grice diarrhoea situation.Scoring (Barnett is divided with 1-5 according to ight soil situation; 1989), concrete grammar is: 1 is divided into firmly just; 2 are divided into normal homogeneous viscous ight soil; 3 be divided into softer, but the ight soil that part is shaping (mild diarrhea); 4 are divided into half aqueous, starchiness ight soil (mild diarrhea); 5 are divided into watery stools (severe diarrhea).
Head number/test pig of suffering from diarrhoea diarrhea rate (%)=every day total head number X100%.
2 results
As shown in table 2 data, enzyme-added group is significantly higher than control group with the average daily gain of embodiment group and feedstuff-meat ratio, illustrates that the interpolation of combination enzyme can promote the daily gain of newborn piglet, improves food conversion ratio.But because of the synergy with probio, embodiment group is at daily gain, feedstuff-meat ratio, and the every data on diarrhea rate are all better than control group and enzyme-added group.
As shown in table 3 data, enzyme-added group all can significantly be reduced diarrhea of weaned piglets rate with embodiment group, illustrates that combination enzyme alleviates the effect of ANFs in daily ration, facilitates the utilization of newborn piglet to nutriment, reduce daily ration and ferment in large intestine.But embodiment group shows more excellent, illustrate that the reasonable interpolation of probio can promote intestinal health, reduce morbidity rate.
3 brief summaries
Under identical interpolation cost, due to product component reasonable mixture ratio of the present invention, and the synergy of complex enzyme and probio, the production performance of newborn piglet can be promoted greatly.
Table 1 is fed Diet Formula and trophic level
The impact on Production Performance of Weaning Pigs tested by table 2
| Group | Initial weight (KG) | End heavy (KG) | Average daily gain (G) | Average daily feed intake (G) | Feedstuff-meat ratio |
| Control group | 9.34±0.64 | 15.56±1.08 | 203.65±57.02 a | 430.31 | 2.11 |
| Enzyme-added group | 9.11±0.89 | 16.01±1.33 | 225.63±34.05 b | 439.47 | 1.95 |
| Embodiment 1 group | 9.02±1.19 | 16.23±1.34 | 233.11±35.17 c | 445.65 | 1.91 |
| Embodiment 2 groups | 9.13±0.82 | 16.31±1.29 | 234.47±32.86 d | 445.49 | 1.90 |
| Embodiment 3 groups | 9.18±1.19 | 16.29±1.32 | 232.64±34.29 e | 446.67 | 1.92 |
Note: numeric representation is average ± poor, the different lowercase alphabet of shoulder note shows significant difference (P≤0.05).
The comparison of each group of grice diarrhoea rate tested by table 3
| Group | Control group | Enzyme-added group | Embodiment 1 group | Embodiment 2 groups | Embodiment 3 groups |
| Diarrhea rate | 24％ | 8％ | 5％ | 5％ | 6％ |
Claims (4)
1., containing a newborn piglet special enzyme preparation for probio, it is characterized in that, consist of the following composition:
Zytase, cellulase, mannonase 1,4 beta-glucanase, protease, amylase, alpha-galactosidase, bacillus licheniformis culture, high temperature resistant lactic acid bacteria, yeast cell wall polysaccharide.
2. a kind of newborn piglet special enzyme preparation containing probio according to claim 1, is characterized in that, be made up of the component of following parts by weight:
Zytase 30-50 part, cellulase 7-15 part, mannase 5-10 part, 1,4 beta-glucanase 20-30 part, protease 20-35 part, amylase 2 0-30 part, alpha-galactosidase 10-20 part, bacillus licheniformis culture 5-15 part, high temperature resistant lactic acid bacteria 15-28 part, yeast cell wall polysaccharide 110-130 part.
3. a kind of newborn piglet special enzyme preparation containing probio according to claim 2, is characterized in that, be made up of the component of following parts by weight:
Zytase 40 parts, cellulase 10 parts, mannase 8 parts, 1,4 beta-glucanase part 25 parts, 26 parts, protease, amylase 25 parts, alpha-galactosidase 15 parts, bacillus licheniformis culture 10 parts, high temperature resistant lactic acid bacteria 20 parts, yeast cell wall polysaccharide 120 parts.
4., according to the preparation method of the arbitrary described a kind of newborn piglet special enzyme preparation containing probio of claims 1 to 3, it is characterized in that, comprise the steps:
(1) preparation of bacillus licheniformis culture
A seed culture
Bacillus licheniformis is cultivated through slant strains shaking flask and first class seed pot, cultivation temperature 35-45 DEG C, pH4.5-7.0;
The culture medium of described seed tank culture is composed of the following components: peptone 1%, dusty yeast 0.5%, soluble starch 1%, NaCl1%; In 121-123 DEG C of sterilizing 30-40min;
B ferment tank
First class seed pot zymotic fluid is contained in the fermentation tank of fermentation medium with 3% inoculum concentration access, throughput (V/V) 1: 2.5, mixing speed 200-300r/min, cultivation temperature 35-45 DEG C, constant temperature culture time 10-16h, dissolved oxygen 16-32%;
Described fermentation medium is composed of the following components: wheat bran 76g, corn flour 55g, beancake powder 21g, corn steep liquor 4g, brown shorts 26g, dusty yeast 8g, trehalose 35g, potassium dihydrogen phosphate 2g, dipotassium hydrogen phosphate 1.5g, ammonium sulfate 2g, natrium citricum 3g, defoamer 0.5g, pure water 1000ml;
The preparation method of described fermentation medium is as follows: take raw material in proportion, and first by corn flour, beancake powder and water drop in material-compound tank in proportion, adjust pH to be 4.0, add middle temperature amylase according to the ratio of 5u/g corn flour, stir and be warming up to 65 DEG C, insulation 30min, add alpha-amylase according to the ratio of 30u/g corn flour again, continue stirring and be warming up to 90 DEG C, insulation 30min, then other raw materials in nutrient media components are added in proportion, adjust pH to be 5.5, be warming up to 123 DEG C, insulated sterilizing 30-40min;
C fermentation liquor coarse filtration, ultrafiltration postlyophilization, ultramicro grinding obtains bacillus licheniformis culture;
(2) mixing of each component
Take zytase 30-50 part, cellulase 7-15 part, mannase 5-10 part, 1,4 beta-glucanase 20-30 part, protease 20-35 part, amylase 2 0-30 part, alpha-galactosidase 10-20 part, bacillus licheniformis culture 5-15 part, high temperature resistant lactic acid bacteria 15-28 part, yeast cell wall polysaccharide 110-130 part, high temperature resistant lactic acid bacteria and bacillus licheniformis are mixed with yeast cell wall polysaccharide in proportion, then mixes with all the other compositions.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102960580A (en) * | 2012-12-19 | 2013-03-13 | 云南邦格农业集团有限公司 | Compound pre-mixing feed of 4% green fattening pig |
| CN103875935A (en) * | 2013-12-27 | 2014-06-25 | 湖南鸿鹰生物科技有限公司 | Probiotics-containing special complex enzyme for piglets and preparation method of complex enzyme |
| CN103892135A (en) * | 2014-04-15 | 2014-07-02 | 湖南农业大学 | Piglet feed complex enzyme preparation and production method and application thereof |
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- 2014-07-12 CN CN201410337194.4A patent/CN105265755A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102960580A (en) * | 2012-12-19 | 2013-03-13 | 云南邦格农业集团有限公司 | Compound pre-mixing feed of 4% green fattening pig |
| CN103875935A (en) * | 2013-12-27 | 2014-06-25 | 湖南鸿鹰生物科技有限公司 | Probiotics-containing special complex enzyme for piglets and preparation method of complex enzyme |
| CN103892135A (en) * | 2014-04-15 | 2014-07-02 | 湖南农业大学 | Piglet feed complex enzyme preparation and production method and application thereof |
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Application publication date: 20160127 |