CN105230468A - System and method for producing detoxified lily bulb seed balls in large scale - Google Patents
System and method for producing detoxified lily bulb seed balls in large scale Download PDFInfo
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- CN105230468A CN105230468A CN201510736398.XA CN201510736398A CN105230468A CN 105230468 A CN105230468 A CN 105230468A CN 201510736398 A CN201510736398 A CN 201510736398A CN 105230468 A CN105230468 A CN 105230468A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
- A01G31/06—Hydroponic culture on racks or in stacked containers
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G2031/006—Soilless cultivation, e.g. hydroponics with means for recycling the nutritive solution
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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- Botany (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a system for producing detoxified lily bulb seed balls in large scale. The system comprises a culture solution reflux tank (1), a culture solution storage tank (2) and an atomizing culture box (3) which are sequentially connected, wherein the atomizing culture box (3) is connected with an inlet of the culture solution reflux tank (1) through a first pipeline (10), and the culture solution reflux tank (1) is connected with a high pressure air supply device. The invention further discloses a method for producing the detoxified lily bulb seed balls through the system for producing the detoxified lily bulb seed balls in the large scale. The system and the method have the advantages of being short in transformation period, low in pollution rate, low in cost and simple and convenient to operate.
Description
Technical field
The present invention relates to biological technical field, particularly relate to a kind of system and method for large-scale production lily detoxification seed ball.
Background technology
Lily is the perennial herb bulbous plant of Liliaceae lilium, originates in China, and be mainly distributed in the Temperate Region in Chinas, the Northern Hemisphere such as east Asia, Europe, North America, the whole world has found that there is at least 120 kinds, and wherein 55 kinds originate in China.In recent years the new varieties much produced through artificial hybridization are more had, as Lilium Pollyanna, Fragrant Lily, fiery lily etc.Bulb is containing enriching starch, edible, also does medicinal.Lily eats dry using raw.Lily is Chinese tradition outlet special product.
Lily is all the world's famous flower liked by people from ancient times to the present, and lily appearance is graceful, and blade is verdant graceful, and stem is slim and graceful, is famous and precious cut-flower rising star, has the connotation of the fine family of life-long happiness and perfect harmony, greatest love of all Chinese lily, has the meaning of deep blessing.
The existing propagation method of lily mainly contains scale lining, the breeding of seed ball and bulbil and breeds 3 kinds in production, there is reproductive efficiency slow, lower or the clove of balling ratio takes root negligible amounts, reproduction coefficient is low, and be subject to the shortcoming of natural conditions restriction, along with the development of biotechnology, tissue culture technique improves reproductive efficiency to a certain extent, but still there is complicated operation, the shortcoming such as cost has high input, pollution rate.The demand in enormous quantities in production process can not be met.
Summary of the invention
The technical problem to be solved in the present invention is to provide the system of the large-scale production lily detoxification seed ball that a kind of transformation period is short, pollution rate is low, cost is low, easy and simple to handle and adopts this system to produce the method for lily detoxification seed ball.
A system for large-scale production lily detoxification seed ball, comprises the culture fluid return tank, culture fluid holding vessel and the atomization incubator that are connected successively; Described atomization incubator is connected with the entrance of described culture fluid return tank by the first pipeline, and described culture fluid return tank is connected with pressure-air generator.
The system of large-scale production lily detoxification seed ball of the present invention; wherein; described culture fluid return tank is provided with float-controlled valve; the first valve is provided with between described culture fluid return tank and described pressure-air generator; pipeline between described culture fluid return tank and described culture fluid holding vessel is disposed with culture fluid filter and the second valve; be connected by the second pipeline between described culture fluid holding vessel and described atomization incubator; described second pipeline is provided with the 3rd valve, described first pipeline is provided with the 4th valve.
The system of large-scale production lily detoxification seed ball of the present invention, wherein, described first valve is air-operated solenoid valve, and described second valve and described 4th valve are check valve, and described 3rd valve is aqueous phase magnetic valve.
