CN105238694A - Method for controlling Gymnodinium aerucyinosum outbreak by culturing Thalassiosira in grouper nursery pond - Google Patents
Method for controlling Gymnodinium aerucyinosum outbreak by culturing Thalassiosira in grouper nursery pond Download PDFInfo
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- CN105238694A CN105238694A CN201510543901.XA CN201510543901A CN105238694A CN 105238694 A CN105238694 A CN 105238694A CN 201510543901 A CN201510543901 A CN 201510543901A CN 105238694 A CN105238694 A CN 105238694A
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- cabrilla
- hailian seaweed
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention provides a method for controlling Gymnodinium aerucyinosum outbreak by culturing Thalassiosira in a grouper nursery pond. The method includes the steps of 1, brushing the culture pond, and adding sea water; 2, sterilizing pond water with calcium hypochlorite for 8-16 h, neutralizing the pond water with sodium thiosulfate, aerating the pond water for 18-30 h, adding a nutrient salt after chlorine in the water disappears completely, amplifying step by step Thalassiosira cultured in a lab, and culturing Thalassiosira in the culture pond; 3, culturing Thalassiosira in the culture pond for 4-8 days, and adding eggs of groupers. According to the important action of Thalassiosira in the aquatic nursery process, Thalassiosira of pure breeds are cultured in the indoor cement pond. The outbreak of Gymnodinium aerucyinosum in the grouper nursery pond is controlled by using Thalassiosira, the cost is low, an operating method is simple, and the technique is easy to master; an ecosystem in the culture pond water may stay stable for a long time, nursery survival rate is increased, economic benefit is significant, and the method has a promising application prospect.
Description
Technical field
The invention belongs to aquaculture field, especially relate in a kind of cabrilla nursery pond and cultivate the method that hailian seaweed controls unarmored dinoflagellate outburst.
Background technology
Unarmored dinoflagellate is under the jurisdiction of Pyrrophyta, Dinophyceae, unarmored dinoflagellate section, Gymnodinium, and most kind moves in seawater.Its individuality is small, nutritious, breeding is rapid, in the process of its growth and breeding, produce multiple toxin by own metabolism, and, water body color can be changed during unarmored dinoflagellate amount reproduction, larger harm is caused to aquatic animal.
Hailian seaweed as shellfish, prawn larvae and the part fish young direct open-mouthed bait and cultivate the nutritional fortification food of the secondary food organism such as wheel animalcule, halogen worm, copepods, can not be substituted in the status of aquatic fry growing.As wild algae kind hailian seaweed, there is adaptable advantage, and, when hailian seaweed becomes in water body after advantage algae kind, the growth of other algae effectively can be suppressed.
Summary of the invention
In view of this, the present invention is intended to propose to cultivate the method that hailian seaweed controls unarmored dinoflagellate outburst in a kind of cabrilla nursery pond, and the method cost is low, working method simple and technology is easy to grasp, and effectively avoids the outburst of unarmored dinoflagellate in aquaculture water.
For achieving the above object, technical scheme of the present invention is achieved in that
Cultivate the method that hailian seaweed controls unarmored dinoflagellate outburst in cabrilla nursery pond, comprise the steps,
1), after being scrubbed clean by cultivating pool, seawater is added;
2) use calcium hypochlorite to carry out pond water sterilization, after process 8 ~ 16h, use Sulfothiorine neutralizing treatment, and aeration 18 ~ 30h, treat that the chlorine in water all disappears, add nutritive salt, hailian seaweed is inoculated in cultivating pool and cultivates; Preferably, hailian seaweed is the purebred hailian seaweed through laboratory culture; Preferably, the hailian seaweed cultivated in triangular flask is spread cultivation by bait bucket, be finally inoculated in cultivating pool and cultivate;
3) in cultivating pool, cultivate hailian seaweed after 4 ~ 8 days, Chi Shui is taupe, and hailian seaweed density reaches 50,000/ml, throws in cabrilla fish-egg; Preferably, the density of cabrilla fish-egg is 0.2 ~ 1.2 ten thousand/m
3; Preferably, 10,000/m
3.
Preferably, step 2) in, the final concentration of chlorion is 18 ~ 22ppm; Add in Sulfothiorine and chlorion, wherein the final concentration of chlorion is 1:1 ~ 3:1 with the ratio of the final concentration of Sulfothiorine; Preferably, the final concentration of chlorion is 20ppm, and the final concentration of chlorion is 2:1 with the ratio of the final concentration of Sulfothiorine.
