CN110025522A - A kind of hair follicle stem cells wake up factor and preparation method thereof - Google Patents
A kind of hair follicle stem cells wake up factor and preparation method thereof Download PDFInfo
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Abstract
The present invention relates to hair care field, a kind of hair follicle stem cells are provided and wake up factor and preparation method thereof, for solving the problems, such as that alopeciaing therapeutic effect is poor.A kind of hair follicle stem cells provided by the invention wake up the factor, comprising: 5 ~ 10 mass parts of cordyceps extracting liquid, 9 ~ 12 mass parts of lucidum extracting liquid, 11 ~ 15 mass parts of tuber of multiflower knotweed extracting solution, 7 ~ 9 mass parts of Ginseng extract, 0.1 ~ 0.5 mass parts of chitosan.By Chinese traditional herbs come hair follicle stimulating stem cell, and then hair follicle stem cells differentiation is activated, improves the vigor of natural on-off cycles of hair growth, promote the growth of hair.
Description
Technical field
The present invention relates to hair care fields, and in particular to a kind of hair follicle stem cells wake up factor and preparation method thereof.
Background technique
The related project such as the growth mechanism of hair and its maintenance, including alopecia-stopping, hair tonic, black hair is for a long time related subject
It is of interest.
Currently, product in the market is broadly divided into two major classes: one kind is, using Chinese herbal medicine as major ingredient, another kind of is with synthetic drug
Object, such as the germinal preparation that minoxidil (Minoxidil) is major ingredient.
It is right although being only limited to the use of generally common prescription using the germinal preparation of Chinese herbal medicine using wide in China
Further the research with regard to hair tonic, black hair mechanism in relation to hair and exploration and deeply excavated as technique direction Chinese herbal medicine essence
China jumps onto hair care hair products higher level to be still weak.
Summary of the invention
Present invention solves the technical problem that being the problem of alopeciaing therapeutic effect difference, a kind of hair follicle stem cells wake-up factor is provided
And preparation method thereof.
In order to solve the above technical problem, the present invention provides technical solution are as follows:
A kind of hair follicle stem cells wake-up factor, comprising: 5 ~ 10 mass parts of cordyceps extracting liquid, 9 ~ 12 mass of lucidum extracting liquid
Part, 11 ~ 15 mass parts of tuber of multiflower knotweed extracting solution, 7 ~ 9 mass parts of Ginseng extract, 0.1 ~ 0.5 mass parts of chitosan.
By compounding to a variety of Chinese traditional herbs, alopecia on number of people skin or baldness can be waken up to a certain extent
Hair follicle stem cells in region, and then it is promoted to be proliferated and break up, promote natural on-off cycles of hair growth.
By Chinese traditional herbs come hair follicle stimulating stem cell, and then hair follicle stem cells differentiation is activated, improves natural on-off cycles of hair growth
Vigor, promote the growth of hair.
Preferably, comprising: 7 ~ 10 mass parts of cordyceps extracting liquid, 10 ~ 12 mass parts of lucidum extracting liquid, tuber of multiflower knotweed extracting solution
12 ~ 15 mass parts, 8 ~ 9 mass parts of Ginseng extract, 0.2 ~ 0.5 mass parts of chitosan.
The dosage of Chinese medicine is optimized, the probability that hair follicle stem cells are activated can be improved, it is further to promote
The growth of hair.
Preferably, comprising: 7 mass parts of cordyceps extracting liquid, 10 mass parts of lucidum extracting liquid, 12 mass of tuber of multiflower knotweed extracting solution
Part, 8 mass parts of Ginseng extract, 0.2 mass parts of chitosan.
Preferably, the Ginseng extract the preparation method comprises the following steps:
S41. cellulase, pectase are add to deionized water, with the ginseng mixing thinly sliced, react 1h at 45 DEG C,
Obtain enzyme-extracting solution；
S42. 95% ethyl alcohol, 55 DEG C of reflux 3h is added for the first time into enzyme-extracting solution, filtering obtains first extract and residue, residual
The ethyl alcohol of second of addition 95% of slag extracts, and 55 DEG C of reflux 2h merge with first extract, obtain alcohol extract, the first time second
Alcohol additional amount: second of amount of alcohol added=1:4；
S43. alcohol extract is concentrated under reduced pressure at 50 DEG C into medicinal extract shape, ethyl alcohol is recycled in concentration process, obtained medicinal extract vacuum is dry
It is dry both to obtain ginseng extract；By ginseng extract to be added in ethyl alcohol, Ginseng extract, the ginseng extract: second are obtained
Alcohol=2 ~ 5:100.
