CN105130119A - Method for cultivating navicula tenera to control gymnodinium catenatum explosion in grouper fry growing pond - Google Patents

Method for cultivating navicula tenera to control gymnodinium catenatum explosion in grouper fry growing pond Download PDF

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Publication number
CN105130119A
CN105130119A CN201510543868.0A CN201510543868A CN105130119A CN 105130119 A CN105130119 A CN 105130119A CN 201510543868 A CN201510543868 A CN 201510543868A CN 105130119 A CN105130119 A CN 105130119A
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China
Prior art keywords
boat
cabrilla
shaped algae
cultivate
outburst
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CN201510543868.0A
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Chinese (zh)
Inventor
王妍
孙朦朦
王永苓
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Tianjin Ocean Pal Carol Biotech Co Ltd
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Tianjin Ocean Pal Carol Biotech Co Ltd
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Priority to CN201510543868.0A priority Critical patent/CN105130119A/en
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Abstract

The invention provides a method for cultivating navicula tenera to control gymnodinium catenatum explosion in a grouper fry growing pond. The method includes the following steps that (1) after the aquaculture pond is brushed clean, seawater is added; (2) calcium hypochlorite is used for conducting pond water disinfection, sodium thiosulfate is used for neutralizing treatment after treatment is performed for 8-16 hours, aeration is carried out for 18-30 hours, nutritive salt is added when chlorine in water disappears completely, and the navicula tenera is expanded into the aquaculture pond to be cultivated step by step; (3) after the navicula tenera is cultivated for 4-8 days in the aquaculture pond, grouper roes are thrown. According to the important role of the navicula tenera in the fry growing process of aquatic animals, the purebred navicula tenera is cultivated in the indoor cement pond. The navicula tenera is used for controlling gymnodinium catenatum explosion in the grouper fry growing pond, so that cost is low, the operation method is simple and the technology can be mastered easily. The cultivating pond water is tawny and moderate in transparency, the ecological system can be kept stable for a long time, the fry growing survival rate is increased, economic benefits are obvious and broad application prospects are achieved.

