CN105250327A - Applications of chitosanoligosaccharide in improvement of animal corpus luteum number, embryo nidation ability and fetal development ability - Google Patents
Applications of chitosanoligosaccharide in improvement of animal corpus luteum number, embryo nidation ability and fetal development ability Download PDFInfo
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- CN105250327A CN105250327A CN201410349055.3A CN201410349055A CN105250327A CN 105250327 A CN105250327 A CN 105250327A CN 201410349055 A CN201410349055 A CN 201410349055A CN 105250327 A CN105250327 A CN 105250327A
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Abstract
The present invention discloses applications of chitosanoligosaccharide in improvement of animal corpus luteum number, embryo nidation ability and fetal development ability. According to the present invention, per 1kg of a feed, food or drug contains 1-800 mg of chitosanoligosaccharide; and the chitosanoligosaccharide is the functional oligosaccharide with effects of improvement of female animal gestation period embryo and fetal growth and development ability, improvement of female animal ovary corpus luteum number, embryo nidation number and live fetal number at the late gestation period, and further has characteristics of no toxic-side effect, no residue, less addition, no pollution, good repeatability, and no adverse impact on the environment.
Description
Technical field
The present invention relates to oligochitosan to apply in raising jenny corpus luteum number, First Trimester Embryo viability and latter half of gestation fetal development ability, specifically, be a kind ofly to be added in animal feed, food or medicine to regulate the functional oligosaccharide of animal reproduction performance, belong to agro-ecology product technique field.
Background technology
China's animal husbandry output value account for 1/3rd of the general production value of agriculture.But China's animal husbandry development is not powerful.Wherein, China's animal varieties depends on import, from the past to now still like this.Reproductive performance is not high is one of principal element of restriction China aquaculture fast development.And the principal element affecting the reproductive performances such as animal litter size is animal number of eggs ovulated, embryo nidation number and latter half of gestation fetal development ability etc.At present, adopt promoting sexual gland hormone if PMSG, HCG, FSH are to promote animal estrus synchronization ovulation in oestrus and embryo nidation clinically, although number of eggs ovulated increases, but the embryo quality of implantation is poor, latter half of gestation fetus absorbance and mortality rate high, cause total yield coefficient not to be significantly improved.Latter half of gestation foetal death and fetal abortion are one of principal elements affecting the reproductive performances such as animal litter size.Research shows that pig occurs dead at pregnancy duration up to 40%-50% embryo and fetus; and show the slow symptom of serious intrauterine fetal growth (IUGR) at latter half of gestation; this is mainly because Placenta Hominis off-capacity causes parent to the nutrition supply limited ability of fetus; foetal growth retardation or death; finally cause the young sum of life birth not high, constrain the large-scale development of aquaculture.Therefore, at latter half of gestation by the growth of nutrition regulation Placenta Hominis and function to improve fetus existence in uterus and developmental capacity, be problem in the urgent need to address.Therefore, need in daily ration, add physiological function and the health status that some active component regulate jenny, improve latter half of gestation fetal development ability, improve latter half of gestation Placenta Hominis supply nutrient substance ability, improve fetal survival number, reduce stillborn fetus number, and improve litter size alive with this.
By the raw material such as shrimp and crab shells, inulin degrade in vitro generate functional oligosaccharide (as oligochitosan, fruit oligose) be widely used in feed industry, these functional sugar content be added in feedstuff are atomic, but the physiological action that they play in animal body is very large, there is energy regulating intestinal canal healthy, promote probiotics growth, suppress harmful intestinal tract bacteria growing, it has the multinomial physiological functions such as antioxidation, blood fat reducing, immunity moderation, penetration enhancement simultaneously, improves the health level of animal from aggregate level.In recent years, functional oligosaccharide becomes the focus in Animal nutrition research, has played good effect in feed industry.But regards functionality oligosaccharide is reported seldom the research that jenny reproductive performance regulates and controls both at home and abroad.
Summary of the invention
It is atomic that the object of the invention is to provide a kind of content, can improve the functional oligosaccharide of jenny corpus luteum number, First Trimester embryo nidation ability and latter half of gestation embryo growth and development ability.
For achieving the above object, the technical solution used in the present invention is:
Using function oligosaccharide of the present invention is oligochitosan, and the oligochitosan added in every 1kg feedstuff, food or medicine is 1 ~ 800mg.
This functional oligosaccharide needs with perfect compound feed used in combination before use.Premix material (as 10% concentration) can be fitted in conventional feed additive, add in perfect compound feed for animal edible.
