CN105168257A - Processing method of pearl liquid, pearl ginseng liquid and pearl powder easily absorbed by human body - Google Patents
Processing method of pearl liquid, pearl ginseng liquid and pearl powder easily absorbed by human body Download PDFInfo
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Abstract
The invention relates to a method of producing a pearl liquid, a pearl ginseng liquid and pearl powder easily absorbed by a human body by using pearl powder as a traditional Chinese medicinal material, which belongs to the technical field of production of the pearl liquid. The method comprises the following steps of firstly, preparing the pearl powder or pearl layer powder; secondly, dissolving with hydrochloric acid until the PH value reaches 6.4 to 6.6; thirdly, diluting, and filtering with filter paper to obtain conchiolin; fourthly, combining a plurality of parts of the conchiolin in the third step, and hydrolyzing the combined conchiolin with citric acid; fifthly, neutralizing until the PH value reaches 4.4 to 4.6 to obtain liquid A; sixthly, continuously neutralizing the liquid A until the PH value reaches 8, and precipitating the liquid for 3 to 4 hours; seventhly, filtering with filter paper to obtain liquid B and filter residues C; eighthly, adding the liquid B into a blending formula for conditioning according to the weight percentage of the preset volumetric yield of products; ninthly, filtering with filter paper to discharge the residues; tenthly, filling with the liquid and sterilizing to obtain a pearl liquid or pearl liquid ginseng liquid product; eleventhly, drying, crushing and packaging the filter residues C to obtain a calcium citrate supplementing agent; twelfthly, drying, crushing and packaging the liquid A.
Description
Technical field
The present invention relates to and utilize Chinese crude drug Margarita powder and Radix Ginseng, produce the method that Margarita liquid, Margarita ginseng liquid and human body easily absorb Margarita powder, belong to Margarita production technical field.
Background technology
Margarita is exactly a kind of rare Chinese medicine since ancient times, has to calm the nerves relieving convulsion, keeps fit strong soul effect, can heat-clearing and toxic substances removing, removing nebula improving eyesight.For treatment fever of children infantile convulsion, the diseases such as metrorrhagia, all have good therapeutic effect, are the good medicine of traditional Chinese medical science Ge Ke outbalance.Modern ESR technology has proved that Margarita has the ability removing ultra-oxygen anion free radical and hydroxyl radical free radical, really has the effect of slow down aging.Margarita is also skin care good product.
Radix Ginseng function: there is strongly invigorating primordial QI, invigorating the spleen to benefit the lung, Fructus Alpiniae Oxyphyllae of calming the nerves, antitumor and the anti-ageing effect of waiting for a long time.
Both Margarita and Radix Ginseng are combined, bring out the best in each other!
Why does Margarita have so magical effect? according to modern science analysis; Margarita is not only containing 18 kinds of gal4 amino acids; also containing more taurine and ornithine; they are very important to human body; it is the required material that neonatal cerebral is grown; and there is protection cardiac muscle, the effects such as anti-arrhythmia.In addition, Margarita is also containing a large amount of calcium, and it is one of most important element of human body, is also the abundantest cation.Tens gunnysack polishing Pteria martensii Dunker shells were once placed on moist one-storey house by us, and after 3 years, gunnysack rots entirely, and pearl shell mildew does not have yet.To change after gunnysack 3 years, gunnysack rots again entirely, and pearl shell still a mildew do not have yet.This illustrates that Margarita and Margarita (freshwater mussel) shell are very magical materials.
The use problem of Margarita layer powder.The textbook " Zhang Xi, Qi Zhongyan work--malacology outline " of China authority thinks that shell divides three layers, and nexine nacreous layer is the aragonite structure of calcium carbonate; Middle level prismatic layer and top layer horny layer are all the conclusions of calcium carbonate calcite structure, and this conclusion may from abroad copying, and they do not do the experiment of this respect.The present conchiferous nacreous layer of Margarita layer powder bag and prismatic layer, by above theoretical, prismatic layer is not the aragonite structure of Margarita, cannot as the purposes of Margarita medicine.
Can according to our research: 1. Chen Jun person of outstanding talent, Chen Guiqing, Zhang Xiujun.Pinctadamaxima (Jameson) shell, pearl oyster shell, the crystal structure of black pearl oyster shell and pinctada fucata shell.Marine drug 1985-1.2. Chen Jun person of outstanding talent, Chen Guiqing.Pteria penguin shell, Pinctada chemnitzi shell, the crystal structure of purple river precious jade shell and Perna uiridis (Linnaeus) shell.Marine drug 1986-1.3. Chen Gui minister in ancient times, Chen Junhao, oldly wafts.The natural peral of Pteria martensii Dunker and the research of shell thereof.Marine drug 1986-3-4.Within 1987, obtain " marine drug " " best paper prize ".Prove that the prismatic layer of pearl shell is also the aragonite structure of Margarita, can as the purposes of Margarita medicine.
Though Margarita contains the material of so multiple beneficial human body, the calcium contained by it exists as calcium carbonate, can only be slightly soluble in water (just dissolving 1.39g in 100,000g saturation water), not easily absorbed by the body.Contained aminoacid exists with the form of angle glutelin, it is that (molecular weight is 5 for macromole, more than 000), moreover not easily absorbed by the body. the organic matters such as these gal4 amino acids, nonprotein amino acid and biological active substances, almost surround by the calcium carbonate of 24 times of indissolubles, so though Margarita is good, human body also can only decompose absorption a bit by gastric acid, will better absorb it and have any problem.For this reason, need to solve two problems.(1), by the dissolution of calcium carbonate of Margarita, discharge the angle glutelin of Margarita.(2) the macromole angle glutelin of Margarita is hydrolyzed into micromolecule aminoacid, so that human body directly absorbs.
The object of hydrolysis is that macromolecular breaks down proteins is become micromolecular aminoacid, directly absorbs in order to human body.Produce amino acid whose method and have Hydrochloric Acid Hydrolysis Method, enzyme hydrolysis method, chemical synthesis and microbe fermentation method etc.: Hydrochloric Acid Hydrolysis Method is wherein with a long history, advantage is that production efficiency is high, raw material is cheap and easy to get, equipment is simple, can produce the aminoacid of almost all categories, shortcoming is simultaneously: the hydrochloric acid (1) after hydrolysis is difficult to leave with Amino acid score and remove; The Sal that neutralization produces is too salty as aminoacids solution, adopts the method desalinations such as ion exchange resin very hard, expensive.(2) the smelly difficulty of hydrolysis that hydrochloric acid hydrolysis produces is eliminated, and contaminated environment.Because these these technical barriers can not get solving for a long time, the great potential of Hydrochloric Acid Hydrolysis Method plays not out, has been in the edge be eliminated; Other production methods are just arisen at the historic moment, and are able to large development, but they neither possess the potential technical advantage of Hydrochloric Acid Hydrolysis Method.
The great advantage of enzyme hydrolysis method is mild condition, does not need high temperature, high pressure and acid-resistant system; Shortcoming is that the response time is long, and hydrolysis is not easily complete, and the peptide produced has bad allergy to answer to part human body; Need again special cultivation enzyme condition; Often a kind of enzyme is only hydrolyzed a kind of special peptide tendon; The sad filter of product; Be not as good as Hydrochloric Acid Hydrolysis Method.
Traditional extraction method, enzyme process and chemical synthesis due to the cost of precursor high, complex process, is difficult to the object reaching suitability for industrialized production.