The extract in ginseng is extracted in a manner of enzymolysis and extraction, can retain the effective substance in ginseng as far as possible
Matter promotes the effect of natural on-off cycles of hair growth more obvious after the same tuber of multiflower knotweed, ganoderma lucidum etc. compound.
Preferably, the cellulase, pectase, deionized water, ginseng mass ratio be 5 × 10-4:10-3:1:4。
Preferably, the ethyl alcohol is 1:0.4 with the mass ratio of enzyme-extracting solution.
Active principle in enzyme-extracting solution is extracted again by ethyl alcohol, available sufficient correct hair growth result
More obvious extract.
Preferably, the chitosan be modification of chitosan, the modification of chitosan the preparation method comprises the following steps:
S71. 10 ~ 100 mass parts of chitosan are taken, 0.5 ~ 2 mass parts of nano silver, 2 ~ 10 mass parts of human-like collagen, glycerol 10 ~
50 mass parts, 1 ~ 2 mass parts of carbomer, 0.6 ~ 1.5 mass parts of Geniposide add chitosan into deionized water, and stirring is equal
It is even, nano silver is added and glycerol, ultrasonic disperse obtain mixed liquor；
S72. Geniposide is added in mixed liquor, 36 ~ 48h is reacted at 35 ~ 40 DEG C, adds carbomer, adjust solution to
pH=7；
S73. human-like collagen is added, after mixing evenly, obtained solid substance score it is poly- must to be both modified shell by freeze-drying
Sugar.
Modified chitosan is compounded with Chinese herbal medicine extract, be can be improved and is waken up the time that the factor retains on scalp, into
And the lasting hair follicle stem cells on scalp are provided rich in the environment for waking up the factor.
Preferably, the nano silver is modified Nano silver, the method for modifying of the nano silver are as follows:
S81. 1 ~ 2 mass parts of nano silver are taken, are added in the polyethylene glycol of 4 ~ 6 mass parts, ultrasonic disperse, nano silver dispersion is obtained
Liquid；
S82. 1.5 ~ 3 mass parts of nano-titanium dioxide are taken, are added in the deionized water of 10 ~ 15 mass parts, ultrasonic disperse is received
Rice titanium oxide dispersion；
S83. after nano silver dispersion being mixed with nano titanium oxide dispersion, 1 ~ 5h of ultrasonic disperse, vacuum drying is changed
Property nano silver.
Chitosan is modified using modified nano silver, can be further improved and wake up factor wake-up hair follicle stem cells
Quantity, and then improve hair increment.
Preferably, the nano-titanium dioxide is modified nano-titanium dioxide, the modification of the modified nano-titanium dioxide
Method are as follows: tetraethyl titanate and nickel acetylacetonate are added in benzene, obtain precursor solution, the concentration of the tetraethyl titanate is
0.5 ~ 0.8mol/L, the concentration of the nickel acetylacetonate are 0.01 ~ 0.3mol/L；By precursor solution with 3 ~ 10ml/min into
Material speed assists shearing atomization burner to form fine atomized drop through external oxygen, and microlayer model is in H2/O2The auxiliary of diffusion flame
Under be converted into nanometer powder, the shear pressure at the shearing atomization mouthpiece is 0.2 ~ 0.3MPa, H in the diffusion flame2's
Flow is 150 ~ 300L/h, O2Flow be 700 ~ 1200L/h；Nanometer powder is calcined into 3 ~ 6h, calcining temperature in air atmosphere
Degree is 300 ~ 500 DEG C, obtains modifying titanium dioxide after cooling.
The effective substance waken up in the factor can be further improved using modified nano-titanium dioxide modified nano silver
Matter rests on scalp, and then provides the approach effectively waken up for scalp for a long time.
Preferably, cordyceps extracting liquid, lucidum extracting liquid, tuber of multiflower knotweed extracting solution, Ginseng extract, chitosan are taken, sufficiently
It after mixing, stirs evenly, 37 DEG C of 1 ~ 2h of water-bath.