The system of large-scale production lily detoxification seed ball of the present invention, wherein, described atomization incubator comprises support body and top board fixed thereon, base plate, front side wall, rear wall, left side wall and right side wall, culturing rack is comprised in described atomization incubator, described culturing rack is made up of multiple object placement nets parallel up and down, for removably to connect between multiple described object placement net, the bottom of described top board is provided with at least one water inlet pipe, multiple atomizer is fixedly connected with described water inlet pipe, described multiple atomizer is evenly distributed on the bottom of described top board, described water inlet pipe is connected with described second pipeline,
In the bottom of described culturing rack, the top of described base plate is provided with water collection sheet, described water collection sheet is rectangle, and be that axis upwards turns down that to form cross section be the cell body of V-shaped along the center line of described rectangular length direction, described water collection sheet tilts to be fixed on the bottom of described culturing rack, bottom the cell body of described lower side, be provided with semicircular water collector, the bottom of described water collector is connected with outlet pipe, and described outlet pipe is connected with described first pipeline.
The system of large-scale production lily detoxification seed ball of the present invention, wherein, the angle between the center line of described water collection sheet and horizontal plane is 10 ~ 15 °; The cross section of described base plate is right angled triangle, is arranged on the bottom of described water collection sheet, and the bottom surface of described base plate is horizontal plane, and upper surface tilts, and incline direction is consistent with the incline direction of described water collection sheet; Described object placement net is provided with cultivation matrix, and thickness is 2 ~ 3cm, and described cultivation matrix is haydite;
Described top board, described base plate, described front side wall, described rear wall, described left side wall and described right side wall are made by transparent material, for being flexibly connected between described front side wall with described support body; Have blue-violet light source in the external stability of described atomization incubator, described blue-violet light source is the LED sending blue light, in netted layout;
Described second pipeline is connected with described water inlet pipe with described water collection sheet through described base plate, and described second pipeline is welded and fixed with described base plate and described water collection sheet respectively.
Adopt the system of large-scale production lily detoxification seed ball of the present invention to produce the method for lily detoxification seed ball, comprise the steps:
The cultivation matrix putting into the atomization incubator of the system of large-scale production lily detoxification seed ball after being cleaned up by lily bud scale is cultivated, 13-15 days, lily bud scale base portion can sprout indefinite bud, within 45 days, can obtain lily detoxification seed ball;
Described incubation adopts spray process, carries out described spray processing procedure at every turn and comprises the steps:
Step one, culture fluid filter and storage of higher pressures: the first valve open, now float-controlled valve and the 4th valve are closed because of gases at high pressure effect, culture fluid in culture fluid return tank is released by the 4MPa gases at high pressure provided by gases at high pressure generator, through culture fluid filter and the second valve, enter culture fluid holding vessel and store; In this process, 3rd valve is in closed condition, this process duration is 5 minutes, i.e. described first valve opening 5 minutes, after 5 minutes, first valve closing, cutoff high gas, meanwhile, described culture fluid return tank is reduced to normal atmosphere (An) rapidly, described float-controlled valve declines, and described culture fluid return tank communicates with ambient atmosphere;
Step 2, the spraying of atomization incubator: according to frequency and the time of setting, described 3rd valve opening, the culture fluid that described culture fluid holding vessel mesohigh stores enters the atomizer of atomization incubator through described 3rd valve, culture fluid is atomized, this process duration 5 minutes, after 5 minutes, described 3rd valve closing;
Culture fluid backflow in step 3, atomization incubator: after atomization incubator has been sprayed, the V-shaped tank of culture fluid through bottom flows in water collector, and the 4th valve opening, culture fluid enters described culture fluid return tank, this process duration 15 minutes.
The method of production lily detoxification seed ball of the present invention, described incubation comprises 4 stages:
First stage: the time is the 1st day ~ the 6th day, 0 point of every day, 4 points, 7 points, 11 points, 15 and 20 totally 6 time points, once spray process respectively at each time point, the culture fluid of employing is MS+0.2mg/LGA3+2mg/L6-BA+1000mg/L carbendazim;
Second stage: the time is the 7th day ~ the 20th day, 0 point of every day, 5 points, 9 points, 13 and 18 totally 5 time points, carry out twice spray process respectively at each time point, the culture fluid of employing is MS+0.05mg/LTDZ+1.8mg/L6-BA+200mg/L paclobutrazol;
Phase III: the time is the 21st day ~ the 35th day, 3 points of every day, 7 points, 13 and 20 totally 4 time points, carry out twice spray process respectively at each time point, the culture fluid of employing is MS+0.15mg/LNAA+0.8mg/L6-BA+300mg/L carbendazim;
Fourth stage: the time is the 36th day ~ the 45th day, 0 point of every day, 4 points, 7 points, 10 points, 15 and 19 totally 6 time points, once spray process respectively at each time point, the culture fluid of employing is MS+0.4mg/LTDZ+1.2mg/L6-BA+50mg/L paclobutrazol.
