CN105238694B - A kind of method that hailian seaweed control unarmored dinoflagellate outburst is cultivated in grouper nursery pond - Google Patents
A kind of method that hailian seaweed control unarmored dinoflagellate outburst is cultivated in grouper nursery pond Download PDFInfo
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- CN105238694B CN105238694B CN201510543901.XA CN201510543901A CN105238694B CN 105238694 B CN105238694 B CN 105238694B CN 201510543901 A CN201510543901 A CN 201510543901A CN 105238694 B CN105238694 B CN 105238694B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The present invention provides in a kind of grouper nursery pond cultivate hailian seaweed control unarmored dinoflagellate outburst method, include the following steps, 1) cultivating pool is scrubbed clean after, add seawater；2) water disinfection is carried out using calcium hypochlorite, sodium thiosulfate neutralisation treatment is used after handling 8~16h, and be aerated 18~30h, treated that the chlorine in water all disappears, add nutritive salt, the hailian seaweed of laboratory cultures is expanded in cultivating pool step by step and is cultivated；3) hailian seaweed is cultivated in cultivating pool after 4~8 days, launches grouper fish-egg.According to important function of the hailian seaweed during aquatic product animal raise seedling, the more purebred hailian seaweed of cement pit culture indoors.Using the outburst of hailian seaweed control unarmored dinoflagellate in the grouper nursery pond, cost is low, operating method is simple and technology is easy to grasp；The ecosystem can keep stable for a long time in culturing pool, improve survival rate of seedling, remarkable in economical benefits, has broad application prospects.
Description
Technical field
The invention belongs to aquaculture field, and more particularly, to being cultivated in a kind of grouper nursery pond, hailian seaweed control is naked
The method of dinoflagellate outburst.
Background technology
Unarmored dinoflagellate is under the jurisdiction of Pyrrhophyta, Dinophyceae, unarmored dinoflagellate section, Gymnodinium, and most species are moved in seawater.Its
Body is small, full of nutrition, breeding is rapid, and a variety of toxin can be produced by own metabolism during its growth is bred, also,
Water body color can be changed during unarmored dinoflagellate amount reproduction, larger harm is caused to aquatic livestock.
Hailian seaweed is as shellfish, the direct open-mouthed bait of prawn larvae and the part fish young and culture wheel animalcule, artemia, oar
The fortification food of the secondary food organisms such as sufficient class, can not be substituted in the status of aquatic fry growing.As wild algae hailian seaweed
The advantages of adaptable strong, also, after hailian seaweed becomes advantage algae in water body, can effectively suppress other algae
Growth.
The content of the invention
In view of this, the present invention is directed to propose cultivating hailian seaweed control unarmored dinoflagellate outburst in a kind of grouper nursery pond
Method, this method cost is low, operating method is simple and technology is easy to grasp, and effectively avoids unarmored dinoflagellate in breeding water body
Outburst.
To reach above-mentioned purpose, the technical proposal of the invention is realized in this way：
A kind of method that hailian seaweed control unarmored dinoflagellate outburst is cultivated in grouper nursery pond, includes the following steps,
1) after cultivating pool is scrubbed clean, seawater is added；
2) water disinfection is carried out using calcium hypochlorite, uses sodium thiosulfate neutralisation treatment after handling 8~16h, and be aerated 18
~30h, treats that the chlorine in water all disappears, adds nutritive salt, hailian seaweed is inoculated into cultivating pool and is cultivated；Preferably, extra large chain
Algae is the purebred hailian seaweed by laboratory cultures；Preferably, the hailian seaweed cultivated in triangular flask is spread cultivation by bait bucket, most
After be inoculated into cultivating pool and cultivate；
3) hailian seaweed is cultivated in cultivating pool after 4~8 days, water is in taupe, and hailian seaweed density reaches 50,000/mL,
Launch grouper fish-egg；Preferably, the density of grouper fish-egg is 0.2~1.2 ten thousand/m3；Preferably, 10,000/m3。
Preferably, in step 2), final concentration of 18~22ppm of chlorion；Add sodium thiosulfate in and chlorion, its
The ratio between the final concentration of middle chlorion and the final concentration of sodium thiosulfate are 1:1~3:1；Preferably, chlorion is final concentration of
20ppm, the ratio between the final concentration of chlorion and the final concentration of sodium thiosulfate are 2:1.
Preferably, in step 3), in cultivating pool cultivating hailian seaweed started into 22~26h this periods, uninterruptedly
Aeration.