The extracting solution of Chinese herbal medicine is simply mixed, the effect after not only having remained combined Chinese herb with chitosan, but also can grown
Effect is that scalp provides the environment of hair stem cells Proliferation, Differentiation.
Compared with prior art, the device have the advantages that are as follows: by Chinese traditional herbs come hair follicle stimulating stem cell,
And then hair follicle stem cells differentiation is activated, the vigor of natural on-off cycles of hair growth is improved, the growth of hair is promoted；By the extracting solution of Chinese herbal medicine
It is simply mixed with chitosan, the effect after not only having remained combined Chinese herb, but also long-acting can provide hair stem cells for scalp
The environment of Proliferation, Differentiation；It can further be improved and be waken up in the factor using modified nano-titanium dioxide modified nano silver
Active principle rests on scalp, and then provides the approach effectively waken up for scalp for a long time.
Specific embodiment
Following implementation column is to further explanation of the invention, is not limitation of the present invention.
Embodiment 1
A kind of hair follicle stem cells wake-up factor, comprising: 7 mass parts of cordyceps extracting liquid, 10 mass parts of lucidum extracting liquid, the tuber of multiflower knotweed
12 mass parts of extracting solution, 8 mass parts of Ginseng extract, 0.2 mass parts of chitosan.
The Ginseng extract the preparation method comprises the following steps:
S41. cellulase, pectase are add to deionized water, with the ginseng mixing thinly sliced, react 1h at 45 DEG C,
Obtain enzyme-extracting solution；
S42. 95% ethyl alcohol, 55 DEG C of reflux 3h is added for the first time into enzyme-extracting solution, filtering obtains first extract and residue, residual
The ethyl alcohol of second of addition 95% of slag extracts, and 55 DEG C of reflux 2h merge with first extract, obtain alcohol extract, the first time second
Alcohol additional amount: second of amount of alcohol added=1:4；
S43. alcohol extract is concentrated under reduced pressure at 50 DEG C into medicinal extract shape, ethyl alcohol is recycled in concentration process, obtained medicinal extract vacuum is dry
It is dry both to obtain ginseng extract；By ginseng extract to be added in ethyl alcohol, Ginseng extract, the ginseng extract: second are obtained
Alcohol=3:100.
The cellulase, pectase, deionized water, ginseng mass ratio be 5 × 10-4:10-3:1:4。
The ethyl alcohol is 1:0.4 with the mass ratio of enzyme-extracting solution.
The chitosan be modification of chitosan, the modification of chitosan the preparation method comprises the following steps:
S71. 20 mass parts of chitosan, 1 mass parts of nano silver, 2 ~ 10 mass parts of human-like collagen, 25 mass parts of glycerol, card are taken
1.5 mass parts of wave nurse, 1 mass parts of Geniposide, add chitosan into deionized water, stir evenly, and nano silver and sweet is added
Oil, ultrasonic disperse obtain mixed liquor；
S72. Geniposide is added in mixed liquor, reacts 40h at 37 DEG C, add carbomer, adjust solution to pH=7；
S73. human-like collagen is added, after mixing evenly, obtained solid substance score it is poly- must to be both modified shell by freeze-drying
Sugar.
The nano silver is modified Nano silver, the method for modifying of the nano silver are as follows:
S81. 1.5 mass parts of nano silver are taken, are added in the polyethylene glycol of 5 mass parts, ultrasonic disperse, nano silver dispersion is obtained
Liquid；
S82. 2 mass parts of nano-titanium dioxide are taken, are added in the deionized water of 12 mass parts, ultrasonic disperse obtains nano-silica
Change titanium dispersion liquid；
S83. after nano silver dispersion being mixed with nano titanium oxide dispersion, ultrasonic disperse 3h, vacuum drying is modified
Nano silver.
The nano-titanium dioxide is modified nano-titanium dioxide, the method for modifying of the modified nano-titanium dioxide are as follows:
Tetraethyl titanate and nickel acetylacetonate are added in benzene, precursor solution is obtained, the concentration of the tetraethyl titanate is 0.6mol/L,
The concentration of the nickel acetylacetonate is 0.1mol/L；Precursor solution is assisted with the charging rate of 5ml/min through external oxygen
Shearing atomization burner forms fine atomized drop, and microlayer model is in H2/O2Nanometer powder, institute are converted under the auxiliary of diffusion flame
Stating the shear pressure at shearing atomization mouthpiece is 0.25MPa, H in the diffusion flame2Flow be 200L/h, O2Flow be
1000L/h；Nanometer powder is calcined into 5h in air atmosphere, calcination temperature is 400 DEG C, obtains modifying titanium dioxide after cooling.
