CN105211106A - A kind of granule containing trichoderma harzianum and humus - Google Patents
A kind of granule containing trichoderma harzianum and humus Download PDFInfo
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- CN105211106A CN105211106A CN201510697434.6A CN201510697434A CN105211106A CN 105211106 A CN105211106 A CN 105211106A CN 201510697434 A CN201510697434 A CN 201510697434A CN 105211106 A CN105211106 A CN 105211106A
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Abstract
The present invention relates to a kind of granule containing trichoderma harzianum and humus, containing active component trichoderma harzianum, hymexazo and humus.In described granule, be set as 100% female medicine with the Trichoderma harzianum starter medicine that content is 30,000,000,000 CFU/ grams, the weight ratio of hymexazo, trichoderma harzianum and humus is 1-25:1-5:10-80.The present invention is prepared into granule, and medicament is easy to use, by spreading fertilizer over the fields, spreading manuer in holes or ditch spread, without the need to being watered spraying, after overcoming soil relatively dry, peasant just carries out being watered the delayed control of spray medicine, be conducive to germ after the rain occurrence in peak period can prevent and treat timely, reduce the loss.Active component has Synergistic function antibacterial, and rational ratio mixture can reduce the usage amount of each single dose, contributes to saving drug cost.
Description
Technical field
The present invention relates to cultural control technical field, specifically a kind of granule containing trichoderma harzianum and humus.
Background technology
Soil-borne disease refers to that pathogene such as fungi, bacterium, nematode and virus are lived in soil with invalid body, the disease caused from crop root or stem infringement crop when condition is suitable for.The continuous cropping in soil, the raising of cropping index, and the unreasonable of fertilising makes soil-borne disease increasing reason in recent years.Damping off, root rot, damping off are the Major Diseases of crops seedling stage, generally make minority death of seedling, cause and lack the disconnected ridge of row, even cause seedling to lodge in flakes dead serious consequence.
The pathogen of soil-borne disease is before crop-planting just Already in soil, current people also do not form the custom of medication prevention, generally after disease occurs, just make with medicament be watered spraying control, if the rainy weather morbidity running into soil moisture large is heavier, be watered spray cumbersome, and be watered spray do not meet peasant often in soil or crop leaf more dry when medication, disease can not get preventing and treating timely, causes the massive losses of part death of seedling.
In view of above problem, soil-borne disease medicament is prepared into granule, after crop sowing time, transplanting time or rain, soil moisture is used greatly, and has good control efficiency, is the main path solved the problems of the technologies described above.
Trichoderma harzianum is the microbial bactericide produced by Bai Wo limited company of the U.S., is mainly used in the fungal disease preventing and treating plant root.Trichoderma harzianum effectively can not only suppress the growth of germ, and can produce the compound of stimulating plant growth and inducing plant defense reaction, improves the microenvironment of root system, strengthens growing way and the resistance against diseases of plant, improves output and the income of crop.
Hymexazo, chemical name: 3-hydroxy-5-methyl base isoxazole is the conventional medicament preventing and treating soil-borne disease, has good prevention effect to by pythium spp, rhizoctonia, the microbial damping off of reaping hook, damping off, root rot.Hymexazo has interior absorption, by the root absorption of plant and can move in root system, produce two kinds of glucosides, crop is improved to the effect of physiologically active, thus can promote plant strain growth at plant intracellular metabolite, the tillering of root, the increase of root hair and the raising of root activity.
Humic acid is a kind of natural organic high molecular compound, and complex structure, has the functions such as ion exchange, chelating, flocculation and colloidal sol, and improvement soil, stimulating growth, enhancing are degeneration-resistant, the effect of oil recovery enhancement agriculturally having.Humus and combined use of pesticides have synergistic effect, can reduce the usage amount of agricultural chemicals, extend the duration of drug effect.The control that humus is used for soil-borne disease has relevant report, " humic acid biological and ecological methods to prevent plant disease, pests, and erosion biological organic fertilizer is to the research of Fusarium solani control efficiency " delivered as highlighted is pointed out, fertilizer containing humic acid and biocontrol microorganisms effectively can reduce the occurrence and harm of Fusarium solani, improves control efficiency.
In agricultural production, carry out disease control with single medicament for a long time, germ easily produces resistance, and control efficiency is undesirable.By the mutual mixture between agricultural chemicals, be improve drug effect, extend the convenient of medicament life cycle and effective approach.
