CN105165308A - Cultivation method for ligusticum wallichii - Google Patents
Cultivation method for ligusticum wallichii Download PDFInfo
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- CN105165308A CN105165308A CN201510427406.2A CN201510427406A CN105165308A CN 105165308 A CN105165308 A CN 105165308A CN 201510427406 A CN201510427406 A CN 201510427406A CN 105165308 A CN105165308 A CN 105165308A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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Abstract
The invention discloses a cultivation method for ligusticum wallichii. The method comprises the following steps: a, soil treatment: uniformly mixing quicklime and sodium polyacrylate with surface soil, wherein the usage amount of quicklime is 100 kg/mu and the usage amount of sodium polyacrylate is 150 kg/mu; and b, cultivating ligusticum wallichii seeds. The cultivation territory is Yongshou Town, Meishan City, Sichuan province, and the longitude of the territory is east longitude 03 degrees, 50'51.46'' and the latitude is northern latitude 29 degrees, 55'42.65''. The method disclosed by the invention can be used for remarkably reducing the content of cadmium of ligusticum wallichii and meanwhile improving the content of active components of ligusticum wallichii as well as the output of ligusticum wallichii, and is good application prospect.
Description
Technical field
The present invention relates to a kind of cultivation method of Ligusticum wallichii.
Background technology
Ligusticum wallichii is the dry rhizome of samphire Ligusticum wallichii LigusticumchuanxiongHort..For chest impediment and cardialgia, the shouting pain of the chest side of body, tumbling and swelling, irregular menstruation, through closing dysmenorrhoea ， Disorder lump in the abdomen stomachache, headache, arthralgia due to wind-dampness.Modern pharmacodynamic study proves that Ligusticum wallichii mainly has affects female reproductive system, cardiovascular system, nervous system, respiratory system, digestive system, analgesia, anti-inflammatory, antipyretic, effect such as antitumor grade.Volatile oil is one of main active of Ligusticum wallichii, can coronary artery dilator, increases CF, also there is spasmolysis and refrigeration function, uterus, intestines and stomach and vascular smooth muscle contraction can be suppressed, reduce blood pressure, there is calmness, analgesia, spasmolysis, the effect such as anticonvulsion.Therefore, the volatile oil content improving Ligusticum wallichii when cultivating is significant.
In recent years the demand of market to Ligusticum chuanxiong Hort is increase tendency, but, because Ligusticum chuanxiong Hort market price fluctuations causes greatly the enthusiasm of Cotton Varieties by Small Farming Households Ligusticum wallichii to decline, and then cause Ligusticum wallichii output can not meet the demand in market, be badly in need of wanting finding method to improve the output of Ligusticum wallichii.
Simultaneously, along with people more and more pay close attention to food and pharmaceutical safety, test stone for food and medicine also becomes all the more harsh, and the bulk medicinal materials outlet of Ligusticum wallichii all the time as China's Chinese herbal medicine class is American-European, but because its heavy metal cadmium content exceeds standard since the nineties in 20th century, the drug safety that have impact on Ligusticum wallichii is kept outside of the door by multiple country, has also had a strong impact on its foreign exchange earning.Therefore, the cultivation method of research reduction Ligusticum wallichii cadmium content is very urgent.
The happiness of Ligusticum wallichii plant is had a moderate climate, rainfall is abundant, sunshine is sufficient and more moistening, the environment of the subtropics monsoon climate that day and night temperature is large.Deep at soil layer, loose fertile, draining is good, the content of organic matter is abundant, well-grown in neutrality or slightly acidic sand loam.Ligusticum wallichii is planted in the beginning of autumn of around in early August usually, gathers about the first tenday period of a month May next year, 280 ~ 290 days vegetative period.
Summary of the invention
The invention provides a kind of cultivation method of Ligusticum wallichii, output and the active component content of Ligusticum wallichii can be improved simultaneously, the cadmium content of Ligusticum wallichii can also be reduced.
The present invention reduces the cultivation method of Ligusticum wallichii cadmium content, comprises the following steps:
A, soil treatment: preparing quick lime, Sodium Polyacrylate, mix with table soil, the usage amount of quicklime is 100kg/ mu, and the usage amount of Sodium Polyacrylate is 150kg/ mu;
B, get Ligusticum wallichii Siberian cocklebur kind, cultivation, wherein, the region of cultivation is Yongshou town, Meishan city, and its longitude is east longitude 03 ° 50 ' 51.46 ", latitude is north latitude 29 ° 55 ' 42.65 ".
