CN105176956A - Additive for reducing release of harmful ingredients in reconstituted tobacco and preparation method and application of additive - Google Patents
Additive for reducing release of harmful ingredients in reconstituted tobacco and preparation method and application of additive Download PDFInfo
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- CN105176956A CN105176956A CN201510487697.4A CN201510487697A CN105176956A CN 105176956 A CN105176956 A CN 105176956A CN 201510487697 A CN201510487697 A CN 201510487697A CN 105176956 A CN105176956 A CN 105176956A
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B3/00—Preparing tobacco in the factory
- A24B3/14—Forming reconstituted tobacco products, e.g. wrapper materials, sheets, imitation leaves, rods, cakes; Forms of such products
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/01—Carboxylic ester hydrolases (3.1.1)
- C12Y301/01011—Pectinesterase (3.1.1.11)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y402/00—Carbon-oxygen lyases (4.2)
- C12Y402/02—Carbon-oxygen lyases (4.2) acting on polysaccharides (4.2.2)
- C12Y402/02002—Pectate lyase (4.2.2.2)
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Abstract
The invention discloses an additive for reducing the release of harmful ingredients in reconstituted tobacco and a preparation method and application of the additive. The additive is made mainly from cellulase, hemicellulase, amylase, pectinase, protease and biological saccharidase. The raw materials are mixed and stirred proportionally to obtain the additive. Raw materials of the reconstituted tobacco such as tobacco stem and tobacco waste are extracted and separated to obtain residue material, the residue material is pulped to obtain pulp, the concentration of the pulp is adjusted to a range of 3-35%, the additive is then added into the pulp and made into reconstituted tobacco base sheets, and the base sheets are coated, dried and torn into finished reconstituted tobacco. The release of the harmful ingredients in the reconstituted tobacco can be lowered significantly.
Description
Technical field
The present invention relates to reconstituted tobacco additive, be specifically related to a kind of biotic additives and its preparation method and application.
Background technology
The production of papermaking-method reconstituted tobaccos with tobacco leaf fragment, offal and offal for main raw material, slurrying in extracting technology and paper-making technique in integrated application chemical and medicine industry technology, the critical process such as to manufacture paper with pulp, and the reconstituted tobacco taking machinery and physical method to process to have similar Redried lamina physical parameter and water ratio etc.
Reconstituted tobacco is applied to the history that cigarette composition has more than 60 year abroad.As far back as 1949, RJ Reynolds Tobacco Holdings ,Inc. of the U.S. just added reconstituted tobacco in produced cigarette, becomes the cigarette enterprise using technique in the world the earliest.Current French Mo Di company is the enterprise that reconstituted tobacco output is maximum in the world, and its technique research and development is taken its place in the front ranks of the world always, the product produced, and no matter is inner quality or physical index, is all in the leading level in the world.The industrialization research and development of China's reconstituted tobacco, start from the later stage nineties in last century.Continuous enforcement and being completed along with reconstituted tobacco construction project is gone into operation and reconstituted tobacco is applied to cigarette composition and continuous increase ratio by domestic cigarette enterprise successively, and output and the usage quantity of domestic reconstituted tobacco increase year by year.
Because reconstituted tobacco raw material sources are extensive, the factor impacts such as defect own is as large in assorted gas, fiber taste, objectively need to improve or cover, also cause objectionable constituent burst size to increase as prussic acid and ammonia etc., therefore reconstituted tobacco manufacturing enterprise will use a large amount of additives in its product.With regard to cigaret additive, countries in the world all take much count of its research.Comparatively speaking, China is relatively weak in cigaret additive industrial system, cigaret additive poor product quality, the kind Gong selecting is more limited, moreover these additive major controls are in supplier's hand, and the overwhelming majority is synthetics, product composition is indefinite, and quality is unstable.For a long time, this is a bottleneck problem of restriction Cigarette Industrial Enterprise.Although the burst size of prussic acid, ammonia is not high in reconstituted tobacco main flume, the content compared in whole flue gas seems insignificant, and its hazardness can not be ignored, and is subject to concern and the attention of numerous scientific workers, also becomes the focus and emphasis of research.
Summary of the invention
The object of this invention is to provide a kind of reconstituted tobacco additive and its preparation method and application.Additive principal character of the present invention reduces objectionable constituent burst size in reconstituted tobacco, reconstituted tobacco flavor and taste can be made to improve simultaneously, and fragrance is coordinated, pleasant impression is comfortable, flue gas is fine and smooth, soft, effectively reduces flue gas to the pungent stimulation of smoker's respiratory system, alleviates harm of smoking.
