CN105148029A - Method for preparing cordyceps sinensis oral liquid - Google Patents
Method for preparing cordyceps sinensis oral liquid Download PDFInfo
- Publication number
- CN105148029A CN105148029A CN201510402400.XA CN201510402400A CN105148029A CN 105148029 A CN105148029 A CN 105148029A CN 201510402400 A CN201510402400 A CN 201510402400A CN 105148029 A CN105148029 A CN 105148029A
- Authority
- CN
- China
- Prior art keywords
- liquid
- mixing
- preparation
- fermentation
- caterpillar fungus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a method for preparing cordyceps sinensis oral liquid, and belongs to the field of technologies for extracting active ingredients of plants. The method includes steps of preparing inclining strains of Chinese hirsutella sinensis; preparing liquid seeds; fermenting the inclining strains and the liquid seeds by the aid of fermentation tanks; adding medicinal materials into the fermentation tanks; carrying out superfine grinding; carrying out enzymatic hydrolysis; homogenizing the inclining strains, the liquid seeds and the medicinal materials; carrying out nanometer crushing; carrying out membrane treatment; further carrying out sterile filling to obtain the cordyceps sinensis oral liquid. The method has the advantages that the cordyceps sinensis oral liquid is a novel product with functions of nourishing the liver, tonifying the kidney, enhancing the immunity, improving sleep and the like; ingredients of cordyceps sinensis mycelia and fermentation liquid are integrated with one another in the product, nourishing healthcare functions of the diversified medicinal materials such as dendrobium officinale, American ginseng, barbary wolfberry fruits, licorice and jujube cooperate with one another, and the nutritious nourishing product with the comprehensive functions can be obtained by means of scientific compatibility and refining.
Description
Technical field
The invention belongs to active components of plants extractive technique field, particularly a kind of preparation method of Chinese caterpillar fungus oral liquid.
Background technology
Cordyceps has another name called Cordyceps, Cordyceps, Cordyceps sinensis etc., is the exclusive precious special product of China.According to categorizing system, Cordyceps Eumycota (Eumycota), Ascomycotina (Ascomycotina), gang pyrenomycetes (Pyrenomycrees), Clavicipitaceae (Clavicipitaceae), Cordyceps (Cordyceps), parasitizing for a kind of the entomogenous fungi formed in lepidopteran insects Hepialus armorieanus Oberthur larva, is also a kind of famous and precious medicinal fungi.
Cordyceps is as medicine resource, long-standing in China.For the understanding of Cordyceps, the B.C. Western Zhou Dynasty in 11st century can be traced back to the Qin Dynasty by China.The jade carving ornaments making pattern with Cordyceps are just found that there is from the historical relic be unearthed.Record XUECAN in the Compendium of Material Medica of 1578 year's harvest books, judging according to its life cycle, flavor efficacy, form, environment and the place of production, should be the ancient name of Cordyceps.In the essentials of Matea Medica of Qing Dynasty Wang Ang in 1694, also have the discussion about Cordyceps, this secretary is carried: " Cordyceps, Gan Ping protect lung kidney tonifying, hemostasis and phlegm, only phthisical cough.Institute product person is good for mansion, Jiading, Sichuan.Winter, shape was as old silkworm in soil, and hairiness is active, on being unearthed, connected body and all changed into grass to Xia Zemao ", this is the literature record the earliest of Cordyceps.Wu Yiluo (1757) first time in " Bencao Congxin " at large describes Cordyceps and medicinal.What the supplementary Amplifications of the Compendium of Materia Medica of ZHAO Xue-Min (1765) work returned it medicinal is " can control weakness ".In Traditional Chinese Medicine hundred taste, Cordyceps is considered to a kind of treasured of rare Chinese medicine and the China that makes a name with magical effect of " controlling weakness ", " equilibrium between yin and yang ", " protect lung kidney tonifying, replenishing vital essence and invigorating vital QI, specially mend the gate of vitality " just, enjoy great prestige overseas, equally celebrated for their achievements with Radix Ginseng, Cornu Cervi Pantotrichum, nominal is three large tonics of China.Cordyceps is the traditional strengthening by means of tonics medicine of China.Modern pharmacological research shows, Cordyceps is to adjustment that is immune and blood system, and the protection of kidney, liver, heart and injury and control, and defying age, anti-stress effect etc. all have good activity.Be used for the treatment of the systemic diseases such as cardiovascular disease, respiratory system disease, sexual dysfunction, renal failure, hepatic disease, tumor clinically, all achieve good curative effect.
At present, suitability for industrialized production China pilose spore, what mainly choose is mycelium, and fermentation liquid all goes out of use, and will carry out liquid waste processing so on the one hand, also result in the loss of Cordyceps precious resources in addition.Thus dig this kind of resource, not only can improve enterprise and value-added content of product, reduce costs, also can turn waste into wealth simultaneously, turn harm into good, be the road of living resources sustainable use, larger economic benefit and social benefit will be obtained.And laboratory pre-stage test finds, China pilose spore fermentation liquid has certain sedative-hypnotic effect, and this is just for the further research and development of fermentation liquid provide theoretical basis.Current also see Invention patent research Cordyceps fermented liquid concentrated after be developed to oral liquid, but due to composition single, pharmacologically active is inadequate, and range of application is restricted.