Table soil, refer to the top of earth, thickness is 5 ~ 10cm.
Preferably, in step b, the step of described cultivation is as follows:
(1) Ligusticum wallichii Siberian cocklebur kind is got, sowing;
(2) fill the gaps with seedlings, intertill and clean tillage, fertilising;
(3) gather, dry.
Preferably, in step (1), the time of described sowing is early or mid September.
Preferably, in step (1), described Ligusticum wallichii Siberian cocklebur kind seeding quantity is every mu of 30 ~ 40kg.
In step (2), the mode of fertilising is:
Mid-September and mid-October, respectively use 1 urea, consumption is 15kg/ mu; Late October, to mid-November, uses 2 liquefied ammonia, and consumption is 15-20kg/ mu; Late November-late December, use 2 composite fertilizers and urea, composite fertilizer's consumption is 14-18kg/ mu, and amount of urea is 10kg/ mu.
After turning green in 2 ~ March of next year, then enrich 1 composite fertilizer and urea, composite fertilizer's consumption is 14-18kg/ mu, and amount of urea is 10kg/ mu.
Turn green, refer to plant seedling replanting or after surviving the winter, become green from yellow, and restoration ecosystem.
The present invention combines the interpolation quicklime of specified quantitative and the Sodium Polyacrylate of specified quantitative at seeding time, and significantly can reduce the cadmium content of Ligusticum wallichii, improve the content of the active component of Ligusticum wallichii and the output of Ligusticum wallichii, application prospect is good simultaneously.
The embodiment of form by the following examples, is described in further detail foregoing of the present invention.But this should be interpreted as that the scope of the above-mentioned theme of the present invention is only limitted to following embodiment.All technology realized based on foregoing of the present invention all belong to scope of the present invention.
Accompanying drawing explanation
The chromatogram of Fig. 1 forulic acid reference substance
The chromatogram of Fig. 2 Ligusticum wallichii test sample
Embodiment
Embodiment 1 Ligusticum wallichii cultivation method of the present invention
1 material
Ligusticum wallichii Siberian cocklebur kind derives from small fish hole town, Pengzhou City, Sichuan Province.Quicklime is purchased from Emeishan City nine Li Xingmin pulverized limestone factory; ; Sodium Polyacrylate (food-grade, purchased from Chengdu Yi Hao chemical products Co., Ltd).
Composite fertilizer (N-P
2o
5-K
2o:16-16-16, Sichuan Mei Feng and clay fertilizer research and development centre of the Chinese Academy of Agricultural Sciences joint research and development); Urea (total nitrogen>=46.4%, Sichuan Meifeng Chemical Co., Ltd.); Liquid nitrogen (total nitrogen>=32%, Sichuan Meifeng Chemical Co., Ltd.).
2. region
Yongshou town, Meishan city, north latitude 29 ° 55 ' 42.65 ", east longitude 03 ° 50 ' 51.46 ".
3 methods
3.1 experiment groupings
Be divided into 2 groups, each group of area under cultivation is plot area a: 10m
2, often group establish 3 times parallel.Before Ligusticum wallichii sowing, quicklime, Sodium Polyacrylate and table soil (are shown soil, refer to the top of earth, thickness is 5 ~ 10cm) Homogeneous phase mixing, wherein the addition of quicklime is 100kg/ mu, the addition of Sodium Polyacrylate is 150kg/ mu, not add the soil of quicklime and Sodium Polyacrylate for control group.
3.2 cultivation method
3.2.1 field seeding
3.2.1.1 carefully reject again before Siberian cocklebur (Siberian cocklebur i.e. Siberian cocklebur kind) process sowing and have upper Siberian cocklebur sprouted without bud or bud of worm channel, joint dish hollow and joint, then Siberian cocklebur is made seed soaking, plant with under 50% carbendazim and thiophanate methyl 500 times of immersion kind 5-10min.
3.2.1.2 early or mid September in sowing time.
3.2.1.3 before sowing, quicklime, Sodium Polyacrylate mix with table soil by land treatment systems.
3.2.1.4 seeding row spacing 25cm × 30cm is pressed in type of seeding, is upwards buried by have gentle hands light press by Siberian cocklebur bud head, touches soil and is advisable, expose half and show at soil to save dish.After cultivation, with the joint dish of rice straw mulching Siberian cocklebur.Every mu with Siberian cocklebur 30 ~ 40kg.
3.2.2 field management
3.2.2.1 fill the gaps with seedlings for being short of seedling, bad seedling, can the cloudy day be selected, take Siberian cocklebur for subsequent use and fill the gaps with seedlings, balled transplanting, must water after benefit.The work of filling the gaps with seedlings must terminate before the Autumnal Equinox.
