CN215560387U - Liquid suction assembly for culturing biological tissue - Google Patents

Liquid suction assembly for culturing biological tissue Download PDF

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Publication number
CN215560387U
CN215560387U CN202122133011.5U CN202122133011U CN215560387U CN 215560387 U CN215560387 U CN 215560387U CN 202122133011 U CN202122133011 U CN 202122133011U CN 215560387 U CN215560387 U CN 215560387U
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insertion rod
biological tissue
liquid suction
assembly
rod
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CN202122133011.5U
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严飞
余裕炉
侯国宾
戴甄
贾晓伟
林泽洪
王沛
佘中六
王宪华
郭二勇
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Chengdu Aiweifu Biotechnology Co ltd
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Chengdu Aiweifu Biotechnology Co ltd
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Abstract

The utility model discloses a liquid suction component for culturing biological tissues, and relates to the technical field of biological tissue culture; a wicking assembly for use in culturing biological tissue comprising: the lower end part of the insertion rod is used for inserting the liquid suction head, the insertion rod is provided with a suction channel used for connecting a negative pressure source, and the suction channel extends from the lower end surface of the insertion rod to the upper end of the insertion rod; the first linear driving mechanism is used for driving the insertion rod to move up and down; the pipe-releasing clamping jaw is in driving connection with the second linear driving mechanism and can move to the moving path of the insertion rod from the outer side of the insertion rod, so that the liquid suction head sleeved on the insertion rod is released relative to the pipe-releasing clamping jaw through the insertion rod. The utility model can be used for automatically absorbing the biological tissue culture solution and meets the requirement of batch culture of biological tissues.