By compounding to a variety of Chinese traditional herbs, alopecia on number of people skin or baldness can be waken up to a certain extent
Hair follicle stem cells in region, and then it is promoted to be proliferated and break up, promote natural on-off cycles of hair growth.
By Chinese traditional herbs come hair follicle stimulating stem cell, and then hair follicle stem cells differentiation is activated, improves natural on-off cycles of hair growth
Vigor, promote the growth of hair.
The dosage of Chinese medicine is optimized, the probability that hair follicle stem cells are activated can be improved, it is further to promote
The growth of hair.
Extract in ginseng is extracted in a manner of enzymolysis and extraction, can retain the active principle in ginseng as far as possible,
After the compounding such as the same tuber of multiflower knotweed, ganoderma lucidum, promote the effect of natural on-off cycles of hair growth more obvious.
Active principle in enzyme-extracting solution is extracted again by ethyl alcohol, available sufficient correct hair growth result
More obvious extract.
Modified chitosan is compounded with Chinese herbal medicine extract, be can be improved and is waken up the time that the factor retains on scalp, into
And the lasting hair follicle stem cells on scalp are provided rich in the environment for waking up the factor.
Chitosan is modified using modified nano silver, can be further improved and wake up factor wake-up hair follicle stem cells
Quantity, and then improve hair increment.
The effective substance waken up in the factor can be further improved using modified nano-titanium dioxide modified nano silver
Matter rests on scalp, and then provides the approach effectively waken up for scalp for a long time.
The cordyceps sinensis take liquid the preparation method comprises the following steps: by after 8 mass parts supersonic wave wall breaking of cordyceps sinensis, be added
The ethyl alcohol of 500 mass parts 60% leaches 14 days, as cordyceps extracting liquid.
The lucidum extracting liquid the preparation method comprises the following steps: take 15 mass parts of ganoderma lucidum, the water of 10 times of amounts are added, it is conventional fried
30min filters to get filtrate, and the water of 10 times of amounts is added into filter residue, fried 30 minutes again, filtrate is obtained by filtration, merges twice
Filtrate, then into filtrate be added 0.1% chitin type flocculant, it is to be precipitated completely after, filtering, filtrate is lucidum extracting liquid.
The tuber of multiflower knotweed extracting solution the preparation method comprises the following steps: take 5 mass parts of the tuber of multiflower knotweed, add 10 times of amount water, fried 30min is filtered
Filtrate adds 10 times of amount water into filter residue, again fried 30min, and filtrate is obtained by filtration, and merges filtrate twice, then into filtrate
0.1% chitin type flocculant is added, after band precipitating is complete, filtering, filtrate is tuber of multiflower knotweed extracting solution.
A kind of hair follicle stem cells wake up the preparation method of the factor, and cordyceps extracting liquid, lucidum extracting liquid, the tuber of multiflower knotweed is taken to extract
Liquid, Ginseng extract, chitosan after being sufficiently mixed, stir evenly, 37 DEG C of water-bath 1.5h.
The extracting solution of Chinese herbal medicine is simply mixed, the effect after not only having remained combined Chinese herb with chitosan, but also can grown
Effect is that scalp provides the environment of hair stem cells Proliferation, Differentiation.
Embodiment 2
A kind of hair follicle stem cells wake-up factor, comprising: 5 mass parts of cordyceps extracting liquid, 9 mass parts of lucidum extracting liquid, the tuber of multiflower knotweed
11 mass parts of extracting solution, 7 mass parts of Ginseng extract, 0.1 mass parts of chitosan.