The system difference from prior art of large-scale production lily detoxification seed ball of the present invention is:
The system tool of large-scale production lily detoxification seed ball of the present invention has the following advantages:
1) incubation of the present invention is all operate under open environment, does not limit by environmental condition, easy and simple to handle;
2) traditional incubation workload is large, and reproduction rate is low, and tissue culture procedures workload is large, pollution rate is high, be unfavorable for large-scale production, and workload of the present invention little, workable and can realize produce in enormous quantities, there is not again pollution problem in open cultivation;
3) the present invention adopts spray processing method to make the nutrient of nutrient solution obtain efficiency utilization, open cultivation, and better promote the growth of seedling, do not need a lot of matrix in incubation, floor space is little;
The method of the invention is with short production cycle, substantially increases efficiency and productivity ratio.
The present invention can also adopt royal purple light to carry out light filling, mainly promotes the differentiation of plant cell, and promotes that plant elongation is formed with young shoot; The structure of atomization incubator of the present invention is unique design; pressure-air generator, culture fluid return tank, culture fluid filter, culture fluid holding vessel and each valve are all prior aries; it is connected with the atomization incubator of unique design with special connected mode by of the present invention focusing on; form the system of large-scale production lily detoxification seed ball of the present invention; the availability of nutrient solution can be improved, utilize illumination condition and greenhouse space to greatest extent.And be beneficial to operation, convenient management.
System of the present invention is adopted to produce lily detoxification seed ball, 4 stages are divided to carry out, different medium, different spray times and spray mode is adopted to cultivate at the operational phase of different time points residing for lily bud scale explant, reproductive efficiency is high, do not pollute, take up an area little, significantly reduce working strength.
Be described further below in conjunction with the system of accompanying drawing to large-scale production lily detoxification seed ball of the present invention.
Accompanying drawing explanation
Fig. 1 is the structural representation of the system of large-scale production lily detoxification seed ball of the present invention;
Fig. 2 is the structural representation being atomized incubator in the system of large-scale production lily detoxification seed ball of the present invention;
Fig. 3 is the structural representation that the present invention is atomized water collection sheet in incubator.
Embodiment
Embodiment 1
As shown in FIG. 1 to 3, the system of large-scale production lily detoxification seed ball of the present invention comprises the culture fluid return tank 1, culture fluid holding vessel 2 and the atomization incubator 3 that are connected successively; Atomization incubator 3 is connected with the entrance of culture fluid return tank 1 by the first pipeline 10, and culture fluid return tank 1 is connected with pressure-air generator.
Culture fluid return tank 1 is provided with float-controlled valve 4, the first valve 5 is provided with between culture fluid return tank 1 and pressure-air generator, pipeline between culture fluid return tank 1 and culture fluid holding vessel 2 is disposed with culture fluid filter 6 and the second valve 7, be connected by the second pipeline 11 between culture fluid holding vessel 2 and atomization incubator 3, second pipeline 11 is provided with the 3rd valve 8, the first pipeline 10 is provided with the 4th valve 9.First valve 5 is air-operated solenoid valve, and the second valve 7 and the 4th valve 9 are check valve, and the 3rd valve 8 is aqueous phase magnetic valve.
Atomization incubator 3 comprises support body and top board fixed thereon, base plate, front side wall, rear wall, left side wall and right side wall, culturing rack 301 is comprised in atomization incubator 3, culturing rack 301 is made up of multiple object placement nets 302 parallel up and down, for removably to connect between multiple object placement net 302, at least one water inlet pipe 303 is provided with in the bottom of top board, multiple atomizer 304 is fixedly connected with water inlet pipe 303, multiple atomizers 304 are evenly distributed on the bottom of top board, and water inlet pipe 303 is connected with the second pipeline 11; The quantity of water inlet pipe 303 and atomizer 304 with can equably irrigating plant for standard, arbitrary quantity can be set, such as 8,10,20 etc., when plant is many, also can all arrange water inlet pipe 303 and atomizer 304 on the top of each layer commodity net 302, all water inlet pipes 303 are all connected with the second pipeline 11 respectively by pipeline.
