CN104798834A

CN104798834A - Biopesticide suitable for cucumber downy mildew and preparation method thereof

Info

Publication number: CN104798834A
Application number: CN201510188279.5A
Authority: CN
Inventors: 浦建洪; 邱奇元
Original assignee: SUZHOU CITY XINJING VILLAGE AGRICULTURAL BASE SPECIALTY COOPERATIVES
Current assignee: SUZHOU CITY XINJING VILLAGE AGRICULTURAL BASE SPECIALTY COOPERATIVES
Priority date: 2015-04-21
Filing date: 2015-04-21
Publication date: 2015-07-29

Abstract

The invention discloses a biopesticide suitable for cucumber downy mildew and a preparation method thereof. By aiming at the characteristic of cucumber downy mildew and the mechanism of action of various biological agents and herbal medicine extracts, biological agents like maltophilia, catechinic acid, bacillus subtilis, kasugamycin, a licorice extract, a xanthium sibiricum extract and a solanum nigrum extract which have different efficacies and characteristics are compounded through reasonable screening. The invention further discloses an effective preparation method, the prepared biopesticide not only has a good action effect on cucumber downy mildew, but also can effectively avoid the reduction of output of cucumbers, is non-toxic, harmless, ecological and environment-friendly, can greatly reduce the dosage, pesticide application times and the cost of the biopesticide, and besides, the biopesticide is convenient in preparation, long in storage time and has a strong practical value.

Description

A kind of biopesticide being applicable to cucumber downy mildew and preparation method thereof

Technical field

The present invention relates to the planting technology of cucumber, especially relate to a kind of biopesticide being applicable to cucumber downy mildew and preparation method thereof.

Background technology

Cucumber downy mildew is one of important disease in green cucumber process, occur in country of more than 70, the world, be a kind of destructive disease, due to Pseudoperonospora cubensis short incubation period, spread speed is fast and destructive strong to host, its control is more difficult, since the nineties in 20th century, Vegetable produce scale expanding day, its occurrence and harm is in the trend expanded year by year, cause the significantly underproduction of cucumber yield, serious threat is taken in the crops of peasant.

Current chemical agent is still one of the most effective measures of control cucumber downy mildew, but, along with a large amount of Long-Time Service of systemic fungicide, some bactericide kinds create pesticide resistance in succession, and control number of times frequently causes, and residue of pesticide exceed standard, contaminated environment, the adverse influences such as harm humans is healthy, need exploitation on producing at present badly nontoxic, efficient novel biopesticide is to carry out the control of cucumber downy mildew.

Biopesticide refers to and utilizes biological living (fungi, bacterium, insect viruses, genetically modified organism, natural enemy etc.) or its metabolite (pheromones, growth hormone, Nafusaku, 2,4-D etc.) preparation killing or suppress is carried out for agricultural pest, also known as natural pesticide, mean and non-chemically synthesize, from natural chemical substance or life entity, and there is the effect of sterilization pesticide and insecticide pesticide.Biopesticide comprises the microorganisms such as the sick pathogenic nematode of worm, bacterium and virus, botanical derivative and insect pheromones etc.Important role is played the part of in the integration Pest Management system (IPM) that biopesticide uses in organic farming.

Summary of the invention

Goal of the invention: affect cucumber quality and output to solve in prior art existing cucumber downy mildew incidence of disease height, the problem of nothing biopesticide targetedly, the present invention proposes a kind of biopesticide being applicable to cucumber downy mildew and preparation method thereof.

Technical scheme: to achieve these objectives, the present invention takes following technical scheme: a kind of biopesticide being applicable to cucumber downy mildew, comprise the component of following percentage by weight: the bacillus subtilis wetting powder of the monad 200-300 times dilution of 5%-10%, the catechin 300-500 times of dilution of 10%-15%, 10%-25%, the kasugarnycin wetting powder of 5%-15%, the licorice of 3%-5%, the Siberian cocklebur extract of 3%-5%, the black nightshade extract of 3%-5%, the emulsifier of 1%-5%, the dispersant of 1%-5%, water surplus.

Further, described emulsifier is one or both in sucrose fatty acid ester and sucrose ester three polyglycereol.

Further, described dispersant is fatty acid dispersant.

Further, described licorice, Siberian cocklebur extract and black nightshade extract are water extract.

The above-mentioned preparation method being applicable to the biopesticide of cucumber downy mildew, comprises the following steps:

A () cultivates 48h at the monad 200-300 times dilution of aequum and catechin 300-500 times of dilution are mixed latter 35 DEG C-45 DEG C;

B () adds the water of the bacillus subtilis wetting powder of aequum, low kasugarnycin wetting powder, half amount in above-mentioned culture fluid, stir to cultivate 72h at latter 35 DEG C-45 DEG C and obtain culture fluid A;

C the water of the licorice of aequum, Siberian cocklebur extract, black nightshade extract, emulsifier, dispersant and remainder mixes and naturally cools to room temperature after 30-45min is boiled in heating and obtain culture fluid B by ();

D 40 DEG C-50 DEG C placement more than 48h after culture fluid A and culture fluid B mixing and stirring can be obtained required biopesticide finished product by ().

Further, in described step (b), agitating mode should at the uniform velocity stir in same direction.

