CN105230468B - A kind of system and method for large-scale production lily detoxification seed ball - Google Patents

A kind of system and method for large-scale production lily detoxification seed ball Download PDF

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Publication number
CN105230468B
CN105230468B CN201510736398.XA CN201510736398A CN105230468B CN 105230468 B CN105230468 B CN 105230468B CN 201510736398 A CN201510736398 A CN 201510736398A CN 105230468 B CN105230468 B CN 105230468B
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China
Prior art keywords
nutrient solution
valve
points
lily
pipeline
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Expired - Fee Related
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CN201510736398.XA
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CN105230468A (en
Inventor
杜运鹏
张秀海
陈绪清
王璐
邢礼军
张倩
何京京
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BEIJING AGRICULTURAL BIOLOGICAL TECHNOLOGY Research CENTRE
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BEIJING AGRICULTURAL BIOLOGICAL TECHNOLOGY Research CENTRE
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • A01G31/02Special apparatus therefor
    • A01G31/06Hydroponic culture on racks or in stacked containers
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • A01G31/02Special apparatus therefor
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/005Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • A01G2031/006Soilless cultivation, e.g. hydroponics with means for recycling the nutritive solution
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
    • Y02P60/21Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures

Abstract

The invention discloses a kind of system of large-scale production lily detoxification seed ball, including nutrient solution return tank (1), nutrient solution holding vessel (2) and the atomization incubator (3) being sequentially connected;The atomization incubator (3) is connected by the first pipeline (10) with the entrance of the nutrient solution return tank (1), and the nutrient solution return tank (1) provides device with pressure-air and is connected.The invention also discloses the method for the system production lily detoxification seed ball using the large-scale production lily detoxification seed ball, has the advantages of transformation period is short, pollution rate is low, cost is low, easy to operate.

Description

A kind of system and method for large-scale production lily detoxification seed ball
Technical field
The present invention relates to biological technical field, system and side more particularly to a kind of large-scale production lily detoxification seed ball Method.
Background technology
Lily is the perennial herb bulbous plant of Liliaceae lilium, originates in China, be mainly distributed on east Asia, The Northern Hemisphere Temperate Region in China such as Europe, North America, the whole world has had found at least 120 kinds, wherein 55 kinds originate in China.In recent years more There are much caused new varieties, such as Lilium Pollyanna, Fragrant Lily, fiery lily by artificial hybridization.Bulb contains abundant starch, It is edible, also make medicinal.Lily eats dry use raw.Lily is Chinese tradition outlet special product.
Lily is all the world's famous flower liked by people from ancient times to the present, and lily appearance is graceful, and blade is verdant graceful, stem booth Booth is slim and graceful, is rare cut-flower rising star, Chinese lily have the fine family of life-long happiness and perfect harmony, greatest love of all connotation, have deep The meaning of blessing.
The existing propagation method of lily mainly has scale lining, seed ball breeding and bulbil to breed 3 kinds in production, has numerous It is slow to grow efficiency, balling ratio is relatively low or clove takes root negligible amounts, breeding coefficient is low, and the shortcomings that easily limited by natural conditions, With the development of biotechnology, tissue culture technique improves reproductive efficiency to a certain extent, but still with complex operation, into The shortcomings of this input is big, pollution rate.The high-volume demand in production process can not be met.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of transformation period is short, pollution rate is low, cost is low, easy to operate The system of large-scale production lily detoxification seed ball and the method using the system production lily detoxification seed ball.
A kind of system of large-scale production lily detoxification seed ball, including the nutrient solution return tank, the nutrient solution that are sequentially connected store up Deposit tank and atomization incubator;The atomization incubator is connected by the first pipeline with the entrance of the nutrient solution return tank, described Nutrient solution return tank provides device with pressure-air and is connected.
The system of large-scale production lily detoxification seed ball of the present invention, wherein, set on the nutrient solution return tank There is float-controlled valve, the first valve is provided between the nutrient solution return tank and the pressure-air provide device, described Nutrient solution filter and the second valve are disposed with pipeline between nutrient solution return tank and the nutrient solution holding vessel, It is connected between the nutrient solution holding vessel and the atomization incubator by the second pipeline, the is provided with second pipeline Three valves, the 4th valve is provided with first pipeline.
The system of large-scale production lily detoxification seed ball of the present invention, wherein, first valve is air-powered electromagnetic Valve, second valve and the 4th valve are check valve, and the 3rd valve is aqueous phase magnetic valve.