Description

Liquid suction assembly for culturing biological tissue
Technical Field
The utility model relates to the technical field of biological tissue culture, in particular to a liquid suction assembly for culturing biological tissue.
Background
In Vitro Fertilization (ivvitro Fertilization) refers to a technique In which sperm and eggs of a mammal complete the Fertilization process In an environment controlled manually In Vitro, abbreviated as IVF. The existing in vitro culture of human embryos is realized by placing a culture dish filled with embryos in a time difference incubator or a conventional carbon dioxide incubator for culture and simultaneously ensuring the temperature, humidity and cleanliness of the culture environment.
When a culture dish for culturing embryos is placed in an incubator, a culture solution for culturing embryos and a cover oil are added to the culture dish. In the prior art, usually, culture solution and cover oil are dropped into a culture dish in sequence by adopting a manual solution preparation mode, the liquid adding efficiency is low, and the requirement of batch embryo culture is difficult to meet.
SUMMERY OF THE UTILITY MODEL
Aiming at the technical problem that the automatic, intelligent and batch embryo culture requirements are difficult to meet by adopting manual liquid feeding during the existing human embryo culture; the utility model provides a liquid suction assembly for culturing biological tissues, which can be used for automatically sucking a biological tissue culture solution and meets the requirement of batch culture of the biological tissues.
The utility model is realized by the following technical scheme:
a wicking assembly for use in culturing biological tissue comprising: the lower end part of the insertion rod is used for inserting the liquid suction head, the insertion rod is provided with a suction channel used for connecting a negative pressure source, and the suction channel extends from the lower end surface of the insertion rod to the upper end of the insertion rod; the first linear driving mechanism is used for driving the insertion rod to move up and down; the pipe-releasing clamping jaw is in driving connection with the second linear driving mechanism and can move to the moving path of the insertion rod from the outer side of the insertion rod, so that the liquid suction head sleeved on the insertion rod is released relative to the pipe-releasing clamping jaw through the insertion rod.
When the device is used, the liquid suction assembly is carried on the manipulator, the suction channel in the insertion rod is connected with a negative pressure source (for example, connected with negative pressure generating equipment such as a plunger pump, a peristaltic pump and the like), the corresponding liquid suction cap is sleeved at the lower end part of the insertion rod, the manipulator moves the liquid suction assembly to the upper part of a culture solution storage container, then the insertion rod is driven to move downwards by the first linear driving mechanism, the lower end of the liquid suction head extends into the culture solution, and the negative pressure is pumped from the suction channel by the negative pressure source, so that the culture solution is sucked.
When the liquid suction head sleeved on the insertion rod needs to be taken down, the liquid suction assembly is moved to the upper position of a liquid suction head collection station by the manipulator, the insertion rod is driven to move downwards by the first linear driving mechanism, the upper end of the liquid suction head is located at the lower end of the pipe release clamping jaw, the pipe release clamping jaw is driven by the second linear driving mechanism to move outside the insertion rod to be clamped outside the insertion rod, the insertion rod is driven by the first linear driving mechanism to move up and down, the upper end of the liquid suction head is limited by the pipe release clamping jaw to enable the insertion rod to move relative to the liquid suction head, and the liquid suction head is removed from the insertion head.
In conclusion, the utility model can be used for automatically sucking the biological tissue culture solution and meets the requirement of batch culture of biological tissues.
In an alternative embodiment, the lower end of the insertion rod is in the shape of a stepped shaft with a large upper end and a small lower end, so that the liquid suction head can be sleeved at the lower end of the insertion rod.
In an alternative embodiment, the suction channel extends through the insertion rod to facilitate the arrangement of the negative pressure pipeline, and to avoid the pipeline interfering with the movement of the insertion rod.
In an optional embodiment, two insertion rods are arranged in parallel, and the two insertion rods are in driving connection with the corresponding first linear driving mechanism so as to meet the requirement of respectively sucking the culture solution and the cover oil.
In an optional embodiment, the first linear driving mechanism comprises a screw slider mechanism, so that the insertion rod is driven by the screw slider mechanism to move up and down, on one hand, the position precision of the movement of the insertion rod can be ensured, on the other hand, the insertion rod can be maintained at a set height, a brake assembly is not required, and the structure of the liquid suction assembly is simplified.
In an optional embodiment, the first linear driving mechanism further comprises a synchronous pulley mechanism, and the synchronous pulley mechanism is in transmission connection with the lead screw slider mechanism to ensure the response speed of the first linear driving mechanism.
In an optional embodiment, the pipe-removing claw is provided with a notch matched with the rod part of the insertion rod so as to ensure that the pipe-removing claw can limit the liquid suction head to move upwards along with the insertion rod.
In an alternative embodiment, the second linear drive mechanism is an electromagnet drive mechanism to simplify the construction and reduce the volume of the pipetting assembly.
In an optional embodiment, the liquid sucking device further comprises a liquid sucking head matched with the insertion rod.
The utility model has the following beneficial effects:
when the device is used, the insertion rod is driven to move downwards by the first linear driving mechanism, the lower end of the liquid suction head extends into the culture solution, negative pressure is pumped from the suction channel by the negative pressure source, so that the culture solution is sucked, the insertion rod moves relative to the pipe-removing clamping jaw to remove the liquid suction head sleeved on the insertion rod, and the automatic replacement of the liquid suction head can be realized.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present application and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained from the drawings without inventive effort.
FIG. 1 is a schematic perspective view of a wicking assembly for use in culturing biological tissue in accordance with an embodiment of the present invention;
FIG. 2 is a schematic side view of a wicking assembly for use in culturing biological tissue in accordance with an embodiment of the utility model;