Current ， is not also shown in relevant report using hymexazo and trichoderma harzianum and humus as active ingredient particle mixed preparation.
Summary of the invention
The object of the invention is to, provide a kind of soil-borne disease period of disease that is applicable to carry out medication control, and meet farming operating habit, and be conducive to delaying the drug-fast granule containing trichoderma harzianum and humus of germ.
The technical solution adopted in the present invention is: a kind of granule containing trichoderma harzianum and humus, containing active component trichoderma harzianum, hymexazo and humus.
In described granule, be set as 100% female medicine with the Trichoderma harzianum starter medicine that content is 30,000,000,000 CFU/ grams, the weight ratio of hymexazo, trichoderma harzianum and humus is 1-25:1-5:10-80, is preferably 1-5:1-3:30-50
The described percentage by weight of granule Zhong ， hymexazo in granule is 0.1%-2.5%, and the percentage by weight of trichoderma harzianum in granule is 0.1%-1.0%, and humus percentage by weight in granule is 2-8%.
The described adjuvant used containing the granule of trichoderma harzianum and humus comprise filler, binding agent, dispersant, wetting agent, disintegrant, sustained release agent, stabilizing agent or other be of value to the active ingredient material that plays of stable and drug effect in the formulation, all various compositions that are conventional in pesticidal preparations or permission use, be not particularly limited, concrete composition and consumption are determined by simple experiment according to recipe requirements.
The present invention has the following advantages and significant technique effect:
(1) be prepared into granule, in conjunction with farming operating habit, be conducive to soil moisture and become a fine day the feature that the acceptant granule of peasant carries out dispenser greatly and after rain.
(2) medicament is easy to use, and by spreading fertilizer over the fields, spreading manuer in holes or ditch spread, without the need to being watered spraying, after overcoming soil relatively dry, peasant just carries out being watered the delayed control of spray medicine, be conducive to germ after the rain occurrence in peak period can prevent and treat timely, reduce the loss.
(3) property of living becomes point hymexazo, trichoderma harzianum and a humus to have Synergistic function antibacterial, and rational ratio mixture can reduce the usage amount of each single dose, contributes to saving drug cost.
Embodiment
Be described further technical scheme of the present invention and technique effect below in conjunction with specific embodiment, percentage of the present invention is all weight percentage.
The embodiment of the present invention is the method adopting Toxicity Determination and field trial to combine.
Embodiment 1: hymexazo, trichoderma harzianum, humus and mixture preparation are to the Toxicity Determination of rice seedling blight
Subjects: rice seedling blight (Rhizoctoniasolani), is gathered by field, through indoor separation and purification.
Test method: determine that after the effective inhibition concentration scope of each medicament, hymexazo, trichoderma harzianum, humus and mixture preparation thereof are diluted 5 concentration by active constituent content gradient series by ，, compare with sterile water through prerun.Carry out with reference to " People's Republic of China (PRC) agricultural industry criteria NY/T1156.2-2006 ", adopt mycelial growth rate method to measure.Variable concentrations medicament is added in sterilizing culture dish, every ware 1mL, add the PDA medium 10mL of sterilizing fusing again, fully be mixed and made into flat board, again the bacterium colony spread cultivation is broken into=bacterium the dish of 0.8cm, 1, every ware, often process 4 times and repeat, be placed in 25 DEG C of insulating boxs to cultivate, every 24h measures colony diameter 1 time.Calculate mycelial growth inhibition rate, obtain toxicity regression formula, calculate EC
50, mycelial growth inhibition rate formula is as follows:
Mycelial growth inhibition rate (%)=(blank bacterium colony increases diameter-chemicals treatment bacterium colony and increases diameter)/blank bacterium colony increases diameter × 100
Evaluate the synergistic effect of pharmacy mix with the co-toxicity coefficient method (CTC) of the abundant method of Sun Yun, namely CTC≤80 are antagonism, and 80<CTC<120 is summation action, and CTC >=120 are synergistic effect.
Toxicity test the results are shown in Table 1.