Preferably, step 3) in, cultivate in cultivating pool hailian seaweed start to 22 ~ 26h during this period of time in, uninterrupted aeration.
Preferably, step 2) in, described nutritive salt comprises ammonium nitrate, SODIUM PHOSPHATE, MONOBASIC, ironic citrate, water glass; After nutritive salt is joined seawater, the concentration of each component of ammonium nitrate, SODIUM PHOSPHATE, MONOBASIC, ironic citrate, water glass is respectively 6 ~ 14mg/L, 1 ~ 3mg/L, 0.05 ~ 0.35mg/L, 30 ~ 50mg/L
Preferably, step 2) in, described nutritive salt comprises ammonium nitrate, SODIUM PHOSPHATE, MONOBASIC, ironic citrate, water glass; After nutritive salt is joined seawater, the concentration of each component of ammonium nitrate, SODIUM PHOSPHATE, MONOBASIC, ironic citrate, water glass is respectively 8 ~ 12mg/L, 1.5 ~ 2.5mg/L, 0.1 ~ 0.35mg/L, 35 ~ 45mg/L.
Preferably, step 2) in, described nutritive salt comprises ammonium nitrate, SODIUM PHOSPHATE, MONOBASIC, ironic citrate, water glass; After nutritive salt is joined seawater, the concentration of each component of ammonium nitrate, SODIUM PHOSPHATE, MONOBASIC, ironic citrate, water glass is respectively 10mg/L, 2mg/L, 0.2mg/L, 40mg/L.
Preferably, described nutritive salt is that the seawater by being heated to 55 ~ 65 DEG C fully dissolves, and preferably, dissolves with the seawater of 60 DEG C.
Preferably, step 3) in, after throwing in cabrilla fish-egg, in cultivating pool, the density of hailian seaweed maintains 3.5 ~ 60,000 cell/mL.
Preferably, step 3) in, after throwing in cabrilla fish-egg, in cultivating pool, the density of hailian seaweed maintains 4 ~ 50,000 cell/mL.
Relative to prior art, the method that hailian seaweed controls unarmored dinoflagellate outburst is cultivated in a kind of cabrilla nursery pond of the present invention, there is following advantage: according to the vital role of hailian seaweed in aquatic product animal raise seedling process, cultivate more purebred hailian seaweed at indoor water mud sump.Utilize hailian seaweed to control the outburst of unarmored dinoflagellate in described cabrilla nursery pond, cost is low, working method is simple and technology is easy to grasp; Culturing pool tawny, transparency is moderate, and the ecosystem can keep stable for a long time, improves survival rate of seedling, remarkable in economical benefits, has broad application prospects.
Embodiment
The present invention will be described in detail below by embodiment.
Embodiment one
For the floorage 25m of the cultivating pool of cabrilla nursery
2, the dark 2m in pond, adds seawater after being scrubbed clean by cultivating pool, and the degree of depth is 1.5m.Use calcium hypochlorite (use final concentration is 20ppm) to carry out pond water sterilization, (use final concentration is 8g/cm to use Sulfothiorine after process 12h
3) neutralizing treatment also continuous sudden and violent gas 24h.Treat that in water, chlorine all disappears, add nutritive salt, nutritive salt uses the seawater being heated to 60 DEG C fully to dissolve, and now in cultivating pool, the concentration of ammonium nitrate, SODIUM PHOSPHATE, MONOBASIC, ironic citrate, each component of water glass is respectively 10mg/L, 2mg/L, 0.2mg/L, 40mg/L.The hailian seaweed of laboratory culture is expanded in cultivating pool step by step and cultivates.In culturing process, uninterruptedly inflation in first 24 hours, prevents frond from sinking; In cultivating pool, cultivate hailian seaweed after 5 days, then throw in cabrilla fish-egg, after throwing in cabrilla fish-egg, the hailian seaweed controlling unarmored dinoflagellate outburst maintains 4.8-5 ten thousand cell/ml in the density in nursery pond.