The Ginseng extract the preparation method comprises the following steps:
S41. cellulase, pectase are add to deionized water, with the ginseng mixing thinly sliced, react 1h at 45 DEG C,
Obtain enzyme-extracting solution；
S42. 95% ethyl alcohol, 55 DEG C of reflux 3h is added for the first time into enzyme-extracting solution, filtering obtains first extract and residue, residual
The ethyl alcohol of second of addition 95% of slag extracts, and 55 DEG C of reflux 2h merge with first extract, obtain alcohol extract, the first time second
Alcohol additional amount: second of amount of alcohol added=1:4；
S43. alcohol extract is concentrated under reduced pressure at 50 DEG C into medicinal extract shape, ethyl alcohol is recycled in concentration process, obtained medicinal extract vacuum is dry
It is dry both to obtain ginseng extract；By ginseng extract to be added in ethyl alcohol, Ginseng extract, the ginseng extract: second are obtained
Alcohol=2:100.
The cellulase, pectase, deionized water, ginseng mass ratio be 5 × 10-4:10-3:1:4。
The ethyl alcohol is 1:0.4 with the mass ratio of enzyme-extracting solution.
The chitosan be modification of chitosan, the modification of chitosan the preparation method comprises the following steps:
S71. 10 mass parts of chitosan, 0.5 mass parts of nano silver, 2 mass parts of human-like collagen, 10 mass parts of glycerol, card are taken
1 mass parts of wave nurse, 0.6 mass parts of Geniposide, add chitosan into deionized water, stir evenly, and nano silver and sweet is added
Oil, ultrasonic disperse obtain mixed liquor；
S72. Geniposide is added in mixed liquor, reacts 36h at 35 DEG C, add carbomer, adjust solution to pH=7；
S73. human-like collagen is added, after mixing evenly, obtained solid substance score it is poly- must to be both modified shell by freeze-drying
Sugar.
The nano silver is modified Nano silver, the method for modifying of the nano silver are as follows:
S81. 1 mass parts of nano silver are taken, are added in the polyethylene glycol of 4 mass parts, ultrasonic disperse obtains nano silver dispersion；
S82. 1.5 mass parts of nano-titanium dioxide are taken, are added in the deionized water of 10 mass parts, ultrasonic disperse obtains nanometer two
Titanium oxide dispersion liquid；
S83. after nano silver dispersion being mixed with nano titanium oxide dispersion, ultrasonic disperse 1h, vacuum drying is modified
Nano silver.
The nano-titanium dioxide is modified nano-titanium dioxide, the method for modifying of the modified nano-titanium dioxide are as follows:
Tetraethyl titanate and nickel acetylacetonate are added in benzene, precursor solution is obtained, the concentration of the tetraethyl titanate is 0.5mol/L,
The concentration of the nickel acetylacetonate is 0.01mol/L；Precursor solution is assisted with the charging rate of 3ml/min through external oxygen
Shearing atomization burner forms fine atomized drop, and microlayer model is in H2/O2Nanometer powder, institute are converted under the auxiliary of diffusion flame
Stating the shear pressure at shearing atomization mouthpiece is 0.2MPa, H in the diffusion flame2Flow be 150L/h, O2Flow be
700L/h；Nanometer powder is calcined into 3h in air atmosphere, calcination temperature is 300 DEG C, obtains modifying titanium dioxide after cooling.
A kind of hair follicle stem cells wake up the preparation method of the factor, and cordyceps extracting liquid, lucidum extracting liquid, the tuber of multiflower knotweed is taken to extract
Liquid, Ginseng extract, chitosan after being sufficiently mixed, stir evenly, 37 DEG C of water-bath 1h.
Embodiment 3
A kind of hair follicle stem cells wake-up factor, comprising: 10 mass parts of cordyceps extracting liquid, 12 mass parts of lucidum extracting liquid are first
Black 15 mass parts of extracting solution, 9 mass parts of Ginseng extract, 0.5 mass parts of chitosan.
The Ginseng extract the preparation method comprises the following steps:
S41. cellulase, pectase are add to deionized water, with the ginseng mixing thinly sliced, react 1h at 45 DEG C,
Obtain enzyme-extracting solution；
S42. 95% ethyl alcohol, 55 DEG C of reflux 3h is added for the first time into enzyme-extracting solution, filtering obtains first extract and residue, residual
The ethyl alcohol of second of addition 95% of slag extracts, and 55 DEG C of reflux 2h merge with first extract, obtain alcohol extract, the first time second
Alcohol additional amount: second of amount of alcohol added=1:4；
S43. alcohol extract is concentrated under reduced pressure at 50 DEG C into medicinal extract shape, ethyl alcohol is recycled in concentration process, obtained medicinal extract vacuum is dry
It is dry both to obtain ginseng extract；By ginseng extract to be added in ethyl alcohol, Ginseng extract, the ginseng extract: second are obtained
Alcohol=5:100.
