CN105191985A - Combination containing bacillus subtilis, kasugamycin and tricyclazole and application - Google Patents
Combination containing bacillus subtilis, kasugamycin and tricyclazole and application Download PDFInfo
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- CN105191985A CN105191985A CN201510609737.8A CN201510609737A CN105191985A CN 105191985 A CN105191985 A CN 105191985A CN 201510609737 A CN201510609737 A CN 201510609737A CN 105191985 A CN105191985 A CN 105191985A
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- bacillus subtilis
- tricyclazole
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- kasugarnycin
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Abstract
The invention discloses a combination containing bacillus subtilis, kasugamycin and tricyclazole and an application of the combination in pesticides. It is found by studies that a mixture composited by a microbial agent (bacillus subtilis), agricultural antibiotics fungicide (kasugamycin) and chemical pesticides fungicide (tricyclazole) can not only improve the original efficacy for preventing and curing rice blast, rice sheath blight disease and the like but also can prevent and cure new disease, the current situation that the efficacy of the three are poor due to single agent form for a long time is changed, the use amount of chemical pesticide is effectively reduced, the residue of pesticides on rice is reduced, the crop quality is improved, and the food crop safety is guaranteed.
Description
Technical field
The present invention relates to biological pesticide technical field, be specifically related to a kind of composition containing bacillus subtilis, kasugarnycin and tricyclazole and application.
Background technology
China is Rice Production maximum in the world and country of consumption.Paddy rice Annual planting area about 2,860 ten thousand hectares, produces 1.85 hundred million tons, rice per year, accounts for 1/5 of global Monitoring of Paddy Rice Plant Area, account for 1/3 of View of World Rice gross yield.Paddy always produces every year and ranks first in the world, and average per unit area yield is 1.6 times of world average level.But along with Chinese national economy development and the raising of people's lives quality, more and more higher to the cry of grain security.In recent years, due to the impact of the factors such as weather, environment and planting patterns are unreasonable, rice fungus diseases evil is frequent to be occurred, and serious harm rice leaf, causes rice nutrition to lack, the general underproduction 10% ~ 20%, and severe one can reach more than 30%, sometimes even has no harvest.
For the control of rice fungus diseases evil, mainly at present to select from resistant rice cultivars, the aspect research such as the biological property of paddy rice germ, chemical control aspect is mainly still based on chemical agent.
Qiao Jinling etc. have studied the virulence of several bactericide to rice fungus diseases evil, compare the toxicity test of 13 kinds of bactericide to the common 5 kinds of fungies of paddy rice, comprise that alkene bacterium kills for examination bactericide, very spirit, detoxification, rice blast madly kill, rice seedlings peace, golden man of great strength, Isoprothiolane, prosperity, wither, sterilizing becomes, poison is clear, guarantor's standing grain is sharp and Fu meter Le.Be mainly chemical agent, wherein to the most obvious species medicine of fungi inhibitory action, conk inhibiting rate is (toxicity test that several bactericide does harm to rice fungus diseases, Qiao Jinling etc., agricultural modernization, the 2nd phase in 2012: 5 ~ 6) more than 80%.
The usage amount cumulative year after year of chemical bactericide, the chemical pesticide residue problem of cereal crops has a strong impact on food security, therefore, develops a kind of green, low toxicity, the biopesticide Shi Ge scientific research institution of environmental protection, the instant responsibility of enterprise.
In view of this, special proposition the present invention.
Summary of the invention
Undesirable in order to solve in prior art rice fungus diseases evil pesticide efficacy, and have the problems such as harmful substances is residual, the invention provides a kind of composition containing bacillus subtilis, kasugarnycin and tricyclazole.
Bacillus subtilis is the one of Bacillus, belongs to gram-positive bacteria.The subtilin that bacillus subtilis thalline produces in process of growth, polymixin, nystatin, gramicidins isoreactivity material have obvious inhibitory action to rice blast pathogenic bacteria, inducing plant self-defensive system resists the invasion of pathogen simultaneously, thus realizes the object of biological control.
Kasugarnycin, English name Kasugamycin, chemical name is (5-amino-2-methyl-6-(2,3,4,5,6-hydroxy-cyclohexyl oxo) tetrahydropyran-3-base) amino-α-IMDA, be a kind of farm antibiotics series bactericidal agent, have excellent preventive effect and therapeutic action to the rice blast on paddy rice.
Tricyclazole, English tricyclazole by name, chemical name is 5-methyl isophthalic acid, 2,4-triazol [3,4-B] benzothiazole, it is the special chemical bactericide of control rice blast, bactericidal action mechanism mainly suppresses the melanic formation of attachment spore, thus suppresses spore germination and attachment spore to be formed, and stops germ to invade people and reduces the generation of magnaporthe grisea spore.