Description

The method that boat-shaped algae controls unarmored dinoflagellate outburst is cultivated in a kind of cabrilla nursery pond
Technical field
The invention belongs to aquaculture field, especially relate in a kind of cabrilla nursery pond and cultivate the method that boat-shaped algae controls unarmored dinoflagellate outburst.
Background technology
Unarmored dinoflagellate is under the jurisdiction of Pyrrophyta, Dinophyceae, unarmored dinoflagellate section, Gymnodinium, and most kind moves in seawater.Its individuality is small, nutritious, breeding is rapid, in the process of its growth and breeding, produce multiple toxin by own metabolism, and, water body color can be changed during unarmored dinoflagellate amount reproduction, larger harm is caused to aquatic animal.
Boat-shaped algae is as the simplest lower plant, protein, fat, carbohydrate, the necessary lipid acid of human body and vitamins and other nutritious components are rich in, as shellfish, prawn larvae and the part fish young direct open-mouthed bait and cultivate the nutritional fortification food of the secondary food organism such as wheel animalcule, halogen worm, copepods, can not be substituted in the status of aquatic fry growing.
At present in cabrilla nursery pond, usually there will be the prolific situation of unarmored dinoflagellate, have a strong impact on the water quality in nursery pond, the cultivation of cabrilla is caused to having a strong impact on.
Summary of the invention
In view of this, the present invention is intended to propose to cultivate the method that boat-shaped algae controls unarmored dinoflagellate outburst in a kind of cabrilla nursery pond, the method cost is low, working method simple and technology is easy to grasp, and while effectively avoiding the outburst of unarmored dinoflagellate in aquaculture water, improves survival rate of seedling.
For achieving the above object, technical scheme of the present invention is achieved in that
Cultivate the method that boat-shaped algae controls unarmored dinoflagellate outburst in cabrilla nursery pond, comprise the steps,
1), after being scrubbed clean by cultivating pool, seawater is added;
2) use calcium hypochlorite to carry out pond water sterilization, after process 8 ~ 16h, use Sulfothiorine neutralizing treatment, and aeration 18 ~ 30h, add nutritive salt, boat-shaped algae is inoculated in cultivating pool and cultivates; Preferably, boat-shaped algae is the purebred boat-shaped algae through laboratory culture; Preferably, the boat-shaped algae cultivated in triangular flask is spread cultivation by bait bucket, is finally inoculated in cultivating pool and cultivates;
3) in cultivating pool, cultivate boat-shaped algae after 4 ~ 8 days, Chi Shui is taupe, and boat-shaped algae density reaches 4 ~ 60,000/ml, throws in cabrilla fish-egg 0.5 ~ 1.5 ten thousand/m 3; Preferably, boat-shaped algae density reaches 50,000/ml, throws in cabrilla fish-egg 10,000/m 3.
Preferably, step 2) in, the final concentration of chlorion is 18 ~ 22ppm; Add in Sulfothiorine and chlorion, wherein the final concentration of chlorion is 1:1 ~ 3:1 with the ratio of the final concentration of Sulfothiorine; Preferably, the final concentration of chlorion is 20ppm; The final concentration of chlorion is 2:1 with the ratio of the final concentration of Sulfothiorine.
Preferably, step 3) in, cultivate in cultivating pool boat-shaped algae start to 22 ~ 26h during this period of time in, uninterrupted aeration, prevents frond from sinking.
Preferably, step 2) in, described nutritive salt comprises saltpetre, SODIUM PHOSPHATE, MONOBASIC, ironic citrate, water glass; After nutritive salt is joined seawater, the concentration of each component of saltpetre, SODIUM PHOSPHATE, MONOBASIC, ironic citrate, water glass is respectively 400 ~ 600mg/L, 40 ~ 60mg/L, 5 ~ 15mg/L, 500 ~ 700mg/L.。
Preferably, step 2) in, described nutritive salt comprises saltpetre, SODIUM PHOSPHATE, MONOBASIC, ironic citrate, water glass; After nutritive salt is joined seawater, the concentration of each component of saltpetre, SODIUM PHOSPHATE, MONOBASIC, ironic citrate, water glass is respectively 450 ~ 550mg/L, 45 ~ 55mg/L, 7 ~ 13mg/L, 550 ~ 650mg/L.
Preferably, step 2) in, described nutritive salt comprises saltpetre, SODIUM PHOSPHATE, MONOBASIC, ironic citrate, water glass; After nutritive salt is joined seawater, the concentration of each component of saltpetre, SODIUM PHOSPHATE, MONOBASIC, ironic citrate, water glass is respectively 500mg/L, 50mg/L, 10mg/L, 600mg/L.
Preferably, described nutritive salt is that the seawater by being heated to 55 ~ 65 DEG C fully dissolves, and preferably, dissolves with the seawater of 60 DEG C.
Preferably, step 3) in, after throwing in cabrilla fish-egg, in cultivating pool, the density of boat-shaped algae maintains 3 ~ 60,000 cell/mL.
Preferably, step 3) in, after throwing in cabrilla fish-egg, in cultivating pool, the density of boat-shaped algae maintains 4 ~ 50,000 cell/mL.