Along with the fierceness day by day of social competition, people's rhythm of life is constantly accelerated, and the sub-health population caused due to degradation factor under dietary habit, operating pressure, sleep quality also further expands to middle age by old people, or even population of adolescent.Therefore, national physical health issues is imperative.
Summary of the invention
The object of the invention is to overcome the shortcoming that exists in above-mentioned prior art with not enough, a kind of preparation method of Chinese caterpillar fungus oral liquid is provided.A kind of Chinese caterpillar fungus oral liquid product that Cordyceps fermented liquid becomes with medicinal ingredient hybrid process by the present invention; The product form being intended to solve Cordyceps class is dull, lacks the problem having liver nourishing kidney tonifying, improve the Cordyceps class oral liquid of the multiple health cares such as immunity.
Object of the present invention is achieved through the following technical solutions: a kind of preparation method of Chinese caterpillar fungus oral liquid, comprises the following steps:
(1) preparation of China pilose spore solid spawn;
(2) liquid seeds preparation;
(3) ferment tank;
(4) Herba Dendrobii, Radix Panacis Quinquefolii, Fructus Lycii, Radix Glycyrrhizae and Fructus Jujubae medical material micronizing;
(5) Cordyceps fermentation liquid and the mixing of medical material powder, homogenizing, enzymolysis;
(6) enzymolysis solution is through ceramic membrane filter, and reverse osmosis membrane is concentrated, sterile filling can obtain rufous Chinese caterpillar fungus oral liquid.
As a prioritization scheme of the present invention, the preparation of the China pilose spore solid spawn described in step (1), concrete steps are as follows: solid medium adopts Hepialus armorieanus Oberthur larva 1 ~ 3 part, Antheraea pernyi Geurin Meneville 4 ~ 8 parts, mulberry silkworm chrysalis 1 ~ 3 part, adopt historrhexis's instrument broken according to after mass ratio mixing raw material, homogenate, the feed liquid obtained, described feed liquid and fresh milk, water is the agar powder adding mass fraction 2% in the mixed material of 1:1:1 according to volume ratio, insert in large triangular flask, after 121 DEG C of sterilizing 15min ~ 20min, high speed disperser is adopted to disperse while hot with superclean bench, liquid subpackage after dispersion enters in 100ml wide-mouth triangular flask, every bottle of subpackage 40ml liquid, be cooled to room temperature, access China pilose spore solid seed, every bottle graft enters 2 ~ 3 pieces of 0.2m
2china pilose spore solid spawn block, 18 DEG C of light culture 10 ~ 20d, obtain China pilose spore solid spawn.
Described in step (1) is temperature more than 80 DEG C while hot.
As a prioritization scheme of the present invention, liquid seeds preparation described in step (2), concrete steps are as follows: liquid seed culture medium adopts Antheraea pernyi Geurin Meneville 4 ~ 8 parts, mulberry silkworm chrysalis 1 ~ 3 part, adopt historrhexis's instrument broken by after raw material mixing, homogenate, the feed liquid obtained, gained feed liquid and fresh milk, after water mixes according to the ratio that volume ratio is 1:1:5 ~ 10, 5 ~ 10g aminoacids complex is added in often liter of mixed liquor, 5 ~ 20g Fructus Hordei Germinatus powder, white sugar 10 ~ 30g, liquid subpackage after mixing enters in 1000ml wide-mouth triangular flask, every bottle of subpackage 400ml liquid, be cooled to room temperature, access China pilose spore solid seed, every bottle graft enters 2 ~ 3m
2china pilose spore solid spawn block, smash rear access triangular flask under aseptic condition to pieces, access 18 DEG C of light culture 7 ~ 10d, obtain liquid seeds.
As a prioritization scheme of the present invention, ferment tank described in step (3), concrete steps are as follows: liquid fermentation medium adopts Antheraea pernyi Geurin Meneville 4 ~ 8 parts, mulberry silkworm chrysalis 1 ~ 3 part, adopt historrhexis's instrument broken by after raw material mixing, homogenate, the feed liquid obtained, the feed liquid of gained and fresh milk, after water mixes according to the ratio that volume ratio is 1:1:5 ~ 10, 5 ~ 10g aminoacids complex is added in often liter of mixed liquor, 5 ~ 20g Fructus Hordei Germinatus powder, white sugar 10 ~ 30g, liquid subpackage after mixing enters airlift fermentor, take volume as 1000L airlift fermentor be example, fermentation tank liquid amount is 500 ~ 700L, tank pressure is 0.03 ~ 0.08Mpa, ventilation is volume ratio 1:0.9 (V/V), temperature 18 ~ 22 DEG C, lucifuge fermentation 7d, Cordyceps fermentation liquid can be obtained.