The system of large-scale production lily detoxification seed ball of the present invention, wherein, the atomization incubator includes support body With top plate, bottom plate, front side wall, rear wall, left side wall and the right side wall being fixed thereon, include training in the atomization incubator Support frame, the culturing rack is made up of parallel multiple object placement nets up and down, be between multiple object placement nets it is detachably connected, The bottom of the top plate is provided with least one water inlet pipe, and multiple atomizers are fixedly connected with the water inlet pipe, the multiple Atomizer is evenly distributed on the bottom of the top plate, and the water inlet pipe is connected with second pipeline;
In the bottom of the culturing rack, the top of the bottom plate is provided with water collection sheet, and the water collection sheet is rectangle, and edge The center line of the rectangular length direction is that axis flips up the cell body to form that cross section is V-shaped, and the water collection sheet inclines The bottom of the culturing rack is tiltedly fixed on, the cell body bottom of the relatively low side is provided with semicircular water collector, the collection The bottom of hydrophone is connected with outlet pipe, and the outlet pipe is connected with first pipeline.
The system of large-scale production lily detoxification seed ball of the present invention, wherein, center line and the level of the water collection sheet Angle between face is 10~15 °;The section of the bottom plate is right angled triangle, is arranged on the bottom of the water collection sheet, described The bottom surface of bottom plate is horizontal plane, and upper surface tilts, and incline direction is consistent with the incline direction of the water collection sheet;In the object placement net On be provided with cultivation matrix, thickness is 2~3cm, and the cultivation matrix is haydite;
The top plate, the bottom plate, the front side wall, the rear wall, the left side wall and the right side wall are by saturating Bright material is made, and is to be flexibly connected between the front side wall and the support body;There is indigo plant in the external stability of the atomization incubator Purple light source, the blue-violet light source is the LED for sending blue light, in mesh arrangement;
Second pipeline is connected through the bottom plate and the water collection sheet with the water inlet pipe, and second pipeline point It is not welded and fixed with the bottom plate and the water collection sheet.
Using the method for the system production lily detoxification seed ball of large-scale production lily detoxification seed ball of the present invention, bag Include following steps:
The atomization culture being put into after lily bud scale is cleaned up in the system of large-scale production lily detoxification seed ball Cultivated on cultivation matrix in case, 13-15 days, lily bud scale base portion can sprout adventitious bud, can obtain lily within 45 days Detoxification seed ball;
The incubation uses spray process, carries out the spray process process every time and comprises the following steps:
Step 1: nutrient solution filtering and storage of higher pressures:First valve is opened, and now float-controlled valve and the 4th valve are because of high pressure gas Body is acted on and closed, and the 4MPa gases at high pressure that device offer is provided by gases at high pressure push away the nutrient solution in nutrient solution return tank Go out, by nutrient solution filter and the second valve, stored into nutrient solution holding vessel;During this, the 3rd valve, which is in, to close Closed state, this process duration are 5 minutes, i.e. first valve opening after 5 minutes, 5 minutes, close, and cuts by the first valve Disconnected gases at high pressure, meanwhile, the nutrient solution return tank is reduced to rapidly normal atmosphere (An), and the float-controlled valve declines, the culture Liquid return tank communicates with ambient atmosphere;
Step 2: atomization incubator spraying:Frequency and time according to setting, the 3rd valve opening, the culture The nutrient solution of liquid holding vessel mesohigh storage enters the atomizer of atomization incubator by the 3rd valve, makes culture liquid mist Change, this process duration after 5 minutes, 5 minutes, close by the 3rd valve;
Step 3: the nutrient solution backflow in atomization incubator:It is atomized after the completion of incubator spraying, nutrient solution is by bottom V-shaped tank is flowed into water collector, and the 4th valve opening, nutrient solution enters the nutrient solution return tank, this process duration 15 minutes.
