CN1142534A - Reactor for nutrient liquid atomizing culture of plant - Google Patents
Reactor for nutrient liquid atomizing culture of plant Download PDFInfo
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- CN1142534A CN1142534A CN 96105058 CN96105058A CN1142534A CN 1142534 A CN1142534 A CN 1142534A CN 96105058 CN96105058 CN 96105058 CN 96105058 A CN96105058 A CN 96105058A CN 1142534 A CN1142534 A CN 1142534A
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Abstract
A plant cultivating reactor atomizing nutritive liquid features that the nutritive liquid is atomized by ultrasonic wave or pressure nozzle and then diffused in the whole reactor. The system uses an integral structure including cultivation case, atomizer and storage tank. The atomized nutritive liquid is diffused under the action of rotating propeller and tissue blocks are put on multilayer stainless steel net in the reactor. Atomizing and introducing air in are alternative.
Description
The present invention relates to plant tissue cell and complete plant culturing, relate in particular to the reactor for nutrient liquid atomizing culture of plant.
In this field of plant culturing, especially aspect tissue culture, very suitable equipment and device (book of originally telling somebody what one's real intentions are is referred to as bio-reactor) are not arranged so far.And culture medium also just adopts liquid or be added with two kinds of forms of solid medium of agar.
The current cultural method that generally adopts is: in little culturing bottle, with the liquid nutrient medium submergence tissue block of shallow-layer or with tissue block fixing cultivation on solid medium.Place constant temperature culture indoor on little culturing bottle, suitably control illumination condition well.
Design is suitable for the new-type bioreactor of plant tissue culture, is the industrialized key link of this technological direction, and many researchists have done a large amount of work in this respect.Report was once arranged, stirring-type or airlift reactor were used for the cultivation of plant tissue such as embryoid.In addition, some investigators have designed novel reactor miscellaneous according to the characteristics of plant tissue, cell cultures, rotating drum type reactor for example, hanging stick stirring reactor.
Late nineteen eighties, Weathers and Giles have been reported with the experimental result of nutrient liquid atomizing culturing plants tissue, have been pointed out that this is a kind of promising novel method.What they used at that time is mechanical spraying, and device the results are shown in Figure 1.Subsequently, Weathers and co-worker thereof use supersonic atomizer to cultivate the various plants tissue again, have obtained good result, and system constitutes sees Fig. 2.Enter the nineties, the researchist of Japan and Korea S has also done the work of this respect, and people's such as the formation of their thinking and system and Weathero is basic identical, sees Fig. 3, and Fig. 4.
The many problems that exist in the prior art have limited the development of plant tissue culture to industrialization, mass-producing direction, and most of work rests on the level of simple and loaded down with trivial details work of bottle Bottle ﹠ Can jar.
Traditional little culturing bottle training method, the manpower consumption is big, complex operation.In culturing process, condition is not easy unified control between bottle and the bottle, thereby the synchronism of material is relatively poor.If use solid medium, the interpolation of agar have increased the cost of cultivating, and on solid medium, the growth of plant tissue, reproduction speed is slower; Utilization of nutrients is low; And the use liquid nutrient medium, owing to can only cultivate by shallow-layer, intermediate demand repeatedly changes liquid or additional fresh nutrient solution, has increased the fussy degree of work and the repeatedly unlatching of bottle cap, has enlarged the chance of microbiological contamination.
In the development and the application facet of bio-reactor, many investigators are according to plant tissue, the characteristics of cell cultures, or directly use traditional air lift type or stirred-tank fermenter; Or legacy equipment improved; Or designing novel bio-reactor, their common ground is: liquid nutrient medium submergence tissue block then makes system even by stirring or ventilating, and makes air feed abundant.This submergence mode is brought following several problem:
1, liquid-immersed is not the normal habitat of most of plants, and long-term submergence meeting produces vitrifaction, and this is a very serious problem that influences the liquid culture effect.