Research finds, above-mentioned three kinds of compositions combine, and kasugarnycin and tricyclazole can not affect the biologic activity of bacillus subtilis, and the drug effect of three kinds of composition combinations is obviously better than being used alone or being combined between two.In addition, except rice blast, inventor finds that said composition has good control efficiency for rice sheath blight disease.
The present invention also provides the above-mentioned composition containing bacillus subtilis, kasugarnycin and tricyclazole preparing the application in agricultural chemicals.Preferably this agricultural chemicals is for preventing and treating paddy rice fungal disease.
The invention provides a kind of agricultural chemicals, composition comprises bacillus subtilis, kasugarnycin, tricyclazole, auxiliary agent and fills auxiliary material.
Preferably, in above-mentioned agricultural chemicals, the living bacteria count of described bacillus subtilis is 0.5 hundred million/gram ~ 1,000 hundred million/gram.
Preferably, above-mentioned agricultural chemicals is wetting powder, and the mass percentage of each composition is: bacillus subtilis 10% ~ 50%, kasugarnycin 4% ~ 13%, tricyclazole 4% ~ 50%, auxiliary agent 4% ~ 10%, fills auxiliary material surplus;
Described auxiliary agent is two or more arbitrarily in wetting agent, dispersant, stabilizing agent, synergist, bleeding agent, binding agent and disintegrant; Described filling auxiliary material is one or more in diatomite, kaolin, potter's clay, attapulgite.
Preferably, in above-mentioned agricultural chemicals, described dispersant is neopelex, sodium lignin sulfonate, sodium butylnaphthalenesulfonate, succinic acid alkyl ester sulfonate sodium or dispersing agent NNO, and described bleeding agent is bleeding agent JFO.
Preferably, in above-mentioned agricultural chemicals, the mass percentage of each composition is: bacillus subtilis 10% ~ 50%, kasugarnycin 4% ~ 13%, tricyclazole 4% ~ 50%, auxiliary agent 6% ~ 9%, diatomite surplus; Wherein auxiliary agent is dispersing agent NNO 3% and succinic acid alkyl ester sulfonate sodium 3 ~ 6%.
Preferably, this agricultural chemicals is for preventing and treating paddy rice fungal disease.Especially rice blast and/or rice sheath blight disease.
The present invention also provides the preparation method of above-mentioned agricultural chemicals, is bacillus subtilis to be inoculated into liquid ventilation cultivation in fermentation tank to obtain zymocyte liquid, bacteria concentration > 1 × 10
11individual/mL; Zymocyte liquid is through the centrifugal 10min ~ 15min of 5500rpm ~ 6600rpm rotating speed, and abandon supernatant collecting precipitation bacterium mud, bacterium mud spraying dry makes bacterium powder;
Bacterium powder, kasugarnycin, tricyclazole, auxiliary agent and filling auxiliary material Homogeneous phase mixing at ambient temperature in proportion, is prepared into wetting powder.
The present invention compared with prior art has following beneficial effect:
The present invention is by composite to microbial bacterial agent (bacillus subtilis), agricultural antibiotic fungicide (kasugarnycin) and chemical pesticide bactericide (tricyclazole), find this composite after mixture not only can improve the drug effect of original control rice blast, rice sheath blight disease etc., but also new disease can be prevented and treated, change three for a long time with the not good enough present situation of unit dose form drug effect, the usage amount of effective minimizing chemical pesticide, reduce the persticide residue on rice, improve crop quality, ensure that cereal crops safety.
Embodiment
Below in conjunction with specific embodiment, the invention will be further described, to help understanding content of the present invention.
One, example of formulations
Kasugarnycin is the former medicine of kasugarnycin (kasugarnycin content 55% ~ 70%, not diluted), tricyclazole is Mensurating Tricyclazole Technical (tricyclazole content more than 95%, not diluted), bacillus subtilis (Chinese agriculture Microbiological Culture Collection administrative center (ACCC), deposit number is ACCC10089).
A kind of wetting powder, is made up of the raw material of following percentage: bacillus subtilis 10% ~ 50%, kasugarnycin 4% ~ 13%, tricyclazole 4% ~ 50%, auxiliary agent 4% ~ 10%, fills auxiliary material 20% ~ 70%.
Wherein, described bacillus subtilis living bacteria count be 0.5 hundred million/gram ~ 1,000 hundred million/gram.
Auxiliary agent is two or more in wetting agent, dispersant, stabilizing agent, synergist, bleeding agent, binding agent, disintegrant; Filling auxiliary material is one or more in diatomite, kaolin, potter's clay, attapulgite.