This functional oligosaccharide feedstuff can improve the fetal development ability of the corpus luteum number of jenny First Trimester, the embryo nidation ability of First Trimester and latter half of gestation well, especially shows animal ovary corpus luteum number, implantation embryo number and lives in the important indicators such as Number of Foetus.
The present invention need weaning period, period of sexual maturity and trimester of pregnancy Long-term Feeding animal.
Advantage of the present invention has: can improve the corpus luteum number of jenny, implantation embryo number and Number of Foetus of living, addition is few, the physiologically active played is high, and continuing use with pregnancy duration between period of sexual maturity can not have a negative impact to environment, is applicable to large-scale application.
Accompanying drawing explanation
Fig. 1. oligochitosan organizes vegf expression level to affect on gestation the 6th day Mouse Uterus;
Fig. 2. oligochitosan is on gestation the 19th day Mouse Uterus, fetus and Placenta Hominis is morphologic affects note: a: matched group uterus fetus and mice; B: matched group uterus fetus; C: matched group fetus; D: matched group Placenta Hominis; E: oligochitosan group uterus fetus and mice; F: oligochitosan group uterus fetus (haircut represent be distorted); G: oligochitosan group fetus; H: oligochitosan group Placenta Hominis;
Fig. 3. oligochitosan affects note to gestation the 19th day Mouse Uterus tissue cross Area morphology: a-c, matched group uterus; D-f, oligochitosan group uterus; BV: blood vessel; RBC: erythrocyte; EM: endometrium; M: Musclar layer;
Fig. 4. oligochitosan affects note to gestation the 19th day mice placenta tissue cross section is morphologic: a-c, matched group Placenta Hominis; D-f, oligochitosan group Placenta Hominis .BV:bloodvessels, blood vessel; RBC, redbloodcell, erythrocyte;
Fig. 5. oligochitosan affects note to gestation the 19th day mice placenta tissue longitudinal section is morphologic: a-c, matched group Placenta Hominis; D-f, oligochitosan group Placenta Hominis .RBC, redbloodcell, erythrocyte.
Detailed description of the invention
The present invention is further illustrated below in conjunction with embodiment.
Case study on implementation 1 oligochitosan is on the impact of the reproductive performances such as mouse ovarian corpus luteum number and implantation embryo number
(1) laboratory animal: female and male mouse of kunming, cleaning grade animal, 3 ~ 4 week age, 12 ± 3g, thered is provided by Dalian Medical Univ's Experimental Animal Center, approval number is SCXK (the Liao Dynasty) 2008-0002, and cleaning grade Animal House is raised, temperature 20 ± 1 DEG C, humidity 40% ~ 50%, illumination 12h, pellet is fed, and freely drinks water.Feed mice perfect compound feed more than 4 weeks, Mouse Weight can reach 26 ± 3g, can be used for mating test.Perfect compound feed composition is in table 1.
(2) experimental technique: choose build similar, the female mice that energy enlivens is divided into two groups at random.First group is matched group, feeds and does not add the perfect compound feed of oligochitosan; Second group is oligochitosan group, feeds and adds the perfect compound feed of 300mg/kg oligochitosan.Meanwhile, male mice is fed and is not added the perfect compound feed of oligochitosan.To feed the thoughtful period of sexual maturity of mice daily ration 6, select female Mus vigorous and male Mus, according to female: property=1:1 carries out copulation, continue to till mice becomes pregnant the 6th day.
(3) test item: the reproductive performances such as statistics every only female Mus ovarian index, uterus index, corpus luteum number, embryo nidation number, detect serum estradiol and progesterone level, adopt HE colouring method to detect oligochitosan to uterus and the morphologic impact of fetus, adopt Westernblotting and immunohistochemical method to detect oligochitosan to the impact of Mouse Uterus VEGF (VEGF) expression.
(4) statistical method: the data acquisition SPSSDuncan'smultiplerangetest collected analyzes.
Table 1.1 experimental basis daily ration composition and nutritional labeling
(5) experimental result:
1.5.1 pregnancy rate
From table 1.2 result, oligochitosan group mice pregnancy rate (70.0%) is compared with matched group (65.6%) does not have obvious difference.
Table 1.2 oligochitosan is on the impact of mice pregnancy rate
Table 1.3 oligochitosan on gestation the 6th day mouse organs's index (mg/kg) impact
Note: shoot formation refers to the ratio of organ weight (mg) and mice weights (g).