2, in the liquid culture process, the air feed weak effect, dissolved oxygen is low.And the plant tissue block is long-pending big, and specific surface area is much smaller with respect to unicellular, so internal diffusion transmission resistance is big, and this has just given prominence to the relatively poor problem of liquid culture air feed effect more.In addition, the existence of tissue block makes the flow effect of system relatively poor, has strengthened oxygen in the alternate transmission difficulty of gas, liquid, solid three, has reduced the efficient of air feed.
3, owing to need ceaselessly to stir or ventilation.On the one hand, bigger shearing force is arranged, tissue, cell are had destruction; On the other hand, tissue block is in not stationary state all the time, the directivity of eliminated its growth, growing, and experiment shows: plant-growth, growth have certain polar effect, and stationary state is more favourable.
The atomizing cultural method that Weathers etc. propose for plant tissue culture provides a new approach, and demonstrates bright development prospect.But Weathers and other investigators' the starting point is still swung at plant tissue in the physiology word research under the atomizing culture condition, and their culture systems is tentative, also has many problems aspect practical application and the amplification:
1, atomisation unit, incubator and nutritive medium basin separate, between link to each other with pipeline.This multitube road, multi-joint connecing tend to increase the chance of microbiological contamination, and are easy to generate the dead angle in sterilization process.
2, nutrient mist is brought incubator into sterile air, along with the discharge of tail gas, has the nutritive medium that is atomized in a large number and is pulled away, and causes waste, and deals with improperly and can cause environmental pollution.
3, for handling the nutrient mist in the tail gas, need suitable separating device, device are installed, increased the complexity of system.
More than these problems, along with the amplification of reactor will be more outstanding.
The present invention is directed to these problems that exist in the prior art, proposed a new thinking and the brand-new design of a cover.Its objective is: tissue culture is carried out with new-type bioreactor, realize reducing labour intensity on a large scale, simplify control condition.2. realize the immobilization cultivation, keep the polar effect of plant-growth, growth.3. solve vitrifying problem and air feed problem in the culturing process, eliminate the physical abuse effect.4. simplify the structure of culture systems, reduce the microbiological contamination chance, the difficulty that reduction equipment amplifies makes it practical more.5. make full use of plant tissue, the characteristics of cell growth and the characteristics on the reactor self structure simplify the operation, and save the energy, reduce the waste of nutritive substance and improve the effect of cultivating.
The concrete solution of the present invention is:
1, the reactor for nutrient liquid atomizing culture of a kind of plant.It is characterized in that: this reactor comprises: the stainless steel kerve 8 of glass tank body 1, Stainless Steel Cloth 2, tissue block 3, inner core 4, water screw 5, electromagnetic driver 6, top cover 7, strap clamp cover, atomizing transducing sheet 9, high-frequency oscillating circuits 10, time controller 11, strainer 12, fluorescent lamp 13, wherein, and electromagnetic driver 6 is arranged on the central authorities of top cover 7; The driving part of electromagnetic driver 6 places outside the jar, and follower is sealed in the jar, is added with water screw on the driven shaft; Top cover 7 is arranged on the glass tank body 1; Placement of multiple layers stainless (steel) wire 2 in the glass tank body 1, plant tissue piece 3 places on the net, glass tank body 1 is arranged on the stainless steel kerve 8, and with flange connection, central inner core 4 is arranged in the glass tank body 1 between stainless steel kerve 8 and the glass tank body 1, stainless steel kerve 8 bottoms, its with the transducing sheet of propeller and bottom point-blank, there is a circular hole in the central authorities of stainless steel kerve 8, atomizing transducing sheet 9 is fixed on outside jar on this hole, and strict seal; Stainless steel kerve 8 has chuck; High-frequency oscillating circuits 10 is connected with atomizing transducing sheet 9, and time controller 11 is connected with high-frequency oscillating circuits, and strainer 12 is connected with the inlet mouth of kerve 8, and fluorescent lamp 13 is arranged on outside the glass tank body 1.