Embodiment two
For the area 25m of the cultivating pool of cabrilla nursery
2, the dark 2m in pond, adds seawater after being scrubbed clean by cultivating pool, and the degree of depth is 1.6m.Use calcium hypochlorite (use final concentration is 20ppm) to carry out pond water sterilization, (use final concentration is 8g/cm to use Sulfothiorine after process 10h
3) neutralizing treatment also continuous sudden and violent gas 20h.Treat that in water, chlorine all disappears, add nutritive salt, nutritive salt uses the seawater being heated to 60 DEG C fully to dissolve, and now in cultivating pool, the concentration of ammonium nitrate, SODIUM PHOSPHATE, MONOBASIC, ironic citrate, each component of water glass is respectively 7mg/L, 1.4mg/L, 0.1mg/L, 34mg/L.The hailian seaweed of laboratory culture is expanded in cultivating pool step by step and cultivates.In culturing process, uninterruptedly inflation in first 22 hours, prevents frond from sinking; In cultivating pool, cultivate hailian seaweed after 7 days, then throw in cabrilla fish-egg, the hailian seaweed controlling unarmored dinoflagellate outburst maintains 4.3-4.5 ten thousand cell/ml in the density in nursery pond.
Embodiment three
For the area 25m of the cultivating pool of cabrilla nursery
2, the dark 2m in pond, adds seawater after being scrubbed clean by cultivating pool, and the degree of depth is 1.6m.Use calcium hypochlorite (use final concentration is 20ppm) to carry out pond water sterilization, (use final concentration is 8g/cm to use Sulfothiorine after process 10h
3) neutralizing treatment also continuous sudden and violent gas 26h.Treat that in water, chlorine all disappears, add nutritive salt, nutritive salt uses the seawater being heated to 60 DEG C fully to dissolve, and now in cultivating pool, the concentration of ammonium nitrate, SODIUM PHOSPHATE, MONOBASIC, ironic citrate, each component of water glass is respectively 11mg/L, 2.2mg/L, 0.15mg/L, 44mg/L.The hailian seaweed of laboratory culture is expanded in cultivating pool step by step and cultivates.In culturing process, uninterruptedly inflation in first 26 hours, prevents frond from sinking; In cultivating pool, cultivate hailian seaweed after 6 days, then throw in cabrilla fish-egg, the hailian seaweed controlling unarmored dinoflagellate outburst maintains 4.5-4.7 ten thousand cell/ml in the density in nursery pond.
Contrast experiment, for the area 25m of the cultivating pool of cabrilla nursery
2, the dark 2m in pond, adds seawater after being scrubbed clean by cultivating pool, and the degree of depth is 1.6m, throws in cabrilla fish-egg.
Detect the density of the unarmored dinoflagellate of embodiment one ~ embodiment three and contrast experiment, as shown in the table.
| First day | 5th day | Tenth day | 15 day | Ahau | |
| Embodiment one | 0 cell/ml | 0 cell/ml | 33 cell/ml | 33 cell/ml | 33 cell/ml |
| Embodiment two | 0 cell/ml | 33 cell/ml | 133 cell/ml | 133 cell/ml | 133 cell/ml |
| Embodiment three | 0 cell/ml | 33 cell/ml | 133 cell/ml | 233 cell/ml | 233 cell/ml |
| Contrast experiment | 0 cell/ml | 233 cell/ml | 1700 cell/ml | 5400 cell/ml | 750 cell/ml |
Meanwhile, observe the hatching situation of cabrilla fish-egg, result shows: in embodiment one, cabrilla fish-egg can normal incubation (hatching rate is 78%); In embodiment two, embodiment three, the hatching rate of cabrilla fish-egg is lower than example 1, and its hatching rate is respectively: 62%, 53%; And control experiment group is not hatched successfully by the impact of unarmored dinoflagellate outburst.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (9)
1. cultivate the method that hailian seaweed controls unarmored dinoflagellate outburst in cabrilla nursery pond, it is characterized in that: comprise the steps,
1), after being scrubbed clean by cultivating pool, seawater is added;
2) use calcium hypochlorite to carry out pond water sterilization, after process 8 ~ 16h, use Sulfothiorine neutralizing treatment, and aeration 18 ~ 30h, add nutritive salt, hailian seaweed is inoculated in cultivating pool and cultivates; Preferably, hailian seaweed is the purebred hailian seaweed through laboratory culture; Preferably, the hailian seaweed cultivated in triangular flask is spread cultivation by bait bucket, be finally inoculated in cultivating pool and cultivate;
3) in cultivating pool, cultivate hailian seaweed after 4 ~ 8 days, Chi Shui is taupe, and hailian seaweed density reaches 3.5 ~ 50,000/ml, throws in cabrilla fish-egg; Preferably, the density of cabrilla fish-egg is 0.2 ~ 1.2 ten thousand/m
3; Preferably, 10,000/m
3.