The cellulase, pectase, deionized water, ginseng mass ratio be 5 × 10-4:10-3:1:4。
The ethyl alcohol is 1:0.4 with the mass ratio of enzyme-extracting solution.
The chitosan be modification of chitosan, the modification of chitosan the preparation method comprises the following steps:
S71. 100 mass parts of chitosan, 2 mass parts of nano silver, 10 mass parts of human-like collagen, 50 mass parts of glycerol, card are taken
2 mass parts of wave nurse, 1.5 mass parts of Geniposide, add chitosan into deionized water, stir evenly, and nano silver and sweet is added
Oil, ultrasonic disperse obtain mixed liquor；
S72. Geniposide is added in mixed liquor, reacts 48h at 40 DEG C, add carbomer, adjust solution to pH=7；
S73. human-like collagen is added, after mixing evenly, obtained solid substance score it is poly- must to be both modified shell by freeze-drying
Sugar.
The nano silver is modified Nano silver, the method for modifying of the nano silver are as follows:
S81. 2 mass parts of nano silver are taken, are added in the polyethylene glycol of 6 mass parts, ultrasonic disperse obtains nano silver dispersion；
S82. 3 mass parts of nano-titanium dioxide are taken, are added in the deionized water of 15 mass parts, ultrasonic disperse obtains nano-silica
Change titanium dispersion liquid；
S83. after nano silver dispersion being mixed with nano titanium oxide dispersion, ultrasonic disperse 5h, vacuum drying is modified
Nano silver.
The nano-titanium dioxide is modified nano-titanium dioxide, the method for modifying of the modified nano-titanium dioxide are as follows:
Tetraethyl titanate and nickel acetylacetonate are added in benzene, precursor solution is obtained, the concentration of the tetraethyl titanate is 0.8mol/L,
The concentration of the nickel acetylacetonate is 0.3mol/L；Precursor solution is assisted with the charging rate of 10ml/min through external oxygen
Shearing atomization burner forms fine atomized drop, and microlayer model is in H2/O2Nanometer powder, institute are converted under the auxiliary of diffusion flame
Stating the shear pressure at shearing atomization mouthpiece is 0.3MPa, H in the diffusion flame2Flow be 300L/h, O2Flow be
1200L/h；Nanometer powder is calcined into 6h in air atmosphere, calcination temperature is 500 DEG C, obtains modifying titanium dioxide after cooling.
A kind of hair follicle stem cells wake up the preparation method of the factor, and cordyceps extracting liquid, lucidum extracting liquid, the tuber of multiflower knotweed is taken to extract
Liquid, Ginseng extract, chitosan after being sufficiently mixed, stir evenly, 37 DEG C of water-bath 2h.
Embodiment 4
Embodiment 4 with embodiment 1 the difference is that, the Ginseng extract the preparation method comprises the following steps: take 30 mass parts of ginseng, cut
60% ethyl alcohol of 800 mass parts is added in piece, leaches 10 days, and it is Ginseng extract that leachate, which is obtained by filtration,.
Embodiment 5
Embodiment 5 with embodiment 1 the difference is that, the chitosan is unmodified.
Embodiment 6
Embodiment 6 with embodiment 1 the difference is that, the nano silver is unmodified.
Embodiment 7
Embodiment 7 with embodiment 1 the difference is that, the nano-titanium dioxide is unmodified.
Comparative example 1
Comparative example 1 with embodiment 1 the difference is that, the wake-up factor only includes 7 mass parts of cordyceps extracting liquid.
Comparative example 2
Comparative example 2 with embodiment 1 the difference is that, the wake-up factor only includes 10 mass parts of lucidum extracting liquid.
Comparative example 3
Comparative example 3 with embodiment 1 the difference is that, the wake-up factor only includes 12 mass parts of tuber of multiflower knotweed extracting solution.
Comparative example 4
Comparative example 4 with embodiment 1 the difference is that, the wake-up factor only includes 8 mass parts of Ginseng extract.
Comparative example 5
Comparative example 5 with embodiment 1 the difference is that, the wake-up factor only includes 0.2 mass parts of chitosan.
