CN105176821A - Preparation method of medicinal plant balloonflower growth-promoting microbial inoculant - Google Patents
Preparation method of medicinal plant balloonflower growth-promoting microbial inoculant Download PDFInfo
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- CN105176821A CN105176821A CN201510589197.1A CN201510589197A CN105176821A CN 105176821 A CN105176821 A CN 105176821A CN 201510589197 A CN201510589197 A CN 201510589197A CN 105176821 A CN105176821 A CN 105176821A
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Abstract
The invention discloses a preparation method of a medicinal plant balloonflower growth-promoting microbial inoculant, which comprises the following steps: adding distilled water to dissolve rotten grass carbon, and taking the supernatant for later use; putting the supernatant into a glass container, adding a sodium hydroxide solution to regulate the pH value, and carrying out high-temperature sterilization; activating the separately preserved plant growth promoting bacterium, inoculating into the sterilized rotten grass carbon solution, and culturing in a heating centrifuge for 48 hours to obtain a liquid strain; transferring the liquid strain into a fermentation tank, adding humic acid, and carrying out inoculation culture for 36 hours; and thoroughly mixing the liquid strain subjected to inoculation culture with powdered charcoal in a ratio of 1:6, and carrying out aeration drying to obtain the solid growth-promoting microbial inoculant. Compared with the prior art, the growth-promoting microbial inoculant can promote the absorption and utilization of the rhizosphere nutrients by the balloonflower, effectively control multiple plant diseases, reduce the consumptions of the pesticide and chemical fertilizer, protect the environment, reduce the pollution, improve the farmland quality and enhance the balloonflower growth efficiency, thereby having popularization and application value.
Description
Technical field
The present invention relates to a kind of biological inoculum preparation method, particularly relate to a kind of preparation method of medicinal plant balloonflower root growth promoting bacteria agent.
Background technology
Balloonflower root, another name burden flower, small bell flower, mitral flower, be per nnial herb, stem is high 20 ~ 120 centimetres, usually without hair, even close by undercoat, not branch, few top branch.Leaf is all verticillate, and part is verticillate to whole alternate, stockless or have extremely short handle, and blade is avette, and ovum shape is oval to lanceolar, the avette or ovum shape lanceolar of leaf, and flower faint blue or dark violet white, can do ornamental flower; Its root can be used as medicine, and has cough-relieving apophlegmatic, effect such as a surname lung, apocenosis etc., traditional Chinese medical science common medicine.Balloonflower root too much relies in producing and uses chemical fertilizer easily to produce the continuous cropping obstacles such as disease and pest, in the prior art, the growth promoting bacteria agent with disease resistance is more, its preparation method is also different, but do not see the growth promoting bacteria agent that balloonflower root is produced, also have no relevant report, therefore, need a kind of growth promoting bacteria agent for balloonflower root to be born.
Summary of the invention
Object of the present invention is just the preparation method providing a kind of medicinal plant balloonflower root growth promoting bacteria agent in order to solve the problem.
The present invention is achieved through the following technical solutions above-mentioned purpose:
The present invention includes following steps:
(1) peat composed of rotten mosses that will become thoroughly decomposed adds distilled water and dissolves, and ratio is 1:10, leaves standstill that to get supernatant liquor after 12 hours for subsequent use after dissolving;
(2) insert in Glass Containers by supernatant liquor, the sodium hydroxide solution adding 5% regulates pH to be for subsequent use after 6.5 ~ 6.8;
(3) solution prepared by step (2) is carried out the high-temperature sterilization 5 ~ 8 minutes of 100 DEG C ~ 110 DEG C;
(4) by the plant growth-promoting bacteria of separating and preserving activation after access sterilizing after become thoroughly decomposed in peat composed of rotten mosses solution, in 40 DEG C ~ 45 DEG C in solution, in the heating whizzer of rotating speed 80 ~ 90 revs/min cultivate within 48 hours, obtain liquid spawn;
(5) being proceeded to by the liquid spawn that step (4) obtains in fermentor tank and to add humic acid, humic acid accounting example is 1% ~ 3%, carries out inoculation culture 36 hours;
(6) liquid spawn after inoculation being cultivated fully mixes with wood charcoal powder, and ratio is 1:6, obtains solid-state growth promoting bacteria agent after air seasoning.
Beneficial effect of the present invention is:
The present invention is a kind of preparation method of medicinal plant balloonflower root growth promoting bacteria agent; compared with prior art; the present invention can promote that balloonflower root is to the absorption of root system nutrient and utilization; and can effectively preventing plurality of plant diseases, the usage quantity of agricultural chemicals and chemical fertilizer can be reduced, protection of the environment; decreasing pollution; improve farm land quality, improve the growth efficiency of balloonflower root, there is the value applied.
Embodiment
The invention will be further described below:
The present invention includes following steps:
(1) peat composed of rotten mosses that will become thoroughly decomposed adds distilled water and dissolves, and ratio is 1:10, leaves standstill that to get supernatant liquor after 12 hours for subsequent use after dissolving;
(2) insert in Glass Containers by supernatant liquor, the sodium hydroxide solution adding 5% regulates pH to be for subsequent use after 6.5 ~ 6.8;
(3) solution prepared by step (2) is carried out the high-temperature sterilization 5 ~ 8 minutes of 100 DEG C ~ 110 DEG C;
(4) by the plant growth-promoting bacteria of separating and preserving activation after access sterilizing after become thoroughly decomposed in peat composed of rotten mosses solution, in 40 DEG C ~ 45 DEG C, in the heating whizzer of rotating speed 80 ~ 90 revs/min cultivate within 48 hours, obtain liquid spawn;
(5) being proceeded to by the liquid spawn that step (4) obtains in fermentor tank and to add humic acid, humic acid accounting example is 1% ~ 3%, carries out inoculation culture 36 hours;
(6) liquid spawn after inoculation being cultivated fully mixes with wood charcoal powder, and ratio is 1:6, obtains solid-state growth promoting bacteria agent after air seasoning.
When the present invention uses, by the solid-state growth promoting bacteria agent obtained (conventional is granulated solid), apply the balloonflower root rhizosphere soil in farmland, the average every strain balloonflower root about 1 gram of amount of application in seedling stage, easy to use, implementation method is simple.
More than show and describe ultimate principle of the present invention and principal character and advantage of the present invention.The technician of the industry should understand; the present invention is not restricted to the described embodiments; what describe in above-described embodiment and specification sheets just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.Application claims protection domain is defined by appending claims and equivalent thereof.
Claims (1)
1. a preparation method for medicinal plant balloonflower root growth promoting bacteria agent, is characterized in that, comprises the following steps:
(1) peat composed of rotten mosses that will become thoroughly decomposed adds distilled water and dissolves, and ratio is 1:10, leaves standstill that to get supernatant liquor after 12 hours for subsequent use after dissolving;
(2) insert in Glass Containers by supernatant liquor, the sodium hydroxide solution adding 5% regulates pH to be for subsequent use after 6.5 ~ 6.8;
(3) solution prepared by step (2) is carried out the high-temperature sterilization 5 ~ 8 minutes of 100 DEG C ~ 110 DEG C;
(4) by the plant growth-promoting bacteria of separating and preserving activation after access sterilizing after become thoroughly decomposed in peat composed of rotten mosses solution, in 40 DEG C ~ 45 DEG C, in the heating whizzer of rotating speed 80 ~ 90 revs/min cultivate within 48 hours, obtain liquid spawn;
(5) being proceeded to by the liquid spawn that step (4) obtains in fermentor tank and to add humic acid, humic acid accounting example is 1% ~ 3%, carries out inoculation culture 36 hours;
(6) liquid spawn after inoculation being cultivated fully mixes with wood charcoal powder, and ratio is 1:6, obtains solid-state growth promoting bacteria agent after air seasoning.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510589197.1A CN105176821A (en) | 2015-09-17 | 2015-09-17 | Preparation method of medicinal plant balloonflower growth-promoting microbial inoculant |
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| CN201510589197.1A CN105176821A (en) | 2015-09-17 | 2015-09-17 | Preparation method of medicinal plant balloonflower growth-promoting microbial inoculant |
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| CN105176821A true CN105176821A (en) | 2015-12-23 |
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Citations (5)
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|---|---|---|---|---|
| CN101230322A (en) * | 2007-09-11 | 2008-07-30 | 南开大学 | Novel medical stone micro-ecological repairing additive and preparation method thereof |
| CN102321554A (en) * | 2011-08-30 | 2012-01-18 | 新疆农业科学院微生物应用研究所 | Bacillus cereus and its application of being as plant growth-promoting rhizobacteria |
| CN102409014A (en) * | 2011-11-23 | 2012-04-11 | 山东省林业科学研究院 | Ziziphus jujube mill photosphere rhizosphere bacillus sp. capable of promoting growth |
| CN103820348A (en) * | 2012-11-16 | 2014-05-28 | 中国科学院生态环境研究中心 | Plant growth-promoting bacteria and preparation method and application of fungicide |
| CN103897996A (en) * | 2012-12-26 | 2014-07-02 | 魏明重 | Producing method of agricultural halotolerant bacteria |
-
2015
- 2015-09-17 CN CN201510589197.1A patent/CN105176821A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101230322A (en) * | 2007-09-11 | 2008-07-30 | 南开大学 | Novel medical stone micro-ecological repairing additive and preparation method thereof |
| CN102321554A (en) * | 2011-08-30 | 2012-01-18 | 新疆农业科学院微生物应用研究所 | Bacillus cereus and its application of being as plant growth-promoting rhizobacteria |
| CN102409014A (en) * | 2011-11-23 | 2012-04-11 | 山东省林业科学研究院 | Ziziphus jujube mill photosphere rhizosphere bacillus sp. capable of promoting growth |
| CN103820348A (en) * | 2012-11-16 | 2014-05-28 | 中国科学院生态环境研究中心 | Plant growth-promoting bacteria and preparation method and application of fungicide |
| CN103897996A (en) * | 2012-12-26 | 2014-07-02 | 魏明重 | Producing method of agricultural halotolerant bacteria |
Non-Patent Citations (3)
| Title |
|---|
| ADUNGO BENARD ANGALU: "Evaluation of soil extracts as a culture medium for the growth of bacteria", 《（MICROBIOLOGY AND BIOTECHNOLOGY）DEGREE PROGRAM, UNIVERSITY OF NAIROBI》 * |
| I.J希金斯等: "《生物技术原理和应用》", 30 November 1989 * |
| 闵航: "《微生物学》", 30 June 2011, 浙江大学出版社 * |
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Application publication date: 20151223 |
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