Table 1 hymexazo, trichoderma harzianum, humus and mixture preparation are to the Toxicity Determination table of rice seedling blight
， hymexazo, trichoderma harzianum and humus are to the EC of rice wilt pathogens as shown in Table 1
50be respectively 22.75mg/L, 3.51mg/L and 106.39mg/L ， when the weight ratio of hymexazo, trichoderma harzianum and humus is at 1-25:1-5:10-80, CTC is all greater than 120, has good synergistic function
Wherein the weight ratio of Dang hymexazo, trichoderma harzianum and humus is 1:1:50, co-toxicity coefficient (CTC) is 268.7, show good synergistic relationship when the weight ratio of hymexazo and trichoderma harzianum is 1:8:100, co-toxicity coefficient (CTC) is that 73.6 ， hymexazos, trichoderma harzianum and humus mixture produce antagonism.
Embodiment 2:0.5% hymexazo 0.3 hundred million cfu/ gram of trichoderma harzianum 5% humus granule
When the weight ratio of Yi Ju hymexazo, trichoderma harzianum and humus is 5:1:50, composition has synergistic function, take according to quantity, the former medicine 0.53kg of 95% hymexazo, 30,000,000,000 cfu/ grams of Trichoderma harzianum starter medicine 0.1kg, humus 5kg, naphthalenesulfonate formaldehyde condensation compound 1.5kg, lauryl sodium sulfate 2.5kg, polyvinyl alcohol 5.0kg, white carbon 15kg, bentonite complements to 100kg.
Above-mentioned recipe ingredient is mixed, pulverizes, stir after adding water-wet, then use screw extruder pelletizer granulation, sieve after drying and obtain 0.5% hymexazo 0.3 hundred million cfu/ gram of trichoderma harzianum 5% humus granule.
Formulation application embodiment 2 be prepared into is in the field experiment of control capsicum damping off
(1) experimental scheme
Preparation embodiment 2 be prepared into is as experimental group 1#, and amount of application is 0.25 kilogram/m
2; Respectively with 2.5% hymexazol granule 1# as a control group, 1.0 hundred million cfu/ grams of trichoderma harzianum granule 2# as a control group, 10% humus granule 3# as a control group, amount of application is respectively 0.5 kilogram/m
2, 1 blank group is set.
(2) application method
Select the plot that continuous cropping capsicum damping off is serious, nursery soil carries the germs of a disease.In the first time dispenser of capsicum tidy seedlings output sequela initial stage, interval 7d repeats dispenser once, evenly spreads fertilizer over the fields on seedbed, each process 3 repetition by the medicament of experimental scheme by experimental group 1#, control group 1#, control group 2#, control group 3#, each seedling bed as a community, plot area 0.5m
2, in each community, keep seedbed environment consistent.Investigate dead seedling number respectively at 10d after 7d, second time dispenser after first time dispenser, calculate death rate and preventive effect.
(3) result of the test
First time dispenser after 7 days, the preventive effect of experimental group 1# is 92.26%; The preventive effect of control group 1# is 78.53%; The preventive effect of control group 2# is 72.43%; The preventive effect 56.13% of control group 3#.Second time dispenser after 10 days, the preventive effect of experimental group 1# is 88.54%; The preventive effect of control group 1# is 74.35%; The preventive effect of control group 2# is 71.46%; The preventive effect 52.25% of control group 3#.Yin Ci ， hymexazo, trichoderma harzianum and humus mixture, obvious to the synergistic effect of capsicum damping off, both reduce the usage amount of active ingredient, show again the field efficacy being better than single dose.
Embodiment 3:0.1% hymexazo 0.3 hundred million cfu/ gram of trichoderma harzianum 3% humus granule
When the weight ratio of Yi Ju hymexazo, trichoderma harzianum and humus is 1:1:30, composition has synergistic effect, take according to quantity, 95% hymexazo 0.21kg, 30,000,000,000 cfu/ grams of Trichoderma harzianum starter medicine 0.2kg, humus 6.0kg, fatty alcohol-polyoxyethylene ether 5.0kg, lauryl sodium sulfate 7.5kg, carboxymethyl cellulose 2.5kg, kaolin 30kg, diatomite complements to 200kg.
Above-mentioned recipe ingredient is mixed, pulverizes, stir after adding water-wet, then use screw extruder pelletizer granulation, sieve after drying and obtain 0.1% hymexazo 0.3 hundred million cfu/ gram of trichoderma harzianum 3% humus granule.
Formulation application embodiment 3 be prepared into is in the field medicine effect test of control cucumber at seedling stage damping off
(1) experimental scheme
Preparation embodiment 3 be prepared into is as experimental group 1#, and amount of application is 20 kgs/acre; Respectively with 1.5% hymexazol granule 1# as a control group, 1.0 hundred million cfu/ grams of trichoderma harzianum granule 2# as a control group, 10% humus granule 3# as a control group, amount of application is respectively 45 kgs/acre, arranges 1 blank group.