Relative to prior art, the method that boat-shaped algae controls unarmored dinoflagellate outburst is cultivated in cabrilla nursery pond of the present invention, there is following advantage: according to the vital role of boat-shaped algae in aquatic product animal raise seedling process, cultivate more purebred boat-shaped algae at indoor water mud sump.Utilize boat-shaped algae to control the outburst of unarmored dinoflagellate in described cabrilla nursery pond, cost is low, working method is simple and technology is easy to grasp; Culturing pool tawny, transparency is moderate, and the ecosystem can keep stable for a long time, improves survival rate of seedling, remarkable in economical benefits, has broad application prospects.
Embodiment
The present invention will be described in detail below by embodiment.
Embodiment one
For the floorage 25m of the cultivating pool of cabrilla nursery 2, the dark 2m in pond, adds seawater after being scrubbed clean by cultivating pool, and the degree of depth is 1.5m.Use calcium hypochlorite (use final concentration is 20ppm) to carry out pond water sterilization, (use final concentration is 8g/cm to use Sulfothiorine after process 12h 3) neutralizing treatment also continuous sudden and violent gas 24h.Treat that in water, chlorine all disappears, add nutritive salt, nutritive salt uses the seawater being heated to 60 DEG C fully to dissolve, now in cultivating pool, the concentration of saltpetre, SODIUM PHOSPHATE, MONOBASIC, ironic citrate, each component of water glass is respectively 500mg/L, 50mg/L, 10mg/L, 600mg/L, the boat-shaped algae cultivated in triangular flask is spread cultivation by bait bucket, is finally inoculated in cultivating pool and cultivates.In culturing process, uninterruptedly inflation in 24 hours, prevents frond from sinking; In cultivating pool, cultivate hailian seaweed after 5 days, then throw in cabrilla fish-egg, the boat-shaped algae controlling unarmored dinoflagellate outburst maintains 4.5 ten thousand cell/ml in the density in nursery pond.
Embodiment two
For the floorage 25m of the cultivating pool of cabrilla nursery 2, the dark 2m in pond, adds seawater after being scrubbed clean by cultivating pool, and the degree of depth is 1.6m.Use calcium hypochlorite (use final concentration is 20ppm) to carry out pond water sterilization, (use final concentration is 8g/cm to use Sulfothiorine after process 10h 3) neutralizing treatment also continuous sudden and violent gas 22h.Treat that in water, chlorine all disappears, add nutritive salt, nutritive salt uses the seawater being heated to 60 DEG C fully to dissolve, now in cultivating pool, the concentration of saltpetre, SODIUM PHOSPHATE, MONOBASIC, ironic citrate, each component of water glass is respectively 460mg/L, 48mg/L, 8mg/L, 560mg/L, the boat-shaped algae cultivated in triangular flask is spread cultivation by bait bucket, is finally inoculated in cultivating pool and cultivates.In culturing process, uninterruptedly inflation in 22 hours, prevents frond from sinking; In cultivating pool, cultivate hailian seaweed after 7 days, then throw in cabrilla fish-egg, the boat-shaped algae controlling unarmored dinoflagellate outburst maintains 4.1 ~ 4.2 ten thousand cell/ml in the density in nursery pond.
Embodiment three
For the floorage 25m of the cultivating pool of cabrilla nursery 2, the dark 2m in pond, adds seawater after being scrubbed clean by cultivating pool, and the degree of depth is 1.5m.Use calcium hypochlorite (use final concentration is 20ppm) to carry out pond water sterilization, (use final concentration is 8g/cm to use Sulfothiorine after process 12h 3) neutralizing treatment also continuous sudden and violent gas 28h.Treat that in water, chlorine all disappears, add nutritive salt, nutritive salt uses the seawater being heated to 60 DEG C fully to dissolve, now in cultivating pool, the concentration of saltpetre, SODIUM PHOSPHATE, MONOBASIC, ironic citrate, each component of water glass is respectively 540mg/L, 53mg/L, 12mg/L, 640mg/L, the boat-shaped algae cultivated in triangular flask is spread cultivation by bait bucket, is finally inoculated in cultivating pool and cultivates.In culturing process, uninterruptedly inflation in 24 hours, prevents frond from sinking; In cultivating pool, cultivate hailian seaweed after 7 days, then throw in cabrilla fish-egg, the boat-shaped algae controlling unarmored dinoflagellate outburst maintains 4.3 ~ 4.4 ten thousand cell/ml in the density in nursery pond.
Contrast experiment, for the area 25m of the cultivating pool of cabrilla nursery 2, the dark 2m in pond, adds seawater after being scrubbed clean by cultivating pool, and the degree of depth is 1.6m, throws in cabrilla fish-egg.
Detect the density of the unarmored dinoflagellate of embodiment one ~ embodiment three and contrast experiment, as shown in the table:
First day 5th day Tenth day 15 day Ahau
Embodiment one 0 cell/ml 0 cell/ml 33 cell/ml 33 cell/ml 33 cell/ml
Embodiment two 0 cell/ml 33 cell/ml 66 cell/ml 133 cell/ml 133 cell/ml
Embodiment three 0 cell/ml 0 cell/ml 33 cell/ml 33 cell/ml 33 cell/ml
Contrast experiment 0 cell/ml 166 cell/ml 1300 cell/ml 5800 cell/ml 1000 cell/ml
Meanwhile, observe the hatching situation of cabrilla fish-egg, result shows: in example 1,3, cabrilla fish-egg can normal incubation; In example 2, the hatching rate (hatching rate is 58%) of cabrilla fish-egg is lower than example 1 (hatching rate is 82%) and example 3 (hatching rate is 78%); And control experiment group is not hatched successfully by the impact of unarmored dinoflagellate outburst.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.