Preferably, in step 2), the nutritive salt includes ammonium nitrate, sodium dihydrogen phosphate, ironic citrate, sodium metasilicate；Will
After nutritive salt is added to seawater, ammonium nitrate, sodium dihydrogen phosphate, ironic citrate, the concentration of sodium metasilicate each component is respectively 6~
14mg/L, 1~3mg/L, 0.05~0.35mg/L, 30~50mg/L
Preferably, in step 2), the nutritive salt includes ammonium nitrate, sodium dihydrogen phosphate, ironic citrate, sodium metasilicate；Will
After nutritive salt is added to seawater, ammonium nitrate, sodium dihydrogen phosphate, ironic citrate, the concentration of sodium metasilicate each component is respectively 8~
12mg/L, 1.5~2.5mg/L, 0.1~0.35mg/L, 35~45mg/L.
Preferably, in step 2), the nutritive salt includes ammonium nitrate, sodium dihydrogen phosphate, ironic citrate, sodium metasilicate；Will
After nutritive salt is added to seawater, ammonium nitrate, sodium dihydrogen phosphate, ironic citrate, the concentration of sodium metasilicate each component be respectively 10mg/L,
2mg/L、0.2mg/L、40mg/L。
Preferably, the nutritive salt is fully dissolved by being heated to 55~65 DEG C of seawater, it is preferred that with 60 DEG C
Seawater dissolves.
Preferably, in step 3), after launching grouper fish-egg, the density of hailian seaweed maintains 3.5~60,000 in cultivating pool
A cell/mL.
Preferably, in step 3), after launching grouper fish-egg, the density of hailian seaweed maintains 4~50,000 in cultivating pool
Cell/mL.
It is quick-fried that hailian seaweed control unarmored dinoflagellate is cultivated relative to the prior art, in a kind of grouper nursery pond of the present invention
The method of hair, has the advantage that：According to important function of the hailian seaweed during aquatic product animal raise seedling, indoors cement pit training
Support more purebred hailian seaweed.Using the outburst of hailian seaweed control unarmored dinoflagellate in the grouper nursery pond, cost is low, operation
Method is simple and technology is easy to grasp；Culturing pool yellowish-brown, transparency is moderate, and the ecosystem can keep steady for a long time
It is fixed, survival rate of seedling is improved, remarkable in economical benefits, has broad application prospects.
Embodiment
Below will be by embodiment come the present invention will be described in detail.
Embodiment one
Floor space 25m for the cultivating pool of grouper nursery2, pond depth 2m, adds after cultivating pool is scrubbed clean
Seawater, depth 1.5m.Water disinfection is carried out using calcium hypochlorite (using final concentration of 20ppm), thio sulphur is used after handling 12h
Sour sodium (uses final concentration of 8g/cm3) neutralisation treatment and constantly sudden and violent gas 24h.Treat that chlorine all disappears in water, add nutritive salt, battalion
Support salt fully to dissolve using the seawater for being heated to 60 DEG C, at this time ammonium nitrate, sodium dihydrogen phosphate, ironic citrate, silicon in cultivating pool
The concentration of sour sodium each component is respectively 10mg/L, 2mg/L, 0.2mg/L, 40mg/L.The hailian seaweed of laboratory cultures is expanded step by step
Cultivated into cultivating pool greatly.In incubation it is preceding 24 it is small when uninterruptedly inflate, prevent frond from sinking；Cultivated in cultivating pool
After hailian seaweed 5 days, grouper fish-egg is then launched, after launching grouper fish-egg, the hailian seaweed of control unarmored dinoflagellate outburst is in nursery
The density in pond maintains ten thousand cell/mL of 4.8-5.
Embodiment two
Area 25m for the cultivating pool of grouper nursery2, pond depth 2m, adds sea after cultivating pool is scrubbed clean
Water, depth 1.6m.Water disinfection is carried out using calcium hypochlorite (using final concentration of 20ppm), thiosulfuric acid is used after handling 10h
Sodium (uses final concentration of 8g/cm3) neutralisation treatment and constantly sudden and violent gas 20h.Treat that chlorine all disappears in water, adds nutritive salt, nutrition
Salt is fully dissolved using the seawater for being heated to 60 DEG C, at this time ammonium nitrate, sodium dihydrogen phosphate, ironic citrate, silicic acid in cultivating pool
The concentration of sodium each component is respectively 7mg/L, 1.4mg/L, 0.1mg/L, 34mg/L.The hailian seaweed of laboratory cultures is expanded step by step
Cultivated into cultivating pool.In incubation it is preceding 22 it is small when uninterruptedly inflate, prevent frond from sinking；Sea is cultivated in cultivating pool
After chain algae 7 days, grouper fish-egg is then launched, density of the hailian seaweed that control unarmored dinoflagellate is broken out in nursery pond maintains 4.3-
4.5 ten thousand cell/mL.
