CN103651578A - Composition containing kasugamycin and application - Google Patents

Composition containing kasugamycin and application Download PDF

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Publication number
CN103651578A
CN103651578A CN201310697647.XA CN201310697647A CN103651578A CN 103651578 A CN103651578 A CN 103651578A CN 201310697647 A CN201310697647 A CN 201310697647A CN 103651578 A CN103651578 A CN 103651578A
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bacillus subtilis
kasugarnycin
bactericidal composition
content
kasugamycin
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CN201310697647.XA
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Chinese (zh)
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李视辉
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Abstract

The invention relates to sterilizing agent composition with a synergistic effect. The sterilizing agent composition comprises effective active ingredients of kasugamycin and bacillus subtilis, wherein the content of kasugamycin accounts for 0.1%-20%, and the content of bacillus subtilis is 1.0-1,000.0 hundred million per gram. An auxiliary and an excipient are added into the effective active ingredients, the mixture can be prepared into wettable powder, an aqueous solution and granules, and the composition is mainly used for preventing and curing blight, powdery mildew, banded sclerotial blight, rice blast and the like.

Description

A kind of composition and application containing kasugarnycin
Technical field
The invention belongs to the technical field of agricultural chemicals, be specifically related to a kind ofly contain kasugarnycin and bacillus subtilis carries out composite composition, and the application aspect control corps diseases.
Background technology
Kasugarnycin (kasugamycin) chemical name:; (5-amino-2-methyl-6-(2; 3; 4; 5,6-hydroxy-cyclohexyl oxo) tetrahydropyran-3-base) amino-α-IMDA, is antibiotics microbial pesticide; mechanism is to disturb the esterase system of metabolism of amino acid; thereby affect the synthetic of protein, suppress mycelia and extend and cause cell granulations, but on spore germination without impact.Have protection, treatment and stronger systemic activity, its result for the treatment of is more remarkable, is the various bacteria of crop and the desirable medicaments of fungal disease such as control vegetables, melon and fruit and paddy rice.Permeability is strong and can in plant corpus, move, instant effect after spray medicine, and resistance of rainwater washing against, the lasting period is long, and can make melon leaf look dark green after dispenser and extend harvest time.
Bacillus subtilis (Bacillus subtilis), belong to Gram-positive bacillus, bacillus subtilis can be by successfully growing to plant rhizosphere, body surface or body surely, with the nutrition around of pathogen competitive plant, secretion antibacterial material is to suppress pathogen growth, inducing plant system of defense is resisted pathogen invasion simultaneously, thereby reaches the object of biological and ecological methods to prevent plant disease, pests, and erosion.
No matter be kasugarnycin, bacillus subtilis or other microbial bactericides, mostly is for a long time to occur with single dose form, in application, be subject to resistance, the lower effect of the factor impacts such as disease and cognition degree etc., all not as good as chemical pesticide preparation, are not given full play to the advantage of biopesticide.
Summary of the invention
The object of the invention is for bacillus subtilis complex preparation less, kasugarnycin easily produces poisoning and the germ problem such as develop immunity to drugs, and the invention provides a kind of microbicide compositions that kasugarnycin and bacillus subtilis be active ingredient of take.
The present invention also provides the application of described biological bactericide composition.
Biological bactericide composition of the present invention is mainly used in preventing and treating watermelon blight and rice blast.
Biological bactericide composition of the present invention proves through field trial, and its control efficiency is good, and composite by each active component, can further improve preventive effect simultaneously, and control efficiency is significantly better than single dose.
The composition that contains kasugarnycin, it is characterized in that said composition comprises kasugarnycin and bacillus subtilis, wherein kasugarnycin content is 0.1~20%, bacillus subtilis bacterial content is 1.0~1000.0 hundred million/gram, biological bactericide composition is that conventional method is processed into wetting powder or aqua.
Beneficial effect of the present invention is: kasugarnycin and two kinds of bactericide of bacillus subtilis are carried out composite; because the mechanism of action between the two is different; make complex preparation have protection, interior suction conduction function concurrently; expanded fungicidal spectrum; thereby applied widely; composite composition has synergistic effect in certain proportion, and not only control efficiency is good, has reduced dosage and drug cost.
Embodiment
Below in conjunction with embodiment, the invention will be further described, but the present invention is not limited thereto, and the formulation preparation method in embodiment is conventional method.
Embodiment 1:10.5% kasugarnycin bacillus subtilis aqua, comprises following component by weight:
Figure BDA0000441035380000021
By whole mixing of materials, after stirring, material is joined to sand mill and carry out sand milling, make material reach 2~5 μ m fineness, make kasugarnycin bacillus subtilis aqua.
Embodiment 2: kasugarnycin bacillus subtilis wetting powder, comprises following component by weight:
Figure BDA0000441035380000031
Said components is carried out to air-flow crushing, make kasugarnycin bacillus subtilis wetting powder.
Using method is: using method foliar spray, and 40 liters of amount of liquid medicine for mu, dispenser twice, leaf pest initial phase, Flag Leaves in Rice is extracted out first 7 days; Flag Leaves in Rice is extracted the breach phase out.
Test material
Reagent agent kasugarnycin bacillus subtilis WP
40% Isoprothiolane EC Zhejiang Weir reaches chemical industry
The 4% generous Chemical Co., Ltd. in kasugarnycin WP Hunan
40% tricyclazole SCHan agriculture Chemical Co., Ltd.
28% carbendazim SC Hebei, well ridge Guo Feng agricultural chemicals Co., Ltd
Field control effectiveness test was cut village in Yuyang town, Jixian, tianjin Municipality machete and is carried out experimental field rice cultivation throughout the year testing on August 15th, 2012, and Comparison of Management is meticulous, and rice blast is regular incidence evil.
Test method
Test is established 6 processing, 1: kasugarnycin bacillus subtilis WP(the present invention) 6 grams/667 ㎡, 2:40% Isoprothiolane EC50ml/667 ㎡, 3:4% kasugarnycin WP2.5 gram/667 ㎡, 4:40% tricyclazole SC24ml/667 ㎡, 5:28% well ridge carbendazim SC35ml/667 ㎡, 6: clear water contrast.3 repetitions are established in every processing, and every community area is 30 ㎡, community randomized arrangement, and the cultivation condition of all treatment regions is consistent.Leaf pest initial phase, Flag Leaves in Rice is extracted dispenser for the first time in first 7 days out; Flag Leaves in Rice is extracted the dispenser for the second time of breach phase out.Select the bodyguard of Shandong Wish Plant Protection Machinery Co., Ltd. knapsack hand sprayer even spraying.
Investigation method
Investigation method Wei Mei gets community 5 points, and every fixed 1 cave, carries out mark, prevents and treats leaf pest, and the front investigation morbidity of dispenser radix, investigates preventive effect for 10 days before secondary dispenser with after secondary dispenser; 10 days and field survey product investigation panicle blast disease index after secondary dispenser.
Drug effect computational methods
Figure BDA0000441035380000041
Figure BDA0000441035380000042
Figure BDA0000441035380000043
Figure BDA0000441035380000044
Result and discussion
Spraying time is August 15 for the first time, and spraying time is August 30 for the second time.Investigation disease index before dispenser, for the second time 10 days investigation preventive effects after dispenser.Result is as follows:
Effect table before and after table 1 control
Reagent agent Disease index before medicine Disease index after medicine Control efficiency (%)
Kasugarnycin bacillus subtilis 11.43 10.54 88.62
1 product 11.30 12.36 75.26
2 products 11.26 13.51 68.10
3 products 11.33 22.69 61.84
4 products 11.38 16.46 72.30
Clear water contrast 11.01 63.98 ——
As can be seen from Table 1, the mixture preparation of kasugarnycin and bacillus subtilis can effectively be prevented and treated rice blast, and is obviously better than the control efficiency of single dose.
By field control effectiveness test result, show; The composite preventive effect that improves of biopesticide, environmentally friendly, the tool space that grows a lot.