3.2.2.2 after intertill and clean tillage early September neat seedling, shallow hoe intertillage 1 time and will Ligusticum wallichii in the ranks mulching straw (shallow hoe intertillage refers to the surface tillage adopting the instruments such as hand hoe to carry out between Ligusticum wallichii plant, have loosen the soil, weeding, the effect that heats, preserve soil moisture).In 1 month next year, the last ten-days period, when ground cauline leaf starts the withered and yellow time of falling seedling, withering cauline leaf in cleaning field, and earths up, and producing region claims " ulling up winter medicine ".
3.2.2.3 rich water quality management controls with growth
Mid-September and mid-October, respectively use 1 urea, consumption is 15kg/ mu; Late October, to mid-November, uses 2 liquefied ammonia, and consumption is 15-20kg/ mu; Late November-late December, use 2 composite fertilizers and urea, composite fertilizer's consumption is 14-18kg/ mu, and amount of urea is 10kg/ mu.
After turning green in 2 ~ March of next year, then enrich 1 composite fertilizer and urea, composite fertilizer's consumption is 14-18kg/ mu, and amount of urea is 10kg/ mu.
3.2.3 pick and process
3.2.3.1 late April of Second Year after cultivation of gathering gathers.Select fine day, dig out rhizome, shake off earth, removing cauline leaf.Slightly to dry in the air in field aqueous vapor, transport drying back.
3.2.3.2 Habitat producing is dried.Baking a heatable brick bed process strictly controls the temperature on a heatable brick bed bed, and medicinal material place temperature must not more than 70 DEG C.Turn over baking every day once, half-dried stem tuber is taken out, is placed in and hits the container made of bamboo, wicker, ratten, etc., the earth having hit dry and fibrous root.During continuous baking, fresh stem tuber is put in lower floor, and upper strata is put half-dried, and after upper strata has part absolutely dry, then point levels is respectively hit once, eliminates earth and fibrous root, selects absolutely dry, is commodity.Not dry continuation is dried, till absolutely dry.
3.3 detect
1 increment product are adopted in each community, and every increment product get 30 strains at random.Unification selects impurity elimination after gathering, section, dries, and pulverizes, and crosses 60 mesh sieves, makes medicinal powder, and trust academy of agricultural sciences of Sichuan Province has carried out heavy metal Cadmium detrmination.
Ethanol soluble extractives assay measures according to method under " Chinese Pharmacopoeia " 2010 editions Ligusticum wallichii items.
Volatile oil content testing extracts with reference to " Chinese Pharmacopoeia " 2010 editions one annex XD second method steam distillation and measures.
Ferulaic acid content measures and measures with reference to method under " Chinese Pharmacopoeia " 2010 editions Ligusticum wallichii items.
Chromatographic condition chromatographic column is KromasilC18 (5 μm, 250mm × 4.6mm): mobile phase is methyl alcohol-1% acetum (30:70); Determined wavelength 321nm; Flow velocity 1.0mlmin
-1, column temperature 30 DEG C, sample size 10 μ l.The preparation precision of reference substance solution takes forulic acid reference substance 100 μ g, puts in the brown volumetric flask of 5ml, adds 70% methyl alcohol and dissolve and be settled to scale, shake up, and obtaining concentration is 20.0 μ gml
-1reference substance solution.
Each sample medicinal powder (crossing No. four sieves) 0.5g is got in the preparation of sample solution, accurately weighed, puts in tool plug conical flask, precision adds 70% methyl alcohol 50ml, weighed weight after close plug, heating water bath backflow 30min, let cool, more weighed weight, the weight of less loss is supplied with 70% methyl alcohol, shake up, leave standstill, get supernatant, miillpore filter (0.45 μm) filters, get subsequent filtrate, to obtain final product.
The drafting precision of calibration curve draws reference substance solution 2,5,10,15,20 μ l sample introduction, and with forulic acid integrating peak areas value for ordinate, its content is abscissa, drawing standard curve.Regression equation is y=27.8848648x-1.1489417, r=0.9999, and ferulaic acid content is good linear relation with integrating peak areas value within the scope of 0.04 ~ 0.50 μ g.
Sample size measures accurate pipette samples solution 10 μ l sample introduction, by above-mentioned chromatographic condition working sample content.
The chromatogram of forulic acid reference substance and the chromatogram of Ligusticum wallichii test sample are as Fig. 1 ~ Fig. 2.