We want the acid hydrolysis adopting other, but acid hydrolysis (comprising hydrochloric acid hydrolysis) method main bugbear is the computational problem by acid amount.Catalytic theory from textbook: hydrolysis constant K=α N δ, α are the catalysic coefficient of acid, and N is molar concentration, and δ becomes positive correlation within the specific limits with hydrolysis temperature T, time t.In other words, hydrolysis effect increases with the increase of the molar concentration N (mol/L) of acid in solution-is directly proportional.
This catalytic theory is defective.When 1. just beginning one's study, we are the same with many people, take for as mentioned above: hydrolysis effect is directly proportional to acid concentration in hydrating solution, using it as a leading indicator.But: in solution, acid concentration not only increases with the increase of sour consumption---and this is conducive to hydrolysis; And increase with the minimizing of amount of water---this does not only increase, and reduces hydrolysis effect on the contrary.Because do not increase by acid amount; Added water is fewer, and the acid concentration of solution is higher, now due to common-ion effect, suppresses mutually, have impact on hydrogen atom ionization, reduces hydrolysis effect.This illustrates that this theory is defective.In fact; Amount of water is very large to hydrolysis potency due, but solution is too dense or too rarely all can reduce hydrolysis effect.2. this theory could not reflect the sour consumption of hydrolysis and the different nitrogen content of certain class hydrolysate (Pteria martensii Dunker, hydriopsis cumingii, scallop, prawn, various Fish ...) relation.3. this theory could not reflect the relation of the sour consumption of hydrolysis and variety classes protein (fish, meat, cartilage, tendon, hair, silk).Therefore, this theory is disabled.
Hydrolysis constant K=α N δ formula accordingly; Present many patents declare or paper when writing acid hydrolysis conditions, usually only write in hydrating solution the molar concentration N being hydrolyzed acid, do not write liquor capacity, how does this know by how many acid amount? this is flicker people!
Summary of the invention
The invention provides and a kind ofly produce the method that Margarita liquid, Margarita ginseng liquid and human body easily absorb Margarita powder, solve the rationale that Margarita layer powder uses; First dissolved pearl powder can improve Margarita hydrolysis efficiency; Margarita and Margarita layer powder are not easily absorbed by the body, original creation citric acid water solution, and first dissolve its calcium carbonate, macromolecular angle glutelin is hydrolyzed into micromolecular aminoacid, and human body can directly absorb; Solve with hydrochloric acid hydrolysis contaminated environment and hydrolysis after acid and salt be difficult to removal problem; Radix Ginseng has strongly invigorating primordial QI and the anti-ageing effect of waiting for a long time, and Margarita aminoacid and Radix Ginseng is combined and brings out the best in each other; Adopt in Calx or Margarita layer powder and easily absorb Margarita with acquisition calcium citrate and human body; Original creation calculates hydrolysis acid amount formula; Find to regulate pH value to improve mouthfeel; Use unique Margarita liquid and Margarita ginseng liquid formula; Derive product estimated output computing formula; Solve a precipitation difficult problem for Margarita liquid and Margarita ginseng liquid.
Technical scheme
Margarita liquid, Margarita ginseng liquid and human body easily absorb Margarita powder processing method, are to adopt citric acid hydrolyzed pearl powder or Margarita layer powder to produce, and comprise the steps:
1, Margarita powder or Margarita layer powder is prepared
Margarita is washed, dries, be ground into 360 order Margarita powder;
Or Margarita (freshwater mussel) shell is ground off coloured top layer, washing, dry, be ground into 360 order Margarita layer powder.
2, dissolving with hydrochloric acid Margarita powder
Margarita powder or Margarita layer powder are inserted in container, the weight filtered Zi Lai Shui ‥ filtered water such as injection, furnishing pasty state, slowly add according to formula several times
[A](I) concentration calculated is 31% food grade salt acid dissolve, till making to reach PH6.4 ~ 6.6.
From formula
[A](I): V
hydrochloric acid 31%=M
margarita powder× 2.0605,
When using 200g Margarita powder or Margarita layer powder, V
hydrochloric acid 31%=M
margarita powder× 2.0605=200 × 2.0605=412.10mL.
In formula: V represents volume, M represents weight, herewith later.
Such as, insert in 5000mL beaker by Margarita powder or Margarita layer powder 200g, inject equivalent filtered Zi Lai Shui ‥ filtered water 200g, furnishing pasty state, slowly adding concentration is several times 31% food grade hydrochloric acid 412.10mL.
First add a small amount of hydrochloric acid, constantly stir, after waiting carbon dioxide bubble roughly to eliminate, add hydrochloric acid solution again.Note preventing foam overflow, if when the foam produced nearly overflows, must step up to stir foam, make lather collapse rapid dispersion, note now not being stirred to bottom, prevent more foams.The nearly 400mL of Margarita powder liquid after dissolving, chloride containing calcium 204g.By a small amount of filtered water, beaker edge bur is washed in beaker.Use nearly 80% just to start to test pH value at hydrochloric acid, reached PH6.4 ~ 6.6 meta-acid state in case reaction is incomplete, stop adding hydrochloric acid.Winter temperature is low, heats a little if desired to promote reaction, but can not be too hot in order to avoid hydrochloric acid volatilization is too much.If add hydrochloric acid to find that there is white pearl powder precipitation, then illustrate that hydrochloric acid consumption is inadequate, need to increase.Hydrochloric acid volatility is very strong, is long placed in and need increases consumption.
3, dilute, filter, abandon liquid
Step 2 solution is injected filtered water, stirs evenly, dilution acid content; With filter paper filtering, the acid that removing is liquid and calcium chloride, then by filtered water, the Margarita angle glutelin on filter paper is thoroughly washed down, for subsequent use.
Such as, the solution for step 2 example injects filtered water to 3000mL, stirs evenly, dilution acid content; With filter paper filtering, the acid that removing is liquid and calcium chloride, then by filtered water, the Margarita angle glutelin on filter paper is thoroughly washed down, for subsequent use.
4, be hydrolyzed
Consult the relevant paper of Margarita can obtain: geneva Margarita layer powder is containing angle glutelin 3.74%; Margarita powder is containing angle glutelin 3.2%; Cristaria plicata Margarita powder is containing angle glutelin 3.4%.Margarita (layer) powder angle glutelin weight [the angle glutelin content of=Margarita (layer) grain weight amount × this kind of Margarita (layer) powder].
Margarita (layer) powder represents Margarita powder or Margarita layer powder, lower same.
Hydrolysis adopts citric acid C
6h
8o
7192.125, commercially available citric acid monohydrate contains citric acid 91.43%, containing water of crystallization 8.57%.General citric acid purity 98%, 2% impurity, now containing citric acid 89.6%, containing water of crystallization 8.4%, is called Fructus Citri Limoniae acid crystals.
To different hydrolysis devices
(1) use 5000mL erlenmeyer flask, adopt citric acid, back hydrolysis Margarita angle glutelin.
In 5000mL erlenmeyer flask, concentrate above 10 ~ 15 parts of (every part of 200g) Margarita (layer) powder angle glutelins, add citric acid as
[B]middle formula (II-1), selects CC=3.0, M
citric acidglutelin weight × 3.0 ,=Margarita (layer) powder angle × glutelin weight × 6.5841,2.1947=Margarita (layer) powder angle, then add water, make totally to reach 3200 ~ 3500mL, back hydrolysis 12 ~ 16 hours.
(2) use 10L stainless steel cauldron, adopt citric acid hydrolyzed pearl angle glutelin.
In 10L stainless steel cauldron, concentrate Margarita (layer) the powder angle glutelin of above 20 ~ 30 parts (every part of 200g), add citric acid as
[B]formula (II-1), selects CC=2.8, M
citric acidglutelin × 2.8 ,=Margarita (layer) powder angle × glutelin × 6.1452,2.1947=Margarita (layer) powder angle.Add appropriate water again, make totally to reach 6400 ~ 7000mL, at 112 ~ 118 DEG C, be hydrolyzed 12 ~ 16 hours.