In the bottom of culturing rack 301, the top of base plate is provided with water collection sheet 305, water collection sheet 305 is rectangle, and be that upwards to turn down that to form cross section be the cell body of V-shaped (be alternatively the square plate body of two block lengths to axis along the center line of rectangular length direction, a limit of the length direction of the two is connected, acutangulate between the plate face of the square plate body of two block lengths), water collection sheet 305 tilts to be fixed on the bottom of culturing rack 301, semicircular water collector 306 is provided with bottom the cell body of lower side, the bottom of water collector 306 is connected with outlet pipe 307, outlet pipe 307 is connected with the first pipeline 10.
Angle between the center line of water collection sheet 305 and horizontal plane is 10 ~ 15 °, is gathered in water collector 306 more easily more smoothly to make the culture fluid stayed; The cross section of base plate is right angled triangle, is arranged on the bottom of water collection sheet 305, and the bottom surface of base plate is horizontal plane, and upper surface tilts, and incline direction is consistent with the incline direction of water collection sheet 305; Object placement net 302 is provided with cultivation matrix, and thickness is 2 ~ 3cm, and cultivation matrix is haydite;
Top board, base plate, front side wall, rear wall, left side wall and right side wall are made by transparent material, for being flexibly connected between front side wall with support body; Have blue-violet light source in the external stability of atomization incubator 3, blue-violet light source is the LED sending blue light, in netted layout;
Second pipeline 11 is connected with water inlet pipe 303 with water collection sheet 305 through base plate, and the second pipeline 11 is welded and fixed with base plate and water collection sheet 305 respectively, between for being tightly connected, culture fluid can not be revealed, and facilitates the hiding, more attractive in appearance of pipeline.
Adopt the system of large-scale production lily detoxification seed ball of the present invention to produce the method for lily detoxification seed ball, comprise the steps:
The cultivation matrix putting into the atomization incubator 3 of the system of large-scale production lily detoxification seed ball after being cleaned up by lily bud scale is cultivated, 13-15 days, lily bud scale base portion can sprout indefinite bud, within 45 days, can obtain lily detoxification seed ball;
Incubation adopts spray process, carries out spray processing procedure at every turn and comprises the steps:
Step one, culture fluid filter and storage of higher pressures: the first valve 5 is opened, now float-controlled valve 4 and the 4th valve 9 are closed because of gases at high pressure effect, culture fluid in culture fluid return tank 1 is released by the 4MPa gases at high pressure provided by gases at high pressure generator, through culture fluid filter 6 and the second valve 7, enter culture fluid holding vessel 2 and store; In this process, 3rd valve 8 is in closed condition, this process duration is 5 minutes, and namely the first valve 5 opens 5 minutes, after 5 minutes, first valve 5 is closed, cutoff high gas, meanwhile, culture fluid return tank 1 is reduced to normal atmosphere (An) rapidly, float-controlled valve 4 declines, and culture fluid return tank 1 communicates with ambient atmosphere;
Step 2, atomization incubator 3 are sprayed: according to frequency and the time of setting, 3rd valve 8 is opened, the culture fluid that culture fluid holding vessel 2 mesohigh stores enters the atomizer 304 of atomization incubator 3 through the 3rd valve 8, culture fluid is atomized, this process duration 5 minutes, after 5 minutes, the 3rd valve 8 is closed;
Culture fluid backflow in step 3, atomization incubator 3: after atomization incubator 3 has been sprayed, the V-shaped tank of culture fluid through bottom flows in water collector 306, and the 4th valve 9 is opened, and culture fluid enters culture fluid return tank 1, this process duration 15 minutes.