Claims (9)

1. cultivate the method that boat-shaped algae controls unarmored dinoflagellate outburst in cabrilla nursery pond, it is characterized in that: comprise the steps,
1), after being scrubbed clean by cultivating pool, seawater is added;
2) use calcium hypochlorite to carry out pond water sterilization, after process 8 ~ 16h, use Sulfothiorine neutralizing treatment, and aeration 18 ~ 30h, add nutritive salt, boat-shaped algae is inoculated in cultivating pool and cultivates; Preferably, boat-shaped algae is the purebred boat-shaped algae through laboratory culture; Preferably, the boat-shaped algae cultivated in triangular flask is spread cultivation by bait bucket, is finally inoculated in cultivating pool and cultivates;
3) in cultivating pool, cultivate boat-shaped algae after 4 ~ 8 days, Chi Shui is taupe, and boat-shaped algae density reaches 4 ~ 60,000/ml, throws in cabrilla fish-egg 0.5 ~ 1.5 ten thousand/m 3; Preferably, boat-shaped algae density reaches 50,000/ml, throws in cabrilla fish-egg 10,000/m 3.
2. cultivate the method that boat-shaped algae controls unarmored dinoflagellate outburst in cabrilla nursery pond according to claim 1, it is characterized in that: step 2) in, the final concentration of chlorion is 18 ~ 22ppm; Add in Sulfothiorine and chlorion, wherein the final concentration of chlorion is 1:1 ~ 3:1 with the ratio of the final concentration of Sulfothiorine; Preferably, the final concentration of chlorion is 20ppm; The final concentration of chlorion is 2:1 with the ratio of the final concentration of Sulfothiorine.
3. cultivate in cabrilla nursery pond according to claim 1 boat-shaped algae control unarmored dinoflagellate outburst method, it is characterized in that: step 3) in, cultivate in cultivating pool boat-shaped algae start to 22 ~ 26h during this period of time in, uninterrupted aeration.
4. cultivate the method that boat-shaped algae controls unarmored dinoflagellate outburst in cabrilla nursery pond according to claim 1, it is characterized in that: step 2) in, described nutritive salt comprises saltpetre, SODIUM PHOSPHATE, MONOBASIC, ironic citrate, water glass; After nutritive salt is joined seawater, the concentration of each component of saltpetre, SODIUM PHOSPHATE, MONOBASIC, ironic citrate, water glass is respectively 400 ~ 600mg/L, 40 ~ 60mg/L, 5 ~ 15mg/L, 500 ~ 700mg/L.。
5. cultivate the method that boat-shaped algae controls unarmored dinoflagellate outburst in cabrilla nursery pond according to claim 1, it is characterized in that: step 2) in, described nutritive salt comprises saltpetre, SODIUM PHOSPHATE, MONOBASIC, ironic citrate, water glass; After nutritive salt is joined seawater, the concentration of each component of saltpetre, SODIUM PHOSPHATE, MONOBASIC, ironic citrate, water glass is respectively 450 ~ 550mg/L, 45 ~ 55mg/L, 7 ~ 13mg/L, 550 ~ 650mg/L.
6. cultivate the method that boat-shaped algae controls unarmored dinoflagellate outburst in cabrilla nursery pond according to claim 1, it is characterized in that: step 2) in, described nutritive salt comprises saltpetre, SODIUM PHOSPHATE, MONOBASIC, ironic citrate, water glass; After nutritive salt is joined seawater, the concentration of each component of saltpetre, SODIUM PHOSPHATE, MONOBASIC, ironic citrate, water glass is respectively 500mg/L, 50mg/L, 10mg/L, 600mg/L.
7. according to the method for cultivating the outburst of boat-shaped algae control unarmored dinoflagellate in the cabrilla nursery pond described in claim 4 ~ 6, it is characterized in that: described nutritive salt is that the seawater by being heated to 55 ~ 65 DEG C fully dissolves, preferably, dissolving with the seawater of 60 DEG C.
8. in the cabrilla nursery pond according to any one of claim 1 ~ 7, cultivate the method that boat-shaped algae controls unarmored dinoflagellate outburst, it is characterized in that: step 3) in, after throwing in cabrilla fish-egg, in cultivating pool, the density of boat-shaped algae maintains 3 ~ 60,000 cell/mL.
9. in the cabrilla nursery pond according to any one of claim 1 ~ 7, cultivate the method that boat-shaped algae controls unarmored dinoflagellate outburst, it is characterized in that: step 3) in, after throwing in cabrilla fish-egg, in cultivating pool, the density of boat-shaped algae maintains 4 ~ 50,000 cell/mL.
CN201510543868.0A 2015-08-28 2015-08-28 Method for cultivating navicula tenera to control gymnodinium catenatum explosion in grouper fry growing pond Pending CN105130119A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106172093A (en) * 2016-07-05 2016-12-07 武汉卓海青晨科技有限公司 A kind of cultural method improving shrimp shoot survival percent