5, neutralize
To liquid food stage limestone vegetation above, M
calx=M
citric acid× 0.59, see
[C]middle formula (III-1): be first neutralized to PH4.4 ~ 4.6;
Or neutralize with Margarita layer powder, M
margarita layer powder=M
citric acid× 0.8494, see
[C]formula (III-2): be first neutralized to PH4.4 ~ 4.6.
The two is all called A liquid above.
Comparatively reasonable with Margarita layer powder neutralization, because this product is pearl products, the calcium citrate of generation is also Margarita calcium citrate; But Margarita layer powder neutralization reaction is poor, when temperature is low more so, also produce a large amount of carbon dioxide bubbles, pretty troublesome!
6, neutralization is continued, precipitation
A liquid is continued to be neutralized to PH8, abundant reaction precipitation 3 ~ 4 hours.
7, filter paper filtering
Liquid step 6 obtained uses filter paper filtering, obtains liquid B and filtering residue C.
8, liquid B is modulated
1. nitrogen content 0.22 ~ 0.23 (g/100mL or Kg/100L) is adjusted to by the liquid B that step 7 obtains by filtered water.
2. regulate with food grade hydrochloric acid, liquid is reached
[D]pH4.4 ~ 4.6, to improve mouthfeel.
3. the precalculated volume output of product Margarita liquid or Margarita ginseng liquid is pressed
[F]weight percent (g/100mL or Kg/100L), add
[E]the each component of middle batch formula is modulated, and is modulated into Margarita liquid or Margarita ginseng liquid respectively.
9, filter paper filtering
Liquid filter paper filtering after step 8 is modulated, waste.
10, fill, sterilizing
Filling liquid after step 9 being filtered, 100 DEG C of sterilizings 30 minutes, becomes Margarita liquid or Margarita ginseng liquid, containing Margarita aminoacid or Margarita aminoacid and Radix Ginseng extractive solution.
11, calcium citrate calsium supplement
Filtering residue C is dried, pulverize, pack, become calcium citrate calsium supplement.
12, human body easily absorbs Margarita powder
Concentrated by A liquid, dry, pulverize, pack, adult body easily absorbs Margarita powder, and containing Margarita aminoacid and calcium citrate, human body easily absorbs Margarita powder.
Above-mentioned particular content is as follows:
[A] dissolving with hydrochloric acid Margarita powder or Margarita layer powder.Discharged from the encirclement of the calcium carbonate of nearly 24 times of indissolubles by their effective ingredient, we have added this one dissolution process, substantially increase hydrolysis efficiency.The organic acid acidity such as citric acid are too weak, and as dissolving acid reaction not exclusively, the Margarita powder and the gluey Margarita angle glutelin that dissolve the calcium citrate produced etc. and remnants mix, and cannot be separated, can not use." calcium sulfate " precipitation that other mineral acid is formed as: sulfuric acid dissolution Margarita powder also cannot be separated with gluey Margarita angle glutelin, can not use." lime nitrate " that nitric acid dissolve Margarita powder is formed is explosives, can not adopt.Can only use dissolving with hydrochloric acid Margarita powder or Margarita layer powder, Dosage calculation is as follows.
2HCl+CaCO
3——→CaCl
2+2H
2O+CO
2↑
72.922100.089110.99
For ensureing to dissolve completely, require to reach PH6.4 ~ 6.6, hydrochloric acid also has volatilizing loss, and the present increases consumption 10%.All Margarita powder and Margarita layer powder all contain 92% calcium carbonate.Dissolve the HCl needed the same:
M
hCl=(72.922 ÷ 100.089) × 1.1 × M
caCO3=(72.922 ÷ 100.089) × 1.1 × M
margarita powder× 0.92
=M
margarita powder× 0.7373.The food grade hydrochloric acid of present employing seawater electrolysis, concentration 31%, dissolve requirement:
M
hydrochloric acid 31%=M
hCl÷ 0.31=M
margarita powder× 0.7373 ÷ 0.31=M
margarita powder× 2.3784.Being converted into volume is:
V
hydrochloric acid 31%=M
hydrochloric acid 31%÷ d=M
margarita powder× 2.3784 ÷ d=M
margarita powder× 2.3784 ÷ 1.1543=M
margarita powder× 2.0605 ... (I),
Unit in formula: M
margarita powder(g), V
hydrochloric acid 31%(mL); Or M
margarita powder(Kg), V
hydrochloric acid 31%(L).
Formula is applicable to Margarita powder and Margarita layer powder.Concentration of hydrochloric acid is 31% look into book and can obtain density d=1.1543.
[B] acid hydrolysis problem.
1, we are to the derivation of " calculating hydrolysis acid amount formula ".The H of hydrolysis ability only and in hydrolysis acid molecule
+ion is relevant.To polyprotic acid Y, an acid molecule has Y H atom to be ionized into H
+ion.So; Hydrolysis effect is relevant with hydrolysis acid equivalent number.Hydrolysis object is that the nitrogen molecular polypeptide chain in raw material is broken into free amino acid, and raw material nitrogen molecular number is more, needs more hydrolysis acid equivalent numbers, and this is a proportional relation, and being write as equation is:
Hydrolysis acid equivalent number=raw material nitrogen molecular number × CC ... 1., proportionality coefficient aunt CC is called hydrolysis coefficient of intensification.
Hydrolysis acid equivalent number=hydrolysis acid molecule number × Y, ∵ hydrolysis acid molecule number=be hydrolyzed sour weight/hydrolysis acid molecule amount;
Hydrolysis acid equivalent number=hydrolysis acid molecule number × Y=(being hydrolyzed sour weight × Y)/hydrolysis acid molecule amount ... 2.;
Raw material nitrogen molecular number=raw material nitrogen quantity/molecular weight nitroxide 14.0067 ... 3..
Will 2. formula and 3. formula substitute into 1. formula:
(being hydrolyzed sour weight × Y)/hydrolysis acid molecule amount=(raw material nitrogen quantity/14.0067) × CC.
∴ is hydrolyzed sour weight=(hydrolysis acid molecule amount/14.0067) × raw material nitrogen quantity × CC ÷ Y=(hydrolysis acid molecule amount/14.0067) × material protein quality × 16% × CC ÷ Y----(II
0).
Raw material nitrogen quantity=material protein quality × 16%=raw material weight × this kind of material protein content × protein nitrogen content 16%.
The protein nitrogen content of general general meat, fowl, egg, fish, shellfish is 16%, and Margarita imagination is also similar.
Sulphuric acid is binary acid, Y=2.We once used sulphuric acid hydrolysis, because mouthfeel is not good, and danger close, therefore use less as far as possible.
Polyprotic acid except the sulphuric acid overwhelming majority be all weak acid, 2nd ~ 3 yuan of acid not easily ionizable, does not work very much, still regard as unit acid Y=1.Now:
Be hydrolyzed sour weight=(hydrolysis acid molecule amount/14.0067) × material protein quality × 16% × CC----(II).
Wherein, Various material protein content can be found from food composition table or upper the Internet.