Basic ideas of the present invention and method are: (with nutritive medium 1 or title liquid nutrient medium) atomizing, the means of atomizing are to use ultrasonic atomizer or the spraying of mechanical pressure shower nozzle, by diffusion, the sedimentation of mist, nutritive substance are filled the air to whole culture apparatus.This invention has the following advantages:
1. because atomizing can be propagated nutritive substance in gas phase, its flow effect is better than liquid, thereby reach even easilier.
2. tissue block is immersed in the aerosol, and nutrition supply and ventilation are all very abundant, has solved the difficult problem of liquid culture air feed, and has eliminated vitrification phenomenon.
3. temperature can be regulated by the temperature of control inlet air temperature and nutrient mist, and the control of the temperature of mist can realize by the temperature of regulating nutritive medium.
4. the higher humidity in the reactor that atomizes has strong bacterium to act on to the cultivations of some detoxification bacterium.
2, culture systems adopts spraying gun, incubator and the incorporate structure of basin.Stainless steel or vitreous inner core in one thin footpath are arranged in the tank body, and it is gone to the bottom and is higher than liquid level, and the transducing sheet at the bottom of the jar suitable for readingly is lower than tank deck, over against water screw.Nutrient mist produces in tube and by propeller-driven, forms circulation in jar, is full of whole device.Water screw is driven by the electromagnetic driver of tank deck, and rotating speed is adjustable, and we are called inner core circulation atomizing reactor with whole device, and detailed structure is seen Fig. 5, and its advantage is:
1. integral structure is simple, connects to lack, and has reduced the possibility of microbiological contamination, and amplification is realized easily, and the system after the amplification is easier to be reached evenly, is far superior to fixed bed and liquid culture reactor in this respect.
2. because the suspension effect of mist is good, easily diffusion, propagation so available low power electromagnetic driver drives water screw, drive the mist circulation; And ventilation also only needs low discharge, so energy consumption is low.
3. water screw drives with the outer electromagnetic driver of tank body, and seamless, good sealing effect is difficult for microbiological contamination.
4. reactor is equipped with inner core, and on the one hand, because its diameter is very little, it is little to take volume; On the other hand, its existence makes the mobile ordering of tank body intrinsic fog, and the air-flow spray is mild, is evenly distributed.And can be without propeller-driven in lower tank body, it is suitable for reading just to make mist go out inner core by self speed, reaches 2-3 times of the height that atomizes when not adding inner core.
3, adopt intermittent spraying.
4, placement of multiple layers stainless (steel) wire in the reactor, plant tissue places on the net.Stationary state has guaranteed the polar effect of plant-growth, growth, and multilayered structure then improved the throughput of unit volume.
Effect of the present invention:
A series of test shows that the atomizing reactor helps extensiveization of the cultivation and the cultivation of plant tissue, cell.
Nutrient mist can spread rapidly in culture apparatus, reaches very soon evenly, even have multilayer net and the highdensity tissue block of late stage of culture or group cultivation to exist, and neither be very big to extending influence of mist.
The upgrowth situation of plant tissue obviously is better than solid medium, and to cultivate tissue growth situation down close with the fluid line layer, even better, (in general, the liquid shallow cultivation is better than solid medium).Long-term cultivation does not have vitrification phenomenon and takes place, and devitrification is arranged, and is better than liquid culture in this.Cultivate pollution-free more than 20 days.
After spraying was cut off, mist can dissipate rapidly---and be attached to other positions of tissue surface or reactor, thereby recover the transparency in the reactor, guaranteed competent illumination.
Reactor is at inner diameter of steel flue, under the constant situation of spraying gun power and other condition, only volume of culture is amplified to 20 liters by 1.5 liters, and the dispersion effect of mist is still fine.Utilize the advantage of this easy amplification and, this kind reactor can be amplified to the technology scale by calculating.
Fig. 1 is the mechanical spraying device.
Fig. 2 is a supersonic atomizer system pie graph.
Fig. 3 is the existing ultrasonic atomization culture systems of Korea S.
Fig. 4 is the existing ultrasonic atomization culture systems of Japan.
Fig. 5 is a tank structure synoptic diagram of the present invention.
