CN104186317B - The tissue culture and rapid propagation method of Yunnan crape myrtle - Google Patents
The tissue culture and rapid propagation method of Yunnan crape myrtle Download PDFInfo
- Publication number
- CN104186317B CN104186317B CN201410394862.7A CN201410394862A CN104186317B CN 104186317 B CN104186317 B CN 104186317B CN 201410394862 A CN201410394862 A CN 201410394862A CN 104186317 B CN104186317 B CN 104186317B
- Authority
- CN
- China
- Prior art keywords
- seed
- yunnan
- crape myrtle
- root
- seedling
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Mushroom Cultivation (AREA)
- Cultivation Of Plants (AREA)
Abstract
The present invention relates to the tissue culture and rapid propagation method of Yunnan crape myrtle, in Yunnan crape myrtle seed maturity season, to gather lignification but uncracked fruit, under 4 DEG C of conditions, store 2 weeks, then sterilizing is carried out to whole fruit, cut fruit open, take out seed, remove seed wing, be inoculated in the 1/2MS minimal medium containing 0.5g/L active carbon, light culture 1-2 week cultivates under then forwarding light to until seed germination; Sprouting seedling highly more than 2cm is inoculated on inducing clumping bud medium, cultivates 5-6 week, obtain a large amount of Multiple Buds, Multiple Buds is inoculated on root media, cultivates 6-8 week, until all aseptic seedling are all taken root, then hardening is carried out to seedling of taking root, transplant bottle outlet.The present invention starts with from the breeding of Yunnan crape myrtle, utilizes the method for tissue cultures to solve its seed germination difficulty, problem that reproduction coefficient is lower under field conditions (factors), for Yunnan crape myrtle Germ-plasma resources protection and carry out more deep research and lay the foundation.
Description
Technical field
The present invention relates to plant tissue culture technique, specifically, relate to the tissue culture and rapid propagation method of Yunnan crape myrtle.
Background technology
Yunnan crape myrtle (LagerstroemiaintermediaKoehne) is Lythraceae (Lythraceae), and the evergreen megaphanerophyte of Lagerstroemia is state three level protecting plant.There are plant of Lagerstroemia about 55 kinds in the current whole world, and be mainly distributed in east Asia, the southeast and Australia are northern, China originates in 18 kinds of plant of Lagerstroemias, after from Southeast Asia introduction cultivation 3 kinds, totally 21 kinds.Comparatively speaking, the bright-coloured beauty of pattern, the florescence is long, and the life-span is long, also has contamination resistance in addition strong, adapts to the advantages such as urban environment, therefore very popular, is widely used in afforestation for plant of Lagerstroemia and other flowers and trees.
Plant of Lagerstroemia common in current city has crape myrtle, banaba, Fujian crape myrtle, Guizhou Province, river crape myrtle etc., Yunnan crape myrtle is the important kind of of plant of Lagerstroemia, be special product of China seeds, be mainly distributed in Simao, Yunnan and billows deep blue, mix and be born in the shaw of 1800 meters.Compared with other plant of Lagerstroemias, Yunnan crape myrtle not only has beautiful very large flower, and its florescence early, and namely May enters full-bloom stage, and the crape myrtle of Beijing area has just just sprouted May, therefore it can as the important parent of plant of Lagerstroemia early blossoming breeding research; Another important feature of Yunnan crape myrtle is it is evergreen megaphanerophyte, is the evergreen kind of only one in plant of Lagerstroemia, and therefore the improvement of introducing to plant of Lagerstroemia fancy points of Yunnan crape myrtle germ plasm resource has great significance.
Crape myrtle plant is tall and big tall and straight in Yunnan, and blade ovalize or square round shape ellipse, blade is equally large and beautiful with flower, has ornamental value, and be rare early blossoming seeds and evergreen species, and plant is tall and big, plant type is tall and straight.Though natural conditions descend fruit power strong, natural renovation is bad, and sylvan life treelet is rare, and this may be because the hard and seed wing of the seed seed coat of Yunnan crape myrtle causes more greatly, is therefore difficult to obtain a large amount of seedling.Tissue culture technique is a kind of common plant fast propagation means, mostly can realize numerous soon by the means of group training, thus make preserving seed and production popularization become possibility for the kind of breeding difficulty under field conditions (factors).