The size that hydrolysis coefficient of intensification CC selects with used be hydrolyzed sour catalysic coefficient relevant, also with to be hydrolyzed protein amounts contained by object relevant with type.The catalysic coefficient of strong acid is generally higher than weak acid.Catalysic coefficient α general alignment order is: iodic acid, bromic acid, hydrochloric acid, sulphuric acid, phosphoric acid, oxalic acid, sulfurous acid, citric acid, lactic acid, acetic acid ...When adopting the acid that catalysic coefficient is large, hydrolysis coefficient of intensification CC can with less, and with the acid that catalysic coefficient is little, CC can with large.Scleroprotein composition bone, cartilage, tendon, angle, hair, silk etc., not facile hydrolysis, hydrolysis coefficient of intensification CC can with large.Hydrolysis effect becomes positive correlation with hydrolysis temperature T, time t in general usual range, and also dispel the heat relevant with temperature height and container, but impact is less, these can suitably adjust.Such as: back hydrolysis, temperature is lower, and CC can with larger, and reactor is hydrolyzed, and temperature is higher, and CC can with smaller.Hydrolysis time t is shorter, and CC can with larger; Hydrolysis time t is longer, and CC can with smaller.About the selection of hydrolysis coefficient of intensification CC value.It is for reference that we propose following table from experience, and reader can improve in practice further!
Table 1 is hydrolyzed some reference values of coefficient of intensification CC
# Crusta Penaeus seu Panulirus, containing calcium carbonate, dissolves and needs consumption acids.
2, acid hydrolysis uses and inquires into.Strong acid is mineral acid entirely; Iodate and bromate are dangerous materials or carcinogen; Nitric acid stimulates very much, can explode; After phosphoric acid hydrolysis, after neutralization, reaction is very slow, and precipitation is bonded at chamber wall difficulty and removes, and product is the smell of burning.These acid all can not be used for being hydrolyzed.
The most general is hydrochloric acid, and its catalysic coefficient α is large, can obtain food grade hydrochloric acid from electrolytic seawater, good and cheap.Hydrochloric acid is the composition of human body gastric acid, and the acid after hydrolysis is by soda Na
2cO
3be neutralized into Sal, be well suited for making flavoring agent, mouthfeel is pretty good.Japanese has invented chemical soy sauce with hydrochloric acid hydrolysis Semen sojae atricolor during World War II, propagates its belief on a large scale! But hydrochloric acid hydrolysis is hydrolyzed smelly as consumption too much can produce, we met in Shandong food factory, good unpleasant! The stainless steel cauldron of a 3000L, due to excessive hydrochloric acid, even also has burnt by they! This is the first problem that hydrochloric acid hydrolysis is dealt with improperly.
Before and after 1992 last century, carcinogen---the chloropropyl alcohol content overproof in some soy sauce that China produces, for this reason, European Union's restriction soy sauce exports to European market, large quantities of return of goods.In chloropropyl alcohol, the harm of trichloropropanol to human body is maximum, it is that soybean fat hydrolysis of rupturing under strong acid forms glycerol, and glycerol (glycerol) reacts under strong acid or hot conditions, HCl substituted alcohols hydroxyl and generate chloropropyl alcohol, based on 3-chlorine-1,2-propylene glycol.
Why there will be this problem? this is that the soy sauce of outlet is the blended soy sauce being mixed with hydrochloric acid hydrolysis bean cake mostly because the production efficiency that makes soy sauce is very low.This is the Second Problem that hydrochloric acid hydrolysis deals with appearance improperly.
Besides utilizing hydrochloric acid hydrolysis can cause severe contamination to air, this is a pretty troublesome thing! The stink of hydrochloric acid all is filled the air in the whole workshop of thousands of square metres, extremely feels bad when Shandong food factory produces seafood soy-sauce with hydrochloric acid hydrolysis! This is the 3rd problem that hydrochloric acid hydrolysis occurs.
It is acid after how removing hydrolysis or salt problem that hydrochloric acid hydrolysis produces what also face after aminoacid.Must method deacidification or the salt such as ion exchange resin be adopted after hydrochloric acid hydrolysis, extremely bother.Book is said and can open wide lid heating to drive acid be speak indiscreetly, we almost have dryouied also do not drive how much acid twice, and neutralization produces so how salt and can only be used for producing baste, but too salty as aminoacids solution, and remove salt and be also difficult to, cost is very high!
The catalysic coefficient of sulphuric acid is comparatively large, is again binary strong acid, can remove the heavy metal of product by chelating.Seabed, Zhejiang has " Acaudina molpadioides " of a kind of similar Stichopus japonicus, allegedly the similar Stichopus japonicus of nutrition, but toxicity is large, and containing heavy metal, local fisherman dare not eat.We once became Acaudina molpadioides hydrolyzed solution with sulphuric acid hydrolysis It, and through Zhongshan University, medical college acute toxicity test proves hypotoxicity.Wherein content of beary metal (mg/L): As0.009 is checked through Guangzhou Institute of Analysis; Cd0.052; Hg7.8 × 10
-4; Pb0.18. the food standard of country is all reached.Total number of bacteria, coliform, pathogenic bacterium etc. all reach standard through the inspection of Food Hygiene Supervision and Inspection Institute of Guangdong Province.Aminoacid and taurine summation reach 12815mg/100g, are close with the 14892.44mg/100g of our oyster hydrolyzate.Visible Acaudina molpadioides still has Development volue.Put off until some time later mouthfeel also not so good, sulfurous acid is also like this but sulphuric acid is too strong, accidentally with regard to hurting people or smashing objects, very fearful!
Organic acid only has below secondary strong acid, and catalysic coefficient α is lower, and price is more expensive, is applicable to the aquatic products of the low nitrogen of hydrolysis.Wherein formic acid, acetic acid, propanoic acid threshold value are low, have not only stimulated but also not smelling good; Tartaric acid stimulates very much; Gluconic acid and malic acid price more expensive, catalysic coefficient α is low.We once used citric acid, oxalic acid and lactic acid to be hydrolyzed, and thought that citric acid and oxalic acid comparatively share.α is low for lactic acid catalysic coefficient, expensive again, improper.
3, citric acid and oxalic acid hydrolysis.
Citric acid and oxalic acid hydrolysis are that we initiate, and their catalysic coefficient is slightly low, and price is more expensive than hydrochloric acid, but it is free from environmental pollution, and the acid after hydrolysis can filter out with Calx or Margarita layer powder neutralization, so finished product not saliferous, can produce aminoacids solution, efficiency is very high! This is great advantage.
Measuring with acid with they hydrolysis is below discussed.
(1) citric acid Ca
3(C
6h
5o
7)
2(192.125), be ternary weak acid.Ionization constant K α
1=7.4 × 10
-4, K α
2=1.7 × 10
-5, K α
3=4.0 × 10
-7, imagination only has monoacid to ionize.Add the water of raw material and raw material equivalent in a kettle., and following citric acid amount.From public formula II, adopt citric acid:
M
citric acid=(192.125/14.0067) × material protein quality × 16% × CC
=material protein quality × CC × 2.1947 ... (II-1).
At 112 ~ 118 DEG C, be hydrolyzed 12 ~ 16 hours, imagination only has monoacid to ionize.
(2) oxalic acid H
2c
2o
4(90.036), be binary time strong acid.Ionization constant K α
1=5.9 × 10
-2, K α 2=6.4 × 10
-5, imagination only has monoacid to ionize.Add the water of raw material and raw material equivalent in a kettle., and following oxalic acid amount.From public formula II, adopt oxalic acid:
M
oxalic acid=(90.036/14.0067) × material protein quality × 16% × CC
=material protein quality × CC × 1.0285 ... (II-2).
At 112 ~ 118 DEG C, be hydrolyzed 12 ~ 16 hours.
Oxalic acid at medical industry for the manufacture of chlortetracycline, oxytetracycline, tetracycline, streptomycin, ephedrine.But oxalic acid is harmful, the acid-base value disequilibrium in human body can be made, affect the growth of child; Oxalic acid can also form calcium oxalate precipitation with the calcium of human body and cause kidney or ureteral calculus, very painful; Oxalic acid can form water-soluble complex with many metals.Therefore; Use oxalic acid must be very careful, be hydrolyzed and completely all must become calcium oxalate with limestone vegetation, filter remove clean! All containers must with rustless steel or glass container, in order to avoid form water-soluble complex with some metals.