Embodiment three
Area 25m for the cultivating pool of grouper nursery2, pond depth 2m, adds sea after cultivating pool is scrubbed clean
Water, depth 1.6m.Water disinfection is carried out using calcium hypochlorite (using final concentration of 20ppm), thiosulfuric acid is used after handling 10h
Sodium (uses final concentration of 8g/cm3) neutralisation treatment and constantly sudden and violent gas 26h.Treat that chlorine all disappears in water, adds nutritive salt, nutrition
Salt is fully dissolved using the seawater for being heated to 60 DEG C, at this time ammonium nitrate, sodium dihydrogen phosphate, ironic citrate, silicic acid in cultivating pool
The concentration of sodium each component is respectively 11mg/L, 2.2mg/L, 0.15mg/L, 44mg/L.The hailian seaweed of laboratory cultures is expanded step by step
Cultivated into cultivating pool greatly.In incubation it is preceding 26 it is small when uninterruptedly inflate, prevent frond from sinking；Cultivated in cultivating pool
After hailian seaweed 6 days, grouper fish-egg is then launched, density of the hailian seaweed that control unarmored dinoflagellate is broken out in nursery pond maintains
4.5-4.7 ten thousand cell/mL.
Contrast experiment, the area 25m for the cultivating pool of grouper nursery2, pond depth 2m, cultivating pool is scrubbed clean
After add seawater, depth 1.6m, launches grouper fish-egg.
One~embodiment of embodiment three and the density of the unarmored dinoflagellate of contrast experiment are detected, it is as shown in the table.
| First day | 5th day | Tenth day | 15th day | Ahau | |
| Embodiment one | 0 cell/mL | 0 cell/mL | 33 cell/mL | 33 cell/mL | 33 cell/mL |
| Embodiment two | 0 cell/mL | 33 cell/mL | 133 cell/mL | 133 cell/mL | 133 cell/mL |
| Embodiment three | 0 cell/mL | 33 cell/mL | 133 cell/mL | 233 cell/mL | 233 cell/mL |
| Contrast experiment | 0 cell/mL | 233 cell/mL | 1700 cell/mL | 5400 cell/mL | 750 cell/mL |
Meanwhile observe the hatching situation of grouper fish-egg, the results show：Grouper fish-egg can be incubated normally in embodiment one
Change (incubation rate 78%)；The incubation rate of grouper fish-egg is less than example 1, its incubation rate difference in embodiment two, embodiment three
For：62%th, 53%；And control experiment group is influenced not hatch success by unarmored dinoflagellate outburst.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
With within principle, any modification, equivalent replacement, improvement and so on, should all be included in the protection scope of the present invention god.
Claims (13)
1. the method for hailian seaweed control unarmored dinoflagellate outburst is cultivated in a kind of grouper nursery pond, it is characterised in that：Including as follows
Step,
1) after cultivating pool is scrubbed clean, seawater is added；
2) water disinfection is carried out using calcium hypochlorite, uses sodium thiosulfate neutralisation treatment after handling 8~16h, the end of chlorion is dense
Spend for 18~22ppm；Add in sodium thiosulfate and chlorion, the wherein final concentration of the final concentration of chlorion and sodium thiosulfate
The ratio between be 1:1~3:1；And 18~30h is aerated, nutritive salt is added, the nutritive salt includes ammonium nitrate, sodium dihydrogen phosphate, lemon
Lemon acid iron, sodium metasilicate；After nutritive salt is added to seawater, ammonium nitrate, sodium dihydrogen phosphate, ironic citrate, sodium metasilicate each component
Concentration is respectively 6~14mg/L, 1~3mg/L, 0.05~0.35mg/L, 30~50mg/L；Hailian seaweed is inoculated into cultivating pool
Middle culture；
3) cultivate hailian seaweed after 4~8 days in cultivating pool, water is in taupe, hailian seaweed density reach 3.5~50,000/
ML, launches grouper fish-egg.
2. cultivating the method for hailian seaweed control unarmored dinoflagellate outburst in grouper nursery pond according to claim 1, it is special
Sign is：In step 2), hailian seaweed is the purebred hailian seaweed by laboratory cultures.
3. cultivating the method for hailian seaweed control unarmored dinoflagellate outburst in grouper nursery pond according to claim 1, it is special
Sign is：In step 2), the hailian seaweed cultivated in triangular flask is spread cultivation by bait bucket, is finally inoculated into cultivating pool and trains
Support.