Because Yunnan crape myrtle is China's endemic tree, only in ground distributions such as Simao, Yunnan billows deep blues, the research at present about Yunnan crape myrtle is also in space state.
Summary of the invention
The object of this invention is to provide the tissue culture and rapid propagation method of Yunnan crape myrtle.
In order to realize the object of the invention, the tissue culture and rapid propagation method of Yunnan provided by the invention crape myrtle, in Yunnan crape myrtle seed maturity season, gather lignification but uncracked fruit, 2 weeks are stored under 4 DEG C of conditions, then sterilizing is carried out to whole fruit, cut fruit open, take out seed, remove seed wing, be inoculated in the 1/2MS minimal medium containing 0.5g/L active carbon, light culture 1-2 week cultivates under then forwarding light to until seed germination; Sprouting seedling highly more than 2cm is inoculated on inducing clumping bud medium, cultivates 5-6 week, obtain a large amount of Multiple Buds, Multiple Buds is inoculated on root media, cultivates 6-8 week, until all aseptic seedling are all taken root, then hardening is carried out to seedling of taking root, transplant bottle outlet.
Particularly, said method comprising the steps of:
(1) 11-12 month, Yunnan crape myrtle seed maturity season, to gather lignification but uncracked fruit, in 4 DEG C of refrigerators, store 2 weeks, soak 30min with the clean spirit of washing of dilution 100 times, grind off pericarp with cleaning ball, then under flowing water, more than 2h is rinsed, with 75% alcohol sterilizing 1min in superclean bench, then use 4% liquor natrii hypochloritis's sterilizing 30min, with aseptic water washing 5-6 time;
(2) cut the fruit of sterilizing open, take out seed, cut away seed wing with sterile scalpel, be advisable to expose seed, be inoculated in the 1/2MS minimal medium containing 0.5g/L active carbon, about light culture 1-2 week, seed starts to sprout seedling, cultivates under being then transferred to light;
Minimal medium is: 1/2MS+0.5g/L active carbon+30g/L sucrose+6.5g/L agar, pH5.8-6.0; Cultivation temperature 25 ± 2 DEG C, light application time 12-14h/d, intensity of illumination 2000-3000lx;
(3) select highly more than the aseptic seeding seedling of 2cm, cut off root and unnecessary blade by sterile scissors, be inoculated in inducing clumping bud medium, carry out inducing clumping bud;
Inducing clumping bud medium is: WPM+0.5-1.0mg/L6-BA+0.025-0.1mg/LIBA+20g/L sucrose+6.5g/L agar, pH5.2-5.4; Cultivation temperature 25 ± 2 DEG C, light application time 12-14h/d, intensity of illumination 2000-3000lx;
(4), after treating that inducing clumping bud cultivates 5-6 week, select highly more than 3cm, and the Multiple Buds of robust growth is inoculated in root induction medium, carries out root induction;
Root induction medium is: 1/2MS+0.025-0.05mg/L6-BA+0.1-1.0mg/LIBA+0.5g/L active carbon+30g/L sucrose+6.5g/L agar, pH5.8-6.0; Cultivation temperature 25 ± 2 DEG C, light application time 12-14h/d, intensity of illumination 2000-3000lx;
(5) 3-4cm length is reached when aseptic seedling root growth is intact, and when being no less than 5, open sealed membrane and train indoor hardening 1 week in group, then the medium of plantlet in vitro root is washed away with sterile water, transplanting to the sterilized peat composed of rotten mosses and perlite volume ratio is in the mixed-matrix of 3:1, and bagging keeps humidity, and keeps spray water every day 2-3 time, then reduce humidity gradually and increase intensity of illumination, after 2 weeks, after transplanted seedling survives, in greenhouse, carrying out cellar culture.
The present invention is also provided for the minimal medium of Yunnan crape myrtle group training, and described minimal medium is: 1/2MS+0.5g/L active carbon+30g/L sucrose+6.5g/L agar, pH5.8-6.0.
The present invention is also provided for the inducing clumping bud medium of Yunnan crape myrtle group training, and described inducing clumping bud medium is: WPM+0.5-1.0mg/L6-BA+0.025-0.1mg/LIBA+20g/L sucrose+6.5g/L agar, pH5.2-5.4.