[C] neutralizes.
1, with food stage limestone vegetation citric acid.Food stage Calx is containing calcium hydroxide 98%.
2C
6H
8O
7+3Ca(OH)
2——→Ca
3(C
6H
5O
7)
2↓+6H
2O
384.251222.284498.443
M
calcium hydroxide=(222.284 ÷ 384.251) × M
citric acid=M
citric acid× 0.5785.
M
calx=M
calcium hydroxide÷ 0.98%=M
citric acid× 0.5785 ÷ 0.98%=M
citric acid× 0.59 ... (III-1)
2, use in Margarita layer powder and citric acid.Margarita layer powder is containing calcium carbonate 92%.
2C
6H
8O
7+3CaCO
3——→Ca
3(C
6H
5O
7)
2↓+3H
2O+3CO
2↑
384.251300.2676498.443
M
caCO3=(300.2676 ÷ 384.251) × M
citric acid=M
citric acid× 0.7814.
∴ M
layer powder=M
caCO3÷ 0.92%=M
citric acid× 0.8494 ... (III-2)
3, with food stage limestone vegetation oxalic acid.
Employing soda neutralizes, and form Disodium oxalate. solvable, not being food additive, is dangerous materials, and this neutralization is unavailable.Or adopt limestone vegetation better.For preventing remaining oxalic acid, must make it fully to precipitate to filter by Zhong He Zhi≤PH8.
H
2C
2O
4+Ca(OH)
2——→CaC
2O
4↓+2H
2O
90.03674.09468128.10012
M
ca (OH) 2=(74.09468 ÷ 90.036) × M
oxalic acid=M
oxalic acid× 0.8229.Food stage Calx is containing calcium hydroxide 98%.
Use food stage Calx: M
calx=M
ca (OH) 2÷ 0.98=M
oxalic acid× 0.8397 ... (III-3).
Adopt in Calx or Margarita layer powder and citric acid, the calcium citrate Ca of acquisition
3(C
6h
5o
7)
2it is the calsium supplement of good absorption.Because in neutral conditions, although its dissolubility is little; But under it arrives the acid condition of human small intestine's tip, absorption of human body is fairly good!
The mouthfeel improvement of [D] product and the adjustment of pH value.
We find: when liquid mouthfeel when acidity is PH4.4-4.6 is best, and antiseptic effect, just engender off-taste to PH6, arrived PH7-8 and be not just very good eating later, this just can smell at N-process later!
The adjustment of pH value.We are when playing initial adjustment, repeatedly add the change of alkali (acid) pH value little, just adjust suddenly excessive, have to acid adding (alkali) recall to again, enough tired! Just find several times that pH value has individual mutation range, investigates interrelated data as following table.
Table 2 HCl of the 0.1N of the NaOH titration 20ml of 0.1N
| Add NaOH (ml) | In and percent | Residue HCl (ml) | Excessive NaOH (ml) | [H +] (M molar concentration) | PH |
| 0.00 | 0.00 | 20.00 | 1.00×10 -1 | 1.00 | |
| 18.00 | 90.00 | 2.00 | 5.26×10 -3 | 2.28 | |
| 19.80 | 99.00 | 0.20 | 5.02×10 -4 | 3.30 | |
| 19.96 | 99.80 | 0.04 | 1.00×10 -4 | 4.00 | |
| 19.98 | 99.90 | 0.02 | 5.00×10 -5 | 4.30 mutation range | |
| 20.00 | 100.00 | 0.00 | 1.00×10 -7 | 7.00 mutation range | |
| 20.02 | 100.1 | 0.02 | 2.00×10- 10 | 9.70 mutation range | |
| 20.04 | 100.2 | 0.04 | 1.00×10- 10 | 10.00 | |
| 21.20 | 106.0 | 1.20 | 2.00×10- 11 | 10.70 | |
| 22.00 | 110.0 | 2.00 | 2.10×10- 12 | 11.70 | |
| 40.00 | 200.0 | 20.00 | 3.00×10- 13 | 12.52 |
From PH1 → PH4.30, the NaOH adding 0.1N is needed to be 19.98ml, and from PH4.31 → PH7.00, only need the NaOH adding 0.1N to be 0.02ml, and from PH7 → PH9.7, only need the NaOH adding 0.1N to be 0.02ml, visible fast in this scope pH value hop, must carefully slowly add.Neutralization for other acid-base class also has analogue!
[E] Margarita liquid and Margarita ginseng liquid product batch formula as follows:
Margarita liquid formula 1:
PH4.4 ~ 4.6, containing amino nitrogen 0.2 ~ 0.21%, tryptophan 0.11% ~ 0.13%, Sal 0.5% ~ 1%, monosodium glutamate 0.05% ~ 0.1%, (I+G) 0.001% ~ 0.002%, Mel 1% ~ 2%, sodium dehydroacetate 0.07% ~ 0.1%.
Margarita liquid formula 2:
PH4.4 ~ 4.6, containing amino nitrogen 0.2 ~ 0.21%, tryptophan 0.11% ~ 0.13%, Mel 1% ~ 3%, sucrose 5% ~ 7%, sodium dehydroacetate 0.07 ~ 1%.
Margarita ginseng liquid formula 1:
PH4.4 ~ 4.6, containing amino nitrogen 0.2 ~ 0.21%, tryptophan 0.11% ~ 0.13%, Sal 0.5% ~ 1%, monosodium glutamate 0.05% ~ 0.1%, (I+G) 0.001% ~ 0.002%, Mel 1% ~ 2%, Radix Ginseng extractive solution 1.8 ~ 2.2% (containing ginsenoside 54 ~ 66mg or g), sodium dehydroacetate 0.07% ~ 0.1%.
Margarita ginseng liquid formula 2:
PH4.4 ~ 4.6, containing amino nitrogen 0.2 ~ 0.21%, tryptophan 0.11% ~ 0.13%, Mel 1% ~ 3%, sucrose 5% ~ 7%, Radix Ginseng extractive solution 1.8 ~ 2.2% (containing ginsenoside 54 ~ 66mg or g), sodium dehydroacetate 0.07 ~ 1%.
Here % still refers to g/100mL or Kg/100L.
Radix Ginseng extractive solution can online purchase.
For diabetes patient, sucrose and Mel can change Sucralose into, and Sucralose sugariness is 600 times of sucrose.Radix Ginseng extractive solution can online purchase.
Acid hydrolysis destroys tryptophan to be needed to add in addition, and alkali hydrolysis method also can be adopted to produce tryptophan.
[F] estimated output V (volume):
By definition extraction ratio γ=product nitrogen content ÷ raw material nitrogen content=[small product size V (mL) × product amino nitrogen (g/mL)] ÷ [raw material weight (g) × this kind of raw material nitrogen content %]
∴ small product size V (mL)=[raw material weight (g) × this kind of raw material nitrogen content % × γ] ÷ product amino nitrogen (g/mL)
∵ raw material nitrogen content %=material protein content % × 16%.The protein nitrogen content of meat, Fish is 16%.
V (mL)=raw material weight (g) × this kind of material protein content % × 16% × γ ÷ product amino nitrogen (g/mL) ... (IV)
From small product size V, measurement products amino nitrogen (g/mL), raw material weight (g) and food composition table can find protein content or the nitrogen content % of raw material, just can calculate extraction ratio γ, generally 60% ~ 65%.
Shi Zhong unit (g, mL, g/mL) can be made into (Kg, L, Kg/L).