4. cultivating the method for hailian seaweed control unarmored dinoflagellate outburst in grouper nursery pond according to claim 1, it is special
Sign is：In step 3), the density of grouper fish-egg is 0.2~1.2 ten thousand/m3。
5. cultivating the method for hailian seaweed control unarmored dinoflagellate outburst in grouper nursery pond according to claim 1, it is special
Sign is：In step 3), the density of grouper fish-egg is 10,000/m3。
6. cultivating the method for hailian seaweed control unarmored dinoflagellate outburst in grouper nursery pond according to claim 1, it is special
Sign is：In step 2), the final concentration of 20ppm of chlorion, the ratio between the final concentration of chlorion and the final concentration of sodium thiosulfate
For 2:1.
7. cultivating the method for hailian seaweed control unarmored dinoflagellate outburst in grouper nursery pond according to claim 1, it is special
Sign is：In step 3), in cultivating pool cultivating hailian seaweed started into 22~26h this periods, uninterrupted aeration.
8. cultivating the method for hailian seaweed control unarmored dinoflagellate outburst in grouper nursery pond according to claim 1, it is special
Sign is：In step 2), the nutritive salt includes ammonium nitrate, sodium dihydrogen phosphate, ironic citrate, sodium metasilicate；By nutritive salt plus
Enter to after seawater, ammonium nitrate, sodium dihydrogen phosphate, ironic citrate, the concentration of sodium metasilicate each component be respectively 8~12mg/L, 1.5~
2.5mg/L, 0.1~0.35mg/L, 35~45mg/L.
9. cultivating the method for hailian seaweed control unarmored dinoflagellate outburst in grouper nursery pond according to claim 1, it is special
Sign is：In step 2), the nutritive salt includes ammonium nitrate, sodium dihydrogen phosphate, ironic citrate, sodium metasilicate；By nutritive salt plus
Enter to after seawater, ammonium nitrate, sodium dihydrogen phosphate, ironic citrate, the concentration of sodium metasilicate each component be respectively 10mg/L, 2mg/L,
0.2mg/L、40mg/L。
10. the method for hailian seaweed control unarmored dinoflagellate outburst is cultivated in grouper nursery pond according to claim 8 or claim 9,
It is characterized in that：The nutritive salt is fully dissolved by being heated to 55~65 DEG C of seawater.
11. the method for hailian seaweed control unarmored dinoflagellate outburst is cultivated in grouper nursery pond according to claim 8 or claim 9,
It is characterized in that：The nutritive salt is fully dissolved by being heated to 60 DEG C of seawater.
12. the method for hailian seaweed control unarmored dinoflagellate outburst is cultivated in grouper nursery pond according to claim 10, its
It is characterized in that：In step 3), after launching grouper fish-egg, the density of hailian seaweed maintains 3.5~60,000 thin in cultivating pool
Born of the same parents/mL.
13. the method for hailian seaweed control unarmored dinoflagellate outburst is cultivated in grouper nursery pond according to claim 10, its
It is characterized in that：In step 3), launch grouper fish-egg after, in cultivating pool the density of hailian seaweed maintain 4~50,000 cells/
mL。
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| CN111183930A (en) * | 2020-02-25 | 2020-05-22 | 海南晨海水产有限公司 | Algae cultivation and water fertilization method for outdoor ecological breeding of grouper |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| JPH09173050A (en) * | 1995-12-22 | 1997-07-08 | Chikyu Kankyo Sangyo Gijutsu Kenkyu Kiko | Culture of microalgae belonging to green algae |
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| CN101647406B (en) * | 2009-09-04 | 2011-09-28 | 福建蓝鲸水产有限公司 | Benthic diatom culture method for growing seedlings and abalone fry culture method |
| CN101836616A (en) * | 2010-06-17 | 2010-09-22 | 广西红榄科技有限公司 | Pollution-free healthy culture method of grouper in pond |
| CN101926298A (en) * | 2010-07-29 | 2010-12-29 | 广东海洋大学 | Method for constructing specific pathogen-free penaeid shrimp culture system |
| CN103004662B (en) * | 2012-12-21 | 2014-04-09 | 浙江省淡水水产研究所 | Method for cultivating loach water flower seedlings by using fresh water benthic diatoms |
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| JPH09173050A (en) * | 1995-12-22 | 1997-07-08 | Chikyu Kankyo Sangyo Gijutsu Kenkyu Kiko | Culture of microalgae belonging to green algae |
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