Wherein, incubation comprises 4 stages:
First stage: the time is the 1st day ~ the 6th day, 0 point of every day, 4 points, 7 points, 11 points, 15 and 20 totally 6 time points, once spray process respectively at each time point, the culture fluid of employing is MS+0.2mg/LGA3+2mg/L6-BA+1000mg/L carbendazim;
Second stage: the time is the 7th day ~ the 20th day, 0 point of every day, 5 points, 9 points, 13 and 18 totally 5 time points, carry out twice spray process respectively at each time point, the culture fluid of employing is MS+0.05mg/LTDZ+1.8mg/L6-BA+200mg/L paclobutrazol;
Phase III: the time is the 21st day ~ the 35th day, 3 points of every day, 7 points, 13 and 20 totally 4 time points, carry out twice spray process respectively at each time point, the culture fluid of employing is MS+0.15mg/LNAA+0.8mg/L6-BA+300mg/L carbendazim;
Fourth stage: the time is the 36th day ~ the 45th day, 0 point of every day, 4 points, 7 points, 10 points, 15 and 19 totally 6 time points, once spray process respectively at each time point, the culture fluid of employing is MS+0.4mg/LTDZ+1.2mg/L6-BA+50mg/L paclobutrazol.
Above embodiment is only be described the preferred embodiment of the present invention; not scope of the present invention is limited; under not departing from the present invention and designing the prerequisite of spirit; the various distortion that those of ordinary skill in the art make technical scheme of the present invention and improvement, all should fall in protection domain that claims of the present invention determines.
Claims (7)
1. a system for large-scale production lily detoxification seed ball, is characterized in that: comprise culture fluid return tank (1) connected successively, culture fluid holding vessel (2) and atomization incubator (3); Described atomization incubator (3) is connected by the entrance of the first pipeline (10) with described culture fluid return tank (1), and described culture fluid return tank (1) is connected with pressure-air generator.
2. the system of large-scale production lily detoxification seed ball according to claim 1, it is characterized in that: described culture fluid return tank (1) is provided with float-controlled valve (4), the first valve (5) is provided with between described culture fluid return tank (1) and described pressure-air generator, pipeline between described culture fluid return tank (1) and described culture fluid holding vessel (2) is disposed with culture fluid filter (6) and the second valve (7), be connected by the second pipeline (11) between described culture fluid holding vessel (2) and described atomization incubator (3), described second pipeline (11) is provided with the 3rd valve (8), described first pipeline (10) is provided with the 4th valve (9).
3. the system of large-scale production lily detoxification seed ball according to claim 2; it is characterized in that: described first valve (5) is air-operated solenoid valve; described second valve (7) and described 4th valve (9) are check valve, and described 3rd valve (8) is aqueous phase magnetic valve.
4. the system of the large-scale production lily detoxification seed ball according to claim 1 or 2 or 3, it is characterized in that: described atomization incubator (3) comprises support body and top board fixed thereon, base plate, front side wall, rear wall, left side wall and right side wall, culturing rack (301) is comprised in described atomization incubator (3), described culturing rack (301) is made up of multiple object placement nets (302) parallel up and down, for removably to connect between multiple described object placement net (302), at least one water inlet pipe (303) is provided with in the bottom of described top board, multiple atomizer (304) is fixedly connected with described water inlet pipe (303), multiple described atomizers (304) are evenly distributed on the bottom of described top board, described water inlet pipe (303) is connected with described second pipeline (11),
In the bottom of described culturing rack (301), the top of described base plate is provided with water collection sheet (305), described water collection sheet (305) is rectangle, and be that axis upwards turns down that to form cross section be the cell body of V-shaped along the center line of described rectangular length direction, described water collection sheet (305) tilts to be fixed on the bottom of described culturing rack (301), semicircular water collector (306) is provided with bottom the cell body of described lower side, the bottom of described water collector (306) is connected with outlet pipe (307), described outlet pipe (307) is connected with described first pipeline (10).
5. the system of large-scale production lily detoxification seed ball according to claim 4, is characterized in that: the angle between the center line of described water collection sheet (305) and horizontal plane is 10 ~ 15 °; The cross section of described base plate is right angled triangle, is arranged on the bottom of described water collection sheet (305), and the bottom surface of described base plate is horizontal plane, and upper surface tilts, and incline direction is consistent with the incline direction of described water collection sheet (305); Described object placement net (302) is provided with cultivation matrix, and thickness is 2 ~ 3cm, and described cultivation matrix is haydite;
Described top board, described base plate, described front side wall, described rear wall, described left side wall and described right side wall are made by transparent material, for being flexibly connected between described front side wall with described support body; Have blue-violet light source in the external stability of described atomization incubator (3), described blue-violet light source is the LED sending blue light, in netted layout;
Described second pipeline (11) is connected with described water inlet pipe (303) with described water collection sheet (305) through described base plate, and described second pipeline (11) is welded and fixed with described base plate and described water collection sheet (305) respectively.