The method of production lily detoxification seed ball of the present invention, the incubation include 4 stages:
First stage:Time is the 1st day~the 6th day, daily 0 point, 4 points, 7 points, 11 points, 15 points and 20 points totally 6 when Between point, carry out a spray process respectively at each time point, the nutrient solution used is MS+0.2mg/L GA3+2mg/L6-BA+ 1000mg/L carbendazim;
Second stage:Time is the 7th day~the 20th day, daily 0 point, 5 points, 9 points, 13 points and 18 points totally 5 time points, Spray process twice is carried out respectively at each time point, and the nutrient solution used is MS+0.05mg/L TDZ+1.8mg/L6-BA+ 200mg/L paclobutrazols;
Phase III:Time is the 21st day~the 35th day, daily 3 points, 7 points, 13 points and 20 points totally 4 time points, Each time point carries out spray process twice respectively, and the nutrient solution used is MS+0.15mg/L NAA+0.8mg/L6-BA+ 300mg/L carbendazim;
Fourth stage:Time is the 36th day~the 45th day, daily 0 point, 4 points, 7 points, 10 points, 15 points and 19 points totally 6 At time point, carry out a spray process respectively at each time point, the nutrient solution used is MS+0.4mg/L TDZ+1.2mg/L 6-BA+50mg/L paclobutrazols.
The system difference from prior art of large-scale production lily detoxification seed ball of the present invention is:
The system of large-scale production lily detoxification seed ball of the present invention has the following advantages that:
1) incubation of the invention is operated under open environment, is not limited by environmental condition, easy to operate;
2) traditional incubation workload is big, low reproduction rate, and tissue culture procedures workload is big, and pollution rate is high, unfavorable In large-scale production, and workload of the present invention is small, workable and can realize production in enormous quantities, and open culture is not present again Pollution problem;
3) present invention makes the nutrient of nutrient solution efficiently be utilized using spray process method, open culture, preferably Promote the growth of seedling, do not need many matrix in incubation, floor space is small;
The method of the invention is with short production cycle, substantially increases efficiency and productivity ratio.
The present invention can also use blue violet light to carry out light filling, be chiefly to facilitate the differentiation of plant cell, and promote plant to stretch Length is formed with young shoot;The structure of the atomization incubator of the present invention is unique design, and pressure-air provides device, nutrient solution backflow Tank, nutrient solution filter, nutrient solution holding vessel and each valve are prior arts, and of the invention focuses on it with spy The atomization incubator of different connected mode and unique design connects, and form large-scale production lily detoxification seed ball of the present invention is System, it is possible to increase the utilization rate of nutrient solution, utilize illumination condition and greenhouse space to greatest extent.And beneficial to operation, manager Just.
Using the system production lily detoxification seed ball of the present invention, point 4 stages are carried out, at different time points according to lily Operational phase residing for scale explant is cultivated using different culture mediums, different spray times and spray mode, numerous Efficiency high is grown, is not polluted, occupation of land is small, significantly reduces working strength.
The system of the large-scale production lily detoxification seed ball of the present invention is described further below in conjunction with the accompanying drawings.
Brief description of the drawings
Fig. 1 is the structural representation of the system of large-scale production lily detoxification seed ball of the present invention;
Fig. 2 is the structural representation that incubator is atomized in the system of large-scale production lily detoxification seed ball of the present invention;
Fig. 3 is the structural representation of water collection sheet in present invention atomization incubator.
Embodiment
Embodiment 1
As shown in FIG. 1 to 3, the system of large-scale production lily detoxification seed ball of the present invention includes the nutrient solution being sequentially connected Return tank 1, nutrient solution holding vessel 2 and atomization incubator 3;It is atomized incubator 3 and passes through the first pipeline 10 and nutrient solution return tank 1 Entrance is connected, and nutrient solution return tank 1 provides device with pressure-air and is connected.
Float-controlled valve 4 is provided with nutrient solution return tank 1, between nutrient solution return tank 1 and pressure-air provide device The first valve 5 is provided with, nutrient solution mistake is disposed with the pipeline between nutrient solution return tank 1 and nutrient solution holding vessel 2 The valve 7 of device 6 and second is filtered, is connected between nutrient solution holding vessel 2 and atomization incubator 3 by the second pipeline 11, in the second pipe The 3rd valve 8 is provided with road 11, the 4th valve 9 is provided with the first pipeline 10.First valve 5 is air-operated solenoid valve, the Two valves 7 and the 4th valve 9 are check valve, and the 3rd valve 8 is aqueous phase magnetic valve.
