CN105230606A - Vitrification unfreezing carrier - Google Patents
Vitrification unfreezing carrier Download PDFInfo
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- CN105230606A CN105230606A CN201510601903.XA CN201510601903A CN105230606A CN 105230606 A CN105230606 A CN 105230606A CN 201510601903 A CN201510601903 A CN 201510601903A CN 105230606 A CN105230606 A CN 105230606A
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- carrier
- box body
- circular trough
- drink
- spring
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Abstract
The invention discloses a vitrification unfreezing carrier, comprising a box body, a box cover, circular grooves, a square groove, an externally-inclining wedge-shaped groove and an internally-inclining wedge-shaped groove, wherein the circular grooves are arranged on the left end of the box body, the square groove is arranged on the right end of the box body, the circular grooves are fixedly connected with the square groove, the box body is provided with the externally-inclining wedge-shaped groove, and the box cover is provided with the internally-inclining wedge-shaped groove. The vitrification unfreezing carrier simplifies the unfreezing process of embryos, shortens the unfreezing time of the embryos, stabilized the osmotic pressure of a reagent and reduces probability of errors.
Description
Technical field
The present invention relates to technical field of medical instruments, be specifically related to a kind of vitrifying and thaw carrier.
Background technology
At present, embryo transfer technology has been widely used popularization, the frozen embryo used is stored in-196 DEG C of liquid nitrogen, must thaw before the use, according to the principle of frozen embryo, freezing biological tissue, the speed that quality after it is freezing is frozen with it has certain relation, namely freezing rate is faster, biological tissue is more similar to without freezing basic stitch on biochemistry with physiological indices, freezing speed is slower, the biochemistry of biological tissue and physical signs can change greatly, even can there is death in serious, living tissue cannot be preserved for a long time.Thaw embryo as freezing inverse process, and thaw routine follows mentioned above principle too, freezing biological tissue, thawing rate is faster, and its structural constituent can return to close to organizing freezing front state after thawing; Thawing Rate is slow, and the change that biological tissue occurs is then comparatively large, can not survive after likely thawing.Due in the glass freezing process of embryo, the mode of a snap frozen is used to be undertaken freezing by embryo, therefore its course of defrosting, be also the process that express delivery is thawed.And need in existing embryo thawing process frozen embryo to be placed in water temperature at 36-40 DEG C of hot water, and be placed on each and contain in the plane ware of different thawing solution, multiple plate need be used when thawing, repeatedly mark easily to produce and obscure.
Summary of the invention
The object of the invention is to solve in embryo thawing process, the reagent that thaws is more, uses multiple plate not easily to indicate, causes confusion, and provides a kind of vitrifying to thaw carrier, comprises box body, lid, circular trough, square groove, outer oblique ancient festival in spring and autumn held on water banks to drink and cleanse away the evil influences shape groove and interior oblique ancient festival in spring and autumn held on water banks to drink and cleanse away the evil influences shape groove; Described circular trough is arranged on box body left end, and described square groove is arranged on box body right-hand member, and circular trough is fixedly connected with square groove, and described box body arranges outer oblique ancient festival in spring and autumn held on water banks to drink and cleanse away the evil influences shape groove, and described lid arranges interior oblique ancient festival in spring and autumn held on water banks to drink and cleanse away the evil influences shape groove.
Preferably, described circular trough arranges 2-5, and described circular trough diameter is 10-15mm.
Further preferred, described circular trough is set to 3, and the number of the circular trough used is according to the used reagent and determining of thawing.
The described square groove length of side is 20-25mm.
Described outer oblique ancient festival in spring and autumn held on water banks to drink and cleanse away the evil influences shape groove is provided with the gear that can seal with lid.
The invention has the beneficial effects as follows:
Thaw on carrier and 2-5 circular trough is set, more reagent is used in embryo thawing process, overcome obscuring of using plane ware to thaw to cause and be difficult to the problem that marks, the present invention's carrier that thaws carries out the mark of reagent in the box body blank space that circular trough is corresponding, be convenient to identify, can not produce to obscure and reduce and slip up.
This carrier can at room temperature thaw, freezing embryo is enable to recover its biochemical as early as possible, its biochemical, physical signs can not change, avoid the complicated procedures thawed in the hot water, reduce the probability infecting outside bacterium, improve thaw conception rate and the implantation rate of frozen embryo.
Accompanying drawing explanation
Fig. 1 is that vitrifying of the present invention is thawed carrier structure schematic diagram.
Fig. 2 is that vitrifying of the present invention is thawed carrier right view.
Fig. 3 is that vitrifying of the present invention is thawed carrier vertical view.
Fig. 4 is that vitrifying of the present invention is thawed carrier box body schematic diagram.
Fig. 5 is that vitrifying of the present invention is thawed carrier box body right view.
Fig. 6 is that vitrifying of the present invention is thawed carrier lid schematic diagram.
Fig. 7 is that vitrifying of the present invention is thawed carrier lid right view.
In figure, 1-box body, 2-lid, 3-circular trough, 4-square groove, the outer tiltedly ancient festival in spring and autumn held on water banks to drink and cleanse away the evil influences shape groove of 5-, oblique ancient festival in spring and autumn held on water banks to drink and cleanse away the evil influences shape groove in 6-.
