CN1895294A - Chinese pilose spore concentrated oral liquid - Google Patents
Chinese pilose spore concentrated oral liquid Download PDFInfo
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- CN1895294A CN1895294A CNA2006100406684A CN200610040668A CN1895294A CN 1895294 A CN1895294 A CN 1895294A CN A2006100406684 A CNA2006100406684 A CN A2006100406684A CN 200610040668 A CN200610040668 A CN 200610040668A CN 1895294 A CN1895294 A CN 1895294A
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- china pilose
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- 238000000926 separation method Methods 0.000 claims abstract description 4
- 238000000855 fermentation Methods 0.000 claims description 39
- 230000004151 fermentation Effects 0.000 claims description 39
- 241000190633 Cordyceps Species 0.000 claims description 19
- 238000004519 manufacturing process Methods 0.000 claims description 19
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
An oral liquid for sedation, promoting sleep, dilating trachea, lowering blood pressure and improving immunity is prepared from Chinese hirsutella sinensis through fermenting, centrifugal separation, filtering, filling it in containers, sterilizing and packing.
Description
Technical field
The present invention relates to a kind of oral liquid, specifically the concentrated oral liquid of producing by the phorozoon-China pilose spore of Cordyceps.
Background technology
Modern society, along with the continuous increase of the people life style and the social pressure of bearing, sleep quality becomes weighs the whether healthy important symbol of people.People's study, life have been had a strong impact on owing to sleep quality descends that the insomnia cause, forgetful, dreaming often and waking easily, cardiopalmus are nervous, dizziness and tinnitus etc.Show that according to Epidemiological study the sickness rate of having a sleepless night in the American population to 2002 is 32%-35%, Britain is 10%-14%, and Japan is 20%, does not still have definite data in China, but according to the part information statistics, insomniac's rate of seeing a doctor reaches more than 10%, and increases year by year.Along with the fierceness day by day of social competition, people's rhythm of life is constantly accelerated, and the morbidity crowd who causes also further expands to middle age by the old people because sleep quality descends, or even population of adolescent.Therefore, solution sleep quality problem is imperative.
Modern medicine field, the means of Cure for insomnia are transferred to present psychotherapy, Diet Therapy, Drug therapy and are combined, and obtain very big effect by in the past the Drug therapy of sleeping peacefully of depending merely on.And, be easy to generate dependency, drug resistance, toxicity owing to take sleeping pill for a long time, so that gastrointestinal dysfunction, even cause danger; Simultaneously, sleeping pill can not fundamentally improve, improve sleep quality.So now increasing people tends to the natural drug Cure for insomnia that uses nonhazardous, have no side effect, thereby reach the purpose of " treating both the principal and secondary aspects of a disease ".
Calm the nerves in the market abirritative medicine and health product mainly contain following:
Melatonin (key special Bioceuticals Inc. in Wuxi produces), ANSHEN BUNAO YE (Aodong, Jilin is prolonged the limit Pharmaceutical and produced), NAOXINSHU oral liquid (Shijiazhuang Shineway Pharmaceutical Co., Ltd's production), vigor oral liquid for relaxation (production of Sichuan buchu group), sweet dreams oral liquid (Yantai Rongchang Pharmaceutical Co., Ltd.'s production), Mrs's oral liquid (Shenzhen Taitai Pharmaceutical Industry Co. Ltd.'s production), QINGCHUN BAO antiageing tablet (Zhengda Group's production).
In recent years, a collection of Chinese caterpillar fungus health food constantly goes on the market.Main oral liquid product has following several:
Rich cordyceps militaris oral liquid difficult to understand, has kidney tonifying, tonifying the lung, nourish blood, make eye bright, tonify deficiency effect (Jiangsu Shenhua Medicine Co., Ltd's production), natural sciences Cordyceps king oral liquor, to respiratory tract disease, nephropathy, the insomnia that cardiovascular disease and neurasthenia cause, anorexia etc. have certain preventive and therapeutic effect (production of Zhongshan city's natural sciences cordyceps product company limited), the compound Chinese caterpillar fungus oral liquid, can promote and improve immunity, prolong cell survival, has resisting fatigue, effect such as cold-resistant grade, improve the symptom of suffering from a deficiency of the kidney, flat cloperastine is breathed heavily to wait all has obvious effect (the national new drug of Renhe, Jiangxi Pharmaceutical), the Chinese caterpillar fungus extract oral liquid, Chinese caterpillar fungus extract royal jelly oral liquid (production of Zhejiang Prov. Chinese Medicine Inst pharmaceutical factory), mend king Cordyceps crystalline substance (Lanzhou science and technology biological factory production).