Atomization incubator 3 includes support body and the top plate, bottom plate, front side wall, rear wall, left side wall and the right side that are fixed thereon Wall, culturing rack 301 is included in atomization incubator 3, culturing rack 301 is made up of multiple object placement nets 302 parallel up and down, multiple to put It is detachably connected between thing net 302, at least one water inlet pipe 303, multiple atomizers 304 is provided with the bottom of top plate It is fixedly connected with water inlet pipe 303, multiple atomizers 304 are evenly distributed on the bottom of top plate, the pipeline 11 of water inlet pipe 303 and second It is connected;The quantity of water inlet pipe 303 and atomizer 304 so that equably as standard, arbitrary quantity can be set by irrigating plant, Such as 8,10,20 etc., when plant is more, can also be respectively provided with the top of each layer commodity net 302 into water Pipe 303 and atomizer 304, all water inlet pipes 303 are connected with the second pipeline 11 respectively by pipeline.
In the bottom of culturing rack 301, the top of bottom plate is provided with water collection sheet 305, and water collection sheet 305 is rectangle, and along length The center line of square length direction is that axis flips up to form the cell body that cross section is V-shaped and (could also say that two block length sides Shape plate body, a side of the length direction of the two is connected, at an acute angle between the plate face of two pieces of rectangular plate bodies), water collection sheet 305 inclines The bottom of culturing rack 301 is tiltedly fixed on, the cell body bottom of relatively low side is provided with semicircular water collector 306, water collector 306 Bottom be connected with outlet pipe 307, outlet pipe 307 is connected with the first pipeline 10.
Angle between the center line and horizontal plane of water collection sheet 305 is 10~15 °, so that the nutrient solution left is more smoothly more square Just it is gathered in water collector 306;The section of bottom plate is right angled triangle, is arranged on the bottom of water collection sheet 305, the bottom surface of bottom plate For horizontal plane, upper surface tilts, and incline direction is consistent with the incline direction of water collection sheet 305;Cultivation is provided with object placement net 302 Matrix, thickness are 2~3cm, and cultivation matrix is haydite;
Top plate, bottom plate, front side wall, rear wall, left side wall and right side wall are made of clear material, front side wall and support body it Between for be flexibly connected;There is blue-violet light source in the external stability of atomization incubator 3, blue-violet light source is the LED for sending blue light Light bar, in mesh arrangement;
Second pipeline 11 is connected through bottom plate and water collection sheet 305 with water inlet pipe 303, and the second pipeline 11 respectively with bottom plate and Water collection sheet 305 is welded and fixed, between to be tightly connected, nutrient solution will not reveal, and facilitate hiding for pipeline, more attractive.
Using the method for the system production lily detoxification seed ball of the large-scale production lily detoxification seed ball of the present invention, including such as Lower step:
The atomization culture being put into after lily bud scale is cleaned up in the system of large-scale production lily detoxification seed ball Cultivated on cultivation matrix in case 3,13-15 days, lily bud scale base portion can sprout adventitious bud, can obtain lily within 45 days Detoxification seed ball;
Incubation uses spray process, carries out spray process process every time and comprises the following steps:
Step 1: nutrient solution filtering and storage of higher pressures:First valve 5 is opened, and now the valve 9 of float-controlled valve 4 and the 4th is because of height Body of calming the anger is acted on and closed, and the 4MPa gases at high pressure of device offer are provided by the culture in nutrient solution return tank 1 by gases at high pressure Liquid is released, and by the valve 7 of nutrient solution filter 6 and second, is stored into nutrient solution holding vessel 2;During this, the 3rd valve 8 are closed, and this process duration is 5 minutes, i.e. after the first valve 5 is opened 5 minutes, 5 minutes, the first valve 5 closes Close, cutoff high gas, meanwhile, nutrient solution return tank 1 is reduced to rapidly normal atmosphere (An), and float-controlled valve 4 declines, nutrient solution backflow Tank 1 communicates with ambient atmosphere;
Step 2: atomization incubator 3 is sprayed:Frequency and time according to setting, the 3rd valve 8 are opened, nutrient solution storage The nutrient solution of the mesohigh of tank 2 storage enters the atomizer 304 of atomization incubator 3 by the 3rd valve 8, is atomized nutrient solution, After this process duration 5 minutes, 5 minutes, the 3rd valve 8 is closed;
Step 3: the nutrient solution backflow in atomization incubator 3:It is atomized after the completion of the spraying of incubator 3, nutrient solution is by bottom V-shaped tank flow into water collector 306, the 4th valve 9 is opened, and nutrient solution enters nutrient solution return tank 1, this process persistently when Between 15 minutes.