The present invention is also provided for the root induction medium of Yunnan crape myrtle group training, and described root induction medium is: 1/2MS+0.025-0.05mg/L6-BA+0.1-1.0mg/LIBA+0.5g/L active carbon+30g/L sucrose+6.5g/L agar, pH5.8-6.0.
The present invention starts with from the breeding of Yunnan crape myrtle, the method of tissue cultures is utilized to solve its problem that seed germination is difficult under field conditions (factors), reproduction coefficient is lower, utilize the inventive method, every seedling from seed can a disposable acquisition 4-7 seedling, for follow-up Yunnan crape myrtle Germ-plasma resources protection and carry out more deep research and lay the foundation.
(1) the present invention provides the group culturation rapid propagating technology of China distinctive plant of Lagerstroemia Yunnan crape myrtle of complete set first.
(2) use the seed of Yunnan crape myrtle as explant, easily obtain, and convenient transport, be suitable for studying it in different place.Utilize the method for tissue cultures, a large amount of Multiple Buds can be obtained in a short time, solve the problem that Yunnan crape myrtle breeds difficulty under field conditions (factors).
(3) the invention provides a kind of simple, economic, efficient tissue culture and rapid propagation method, be conducive to the Germ-plasma resources protection of Yunnan crape myrtle and apply.Also can be generalized to Lagerstroemia other kind of not easily breeding and kinds simultaneously.
Accompanying drawing explanation
Fig. 1 is the ripe but non-dehiscent fruit schematic diagram of Yunnan crape myrtle in the embodiment of the present invention 1.
Fig. 2 is Yunnan crape myrtle aseptic seeding and seed germination schematic diagram in the embodiment of the present invention 1.
Fig. 3 is Yunnan crape myrtle inducing clumping bud schematic diagram in the embodiment of the present invention 1.
Fig. 4 is that in the embodiment of the present invention 1, Yunnan crape myrtle plantlet in vitro is taken root schematic diagram.
Fig. 5 is Yunnan crape myrtle plantlet in vitro root system schematic diagram in the embodiment of the present invention 1.
Fig. 6 is that in the embodiment of the present invention 1, Yunnan crape myrtle plantlet in vitro transplants schematic diagram.
Embodiment
Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.If do not specialize, the conventional means that technological means used in embodiment is well known to those skilled in the art, is raw materials usedly commercial goods.
The tissue culture and rapid propagation method of embodiment 1 Yunnan crape myrtle
1,11-12 month, Yunnan crape myrtle seed maturity season, gather lignification but uncracked fruit (Fig. 1), take back laboratory, low-temperature storage 2 weeks in the refrigerator of 4 DEG C.Soak 30min with the clean spirit of washing of dilution 100 times, grind off pericarp with cleaning ball, then under flowing water, rinse more than 2h, with 75% alcohol sterilizing 1min in superclean bench, with 4% liquor natrii hypochloritis's sterilizing 30min, with aseptic water washing 5-6 time.
(2) cut the fruit of sterilizing open, take out seed, cut away seed wing with sterile scalpel, be advisable to expose seed.Be inoculated in the 1/2MS minimal medium containing 0.5g/L active carbon, about light culture 1-2 week, seed starts to sprout seedling, cultivates (Fig. 2) under being then transferred to light.
Minimal medium is: 1/2MS+0.5g/L active carbon+30g/L sucrose+6.5g/L agar, Medium's PH Value is 5.8-6.0, cultivation temperature 25 ± 2 DEG C, and light application time 12-14h/d, intensity of illumination 2000-3000lx, can obtain the germination rate of 30-40%.
(3) select highly more than the aseptic seeding seedling of 2cm, cut off root and unnecessary blade by sterile scissors, be inoculated in inducing clumping bud medium, carry out inducing clumping bud (Fig. 3).
Inducing clumping bud medium is: WPM+0.5-1.0mg/L6-BA+0.025-0.1mg/LIBA+20g/L sucrose+6.5g/L agar, Medium's PH Value is 5.2-5.4, cultivation temperature 25 ± 2 DEG C, light application time 12-14h/d, intensity of illumination 2000-3000lx, each explant can obtain 3-5 regeneration bud.