Described bacillus subtilis is carried out deep liquid High Density Cultivation, obtains zymotic fluid, and ensure to be greater than 1 × 10 containing living bacteria count in every milliliter of zymotic fluid
11individual.
Carry out high speed centrifugation process by pre-configured centrifuge to above-mentioned zymotic fluid, collect bacterium mud, wherein, the rotating speed of described centrifuge is 5500 ~ 6600rpm, and the time of described centrifugal treating is 10 ~ 15min;
By the bacterium mud spraying dry collected, make bacillus subtilis bacterium powder, for subsequent use;
By described bacillus subtilis, kasugarnycin, tricyclazole, auxiliary agent, filler Homogeneous phase mixing in mixed at room temperature machine in proportion, the wetting powder containing bacillus subtilis, kasugarnycin and tricyclazole can be obtained.
Conveniently understand technique scheme of the present invention, below by way of instantiation, technique scheme of the present invention is described in detail.Following examples Chinese medicine composition is weight portion, amounts to 100 weight portions, bacillus subtilis living bacteria count 1,000 hundred million/gram.
In table, NOO represents diffusion agent N OO, and A represents sodium butylnaphthalenesulfonate, and B represents succinic acid alkyl ester sulfonate sodium.
Two, preventive effect embodiment
Preventive effect embodiment one:
By the embodiment 1 ~ 3 of above-mentioned preparation and the wetting powder thin up of comparative example 1 ~ 3, spraying, be applied to control rice blast, bacillus subtilis, kasugarnycin, tricyclazole be mixed with respectively the wetting powder in contrast medicament identical with material active constituent content each in composition.Medicament fountain height is 100 grams/acre.The results are shown in preventive effect table one.
Preventive effect table
| Medicament kind | Active constituent content | Consumption | Control efficiency |
| Embodiment 1 | 32% | 100 grams/acre | 88.7% |
| Bacillus subtilis | 32% | 100 grams/acre | 72.3% |
| Kasugarnycin | 32% | 100 grams/acre | 60.8% |
| Tricyclazole | 32% | 100 grams/acre | 56.9% |
| Comparative example 1 | 32% | 100 grams/acre | 76.2% |
| Embodiment 2 | 40% | 75 grams/acre | 90.5% |
| Bacillus subtilis | 40% | 75 grams/acre | 75.5% |
| Kasugarnycin | 40% | 75 grams/acre | 45.2% |
| Tricyclazole | 40% | 75 grams/acre | 40.1% |
| Comparative example 2 | 40% | 75 grams/acre | 83% |
| Embodiment 3 | 63% | 50 grams/acre | 92.3% |
| Bacillus subtilis | 63% | 50 grams/acre | 75.6% |
| Kasugarnycin | 63% | 50 grams/acre | 58.7% |
| Tricyclazole | 63% | 50 grams/acre | 49.2% |
| Comparative example 3 | 63% | 50 grams/acre | 88.4% |
Preventive effect embodiment two:
By the embodiment 1 ~ 3 of above-mentioned preparation and the wetting powder thin up of comparative example 1 ~ 3, spraying, be applied to the water prevention rice sheath blight, bacillus subtilis, kasugarnycin, tricyclazole be mixed with respectively the wetting powder in contrast medicament identical with material active constituent content each in composition.Medicament fountain height is 100 grams/acre.The results are shown in preventive effect table two.
Preventive effect table two
| Medicament kind | Active constituent content | Consumption | Control efficiency |
| Embodiment 1 | 32% | 100 grams/acre | 86.3% |
| Bacillus subtilis | 32% | 100 grams/acre | 68.3% |
| Kasugarnycin | 32% | 100 grams/acre | 61.2% |
| Tricyclazole | 32% | 100 grams/acre | 54.3% |
| Comparative example 1 | 32% | 100 grams/acre | 75.6% |
| Embodiment 2 | 40% | 75 grams/acre | 87.9% |
| Bacillus subtilis | 40% | 75 grams/acre | 78.2% |
| Kasugarnycin | 40% | 75 grams/acre | 50.6% |
| Tricyclazole | 40% | 75 grams/acre | 46.8% |
| Comparative example 2 | 40% | 75 grams/acre | 80.6% |
| Embodiment 3 | 63% | 50 grams/acre | 91.9% |
| Bacillus subtilis | 63% | 50 grams/acre | 74.2% |
| Kasugarnycin | 63% | 50 grams/acre | 68.5% |
| Tricyclazole | 63% | 50 grams/acre | 51.6% |
| Comparative example 3 | 63% | 50 grams/acre | 86.4% |
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (10)
1. the composition containing bacillus subtilis, kasugarnycin and tricyclazole.