As a prioritization scheme of the present invention, Herba Dendrobii described in step (4), Radix Panacis Quinquefolii, Fructus Lycii, Radix Glycyrrhizae and Fructus Jujubae medical material micronizing, concrete steps are as follows: by Herba Dendrobii 1 ~ 2 part, Radix Panacis Quinquefolii 1 ~ 2 part, Fructus Lycii 1 ~ 2, Radix Glycyrrhizae 1 ~ 2,2 ~ 5 parts, Fructus Jujubae, further micronizing after coarse pulverization after mixing; Make material diameter≤10um; Obtain medical material powder.
As a prioritization scheme of the present invention, Cordyceps fermentation liquid described in step (5) and the mixing of medical material powder, homogenizing, enzymolysis, concrete steps are: mixed according to the mass ratio of 20 ~ 50:1 by the medical material powder obtained in the Cordyceps fermentation liquid obtained in step (3) and step (4), cellulase and amylase is added after adopting high-shear homogenizing machine homogenizing, the addition of its cellulase is 60 ~ 100U/g, diastatic addition is 100 ~ 300U/g, to material vibrating at constant temperature for enzymolysis 1 ~ 2h under the temperature conditions of 50 DEG C, after enzymolysis, material is warmed up to 80 DEG C and keeps 10min.
As a prioritization scheme of the present invention, enzymolysis solution described in step (6) is through ceramic membrane filter, reverse osmosis membrane is concentrated, sterile filling, concrete steps are: after the feed liquid after insulation in step (5) being adopted the ceramic membrane filter clarifying treatment of 0.45um, through reverse osmosis membrane simmer down to original volume 1/2, sterile filling, obtain the Chinese caterpillar fungus oral liquid of rufous.
In sum, the preparation method of Chinese caterpillar fungus oral liquid of the present invention, specifically comprises the following steps:
(1) preparation of China pilose spore solid spawn: solid medium adopts Hepialus armorieanus Oberthur larva 1 ~ 3 part, Antheraea pernyi Geurin Meneville 4 ~ 8 parts, mulberry silkworm chrysalis 1 ~ 3 part, adopt historrhexis's instrument broken according to after mass ratio mixing raw material, homogenate, the feed liquid obtained, described feed liquid and fresh milk, water is the agar powder adding mass fraction 2% in the mixed material of 1:1:1 according to volume ratio, insert in large triangular flask, after 121 DEG C of sterilizing 15min ~ 20min, taking advantage of more than 80 DEG C high temperature with superclean bench adopts high speed disperser to disperse, liquid subpackage after dispersion enters in 100ml wide-mouth triangular flask, every bottle of subpackage 40ml liquid, be cooled to room temperature, access China pilose spore solid seed, every bottle graft enters 2 ~ 3 pieces of 0.2m
2china pilose spore solid spawn block, 18 DEG C of light culture 10 ~ 20d, obtain China pilose spore solid spawn,
(2) liquid seeds preparation: liquid seed culture medium adopts Antheraea pernyi Geurin Meneville 4 ~ 8 parts, mulberry silkworm chrysalis 1 ~ 3 part, by adopting after raw material mixing, historrhexis's instrument is broken, homogenate, the feed liquid obtained, gained feed liquid and fresh milk, water are after the ratio mixing of 1:1:5 ~ 10 according to volume ratio, 5 ~ 10g aminoacids complex, 5 ~ 20g Fructus Hordei Germinatus powder is added in often liter of mixed liquor, white sugar 10 ~ 30g, liquid subpackage after mixing enters in 1000ml wide-mouth triangular flask, every bottle of subpackage 400ml liquid, be cooled to room temperature, access China pilose spore solid seed, every bottle graft enters 2 ~ 3m
2china pilose spore solid spawn block, smash rear access triangular flask under aseptic condition to pieces, access 18 DEG C of light culture 7 ~ 10d, obtain liquid seeds;
(3) ferment tank: liquid fermentation medium adopts Antheraea pernyi Geurin Meneville 4 ~ 8 parts, mulberry silkworm chrysalis 1 ~ 3 part, adopt historrhexis's instrument broken by after raw material mixing, homogenate, the feed liquid obtained, the feed liquid of gained and fresh milk, after water mixes according to the ratio that volume ratio is 1:1:5 ~ 10, 5 ~ 10g aminoacids complex is added in often liter of mixed liquor, 5 ~ 20g Fructus Hordei Germinatus powder, white sugar 10 ~ 30g, liquid subpackage after mixing enters airlift fermentor, take volume as 1000L airlift fermentor be example, fermentation tank liquid amount is 500 ~ 700L, tank pressure is 0.03 ~ 0.08Mpa, ventilation is volume ratio 1:0.9, temperature 18 ~ 22 DEG C, lucifuge fermentation 7d, Cordyceps fermentation liquid can be obtained,
(4) Herba Dendrobii, Radix Panacis Quinquefolii, Fructus Lycii, Radix Glycyrrhizae and Fructus Jujubae medical material micronizing: by Herba Dendrobii 1 ~ 2 part, Radix Panacis Quinquefolii 1 ~ 2 part, Fructus Lycii 1 ~ 2, Radix Glycyrrhizae 1 ~ 2,2 ~ 5 parts, Fructus Jujubae, further micronizing after coarse pulverization after mixing; Make material diameter≤10um; Obtain medical material powder;