Though the said goods is using names Cordyceps all, all not by real Cordyceps phorozoon---China pilose spore fermenting and producing, and be complex agent type substantially.
Summary of the invention
The objective of the invention is: a kind of phorozoon of utilizing Cordyceps is provided---China pilose spore is developed out a kind of multiple functions such as calmness, hypnosis, expansion trachea, blood pressure lowering that have, it can also regulate immunologic function simultaneously, strengthens the China pilose spore concentrated oral liquid for health care of the resistance against diseases of human body.
Concrete technical scheme is as follows:
The China pilose spore concentrated oral liquid phorozoon-China pilose spore strain of the Cordyceps of separation and purification, by one level solid flask strain culture, secondary liquid shaking bottle seed culture, tertiary liquid fermentation, wherein tertiary liquid fermentation comprises three steps, first class seed pot is produced, the secondary seed jar is produced and fermentation tank production, make the China pilose spore fermentation liquid, the effective ingredient in the fermentation liquid comprises
Mannitol (mg/mL) 0.0670～0.0754
Polysaccharide (mg/mL) 0.1442～0.1470
Essential amino acids total content (mg/mL) 0.4358～0.4903
Adenosine (mg/mL) 0.0617～0.0635
Uridnine (mg/mL) 0.0726～0.0761
Guanosine (mg/mL) 0.1014～0.1025
At this moment, occur a large amount of little fungus ball in the fermentation liquid and find a large amount of mycelia through sediments microscope inspection, residual sugar is below 1%, and fermentation liquid is than thickness, and color is obviously deepened; Get the oral liquid raw material.
After above-mentioned fermentation liquid goes out jar, through centrifugal karusen via hole diameter after removing a large amount of mycelium is the ceramic membrane filter of 0.2 μ m, through the reverse osmosis membrane filtration and the degerming of molecular cut off about 300, the simmer down to volume is the fermentation liquid of original volume about 1/4th, promptly obtains concentrated oral liquid stock solution again;
This oral liquid is an extremely brown xanchromatic thick liquid of sundown, the color and luster uniformity, and putting for a long time to have trace muddy, the feeble QI raw meat, it is little sweet to distinguish the flavor of;
Its effective ingredient is:
Mannitol (mg/mL) 0.2680～0.3016
Polysaccharide (mg/mL) 0.5768～0.5880
Essential amino acids total content (mg/mL) 1.7432～1.9612
Adenosine (mg/mL) 0.2468～0.2540
Uridnine (mg/mL) 0.2904～0.3044
Guanosine (mg/mL) 0.4056～0.4100.
With concentrated oral liquid stock solution be filled into bottle, sterilization, lamp inspection, pack product; Sterilising conditions: temperature is 121 ℃, time 25-35 minute; About terms of packing: temperature 20-25 ℃, relative humidity 40～45%.
Cordyceps is a kind of as medicinal fungus, in Traditional Chinese Medicine hundred flavors, be considered to a kind of treasured of rare Chinese medicine with the magical effect of " controlling weakness ", " equilibrium between yin and yang ", " protect lung kidney tonifying, replenishing vital essence and invigorating vital QI, specially mend the gate of vitality " just and the China that makes a name enjoys great prestige abroad.