2. the composition containing bacillus subtilis, kasugarnycin and tricyclazole according to claim 1 is preparing the application in agricultural chemicals.
3. application according to claim 2, is characterized in that, agricultural chemicals is for preventing and treating paddy rice fungal disease.
4. an agricultural chemicals, composition comprises bacillus subtilis, kasugarnycin, tricyclazole, auxiliary agent and fills auxiliary material.
5. agricultural chemicals according to claim 4, is characterized in that, the living bacteria count of described bacillus subtilis is 0.5 hundred million/gram ~ 1,000 hundred million/gram.
6. agricultural chemicals according to claim 4, is characterized in that, is wetting powder, and the mass percentage of each composition is: bacillus subtilis 10% ~ 50%, kasugarnycin 4% ~ 13%, tricyclazole 4% ~ 50%, auxiliary agent 4% ~ 10%, fills auxiliary material surplus;
Described auxiliary agent is two or more arbitrarily in wetting agent, dispersant, stabilizing agent, synergist, bleeding agent, binding agent and disintegrant; Described filling auxiliary material is one or more in diatomite, kaolin, potter's clay, attapulgite.
7. agricultural chemicals according to claim 6, is characterized in that, described dispersant is neopelex, sodium lignin sulfonate, sodium butylnaphthalenesulfonate, succinic acid alkyl ester sulfonate sodium or dispersing agent NNO, and described bleeding agent is bleeding agent JFO.
8. agricultural chemicals according to claim 7, is characterized in that, the mass percentage of each composition is: bacillus subtilis 10% ~ 50%, kasugarnycin 4% ~ 13%, tricyclazole 4% ~ 50%, auxiliary agent 6% ~ 9%, diatomite surplus; Wherein auxiliary agent is dispersing agent NNO 3% and succinic acid alkyl ester sulfonate sodium 3 ~ 6%.
9. according to the agricultural chemicals described in claim 4 ~ 8, it is characterized in that, this agricultural chemicals is for preventing and treating paddy rice fungal disease.
10. the preparation method of the arbitrary described agricultural chemicals of claim 4 ~ 9, is characterized in that, bacillus subtilis is inoculated into liquid ventilation cultivation in fermentation tank and obtains zymocyte liquid, bacteria concentration > 1 × 10
11individual/mL; Zymocyte liquid is through the centrifugal 10min ~ 15min of 5500rpm ~ 6600rpm rotating speed, and abandon supernatant collecting precipitation bacterium mud, bacterium mud spraying dry makes bacterium powder;
Bacterium powder, kasugarnycin, tricyclazole, auxiliary agent and filling auxiliary material Homogeneous phase mixing at ambient temperature in proportion, is prepared into wetting powder.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510609737.8A CN105191985A (en) | 2015-09-23 | 2015-09-23 | Combination containing bacillus subtilis, kasugamycin and tricyclazole and application |
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| CN201510609737.8A CN105191985A (en) | 2015-09-23 | 2015-09-23 | Combination containing bacillus subtilis, kasugamycin and tricyclazole and application |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108651455A (en) * | 2018-04-23 | 2018-10-16 | 江苏邦盛生物科技有限责任公司 | A kind of Synergistic type tricyclazole suspending agent and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101697737A (en) * | 2009-11-09 | 2010-04-28 | 江苏省农业科学院 | Bacillus subtilis and compound wettable sterilizing powder and use thereof |
| CN102057921A (en) * | 2010-12-14 | 2011-05-18 | 江苏省绿盾植保农药实验有限公司 | Fungicide containing tricyclazole and kasugamycin |
| CN104542713A (en) * | 2014-12-22 | 2015-04-29 | 江苏省农业科学院 | Bacillus subtilis composition and application thereof |
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2015
- 2015-09-23 CN CN201510609737.8A patent/CN105191985A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101697737A (en) * | 2009-11-09 | 2010-04-28 | 江苏省农业科学院 | Bacillus subtilis and compound wettable sterilizing powder and use thereof |
| CN102057921A (en) * | 2010-12-14 | 2011-05-18 | 江苏省绿盾植保农药实验有限公司 | Fungicide containing tricyclazole and kasugamycin |
| CN104542713A (en) * | 2014-12-22 | 2015-04-29 | 江苏省农业科学院 | Bacillus subtilis composition and application thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108651455A (en) * | 2018-04-23 | 2018-10-16 | 江苏邦盛生物科技有限责任公司 | A kind of Synergistic type tricyclazole suspending agent and preparation method thereof |
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Application publication date: 20151230 |