Data acquisition MicrosoftExce12013 and SPSS13 carries out adding up and Treatment Analysis.
4 testing results
Through detecting, result is as shown in the table:
Table 1 quicklime, Sodium Polyacrylate process experimental result (
n=3)
The results show, adopts the present invention to combine the mode of adding 100kg/ mu quicklime and 150kg/ mu Sodium Polyacrylate, effectively can reduce the cadmium content of Ligusticum wallichii, can significantly improve the output of Ligusticum wallichii and the active component content of Ligusticum wallichii simultaneously.
Applicant is in previous experiments, in other regions, e.g., Ao Ping town, Pengzhou City, Sichuan Province adopts the mode of combining interpolation 100kg/ mu quicklime and 150kg/ mu Sodium Polyacrylate to cultivate, then not only can not improve the active component content of Ligusticum wallichii, the active component content of Ligusticum wallichii can be reduced on the contrary.
To sum up, the present invention combines the interpolation quicklime of specified quantitative and the Sodium Polyacrylate of specified quantitative at seeding time, and significantly can reduce the cadmium content of Ligusticum wallichii, improve the content of the active component of Ligusticum wallichii and the output of Ligusticum wallichii, application prospect is good simultaneously.
Claims (5)
1. a cultivation method for Ligusticum wallichii, is characterized in that: comprise the following steps:
A, soil treatment: preparing quick lime, Sodium Polyacrylate, mix with table soil, the usage amount of quicklime is 100kg/ mu, and the usage amount of Sodium Polyacrylate is 150kg/ mu;
B, get Ligusticum wallichii Siberian cocklebur kind, cultivation, wherein, the region of cultivation is Yongshou town, Meishan city, and its longitude is east longitude 03 ° 50 ' 51.46 ", latitude is north latitude 29 ° 55 ' 42.65 ".
2. cultivation method according to claim 1, is characterized in that: in step b, and the step of described cultivation is as follows:
(1) Ligusticum wallichii Siberian cocklebur kind is got, sowing;
(2) fill the gaps with seedlings, intertill and clean tillage, fertilising;
(3) gather, dry.
3. cultivation method according to claim 2, is characterized in that: in step (1), and the time of described sowing is early or mid September.
4. cultivation method according to claim 2, is characterized in that: in step (1), and described Ligusticum wallichii Siberian cocklebur kind seeding quantity is every mu of 30 ~ 40kg.
5. cultivation method according to claim 2, is characterized in that: in step (2), the mode of fertilising is:
Mid-September and mid-October, respectively use 1 urea, consumption is 15kg/ mu; Late October, to mid-November, uses 2 liquefied ammonia, and consumption is 15-20kg/ mu; Late November-late December, use 2 composite fertilizers and urea, composite fertilizer's consumption is 14-18kg/ mu, and amount of urea is 10kg/ mu.
After turning green in 2 ~ March of next year, then enrich 1 composite fertilizer and urea, composite fertilizer's consumption is 14-18kg/ mu, and amount of urea is 10kg/ mu.
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| CN201510427406.2A CN105165308A (en) | 2015-07-20 | 2015-07-20 | Cultivation method for ligusticum wallichii |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1438293A (en) * | 2002-12-26 | 2003-08-27 | 云南省农业科学院生物技术研究所 | Soil regulator for reatining water, oil and fertilizer and controlling pollution and preparing thereof |
| CN103703954A (en) * | 2012-10-05 | 2014-04-09 | 孙鑫 | Novel ligusticum-wallichii high-yield cultivation technique |
| CN104094769A (en) * | 2014-08-06 | 2014-10-15 | 四川新绿色药业科技发展股份有限公司 | Ligusticum chuanxiong hort frozen stem and preparation method thereof |
-
2015
- 2015-07-20 CN CN201510427406.2A patent/CN105165308A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1438293A (en) * | 2002-12-26 | 2003-08-27 | 云南省农业科学院生物技术研究所 | Soil regulator for reatining water, oil and fertilizer and controlling pollution and preparing thereof |
| CN103703954A (en) * | 2012-10-05 | 2014-04-09 | 孙鑫 | Novel ligusticum-wallichii high-yield cultivation technique |
| CN104094769A (en) * | 2014-08-06 | 2014-10-15 | 四川新绿色药业科技发展股份有限公司 | Ligusticum chuanxiong hort frozen stem and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 宁梓君等: "改良酸性土壤对川芎药用质量的影响", 《中国当代医药》 * |
| 杨关学等: "彭州市川芎的高产栽培技术", 《四川农业科技》 * |
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