1.5.2 shoot formation
From table 1.3 result, oligochitosan to a certain degree improves (P=0.084) Utero-fetus proportion (0.65 ± 0.19/0.73 ± 0.11, matched group/oligochitosan group).Between two groups, ovarian index and other shoot formation do not have notable difference (P>0.05).
1.5.3 corpus luteum number
From table 1.4, table 1.5, table 1.6 result, oligochitosan can improve gestation the 6th day mouse ovarian corpus luteum number 3.73 (23.80/20.07, oligochitosan group/matched group).
Table 1.4 matched group gestation the 6th day mouse ovarian corpus luteum number and follicle number statistical table
Note: Pre-ovu: mature follicle before ovulation; Ovu: follicle of having ovulated; CL: corpus luteum.
Table 1.5 oligochitosan group gestation the 6th day mouse ovarian corpus luteum number and follicle number statistical table
Note: Pre-ovu: mature follicle before ovulation; Ovu: follicle of having ovulated; CL: corpus luteum.
Table 1.6 oligochitosan is on gestation mouse ovarian corpus luteum number (average ± standard deviation) impact in the 6th day
1.5.4 implantation embryo number
Table 1.7 oligochitosan is on the impact of gestation the 6th day mice embryonic implantation number
From table 1.7 and table 1.8 result, oligochitosan to a certain degree improves (P=0.253) gestation the 6th day mice implantation embryo number 1.2 (14.33 ± 2.68/13.11 ± 4.07, oligochitosan group/matched group).
Table 1.8 oligochitosan is on the impact of gestation the 6th day mice embryonic implantation number
1.5.5 mice serum reproductive hormone
From table 1.9, oligochitosan does not affect (P>0.05) gestation the 6th day mice serum estrogen and progesterone level.
Table 1.9 oligochitosan is on gestation the 6th day mice serum estrogen and progesterone level impact
1.5.6 Mouse Uterus organizes vegf expression level
As seen from Figure 1 (Westernblotting), oligochitosan improves gestation the 6th day Mouse Uterus tissue blood vessel endothelial cell growth factor (ECGF) (VEGF) expression.
Case study on implementation 2 oligochitosan is on the impact of mouse pregnancy later stage reproductive performance
(1) laboratory animal: female and male mouse of kunming, cleaning grade animal, 3 ~ 4 week age, 12 ± 3g, thered is provided by Dalian Medical Univ's Experimental Animal Center, approval number is SCXK (the Liao Dynasty) 2008-0002, and cleaning grade Animal House is raised, temperature 20 ± 1 DEG C, humidity 40% ~ 50%, illumination 12h, pellet is fed, and freely drinks water.Feed mice perfect compound feed more than 4 weeks, Mouse Weight can reach 26 ± 3g, can be used for mating test.Perfect compound feed composition is in table 1.
(2) experimental technique: choose build similar, the female mice that energy enlivens is divided into 2 groups at random.First group is matched group, feeds and does not add the perfect compound feed of oligochitosan; Second group is oligochitosan, feeds and adds the perfect compound feed of 30mg/kg oligochitosan.Meanwhile, male mice is fed and is not added the perfect compound feed of oligochitosan.To feed the thoughtful period of sexual maturity of mice daily ration 6, select female Mus vigorous and male Mus, according to female: property=1:1 carries out copulation, continue to mouse pregnancy till the 19th day.
(3) test item: the every only female Mus ovarian index of statistics, uterus index, fetus sum, the reproductive performance such as Number of Foetus, fetus/Placenta Hominis of living, adopt HE colouring method to detect oligochitosan to uterus and the morphologic impact of fetus, adopt Westernblotting and immunohistochemical method to detect oligochitosan to the impact of the expressions such as Mouse Uterus VEGF (VEGF).
(4) statistical method: the data acquisition SPSSDuncan'smultiplerangetest collected analyzes.
(5) experimental result:
Table 2.1 experimental basis daily ration composition and nutritional labeling
2.5.1 pregnancy rate
Table 2.2 oligochitosan is on the impact of mice pregnancy rate
From table 2.2 result, oligochitosan group female Mus pregnancy rate (73.91%) (80.00%) compared with matched group does not have obvious difference.Wherein, oligochitosan group has 5 mice generation premature labors.
2.5.2 mouse fetal number
From table 2.3, table 2.4 and table 2.5 result, oligochitosan to a certain degree improves (P=0.060) mice lives Number of Foetus 1.5 (14.30 ± 1.06/12.82 ± 2.21, oligochitosan group/matched group).