The invention will be further described below in conjunction with accompanying drawing.The reactor for nutrient liquid atomizing culture of one kind of plant, it is characterized in that: this reactor comprises the stainless steel kerve 8, atomizing transducing sheet 9, high-frequency oscillating circuits 10, time controller 11, strainer 12, fluorescent lamp 13 of glass tank body 1, Stainless Steel Cloth 2, tissue block 3, inner core 4, water screw 5, electromagnetic driver 6, top cover 7, strap clamp cover, wherein, and electromagnetic driver 6 is arranged on the central authorities of top cover 7; The driving part of electromagnetic driver 6 places outside the jar, and follower is sealed in the jar, is added with water screw on the driven shaft; Top cover 7 is arranged on the glass tank body 1; Placement of multiple layers stainless (steel) wire 2 in the glass tank body 1, plant tissue piece 3 places on the net, glass tank body 1 is arranged on the stainless steel kerve 8, with flange connection, central inner core 4 is arranged in the glass tank body 1 between stainless steel kerve 8 and the glass tank body 1, stainless steel kerve 8 bottoms, its with the transducing sheet of propeller and bottom point-blank, there is a circular hole in the central authorities of stainless steel kerve 8, and atomizing transducing sheet 9 is outside jar.Be fixed on this hole, and strict seal; Stainless steel kerve 8 has chuck; High-frequency oscillating circuits 10 is connected with atomizing transducing sheet 9, and time controller 11 is connected with high-frequency oscillating circuits, and strainer 12 is connected with the inlet mouth of kerve 8, and fluorescent lamp 13 is arranged on outside the glass tank body 1.
Claims (1)
1, the reactor for nutrient liquid atomizing culture of a kind of plant, it is characterized in that: this reactor comprises: glass tank body 1, Stainless Steel Cloth 2, tissue block 3, inner core 4, water screw 5, electromagnetic driver 6, top cover 7, the stainless steel kerve 8 that brings cover, atomizing transducing sheet 9, high-frequency oscillating circuits 10, time controller 11, strainer 12, fluorescent lamp 13, wherein, and electromagnetic driver 6 is arranged on the central authorities of top cover 7; The driving part of electromagnetic driver 6 places outside the jar, and follower is sealed in the jar, is added with water screw on the driven shaft; Top cover 7 is arranged on the glass tank body 1; Placement of multiple layers stainless (steel) wire 2 in the glass tank body 1, plant tissue piece 3 places on the net; Glass tank body 1 is arranged on the stainless steel kerve 8, between stainless steel kerve 8 and the glass tank body 1 with flange connection; Central authorities' inner core 4 is arranged in the glass tank body 1, stainless steel kerve 8 bottoms, its with the transducing sheet of propeller and bottom point-blank, there is a circular hole in the central authorities of stainless steel kerve 8, atomize transducing sheet 9 outside jar, are fixed on this hole, and strict seal; Stainless steel kerve 8 has chuck; High-frequency oscillating circuits 10 is connected with atomizing transducing sheet 9, and time controller 11 is connected with high-frequency oscillating circuits, and strainer 12 is connected with the inlet mouth of kerve 8, and fluorescent lamp 13 is arranged on outside the glass tank body 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96105058A CN1051575C (en) | 1996-05-15 | 1996-05-15 | Reactor for nutrient liquid atomizing culture of plant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96105058A CN1051575C (en) | 1996-05-15 | 1996-05-15 | Reactor for nutrient liquid atomizing culture of plant |
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| CN1142534A true CN1142534A (en) | 1997-02-12 |