(4), after treating that inducing clumping bud cultivates 5-6 week, select highly more than 3cm, and the Multiple Buds of robust growth is inoculated in root induction medium, carries out root induction (Fig. 4).
Root induction medium is: 1/2MS+0.025-0.05mg/L6-BA+0.1-1.0mg/LIBA+0.5g/L active carbon+30g/L sucrose+6.5g/L agar, Medium's PH Value is 5.8-6.0, cultivation temperature 25 ± 2 DEG C, light application time 12-14h/d, intensity of illumination 2000-3000lx, rooting rate reaches 100%.
(5) 3-4cm length is reached when aseptic seedling root growth is intact, and when being no less than 5 (Fig. 5), open sealed membrane and train indoor hardening 1 week in group, then the medium of plantlet in vitro root is washed away with sterile water, transplanting the extremely sterilized peat composed of rotten mosses and perlite volume ratio is (Fig. 6) in the mixed-matrix of 3:1, bagging keeps humidity, and keep spray water every day 2-3 time, then reduce humidity gradually and increase intensity of illumination, can carry out cellar culture in greenhouse after 2 weeks after transplanted seedling survives, transplanting survival rate reaches more than 70%.
The tissue culture and rapid propagation method of embodiment 2 Yunnan crape myrtle
(1) 11-12 month, Yunnan crape myrtle seed maturity season, gather lignification but uncracked fruit, take back laboratory, low-temperature storage 2 weeks in the refrigerator of 4 DEG C.Soak 30min with the clean spirit of washing of dilution 100 times, grind off pericarp with cleaning ball, then under flowing water, rinse more than 2h, with 75% alcohol sterilizing 1min in superclean bench, with 4% liquor natrii hypochloritis's sterilizing 30min, with aseptic water washing 5-6 time.
(2) cut the fruit of sterilizing open, take out seed, cut away seed wing with sterile scalpel, be advisable to expose seed.Be inoculated in the 1/2MS minimal medium containing 0.5g/L active carbon, about light culture 1-2 week, seed starts to sprout seedling, cultivates under being then transferred to light.
Minimal medium is: 1/2MS+0.5g/L active carbon+30g/L sucrose+6.5g/L agar, Medium's PH Value is 5.8-6.0, cultivation temperature 25 ± 2 DEG C, and light application time 12-14h/d, intensity of illumination 2000-3000lx, can obtain the germination rate of 30-40%.
(3) select highly more than the aseptic seeding seedling of 2cm, cut off root and unnecessary blade by sterile scissors, be inoculated in inducing clumping bud medium, carry out inducing clumping bud.
Inducing clumping bud medium is: WPM+0.5-1.0mg/L6-BA+0.025-0.1mg/LIBA+20g/L sucrose+6.5g/L agar, Medium's PH Value is 5.2-5.4, cultivation temperature 25 ± 2 DEG C, light application time 12-14h/d, intensity of illumination 2000-3000lx, each explant can obtain 4-7 regeneration bud.
(4), after treating that inducing clumping bud cultivates 5-6 week, select highly more than 3cm, and the Multiple Buds of robust growth is inoculated in root induction medium, carries out root induction.
Root induction medium is: 1/2MS+0.025-0.05mg/L6-BA+0.1-1.0mg/LIBA+0.5g/L active carbon+30g/L sucrose+6.5g/L agar, Medium's PH Value is 5.8-6.0, cultivation temperature 25 ± 2 DEG C, light application time 12-14h/d, intensity of illumination 2000-3000lx, rooting rate reaches 100%.
(5) 3-4cm length is reached when aseptic seedling root growth is intact, and when being no less than 5, open sealed membrane and train indoor hardening 1 week in group, then wash away the medium of plantlet in vitro root with sterile water, transplanting to the sterilized peat composed of rotten mosses and perlite volume ratio is in the mixed-matrix of 3:1, and bagging keeps humidity, and keep spray water every day 2-3 time, then reduce humidity gradually and increase intensity of illumination, can carry out cellar culture in greenhouse after 2 weeks after transplanted seedling survives, transplanting survival rate reaches more than 70%.