Claims (6)

1. containing effective active component, be a composition for kasugarnycin and bacillus subtilis, kasugarnycin content is 0.1~20%, bacillus subtilis bacterial content is 1.0~1000.0 hundred million/gram.
2. bactericidal composition according to claim 1, is characterized in that, described kasugarnycin and the weight ratio of bacillus subtilis be, kasugarnycin: bacillus subtilis=1:20~5:1.
3. bactericidal composition according to claim 1 and 2, is characterized in that, the weight percentage of described active ingredient is 1%~90%.
4. bactericidal composition according to claim 1, is characterized in that, said composition formulation comprises wetting powder, aqua.
5. bactericidal composition as claimed in claim 1, for preventing and treating the fungal disease on crop.
6. bactericidal composition as claimed in claim 1, for preventing and treating vegetables fusarium wilt, powdery mildew, banded sclerotial blight, rice blast.
CN201310697647.XA 2013-12-18 2013-12-18 Composition containing kasugamycin and application Pending CN103651578A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104542713A (en) * 2014-12-22 2015-04-29 江苏省农业科学院 Bacillus subtilis composition and application thereof
CN104798834A (en) * 2015-04-21 2015-07-29 苏州市新泾村农业基地专业合作社 Biopesticide suitable for cucumber downy mildew and preparation method thereof
CN105284896A (en) * 2015-10-10 2016-02-03 平顶山市玉龙山生态农业科技发展有限公司 Bactericide for preventing and treating watermelon fusarium wilt diseases and preparation method of bactericide
CN109258692A (en) * 2017-07-18 2019-01-25 郭少波 A kind of compounded combination pharmacy agent containing kasugarnycin and Pseudomonas fluorescence
CN113367138A (en) * 2021-07-17 2021-09-10 湖南省水稻研究所 Ecological pesticide for organic rice planting

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104542713A (en) * 2014-12-22 2015-04-29 江苏省农业科学院 Bacillus subtilis composition and application thereof
CN104798834A (en) * 2015-04-21 2015-07-29 苏州市新泾村农业基地专业合作社 Biopesticide suitable for cucumber downy mildew and preparation method thereof
CN105284896A (en) * 2015-10-10 2016-02-03 平顶山市玉龙山生态农业科技发展有限公司 Bactericide for preventing and treating watermelon fusarium wilt diseases and preparation method of bactericide
CN109258692A (en) * 2017-07-18 2019-01-25 郭少波 A kind of compounded combination pharmacy agent containing kasugarnycin and Pseudomonas fluorescence
CN113367138A (en) * 2021-07-17 2021-09-10 湖南省水稻研究所 Ecological pesticide for organic rice planting

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Application publication date: 20140326