CN105232352B - A kind of polypeptide complex that wrinkle is gone for eye and preparation method thereof - Google Patents
A kind of polypeptide complex that wrinkle is gone for eye and preparation method thereof Download PDFInfo
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- CN105232352B CN105232352B CN201510733991.9A CN201510733991A CN105232352B CN 105232352 B CN105232352 B CN 105232352B CN 201510733991 A CN201510733991 A CN 201510733991A CN 105232352 B CN105232352 B CN 105232352B
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- polypeptide complex
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- 230000037303 wrinkles Effects 0.000 title claims abstract description 29
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 16
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 16
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 16
- 238000002360 preparation method Methods 0.000 title claims abstract description 7
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 20
- 229920002385 Sodium hyaluronate Polymers 0.000 claims abstract description 13
- 150000001875 compounds Chemical class 0.000 claims abstract description 13
- 235000011187 glycerol Nutrition 0.000 claims abstract description 13
- 229940010747 sodium hyaluronate Drugs 0.000 claims abstract description 13
- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 claims abstract description 13
- WSGCRSMLXFHGRM-DEVHWETNSA-N (2s)-2-[[(2s)-6-amino-2-[[(2s,3r)-2-[[(2s,3r)-2-[[(2s)-6-amino-2-(hexadecanoylamino)hexanoyl]amino]-3-hydroxybutanoyl]amino]-3-hydroxybutanoyl]amino]hexanoyl]amino]-3-hydroxypropanoic acid Chemical group CCCCCCCCCCCCCCCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(O)=O WSGCRSMLXFHGRM-DEVHWETNSA-N 0.000 claims abstract description 12
- 108010016626 Dipeptides Proteins 0.000 claims abstract description 12
- 125000001312 palmitoyl group Chemical group O=C([*])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 claims abstract description 12
- RJZNPROJTJSYLC-LLINQDLYSA-N (4s)-4-acetamido-5-[[(2s)-1-[[(2s)-1-[[(2s)-5-amino-1-[[(2s)-1-[[(2s)-1-amino-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-1,5-dioxopentan-2-yl]amino]-4-methylsulfanyl-1-oxobutan-2-yl]amino]-4-car Chemical compound OC(=O)CC[C@H](NC(C)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(N)=O RJZNPROJTJSYLC-LLINQDLYSA-N 0.000 claims abstract description 8
- 125000000218 acetic acid group Chemical group C(C)(=O)* 0.000 claims abstract description 4
- 238000003756 stirring Methods 0.000 claims description 40
- 239000000047 product Substances 0.000 claims description 13
- FHKSXSQHXQEMOK-UHFFFAOYSA-N hexane-1,2-diol Chemical class CCCCC(O)CO FHKSXSQHXQEMOK-UHFFFAOYSA-N 0.000 claims description 11
- ROTFCACGLKOUGI-JYJNAYRXSA-N (2s)-2-[[(2s)-2-[[(2s)-2-(3-acetamidopropanoylamino)-3-(1h-imidazol-5-yl)propanoyl]amino]-3-hydroxypropanoyl]amino]-3-(1h-imidazol-5-yl)propanoic acid Chemical compound C([C@H](NC(=O)CCNC(=O)C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC=1NC=NC=1)C(O)=O)C1=CN=CN1 ROTFCACGLKOUGI-JYJNAYRXSA-N 0.000 claims description 9
- 239000004475 Arginine Substances 0.000 claims description 8
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims description 8
- 239000000706 filtrate Substances 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 8
- 238000005374 membrane filtration Methods 0.000 claims description 8
- 239000011259 mixed solution Substances 0.000 claims description 8
- -1 palmityl tetrapeptide -7 Chemical compound 0.000 claims description 8
- AJLNZWYOJAWBCR-OOPVGHQCSA-N (4s)-4-acetamido-5-[[(2s)-1-[[(2s)-1-[[(2s)-5-amino-1-[[(2s)-1-[[(2s)-1-amino-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-1,5-dioxopentan-2-yl]amino]-4-methylsulfanyl-1-oxobutan-2-yl]amino]-4-car Chemical compound OC(=O)CC[C@H](NC(C)=O)C(=C)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CCCN=C(N)N)C(N)=O AJLNZWYOJAWBCR-OOPVGHQCSA-N 0.000 claims description 4
- 238000010438 heat treatment Methods 0.000 claims description 2
- DWNBOPVKNPVNQG-LURJTMIESA-N (2s)-4-hydroxy-2-(propylamino)butanoic acid Chemical compound CCCN[C@H](C(O)=O)CCO DWNBOPVKNPVNQG-LURJTMIESA-N 0.000 claims 1
- 125000002252 acyl group Chemical group 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 10
- 230000001153 anti-wrinkle effect Effects 0.000 abstract description 6
- 125000000913 palmityl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 abstract description 3
- 238000010612 desalination reaction Methods 0.000 abstract description 2
- 230000006872 improvement Effects 0.000 abstract description 2
- XXMIOPMDWAUFGU-UHFFFAOYSA-N hexane-1,6-diol Chemical class OCCCCCCO XXMIOPMDWAUFGU-UHFFFAOYSA-N 0.000 abstract 1
- 206010040954 Skin wrinkling Diseases 0.000 description 27
- 230000000052 comparative effect Effects 0.000 description 13
- 230000007423 decrease Effects 0.000 description 13
- 238000002156 mixing Methods 0.000 description 8
- 102000008186 Collagen Human genes 0.000 description 3
- 108010035532 Collagen Proteins 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 229920001436 collagen Polymers 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 230000004060 metabolic process Effects 0.000 description 3
- 241000282414 Homo sapiens Species 0.000 description 2
- 102000004183 Synaptosomal-Associated Protein 25 Human genes 0.000 description 2
- 108010057722 Synaptosomal-Associated Protein 25 Proteins 0.000 description 2
- 230000003712 anti-aging effect Effects 0.000 description 2
- 230000017531 blood circulation Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 230000004089 microcirculation Effects 0.000 description 2
- 230000008326 skin blood flow Effects 0.000 description 2
- 230000037331 wrinkle reduction Effects 0.000 description 2
- 229920000832 Cutin Polymers 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000007596 consolidation process Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000001815 facial effect Effects 0.000 description 1
- 238000009499 grossing Methods 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 230000004118 muscle contraction Effects 0.000 description 1
- 210000000715 neuromuscular junction Anatomy 0.000 description 1
- 239000002858 neurotransmitter agent Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000007665 sagging Methods 0.000 description 1
- 210000001732 sebaceous gland Anatomy 0.000 description 1
- 230000037075 skin appearance Effects 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 210000000106 sweat gland Anatomy 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
- A61K2800/522—Antioxidants; Radical scavengers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Dermatology (AREA)
- Emergency Medicine (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Cosmetics (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
It is by mass percentage, composed of the following components the invention provides a kind of polypeptide complex that wrinkle is gone for eye：0.02% 0.035% acetyl hexapeptide 3,0.001% 0.01% Matrixyl 3,0.025% 0.035% palmityl tetrapeptide 7,0.025% 0.035% palmitoyl tripeptide 3,0.08% 0.12% dipeptides 2,0.1% 0.2% acetyl tetrapeptide 5,4% 6% glycerine, 0.2% 0.4% Sodium Hyaluronate, 0.3% 0.5%1,2 hexylene glycols, surplus are pure water.Accordingly, the present invention also provides the preparation method of the compound.After a kind of polypeptide complex for eye anti-wrinkle provided by the invention, volunteer has the effect of obvious desalination eye wrinkle-removing, the number of the average wrinkle of eye is reduced, eye wrinkle-removing depth shallower, the smoothness of eye part skin improves, the area coverage of eye wrinkle-removing declines, and eye wrinkle-removing phenomenon has clear improvement.
Description
Technical field
The present invention relates to a kind of polypeptide complex that wrinkle is gone for eye and preparation method thereof, the compound is mainly used in eye
Portion's skin, there is wrinkle reduction, the effect of anti-aging.
Background technology
Eye circumference skin is the thickness at position, only skin of face a quarter most thin in all skin of human body, is not almost had
There is the distribution of sebaceous glands and sweat gland, thus preserve and be not easy without natural moist ability, moisture, easily cause dry water shortage, lead
Cause the easily aging of eye circumference skin.With advancing age, easily there is wrinkle, expression line, crow's feet in eye skin, follows the string
It is sagging with consolidation, relaxation.
Currently used anti-wrinkle product is various, and effect and effect are variant greatly, and most of product can not generally improve skin
Metabolism, promote skin blood circulation, increase skin-nourishing, can not fundamentally remove the wrinkle and color spot of skin appearance.It is and more
Number products contain heavy metal, security and sequelae is still difficult predicts, if improper use, may after cause the damage of skin.
The content of the invention
For above-mentioned technical problem, the invention provides a kind of polypeptide complex that wrinkle is gone for eye, using small molecule
Polypeptide, molecular weight are small, it is not necessary to and the auxiliary of other penetration-assisting agents directly can independently penetrate to the skin deep layer, dredge capillary,
Blood viscosity is reduced, accelerates VPV, improves facial microcirculation, cutin thickness is reduced after promoting skin of face metabolism
Degree, cell is obtained the nutrient of abundance, improve the self-repairing capability of cell, fundamentally improve skin, dispel, desalinate eye
Wrinkle, accordingly, present invention also offers the preparation method of the compound.
In order to realize the technical purpose of the present invention, the present invention uses following technical scheme.
A kind of polypeptide complex that wrinkle is gone for eye, it is by mass percentage, composed of the following components：0.02%-
0.035% acetyl hexapeptide -3,0.001%-0.01% Matrixyl -3,0.025%-0.035% palmityl tetrapeptide -
7th, 0.025%-0.035% palmitoyl tripeptide 3,0.08%-0.12% dipeptides -2,0.1%-0.2% acetyl tetrapeptide -5,4%-
6% glycerine, 0.2%-0.4% Sodium Hyaluronate, 0.3%-0.5% 1,2- hexylene glycols, surplus is pure water.
Accordingly, present invention also offers the preparation method of the compound, comprise the following steps：
(a) 70%-80% pure water is added by proportioning, heating, adds 1,2- hexylene glycols, after stirring dissolves it, etc.
It is divided into two parts, respectively A phases, B phases；
(b) it is 50 DEG C -80 DEG C to keep A phases temperature, and stirring adds Sodium Hyaluronate, and continuing stirring makes its dissolving mixing equal
It is even, glycerine is added, continues to be uniformly mixed, as I phase；
(c) acetyl hexapeptide -3, Matrixyl -3, palmityl tetrapeptide -7, palmitoyl tripeptide 3, acetyl tetrapeptide -5, dipeptides -2 are added
Enter into B phases, after stirring and dissolving is well mixed, with membrane filtration, gained filtrate is II phase；
(d) II phase is added into I phase under stirring, the appearance of II phase is rinsed respectively with the pure water of remaining proportional quantity
Device, and rinse liquid is added in I phase, it is uniformly mixed；
(e) it is 6.0-7.0 to adjust mixed solution pH value with arginine, and gained compound is product of the present invention.
Preferably, heating-up temperature is 80 DEG C in the step (a).
Preferably, the filter hole of filter membrane is 0.45 μm in the step (c).
Preferably, arginic concentration is 15wt% in the step (e).
A kind of polypeptide complex for eye anti-wrinkle provided by the invention, according to the characteristics of eye skin and wrinkle shape
Into mechanism, suppress Angiotensin-Converting activity by adding acetyl tetrapeptide -5, relaxation capillary tension force to promote blood flow,
Accelerate eye skin blood circulation, there is antioedematous function；Dipeptides -2 are added, blood circulation can be strengthened, accelerate VPV,
Promote metabolism；Acetyl hexapeptide -3 are added, imitate SNAP-25 protein N terminals, competition SNAP-25 is participated in and is melting bubble complex
Site, so as to regulator configuration, and melt bubble complex it is slightly unstable, vesica can not effective release neurotransmitters,
Contraction of muscle is caused to weaken, it is therefore prevented that the formation of wrinkle；Palmitoyl tripeptide 3 smoothing wrinkle is added, neuromuscular junction acetyl courage can be suppressed
The release of alkali, muscle tone is set to decline or benumb, wrinkle also fades away therewith, so as to reach the purpose of wrinkle reduction；Add
Matrixyl -3 promotes collagen generation, prevents from generating wrinkle because collagen reduces；And add palmityl tetrapeptide -7
With the effect for reducing inflammation；Sodium Hyaluronate and glycerine are a kind of NMFs；1,2- hexylene glycol has the function that anti-bacteria wet keeping,
Improve the holding time of blending ingredients.
The invention has the advantages that：
(1) micromolecule polypeptide added independently can directly penetrate to the skin skin corium, be easily absorbed by the skin, can solve the problem that
Due to the eye wrinkle-removing caused by skin caused by blood is not smooth crosses presenility the problems such as, go the effect of wrinkle very good, while can
To improve face blood microcirculation, promote skin collagen generation, have the function that to improve skin quality.
(2) Sodium Hyaluronate and glycerine are added as the oxidation resistant raw material of skin-moisturizing, skin, microgroove is removed with preferably crease-resistant
Effect.
(3) technological process of production is simple.
Embodiment
In order to be better understood from the present invention, invention is described in detail with reference to embodiment, unless otherwise instructed, with
Lower embodiment is by percentage to the quality.
Embodiment 1：
(a) 70% pure water is added by proportioning, is heated to 80 DEG C, adds 0.3% 1,2- hexylene glycols, stirring makes its molten
Xie Hou, it is divided into two parts, respectively A phases, B phases；
(b) it is 50 DEG C to keep A phases temperature, and stirring adds 0.4% Sodium Hyaluronate, and continuing stirring makes its dissolving mixing equal
It is even, 4% glycerine is added, continues to be uniformly mixed, as I phase；
(c) by the Matrixyl -3 of 0.02% acetyl hexapeptide -3,0.01%, 0.025% palmityl tetrapeptide -7,
0.035% palmitoyl tripeptide 3, the dipeptides -2 of 0.1% acetyl tetrapeptide -5 and 0.08% are added in B phases, and stirring and dissolving is well mixed
Afterwards, with the membrane filtration that filter hole is 0.45 μm, gained filtrate is II phase；
(d) II phase is added into I phase under stirring, the appearance of II phase is rinsed respectively with the pure water of remaining proportional quantity
Device, and rinse liquid is added in I phase, it is uniformly mixed；
(e) it is 6.0-7.0 to adjust mixed solution pH value with 15wt% arginine, and gained compound is the present invention
The product of embodiment 1.
Embodiment 2：
(a) 80% pure water is added by proportioning, is heated to 80 DEG C, adds 0.5% 1,2- hexylene glycols, stirring makes its molten
Xie Hou, it is divided into two parts, respectively A phases, B phases；
(b) it is 60 DEG C to keep A phases temperature, and stirring adds 0.4% Sodium Hyaluronate, and continuing stirring makes its dissolving mixing equal
It is even, 5% glycerine is added, continues to be uniformly mixed, as I phase；
(c) by the Matrixyl -3 of 0.025% acetyl hexapeptide -3,0.007%, 0.035% palmityl tetrapeptide -7,
0.035% palmitoyl tripeptide 3, the dipeptides -2 of 0.1% acetyl tetrapeptide -5 and 0.1% are added in B phases, and stirring and dissolving is well mixed
Afterwards, with the membrane filtration that filter hole is 0.45 μm, gained filtrate is II phase；
(d) II phase is added into I phase under stirring, the appearance of II phase is rinsed respectively with the pure water of remaining proportional quantity
Device, and rinse liquid is added in I phase, it is uniformly mixed；
(e) it is 6.0-7.0 to adjust mixed solution pH value with 15wt% arginine, and gained compound is the present invention
The product of embodiment 2.
Embodiment 3：
(a) 80% pure water is added by proportioning, is heated to 80 DEG C, adds 0.4% 1,2- hexylene glycols, stirring makes its molten
Xie Hou, it is divided into two parts, respectively A phases, B phases；
(b) it is 70 DEG C to keep A phases temperature, and stirring adds 0.2% Sodium Hyaluronate, and continuing stirring makes its dissolving mixing equal
It is even, 6% glycerine is added, continues to be uniformly mixed, as I phase；
(c) by the Matrixyl -3 of 0.03% acetyl hexapeptide -3,0.001%, 0.03% palmityl tetrapeptide -7,
0.03% palmitoyl tripeptide 3, the dipeptides -2 of 0.15% acetyl tetrapeptide -5 and 0.12% are added in B phases, and stirring and dissolving is well mixed
Afterwards, with the membrane filtration that filter hole is 0.45 μm, gained filtrate is II phase；
(d) II phase is added into I phase under stirring, the appearance of II phase is rinsed respectively with the pure water of remaining proportional quantity
Device, and rinse liquid is added in I phase, it is uniformly mixed；
(e) it is 6.0-7.0 to adjust mixed solution pH value with 15wt% arginine, and gained compound is the present invention
The product of embodiment 3.
Embodiment 4：
(a) 75% pure water is added by proportioning, is heated to 80 DEG C, adds 0.5% 1,2- hexylene glycols, stirring makes its molten
Xie Hou, it is divided into two parts, respectively A phases, B phases；
(b) it is 80 DEG C to keep A phases temperature, and stirring adds 0.3% Sodium Hyaluronate, and continuing stirring makes its dissolving mixing equal
It is even, 6% glycerine is added, continues to be uniformly mixed, as I phase；
(c) by the Matrixyl -3 of 0.035% acetyl hexapeptide -3,0.005%, 0.032% palmityl tetrapeptide -7,
0.025% palmitoyl tripeptide 3, the dipeptides -2 of 0.2% acetyl tetrapeptide -5 and 0.12% are added in B phases, and stirring and dissolving is well mixed
Afterwards, with the membrane filtration that filter hole is 0.45 μm, gained filtrate is II phase；
(d) II phase is added into I phase under stirring, the appearance of II phase is rinsed respectively with the pure water of remaining proportional quantity
Device, and rinse liquid is added in I phase, it is uniformly mixed；
(e) it is 6.0-7.0 to adjust mixed solution pH value with 15wt% arginine, and gained compound is the present invention
The product of embodiment 4.
Comparative example 5 (does not contain acetyl hexapeptide -3)：
(a) 75% pure water is added by proportioning, is heated to 80 DEG C, adds 0.5% 1,2- hexylene glycols, stirring makes its molten
Xie Hou, it is divided into two parts, respectively A phases, B phases；
(b) it is 80 DEG C to keep A phases temperature, and stirring adds 0.3% Sodium Hyaluronate, and continuing stirring makes its dissolving mixing equal
It is even, 6% glycerine is added, continues to be uniformly mixed, as I phase；
(c) 0.005% Matrixyl -3, the palmitoyl tripeptide 3 of 0.032% palmityl tetrapeptide -7,0.025%, 0.2%
The dipeptides -2 of acetyl tetrapeptide -5 and 0.12% be added in B phases, after stirring and dissolving is well mixed, with the filter that filter hole is 0.45 μm
Membrane filtration, gained filtrate are II phase；
(d) II phase is added into I phase under stirring, the appearance of II phase is rinsed respectively with the pure water of remaining proportional quantity
Device, and rinse liquid is added in I phase, it is uniformly mixed；
(e) it is 6.0-7.0 to adjust mixed solution pH value with 15wt% arginine, and gained compound is the present invention
The product of comparative example 5.