(2) application method
Select continuous cropping cucumber rhizoctonia rot that serious plot occurs, by experimental scheme, the medicament of experimental group 1#, control group 1#, control group 2#, control group 3# and consumption are evenly mixed soil in sowing cave, the cucumber seeds into having urged bud is planted afterwards, 3, every cave cucumber seedling, each process 100 cave in sowing cave.Keep soil moisture consistent in each process, the operation of other farming is normally carried out.
(3) investigation method
After dispenser, add up incidence after 18 days, calculate control efficiency and the state of an illness is divided into 5 grades: 0 grade for plant and there is no Disease symptoms; 1 grade is the slight withered and yellow symptom of plant radical leaves appearance, and slight brown stain appears in root system; 2 grades there is withered and yellow symptom for plant middle and lower part, and brown stain appears in root system, but terminal bud is good; 3 grades there is withered and yellow symptom for complete stool, and root system brown stain is rotted; 4 grades is plant withered death, and the serious brown stain of root system is rotted.
Diseased plant rate (%)=diseased plant number/investigate total strain number × 100%
Disease index=∑ (morbidity number at different levels × this level represents number)/(sum × highest typical value) × 100
Control efficiency (%)=(contrast disease index-process disease index)/contrast disease index × 100%
(4) result of the test
Through statistical computation, the control efficiency of experimental group 1# is 85.36%; The control efficiency 65.47% of the control efficiency 62.53% control group 2# of control group 1#; Control group 3# control efficiency 57.38%.Can find out that from test the composite rear synergy of ， hymexazo, trichoderma harzianum and humus is obvious, both reduce the usage amount of active ingredient, and shown again the field efficacy being better than single dose.
Claims (3)
1. one kind contains the granule of trichoderma harzianum and humus, it is characterized in that, active component is made up of trichoderma harzianum, hymexazo and humus, in described active component, be set as 100% female medicine with the Trichoderma harzianum starter medicine that content is 30,000,000,000 CFU/ grams, the weight ratio of hymexazo, trichoderma harzianum and humus is 1-25:1-5:10-80.
2. the granule containing trichoderma harzianum and humus according to claim 1, is characterized in that the weight ratio of ， hymexazo, trichoderma harzianum and humus is 1-5:1-3:30-50.
3. the granule containing trichoderma harzianum and humus according to claim 1 and 2, it is characterized in that the percentage by weight of hymexazo in granule is 0.1%-2.5%, the percentage by weight of trichoderma harzianum in granule is 0.1%-1.0%, and humus percentage by weight in granule is 2-8%.
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| CN103588544A (en) * | 2012-08-13 | 2014-02-19 | 北京中农广袤农业科技有限公司 | Microbial organic fertilizer, and preparation method and purpose thereof |
| CN104151113A (en) * | 2014-08-29 | 2014-11-19 | 上海大井生物工程有限公司 | Formula of trichoderma harzianum microbial fertilizer for soil remediation and preparation process thereof |
| WO2015011615A1 (en) * | 2013-07-22 | 2015-01-29 | Basf Corporation | Mixtures comprising a trichoderma strain and a pesticide |
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2015
- 2015-10-26 CN CN201510697434.6A patent/CN105211106B/en active Active
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| CN101696390A (en) * | 2009-10-29 | 2010-04-21 | 南京农业大学 | Biological preventing and controlling strain of continuous cropping cucumber and watermelon blight and microbe organic fertilizer thereof |
| CN103588544A (en) * | 2012-08-13 | 2014-02-19 | 北京中农广袤农业科技有限公司 | Microbial organic fertilizer, and preparation method and purpose thereof |
| CN103250731A (en) * | 2013-05-30 | 2013-08-21 | 王鲁 | Insecticide sterilization compound agent containing hymexazol |
| WO2015011615A1 (en) * | 2013-07-22 | 2015-01-29 | Basf Corporation | Mixtures comprising a trichoderma strain and a pesticide |
| CN104151113A (en) * | 2014-08-29 | 2014-11-19 | 上海大井生物工程有限公司 | Formula of trichoderma harzianum microbial fertilizer for soil remediation and preparation process thereof |
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