CN105265753A - Application of functional oligosaccharide in regulating female animal uterus development performance - Google Patents
Application of functional oligosaccharide in regulating female animal uterus development performance Download PDFInfo
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- CN105265753A CN105265753A CN201410344766.1A CN201410344766A CN105265753A CN 105265753 A CN105265753 A CN 105265753A CN 201410344766 A CN201410344766 A CN 201410344766A CN 105265753 A CN105265753 A CN 105265753A
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Abstract
The present invention discloses an application of a functional oligosaccharide in regulating female animal uterus development performance. Every 1 kg of feed, food or medicines comprises the functional oligosaccharide: 10-1000 mg of oligochitosan. The matches of the functional oligosaccharides, and animal feed, food or medicines can improve the develop performance of female animal uterus, promote uterine blood vessel and muscle development performances of female animals in the periods of weaning, sexual maturity, and pregnancy. The functional oligosaccharides in animal feed, food or medicines are less in addition, free of toxic or side effects, residue, and pollution, good in repeatability, and have no adverse effects on the environment.
Description
Technical field
The present invention relates to a kind of functional oligosaccharide to apply in adjustment female uterus growth performance, specifically, be a kind of functional oligosaccharide being added on active ingredient in animal feed, food or medicine, belong to agro-ecology product technique field.
Background technology
Since reform and opening-up, the animal husbandry growth rate of China reaches 9%, and the animal husbandry output value account for 1/3rd of the general production value of agriculture.But China's animal husbandry development is not powerful.Reproductive performance is not high is one of principal element of restriction China aquaculture fast development.Wherein, gestational period animal miscarriage is one of principal element reducing animal population reproductive performance.Some Animals is few at pregnancy duration uterine blood flow, endometrium blood is for few, cause endometrium receptivity low, be unfavorable for implantation and the growth of body early embryo, and animal clinical gestation efficiency is not high, cause having about 10% ~ 15% out of heat or infertile breeding stock to be eliminated, this can reduce the economic benefit of pig farm, cattle farm and Yang Chang.At present, improve the blood supply of female uterus clinically mainly through injection estrogen, aspirin, vitamin E and Chinese medicine etc., increase vessel density, increase endometrium thickness, and then improve developing womb performance to improve pregnancy rate, but effect differs.Wherein, injection estrogen has damaging effect to animal health, and disturbance endocrine balances, and induced tumor occurs, and the risk increase etc. of VTE occurs, and limits it and promotes the use of.Therefore, need to find one to avoid applying heavy dose of estrogen, still can improve the method that endometrium thickness etc. grows performance.Natural active matter is adopted to regulate the growth performance of female uterus to be a feasible approach, by adding natural active matter (as oligosaccharides) to improve female disposition phase pregnancy rate and development of fetus ability in jenny daily ration, for the reproductive performance improving animal population carries out basis.
By the raw material such as shrimp and crab shells, inulin degrade in vitro generate functional oligosaccharide (as chitosan oligosaccharide, FOS) be widely used in feed industry, these functional sugar content be added in feed are atomic, but the physiological action that they play in animal body is very large, there is regulating intestinal canal health, promote beneficial bacterium growth, suppress the physiological function of harmful intestinal tract bacteria growing.Simultaneously it has the multinomial physiological functions such as anti-oxidant, reducing blood lipid, immunity moderation, penetration enhancement, improves the general level of the health of animal from aggregate level.In recent years, functional oligosaccharide becomes the focus in Animal nutrition research, has played good effect in feed industry.But still do not have functional oligosaccharide to grow the research report of performance impact to female uterus both at home and abroad.
Summary of the invention
It is atomic that the object of the invention is to provide a kind of content, and female uterus can be regulated to grow the functional oligosaccharide of performance.
For achieving the above object, the technical solution used in the present invention is:
Using function oligosaccharides of the present invention is chitosan oligosaccharide, and the chitosan oligosaccharide added in every 1kg feed, food or medicine is 10 ~ 1000mg.
This functional oligosaccharide needs with perfect compound feed, food or medicine used in combination before use.Premix (as 10% concentration) can be fitted in conventional feed addictive, add in perfect compound feed for animal edible.
This functional oligosaccharide feed can regulate the growth performance of jenny weaning period, sexual maturing period and Uterus during Pregnancy well, especially show and improve female uterus inner film thickness and uterus muscle thickness, improve in the important indicator such as uterine vascular number and red blood cell number.
The present invention need weaning period, sexual maturing period, the gestational period Long-term Feeding animal, and Long-term Feeding is to reach optimum efficiency.
Advantage of the present invention has: can improve female uterus inner film thickness, improve uterine vascular number and red blood cell number, addition is few, and the physiologically active of performance is high, continue use at weaning period, sexual maturing period and pregnancy duration and can not produce pollution to environment, be applicable to large-scale application.
Accompanying drawing explanation
Fig. 1. chitosan oligosaccharide on impact (HE dyeing) note: the a-b of Mouse Uterus tissue cross Area morphology in sexual maturing period, control group uterus; C-d, chitosan oligosaccharide group uterus.
Fig. 2. chitosan oligosaccharide organizes the morphologic impact of vertical section (HE dyeing) note: a-c to Mouse Uterus in sexual maturing period, control group uterus; D-f, chitosan oligosaccharide group uterus; BV:bloodvessels, blood vessel; EM:endometrium, endometrium.
Fig. 3. chitosan oligosaccharide on gestation the 6th day Mouse Uterus tissue cross Area morphology impact (HE dyeing) note: a-c, control group uterus; D-f, chitosan oligosaccharide group uterus; BV:bloodvessels, blood vessel.
Fig. 4. chitosan oligosaccharide organizes the morphologic impact of vertical section (HE dyeing) note: a-c, control group uterus to gestation the 6th day Mouse Uterus; D-f, chitosan oligosaccharide group uterus; BV:bloodvessels, blood vessel; RBC:redbloodcells, red blood cell.
Fig. 5. chitosan oligosaccharide affects (HE dyeing) note: a-d, control group uterus to gestation the 19th day Mouse Uterus tissue cross Area morphology; E-h, chitosan oligosaccharide group uterus .BV: blood vessel; RBC: red blood cell; EM: endometrium; LM: diagonal flesh; LOM: longitudinal muscle.
Fig. 6. chitosan oligosaccharide on impact (HE dyeing) note: the a-c of weaning period Mouse Uterus tissue cross Area morphology, control group uterus; D-f, chitosan oligosaccharide group uterus; EM:endometrium, endometrium; BV:bloodvessels, blood vessel.
Fig. 7. chitosan oligosaccharide organizes the morphologic impact of vertical section (HE dyeing) note: a-c to weaning period Mouse Uterus, control group uterus; D-f, chitosan oligosaccharide group uterus; EM:endometrium, endometrium; BV:bloodvessels, blood vessel.
Detailed description of the invention
The present invention is further illustrated below in conjunction with embodiment.
Case study on implementation 1 chitosan oligosaccharide on sexual maturing period and the gestational period Mouse Uterus grow the impact of performance
(1) animal used as test: female and male mouse of kunming, cleaning grade animal, 3 ~ 4 week age, 12 ± 3g, thered is provided by Dalian Medical Univ's Experimental Animal Center, approval number is SCXK (the Liao Dynasty) 2008-0002, and cleaning grade Animal House is raised, temperature 20 ± 1 DEG C, humidity 40% ~ 50%, illumination 12h, pellet is fed, and freely drinks water.Feed mouse perfect compound feed more than 4 weeks, Mouse Weight can reach 26 ± 3g, can be used for mating test.Perfect compound feed composition is in table 1.
(2) experimental technique: choose the female mice that build is similar, energy is active and be divided into 2 groups at random.First group is control group, feeds and does not add the perfect compound feed of chitosan oligosaccharide; Second group is chitosan oligosaccharide, feeds and adds the perfect compound feed of 300mg/kg chitosan oligosaccharide.Meanwhile, male mice is fed and is not added the perfect compound feed of chitosan oligosaccharide.Feed mouse daily ration 6 thoughtful sexual maturing period, select female mice vigorous, be separated organ and also adopt HE colouring method to analyze the feature of Histomorphology.In addition, select female mouse vigorous and male mouse to carry out mating according to 1:1, within the 19th day, add up the shoot formation of female mouse in gestation the 6th day and gestation and adopt HE colouring method to analyze the feature of Histomorphology.
(3) test item: the statistically reproductive performance such as maturity period, gestation the 6th day, gestation the 19th day female mouse uterus index and Histomorphology feature (adopt HE colouring method).
(4) statistical method: the data acquisition SPSSDuncan'smultiplerangetest collected analyzes.
(5) experimental result:
Table 1. experimental basis daily ration composition and nutritional labeling
5.1 chitosan oligosaccharides are on the impact of mouse organs's exponential sum Histomorphology in sexual maturing period
Table 2. chitosan oligosaccharide affected mouse organs's index in sexual maturing period (mg/g)
| Group | Control group | Chitosan oligosaccharide group |
| Female mouse number | N＝10 | N＝10 |
| Female mouse heavy (gram) | 30.85±2.38a | 23.98±2.77b |
| Liver index | 45.9±4.4a | 42.0±5.9ab |
| Index and spleen index | 3.2±0.5a | 2.5±0.4b |
| Renal index | 11.5±1.1a | 11.5±0.9a |
| Ovarian index | 0.6±0.1ab | 0.4±0.1a |
| Fallopian tubal index | 0.17±0.037a | 0.16±0.047a |
| Uterus index | 5.1±1.9a | 4.4±1.4a |
Note: between two groups letter not identical table show significant difference, P<0.05.
From table 2 result, chitosan oligosaccharide group uterus index is difference remarkable (P>0.05) compared with control group.
Fig. 1 result shows that chitosan oligosaccharide improves endometrium of mouse degree of crook in sexual maturing period.Fig. 2 result shows chitosan oligosaccharide raising Mouse Uterus Angiogenesis ability in sexual maturing period and erythrocyte number, and can improve endometrium gauffer degree of crook.
5.2 chitosan oligosaccharides are on the impact of gestation the 6th day mouse organs's exponential sum Histomorphology
Table 3. chitosan oligosaccharide is on gestation mouse organs's index (mg/kg) impact in the 6th day
Note: between two groups letter not identical table show significant difference, P < 0.05.
From table 3 result, chitosan oligosaccharide group Utero-fetus proportion has increase trend (0.73 ± 0.11/0.65 ± 0.19, chitosan oligosaccharide group/control group) (P=0.084) compared with control group.Between two groups, ovarian index and other shoot formation do not have obvious difference (P>0.05).
Fig. 3 result shows that chitosan oligosaccharide improves gestation the 6th day mouse implantation fetus blood vessel number.Fig. 4 result shows that chitosan oligosaccharide improves gestation the 6th day mouse implantation fetus blood vessel number and red blood cell number.
5.3 chitosan oligosaccharides are on the impact of gestation the 19th day mouse organs's exponential sum Histomorphology
Table 4. chitosan oligosaccharide is on gestation mouse organs's index (mg/g) impact in the 19th day
| Project | Control group | Chitosan oligosaccharide group |
| Female mouse number | N＝17 | N＝10 |
| Liver index | 40.4±9.1a | 36.7±7.8a |
| Renal index | 7.9±2.5a | 8.1±1.1a |
| Index and spleen index | 2.3±0.8a | 2.0±0.2a |
| Thymus index | 0.5±0.2a | 0.4±0.1a |
| Ovarian index | 0.3±0.1a | 0.4±0.1a |
| Uterus index | 18.2±5.2a | 18.5±2.8a |
Note: between two groups letter not identical table show significant difference, P<0.05.
From table 4 result, chitosan oligosaccharide group uterus index is difference remarkable (P>0.05) compared with control group.From Fig. 5 result, chitosan oligosaccharide can improve endometrium thickness and endometrium gauffer degree of crook, improves blood vessels of endometrium quantity, and improves uterus muscle thickness (comprising circular muscle and longitudinal muscle) and intramuscular blood vessel number.
Case study on implementation 2 chitosan oligosaccharide grows the impact of performance to weaning period Mouse Uterus
(1) animal used as test: female and male mouse of kunming, cleaning grade animal, 3 ~ 4 week age, 12 ± 3g, thered is provided by Dalian Medical Univ's Experimental Animal Center, approval number is SCXK (the Liao Dynasty) 2008-0002, and cleaning grade Animal House is raised, temperature 20 ± 1 DEG C, humidity 40% ~ 50%, illumination 12h, pellet is fed, and freely drinks water.Feed mouse perfect compound feed more than 4 weeks, Mouse Weight can reach 26 ± 3g, can be used for mating test.Perfect compound feed composition is in table 1.
(2) experimental technique: choose the female mice that build is similar, energy is active and be divided into 2 groups at random.First group is control group, feeds and does not add the perfect compound feed of chitosan oligosaccharide; Second group is chitosan oligosaccharide, feeds and adds the perfect compound feed of 300mg/kg chitosan oligosaccharide.Meanwhile, male mice is fed and is not added the perfect compound feed of chitosan oligosaccharide.Feed mouse daily ration 6 thoughtful sexual maturing period, female mouse vigorous and male mouse is selected to carry out mating according to 1:1, mouse divides the puerperium to farrow, farrowing mouse is continued to the daily ration of feeding containing chitosan oligosaccharide, to wean in the 21st day, be separated weanling female mice organ, statistics shoot formation also adopts HE colouring method to analyze the feature of Histomorphology.
(3) test item: the every only reproductive performance such as female mouse shoot formation and Histomorphology of statistics.And adopt HE colouring method to observe the feature of Histomorphology.
(4) statistical method: the data acquisition SPSSDuncan'smultiplerangetest collected analyzes.
(5) experimental result:
5.1 weaning period shoot formation and Histomorphology feature
Table 5. chitosan oligosaccharide is on female mouse shoot formation (mg/g) impact of weaning period
Note: between two groups letter not identical table show significant difference, P<0.05.
From table 5 result, chitosan oligosaccharide group uterus index and control group comparing difference are not significantly (P>0.05).
Fig. 6 and Fig. 7 result shows that chitosan oligosaccharide can promote weaning period female mouse uterine tissue muscle thickness, Angiogenesis ability and erythrocyte number, and can promote endometrium degree of crook.
Claims (6)
1. functional oligosaccharide is grown in performance at adjustment female uterus and is applied, and it is characterized in that: described functional oligosaccharide is chitosan oligosaccharide, it makes an addition in animal feed, food or medicine as the active ingredient regulating female uterus to grow performance.
2. according to application according to claim 1, it is characterized in that: described functional oligosaccharide makes an addition in jenny perfect compound feed, food or medicine as the active ingredient regulating female uterus to grow performance; Addition in every kilogram of feed, food or medicine is, chitosan oligosaccharide: 10 ~ 1000mg.
3. according to application according to claim 2, it is characterized in that: described functional oligosaccharide can organize myogenesis, Angiogenesis and increase endometrium degree of crook as the active ingredient of feed, food or medicine to improve weaning period female uterus.
4. according to the application described in Claims 2 or 3, it is characterized in that: described functional oligosaccharide as the active ingredient of feed, food or medicine to improve female uterus Angiogenesis and erythrocyte number in sexual maturing period, and can improve endometrium gauffer degree of crook.
5. according to application according to claim 2, it is characterized in that: described functional oligosaccharide can as the active ingredient of feed, food or medicine to improve First Trimester jenny implantation fetal tissue's blood vessel number and red blood cell number.
6. according to application according to claim 2, it is characterized in that: described functional oligosaccharide as the active ingredient of feed, food or medicine to improve latter half of gestation female uterus inner film thickness, gauffer degree of crook and blood vessel number, and can improve uterus muscle thickness and intramuscular blood vessel number.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109198228A (en) * | 2018-11-09 | 2019-01-15 | 佛山市雷米高动物营养保健科技有限公司 | Improve composition, the product and preparation method thereof of pregnant pet reproductive performance |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103875932A (en) * | 2012-12-21 | 2014-06-25 | 中国科学院大连化学物理研究所 | Functional glycoconjugate for improving sow breeding performance |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103875932A (en) * | 2012-12-21 | 2014-06-25 | 中国科学院大连化学物理研究所 | Functional glycoconjugate for improving sow breeding performance |
Non-Patent Citations (2)
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| 唐晓琳等: "壳寡糖螯合锌对雌性昆明小鼠生长和繁殖性能的影响", 《营养学报》 * |
| 王伦学等: "寡糖技术在现代畜牧业上的应用", 《2012新型饲料与饲用抗生素替代品研究应用及产业化发展交流研讨会论文集》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109198228A (en) * | 2018-11-09 | 2019-01-15 | 佛山市雷米高动物营养保健科技有限公司 | Improve composition, the product and preparation method thereof of pregnant pet reproductive performance |
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Application publication date: 20160127 |