fig. 3 is a schematic structural view of a pipe-removing jaw mechanism according to an embodiment of the utility model.
Reference numerals: 401-plug rod, 402-liquid suction head, 403-first linear driving mechanism, 404-pipe-removing claw and 405-second linear driving mechanism.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are some embodiments of the present application, but not all embodiments. The components of the embodiments of the present application, generally described and illustrated in the figures herein, can be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the present application, presented in the accompanying drawings, is not intended to limit the scope of the claimed application, but is merely representative of selected embodiments of the application. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.
In the description of the embodiments of the present application, the terms "central," "upper," "lower," "left," "right," "vertical," "longitudinal," "lateral," "horizontal," "inner," "outer," "front," "rear," "top," "bottom," and the like refer to orientations or positional relationships that are conventionally used in the manufacture of the present application, or that are routinely understood by those of ordinary skill in the art, but are merely used to facilitate the description and to simplify the description and do not indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be considered limiting of the present application.
In the description of the present invention, unless otherwise expressly specified or limited, the terms "disposed," "open," "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict.
Examples
With reference to fig. 1 and 2, the present embodiment provides a pipetting assembly for culturing biological tissue, comprising: the lower end of the insertion rod 401 is used for inserting a liquid suction head 402, the insertion rod 401 is provided with a suction channel used for connecting a negative pressure source, and the suction channel extends from the lower end of the insertion rod 401 to the upper end of the insertion rod 401; the first linear driving mechanism 403 is used for driving the plugging rod 401 to move up and down; the pipe stripping claw 404 is in driving connection with a second linear driving mechanism 405, the pipe stripping claw 404 can move from the outer side of the insertion rod 401 to the moving path of the insertion rod 401, and the liquid suction head 402 sleeved on the insertion rod 401 is removed from the pipe stripping claw 404 through the insertion rod 401.
With reference to fig. 1, the lower end of the insertion rod 401 is in a stepped shaft shape with a large top and a small bottom, so as to sleeve the liquid suction head 402 on the lower end of the insertion rod 401.
Preferably, the suction channel penetrates through the insertion rod 401, so that the arrangement of a negative pressure pipeline is facilitated, and the pipeline is prevented from interfering with the movement of the insertion rod 401.
Furthermore, two insertion rods 401 are arranged in parallel, and the two insertion rods 401 are in driving connection with the corresponding first linear driving mechanism 403 so as to meet the requirement of respectively sucking the culture solution and the cover oil. Correspondingly, the pipe-removing clamping jaw assemblies are also provided with two groups.
Referring to fig. 2, the first linear driving mechanism 403 includes a screw slider mechanism to drive the insertion rod 401 to move up and down through the screw slider mechanism, so that on one hand, the positional accuracy of the movement of the insertion rod 401 can be ensured, and on the other hand, the insertion rod 401 can be maintained at a set height without a brake assembly, thereby simplifying the structure of the liquid suction assembly.
Preferably, the first linear driving mechanism 403 further includes a synchronous pulley mechanism, and the synchronous pulley mechanism is in transmission connection with the lead screw slider mechanism to ensure the response speed of the first linear driving mechanism 403.
Referring to fig. 3, the tube removing claw 404 is provided with a recess matched with the rod part of the insertion rod 401 to ensure that the tube removing claw 404 can limit the liquid suction head 402 from moving upwards along with the insertion rod 401.
Preferably, the second linear drive mechanism 405 is an electromagnet drive mechanism to simplify the construction and reduce the volume of the pipetting assembly.
It will be appreciated that this embodiment also includes a fluid head 402 that is adapted to the insertion rod 401.
When the suction device is used, the liquid suction assembly is carried on the manipulator, the suction channel in the insertion rod 401 is connected with a negative pressure source (for example, connected with negative pressure generating equipment such as a plunger pump and a peristaltic pump), and the corresponding liquid suction head 402 is sleeved at the lower end part of the insertion rod 401 so that the inner cavity of the liquid suction head 402 is communicated with the suction channel. The manipulator moves the imbibing component 400 above the culture fluid storage and cover oil storage container, then drives a plug-in rod 401 to move downwards through a first linear driving mechanism 403, the lower end of the imbibing head 402 extends into the culture fluid, and then the negative pressure source pumps negative pressure to the imbibing channel, so that the culture fluid is imbibed; the other plug-in rod 401 is driven to move downwards by another first linear driving mechanism 403, the lower end of the liquid suction head 402 extends into the seal oil, and negative pressure is pumped out of the suction channel by a negative pressure source, so that the seal oil is sucked.
When the liquid suction head 402 sleeved on the insertion rod 401 needs to be taken down, the manipulator moves the liquid suction assembly to the position on the liquid suction head 402 collecting station, the insertion rod 401 is driven to move downwards through the first linear driving mechanism 403, the upper end of the liquid suction head 402 is located at the lower end of the tube disengaging jaw 404, the tube disengaging jaw 404 is driven by the second linear driving mechanism to be clamped outside the insertion rod 401 through the outer side movement of the insertion rod 401, then the insertion rod 401 is driven to move up and down through the first linear driving mechanism 403, the upper end of the liquid suction head 402 is limited through the tube disengaging jaw 404, so that the insertion rod 401 and the liquid suction head 402 move relatively, and the liquid suction head 402 is removed from the insertion head.
To sum up, this embodiment can be used for automatic absorption biological tissue culture solution, satisfies the demand that biological tissue batch culture was cultivateed.
The foregoing is only a preferred embodiment of the present invention, and the present invention is not limited thereto in any way, and any simple modification, equivalent replacement and improvement made to the above embodiment within the spirit and principle of the present invention still fall within the protection scope of the present invention.