Object of the present invention is achieved through the following technical solutions:
Prepare reconstituted tobacco additive main raw material used and consumption with parts by weight is:
Cellulase: 20 ~ 60 parts; Hemicellulase: 20 ~ 60 parts; Amylase: 1 ~ 20 part; Polygalacturonase: 2 ~ 20% parts; Proteolytic enzyme: 5 ~ 30 parts; Biological carbohydrase: 1 ~ 10% part.
A kind of preferred version reducing the additive of objectionable constituent burst size in reconstituted tobacco is: its main raw material and consumption thereof with parts by weight are: cellulase 30 ~ 40 parts; Hemicellulase 20 ~ 30 parts; Amylase 5 ~ 10 parts; Polygalacturonase 10 ~ 15 parts; 5 ~ 10 parts, proteolytic enzyme; Biological carbohydrase 5 ~ 8 parts.
The present invention most preferably scheme is: its main raw material and consumption are by weight percentage:
30% cellulase, 20% hemicellulase, 10% amylase, 15% polygalacturonase, 20% proteolytic enzyme and 5% biological carbohydrase.
Invention also provides a kind of preparation method reducing the additive of objectionable constituent burst size in reconstituted tobacco:
Proportionally, precise cellulase, hemicellulase, amylase, polygalacturonase, proteolytic enzyme and biological carbohydrase, be placed in mixer and mix, stir, store for future use, and is reconstituted tobacco additive.
Invention also provides a kind of application reducing the additive of objectionable constituent burst size in reconstituted tobacco:
By reconstituted tobacco raw materials for production as offal, offal etc., obtain slag charge through abstraction process and separation circuit, slag charge obtains slurry through defibrination operation, slurry concentration adjustment in 3 ~ 25% scopes, again reconstituted tobacco additive is added in slurry, manufacture paper with pulp into reconstituted tobacco substrate by the equipment of manufacturing paper with pulp.Substrate through painting process, baking operation, tear operation after the finished product that obtains, be reconstituted tobacco.The additive ratio of reconstituted tobacco is that the thousandth of reconstituted tobacco raw material is to one of percentage scope.
Embodiment
The present invention will further describe as follows by embodiment, but the scope of the invention is not limited to these embodiments certainly.
Embodiment one
Prepare the raw material of reconstituted tobacco in the offal ratio of the 60% broken end of tobacco leaf and 40%, raw material obtains slag charge through hot water extraction, solid-liquid separation, and slag charge through defibrination, and slurry concentration adjustment in 15% scope, manufactures paper with pulp into reconstituted tobacco substrate by the equipment of manufacturing paper with pulp.Substrate through painting process, baking operation, tear operation after the finished product that obtains, be reconstituted tobacco, be 1# and contrast tobacco leaf.
Embodiment two
First prepare the raw material of reconstituted tobacco in the offal ratio of the 60% broken end of tobacco leaf and 40%, raw material obtains slag charge through hot water extraction, solid-liquid separation, slag charge through defibrination, and slurry concentration adjustment in 15% scope.Next is the weighing carrying out reconstituted tobacco additive in 30% cellulase, 20% hemicellulase, 10% amylase, 15% polygalacturonase, 20% proteolytic enzyme and 5% biological carbohydrase ratio, and is placed in mixer, stirs, stores for future use.Be finally the ratio of 5/1000ths in reconstituted tobacco additive than reconstituted tobacco raw material, reconstituted tobacco additive is added in slurry, reconstituted tobacco substrate is manufactured paper with pulp into by the equipment of manufacturing paper with pulp, substrate through painting process, baking operation, tear operation after the finished product that obtains, be reconstituted tobacco, be 2# and test tobacco leaf.
Embodiment three
First prepare the raw material of reconstituted tobacco in the offal ratio of the 60% broken end of tobacco leaf and 40%, raw material obtains slag charge through hot water extraction, solid-liquid separation, slag charge through defibrination, and slurry concentration adjustment in 15% scope.Next is the weighing carrying out reconstituted tobacco additive in 20% cellulase, 30% hemicellulase, 15% amylase, 15% polygalacturonase, 15% proteolytic enzyme and 5% biological carbohydrase ratio, and is placed in mixer, stirs, stores for future use.Be finally the ratio of 5/1000ths in reconstituted tobacco additive than reconstituted tobacco raw material, reconstituted tobacco additive is added in slurry, reconstituted tobacco substrate is manufactured paper with pulp into by the equipment of manufacturing paper with pulp, substrate through painting process, baking operation, tear operation after the finished product that obtains, be reconstituted tobacco, be 3# and test tobacco leaf.