The sedimentation problem of [G] product.
In order to improve products taste, when its acidity being adjusted to PH4.4 ~ 4.6, mouthfeel is best, and such product is very naturally merely hit and so far, is now in meta-acid state.Cause product constantly to precipitate, heavily filter after some months, cross some months and precipitate again, so when finish? also do not become with ultrafiltration.Change technique, formula, product still precipitates successively, has consulted my the relevant professor in school (department of chemistry of Ji'nan University) and has all come to nothing.This problem perplexs us for many years, finally just realizes opposition thinking! Does not is not product precipitate successively? do are we afraid of precipitation? that is just acted in a diametrically opposite way, by in the liquid after hydrolysis and to≤PH8, it is allowed to be in alkaline state, the acid salt of citric acid is made to have the sufficient time to be transformed into normal salt precipitation, cross and filter to remove all citric acid normal salt, this just solves sedimentation problem.Be transferred to PH4.4 ~ 4.6 with food grade hydrochloric acid again, solve sensory issues.
From the principle: with citric acid hydrolysis, when limestone vegetation is to PH4.4 ~ 4.6, under being in slant acidity condition, certainly exist the acid salt citric acid 4 hydrogen calcium CaH of a large amount of citric acid
4(C
6h
5o
7)
2with calcium hydrogen citrate CaHC
6h
5o
7, be long placed in and form calcium citrate Ca
3(C
6h
5o
7)
2precipitation and citric acid, as reaction equation below.This is a reversible equilibrium process, both there is citric acid 4 hydrogen calcium or the calcium hydrogen citrate on the formula left side in solution, also there is the calcium citrate precipitation on the right of formula and citric acid.If we filter, the calcium citrate precipitated on the right of formula is removed, just have the citric acid 4 hydrogen calcium on the formula left side or calcium hydrogen citrate at once and again form calcium citrate on the right of formula and citric acid, namely continue to produce precipitation, like this: filter → precipitation → filter → precipitation → filter →, be difficult to stop, our situation about in the past experiencing that Here it is.If we are by liquid Zhong He Zhi≤PH8, constantly neutralize by the citric acid generated on the right of formula, destroy the balance of reaction equation, just do not have to the reaction on the formula left side on the right of formula, the reaction only from the formula left side on the right of formula, reaches new balance, so continue, last citric acid 4 hydrogen calcium or calcium hydrogen citrate become calcium citrate all, remove after filtration, thoroughly solve sedimentation problem! Reaction needed has time enough.
For crustacean hydrolyzed solutions such as shrimps, also can there is a large amount of chitin and be dissolved in acid solution, they also can be precipitated out through long-time storing in the basic conditions, remove by filtering.
When within 1996, we produce scallop seasoning king in Yantai with hydrochloric acid hydrolysis scallop body, use edible alkali Na
2cO
3be neutralized to PH7, be then modulated to PH4.4 ~ 4.6 to improve mouthfeel with citric acid, result product produces a large amount of precipitation, still precipitates, filters time and again, precipitate time and again, cannot solve after filtration.Found it is because scallop body, with not examining clean shell, becomes calcium chloride CaCl through hydrochloric acid hydrolysis afterwards
2, be transferred to PH4.4 ~ 4.6 with citric acid, just define acid salt citric acid 4 hydrogen calcium CaH
4(C
6h
5o
7)
2or calcium hydrogen citrate CaHC
6h
5o
7, so that there is above-mentioned continuous filtration, constantly precipitate! Reaction equation is as 3 formulas below.When this lesson makes us understand to adopt hydrochloric acid hydrolysis, can not to think and improve mouthfeel and use citric acid to regulate pH value, pH value can only be regulated with food grade hydrochloric acid.
2HCl+CaCO
3——→CaCl
2+H
2O+CO
2↑
CaCl
2+2C
6H
8O
7——→CaH
4(C
6H
5O
7)
2+2HCl
CaCl
2+C
6H
8O
7——→CaHC
6H
5O
7+2HCl
Main innovate point of the present invention
1, first the present invention solves the rationale that Margarita layer powder uses, and the prismatic layer of Margarita (freshwater mussel) shell is the aragonite structure of Margarita completely, and the Margarita layer powder made can use as Margarita medical material completely.Overthrown domestic and international textbook, the textbook " Zhang Xi, Qi Zhongyan work--malacology outline " comprising China authority thinks that shell divides three layers, and nexine nacreous layer is the aragonite structure of calcium carbonate; Middle level prismatic layer and top layer horny layer are all calcium carbonate calcite structures----middle level prismatic layer is not the aragonite structure of Margarita, should not work as the conclusion of medical material.
2, dissolved pearl, discharges in the encirclement of the calcium carbonate of Margarita effective ingredient indissoluble, and we have added this one dissolution process, substantially increase hydrolysis efficiency.
3, citric acid hydrolysis is our original creation, and provide the production method of a kind of " Margarita liquid and Margarita ginseng liquid ", by macromolecular for Margarita angle glutelin, be hydrolyzed into micromolecular aminoacid, human body can directly absorb.And solve and be difficult to removal problem with the acid after surplus after the contaminated environment of hydrochloric acid hydrolysis and hydrolysis and salt.Now and afterproduct be calcium citrate, be easy to isolated by filtration.
4, create
[B]calculate hydrolysis acid amount formula.
5, adopt in Calx or Margarita layer powder and obtain calcium citrate calsium supplement and human body easily absorbs Margarita powder
6, product is improved
[D]mouthfeel.A liquid is adjusted to PH4.4 ~ 4.6, improves the mouthfeel that human body easily absorbs Margarita; Liquid B is pressed
[E]recipe requirements is adjusted to PH4.4 ~ 4.6, improves the mouthfeel of Margarita liquid and Margarita ginseng liquid.
7, unique salty, sweet four kinds of Margarita liquids and Margarita ginseng liquid formula is used
[E].
Both Margarita and Radix Ginseng are combined, bring out the best in each other!
8, the computing formula of product estimated output is derived
[F].
9, a precipitation difficult problem for Margarita liquid and Margarita ginseng liquid product is solved
[G].Liquid B continues to be neutralized to PH8 at A liquid, and abundant reaction precipitation 3 ~ 4 hours, filter paper filtering obtains.Do not contain the acid salt of citric acid as citric acid 4 hydrogen calcium CaH
4(C
6h
5o
7)
2with calcium hydrogen citrate CaHC
6h
5o
7, can not precipitate again.We are in JIUYUE, the 2003 prawn head hydrolyzed solution product of 19 days--" red prawn juice ", limpid bright so far, have no precipitation!
10, enter industrialized reactor technology for hydrolyzing from the back hydrolysis technology of laboratory, this is a leap.Have many new techniques to lack this leap exactly, rest on laboratory all the time, cannot promote!
Detailed description of the invention
Below in conjunction with specific embodiment, the invention will be further described:
Embodiment 1
Use 5000mL erlenmeyer flask, adopt the angle glutelin of citric acid back hydrolysis 2000g geneva Margarita layer powder, produce " geneva Margarita liquid, geneva Margarita ginseng liquid and human body easily absorb geneva Margarita powder ".Geneva Margarita layer powder is containing angle glutelin 3.74%.
200g geneva Margarita layer powder is added hydrochloric acid as
[A]middle public formula I: V
hydrochloric acid 31%=M
geneva Margarita layer powder× 2.0605=200 × 2.0605=412.10mL, makes it dissolve, till reaching PH6.4 ~ 6.6.Be diluted to 3000mL, filter paper filtering, abandon liquid.By filtered water, the Margarita angle glutelin on filter paper is thoroughly washed down again.Merge the angle glutelin that 10 parts are total to 2000g geneva Margarita layer powder.