In recent years, domestic and international artificial culture Cordyceps and it is developed to health food etc. has been a big focus, but no matter be research or cultivation, all the phorozoon that mainly depends on Cordyceps is as strain, so the conclusive evidence problem of Cordyceps phorozoon just has extremely important meaning.Along with the modern molecular biology development and in conjunction with other multiple affirmation means, the researcher reaches a conclusion: the phorozoon of Cordyceps is China pilose spore (Hirsutella sinensis), and this point is generally acknowledged by the whole world at present.A large amount of studies show that contains in its fermentation liquid outside the human body necessary several amino acids and trace element to China pilose spore for we, also contain cordycepic acid, cordycepin, Cordyceps polysaccharide and several physiological active substances, have multiple functions such as calmness, hypnosis, expansion trachea, blood pressure lowering, it can also regulate immunologic function simultaneously, strengthens the resistance against diseases of human body.
The concentrated type concentrated oral liquid is with Cordyceps phorozoon---and the China pilose spore fermentation liquid is a raw material, and the report that is applied to aspects such as drug manufacture, exploitation at present both at home and abroad about the China pilose spore fermentation liquid does not almost have, and has caused a large amount of losses of fermentation liquid in the Chinese caterpillar fungus fermentation.We are with the fermentation liquid of fermentation mode mass production Cordyceps phorozoon bacterial strain China pilose spore, and with its be developed to calmness, the hypnosis concentrated oral liquid will be filled up present health-product market blank.And the suitability for industrialized production China pilose spore can improve enterprise and value-added content of product, reduces cost, and is the road of living resources sustainable use, will obtain bigger economic benefit and social benefit.
With the China pilose spore fermentation liquid is that the concentrated oral liquid of raw material is a kind of fermented product of pure natural, does not add any additives and adjuvant, is different from other medicine and is the health product of raw material with the Chinese crude drug.Through a large amount of experimental studies have found that,, also have no side effect even the human body high dose sucks, show that through functional experiment this concentrated oral liquid has significant calmness, syngignoscism, can fundamentally improve the sleep quality of human body, can take suitable all kinds of crowds, and no dependence for a long time.
Carry out the production of China pilose spore concentrated oral liquid by biotechnology, its mycelium is the important source material (making capsule as hundred) that bio-pharmaceuticals and health product are produced, and fermentation liquid can be produced and is concentrated oral liquid, it is a kind of environmental type industry, both there be not the waste water waste material, and can fully utilize, achieve many things at one stroke.
The specific embodiment
Below in conjunction with embodiment, the present invention is done to describe further:
Embodiment:
Strain: Cordyceps (Cordyceps sinensis (Berk) Sacc.) the isolated China pilose spore RCEF0273 of the Stroma bacterial strain (Hirsutella sinensis Liu, Guo, the Yu ﹠amp that gather from Qinghai; Zeng), be the phorozoon of Cordyceps and with the molecular biology method conclusive evidence.Strain of the present invention is preserved as the microorganism that is used for proprietary program by China Committee for Culture Collection of Microorganisms common micro-organisms center, preservation date: on June 5th, 2003, preserving number: CGMCC 0940, classification name: China pilose spore.
The production process of China pilose spore concentrated oral liquid is as follows:
(1) liquid fermentation process
1., one level solid flask strain culture
(culture medium is: Rhizoma Solani tuber osi 200g liquor, glucose 20g, maltose 10g, peptone 10g, yeast powder 10g, MgSO in 50ml triangular flask culture medium with the bacterial classification inoculation of separation and purification
41.5g, KH
2PO
43g, agar 20g, add water to 1L, pH6.5), put into 19 ℃ of incubators, incubation time is 20 days, treats that mycelia is covered with triangular flask and produces spore to get final product;
2., secondary liquid shaking bottle seed culture
The 200ml culture medium of packing in the 500ml triangular flask (contains glucose 20g, dried silkworm chrysalis meal 30g, yeast powder 20g, MgSO in the 1000ml culture medium
40.5g, KH
2PO
41g adds water after each component is mixed and mends to 1000ml, and is 6.5 with saturated NaOH adjust pH), use 1.2Kg/cm
2Autoclaving 30 minutes, to be cooled during to room temperature, the bacterial classification inoculation of 2 bottles of solid triangular flasks breaing up on 500ml triangular flask culture medium, and is placed the constant-temperature shaking culture case, 19 ± 1 ℃ of temperature are cultivated under 180 rev/mins of conditions, and incubation time is 10 days～12 days;
3., tertiary liquid fermentation:
A, first class seed pot production
The 21L fluid medium of packing in the 50L airlift fermentor (contains white sugar 20g, dried silkworm chrysalis meal 30g, yeast powder 30g, MgSO in the 1000ml culture medium
40.5g, KH
2PO
41g adds water after each component is mixed and mends to 1000ml, and transfers pH to 6.5 with saturated NaOH), the culture medium temperature is 6.0～7.0 greater than 95 ℃, pH value, and adding edible defoaming agent-polyoxypropylene ethylene oxide glycerin ether, addition is 0.03%, 14.7 * 10
4Under the Pa pressure, feed Steam Heating to 121 ℃, and pressurize 30～45 minutes; After the sterilization, when being cooled to 20 ℃ 4 bottles of above-mentioned cultured 500ml shake-flask seeds are inserted cultivation, cultivation temperature is 19-20 ℃, and ventilation is 1: 0.5V/Vmin, pressurize 4.903～7.0845 * 10
4Pa through 7 days aerobic culture, reaches the logarithmic growth after date and gets final product, and final volume of culture is 30L;
B, secondary seed jar are produced
The 300L fluid medium (the same) of in the 500L seed tank, packing into, pH value is 6.0～7.0, and adds edible defoaming agent (the same), and addition is 0.03%, and original position is heated to 121 ℃, 14.7 * 10
4Under the Pa pressure, pressurize 30～45 minutes; After the sterilization, when being cooled to 20 ℃, the 30L liquid seeds of first class seed pot all being inserted the secondary seed jar cultivate, cultivation temperature 19-20 ℃, ventilation is 1: 0.5V/Vmin, pressurize 4.903～7.0845 * 10
4Pa through 7 days aerobic culture, reaches the logarithmic growth after date and gets final product, and final volume of culture is 300L;
C, fermentation tank production
The 3T fluid medium (the same) of in 5T fermentation tank 3, packing into, pH value is 6.0～7.0, and add edible defoaming agent (the same), and addition is 0.03%, original position is heated to 121 ℃, pressurize 40 minutes, be cooled to 20 ℃, cultured liquid seeds 300L all inserts fermentor cultivation with the secondary seed jar, and cultivation temperature is 19-20 ℃, ventilation is 1: 0.4～0.5V/Vmin, tank pressure 4.903～7.0845 * 10
4Pa, incubation time 5 days;
A large amount of little fungus ball occur and find a large amount of mycelia through sediments microscope inspection in fermentation liquid, residual sugar is below 1%, and fermentation liquid is than thickness, and color is obviously deepened, and can go out jar.
Effective ingredient in the fermentation liquid comprises:
Mannitol (mg/mL) 0.0670～0.0754
Polysaccharide (mg/mL) 0.1442～0.1470
Essential amino acids total content (mg/mL) 0.4358～0.4903
Adenosine (mg/mL) 0.0617～0.0635
Uridnine (mg/mL) 0.0726～0.0761
Guanosine (mg/mL) 0.1014～0.1025
After karusen goes out jar, with peristaltic pump karusen is pumped into the high speed tube centrifuge, karusen is removed a large amount of mycelium behind 15000 rev/mins of condition high speed centrifugations, collect the oral liquid raw material.
(2) production of concentrated oral liquid
1. filtration sterilization remove impurity
After described fermentation liquid goes out jar, with peristaltic pump fermentation liquid is pumped into the high speed tube centrifuge, fermentation liquid is removed a large amount of mycelium behind 15000 rev/mins of high speed centrifugations, via hole diameter is the ceramic membrane filter of 0.2 μ m, further removes mycelium residual in the fermentation liquid and particle; And then through the reverse osmosis membrane filtration and the degerming of molecular cut off about 300, the simmer down to volume is the fermentation liquid of original volume 1/4th.Through above-mentioned processing, promptly obtain concentrated oral liquid stock solution.