Although above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.
Claims (1)
1. the tissue culture and rapid propagation method of Yunnan crape myrtle, it is characterized in that, in Yunnan crape myrtle seed maturity season, gather lignification but uncracked fruit, 2 weeks are stored under 4 DEG C of conditions, then sterilizing is carried out to whole fruit, cut fruit open, take out seed, remove seed wing, be inoculated in the 1/2MS minimal medium containing 0.5g/L active carbon, light culture 1-2 week cultivates under then forwarding light to until seed germination; Sprouting seedling highly more than 2cm is inoculated on inducing clumping bud medium, cultivates 5-6 week, obtain a large amount of Multiple Buds, Multiple Buds is inoculated on root induction medium, cultivates 6-8 week, until all aseptic seedling are all taken root, then hardening is carried out to seedling of taking root, transplant bottle outlet;
Concrete grammar comprises the following steps:
(1) 11-12 month, Yunnan crape myrtle seed maturity season, to gather lignification but uncracked fruit, in 4 DEG C of refrigerators, store 2 weeks, soak 30min with the clean spirit of washing of dilution 100 times, grind off pericarp with cleaning ball, then under flowing water, more than 2h is rinsed, with 75% alcohol sterilizing 1min in superclean bench, then use 4% liquor natrii hypochloritis's sterilizing 30min, with aseptic water washing 5-6 time;
(2) cut the fruit of sterilizing open, take out seed, cut away seed wing with sterile scalpel, be advisable to expose seed, be inoculated in the 1/2MS minimal medium containing 0.5g/L active carbon, light culture 1-2 week, seed starts to sprout seedling, cultivates under being then transferred to light;
Minimal medium is: 1/2MS+0.5g/L active carbon+30g/L sucrose+6.5g/L agar, pH5.8-6.0; Cultivation temperature 25 ± 2 DEG C, light application time 12-14h/d, intensity of illumination 2000-3000lx;
(3) select highly more than the aseptic seeding seedling of 2cm, cut off root and unnecessary blade by sterile scissors, be inoculated in inducing clumping bud medium, carry out inducing clumping bud;
Inducing clumping bud medium is: WPM+0.5-1.0mg/L6-BA+0.025-0.1mg/LIBA+20g/L sucrose+6.5g/L agar, pH5.2-5.4; Cultivation temperature 25 ± 2 DEG C, light application time 12-14h/d, intensity of illumination 2000-3000lx;
(4), after treating that inducing clumping bud cultivates 5-6 week, select highly more than 3cm, and the Multiple Buds of robust growth is inoculated in root induction medium, carries out root induction;
Root induction medium is: 1/2MS+0.025-0.05mg/L6-BA+0.1-1.0mg/LIBA+0.5g/L active carbon+30g/L sucrose+6.5g/L agar, pH5.8-6.0; Cultivation temperature 25 ± 2 DEG C, light application time 12-14h/d, intensity of illumination 2000-3000lx;
(5) 3-4cm length is reached when aseptic seedling root growth is intact, and when being no less than 5, open sealed membrane and train indoor hardening 1 week in group, then the medium of plantlet in vitro root is washed away with sterile water, transplanting to the sterilized peat composed of rotten mosses and perlite volume ratio is in the mixed-matrix of 3:1, and bagging keeps humidity, and keeps spray water every day 2-3 time, then reduce humidity gradually and increase intensity of illumination, after 2 weeks, after transplanted seedling survives, in greenhouse, carrying out cellar culture.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410394862.7A CN104186317B (en) | 2014-08-12 | 2014-08-12 | The tissue culture and rapid propagation method of Yunnan crape myrtle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410394862.7A CN104186317B (en) | 2014-08-12 | 2014-08-12 | The tissue culture and rapid propagation method of Yunnan crape myrtle |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104186317A CN104186317A (en) | 2014-12-10 |
| CN104186317B true CN104186317B (en) | 2016-04-27 |
Family
ID=52071838