Calculate hydrolysis citric acid.From
[B]middle formula (II-1), selects CC=3.0:
M
citric acid=material protein quality × 3.0 × 2.1947=material protein quality × 6.5841.
Geneva Margarita layer powder is containing angle glutelin 3.74%, M
glutelin=M
geneva nacreous layer× 3.74%=2000 × 3.74%=74.8g.
M
citric acid=74.8 × 6.5841=492.49g.M
fructus Citri Limoniae acid crystals=M
citric acid÷ 0.896=549.65.Neutralization
From
[C]middle formula (III-1) calculates neutralization food stage Calx: M
calx=0.59 × M
citric acid=0.59 × 492.49=290.57g.
Or formula (III-2) calculates neutralization Margarita layer powder: M
margarita layer powder=0.8494 × M
citric acid=0.8494 × 492.49=418.32g.Estimated output V (volume), is shown in
[F]preceding formula (IV):
V (mL)=[raw material weight (g) × this kind of material protein content % × 16% × 65% (extraction ratio)] ÷ product nitrogen content (g/mL).=2000g × 3.74% × 16% × 65% (extraction ratio) ÷ 0.002 (g/mL)=3889.6 (mL).
Geneva Margarita liquid and geneva Margarita ginseng liquid nitrogen content 0.002 (g/mL).
Charging: Σ=geneva Margarita angle glutelin 74.8g+ water 2600mL+M
fructus Citri Limoniae acid crystals.
In 5,000mL erlenmeyer flask, inject above geneva Margarita layer powder angle glutelin 74.8g, Fructus Citri Limoniae acid crystals 549.65g, then add 2600mL water.Back hydrolysis 12 ~ 16 hours.
Being neutralized to PH4.4 ~ 4.6 with food stage Calx 290.57g or Margarita layer powder 418.32g, is be A liquid.A liquid is continued to be neutralized to PH8, abundant reaction precipitation 3 ~ 4 hours.Filter paper filtering obtains liquid B and filtering residue C.By precalculated volume output
[F]weight percent (g/100mL or Kg/100L), add
[E]the each component of middle batch formula is modulated.Filter paper filtering, remove slag, liquid vial fill, 100 DEG C of sterilizings 30 minutes, becomes geneva Margarita liquid or geneva Margarita ginseng liquid, containing geneva Margarita aminoacid or geneva Margarita aminoacid and Radix Ginseng extractive solution.Filtering residue C is dried, pulverizes, be packaged into calcium citrate calsium supplement.Concentrated by A liquid, dry, pulverize, packaging becomes geneva human body and easily absorbs Margarita powder, containing geneva Margarita aminoacid and calcium citrate.
Embodiment 2
Use 10L stainless steel cauldron, adopt the angle glutelin of citric acid hydrolysis 5000g Margarita powder, produce " Margarita liquid, Margarita ginseng liquid and human body easily absorb Margarita powder ".Margarita is containing angle glutelin 3.2%.
200g Margarita powder is added hydrochloric acid as
[A]middle public formula I: V
hydrochloric acid 31%=M
hat freshwater mussel Margarita powder× 2.0605=200 × 2.0605=412.10mL, makes it dissolve, till reaching PH6.4 ~ 6.6.Be diluted to 3000mL, filter paper filtering, abandon liquid.By filtered water, the Margarita angle glutelin on filter paper is thoroughly washed down again.Merge the angle glutelin that 25 parts are total to 5000g Margarita powder.
Calculate hydrolysis citric acid.From
[B]middle formula (II-1), selects CC=2.8:
M
citric acid=material protein quality × 2.8 × 2.1947=material protein quality × 6.1452.
Margarita is containing angle glutelin 3.2%, M
glutelin=5000 × 3.2%=160g.
M
citric acid=160 × 6.1452=983.23g.M
fructus Citri Limoniae acid crystals=M
citric acid÷ 0.896=1097.35g.
From
[C]middle formula (III-1) calculates neutralization food stage Calx: M
calx=0.59 × M
citric acid=0.59 × 983.23=580.1g.
Or formula (III-2) calculates neutralization Margarita layer powder: M
margarita layer powder=0.8494 × M
citric acid=0.8494 × 983.23=835.16g.
Estimated output V (volume), is shown in
[F]middle public formula IV:
V (mL)=[raw material weight (g) × this kind of material protein content % × 16% × 65% (extraction ratio)] ÷ product nitrogen content (g/mL).=5000g × 3.2% × 16% × 65% (extraction ratio) ÷ 0.002 (g/mL)=8320 (mL).
Margarita powder liquid or Margarita powder ginseng liquid nitrogen content 0.002 (g/mL).
Charging: Σ=Margarita powder angle glutelin 160g+ water 2600mL+M
fructus Citri Limoniae acid crystals.
In 10L stainless steel cauldron, inject above Margarita angle glutelin 160g, Fructus Citri Limoniae acid crystals 1097.35g, then add 5200mL water.112 ~ 118 DEG C of hydrolysis 12 ~ 16 hours.
Being neutralized to PH4.4 ~ 4.6 with food stage Calx 580.1g or with Margarita layer powder 835.16g, is be A liquid.A liquid is continued to be neutralized to PH8, abundant reaction precipitation 3 ~ 4 hours.Filter paper filtering obtains liquid B and filtering residue C.Liquid B is pressed precalculated volume output
[F]weight percent (g/100mL or Kg/100L), add
[E]the each component of middle batch formula is modulated.Filter paper filtering, waste, liquid vial fill, 100 DEG C of sterilizings 30 minutes, becomes Margarita liquid or Margarita ginseng liquid, containing Margarita aminoacid or Margarita aminoacid and Radix Ginseng extractive solution.Filtering residue C is dried, pulverizes, be packaged into calcium citrate calsium supplement.Concentrated by A liquid, dry, pulverize, packaging becomes hydriopsis cumingii human body and easily absorbs Margarita powder, containing Margarita aminoacid and calcium citrate.
Embodiment 3
Use 10L stainless steel cauldron, adopt the angle glutelin of citric acid hydrolysis 5000g cristaria plicata Margarita powder, produce " cristaria plicata Margarita liquid, cristaria plicata Margarita ginseng liquid and human body easily absorb cristaria plicata Margarita powder ".Cristaria plicata Margarita is containing angle glutelin 3.4%.
200g cristaria plicata Margarita powder is added hydrochloric acid as
[A]middle public formula I: V
hydrochloric acid 31%=M
hat freshwater mussel Margarita powder× 2.0605=200 × 2.0605=412.10mL, makes it dissolve, till reaching PH6.4 ~ 6.6.Be diluted to 3000mL, filter paper filtering, abandon liquid.By filtered water, the Margarita angle glutelin on filter paper is thoroughly washed down again.Merge the angle glutelin that 25 parts are total to 5000g cristaria plicata Margarita powder.
Calculate hydrolysis citric acid.From
[B]middle formula (II-1), selects CC=2.8:
M
citric acid=material protein quality × 2.8 × 2.1947=material protein quality × 6.1452.
Cristaria plicata Margarita is containing angle glutelin 3.4%, M
glutelin=5000 × 3.4%=170g.
M
citric acid=170 × 6.1452=1044.68g.M
fructus Citri Limoniae acid crystals=M
citric acid÷ 0.896=1165.94g.
From
[C]middle formula (III-1) calculates neutralization food stage Calx: M
calx=0.59 × M
citric acid=0.59 × 1044.68=616.36g.
Or formula (III-2) calculates neutralization Margarita layer powder: M
margarita layer powder=0.8494 × M
citric acid=0.8494 × 1044.68=887.35g.