Comparative example 6 (does not contain acetyl tetrapeptide -5)：
(a) 80% pure water is added by proportioning, is heated to 80 DEG C, adds 0.4% 1,2- hexylene glycols, stirring makes its molten
Xie Hou, it is divided into two parts, respectively A phases, B phases；
(b) it is 70 DEG C to keep A phases temperature, and stirring adds 0.2% Sodium Hyaluronate, and continuing stirring makes its dissolving mixing equal
It is even, 6% glycerine is added, continues to be uniformly mixed, as I phase；
(c) by the Matrixyl -3 of 0.03% acetyl hexapeptide -3,0.001%, 0.03% palmityl tetrapeptide -7,
0.03% palmitoyl tripeptide 3 and 0.12% dipeptides -2 are added in B phases, are 0.45 μm with filter hole after stirring and dissolving is well mixed
Membrane filtration, gained filtrate are II phase；
(d) II phase is added into I phase under stirring, the appearance of II phase is rinsed respectively with the pure water of remaining proportional quantity
Device, and rinse liquid is added in I phase, it is uniformly mixed；
(e) it is 6.0-7.0 to adjust mixed solution pH value with 15wt% arginine, and gained compound is the present invention
The product of comparative example 6.
The obvious healthy volunteer 180 of wrinkle is selected, average age is 50.4 years old, 6 groups is divided into, respectively to embodiment
1-4 products obtained therefroms and the product of comparative example 5, comparative example 6 are tested.Daily sooner or later compound described in circumference of eyes smearing
Thing, anti-aging effects detection pass through profile and graphical analysis, take pictures and compare.
28 days before and after embodiment 1-4 and comparative example 5,6 uses, 56 days using effects are respectively as shown in table 1, table 2.
The embodiment of table 1 and comparative example use the eye anti-wrinkle effect after 28 days
| Detection project | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Comparative example 5 | Comparative example 6 |
| Wrinkle number | Reduce 28.7% | Reduce 29.1% | Reduce 27.4% | Reduce 25.2% | Reduce 19.9% | Reduce 22.7% |
| Wrinkle depth | Shoal 19.8% | Shoal 21.7% | Shoal 18.2% | Shoal 16.4% | Shoal 13.7% | Shoal 15.2% |
| Eye part skin smoothness | Improve 30.8% | Improve 32.7% | Improve 31.2% | Improve 29.4% | Improve 20.9% | Improve 24.5% |
| Wrinkle area coverage | Decline 33.4% | Decline 34.5% | Decline 30.7% | Decline 32.2% | Decline 21.7% | Decline 25.6% |
The embodiment of table 2 and comparative example use the eye anti-wrinkle effect after 56 days
| Detection project | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Comparative example 5 | Comparative example 6 |
| Wrinkle number | Reduce 35.7% | Reduce 36.4% | Reduce 35.0% | Reduce 35.9% | Reduce 24.9% | Reduce 28.0% |
| Wrinkle depth | Shoal 26.4% | Shoal 27.8% | Shoal 25.9% | Shoal 26.3% | Shoal 20.8% | Shoal 22.4% |
| Eye part skin smoothness | Improve 43.2% | Improve 41.6% | Improve 41.2% | Improve 42.1% | Improve 24.7% | Improve 27.9% |
| Wrinkle area coverage | Decline 43.9% | Decline 44.3% | Decline 43.2% | Decline 42.7% | Decline 29.6% | Decline 31.1% |
According to upper table data, it is apparent that using a kind of polypeptide complex for eye anti-wrinkle provided by the invention
Afterwards, volunteer has the effect of obvious desalination eye wrinkle-removing, and the number of the average wrinkle of eye is reduced, and eye wrinkle-removing depth becomes
Shallow, the smoothness of eye part skin improves, and the area coverage of eye wrinkle-removing declines, and eye wrinkle-removing phenomenon has clear improvement.
The basic principles, principal features and advantages of the present invention have been shown and described above.The technical staff of the industry should
Understand, the invention is not limited in any way for above-described embodiment, all to be obtained by the way of equivalent substitution or equivalent transformation
Technical scheme, all fall within protection scope of the present invention.