6. adopt the system of large-scale production lily detoxification seed ball according to claim 4 to produce the method for lily detoxification seed ball, it is characterized in that: comprise the steps:
The cultivation matrix putting into the atomization incubator (3) of the system of large-scale production lily detoxification seed ball after being cleaned up by lily bud scale is cultivated, 13-15 days, lily bud scale base portion can sprout indefinite bud, within 45 days, can obtain lily detoxification seed ball;
Described incubation adopts spray process, carries out described spray processing procedure at every turn and comprises the steps:
Step one, culture fluid filter and storage of higher pressures: the first valve (5) is opened, now float-controlled valve (4) and the 4th valve (9) are closed because of gases at high pressure effect, culture fluid in culture fluid return tank (1) is released by the 4MPa gases at high pressure provided by gases at high pressure generator, through culture fluid filter (6) and the second valve (7), enter culture fluid holding vessel (2) and store; In this process, 3rd valve (8) is in closed condition, this process duration is 5 minutes, and namely described first valve (5) is opened 5 minutes, after 5 minutes, first valve (5) is closed, cutoff high gas, meanwhile, described culture fluid return tank (1) is reduced to normal atmosphere (An) rapidly, described float-controlled valve (4) declines, and described culture fluid return tank (1) communicates with ambient atmosphere;
Step 2, atomization incubator (3) spraying: according to frequency and the time of setting, described 3rd valve (8) is opened, the culture fluid that described culture fluid holding vessel (2) mesohigh stores enters the atomizer (304) of atomization incubator (3) through described 3rd valve (8), culture fluid is atomized, this process duration 5 minutes, after 5 minutes, described 3rd valve (8) is closed;
Culture fluid backflow in step 3, atomization incubator (3): after atomization incubator (3) has been sprayed, the V-shaped tank of culture fluid through bottom flows in water collector (306), 4th valve (9) is opened, culture fluid enters described culture fluid return tank (1), this process duration 15 minutes.
7. adopt the method for production lily detoxification seed ball according to claim 6, it is characterized in that: described incubation comprises 4 stages:
First stage: the time is the 1st day ~ the 6th day, 0 point of every day, 4 points, 7 points, 11 points, 15 and 20 totally 6 time points, once spray process respectively at each time point, the culture fluid of employing is MS+0.2mg/LGA3+2mg/L6-BA+1000mg/L carbendazim;
Second stage: the time is the 7th day ~ the 20th day, 0 point of every day, 5 points, 9 points, 13 and 18 totally 5 time points, carry out twice spray process respectively at each time point, the culture fluid of employing is MS+0.05mg/LTDZ+1.8mg/L6-BA+200mg/L paclobutrazol;
Phase III: the time is the 21st day ~ the 35th day, 3 points of every day, 7 points, 13 and 20 totally 4 time points, carry out twice spray process respectively at each time point, the culture fluid of employing is MS+0.15mg/LNAA+0.8mg/L6-BA+300mg/L carbendazim;
Fourth stage: the time is the 36th day ~ the 45th day, 0 point of every day, 4 points, 7 points, 10 points, 15 and 19 totally 6 time points, once spray process respectively at each time point, the culture fluid of employing is MS+0.4mg/LTDZ+1.2mg/L6-BA+50mg/L paclobutrazol.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106613811A (en) * | 2016-09-21 | 2017-05-10 | 新疆生产建设兵团第六师农业科学研究所 | Improved tulip atomization promoting cultivation method |
| CN107890705A (en) * | 2017-12-22 | 2018-04-10 | 郑州玖意优创商贸有限公司 | Collapsible fresh water collecting car |
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| CN107890705A (en) * | 2017-12-22 | 2018-04-10 | 郑州玖意优创商贸有限公司 | Collapsible fresh water collecting car |
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