2. cultivate the method that hailian seaweed controls unarmored dinoflagellate outburst in cabrilla nursery pond according to claim 1, it is characterized in that: step 2) in, the final concentration of chlorion is 18 ~ 22ppm; Add in Sulfothiorine and chlorion, wherein the final concentration of chlorion is 1:1 ~ 3:1 with the ratio of the final concentration of Sulfothiorine; Preferably, the final concentration of chlorion is 20ppm, and the final concentration of chlorion is 2:1 with the ratio of the final concentration of Sulfothiorine.
3. cultivate in cabrilla nursery pond according to claim 1 hailian seaweed control unarmored dinoflagellate outburst method, it is characterized in that: step 3) in, cultivate in cultivating pool hailian seaweed start to 22 ~ 26h during this period of time in, uninterrupted aeration.
4. cultivate the method that hailian seaweed controls unarmored dinoflagellate outburst in cabrilla nursery pond according to claim 1, it is characterized in that: step 2) in, described nutritive salt comprises ammonium nitrate, SODIUM PHOSPHATE, MONOBASIC, ironic citrate, water glass; After nutritive salt is joined seawater, the concentration of each component of ammonium nitrate, SODIUM PHOSPHATE, MONOBASIC, ironic citrate, water glass is respectively 6 ~ 14mg/L, 1 ~ 3mg/L, 0.05 ~ 0.35mg/L, 30 ~ 50mg/L.
5. cultivate the method that hailian seaweed controls unarmored dinoflagellate outburst in cabrilla nursery pond according to claim 1, it is characterized in that: step 2) in, described nutritive salt comprises ammonium nitrate, SODIUM PHOSPHATE, MONOBASIC, ironic citrate, water glass; After nutritive salt is joined seawater, the concentration of each component of ammonium nitrate, SODIUM PHOSPHATE, MONOBASIC, ironic citrate, water glass is respectively 8 ~ 12mg/L, 1.5 ~ 2.5mg/L, 0.1 ~ 0.35mg/L, 35 ~ 45mg/L.
6. cultivate the method that hailian seaweed controls unarmored dinoflagellate outburst in cabrilla nursery pond according to claim 1, it is characterized in that: step 2) in, described nutritive salt comprises ammonium nitrate, SODIUM PHOSPHATE, MONOBASIC, ironic citrate, water glass; After nutritive salt is joined seawater, the concentration of each component of ammonium nitrate, SODIUM PHOSPHATE, MONOBASIC, ironic citrate, water glass is respectively 10mg/L, 2mg/L, 0.2mg/L, 40mg/L.
7. in the cabrilla nursery pond according to any one of claim 4 ~ 6, cultivate the method that hailian seaweed controls unarmored dinoflagellate outburst, it is characterized in that: described nutritive salt is that the seawater by being heated to 55 ~ 65 DEG C fully dissolves, preferably, dissolve with the seawater of 60 DEG C.
8. in the cabrilla nursery pond according to any one of claim 1 ~ 7, cultivate the method that hailian seaweed controls unarmored dinoflagellate outburst, it is characterized in that: step 3) in, after throwing in cabrilla fish-egg, in cultivating pool, the density of hailian seaweed maintains 3.5 ~ 60,000 cell/mL.
9. in the cabrilla nursery pond according to any one of claim 1 ~ 7, cultivate the method that hailian seaweed controls unarmored dinoflagellate outburst, it is characterized in that: step 3) in, after throwing in cabrilla fish-egg, in cultivating pool, the density of hailian seaweed maintains 4 ~ 50,000 cell/mL.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106172093A (en) * | 2016-07-05 | 2016-12-07 | 武汉卓海青晨科技有限公司 | A kind of cultural method improving shrimp shoot survival percent |
| CN111183930A (en) * | 2020-02-25 | 2020-05-22 | 海南晨海水产有限公司 | Algae cultivation and water fertilization method for outdoor ecological breeding of grouper |
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Patent Citations (5)
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| CN106172093A (en) * | 2016-07-05 | 2016-12-07 | 武汉卓海青晨科技有限公司 | A kind of cultural method improving shrimp shoot survival percent |
| CN111183930A (en) * | 2020-02-25 | 2020-05-22 | 海南晨海水产有限公司 | Algae cultivation and water fertilization method for outdoor ecological breeding of grouper |
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