Embodiment four
First prepare the raw material of reconstituted tobacco in the offal ratio of the 60% broken end of tobacco leaf and 40%, raw material obtains slag charge through hot water extraction, solid-liquid separation, slag charge through defibrination, and slurry concentration adjustment in 15% scope.Next is the weighing carrying out reconstituted tobacco additive in 20% cellulase, 20% hemicellulase, 20% amylase, 15% polygalacturonase, 20% proteolytic enzyme and 5% biological carbohydrase ratio, and is placed in mixer, stirs, stores for future use.Be finally the ratio of 5/1000ths in reconstituted tobacco additive than reconstituted tobacco raw material, reconstituted tobacco additive is added in slurry, reconstituted tobacco substrate is manufactured paper with pulp into by the equipment of manufacturing paper with pulp, substrate through painting process, baking operation, tear operation after the finished product that obtains, be reconstituted tobacco, be 4# and test tobacco leaf.
Substrate detected result in embodiment is in table 1.
The detected result of table 1 reconstituted tobacco substrate
Note: hazardness index=(CO/14.2+HCN/146.3+NNK/5.5+NH3/8.1+B [a] P/10.9+ phenol/17.4+ crotonic aldehyde/18.6) * 10/7
Analyze above detected result, we can obtain following conclusion:
1, prussic acid burst size significantly reduces
Compared with contrasting tobacco leaf with 1#, the 2# prussic acid burst size tested in tobacco leaf flue gas reduces by 32.5%, 3# tests the prussic acid burst size reduction by 32.5% in tobacco leaf flue gas, the prussic acid burst size in 4# test tobacco leaf flue gas reduces by 32.5%.
2, ammonia burst size significantly reduces
Compared with contrasting tobacco leaf with 1#, the 2# ammonia burst size tested in tobacco leaf flue gas reduces by 61.6%, 3# tests the ammonia burst size reduction by 49.31% in tobacco leaf flue gas, the ammonia burst size in 4# test tobacco leaf flue gas reduces by 53.4%.
3, hazardness index reduces
Compared with contrasting tobacco leaf with 1#, the overall hazardness index reduction by 17.1% of 2# test tobacco leaf flue gas, 3# test the overall hazardness index reduction by 18.5% of tobacco leaf flue gas, the overall hazardness index reduction by 5.7% of 4# test tobacco leaf flue gas.
Test-results shows, the reconstituted tobacco additive be made up of cellulase, hemicellulase, amylase, polygalacturonase, proteolytic enzyme and biological carbohydrase can reduce objectionable constituent burst size in reconstituted tobacco significantly, reconstituted tobacco flavor and taste can be made to improve simultaneously, fragrance is coordinated, pleasant impression is comfortable, flue gas is fine and smooth, soft, effectively reduces flue gas to the pungent stimulation of smoker's respiratory system, alleviates harm of smoking.
Claims (7)
1. reduce an additive for objectionable constituent burst size in reconstituted tobacco, its main raw material and consumption thereof with parts by weight are: cellulase 20 ~ 60 parts; Hemicellulase 20 ~ 60 parts; Amylase 1 ~ 20 part; Polygalacturonase 2 ~ 20 parts; 5 ~ 30 parts, proteolytic enzyme; Biological carbohydrase 1 ~ 10 part.
2. reduce an additive for objectionable constituent burst size in reconstituted tobacco, its main raw material and consumption thereof with parts by weight are: cellulase 30 ~ 40 parts; Hemicellulase 20 ~ 30 parts; Amylase 5 ~ 10 parts; Polygalacturonase 10 ~ 15 parts; 5 ~ 10 parts, proteolytic enzyme; Biological carbohydrase 5 ~ 8 parts.
3. additive as claimed in claim 1, its main raw material and consumption are by weight percentage:
30% cellulase, 20% hemicellulase, 10% amylase, 15% polygalacturonase, 20% proteolytic enzyme and 5% biological carbohydrase.
4. as the preparation method of the additive as described in arbitrary in claim 1-3, it comprises the steps: precise cellulase, hemicellulase, amylase, polygalacturonase, proteolytic enzyme and biological carbohydrase, is placed in mixer, stirs, store for future use, be reconstituted tobacco additive.