Estimated output V (volume), is shown in
[F]middle public formula IV:
V (mL)=[raw material weight (g) × this kind of material protein content % × 16% × 65% (extraction ratio)] ÷ product nitrogen content (g/mL).=5000g × 3.4% × 16% × 65% (extraction ratio) ÷ 0.002 (g/mL)=8840 (mL).
Cristaria plicata Margarita liquid or cristaria plicata Margarita ginseng liquid nitrogen content 0.002 (g/mL).
Charging: Σ=cristaria plicata Margarita angle glutelin 170g+ water 5200mL+M
fructus Citri Limoniae acid crystals.
In 10L stainless steel cauldron, inject above cristaria plicata Margarita angle glutelin 170g, Fructus Citri Limoniae acid crystals 1165.93g, then add 5200mL water.112 ~ 118 DEG C of hydrolysis 12 ~ 16 hours.
Being neutralized to PH4.4 ~ 4.6 with food stage Calx 616.36g or with Margarita layer powder 887.35g, is be A liquid.A liquid is continued to be neutralized to PH8, abundant reaction precipitation 3 ~ 4 hours.Filter paper filtering obtains liquid B and filtering residue C.Liquid B is pressed precalculated volume output
[F]weight percent (g/100mL or Kg/100L), add
[E]the each component of middle batch formula is modulated.Filter paper filtering, waste, liquid vial fill, 100 DEG C of sterilizings 30 minutes, becomes cristaria plicata Margarita liquid or cristaria plicata Margarita ginseng liquid, containing cristaria plicata Margarita aminoacid or cristaria plicata Margarita aminoacid and Radix Ginseng extractive solution.Filtering residue C is dried, pulverizes, be packaged into calcium citrate calsium supplement.Concentrated by A liquid, dry, pulverize, packaging becomes cristaria plicata human body and easily absorbs Margarita powder, containing cristaria plicata Margarita aminoacid and calcium citrate.
Claims (7)
1. Margarita liquid, Margarita ginseng liquid and human body easily absorb Margarita powder processing method, are characterised in that and adopt citric acid hydrolyzed pearl powder to produce, comprise the steps:
(1) prepare Margarita powder and Margarita layer powder
Margarita is washed, dries, be ground into 360 order Margarita powder;
Or Margarita (freshwater mussel) shell grinds off coloured top layer, washing, dries, is ground into 360 order Margarita layer powder;
(2) dissolving with hydrochloric acid
Insert in container by above Margarita powder or Margarita layer powder, the weight filtered water such as injection, furnishing pasty state, slowly adding the concentration calculated according to public formula I is several times 31% food grade salt acid dissolve, till making to reach PH6.4 ~ 6.6;
From public formula I: V
hydrochloric acid 31%=M
margarita powder× 2.0605
In formula: V represents volume, M represents weight;
(3) dilute, filter
Step 2 solution is injected filtered water, stirs evenly, dilution acid content, with filter paper filtering, abandons liquid, is more thoroughly washed down by the Margarita angle glutelin on filter paper by filtered water, for subsequent use;
(4) citric acid hydrolysis
Adopt citric acid C
6h
8o
7the angle glutelin of 192.125 hydrolyzed pearl powders or Margarita layer powder;
(5) neutralize
The liquid food stage Calx of step 4 or Margarita layer powder are neutralized to PH4.4 ~ 4.6, obtain A liquid;
(6) continue neutralization, precipitation
A liquid is continued to be neutralized to PH8, precipitates 3 ~ 4 hours;
(7) filter paper filtering
Liquid step 6 obtained uses filter paper filtering, obtains liquid B and filtering residue C;
(8) modulate liquid B
Nitrogen content 0.22 ~ 0.23 (g/100mL or Kg/100L) is adjusted to by the liquid B that step 7 obtains by filtered water;
Regulate with food grade hydrochloric acid, make liquid reach PH4.4 ~ 4.6;
By the weight percent of the precalculated volume output of product Margarita liquid or Margarita ginseng liquid, add each component of batch formula and modulate, be modulated into Margarita liquid or Margarita ginseng liquid respectively;
(9) filter paper filtering
(10) fill, sterilizing
Filling liquid after step 9 being filtered, 100 DEG C of sterilizings 30 minutes, becomes Margarita liquid or Margarita ginseng liquid, containing Margarita aminoacid or Margarita aminoacid and Radix Ginseng extractive solution.
2. easily absorb Margarita powder processing method according to Margarita liquid according to claim 1, Margarita ginseng liquid and human body, it is characterized in that
Also comprise following steps
(11) calcium citrate calsium supplement
Filtering residue C dried, pulverizes, be packaged into calcium citrate calsium supplement.
3. easily absorb Margarita powder processing method according to Margarita liquid according to claim 2, Margarita ginseng liquid and human body, it is characterized in that
Also comprise following steps
(12) human body easily absorbs Margarita powder
Concentrated by A liquid, dry, pulverize, pack, adult body easily absorbs Margarita powder, and containing Margarita aminoacid and calcium citrate, human body easily absorbs Margarita powder.
4. easily absorb Margarita powder processing method according to Margarita liquid according to claim 1, Margarita ginseng liquid and human body, it is characterized in that
With in dissolving with hydrochloric acid Margarita powder or Margarita layer powder step, by 200g Margarita powder or Margarita layer powder, insert in 5000mL beaker, inject equivalent filtered water 200g, furnishing pasty state, slowly adding concentration is several times that 31% food grade hydrochloric acid makes 412.10mL;
First add a small amount of hydrochloric acid, constantly stir, after waiting carbon dioxide bubble to eliminate, add hydrochloric acid again; The nearly 400mL of Margarita powder liquid after dissolving, chloride containing calcium 204g, used nearly 80% just to start to test pH value at hydrochloric acid, reaches PH6.4 ~ 6.6 meta-acid state in case reaction is incomplete, stop adding hydrochloric acid.
5. easily absorb Margarita powder processing method according to Margarita liquid according to claim 1, Margarita ginseng liquid and human body, it is characterized in that citric acid is hydrolyzed
Citric acid C
6h
8o
7192.125, use 5000mL erlenmeyer flask back hydrolysis
In 5000mL erlenmeyer flask, concentrate above 10 ~ 15 parts of (every part of 200g) Margarita (layer) powder angle glutelins, add citric acid, M
citric acidglutelin weight × 3.0 ,=Margarita (layer) powder angle × glutelin weight × 6.5841,2.1947=Margarita (layer) powder angle, then add water, make totally to reach 3200 ~ 3500mL, back hydrolysis 12 ~ 16 hours.
6. easily absorb Margarita powder processing method according to Margarita liquid according to claim 1, Margarita ginseng liquid and human body, it is characterized in that
Use the hydrolysis of 10L stainless steel cauldron citric acid
In 10L stainless steel cauldron, concentrate Margarita (layer) the powder angle glutelin of above 20 ~ 30 parts (every part of 200g), add citric acid, M
citric acidglutelin × 2.8 ,=Margarita (layer) powder angle × glutelin × 6.1452,2.1947=Margarita (layer) powder angle.Add appropriate water again, make totally to reach 6400 ~ 7000mL, at 112 ~ 118 DEG C, be hydrolyzed 12 ~ 16 hours.
7. easily absorb Margarita powder processing method according to Margarita liquid according to claim 1, Margarita ginseng liquid and human body, it is characterized in that
To liquid food stage limestone vegetation above, M
calx=M
citric acid× 0.59, be first neutralized to PH4.4 ~ 4.6;
Or neutralize with Margarita layer powder, M
margarita layer powder=M
citric acid× 0.8494, be first neutralized to PH4.4 ~ 4.6; The two is all called A liquid above.
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