Experimental example
The wake-up factor in embodiment 1 ~ 7 and in comparative example 1 ~ 5 is applied in the treatment of hair loss patient, the production of each comparative example
Product are applied to 50 people, and the age 35~50 years old, wherein 35 people of male, 15 people of women, by liquid even application in hair loss area root of hair
Place, once a day.
Observe patient: scalp elasticity improves；Hair quality becomes strong (hair is hardened, glossy)；Hair follicle increase (is seen under microscope
It examines)；Kainogenesis is at (microscopically observation).
By the above therapeutic scheme, 3 months after treatment results are observed respectively, are recorded, as a result as shown in table 1:
The variation of 1 patient's hair quality of table
It as known from Table 1, can be with using three months after joined the extracting solution of cordyceps sinensis, the tuber of multiflower knotweed, ganoderma lucidum, ginseng with chitosan
The hair-growing effects to be worked well, while the preparation method of preferred Ginseng extract, and chitosan is modified,
Hair quality can further be improved, accelerate new generation.
In embodiment 1 ~ 3, using the Ginseng extract of modified chitosan and enzymolysis and extraction, the therapeutic effect of alopecia
Preferably, all patients generate new hair after 3 months, and original hair quality has all obtained certain improvement.
Ginseng extract in embodiment 4 and without enzymolysis and extraction, but ethyl alcohol extracts after slice, hair growth
Effect have certain difference with embodiment 1 ~ 3, show that the Ginseng extract of enzymolysis and extraction can be further improved controlling for alopecia
Therapeutic effect.
Chitosan in embodiment 5, unmodified, the effect of hair growth is weaker than embodiment 6 and 7, shows changing for chitosan
Property be improve hair growth effect more important factor, therefore, chitin modified is extremely necessary.
The wake-up factor in comparative example 1 ~ 4 is single substance, and the effect of hair growth is weaker than embodiment 1 ~ 7,
Show that single substance is can not to provide the effect of preferable hair growth, it is necessary to which many kinds of substance compounding could generate good control
Treat the effect of alopecia.
Claims (10)
1. a kind of hair follicle stem cells wake up the factor characterized by comprising 5 ~ 10 mass parts of cordyceps extracting liquid, ganoderma lucidum mention
Take 9 ~ 12 mass parts of liquid, 11 ~ 15 mass parts of tuber of multiflower knotweed extracting solution, 7 ~ 9 mass parts of Ginseng extract, 0.1 ~ 0.5 mass parts of chitosan.
2. a kind of hair follicle stem cells according to claim 1 wake up the factor characterized by comprising cordyceps sinensis extracts
7 ~ 10 mass parts of liquid, 10 ~ 12 mass parts of lucidum extracting liquid, 12 ~ 15 mass parts of tuber of multiflower knotweed extracting solution, 8 ~ 9 mass parts of Ginseng extract,
0.2 ~ 0.5 mass parts of chitosan.
3. a kind of hair follicle stem cells according to claim 2 wake up the factor characterized by comprising cordyceps sinensis extracts
7 mass parts of liquid, 10 mass parts of lucidum extracting liquid, 12 mass parts of tuber of multiflower knotweed extracting solution, 8 mass parts of Ginseng extract, 0.2 matter of chitosan
Measure part.
4. a kind of hair follicle stem cells according to claim 1 wake up the factor, which is characterized in that the system of the Ginseng extract
Preparation Method are as follows:
S41. cellulase, pectase are add to deionized water, with the ginseng mixing thinly sliced, react 1h at 45 DEG C,
Obtain enzyme-extracting solution；
S42. 95% ethyl alcohol, 55 DEG C of reflux 3h is added for the first time into enzyme-extracting solution, filtering obtains first extract and residue, residual
The ethyl alcohol of second of addition 95% of slag extracts, and 55 DEG C of reflux 2h merge with first extract, obtain alcohol extract, the first time second
Alcohol additional amount: second of amount of alcohol added=1:4；
S43. alcohol extract is concentrated under reduced pressure at 50 DEG C into medicinal extract shape, ethyl alcohol is recycled in concentration process, obtained medicinal extract vacuum is dry
It is dry both to obtain ginseng extract；By ginseng extract to be added in ethyl alcohol, Ginseng extract, the ginseng extract: second are obtained
Alcohol=2 ~ 5:100.