Claims (4)
1. a kind of polypeptide complex that wrinkle is gone for eye, it is characterised in that by mass percentage, composed of the following components：
0.02%-0.035% acetyl hexapeptide -3,0.001%-0.01% Matrixyl -3,0.025%-0.035% palm
Acyl tetrapeptide -7,0.025%-0.035% palmitoyl tripeptide 3,0.08%-0.12% dipeptides -2,0.1%-0.2% acetyl tetrapeptide -
5th, 4%-6% glycerine, 0.2%-0.4% Sodium Hyaluronate, 0.3%-0.5% 1,2- hexylene glycols, surplus are pure water；
The preparation method of the polypeptide complex for going to wrinkle for eye comprises the following steps：
(a) 70%-80% pure water is added by proportioning, heating, 1,2- hexylene glycols is added, after stirring dissolves it, is divided into
Two parts, respectively A phases, B phases；
(b) it is 50 DEG C -80 DEG C to keep A phases temperature, and stirring adds Sodium Hyaluronate, and continuing stirring makes its dissolving well mixed, then
Glycerine is added, continues to be uniformly mixed, as I phase；
(c) acetyl hexapeptide -3, Matrixyl -3, palmityl tetrapeptide -7, palmitoyl tripeptide 3, acetyl tetrapeptide -5, dipeptides -2 are added to B
Xiang Zhong, after stirring and dissolving is well mixed, with membrane filtration, gained filtrate is II phase；
(d) II phase is added into I phase under stirring, the container of II phase is rinsed respectively with the pure water of remaining proportional quantity,
And rinse liquid is added in I phase, it is uniformly mixed；
(e) it is 6.0-7.0 to adjust mixed solution pH value with arginine, and gained compound is product of the present invention.
2. the polypeptide complex according to claim 1 that wrinkle is gone for eye, it is characterised in that：Add in the step (a)
Hot temperature is 80 DEG C.
3. the polypeptide complex according to claim 1 that wrinkle is gone for eye, it is characterised in that：Filtered in the step (c)
The filter opening of film is 0.45 μm.
4. the polypeptide complex according to claim 1 that wrinkle is gone for eye, it is characterised in that：It is smart in the step (e)
The concentration of propylhomoserin is 15wt%.
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| CN106344426A (en) * | 2016-10-08 | 2017-01-25 | 广州丹奇日用化工厂有限公司 | Retina containing new zealand spinach polypeptide |
| CN106726668A (en) * | 2016-11-19 | 2017-05-31 | 诺斯贝尔化妆品股份有限公司 | A kind of many peptidoliposome raw materials used in cosmetics |
| CN108338934B (en) * | 2017-01-23 | 2020-11-27 | 珠海联邦制药股份有限公司 | Polypeptide composition for eye care and application thereof |
| CN107308020B (en) * | 2017-07-06 | 2020-11-27 | 珠海联邦制药股份有限公司 | Stable polypeptide composition and application thereof |
| CN107550755B (en) * | 2017-10-27 | 2019-01-25 | 花安堂生物科技集团有限公司 | A kind of composition and preparation method thereof of mild moisturizing desalination microgroove |
| CN109745280A (en) * | 2017-11-01 | 2019-05-14 | 郑州艾之宝科技有限公司 | The net face epithelium of Chinese medicine |
| CN108078801A (en) * | 2017-12-22 | 2018-05-29 | 安婕妤化妆品科技股份有限公司 | A kind of eye cosmetic composition |
| CN114073662A (en) * | 2020-08-11 | 2022-02-22 | 广东长昊药业股份有限公司 | Intelligent care mother solution for eye and preparation method thereof |
| CN113116742A (en) * | 2021-04-29 | 2021-07-16 | 山东优杰生物科技有限公司 | Composite polypeptide anti-wrinkle composition and preparation method and application thereof |
| CN115624504A (en) * | 2022-03-08 | 2023-01-20 | 广州市万千粉丝化妆品有限公司 | Application of small molecular tissue active peptide in anti-wrinkle and anti-aging cosmetics for promoting skin collagen reconstruction and preparation method thereof |
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