(5) Cordyceps fermentation liquid and the mixing of medical material powder, homogenizing, enzymolysis: the medical material powder obtained in the Cordyceps fermentation liquid obtained in step (3) and step (4) is mixed according to the mass ratio of 20 ~ 50:1, cellulase and amylase is added after adopting high-shear homogenizing machine homogenizing, the addition of its cellulase is 60 ~ 100U/g, diastatic addition is 100 ~ 300U/g, to material vibrating at constant temperature for enzymolysis 1 ~ 2h under the temperature conditions of 50 DEG C, after enzymolysis, material is warmed up to 80 DEG C and keeps 10min;
(6) enzymolysis solution is through ceramic membrane filter, reverse osmosis membrane is concentrated, sterile filling: after the feed liquid after insulation in step (5) being adopted the ceramic membrane filter clarifying treatment of 0.45um, through reverse osmosis membrane simmer down to original volume 1/2, sterile filling, obtain the Chinese caterpillar fungus oral liquid of rufous.
A kind of Chinese caterpillar fungus oral liquid is obtained by above-mentioned preparation method.
The present invention has following advantage and effect relative to prior art:
Product prepared by the present invention is a kind of functional solid beverage with effects such as liver nourishing kidney tonifying, blood yiqi, raising immunity, effectively remains the composition of Cordyceps and medical material in this product.Life cycle of the product of the present invention is short, process of producing product Raw is through kinds of processes such as liquid fermentation, enzymolysis, membrane filtrations, fully retaining medical material all the components in product, no waste discharge in production process, is that a kind of product output rate is high, production technology simple, the production method of environmental protection.In addition, Chinese caterpillar fungus oral liquid of the present invention, be especially applicable to the sub-health population that liver renal function is bad, have a delicate constitution etc., this product has widened the application of the products such as Cordyceps, Herba Dendrobii, Radix Panacis Quinquefolii.
Accompanying drawing explanation
Fig. 1 is the flow chart of preparation method of the present invention.
Detailed description of the invention
Below in conjunction with embodiment and accompanying drawing, the present invention is described in further detail, but embodiments of the present invention are not limited thereto.
Embodiment 1
A preparation method for Chinese caterpillar fungus oral liquid, as shown in Figure 1, comprises the following steps:
(1) preparation of China pilose spore solid spawn: solid medium adopts Hepialus armorieanus Oberthur larva 1 ~ 3 part, Antheraea pernyi Geurin Meneville 4 ~ 8 parts, mulberry silkworm chrysalis 1 ~ 3 part, adopt historrhexis's instrument broken according to after mass ratio mixing raw material, homogenate, the feed liquid obtained, described feed liquid and fresh milk, water is the agar powder adding mass fraction 2% in the mixed material of 1:1:1 according to volume ratio, insert in large triangular flask, after 121 DEG C of sterilizing 15min ~ 20min, with superclean bench while hot (more than 80 DEG C) adopt high speed disperser to disperse, liquid subpackage after dispersion enters in 100ml wide-mouth triangular flask, every bottle of subpackage 40ml liquid, be cooled to room temperature, access China pilose spore solid seed, every bottle graft enters 2 ~ 3 pieces of 0.2m
2china pilose spore solid spawn block, 18 DEG C of light culture 10 ~ 20d, obtain China pilose spore solid spawn,
(2) liquid seeds preparation: liquid seed culture medium adopts Antheraea pernyi Geurin Meneville 4 ~ 8 parts, mulberry silkworm chrysalis 1 ~ 3 part, by adopting after raw material mixing, historrhexis's instrument is broken, homogenate, the feed liquid obtained, gained feed liquid and fresh milk, water are after the ratio mixing of 1:1:5 ~ 10 according to volume ratio, 5 ~ 10g aminoacids complex, 5 ~ 20g Fructus Hordei Germinatus powder is added in often liter of mixed liquor, white sugar 10 ~ 30g, liquid subpackage after mixing enters in 1000ml wide-mouth triangular flask, every bottle of subpackage 400ml liquid, be cooled to room temperature, access China pilose spore solid seed, every bottle graft enters 2 ~ 3m
2china pilose spore solid spawn block, smash rear access triangular flask under aseptic condition to pieces, access 18 DEG C of light culture 7 ~ 10d, obtain liquid seeds;
(3) ferment tank: liquid fermentation medium adopts Antheraea pernyi Geurin Meneville 4 ~ 8 parts, mulberry silkworm chrysalis 1 ~ 3 part, adopt historrhexis's instrument broken by after raw material mixing, homogenate, the feed liquid obtained, the feed liquid of gained and fresh milk, after water mixes according to the ratio that volume ratio is 1:1:5 ~ 10, 5 ~ 10g aminoacids complex is added in often liter of mixed liquor, 5 ~ 20g Fructus Hordei Germinatus powder, white sugar 10 ~ 30g, liquid subpackage after mixing enters airlift fermentor, take volume as 1000L airlift fermentor be example, fermentation tank liquid amount is 500 ~ 700L, tank pressure is 0.03 ~ 0.08Mpa, ventilation is volume ratio 1:0.9 (V/V), temperature 18 ~ 22 DEG C, lucifuge fermentation 7d, Cordyceps fermentation liquid can be obtained,