5. a kind of hair follicle stem cells according to claim 4 wake up the factor, which is characterized in that the cellulase, pectin
Enzyme, deionized water, ginseng mass ratio be 5 × 10-4:10-3:1:4。
6. a kind of hair follicle stem cells according to claim 4 wake up the factor, which is characterized in that the same enzymolysis and extraction of ethyl alcohol
The mass ratio of liquid is 1:0.4.
7. a kind of hair follicle stem cells according to claim 1 wake up the factor, which is characterized in that the chitosan is modified shell
Glycan, the modification of chitosan the preparation method comprises the following steps:
S71. 10 ~ 100 mass parts of chitosan are taken, 0.5 ~ 2 mass parts of nano silver, 2 ~ 10 mass parts of human-like collagen, glycerol 10 ~
50 mass parts, 1 ~ 2 mass parts of carbomer, 0.6 ~ 1.5 mass parts of Geniposide add chitosan into deionized water, and stirring is equal
It is even, nano silver is added and glycerol, ultrasonic disperse obtain mixed liquor；
S72. Geniposide is added in mixed liquor, 36 ~ 48h is reacted at 35 ~ 40 DEG C, adds carbomer, adjust solution to
pH=7；
S73. human-like collagen is added, after mixing evenly, obtained solid substance score it is poly- must to be both modified shell by freeze-drying
Sugar.
8. a kind of hair follicle stem cells according to claim 7 wake up the factor, which is characterized in that the nano silver is that modification is received
Meter Yin, the method for modifying of the nano silver are as follows:
S81. 1 ~ 2 mass parts of nano silver are taken, are added in the polyethylene glycol of 4 ~ 6 mass parts, ultrasonic disperse, nano silver dispersion is obtained
Liquid；
S82. 1.5 ~ 3 mass parts of nano-titanium dioxide are taken, are added in the deionized water of 10 ~ 15 mass parts, ultrasonic disperse is received
Rice titanium oxide dispersion；
S83. after nano silver dispersion being mixed with nano titanium oxide dispersion, 1 ~ 5h of ultrasonic disperse, vacuum drying is changed
Property nano silver.
9. a kind of hair follicle stem cells according to claim 8 wake up the factor, which is characterized in that the nano-titanium dioxide is
Modified nano-titanium dioxide, the method for modifying of the modified nano-titanium dioxide are as follows: tetraethyl titanate and nickel acetylacetonate are added
Into benzene, precursor solution is obtained, the concentration of the tetraethyl titanate is 0.5 ~ 0.8mol/L, and the concentration of the nickel acetylacetonate is
0.01~0.3mol/L；Precursor solution is assisted into shearing atomization burner shape through external oxygen with the charging rate of 3 ~ 10ml/min
At fine atomized drop, microlayer model is in H2/O2Nanometer powder is converted under the auxiliary of diffusion flame, the shearing is atomized mouthpiece
The shear pressure at place is 0.2 ~ 0.3MPa, H in the diffusion flame2Flow be 150 ~ 300L/h, O2Flow be 700 ~
1200L/h；Nanometer powder is calcined into 3 ~ 6h in air atmosphere, calcination temperature is 300 ~ 500 DEG C, and modified dioxy is obtained after cooling
Change titanium.
10. the preparation method that a kind of hair follicle stem cells wake up the factor, which is characterized in that cordyceps extracting liquid, ganoderma lucidum is taken to extract
Liquid, tuber of multiflower knotweed extracting solution, Ginseng extract, chitosan, after being sufficiently mixed, stir evenly, 37 DEG C of 1 ~ 2h of water-bath.
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| CN111956521A (en) * | 2020-09-16 | 2020-11-20 | 上海海盈通感光材料有限公司 | Hair care composition and application thereof, hair conditioner and scalp essence adopting composition and preparation method thereof |
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Address after: Building F2, 39 Ruihe Road, Huangpu District, Guangzhou City, Guangdong Province 510000 Applicant after: Lancy Purcell Biotechnology (Guangzhou) Co.,Ltd. Address before: 510000 Room D09, 4th Floor, 131 Airport Road, Baiyun District, Guangzhou City, Guangdong Province Applicant before: Lancy Purcell Biotechnology (Guangzhou) Co.,Ltd. |
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Application publication date: 20190719 |