Embodiment
Embodiment 1
A kind of vitrifying is thawed carrier, comprises box body 1, lid 2, circular trough 3, square groove 4, outer oblique ancient festival in spring and autumn held on water banks to drink and cleanse away the evil influences shape groove 5 and interior oblique ancient festival in spring and autumn held on water banks to drink and cleanse away the evil influences shape groove 6; Box body 1 left end arranges 2-5 circular trough 3, circular trough diameter is 10-15mm, described square groove 4 is arranged on box body 1 right-hand member, the length of side is 20-25mm, circular trough 3 is fixedly connected with square groove 4, described box body 1 arranges outer oblique ancient festival in spring and autumn held on water banks to drink and cleanse away the evil influences shape groove 5, and outer oblique ancient festival in spring and autumn held on water banks to drink and cleanse away the evil influences shape groove 5 is provided with the gear that can seal with lid, and described lid 2 arranges interior oblique ancient festival in spring and autumn held on water banks to drink and cleanse away the evil influences shape groove 6.
Embodiment 2
During embryo thawing, straw is taken out from liquid nitrogen, stop in atmosphere after 5 seconds, by straw, the vitrifying moved to successively containing 3 kinds of different thawing solutions is thawed in the circular trough 3 of carrier, circular trough 3 carries out mark at the box body place of correspondence, finally embryo is placed in the square groove 4 containing culture fluid, covers lid 2, continue to observe embryonic development situation in culture fluid.
Claims (5)
1. vitrifying is thawed a carrier, it is characterized in that, comprises box body (1), lid (2), circular trough (3), square groove (4), outer oblique ancient festival in spring and autumn held on water banks to drink and cleanse away the evil influences shape groove (5) and interior oblique ancient festival in spring and autumn held on water banks to drink and cleanse away the evil influences shape groove (6); Described circular trough (3) is arranged on box body (1) left end, described square groove (4) is arranged on box body (1) right-hand member, circular trough (3) is fixedly connected with square groove (4), and described box body (1) arranges outer oblique ancient festival in spring and autumn held on water banks to drink and cleanse away the evil influences shape groove (5), and described lid (2) arranges interior oblique ancient festival in spring and autumn held on water banks to drink and cleanse away the evil influences shape groove (6).
2. vitrifying according to claim 1 is thawed carrier, it is characterized in that, described circular trough (3) arranges 2-5, and described circular trough diameter is 10-15mm.
3. vitrifying according to claim 1 is thawed carrier, and it is characterized in that, described circular trough (3) arranges 3.
4. vitrifying according to claim 1 is thawed carrier, and it is characterized in that, described square groove (4) length of side is 20-25mm.
5. vitrifying according to claim 1 is thawed carrier, and it is characterized in that, described outer oblique ancient festival in spring and autumn held on water banks to drink and cleanse away the evil influences shape groove (5) is provided with the gear that can seal with lid.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510601903.XA CN105230606A (en) | 2015-09-21 | 2015-09-21 | Vitrification unfreezing carrier |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510601903.XA CN105230606A (en) | 2015-09-21 | 2015-09-21 | Vitrification unfreezing carrier |
Publications (1)
Publication Number | Publication Date |
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CN105230606A true CN105230606A (en) | 2016-01-13 |
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CN201510601903.XA Pending CN105230606A (en) | 2015-09-21 | 2015-09-21 | Vitrification unfreezing carrier |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106900693A (en) * | 2017-01-11 | 2017-06-30 | 金华市人民医院 | Embryo vitrifying freezing process and corresponding embryo vitrifying defrosting ware |
WO2022161195A1 (en) * | 2021-02-01 | 2022-08-04 | 深圳拜尔洛克生物技术有限公司 | Thaw recovery system and method for biomaterial |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20080247915A1 (en) * | 2007-04-05 | 2008-10-09 | Genx International, Inc. | Apparatus for the preparation of mammalian specimens for cryopreservation and/or vitrification, and for thawing and using susch specimens |
CN201395598Y (en) * | 2009-03-12 | 2010-02-03 | 山东省医学科学院基础医学研究所 | Sealed type micro porous culture plate |
CN203728851U (en) * | 2014-03-18 | 2014-07-23 | 于艳 | Vitrification refrigeration and resurrection culture plate |
CN205040513U (en) * | 2015-09-21 | 2016-02-24 | 南宁市第二人民医院 | Glass dissolves and freezes carrier |
-
2015
- 2015-09-21 CN CN201510601903.XA patent/CN105230606A/en active Pending
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20080247915A1 (en) * | 2007-04-05 | 2008-10-09 | Genx International, Inc. | Apparatus for the preparation of mammalian specimens for cryopreservation and/or vitrification, and for thawing and using susch specimens |
CN201395598Y (en) * | 2009-03-12 | 2010-02-03 | 山东省医学科学院基础医学研究所 | Sealed type micro porous culture plate |
CN203728851U (en) * | 2014-03-18 | 2014-07-23 | 于艳 | Vitrification refrigeration and resurrection culture plate |
CN205040513U (en) * | 2015-09-21 | 2016-02-24 | 南宁市第二人民医院 | Glass dissolves and freezes carrier |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106900693A (en) * | 2017-01-11 | 2017-06-30 | 金华市人民医院 | Embryo vitrifying freezing process and corresponding embryo vitrifying defrosting ware |
CN106900693B (en) * | 2017-01-11 | 2022-05-27 | 金华市人民医院 | Embryo vitrification unfreezing method and corresponding embryo vitrification unfreezing dish |
WO2022161195A1 (en) * | 2021-02-01 | 2022-08-04 | 深圳拜尔洛克生物技术有限公司 | Thaw recovery system and method for biomaterial |
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Application publication date: 20160113 |