(4) Herba Dendrobii, Radix Panacis Quinquefolii, Fructus Lycii, Radix Glycyrrhizae and Fructus Jujubae medical material micronizing: by Herba Dendrobii 1 ~ 2 part, Radix Panacis Quinquefolii 1 ~ 2 part, Fructus Lycii 1 ~ 2, Radix Glycyrrhizae 1 ~ 2,2 ~ 5 parts, Fructus Jujubae, further micronizing after coarse pulverization after mixing; Make material diameter≤10um; Obtain medical material powder;
(5) Cordyceps fermentation liquid and the mixing of medical material powder, homogenizing, enzymolysis: the medical material powder obtained in the Cordyceps fermentation liquid obtained in step (3) and step (4) is mixed according to the mass ratio of 20 ~ 50:1,
Cellulase and amylase is added after adopting high-shear homogenizing machine homogenizing, the addition of its cellulase is 60 ~ 100U/g, diastatic addition is 100 ~ 300U/g, to material vibrating at constant temperature for enzymolysis 1 ~ 2h under the temperature conditions of 50 DEG C, after enzymolysis, material is warmed up to 80 DEG C and keeps 10min;
(6) enzymolysis solution is through ceramic membrane filter, reverse osmosis membrane is concentrated, sterile filling: after the feed liquid after insulation in step (5) being adopted the ceramic membrane filter clarifying treatment of 0.45um, through reverse osmosis membrane simmer down to original volume 1/2, sterile filling, obtain the Chinese caterpillar fungus oral liquid of rufous.
Product prepared by the present invention is a kind of functional solid beverage with effects such as liver nourishing kidney tonifying, blood yiqi, raising immunity, effectively remains the composition of Cordyceps and medical material in this product.Life cycle of the product of the present invention is short, process of producing product Raw is through kinds of processes such as liquid fermentation, enzymolysis, membrane filtrations, fully retaining medical material all the components in product, no waste discharge in production process, is that a kind of product output rate is high, production technology simple, the production method of environmental protection.In addition, Chinese caterpillar fungus oral liquid of the present invention, be especially applicable to the sub-health population that liver renal function is bad, have a delicate constitution etc., this product has widened the application of the products such as Cordyceps, Herba Dendrobii, Radix Panacis Quinquefolii.
Above-described embodiment is the present invention's preferably embodiment; but embodiments of the present invention are not restricted to the described embodiments; change, the modification done under other any does not deviate from spirit of the present invention and principle, substitute, combine, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (10)
1. a preparation method for Chinese caterpillar fungus oral liquid, is characterized in that: comprise the following steps:
(1) preparation of China pilose spore solid spawn;
(2) liquid seeds preparation;
(3) ferment tank;
(4) Herba Dendrobii, Radix Panacis Quinquefolii, Fructus Lycii, Radix Glycyrrhizae and Fructus Jujubae medical material micronizing;
(5) Cordyceps fermentation liquid and the mixing of medical material powder, homogenizing, enzymolysis;
(6) enzymolysis solution is through ceramic membrane filter, and reverse osmosis membrane is concentrated, sterile filling can obtain rufous Chinese caterpillar fungus oral liquid.
2. the preparation method of Chinese caterpillar fungus oral liquid according to claim 1, it is characterized in that: the preparation of the China pilose spore solid spawn described in step (1), concrete steps are as follows: solid medium adopts Hepialus armorieanus Oberthur larva 1 ~ 3 part, Antheraea pernyi Geurin Meneville 4 ~ 8 parts, mulberry silkworm chrysalis 1 ~ 3 part, adopt historrhexis's instrument broken according to after mass ratio mixing raw material, homogenate, the feed liquid obtained, described feed liquid and fresh milk, water is the agar powder adding mass fraction 2% in the mixed material of 1:1:1 according to volume ratio, insert in large triangular flask, after 121 DEG C of sterilizing 15min ~ 20min, high speed disperser is adopted to disperse while hot with superclean bench, liquid subpackage after dispersion enters in 100ml wide-mouth triangular flask, every bottle of subpackage 40ml liquid, be cooled to room temperature, access China pilose spore solid seed, every bottle graft enters 2 ~ 3 pieces of 0.2m
2china pilose spore solid spawn block, 18 DEG C of light culture 10 ~ 20d, obtain China pilose spore solid spawn.