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410394862.7A Active CN104186317B (en) | 2014-08-12 | 2014-08-12 | The tissue culture and rapid propagation method of Yunnan crape myrtle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104186317B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104604735B (en) * | 2015-03-11 | 2017-03-15 | 华南农业大学 | A kind of tissue culture and rapid propagation method of U.S.'s red autumnal leaves Lagerstroemia indica L. |
| CN105230481B (en) * | 2015-04-27 | 2017-05-10 | 闽南师范大学 | Spathodea campanulata callus induction method |
| CN109392679A (en) * | 2018-10-30 | 2019-03-01 | 浙江农林大学 | A kind of aseptic seeding propagation method of Huang common vetch |
| CN109169289A (en) * | 2018-11-06 | 2019-01-11 | 湖南省林业科学院 | A kind of crape myrtle tissue culture proliferation seedling rooting medicament and method |
| CN110313404A (en) * | 2019-08-13 | 2019-10-11 | 湖南省林业科学院 | A kind of construction method of crape myrtle Tissue Culture Regeneration System |
| CN113557954B (en) * | 2021-07-06 | 2022-06-24 | 北京林业大学 | Method for obtaining hybrid between lagerstroemia and lagerstroemia |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100693400B1 (en) * | 2005-06-30 | 2007-03-09 | 주식회사 삼양제넥스 | The method for production of corosolic acid in suspension culture of plant cells |
| CN101707982B (en) * | 2009-11-18 | 2011-09-07 | 江苏省中国科学院植物研究所 | Culture and reproduction method of Lagerstroemia fauriei Keohne tissues |
| CN102726190A (en) * | 2012-06-29 | 2012-10-17 | 句容市仑山花木场 | Cuttage seedling culture method for red lagerstroemia speciosa |
-
2014
- 2014-08-12 CN CN201410394862.7A patent/CN104186317B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN104186317A (en) | 2014-12-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104186317B (en) | The tissue culture and rapid propagation method of Yunnan crape myrtle | |
| CN101366357B (en) | Method for tissue culture and quick propagation technique of reddish blue spider lily | |
| CN102648698B (en) | Pyrus stem tip tissue culture rapid propagation method | |
| CN104041412B (en) | The quick breeding method for tissue culture of a kind of Guizhou half capsule lettuce tongue | |
| CN105475130A (en) | Castanopsis hystrix high efficiency isolated culture plant regeneration method | |
| CN104012417B (en) | High-efficiency and rapid micropropagation method for toxicodendron vernicifluum | |
| CN105104202B (en) | A kind of preserving seed method of African Chrysanthemum tissue culture propagation | |
| CN104855292B (en) | A kind of method of Cinnamomum kanahirai hay stem segment tissue culture fast breeding | |
| CN104871679B (en) | A kind of Cyclobanopsis chungii seed equilibrium breaking dormancy and sprout cultivating method | |
| CN103931497A (en) | Method for improving seedling rate of tissue culture seedlings of hylocereus undulatus britt | |
| CN108739370B (en) | Method for rapid propagation by utilizing mature lotus embryos | |
| CN105766654B (en) | A kind of Nanchuan jackfruit method for tissue culture | |
| CN107278891B (en) | A kind of apricot plum quick breeding method for tissue culture | |
| CN104145814B (en) | Method for obtaining regeneration plants by stem tissue culture of cerasus cerasoides (var. cerasoides) | |
| CN104145818A (en) | Preservation method of gerbera germplasm resources | |
| CN102210267A (en) | Method for regenerating rose into complete plant | |
| CN105532459B (en) | A kind of tissue culture and rapid propagation method of Acer palmatum orange dream | |
| CN104756866A (en) | Cuttage rapid propagation method of test-tube plantlet of toona sinensis | |
| CN105123526B (en) | Germplasm storage method of zinnia elegan tissue culture propagation | |
| CN105941155B (en) | A kind of method that Manchurian ash is quickly bred using suspension culture techniques | |
| CN108651275A (en) | A kind of method of quickly breeding bletilla striata seedling under natural light | |
| CN101564010B (en) | Method for rapidly propagating tupelos | |
| CN107466852B (en) | A kind of thick stem Japanese ardisia tissue cultures and rapid propagation method | |
| CN106386499A (en) | Tissue cultured rapid propagation method for Rhizoma Stahlianthi Involucrati | |
| CN105660397A (en) | High-frequency regeneration and rapid propagation method of Enkianthus chinensis tissue culture seedlings |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |