CN105168537A - Compound medicine for treating bone fracture and preparation method of compound medicine - Google Patents
Compound medicine for treating bone fracture and preparation method of compound medicine Download PDFInfo
- Publication number
- CN105168537A CN105168537A CN201510580362.7A CN201510580362A CN105168537A CN 105168537 A CN105168537 A CN 105168537A CN 201510580362 A CN201510580362 A CN 201510580362A CN 105168537 A CN105168537 A CN 105168537A
- Authority
- CN
- China
- Prior art keywords
- radix
- moringa
- leaf
- powder
- parts
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to a compound medicine for treating bone fracture and a preparation method of the compound medicine. The compound medicine is prepared from a horseradish tree leaf extract, astragalus membranaceus, codonopsis pilosula, carthamus tinctorius, ligusticum wallichii, angelica sinensis, rehmannia glutinosa, cucumber seeds, pseudo-ginseng and liquorice in forms of decoction, granule, powder, capsule, pill or tablet. Repeated verification of clinical application shows that the compound medicine has effective and remarkable treatment advantages of enriching blood and supplementing calcium, tonifying and enriching qi, activating blood and stimulating menstrual flow, invigorating blood circulation and removing stasis, strengthening body resistance and strengthening tendons and bones, and has functions of accelerating bone mineral deposition rate, increasing bone density and promoting fracture side heal.
Description
Technical field
The invention belongs to the field of Chinese medicines, particularly relate to a kind of compound medicine for the treatment of fracture and preparation method thereof.
Background technology
Fracture is that the integrity of phalanges or seriality are damaged caused, the disease being main manifestations with pain, swelling, livid purple, dysfunction, deformity and bony crepitus etc.Its clinical main manifestations is: fractures has localized pain and tenderness, local swelling and occur ecchymosis, limb function position or completely lose, and completeness sclerotin still can occur cacomelia and abnormal movement.Fracture is a kind of commonly encountered diseases, is the clinical frequently-occurring disease of orthopaedic trauma, can damages social labor power, the health of the serious harm mankind.According to investigations, the prevalence of China's fracture is about 1.4%, and because China's population base is comparatively large, the fracture patient of existence is also more.Although the Chinese medicine of current treatment this respect disease is a lot, but majority also exists, and reducing swelling and alleviating pain is slow, the course for the treatment of is long, the dissatisfactory shortcoming of effect, doctor trained in Western medicine is when treating the comparatively serious bone injury of this respect and being sick, normally by the position of bone righting of patient part, then medical apparatus and instruments is used to be carried out internal fixtion, or carry out extenal fixation, resting slowly with Gypsum Fibrosum, binder, clamping plate etc.This western medicine therapy utilizing operation and medicine, weak point is that sufferer healing recovers comparatively slow, and treatment cycle is longer, and medical expense is high, and in process, patient is more painful.If meet the patient of unsuitable operative treatment, this Therapeutic Method just has certain limitation.
Leaf of Moringa refers to the leaves of the perennial tropical deciduous tree Moringa tree (being also called drumstick tree) originating in India, it is a kind of high calcium plant product, calcium content is more than 4 times of milk, in addition, also containing the very high trace element such as potassium, ferrum, zinc, containing following the much the same protein of Semen sojae atricolor and aminoacid etc., it is a kind of functional food containing fully nutrient, as long as a people is able to eat about 5 grams every day and can supplies the human body nutritional need of a day, therefore it can replenish the calcium, iron supplement, kalium replenishment and zinc supplement.The tender leaf of Moringa of the selected children of leaf of Moringa powder, forms through micronizing, has comprehensive extra-nutrition, improves immunity, eliminating toxin and beautifying the skin, and logical intestinal is convenient, the effects such as fat-reducing.Leaf of Moringa powder is nutritious and comprehensive, and vitamin E is 70 times and 40 times of spirulina and analysis for soybean powder respectively.Moringa has " miracle tree ", the title of " mother's ace buddy " and " medical treasure case ".In Africa, it is called as the cattle milk of the poor; In India, Moringa has had the edible history of more than one thousand years, has become one of indispensable food of daily life; Be called in China Taiwan " 21 century human body bodyguard ".So far bibliographical information leaf of Moringa or the total flavones of Moringa oleifera leaf purposes in fracture is not had.
Containing flavone compound in leaf of Moringa, the physiologically active of flavone compound is comparatively extensive, has scavenging free radicals, antioxidation, anticancer, anti-cancer, effect such as treatment cardiovascular diseases, blood sugar lowering etc.
Chinese invention patent 201310238201.0 discloses a kind of Chinese medicine composition for the treatment of fracture, and the raw materials of this Chinese medicine composition comprises: Semen Strychni 10-30 part, Herba Artemisiae Anomalae 10-40 part, Radix Astragali 10-50 part, Cortex Eucommiae 10-20 part, Radix Lamiophlomidis Rotatae 5-10 part, Rhizoma Chuanxiong 5-15 part, Radix Rehmanniae Preparata 5-15 part, Radix Notoginseng 5-15 part, Eupolyphaga Seu Steleophaga 5-15 part, Olibanum 9-15 part, Myrrha 9-15 part, Rhizoma Drynariae 5-15 part, Fructus Chaenomelis 3-8 part and Radix Glycyrrhizae 1.5-9 part.This Chinese medicine composition has effect of kidney tonifying synthetism, expelling pus and promoting granulation, reducing swelling and alleviating pain and heat-clearing and toxic substances removing, but at benefiting QI for activating blood circulation, blood nourishing and calcium replenishing aspect effect is not very remarkable.
Summary of the invention
The object of the invention is, there is provided a kind of compound medicine for the treatment of fracture and preparation method thereof, this compound medicine makes decoction, granule, powder, capsule, pill or tablet by moringa oleifera leaf extractive, the Radix Astragali, Radix Codonopsis, Flos Carthami, Rhizoma Chuanxiong, Radix Angelicae Sinensis, Radix Rehmanniae Preparata, Semen Cucumidis sativi, Radix Notoginseng and Radix Glycyrrhizae.Shown by the checking repeatedly of clinical practice, this compound medicine effective blood nourishing and calcium replenishing, QI invigorating QI invigorating, promoting blood circulation to restore menstrual flow, promoting blood circulation to remove blood stasis, the body resistance strengthening and constitution consolidating and bone and muscle strengthening etc. can treat advantage significantly.Have and accelerate bone ore deposit sedimentation rate, improve bone density, promote the function of fracture site healing.
A kind of compound medicine for the treatment of fracture of the present invention, make decoction, granule, powder, capsule, pill or tablet by crude drug moringa oleifera leaf extractive 5-30 part, Radix Astragali 6-30 part, Radix Codonopsis 9-30 part, Flos Carthami 3-20 part, Rhizoma Chuanxiong 5-30 part, Radix Angelicae Sinensis 6-20 part, Radix Rehmanniae Preparata 9-15 part, Semen Cucumidis sativi 3-20 part, Radix Notoginseng 3-20 part and Radix Glycyrrhizae 1-15 part, concrete operations follow these steps to carry out:
Preparation moringa oleifera leaf extractive:
A, take leaf of Moringa, dry 2.5h under temperature 50 C, pulverize, cross 45 mesh sieves, obtain leaf of Moringa powder;
B, get the leaf of Moringa powder that step a obtains, add the petroleum ether of leaf of Moringa opaque amount volume 3 times, reflux defat 3 times at temperature 68 DEG C, each 6h, crosses elimination filtrate, by filter residue and drying, obtain dry filtering residue;
The concentration adding filtering residue quality 12 times of volumes in c, the dry filtering residue that obtained by step b is 70% ethanol, 7min is processed under microwave power 600W, be placed in temperature 80 DEG C of tepidarium supersound extraction 2 times, each extraction 25min, filtration obtains extracting solution, merge extractive liquid, obtains total flavones of Moringa oleifera leaf crude extract;
D, the crude extract obtained in step c is joined in AB-8 type macroporous adsorptive resins, gradient elution is carried out successively with the ethanol that the distilled water of 2 times of column volumes and the volume fraction of 3 times of column volumes are respectively 30%, 50%, 70%, adjusted volume flow is 2mL/min, often 15min collected by pipe, with NaNO
2-Al (NO
3)
3-NaOH system color developing detection, under wavelength is 510nm, measure absorbance inspection know, collect the ethanol elution of 50% and 70%, merge eluent, eluent decompression recycling ethanol is concentrated into 1/5 of original volume, and obtain concentrated solution, concentrated solution being placed in temperature 60 C water-bath evaporate to dryness to relative density is the extractum of 1.25, dry at 50 DEG C, obtain moringa oleifera leaf extractive total flavones powder;
Prepare compound medicines:
E, take the Radix Astragali, Radix Codonopsis, Flos Carthami, Rhizoma Chuanxiong, Radix Angelicae Sinensis, Radix Rehmanniae Preparata, Semen Cucumidis sativi, Radix Notoginseng and Radix Glycyrrhizae, be placed in water respectively and soak 20-30 minute, decoct with water 2-3 time, amount of water is 5-8 times that soaks water consumption again, each decoction 30-90 minute, filter, obtain filtrate, merging filtrate, when being evaporated to temperature 80 DEG C, relative density is the extractum of 1.25-1.30, drying under reduced pressure, is ground into fine powder;
F, moringa oleifera leaf extractive total flavones powder steps d obtained mix with the fine powder that step e obtains, and stir, and must treat the compound medicine of fracture.
The compound medicine of described treatment fracture, makes decoction, granule, powder, capsule, pill or tablet by crude drug moringa oleifera leaf extractive 10-20 part, Radix Astragali 10-20 part, Radix Codonopsis 15-25 part, Flos Carthami 5-15 part, Rhizoma Chuanxiong 10-20 part, Radix Angelicae Sinensis 10-15 part, Radix Rehmanniae Preparata 10-13 part, Semen Cucumidis sativi 8-15 part, Radix Notoginseng 5-15 part and Radix Glycyrrhizae 5-10 part.
A kind of compound medicine for the treatment of fracture of the present invention, source, the nature and flavor of this compound medicine component, return through and effect:
Flos Carthami: this product is the flower of feverfew Flos Carthami; Acrid in the mouth, hardship, warm in nature; Return liver, heart spleen channel; Promoting blood circulation to restore menstrual flow, promoting the circulation of QI, stasis-dispelling and pain-killing.
Rhizoma Chuanxiong: this product is the dry rhizome of samphire Rhizoma Chuanxiong; Acrid in the mouth, warm in nature; Return liver, gallbladder, pericardium channel; Blood-activating and qi-promoting, wind-expelling pain-stopping.
Radix Angelicae Sinensis: this product is the dry root of umbelliferae angelica; Sweet in the mouth, pungent, warm in nature; Enrich blood invigorate blood circulation, menstruction regulating and pain relieving.
Radix Rehmanniae Preparata: this product is the Preparation process product of Radix Rehmanniae; Sweet in the mouth, slightly warm in nature; Return liver, kidney channel; Nourishing YIN and supplementing blood, beneficial essence fill out marrow.
Semen Cucumidis sativi: this product is the seed of cucurbitaceous plant Fructus Cucumidis sativi; Sweet in the mouth, cool, nontoxic; Replenish the calcium, synthetism, bone strengthening, to keep fit.
Radix Notoginseng: this product is the rhizome of plant Radix Notoginseng; Sweet and slightly bitter taste, property is put down; Return liver, stomach, large intestine channel; Hemostasis, blood circulation promoting and blood stasis dispelling, subduing swelling and relieving pain, strengthening by means of tonics.
The Radix Astragali: this product is the root of the leguminous plant Radix Astragali; Sweet in the mouth, tepor; Return lung, spleen, liver, kidney channel; Invigorating QI to consolidate the body surface resistance, expelling pus and toxin by strengthening QI, diuresis, granulation promoting.
Radix Codonopsis: this product is the dry root of Campanulaceae Radix Codonopsis; Sweet in the mouth, property is put down; Return spleen, lung meridian; Invigorating the spleen and replenishing QI, spleen invigorating lung benefiting.
Radix Glycyrrhizae: this product is the dry root of glycyrrhizic legume, Glycyrrhiza inflata Bat. or Glycyrrhiza glabra L..Sweet in the mouth, property is put down; GUIXIN, lung, spleen, stomach warp; Invigorating the spleen and replenishing QI, heat-clearing and toxic substances removing, expelling phlegm for arresting cough, relieving spasm to stop pain, coordinating the actions of various ingredients in a prescription.
A kind of prescription analysis for the treatment of the compound medicine of fracture of the present invention:
A kind of compound medicine for the treatment of fracture of the present invention is with moringa oleifera leaf extractive, Radix Codonopsis for monarch drug, spirit keeping and blood activating, blood nourishing and calcium replenishing; With the Radix Astragali, Radix Angelicae Sinensis, Rhizoma Chuanxiong for ministerial drug, invigorating QI to consolidate the body surface resistance, promoting blood circulation to restore menstrual flow, stasis-dispelling and pain-killing, with Radix Notoginseng, Semen Cucumidis sativi, Radix Rehmanniae Preparata, Flos Carthami for adjuvant drug, removing pathogenic heat from blood and toxic substance from the body, replenishes the calcium and keeps fit, wind-expelling pain-stopping, detumescence and promoting granulation; With Radix Glycyrrhizae for making medicine, coordinating the actions of various ingredients in a prescription, makes all medicines arrange in pairs or groups, synergism, jointly reaches the effects such as blood nourishing and calcium replenishing, QI invigorating QI invigorating, promoting blood circulation to restore menstrual flow, promoting blood circulation to remove blood stasis, the body resistance strengthening and constitution consolidating and bone and muscle strengthening, has very significant effect to treatment fracture.
A kind of compound medicine for the treatment of fracture of the present invention compared with prior art, has following technical advantage:
1, compound medicine of the present invention have employed total flavones of Moringa oleifera leaf, by the synergism of total flavones of Moringa oleifera leaf and other Chinese medicinal components, better in the effect in strong bone of fostering the spirit of nobility and replenish the calcium of enriching blood;
2, compound medicine of the present invention is natural pure Chinese medicinal preparation, and toxic and side effects is little;
3, compound medicine of the present invention is in treatment fracture, there is the remarkable result of blood nourishing and calcium replenishing, QI invigorating QI invigorating, promoting blood circulation to restore menstrual flow, promoting blood circulation to remove blood stasis, the body resistance strengthening and constitution consolidating and bone and muscle strengthening, in addition, also have preparation efficacy stability, applied widely, be easy to advantages such as applying.
Detailed description of the invention
Embodiment 1 (to prepare 1000ml for radix, preparing decoction)
Preparation moringa oleifera leaf extractive:
A, take leaf of Moringa, dry 2.5h under temperature 50 C, pulverize, cross 45 mesh sieves, obtain leaf of Moringa powder;
B, get the leaf of Moringa powder that step a obtains, add the petroleum ether of leaf of Moringa opaque amount volume 3 times, reflux defat 3 times at temperature 68 DEG C, each 6h, crosses elimination filtrate, by filter residue and drying, obtain dry filtering residue;
The concentration adding filtering residue quality 12 times of volumes in c, the dry filtering residue that obtained by step b is 70% ethanol, 7min is processed under microwave power 600W, be placed in temperature 80 DEG C of tepidarium supersound extraction 2 times, each extraction 25min, filtration obtains extracting solution, merge extractive liquid, obtains total flavones of Moringa oleifera leaf crude extract;
D, the crude extract obtained in step c is joined in AB-8 type macroporous adsorptive resins, gradient elution is carried out successively with the ethanol that the distilled water of 2 times of column volumes and the volume fraction of 3 times of column volumes are respectively 30%, 50%, 70%, adjusted volume flow is 2mL/min, often 15min collected by pipe, with NaNO
2-Al (NO
3)
3-NaOH system color developing detection, under wavelength is 510nm, measure absorbance inspection know, collect the ethanol elution of 50% and 70%, merge eluent, eluent decompression recycling ethanol is concentrated into 1/5 of original volume, and obtain concentrated solution, concentrated solution being placed in temperature 60 C water-bath evaporate to dryness to relative density is the extractum of 1.25, dry under temperature 50 C, obtain moringa oleifera leaf extractive total flavones powder;
Prepare compound medicines:
E, take the Radix Astragali 6 parts, Radix Codonopsis 15 parts, 8 parts, Flos Carthami, Rhizoma Chuanxiong 5 parts, Radix Angelicae Sinensis 10 parts, 9 parts, Radix Rehmanniae Preparata, Semen Cucumidis sativi 5 parts, Radix Notoginseng 3 parts and 15 parts, Radix Glycyrrhizae, be placed on respectively in water and soak 20 minutes, decoct with water 2 times again, amount of water is 5 times that soak water consumption, each decoction 30 minutes, filter, obtain filtrate, merging filtrate, when being evaporated to temperature 80 DEG C, relative density is the extractum of 1.25-1.30, drying under reduced pressure, is ground into fine powder;
F, the moringa oleifera leaf extractive total flavones 5 parts steps d obtained mix with the fine powder that step e obtains, and stir, pharmaceutical methods makes decoction routinely.
Embodiment 2 (to prepare 1000ml for radix, preparing granule):
Preparation moringa oleifera leaf extractive:
A, take leaf of Moringa, dry 2.5h under temperature 50 C, pulverize, cross 45 mesh sieves, obtain leaf of Moringa powder;
B, get the leaf of Moringa powder that step a obtains, add the petroleum ether of leaf of Moringa opaque amount volume 3 times, reflux defat 3 times at temperature 68 DEG C, each 6h, crosses elimination filtrate, by filter residue and drying, obtain dry filtering residue;
The concentration adding filtering residue quality 12 times of volumes in c, the dry filtering residue that obtained by step b is 70% ethanol, 7min is processed under microwave power 600W, be placed in temperature 80 DEG C of tepidarium supersound extraction 2 times, each extraction 25min, filtration obtains extracting solution, merge extractive liquid, obtains total flavones of Moringa oleifera leaf crude extract;
D, the crude extract obtained in step c is joined in AB-8 type macroporous adsorptive resins, gradient elution is carried out successively with the ethanol that the distilled water of 2 times of column volumes and the volume fraction of 3 times of column volumes are respectively 30%, 50%, 70%, adjusted volume flow is 2mL/min, often 15min collected by pipe, with NaNO
2-Al (NO
3)
3-NaOH system color developing detection, under wavelength is 510nm, measure absorbance inspection know, collect the ethanol elution of 50% and 70%, merge eluent, eluent decompression recycling ethanol is concentrated into 1/5 of original volume, and obtain concentrated solution, concentrated solution being placed in temperature 60 C water-bath evaporate to dryness to relative density is the extractum of 1.25, dry under temperature 50 C, obtain moringa oleifera leaf extractive total flavones powder;
Prepare compound medicines:
E, take the Radix Astragali 30 parts, Radix Codonopsis 9 parts, 3 parts, Flos Carthami, Rhizoma Chuanxiong 10 parts, Radix Angelicae Sinensis 6 parts, 15 parts, Radix Rehmanniae Preparata, Semen Cucumidis sativi 3 parts, Radix Notoginseng 10 parts and 1 part, Radix Glycyrrhizae, be placed on respectively in water and soak 25 minutes, decoct with water 3 times again, amount of water is 6 times that soak water consumption, each decoction 40 minutes, filter, obtain filtrate, merging filtrate, when being evaporated to temperature 80 DEG C, relative density is the extractum of 1.25-1.30, drying under reduced pressure, is ground into fine powder;
F, 10 parts, moringa oleifera leaf extractive total flavones powder steps d obtained mix with the fine powder that step e obtains, and stir, pharmaceutical methods makes granule routinely.
Embodiment 3 (to prepare 1000ml for radix, preparing powder):
Preparation moringa oleifera leaf extractive:
A, take leaf of Moringa, dry 2.5h under temperature 50 C, pulverize, cross 45 mesh sieves, obtain leaf of Moringa powder;
B, get the leaf of Moringa powder that step a obtains, add the petroleum ether of leaf of Moringa opaque amount volume 3 times, reflux defat 3 times at temperature 68 DEG C, each 6h, crosses elimination filtrate, by filter residue and drying, obtain dry filtering residue;
The concentration adding filtering residue quality 12 times of volumes in c, the dry filtering residue that obtained by step b is 70% ethanol, 7min is processed under microwave power 600W, be placed in temperature 80 DEG C of tepidarium supersound extraction 2 times, each extraction 25min, filtration obtains extracting solution, merge extractive liquid, obtains total flavones of Moringa oleifera leaf crude extract;
D, the crude extract obtained in step c is joined in AB-8 type macroporous adsorptive resins, gradient elution is carried out successively with the ethanol that the distilled water of 2 times of column volumes and the volume fraction of 3 times of column volumes are respectively 30%, 50%, 70%, adjusted volume flow is 2mL/min, often 15min collected by pipe, with NaNO
2-Al (NO
3)
3-NaOH system color developing detection, under wavelength is 510nm, measure absorbance inspection know, collect the ethanol elution of 50% and 70%, merge eluent, eluent decompression recycling ethanol is concentrated into 1/5 of original volume, and obtain concentrated solution, concentrated solution being placed in temperature 60 C water-bath evaporate to dryness to relative density is the extractum of 1.25, dry under temperature 50 C, obtain moringa oleifera leaf extractive total flavones powder;
Prepare compound medicines:
E, take the Radix Astragali 20 parts, Radix Codonopsis 30 parts, 20 parts, Flos Carthami, Rhizoma Chuanxiong 18 parts, Radix Angelicae Sinensis 20 parts, 10 parts, Radix Rehmanniae Preparata, Semen Cucumidis sativi 15 parts, Radix Notoginseng 20 parts and 10 parts, Radix Glycyrrhizae, be placed on respectively in water and soak 30 minutes, decoct with water 3 times again, amount of water is 8 times that soak water consumption, each decoction 60 minutes, filter, obtain filtrate, merging filtrate, when being evaporated to temperature 80 DEG C, relative density is the extractum of 1.25-1.30, drying under reduced pressure, is ground into fine powder;
F, 20 parts, moringa oleifera leaf extractive total flavones powder steps d obtained mix with the fine powder that step e obtains, and stir, pharmaceutical methods makes powder routinely.
Embodiment 4 (to prepare 1000ml for radix, preparing tablet):
Preparation moringa oleifera leaf extractive:
A, take leaf of Moringa, dry 2.5h under temperature 50 C, pulverize, cross 45 mesh sieves, obtain leaf of Moringa powder;
B, get the leaf of Moringa powder that step a obtains, add the petroleum ether of leaf of Moringa opaque amount volume 3 times, reflux defat 3 times at temperature 68 DEG C, each 6h, crosses elimination filtrate, by filter residue and drying, obtain dry filtering residue;
The concentration adding filtering residue quality 12 times of volumes in c, the dry filtering residue that obtained by step b is 70% ethanol, 7min is processed under microwave power 600W, be placed in temperature 80 DEG C of tepidarium supersound extraction 2 times, each extraction 25min, filtration obtains extracting solution, merge extractive liquid, obtains total flavones of Moringa oleifera leaf crude extract;
D, the crude extract obtained in step c is joined in AB-8 type macroporous adsorptive resins, gradient elution is carried out successively with the ethanol that the distilled water of 2 times of column volumes and the volume fraction of 3 times of column volumes are respectively 30%, 50%, 70%, adjusted volume flow is 2mL/min, often 15min collected by pipe, with NaNO
2-Al (NO
3)
3-NaOH system color developing detection, under wavelength is 510nm, measure absorbance inspection know, collect the ethanol elution of 50% and 70%, merge eluent, eluent decompression recycling ethanol is concentrated into 1/5 of original volume, and obtain concentrated solution, concentrated solution being placed in temperature 60 C water-bath evaporate to dryness to relative density is the extractum of 1.25, dry under temperature 50 C, obtain moringa oleifera leaf extractive total flavones powder;
Prepare compound medicines:
E, take the Radix Astragali 10 parts, Radix Codonopsis 18 parts, 11 parts, Flos Carthami, Rhizoma Chuanxiong 25 parts, Radix Angelicae Sinensis 15 parts, 12 parts, Radix Rehmanniae Preparata, Semen Cucumidis sativi 10 parts, Radix Notoginseng 15 parts and 5 parts, Radix Glycyrrhizae, be placed on respectively in water and soak 25 minutes, decoct with water 2 times again, amount of water is 7 times that soak water consumption, each decoction 90 minutes, filter, obtain filtrate, merging filtrate, when being evaporated to temperature 80 DEG C, relative density is the extractum of 1.25-1.30, drying under reduced pressure, is ground into fine powder;
F, the moringa oleifera leaf extractive total flavones 25 parts steps d obtained mix with the fine powder that step e obtains, and stir, pharmaceutical methods makes tablet routinely.
Embodiment 5 (to prepare 1000ml for radix, preparing capsule):
Preparation moringa oleifera leaf extractive:
A, take leaf of Moringa, dry 2.5h under temperature 50 C, pulverize, cross 45 mesh sieves, obtain leaf of Moringa powder;
B, get the leaf of Moringa powder that step a obtains, add the petroleum ether of leaf of Moringa opaque amount volume 3 times, reflux defat 3 times at temperature 68 DEG C, each 6h, crosses elimination filtrate, by filter residue and drying, obtain dry filtering residue;
The concentration adding filtering residue quality 12 times of volumes in c, the dry filtering residue that obtained by step b is 70% ethanol, 7min is processed under microwave power 600W, be placed in temperature 80 DEG C of tepidarium supersound extraction 2 times, each extraction 25min, filtration obtains extracting solution, merge extractive liquid, obtains total flavones of Moringa oleifera leaf crude extract;
D, the crude extract obtained in step c is joined in AB-8 type macroporous adsorptive resins, gradient elution is carried out successively with the ethanol that the distilled water of 2 times of column volumes and the volume fraction of 3 times of column volumes are respectively 30%, 50%, 70%, adjusted volume flow is 2mL/min, often 15min collected by pipe, with NaNO
2-Al (NO
3)
3-NaOH system color developing detection, under wavelength is 510nm, measure absorbance inspection know, collect the ethanol elution of 50% and 70%, merge eluent, eluent decompression recycling ethanol is concentrated into 1/5 of original volume, and obtain concentrated solution, concentrated solution being placed in temperature 60 C water-bath evaporate to dryness to relative density is the extractum of 1.25, dry under temperature 50 C, obtain moringa oleifera leaf extractive total flavones powder;
Prepare compound medicines:
E, take the Radix Astragali 23 parts, Radix Codonopsis 22 parts, 15 parts, Flos Carthami, Rhizoma Chuanxiong 19 parts, Radix Angelicae Sinensis 14 parts, 11 parts, Radix Rehmanniae Preparata, Semen Cucumidis sativi 17 parts, Radix Notoginseng 14 parts and 9 parts, Radix Glycyrrhizae, be placed on respectively in water and soak 28 minutes, decoct with water 2 times again, amount of water is 6 times that soak water consumption, each decoction 50 minutes, filter, obtain filtrate, merging filtrate, when being evaporated to temperature 80 DEG C, relative density is the extractum of 1.25-1.30, drying under reduced pressure, is ground into fine powder;
F, the moringa oleifera leaf extractive total flavones 30 parts steps d obtained mix with the fine powder that step e obtains, and stir, pharmaceutical methods makes capsule routinely.
Embodiment 6 (to prepare 1000ml for radix, preparing pill)
Preparation moringa oleifera leaf extractive:
A, take leaf of Moringa, dry 2.5h under temperature 50 C, pulverize, cross 45 mesh sieves, obtain leaf of Moringa powder;
B, get the leaf of Moringa powder that step a obtains, add the petroleum ether of leaf of Moringa opaque amount volume 3 times, reflux defat 3 times at temperature 68 DEG C, each 6h, crosses elimination filtrate, by filter residue and drying, obtain dry filtering residue;
The concentration adding filtering residue quality 12 times of volumes in c, the dry filtering residue that obtained by step b is 70% ethanol, 7min is processed under microwave power 600W, be placed in temperature 80 DEG C of tepidarium supersound extraction 2 times, each extraction 25min, filtration obtains extracting solution, merge extractive liquid, obtains total flavones of Moringa oleifera leaf crude extract;
D, the crude extract obtained in step c is joined in AB-8 type macroporous adsorptive resins, gradient elution is carried out successively with the ethanol that the distilled water of 2 times of column volumes and the volume fraction of 3 times of column volumes are respectively 30%, 50%, 70%, adjusted volume flow is 2mL/min, often 15min collected by pipe, with NaNO
2-Al (NO
3)
3-NaOH system color developing detection, under wavelength is 510nm, measure absorbance inspection know, collect the ethanol elution of 50% and 70%, merge eluent, eluent decompression recycling ethanol is concentrated into 1/5 of original volume, and obtain concentrated solution, concentrated solution being placed in temperature 60 C water-bath evaporate to dryness to relative density is the extractum of 1.25, dry under temperature 50 C, obtain moringa oleifera leaf extractive total flavones powder;
Prepare compound medicines:
E, take the Radix Astragali 15 parts, Radix Codonopsis 25 parts, 5 parts, Flos Carthami, Rhizoma Chuanxiong 20 parts, Radix Angelicae Sinensis 8 parts, 13 parts, Radix Rehmanniae Preparata, Semen Cucumidis sativi 8 parts, Radix Notoginseng 5 parts and 5 parts, Radix Glycyrrhizae, be placed on respectively in water and soak 30 minutes, decoct with water 3 times again, amount of water is 5 times that soak water consumption, each decoction 90 minutes, filter, obtain filtrate, merging filtrate, when being evaporated to temperature 80 DEG C, relative density is the extractum of 1.25-1.30, drying under reduced pressure, is ground into fine powder;
F, 30 parts, moringa oleifera leaf extractive total flavones powder steps d obtained mix with the fine powder that step e obtains, and stir, pharmaceutical methods makes pill routinely.
Embodiment 7 (to prepare 1000ml for radix, preparing tablet)
Preparation moringa oleifera leaf extractive:
A, take leaf of Moringa, dry 2.5h under temperature 50 C, pulverize, cross 45 mesh sieves, obtain leaf of Moringa powder;
B, get the leaf of Moringa powder that step a obtains, add the petroleum ether of leaf of Moringa opaque amount volume 3 times, reflux defat 3 times at temperature 68 DEG C, each 6h, crosses elimination filtrate, by filter residue and drying, obtain dry filtering residue;
The concentration adding filtering residue quality 12 times of volumes in c, the dry filtering residue that obtained by step b is 70% ethanol, 7min is processed under microwave power 600W, be placed in temperature 80 DEG C of tepidarium supersound extraction 2 times, each extraction 25min, filtration obtains extracting solution, merge extractive liquid, obtains total flavones of Moringa oleifera leaf crude extract;
D, the crude extract obtained in step c is joined in AB-8 type macroporous adsorptive resins, gradient elution is carried out successively with the ethanol that the distilled water of 2 times of column volumes and the volume fraction of 3 times of column volumes are respectively 30%, 50%, 70%, adjusted volume flow is 2mL/min, often 15min collected by pipe, with NaNO
2-Al (NO
3)
3-NaOH system color developing detection, under wavelength is 510nm, measure absorbance inspection know, collect the ethanol elution of 50% and 70%, merge eluent, eluent decompression recycling ethanol is concentrated into 1/5 of original volume, and obtain concentrated solution, concentrated solution being placed in temperature 60 C water-bath evaporate to dryness to relative density is the extractum of 1.25, dry under temperature 50 C, obtain moringa oleifera leaf extractive total flavones powder;
Prepare compound medicines:
E, take the Radix Astragali 28 parts, Radix Codonopsis 10 parts, 12 parts, Flos Carthami, Rhizoma Chuanxiong 30 parts, Radix Angelicae Sinensis 12 parts, 9 parts, Radix Rehmanniae Preparata, Semen Cucumidis sativi 8 parts, Radix Notoginseng 8 parts and 12 parts, Radix Glycyrrhizae, be placed on respectively in water and soak 20 minutes, decoct with water 2 times again, amount of water is 8 times that soak water consumption, each decoction 80 minutes, filter, obtain filtrate, merging filtrate, when being evaporated to temperature 80 DEG C, relative density is the extractum of 1.25-1.30, drying under reduced pressure, is ground into fine powder;
F, 8 parts, moringa oleifera leaf extractive total flavones powder steps d obtained mix with the fine powder that step e obtains, and stir, pharmaceutical methods makes tablet routinely.
Embodiment 8 (to prepare 1000ml for radix, preparing tablet)
Preparation moringa oleifera leaf extractive:
A, take leaf of Moringa, dry 2.5h under temperature 50 C, pulverize, cross 45 mesh sieves, obtain leaf of Moringa powder;
B, get the leaf of Moringa powder that step a obtains, add the petroleum ether of leaf of Moringa opaque amount volume 3 times, reflux defat 3 times at temperature 68 DEG C, each 6h, crosses elimination filtrate, by filter residue and drying, obtain dry filtering residue;
The concentration adding filtering residue quality 12 times of volumes in c, the dry filtering residue that obtained by step b is 70% ethanol, 7min is processed under microwave power 600W, be placed in temperature 80 DEG C of tepidarium supersound extraction 2 times, each extraction 25min, filtration obtains extracting solution, merge extractive liquid, obtains total flavones of Moringa oleifera leaf crude extract;
D, the crude extract obtained in step c is joined in AB-8 type macroporous adsorptive resins, gradient elution is carried out successively with the ethanol that the distilled water of 2 times of column volumes and the volume fraction of 3 times of column volumes are respectively 30%, 50%, 70%, adjusted volume flow is 2mL/min, often 15min collected by pipe, with NaNO
2-Al (NO
3)
3-NaOH system color developing detection, under wavelength is 510nm, measure absorbance inspection know, collect the ethanol elution of 50% and 70%, merge eluent, eluent decompression recycling ethanol is concentrated into 1/5 of original volume, and obtain concentrated solution, concentrated solution being placed in temperature 60 C water-bath evaporate to dryness to relative density is the extractum of 1.25, dry under temperature 50 C, obtain moringa oleifera leaf extractive total flavones powder;
Prepare compound medicines:
E, take the Radix Astragali 18 parts, Radix Codonopsis 12 parts, 18 parts, Flos Carthami, Rhizoma Chuanxiong 8 parts, Radix Angelicae Sinensis 18 parts, 15 parts, Radix Rehmanniae Preparata, Semen Cucumidis sativi 20 parts, Radix Notoginseng 18 parts and 3 parts, Radix Glycyrrhizae, be placed on respectively in water and soak 22 minutes, decoct with water 3 times again, amount of water is 6 times that soak water consumption, each decoction 45 minutes, filter, obtain filtrate, merging filtrate, when being evaporated to temperature 80 DEG C, relative density is the extractum of 1.25-1.30, drying under reduced pressure, is ground into fine powder;
F, 25 parts, moringa oleifera leaf extractive total flavones powder steps d obtained mix with the fine powder that step e obtains, and stir, pharmaceutical methods makes tablet routinely.
Embodiment 9 (to prepare 1000ml for radix, preparing capsule)
Preparation moringa oleifera leaf extractive:
A, take leaf of Moringa, dry 2.5h under temperature 50 C, pulverize, cross 45 mesh sieves, obtain leaf of Moringa powder;
B, get the leaf of Moringa powder that step a obtains, add the petroleum ether of leaf of Moringa opaque amount volume 3 times, reflux defat 3 times at temperature 68 DEG C, each 6h, crosses elimination filtrate, by filter residue and drying, obtain dry filtering residue;
The concentration adding filtering residue quality 12 times of volumes in c, the dry filtering residue that obtained by step b is 70% ethanol, 7min is processed under microwave power 600W, be placed in temperature 80 DEG C of tepidarium supersound extraction 2 times, each extraction 25min, filtration obtains extracting solution, merge extractive liquid, obtains total flavones of Moringa oleifera leaf crude extract;
D, the crude extract obtained in step c is joined in AB-8 type macroporous adsorptive resins, gradient elution is carried out successively with the ethanol that the distilled water of 2 times of column volumes and the volume fraction of 3 times of column volumes are respectively 30%, 50%, 70%, adjusted volume flow is 2mL/min, often 15min collected by pipe, with NaNO
2-Al (NO
3)
3-NaOH system color developing detection, under wavelength is 510nm, measure absorbance inspection know, collect the ethanol elution of 50% and 70%, merge eluent, eluent decompression recycling ethanol is concentrated into 1/5 of original volume, and obtain concentrated solution, concentrated solution being placed in temperature 60 C water-bath evaporate to dryness to relative density is the extractum of 1.25, dry under temperature 50 C, obtain moringa oleifera leaf extractive total flavones powder;
Prepare compound medicines:
E, take the Radix Astragali 8 parts, Radix Codonopsis 30 parts, 20 parts, Flos Carthami, Rhizoma Chuanxiong 30 parts, Radix Angelicae Sinensis 15 parts, 10 parts, Radix Rehmanniae Preparata, Semen Cucumidis sativi 12 parts, Radix Notoginseng 3 parts and 8 parts, Radix Glycyrrhizae, be placed on respectively in water and soak 30 minutes, decoct with water 3 times again, amount of water is 8 times that soak water consumption, each decoction 70 minutes, filter, obtain filtrate, merging filtrate, when being evaporated to temperature 80 DEG C, relative density is the extractum of 1.25-1.30, drying under reduced pressure, is ground into fine powder;
F, 8 parts, moringa oleifera leaf extractive total flavones powder steps d obtained mix with the fine powder that step e obtains, and stir, pharmaceutical methods makes capsule routinely.
Embodiment 10
Pharmaceutical composition pharmacodynamic study of the present invention
Compound medicine of the present invention is to the clinical observation on the therapeutic effect of fracture clinical volunteers patient:
Diagnostic criteria: determine that the diagnostic criteria of this test is as follows with reference to " new Chinese medicine guideline of clinical investigations " 2002 " guideline of clinical investigations of new Chinese medicine treatment traumatic fracture ":
Western medicine diagnose standard:
Traumatic fracture diagnostic criteria:
There is the trauma history of direct or indirect violence;
General Symptoms: uncomplicated simple fracture, General Symptoms is not obvious or not serious; Shock can be there is or merge visceral organ injury in serious wound, combined injury; Open fracture can concurrent infection or and go crazy, blood vessel injury, there is corresponding General Symptoms;
Local symptom: pain pain and tenderness, swelling, dysfunction, deformity, abnormal movement, bony crepitus;
X-ray checks: the diagnosis that can clearly fracture, can confirm the localized variation such as classification of fracture, moving direction, fracture end form, and get rid of pathologisch Bruch.
Tcm diagnosis standard:
Syndrome of qi stagnation and blood stasis: primary symptom: fracture, pain, swelling, ecchymosis; Secondary disease: thirsty, dark coloured urine, constipation, red tongue or have ecchymosis, yellow fur, floating and rapid pulse or stringy and tense pulse;
The sluggish card of congestion: primary symptom: fracture does not connect or bone connects hard, and alleviation of pain, swells and disappear to the greatest extent; Secondary disease: dimly red tongue, yellow and thin fur, stringy pulse;
Deficiency of qi and blood is demonstrate,proved: primary symptom: bone connects not hard, dizziness and blurred vision, pale white complexion; Secondary disease: deficiency of QI with disinclination to talk, spiritlessness and weakness, light red tongue, thready pulse;
Caused by liver and kidney deficiency is demonstrate,proved: primary symptom: bone connects not hard, soreness of waist and knee joint, and limbs wither soft; Secondary disease: spiritlessness and weakness, light red tongue, thin fur, thready pulse.
Exclusion standard: (1) pathologisch Bruch caused by osteopathia; (2) open fracture has soft tissue defects or the infected; (3) suckling gestation or preparing gestation women; (4) allergic constitution or to multi-medicament allergy sufferers; (5) severe primary disease and the psychotics such as the heart, brain, liver, kidney, hemopoietic system, hormonal system is merged; (6) state of an illness is critical, is difficult to make definite estimator to the effectiveness of new drug and safety.
Clinical trial
1, physical data: all selected fracture clinical volunteers patients are divided into two groups at random, treatment group 110 example, wherein male patient 62 example, female patient 48 example; Matched group 110 example, wherein male patient 68 example, female patient 42 example.Age is 20 years old-79 years old, 49.5 years old mean age, two groups of influence factors such as age, symptom, and through statistical procedures, there was no significant difference, meets a point set condition.
2, test method:
Treatment group: take tablet (0.3g*100 sheet/bottle) prepared by the present invention, every day 3 times, each 3, serve on 3 months;
Matched group: take bone setting tablet (Pan Shoude, accurate word Z21021461, the 0.33g*36 sheet/box of traditional Chinese medicines), every day 3 times, each 4, serve on 3 months.
3, curative effect determinate standard:
Effective: pain is eased substantially, fracture site is red, swollen, heat all obviously alleviates,
Effective: pain obtains larger alleviation, and fracture site is red, swollen, heat all alleviates;
Invalid: pain does not have alleviation, fracture site is red, swollen, heat does not all obtain and obviously alleviates
4, therapeutic outcome, as shown in table 1;
Table 1 therapeutic effect
| Group | Number of cases | Effective (rate) | Effectively | Invalid | Total effective rate |
| Treatment group | 110 | 90(81.8％) | 14 | 6 | 94.5％ |
| Matched group | 110 | 65(59.1％) | 18 | 27 | 75.4％ |
5, conclusion:
This test adopts bone setting tablet as a control group, bone setting tablet has blood circulation promoting and blood stasis dispelling, reducing swelling and alleviating pain, effect of relaxing muscles and tendons bone strengthening, for traumatic injury, lumbar sprain and QI divergeny, injured in the sinews or bones, swelling and pain due to blood stasis, damage is red and swollen waits disease, by with the comparing of compound medicine therapeutic effect of the present invention, highlight the effect of compound medicine of the present invention in treatment fracture, its result shows: compound medicine of the present invention reaches more than 80% for the obvious effective rate for the treatment of fracture, total effective rate reaches more than 90%, far above obvious effective rate and the total effective rate of matched group, visible, compound medicine of the present invention is evident in efficacy, total effective rate is high, can be used as the drug use for the treatment of fracture.
Embodiment 11
Pharmaceutical composition of the present invention is on the impact of White Rabbit fracture model bone density:
Test material
Experimental animal: White Rabbit, male and female half and half, 1.5-2.5kg, are provided by Zhongshan University center; Get White Rabbit 60, be divided into 5 groups at random, often organize 12, be respectively model group (equal-volume normal saline), Radix Lamiophlomidis Rotatae group (1.0g/kg ﹒ d), pharmaceutical composition low dose group of the present invention (0.27g/kg ﹒ d), middle dosage group (0.81g/kg ﹒ d) and high dose group (1.62g/kg ﹒ d);
Instrument: Hitachi 7020 automatic clinical chemistry analyzer, Japanese Hitachi company; Absorptiometry (Piximusbonedensito-meter, LUNAR company of the U.S.);
Trial drug: pharmaceutical composition prepared by the embodiment of the present invention 1; Radix Lamiophlomidis Rotatae capsule, Gansu Duyiwei Biological Pharmaceutical Co., Ltd, 0.3g*50 grain/bottle;
Test method:
After White Rabbit adaptability raises one week, with 3% Nembutal sodium solution according to after the anesthesia of 1ml/kg dose intravenous, both sides forelimb cropping, 75% alcohol disinfecting, is aseptically widely about 3mm with horizontal being drilled to of medical operation dental burr epimere 1/3 intersection in the radius of both sides, go deep into medullary cavity and form bone injury, skin suture, Post operation starts gastric infusion, successive administration next day, within after administration one week, to get 7 in each group of rabbit to surrounding, measure indices; And intercepting both sides radius respectively at second week after administration, 4th week, its length comprises damage and each normal reach sclerotin up and down, measures bone density, observe union of fracture situation with U.S. LUNAR company Piximusbonedensito-meter analyzer.
Result of the test:
Table 2 pharmaceutical composition of the present invention is on the impact of White Rabbit fracture model bone density
▲compared with model group, p<0.05;
▲ ▲compared with model group, p<0.01.
Conclusion:
As shown in Table 2, successive administration is after two weeks, and the bone density of Radix Lamiophlomidis Rotatae group and compound medicine low dose group of the present invention, middle dosage group and high dose group increases all to some extent, and has significant difference (p<0.05) compared with model group; After successive administration surrounding, the amplitude that the bone density of Radix Lamiophlomidis Rotatae group and compound medicine low dose group of the present invention, middle dosage group and high dose group increases is larger, much larger than the bone density after two weeks, and there is pole significant difference (p<0.01) compared with model group; As can be seen here, compound medicine of the present invention has accelerates bone ore deposit sedimentation rate, improves bone density, promotes the function of fracture site healing.
Claims (2)
1. treat the compound medicine of fracture for one kind, it is characterized in that making decoction, granule, powder, capsule, pill or tablet by crude drug moringa oleifera leaf extractive 5-30 part, Radix Astragali 6-30 part, Radix Codonopsis 9-30 part, Flos Carthami 3-20 part, Rhizoma Chuanxiong 5-30 part, Radix Angelicae Sinensis 6-20 part, Radix Rehmanniae Preparata 9-15 part, Semen Cucumidis sativi 3-20 part, Radix Notoginseng 3-20 part and Radix Glycyrrhizae 1-15 part, concrete operations follow these steps to carry out:
Preparation moringa oleifera leaf extractive:
A, take leaf of Moringa, dry 2.5h under temperature 50 C, pulverize, cross 45 mesh sieves, obtain leaf of Moringa powder;
B, get the leaf of Moringa powder that step a obtains, add the petroleum ether of leaf of Moringa opaque amount volume 3 times, reflux defat 3 times at temperature 68 DEG C, each 6h, crosses elimination filtrate, by filter residue and drying, obtain dry filtering residue;
The concentration adding filtering residue quality 12 times of volumes in c, the dry filtering residue that obtained by step b is 70% ethanol, 7min is processed under microwave power 600W, be placed in temperature 80 DEG C of tepidarium supersound extraction 2 times, each extraction 25min, filtration obtains extracting solution, merge extractive liquid, obtains total flavones of Moringa oleifera leaf crude extract;
D, the crude extract obtained in step c is joined in AB-8 type macroporous adsorptive resins, gradient elution is carried out successively with the ethanol that the distilled water of 2 times of column volumes and the volume fraction of 3 times of column volumes are respectively 30%, 50%, 70%, adjusted volume flow is 2mL/min, and often 15min collected by pipe, with NaNO
2-Al (NO
3)
3-NaOH system color developing detection, under wavelength is 510nm, measure absorbance inspection know, collect the ethanol elution of 50% and 70%, merge eluent, eluent decompression recycling ethanol is concentrated into 1/5 of original volume, and obtain concentrated solution, concentrated solution being placed in temperature 60 C water-bath evaporate to dryness to relative density is the extractum of 1.25, dry at 50 DEG C, obtain moringa oleifera leaf extractive total flavones powder;
Prepare compound medicines:
E, take the Radix Astragali, Radix Codonopsis, Flos Carthami, Rhizoma Chuanxiong, Radix Angelicae Sinensis, Radix Rehmanniae Preparata, Semen Cucumidis sativi, Radix Notoginseng and Radix Glycyrrhizae, be placed in water respectively and soak 20-30 minute, decoct with water 2-3 time, amount of water is 5-8 times that soaks water consumption again, each decoction 30-90 minute, filter, obtain filtrate, merging filtrate, when being evaporated to temperature 80 DEG C, relative density is the extractum of 1.25-1.30, drying under reduced pressure, is ground into fine powder;
F, moringa oleifera leaf extractive total flavones powder steps d obtained mix with the fine powder that step e obtains, and stir, and must treat the compound medicine of fracture.
2. the compound medicine for the treatment of fracture as claimed in claim 1, is characterized in that making decoction, granule, powder, capsule, pill or tablet by crude drug moringa oleifera leaf extractive 10-20 part, Radix Astragali 10-20 part, Radix Codonopsis 15-25 part, Flos Carthami 5-15 part, Rhizoma Chuanxiong 10-20 part, Radix Angelicae Sinensis 10-15 part, Radix Rehmanniae Preparata 10-13 part, Semen Cucumidis sativi 8-15 part, Radix Notoginseng 5-15 part and Radix Glycyrrhizae 5-10 part.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510580362.7A CN105168537A (en) | 2015-09-14 | 2015-09-14 | Compound medicine for treating bone fracture and preparation method of compound medicine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510580362.7A CN105168537A (en) | 2015-09-14 | 2015-09-14 | Compound medicine for treating bone fracture and preparation method of compound medicine |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105168537A true CN105168537A (en) | 2015-12-23 |
Family
ID=54891292
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510580362.7A Pending CN105168537A (en) | 2015-09-14 | 2015-09-14 | Compound medicine for treating bone fracture and preparation method of compound medicine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105168537A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3903798A1 (en) * | 2020-04-27 | 2021-11-03 | Lulin Wang | Bone-strengthening pill (bsp) as a dietary supplement to improve blood circulation and strengthen bone and muscle |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103494999A (en) * | 2013-08-28 | 2014-01-08 | 李文平 | Pharmaceutical composition and pharmaceutical preparation, preparation method thereof and application thereof |
| CN104971140A (en) * | 2015-07-24 | 2015-10-14 | 青岛蓝盛洋医药生物科技有限责任公司 | Medicine composition for treating bone fracture and preparation method thereof |
-
2015
- 2015-09-14 CN CN201510580362.7A patent/CN105168537A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103494999A (en) * | 2013-08-28 | 2014-01-08 | 李文平 | Pharmaceutical composition and pharmaceutical preparation, preparation method thereof and application thereof |
| CN104971140A (en) * | 2015-07-24 | 2015-10-14 | 青岛蓝盛洋医药生物科技有限责任公司 | Medicine composition for treating bone fracture and preparation method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3903798A1 (en) * | 2020-04-27 | 2021-11-03 | Lulin Wang | Bone-strengthening pill (bsp) as a dietary supplement to improve blood circulation and strengthen bone and muscle |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104971140A (en) | Medicine composition for treating bone fracture and preparation method thereof | |
| CN104887747A (en) | Traditional Chinese medicine composition for treating cerebral thrombosis and preparation method therefor | |
| CN104815318A (en) | Traditional Chinese medicine preparation for treating gout | |
| CN104623376B (en) | Be used for the treatment of prosopalgic medicine | |
| CN103007037A (en) | Traditional Chinese medicine preparation for treating irregular menstruation | |
| CN105194338A (en) | Traditional Chinese medicine composition for treating uterus cold and preparation method thereof | |
| CN105214010A (en) | A kind of Chinese medicine preparation for the treatment of chronic cholecystitis | |
| CN104548049A (en) | Traditional Chinese medicine composition for treating senile osteoporosis and preparation method thereof | |
| CN105168537A (en) | Compound medicine for treating bone fracture and preparation method of compound medicine | |
| CN105362647A (en) | Method for preparing pharmaceutical composition for treating gynecological hemorrhage syndrome | |
| CN104887950A (en) | Mastitis treatment traditional Chinese medicine compound | |
| CN105381307A (en) | Traditional Chinese medicine composition for treating mastitis and preparing method thereof | |
| CN105169265A (en) | Traditional Chinese medicine composition for treating hemiplegia and preparation method of traditional Chinese medicine composition | |
| CN104800610A (en) | Traditional Chinese medicine preparation for treating Parkinson's disease and preparation method thereof | |
| CN104547862A (en) | Use of traditional Chinese medicine preparation in preparation of medicine for treating bone fracture | |
| CN105267292A (en) | Pharmaceutical composition for treating bone fracture and preparation method of pharmaceutical composition for treating bone fracture | |
| CN105213481A (en) | A kind of pharmaceutical composition for the treatment of fracture and preparation method thereof | |
| CN104873854A (en) | Traditional Chinese medicine preparation for rteriolar nephrosclerosis and preparation method thereof | |
| CN105311183A (en) | Pharmaceutical composition containing folium artemisiae argyi for treating fracture and preparation method of pharmaceutical composition | |
| CN105327299A (en) | Medicine for treating idiopathic thrombocytopenic purpura | |
| CN105250634A (en) | Traditional Chinese medicine composition for treating postpartum constipation and application thereof | |
| CN105012637A (en) | Medicine for treating tumors of breasts | |
| CN104524331B (en) | Prevent the drug of mumps | |
| CN104645233A (en) | Traditional Chinese medicine preparation for treating hyperplasia of mammary glands | |
| CN104491312A (en) | Use of tradtional Chinese medicine preparation in preparing medicines for treating prostatitis and prostatauxe |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20151223 |
|
| RJ01 | Rejection of invention patent application after publication |