3. the preparation method of Chinese caterpillar fungus oral liquid according to claim 2, is characterized in that: described in step (1) is temperature more than 80 DEG C while hot.
4. the preparation method of Chinese caterpillar fungus oral liquid according to claim 3, it is characterized in that: the liquid seeds preparation described in step (2), concrete steps are as follows: liquid seed culture medium adopts Antheraea pernyi Geurin Meneville 4 ~ 8 parts, mulberry silkworm chrysalis 1 ~ 3 part, adopt historrhexis's instrument broken by after raw material mixing, homogenate, the feed liquid obtained, gained feed liquid and fresh milk, after water mixes according to the ratio that volume ratio is 1:1:5 ~ 10, 5 ~ 10g aminoacids complex is added in often liter of mixed liquor, 5 ~ 20g Fructus Hordei Germinatus powder, white sugar 10 ~ 30g, liquid subpackage after mixing enters in 1000ml wide-mouth triangular flask, every bottle of subpackage 400ml liquid, be cooled to room temperature, access China pilose spore solid seed, every bottle graft enters 2 ~ 3m
2china pilose spore solid spawn block, smash rear access triangular flask under aseptic condition to pieces, access 18 DEG C of light culture 7 ~ 10d, obtain liquid seeds.
5. the preparation method of Chinese caterpillar fungus oral liquid according to claim 4, it is characterized in that: the ferment tank described in step (3), concrete steps are as follows: liquid fermentation medium adopts Antheraea pernyi Geurin Meneville 4 ~ 8 parts, mulberry silkworm chrysalis 1 ~ 3 part, adopt historrhexis's instrument broken by after raw material mixing, homogenate, the feed liquid obtained, the feed liquid of gained and fresh milk, after water mixes according to the ratio that volume ratio is 1:1:5 ~ 10, 5 ~ 10g aminoacids complex is added in often liter of mixed liquor, 5 ~ 20g Fructus Hordei Germinatus powder, white sugar 10 ~ 30g, liquid subpackage after mixing enters airlift fermentor, take volume as 1000L airlift fermentor be example, fermentation tank liquid amount is 500 ~ 700L, tank pressure is 0.03 ~ 0.08Mpa, ventilation is volume ratio 1:0.9 (V/V), temperature 18 ~ 22 DEG C, lucifuge fermentation 7d, Cordyceps fermentation liquid can be obtained.
6. the preparation method of Chinese caterpillar fungus oral liquid according to claim 5, it is characterized in that: the Herba Dendrobii described in step (4), Radix Panacis Quinquefolii, Fructus Lycii, Radix Glycyrrhizae and Fructus Jujubae medical material micronizing, concrete steps are as follows: by Herba Dendrobii 1 ~ 2 part, Radix Panacis Quinquefolii 1 ~ 2 part, Fructus Lycii 1 ~ 2, Radix Glycyrrhizae 1 ~ 2,2 ~ 5 parts, Fructus Jujubae, further micronizing after coarse pulverization after mixing; Make material diameter≤10um; Obtain medical material powder.
7. the preparation method of Chinese caterpillar fungus oral liquid according to claim 6, it is characterized in that: the Cordyceps fermentation liquid described in step (5) and the mixing of medical material powder, homogenizing, enzymolysis, concrete steps are: mixed according to the mass ratio of 20 ~ 50:1 by the medical material powder obtained in the Cordyceps fermentation liquid obtained in step (3) and step (4), cellulase and amylase is added after adopting high-shear homogenizing machine homogenizing, the addition of its cellulase is 60 ~ 100U/g, diastatic addition is 100 ~ 300U/g, to material vibrating at constant temperature for enzymolysis 1 ~ 2h under the temperature conditions of 50 DEG C, after enzymolysis, material is warmed up to 80 DEG C and keeps 10min.
8. the preparation method of Chinese caterpillar fungus oral liquid according to claim 7, it is characterized in that: the enzymolysis solution described in step (6) is through ceramic membrane filter, reverse osmosis membrane is concentrated, sterile filling, concrete steps are: after the feed liquid after insulation in step (5) being adopted the ceramic membrane filter clarifying treatment of 0.45um, through reverse osmosis membrane simmer down to original volume 1/2, sterile filling, obtain the Chinese caterpillar fungus oral liquid of rufous.