| CN1051575C CN1051575C (en) | 2000-04-19 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN96105058A Expired - Fee Related CN1051575C (en) | 1996-05-15 | 1996-05-15 | Reactor for nutrient liquid atomizing culture of plant |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1063224C (en) * | 1998-06-16 | 2001-03-14 | 中国科学院化工冶金研究所 | Airlift cyclic immersion lighting plant cell tissue organ culture method and culture reactor |
| CN100464632C (en) * | 2006-12-07 | 2009-03-04 | 章永泰 | Air culture type plant sugar-free tissue culture device |
| CN101100641B (en) * | 2007-06-13 | 2010-12-29 | 湖南省林产化工工程重点实验室 | Curtain-shape anchoring culture bioreactor and method for culturing plant cell, tissue or organ using the same |
| CN102150617A (en) * | 2011-01-05 | 2011-08-17 | 苏晖阳 | Automatic plant tissue culturing machine |
| CN105230468A (en) * | 2015-11-03 | 2016-01-13 | 北京农业生物技术研究中心 | System and method for producing detoxified lily bulb seed balls in large scale |
| CN105594578A (en) * | 2016-03-15 | 2016-05-25 | 吉林大学 | Domestic step type aeroponic culture device |
| CN107827308A (en) * | 2017-09-27 | 2018-03-23 | 徐州工程学院 | A kind of trace spray irrigation |
| CN110331094A (en) * | 2019-05-15 | 2019-10-15 | 象山罗雅电子科技有限公司 | A kind of plant cell reactor |
| CN111565562A (en) * | 2017-10-13 | 2020-08-21 | 莱图斯种植有限公司 | Aeroponic culture equipment |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6487079A (en) * | 1987-09-28 | 1989-03-31 | Kiyosaki Tekkosho Kk | Welding forming method for case body |
| JPH01187079A (en) * | 1988-01-20 | 1989-07-26 | Toshiba Corp | System for plant culture |
| CN2080522U (en) * | 1990-12-24 | 1991-07-10 | 杭州大学 | Vertical sheet cell incubator |
| JPH05308952A (en) * | 1992-05-06 | 1993-11-22 | Kanebo Ltd | Bioreactor |
-
1996
- 1996-05-15 CN CN96105058A patent/CN1051575C/en not_active Expired - Fee Related
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1063224C (en) * | 1998-06-16 | 2001-03-14 | 中国科学院化工冶金研究所 | Airlift cyclic immersion lighting plant cell tissue organ culture method and culture reactor |
| CN100464632C (en) * | 2006-12-07 | 2009-03-04 | 章永泰 | Air culture type plant sugar-free tissue culture device |
| CN101100641B (en) * | 2007-06-13 | 2010-12-29 | 湖南省林产化工工程重点实验室 | Curtain-shape anchoring culture bioreactor and method for culturing plant cell, tissue or organ using the same |
| CN102150617A (en) * | 2011-01-05 | 2011-08-17 | 苏晖阳 | Automatic plant tissue culturing machine |
| CN102150617B (en) * | 2011-01-05 | 2012-09-05 | 苏晖阳 | Automatic plant tissue culturing machine |
| CN105230468A (en) * | 2015-11-03 | 2016-01-13 | 北京农业生物技术研究中心 | System and method for producing detoxified lily bulb seed balls in large scale |
| CN105230468B (en) * | 2015-11-03 | 2017-11-14 | 北京农业生物技术研究中心 | A kind of system and method for large-scale production lily detoxification seed ball |
| CN105594578A (en) * | 2016-03-15 | 2016-05-25 | 吉林大学 | Domestic step type aeroponic culture device |
| CN107827308A (en) * | 2017-09-27 | 2018-03-23 | 徐州工程学院 | A kind of trace spray irrigation |
| CN111565562A (en) * | 2017-10-13 | 2020-08-21 | 莱图斯种植有限公司 | Aeroponic culture equipment |
| CN110331094A (en) * | 2019-05-15 | 2019-10-15 | 象山罗雅电子科技有限公司 | A kind of plant cell reactor |
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| Publication number | Publication date |
|---|---|
| CN1051575C (en) | 2000-04-19 |
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According to article 9 of the patent law and article 12 of the detailed rules for the implementation of the patent law: 96105058.6 of the invention patents in this issue as a notice of authorization, and at the same time corresponding to the 96211808.7 utility model patent to be given up, and in the 16 volume of the 16 issue of the new type of communique on the patent right to abandon the announcement. |
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