Wherein, incubation includes 4 stages:
First stage:Time is the 1st day~the 6th day, daily 0 point, 4 points, 7 points, 11 points, 15 points and 20 points totally 6 when Between point, carry out a spray process respectively at each time point, the nutrient solution used is MS+0.2mg/L GA3+2mg/L6-BA+ 1000mg/L carbendazim;
Second stage:Time is the 7th day~the 20th day, daily 0 point, 5 points, 9 points, 13 points and 18 points totally 5 time points, Spray process twice is carried out respectively at each time point, and the nutrient solution used is MS+0.05mg/L TDZ+1.8mg/L6-BA+ 200mg/L paclobutrazols;
Phase III:Time is the 21st day~the 35th day, daily 3 points, 7 points, 13 points and 20 points totally 4 time points, Each time point carries out spray process twice respectively, and the nutrient solution used is MS+0.15mg/L NAA+0.8mg/L6-BA+ 300mg/L carbendazim;
Fourth stage:Time is the 36th day~the 45th day, daily 0 point, 4 points, 7 points, 10 points, 15 points and 19 points totally 6 At time point, carry out a spray process respectively at each time point, the nutrient solution used is MS+0.4mg/L TDZ+1.2mg/L 6-BA+50mg/L paclobutrazols.
Embodiment above is only that the preferred embodiment of the present invention is described, and not the scope of the present invention is entered Row limits, and on the premise of design spirit of the present invention is not departed from, those of ordinary skill in the art make to technical scheme The various modifications gone out and improvement, it all should fall into the protection domain of claims of the present invention determination.

Claims (5)

  1. A kind of 1. system of large-scale production lily detoxification seed ball, it is characterised in that:Including the nutrient solution return tank being sequentially connected (1), nutrient solution holding vessel (2) and atomization incubator (3);The atomization incubator (3) passes through the first pipeline (10) and the training The entrance of nutrient solution return tank (1) is connected, and the nutrient solution return tank (1) provides device with pressure-air and is connected;
    The atomization incubator (3) includes support body and the top plate, bottom plate, front side wall, rear wall, left side wall and the right side that are fixed thereon Side wall, culturing rack (301) is included in the atomization incubator (3), the culturing rack (301) is by multiple gloves parallel up and down Net (302) is formed, and is detachably connected between multiple object placement nets (302), is provided with least in the bottom of the top plate One water inlet pipe (303), multiple atomizers (304) are fixedly connected with the water inlet pipe (303), multiple atomizers (304) bottom of the top plate is evenly distributed on, the water inlet pipe (303) is connected with the second pipeline (11);
    In the bottom of the culturing rack (301), the top of the bottom plate is provided with water collection sheet (305), and the water collection sheet (305) is Rectangle, and be that axis flips up to form the cell body that cross section is V-shaped, institute along the center line of the rectangular length direction State water collection sheet (305) and tilt the bottom for being fixed on the culturing rack (301), the cell body bottom of relatively low side is provided with semicircle Water collector (306), the bottom of the water collector (306) is connected with outlet pipe (307), the outlet pipe (307) and described the One pipeline (10) is connected;
    Angle between the center line and horizontal plane of the water collection sheet (305) is 10~15 °;The section of the bottom plate is right angle trigonometry Shape, the bottom of the water collection sheet (305) is arranged on, the bottom surface of the bottom plate is horizontal plane, and upper surface tilts, incline direction and institute The incline direction for stating water collection sheet (305) is consistent;It is provided with cultivation matrix on the object placement net (302), thickness is 2~3cm, institute It is haydite to state cultivation matrix;
    The top plate, the bottom plate, the front side wall, the rear wall, the left side wall and the right side wall are by transparent material Material is made, and is to be flexibly connected between the front side wall and the support body;There is royal purple in the external stability of the atomization incubator (3) Color light source, the blue-violet light source is the LED for sending blue light, in mesh arrangement;
    Second pipeline (11) is connected through the bottom plate and the water collection sheet (305) with the water inlet pipe (303), and described Second pipeline (11) is welded and fixed with the bottom plate and the water collection sheet (305) respectively.