Beneficial effect: a kind of biopesticide being applicable to cucumber downy mildew provided by the invention and preparation method thereof, for the characteristic of cucumber downy mildew and the mechanism of action of various bacteria agent and herb extracts, by monad, catechin, bacillus subtilis, low kasugarnycin, licorice, it is composite that Siberian cocklebur extract and this several bacteria agent with different efficacies and characteristic of black nightshade extract carry out reasonably screening, and disclose a kind of effective preparation method, the biopesticide made is not only good to the action effect of cucumber downy mildew, effectively avoid the generation of cucumber underproduction phenomenon, and it is nontoxic, ecological, environmental protective, significantly reduce dosage, spraying times and drug cost, and the preparation of this biopesticide is convenient, holding time is long, there is very strong practical value.

Embodiment

Embodiment 1:

A kind of biopesticide being applicable to cucumber downy mildew, comprise the component of following percentage by weight: the monad 200-300 times dilution of 5%, the catechin 300-500 times of dilution of 10%, the bacillus subtilis wetting powder of 10%, kasugarnycin wetting powder, the licorice of 3%, the Siberian cocklebur extract of 3% of 5%, the black nightshade extract of 3%, the sucrose fatty acid ester of 1%, the dispersant of 1%, water surplus.

Embodiment 2:

A kind of biopesticide being applicable to cucumber downy mildew, comprise the component of following percentage by weight: the monad 200-300 times dilution of 10%, the catechin 300-500 times of dilution of 15%, the bacillus subtilis wetting powder of 25%, kasugarnycin wetting powder, the licorice of 5%, the Siberian cocklebur extract of 5% of 15%, the black nightshade extract of 5%, sucrose ester three polyglycereol of 5%, the dispersant of 5%, water surplus.

Embodiment 3:

A kind of biopesticide being applicable to cucumber downy mildew, comprise the component of following percentage by weight: the monad 200-300 times dilution of 8%, the catechin 300-500 times of dilution of 13%, the bacillus subtilis wetting powder of 15%, kasugarnycin wetting powder, the licorice of 4%, the Siberian cocklebur extract of 4% of 10%, the black nightshade extract of 4%, the sucrose fatty acid ester of 3% and sucrose ester trimerization qualities of glycerin are than the mixture for 1:1, the dispersant of 3%, water surplus.

Embodiment 4

A () cultivates 48h at the monad 200-300 times dilution of 8% and the catechin 300-500 times of dilution of 13% are mixed latter 40 DEG C;

B () adds bacillus subtilis wetting powder, the low kasugarnycin wetting powder of 10%, the water of half amount of 15% in above-mentioned culture fluid, stir to cultivate 72h at latter 40 DEG C and obtain culture fluid A; Wherein agitating mode should at the uniform velocity stir in same direction;

(c) by the licorice of 4%, 4% Siberian cocklebur extract, the black nightshade extract of 4%, the sucrose fatty acid ester of 3% and sucrose ester trimerization qualities of glycerin to mix than the water for the mixture of 1:1, dispersant and remainder and naturally cool to room temperature after 38min is boiled in heating and obtain culture fluid B;

D 45 DEG C of placement more than 48h after culture fluid A and culture fluid B mixing and stirring can be obtained required biopesticide finished product by ().

2013-2014 years, researcher tests in Yangcheng Lake area, Suzhou, Jiangsu Province with choosing a green cucumber, be divided into two pieces, the applications of pesticide of control group adopt traditional formulation of pesticide, the biopesticide that experimental group adopts the embodiment of the present invention 4 to make, experimental group and control group, other plantations, irrigate, way to manage is all identical, carry out an acceptance inspection when within 2014, cucumber is ripe, it is 96% that experimental group cucumber downy mildew prevents and treats efficiency, and the cucumber downy mildew of control group to prevent and treat efficiency be 70%, experimental group comparatively control group cucumber yield increases by 25%; In addition, biopesticide prepared by the present invention is nontoxic, ecological, environmental protective, significantly reduces dosage, spraying times and drug cost, and preparation is convenient, and the holding time is long, has very strong practical value.

It should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention, can also make some improvement, these improvement also should be considered as protection scope of the present invention.

Claims

1. one kind is applicable to the biopesticide of cucumber downy mildew, it is characterized in that the component comprising following percentage by weight: the bacillus subtilis wetting powder of the monad 200-300 times dilution of 5%-10%, the catechin 300-500 times of dilution of 10%-15%, 10%-25%, the kasugarnycin wetting powder of 5%-15%, the licorice of 3%-5%, the Siberian cocklebur extract of 3%-5%, the black nightshade extract of 3%-5%, the emulsifier of 1%-5%, the dispersant of 1%-5%, water surplus.

2. the biopesticide being applicable to cucumber downy mildew according to claim 1, is characterized in that: described emulsifier is one or both in sucrose fatty acid ester and sucrose ester three polyglycereol.

3. the biopesticide being applicable to cucumber downy mildew according to claim 1, is characterized in that: described dispersant is fatty acid dispersant.

4. the biopesticide being applicable to cucumber downy mildew according to claim 1, is characterized in that: described licorice, Siberian cocklebur extract and black nightshade extract are water extract.

5. the preparation method being applicable to the biopesticide of cucumber downy mildew according to claim 1, is characterized in that comprising the following steps:

6. the preparation method being applicable to the biopesticide of cucumber downy mildew according to claim 5, is characterized in that: in described step (b), agitating mode should at the uniform velocity stir in same direction.