Table 2.3 matched group gestation the 19th day mouse fetal number grows statistical table
Table 2.4 oligochitosan group mouse pregnancy the 19th day Number of Foetus grows statistical table
Table 2.5 oligochitosan is on mouse pregnancy Number of Foetus impact in the 19th day
2.5.3 reproductive performance
From table 2.6 result, oligochitosan obviously (P=0.004) improves gestation the 19th day mouse fetal-uterus proportion (39.04 ± 1.64/33.50 ± 4.40%, oligochitosan group/matched group), and to a certain degree (P=0.282) improves Placental Efficiency (10.31 ± 0.55/9.92 ± 2.21, oligochitosan group/matched group).As seen from Figure 2, in oligochitosan group, have the uterus of more ratio to be distorted, this intrauterine contains more fetus.
Table 2.6 oligochitosan is on the impact of gestation the 19th day MOUSE REPRODUCTION performance
2.5.4 oligochitosan is on gestation the 19th day Mouse Uterus and the morphologic impact of placenta tissue
By Fig. 3 result visible (HE dyeing), oligochitosan can improve endometrium of mouse thickness, gauffer degree of crook and blood vessel number, and improves muscle thickness and intramuscular blood vessel number.
By Fig. 4 (Placenta Hominis cross section) visible (HE dyeing), oligochitosan adds gestation the 19th day mice placenta tissue blood vessel number and RBC number.By Fig. 5 (Placenta Hominis longitudinal section) visible (HE dyeing), oligochitosan adds gestation the 19th day mice placenta tissue RBC number.
Claims (6)
1. oligochitosan is applied in raising animal corpus luteum number, embryo nidation, fetal development ability, it is characterized in that: oligochitosan makes an addition in feedstuff, food or medicine as the active ingredient of adjustment jenny reproductive performance, raising fetal development ability.
2. according to application according to claim 1, it is characterized in that: described functional oligosaccharide makes an addition in jenny perfect compound feed as regulating the active ingredient of jenny first half period of gestation corpus luteum number, implantation embryo number and latter half of gestation Number of Foetus alive; It can improve corpus luteum number, implantation embryo number, Number of Foetus of living; Addition in every kilogram of feed, food or medicine is: oligochitosan 1 ~ 800mg.
3. according to the application described in claim 1 or 2, it is characterized in that: described functional oligosaccharide can as the effective ingredient of feedstuff, food or medicine to improve the corpus luteum number of female ovarium.
4. according to the application described in claim 1 or 2, it is characterized in that: described functional oligosaccharide can as the effective ingredient of feedstuff, food or medicine to improve the implantation embryo number of jenny First Trimester.
5. according to the application described in claim 1 or 2, it is characterized in that: described functional oligosaccharide can as the effective ingredient of feedstuff, food or medicine to improve the Number of Foetus alive of jenny latter half of gestation.
6. according to the application described in claim 1 or 2, it is characterized in that: described functional oligosaccharide can as the effective ingredient of feedstuff, food or medicine to improve jenny latter half of gestation Placenta Hominis blood vessel number and RBC number.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116473164A (en) * | 2023-06-21 | 2023-07-25 | 北京市农林科学院 | Application of chenodeoxycholic acid in preparation of product for increasing number of animal implantation sites |
| CN116831222A (en) * | 2023-07-07 | 2023-10-03 | 华南农业大学 | Application of fructooligosaccharide as additive in preparation of products for relieving bad gestation fates of sows and weaning diarrhea of piglets |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103875932A (en) * | 2012-12-21 | 2014-06-25 | 中国科学院大连化学物理研究所 | Functional glycoconjugate for improving sow breeding performance |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103875932A (en) * | 2012-12-21 | 2014-06-25 | 中国科学院大连化学物理研究所 | Functional glycoconjugate for improving sow breeding performance |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116473164A (en) * | 2023-06-21 | 2023-07-25 | 北京市农林科学院 | Application of chenodeoxycholic acid in preparation of product for increasing number of animal implantation sites |
| CN116473164B (en) * | 2023-06-21 | 2023-11-03 | 北京市农林科学院 | Application of chenodeoxycholic acid in preparation of product for increasing number of animal implantation sites |
| CN116831222A (en) * | 2023-07-07 | 2023-10-03 | 华南农业大学 | Application of fructooligosaccharide as additive in preparation of products for relieving bad gestation fates of sows and weaning diarrhea of piglets |
| CN116831222B (en) * | 2023-07-07 | 2024-06-11 | 华南农业大学 | Application of fructooligosaccharide as additive in preparation of products for enhancing placenta barrier function of pregnant sows |
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Application publication date: 20160120 |