  2. 2. the system of large-scale production lily detoxification seed ball according to claim 1, it is characterised in that:The nutrient solution returns Float-controlled valve (4) is provided with stream tank (1), is set between the nutrient solution return tank (1) and the pressure-air provide device There is the first valve (5), set gradually on the pipeline between the nutrient solution return tank (1) and the nutrient solution holding vessel (2) There are nutrient solution filter (6) and the second valve (7), lead between the nutrient solution holding vessel (2) and the atomization incubator (3) Cross the second pipeline (11) to be connected, the 3rd valve (8) is provided with second pipeline (11), on first pipeline (10) It is provided with the 4th valve (9).
  3. 3. the system of large-scale production lily detoxification seed ball according to claim 2, it is characterised in that:First valve (5) it is air-operated solenoid valve, second valve (7) and the 4th valve (9) are check valve, and the 3rd valve (8) is water Phase magnetic valve.
  4. 4. the method for the system production lily detoxification seed ball using the large-scale production lily detoxification seed ball described in claim 3, It is characterized in that:Comprise the following steps:
    The atomization incubator (3) being put into after lily bud scale is cleaned up in the system of large-scale production lily detoxification seed ball In cultivation matrix on cultivated, 13-15 days, lily bud scale base portion can sprout adventitious bud, can obtain lily within 45 days and take off Malicious seed ball;
    The incubation uses spray process, carries out the spray process process every time and comprises the following steps:
    Step 1: nutrient solution filtering and storage of higher pressures:First valve (5) is opened, now float-controlled valve (4) and the 4th valve (9) because Gases at high pressure are acted on and closed, and the 4MPa gases at high pressure of device offer are provided by nutrient solution return tank (1) by gases at high pressure Nutrient solution is released, and by nutrient solution filter (6) and the second valve (7), is stored into nutrient solution holding vessel (2);This process In, the 3rd valve (8) is closed, and this process duration is 5 minutes, i.e. the first valve (5) unlatching 5 minutes, and 5 After minute, the first valve (5) is closed, cutoff high gas, meanwhile, the nutrient solution return tank (1) is reduced to rapidly normal big Air pressure, the float-controlled valve (4) decline, and the nutrient solution return tank (1) communicates with ambient atmosphere;
    Step 2: atomization incubator (3) spraying:Frequency and time according to setting, the 3rd valve (8) are opened, the training The nutrient solution of nutrient solution holding vessel (2) mesohigh storage enters the atomizer of atomization incubator (3) by the 3rd valve (8) (304), it is atomized nutrient solution, this process duration after 5 minutes, 5 minutes, close by the 3rd valve (8);
    Step 3: the nutrient solution backflow in atomization incubator (3):It is atomized after the completion of incubator (3) spraying, nutrient solution is by bottom V-shaped cell body flow into water collector (306), the 4th valve (9) is opened, and nutrient solution enters the nutrient solution return tank (1), this Process duration 15 minutes.
  5. 5. using the method for the production lily detoxification seed ball described in claim 4, it is characterised in that:The incubation includes 4 The individual stage:
    First stage:Time is the 1st day~the 6th day, daily 0 point, 4 points, 7 points, 11 points, 15 points and 20 points totally 6 time points, Carry out a spray process respectively at each time point, the nutrient solution used is MS+0.2mg/L GA3+2mg/L 6-BA+ 1000mg/L carbendazim;
    Second stage:Time is the 7th day~the 20th day, daily 0 point, 5 points, 9 points, 13 points and 18 points totally 5 time points, every Individual time point carries out spray process twice respectively, and the nutrient solution used is MS+0.05mg/L TDZ+1.8mg/L 6-BA+ 200mg/L paclobutrazols;
    Phase III:Time is the 21st day~the 35th day, daily 3 points, 7 points, 13 points and 20 points totally 4 time points, each Time point carries out spray process twice respectively, and the nutrient solution used is MS+0.15mg/L NAA+0.8mg/L 6-BA+300mg/L Carbendazim;
    Fourth stage:Time is the 36th day~the 45th day, daily 0 point, 4 points, 7 points, 10 points, 15 points and 19 points totally 6 time Point, carry out a spray process respectively at each time point, the nutrient solution used is MS+0.4mg/L TDZ+1.2mg/L 6-BA + 50mg/L paclobutrazols.
CN201510736398.XA 2015-11-03 2015-11-03 A kind of system and method for large-scale production lily detoxification seed ball Expired - Fee Related CN105230468B (en)

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CN105230468A CN105230468A (en) 2016-01-13
CN105230468B true CN105230468B (en) 2017-11-14

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