Claims (9)

1. A wicking assembly for use in culturing biological tissue, comprising:
the lower end of the insertion rod (401) is used for inserting a liquid suction head (402), the insertion rod (401) is provided with a suction channel used for connecting a negative pressure source, and the suction channel extends from the lower end of the insertion rod (401) to the upper end of the insertion rod (401);
the first linear driving mechanism (403) is used for driving the plugging rod (401) to move up and down;
the pipe-removing clamping jaw (404) is in driving connection with a second linear driving mechanism (405), and the pipe-removing clamping jaw (404) can move to the moving path of the insertion rod (401) from the outer side of the insertion rod (401) so as to remove the liquid suction head (402) sleeved on the insertion rod (401) relative to the pipe-removing clamping jaw (404) through the insertion rod (401).
2. Pipetting assembly for cultivating biological tissue as in claim 1, characterized in that the lower end of the rod (401) is in the form of a stepped shaft with a large top and a small bottom.
3. Pipetting assembly for cultivating biological tissue according to claim 1, characterized in that the aspiration channel extends through the insertion rod (401).
4. Pipetting assembly according to any one of claims 1-3, characterized in that two of the insertion rods (401) are arranged side by side and that both insertion rods (401) are drivingly connected to the corresponding first linear drive (403).
5. Pipetting assembly for cultivating biological tissue according to claim 4, characterized in that the first linear drive mechanism (403) comprises a screw-and-slider mechanism.
6. Pipetting assembly for cultivating biological tissue according to claim 5, characterized in that the first linear drive mechanism (403) further comprises a synchronous pulley mechanism in driving connection with a lead screw-slider mechanism.
7. Pipetting assembly for cultivating biological tissue according to claim 1, characterized in that the tube-removing jaws are provided with recesses adapted to the stem of the insertion rod (401).
8. Pipetting assembly for cultivating biological tissue according to claim 1, characterized in that the second linear drive (405) is an electromagnet drive.
9. Pipetting assembly for cultivating biological tissue according to claim 1, further comprising a pipetting head (402) adapted to the insertion rod (401).
CN202122133011.5U 2021-09-03 2021-09-03 Liquid suction assembly for culturing biological tissue Active CN215560387U (en)

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Application Number Priority Date Filing Date Title
CN202122133011.5U CN215560387U (en) 2021-09-03 2021-09-03 Liquid suction assembly for culturing biological tissue

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Application Number Priority Date Filing Date Title
CN202122133011.5U CN215560387U (en) 2021-09-03 2021-09-03 Liquid suction assembly for culturing biological tissue

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CN215560387U true CN215560387U (en) 2022-01-18

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113717852A (en) * 2021-09-03 2021-11-30 重庆市盛佰昱科技有限公司 Liquid suction method and liquid feeding method for biological tissue culture solution

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113717852A (en) * 2021-09-03 2021-11-30 重庆市盛佰昱科技有限公司 Liquid suction method and liquid feeding method for biological tissue culture solution

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