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CN102618444A (en) * 2011-04-13 2012-08-01 福建省水产研究所 Culture solution for directionally culturing young abalone feed such as Cocconeis
KR20130134476A (en) * 2012-05-31 2013-12-10 김해용 A farming method for fish fortified with vanadium
CN103444603A (en) * 2013-08-21 2013-12-18 中山大学 Method for culturing grouper fries in small water body

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Publication number Priority date Publication date Assignee Title
JP2001061466A (en) * 2000-07-31 2001-03-13 Higashimaru Shoyu Co Ltd Production of astaxanthin
CN1710062A (en) * 2004-06-18 2005-12-21 江苏省药用植物生物技术重点实验室 Scaphoid algae open culture method and its special culture medium
CN101675732A (en) * 2008-09-19 2010-03-24 中国水产科学研究院黄海水产研究所 Ecological treatment and seedling attaching method of shellfish attaching bases
CN101647406A (en) * 2009-09-04 2010-02-17 福建蓝鲸水产有限公司 Benthic diatom culture method for growing seedlings and abalone fry culture method
CN101836616A (en) * 2010-06-17 2010-09-22 广西红榄科技有限公司 Pollution-free healthy culture method of grouper in pond
CN101926298A (en) * 2010-07-29 2010-12-29 广东海洋大学 Method for constructing specific pathogen-free penaeid shrimp culture system
CN102618444A (en) * 2011-04-13 2012-08-01 福建省水产研究所 Culture solution for directionally culturing young abalone feed such as Cocconeis
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CN103444603A (en) * 2013-08-21 2013-12-18 中山大学 Method for culturing grouper fries in small water body

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106172093A (en) * 2016-07-05 2016-12-07 武汉卓海青晨科技有限公司 A kind of cultural method improving shrimp shoot survival percent

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Application publication date: 20151209