5. as the application of the additive as described in arbitrary in claim 1-3, it is characterized in that: by reconstituted tobacco raw materials for production as offal, offal etc., obtain slag charge through abstraction process and separation circuit, slag charge obtains slurry through defibrination operation, slurry concentration adjustment in 3 ~ 25% scopes, again reconstituted tobacco additive is added in slurry, manufacture paper with pulp into reconstituted tobacco substrate by the equipment of manufacturing paper with pulp; Substrate through painting process, baking operation, tear operation after the finished product that obtains, be reconstituted tobacco; The additive ratio of reconstituted tobacco is that the thousandth of reconstituted tobacco raw material is to one of percentage scope.
6. the application of additive as claimed in claim 5, it is characterized in that: first prepare the raw material of reconstituted tobacco in the offal ratio of the 60% broken end of tobacco leaf and 40%, raw material obtains slag charge through hot water extraction, solid-liquid separation, and slag charge through defibrination, and adjusts slurry concentration in 15% scope; Next is the weighing carrying out reconstituted tobacco additive in 30% cellulase, 20% hemicellulase, 10% amylase, 15% polygalacturonase, 20% proteolytic enzyme and 5% biological carbohydrase ratio, and is placed in mixer, stirs, stores for future use; Be finally the ratio of 5/1000ths in reconstituted tobacco additive than reconstituted tobacco raw material, reconstituted tobacco additive is added in slurry, manufacture paper with pulp into reconstituted tobacco substrate by the equipment of manufacturing paper with pulp, substrate through painting process, baking operation, tear operation after the finished product that obtains, be reconstituted tobacco.
7. the application of additive as claimed in claim 6, it is characterized in that: objectionable constituent burst size in reconstituted tobacco can be reduced significantly, reconstituted tobacco flavor and taste can be made to improve simultaneously, fragrance is coordinated, pleasant impression is comfortable, flue gas is fine and smooth, soft, effectively reduces flue gas to the pungent stimulation of smoker's respiratory system, alleviates harm of smoking.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510487697.4A CN105176956A (en) | 2015-08-11 | 2015-08-11 | Additive for reducing release of harmful ingredients in reconstituted tobacco and preparation method and application of additive |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510487697.4A CN105176956A (en) | 2015-08-11 | 2015-08-11 | Additive for reducing release of harmful ingredients in reconstituted tobacco and preparation method and application of additive |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108823190A (en) * | 2018-06-06 | 2018-11-16 | 云南中烟工业有限责任公司 | A kind of complex enzyme formulation and its preparation method and application reducing typical nitrosamine of tobacco leaves |
Citations (3)
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|---|---|---|---|---|
| CN1246307A (en) * | 1998-08-31 | 2000-03-08 | 朱大恒 | Method for making tobacco sheet |
| CN101194753A (en) * | 2007-06-21 | 2008-06-11 | 深圳市洲际伟业科技发展有限公司 | Tobacco biological carbohydrase for improving flavour absorbing quality of tobacco |
| CN102396780A (en) * | 2011-07-12 | 2012-04-04 | 广西中烟工业有限责任公司 | Selective harm-reducing cigarette and preparation method thereof |
-
2015
- 2015-08-11 CN CN201510487697.4A patent/CN105176956A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1246307A (en) * | 1998-08-31 | 2000-03-08 | 朱大恒 | Method for making tobacco sheet |
| CN101194753A (en) * | 2007-06-21 | 2008-06-11 | 深圳市洲际伟业科技发展有限公司 | Tobacco biological carbohydrase for improving flavour absorbing quality of tobacco |
| CN102396780A (en) * | 2011-07-12 | 2012-04-04 | 广西中烟工业有限责任公司 | Selective harm-reducing cigarette and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| CHUAN LIU ET AL.: "The use of a novel tobacco treatment process to reduce toxicant yields in cigarette smoke.", 《FOOD AND CHEMICAL TOXICOLOGY》 * |
| 宋凌勇等: "应用生物酶制剂改善再造烟叶内在品质的研究", 《广西农学报》 * |
| 李小斌等: "生物技术在辊压法烟草薄片中的应用研究", 《河南科技》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108823190A (en) * | 2018-06-06 | 2018-11-16 | 云南中烟工业有限责任公司 | A kind of complex enzyme formulation and its preparation method and application reducing typical nitrosamine of tobacco leaves |
| CN108823190B (en) * | 2018-06-06 | 2021-09-14 | 云南中烟工业有限责任公司 | Complex enzyme preparation for reducing specific nitrosamines in tobacco leaves and preparation method and application thereof |
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Application publication date: 20151223 |