9. the preparation method of Chinese caterpillar fungus oral liquid according to claim 1, is characterized in that: specifically comprise the following steps:
(1) preparation of China pilose spore solid spawn: solid medium adopts Hepialus armorieanus Oberthur larva 1 ~ 3 part, Antheraea pernyi Geurin Meneville 4 ~ 8 parts, mulberry silkworm chrysalis 1 ~ 3 part, adopt historrhexis's instrument broken according to after mass ratio mixing raw material, homogenate, the feed liquid obtained, described feed liquid and fresh milk, water is the agar powder adding mass fraction 2% in the mixed material of 1:1:1 according to volume ratio, insert in large triangular flask, after 121 DEG C of sterilizing 15min ~ 20min, taking advantage of more than 80 DEG C high temperature with superclean bench adopts high speed disperser to disperse, liquid subpackage after dispersion enters in 100ml wide-mouth triangular flask, every bottle of subpackage 40ml liquid, be cooled to room temperature, access China pilose spore solid seed, every bottle graft enters 2 ~ 3 pieces of 0.2m
2china pilose spore solid spawn block, 18 DEG C of light culture 10 ~ 20d, obtain China pilose spore solid spawn,
(2) liquid seeds preparation: liquid seed culture medium adopts Antheraea pernyi Geurin Meneville 4 ~ 8 parts, mulberry silkworm chrysalis 1 ~ 3 part, by adopting after raw material mixing, historrhexis's instrument is broken, homogenate, the feed liquid obtained, gained feed liquid and fresh milk, water are after the ratio mixing of 1:1:5 ~ 10 according to volume ratio, 5 ~ 10g aminoacids complex, 5 ~ 20g Fructus Hordei Germinatus powder is added in often liter of mixed liquor, white sugar 10 ~ 30g, liquid subpackage after mixing enters in 1000ml wide-mouth triangular flask, every bottle of subpackage 400ml liquid, be cooled to room temperature, access China pilose spore solid seed, every bottle graft enters 2 ~ 3m
2china pilose spore solid spawn block, smash rear access triangular flask under aseptic condition to pieces, access 18 DEG C of light culture 7 ~ 10d, obtain liquid seeds;
(3) ferment tank: liquid fermentation medium adopts Antheraea pernyi Geurin Meneville 4 ~ 8 parts, mulberry silkworm chrysalis 1 ~ 3 part, adopt historrhexis's instrument broken by after raw material mixing, homogenate, the feed liquid obtained, the feed liquid of gained and fresh milk, after water mixes according to the ratio that volume ratio is 1:1:5 ~ 10, 5 ~ 10g aminoacids complex is added in often liter of mixed liquor, 5 ~ 20g Fructus Hordei Germinatus powder, white sugar 10 ~ 30g, liquid subpackage after mixing enters airlift fermentor, take volume as 1000L airlift fermentor be example, fermentation tank liquid amount is 500 ~ 700L, tank pressure is 0.03 ~ 0.08Mpa, ventilation is volume ratio 1:0.9, temperature 18 ~ 22 DEG C, lucifuge fermentation 7d, Cordyceps fermentation liquid can be obtained,
(4) Herba Dendrobii, Radix Panacis Quinquefolii, Fructus Lycii, Radix Glycyrrhizae and Fructus Jujubae medical material micronizing: by Herba Dendrobii 1 ~ 2 part, Radix Panacis Quinquefolii 1 ~ 2 part, Fructus Lycii 1 ~ 2, Radix Glycyrrhizae 1 ~ 2,2 ~ 5 parts, Fructus Jujubae, further micronizing after coarse pulverization after mixing; Make material diameter≤10um; Obtain medical material powder;
(5) Cordyceps fermentation liquid and the mixing of medical material powder, homogenizing, enzymolysis: the medical material powder obtained in the Cordyceps fermentation liquid obtained in step (3) and step (4) is mixed according to the mass ratio of 20 ~ 50:1, cellulase and amylase is added after adopting high-shear homogenizing machine homogenizing, the addition of its cellulase is 60 ~ 100U/g, diastatic addition is 100 ~ 300U/g, to material vibrating at constant temperature for enzymolysis 1 ~ 2h under the temperature conditions of 50 DEG C, after enzymolysis, material is warmed up to 80 DEG C and keeps 10min;
(6) enzymolysis solution is through ceramic membrane filter, reverse osmosis membrane is concentrated, sterile filling: after the feed liquid after insulation in step (5) being adopted the ceramic membrane filter clarifying treatment of 0.45um, through reverse osmosis membrane simmer down to original volume 1/2, sterile filling, obtain the Chinese caterpillar fungus oral liquid of rufous.
10. a Chinese caterpillar fungus oral liquid is obtained by the preparation method described in any one of claim 1 ~ 9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510402400.XA CN105148029A (en) | 2015-07-08 | 2015-07-08 | Method for preparing cordyceps sinensis oral liquid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510402400.XA CN105148029A (en) | 2015-07-08 | 2015-07-08 | Method for preparing cordyceps sinensis oral liquid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105148029A true CN105148029A (en) | 2015-12-16 |
Family
ID=54789293
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510402400.XA Pending CN105148029A (en) | 2015-07-08 | 2015-07-08 | Method for preparing cordyceps sinensis oral liquid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105148029A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106244390A (en) * | 2016-08-05 | 2016-12-21 | 锬酃藏虫草生物科技(深圳)有限公司 | A kind of Herba Dendrobii Cordyceps fermentation original plasm wine and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1372002A (en) * | 2001-02-26 | 2002-10-02 | 孙悦迎 | Method for producing cordycepin powder and active immne cordyceps sinensis polysaccharide frozen dried powder and products thereof |
| CN1895294A (en) * | 2006-05-22 | 2007-01-17 | 安徽农业大学 | Chinese pilose spore concentrated oral liquid |
| CN102349975A (en) * | 2011-10-11 | 2012-02-15 | 杭州天目山药业股份有限公司 | Health care product formula of dendrobium officinale, composition as well as preparation method and application of composition |
| CN102550283A (en) * | 2010-12-30 | 2012-07-11 | 杨发国 | Technology for artificially breeding cordyceps sinensis by substituting silkworm pupae for hepialus armoricanus |
| CN103099966A (en) * | 2013-01-15 | 2013-05-15 | 延安大学附属医院 | Stomach-invigorating and spleen-benefiting potion |
-
2015
- 2015-07-08 CN CN201510402400.XA patent/CN105148029A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1372002A (en) * | 2001-02-26 | 2002-10-02 | 孙悦迎 | Method for producing cordycepin powder and active immne cordyceps sinensis polysaccharide frozen dried powder and products thereof |
| CN1895294A (en) * | 2006-05-22 | 2007-01-17 | 安徽农业大学 | Chinese pilose spore concentrated oral liquid |
| CN102550283A (en) * | 2010-12-30 | 2012-07-11 | 杨发国 | Technology for artificially breeding cordyceps sinensis by substituting silkworm pupae for hepialus armoricanus |
| CN102349975A (en) * | 2011-10-11 | 2012-02-15 | 杭州天目山药业股份有限公司 | Health care product formula of dendrobium officinale, composition as well as preparation method and application of composition |
| CN103099966A (en) * | 2013-01-15 | 2013-05-15 | 延安大学附属医院 | Stomach-invigorating and spleen-benefiting potion |
Non-Patent Citations (1)
| Title |
|---|
| 秦鹏等: "冬虫夏草无性型发酵技术研究进展", 《中国酿造》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106244390A (en) * | 2016-08-05 | 2016-12-21 | 锬酃藏虫草生物科技(深圳)有限公司 | A kind of Herba Dendrobii Cordyceps fermentation original plasm wine and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106244372B (en) | Enzyme-fermented gastrodia elata health-preserving beverage for improving sleep and preparation process and application thereof | |
| CN101099513B (en) | Cordyceps sinensis dried milk slices and producing method thereof | |
| CN104856026A (en) | Preparation method of cordyceps and arctium lappa enzyme | |
| CN105878764B (en) | A kind of Cordyceps militaris electuary and preparation method thereof | |
| CN105193905A (en) | Traditional Chinese medicine processing technology of ganoderma-astragalus membranaceus bidirectional fermentation | |
| CN105030846A (en) | Preparation method of cordyceps militaris superfine powder | |
| CN101967440B (en) | Dry or semi-dry hawthorn wine and brewing method thereof | |
| CN106281849A (en) | A kind of brewing method of low methanol green tea wine | |
| CN103525636A (en) | Formula of ganoderma lucidum wine and preparation method of ganoderma lucidum wine | |
| CN106367284A (en) | Liquid submerged fermentation technique of Chinese wolfberry/licorice root/apple health-care vinegar | |
| CN1393262A (en) | Process for preparing nutritive health-care oral liquid | |
| CN104711143A (en) | Dioscorea polystachya wine preparation method | |
| CN106244390A (en) | A kind of Herba Dendrobii Cordyceps fermentation original plasm wine and preparation method thereof | |
| CN106387888A (en) | Composition used for producing functional foods, health-care products and medicines | |
| CN105148029A (en) | Method for preparing cordyceps sinensis oral liquid | |
| CN108865584A (en) | A kind of brewing method of sweet wine | |
| CN104672345A (en) | Method for preparing crude polysaccharide from steindachner soft-shelled turtle carapace | |
| CN110025522A (en) | A kind of hair follicle stem cells wake up factor and preparation method thereof | |
| CN103911294A (en) | Liquid fermentation method of ginseng-radix astragali-lucid ganoderma medical mycoplasm | |
| CN108841515A (en) | A kind of production method of koji | |
| CN103497873B (en) | A kind of active Gastrodia elata original plasm wine and preparation method thereof | |
| CN102771769B (en) | Ganoderma sinensis health product and preparation method thereof | |
| CN107080016A (en) | A kind of relieving mental strain and helping sleep tea bag and preparation method thereof | |
| KR20030074558A (en) | Method of manufacturing functional distilled liquor, coarse liquor, rice wine and medicinal wines with aqueous solution of selenium, ginkgo biloba leaves, pine needle amino acid, ginseng saponin | |
| CN104415185A (en) | Shengjing oral solution and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20151216 |