2. the cleaning of container, sterilization
The 10ml vial is put into full-automatic bottle washer, when bottle is delivered to the porch by conveyer belt, at first enter soaking compartment, 30～40 ℃ thermokalite water is arranged in the soaking compartment, through immersion after a while, bottle tentatively obtains cleaning and sterilization under the effect of aqueous alkali.Then bottle is the immersion and the large-area jet cleaning of shower nozzle of 70～75 ℃ of water through temperature in the soaking compartment, enters washing liquid hydro-peening part then.In this section, bottle is subjected to the powerful hydro-peening of high pressure washing liquid, can remove all dirts.After this, enter the cleaning temperature-fall period, in this process, bottle is through the superheated water hydro-peening, and warm water hydro-peening and cold water hydro-peening three phases are finished whole wash bottle process.
Automatically enter 160 ℃ dry sterilization tunnel through above " three water, three gas " washed glass bottle, can use behind about 30min that sterilizes.
3. fill:
With the raw material for preparing in the material-compound tank, squeeze into gravity tank with pump, adjust the amount of fill head, carry out filling and capping.Fill 10ml in every vial.
4. terminal sterilization:
After the oral liquid bottle fill is good, be 121 ℃ through sterilization cabinet temperature, sterilization about 30 minutes time.
5. lamp inspection:
To be arranged in a row check mouth through the bottle of filling and sealing through oral liquid lamp inspection machine, bottle back adularescent light transillumination, the bottle front has magnifier to amplify.To in product-filled liquid level height, the liquid whether impurity, clarity being arranged, seal quality etc. are checked, thereby are guaranteed product-filled quality by the lamp inspection machine.
6. pack
The product that is up to the standards is packed, and general about 20 ℃ of the temperature that keep during packing are in the environment of relative humidity 40～45%.
The China pilose spore concentrated oral liquid is an extremely brown xanchromatic thick liquid of sundown, the color and luster uniformity, and putting for a long time to have trace muddy.The feeble QI raw meat, it is little sweet to distinguish the flavor of.
Its main effective ingredient is:
Mannitol (mg/mL) 0.2680～0.3016
Polysaccharide (mg/mL) 0.5768～0.5880
Essential amino acids total content (mg/mL) 1.7432～1.9612
Adenosine (mg/mL) 0.2468～0.2540
Uridnine (mg/mL) 0.2904～0.3044
Guanosine (mg/mL) 0.4056～0.4100.
Claims (3)
1, China pilose spore concentrated oral liquid, phorozoon-China pilose spore strain with the Cordyceps of separation and purification, by one level solid flask strain culture, secondary liquid shaking bottle seed culture, tertiary liquid fermentation, wherein tertiary liquid fermentation comprises three steps, first class seed pot is produced, the secondary seed jar is produced and fermentation tank production, make the China pilose spore fermentation liquid, it is characterized in that:
Effective ingredient in the described fermentation liquid comprises
Mannitol (mg/mL) 0.0670～0.0754
Polysaccharide (mg/mL) 0.1442～0.1470
Essential amino acids total content (mg/mL) 0.4358～0.4903
Adenosine (mg/mL) 0.0617～0.0635
Uridnine (mg/mL) 0.0726～0.0761
Guanosine (mg/mL) 0.1014～0.1025
At this moment, occur a large amount of little fungus ball in the fermentation liquid and find a large amount of mycelia through sediments microscope inspection, residual sugar is below 1%, and fermentation liquid is than thickness, and color is obviously deepened; Get the oral liquid raw material.
2, China pilose spore concentrated oral liquid according to claim 1 is characterized in that:
After described fermentation liquid goes out jar, through centrifugal karusen via hole diameter after removing a large amount of mycelium is the ceramic membrane filter of 0.2 μ m, through the reverse osmosis membrane filtration and the degerming of molecular cut off about 300, the simmer down to volume is the fermentation liquid of original volume about 1/4th, promptly obtains concentrated oral liquid stock solution again;
This oral liquid is an extremely brown xanchromatic thick liquid of sundown, the color and luster uniformity, and putting for a long time to have trace muddy, the feeble QI raw meat, it is little sweet to distinguish the flavor of;
Its effective ingredient is:
Mannitol (mg/mL) 0.2680～0.3016
Polysaccharide (mg/mL) 0.5768～0.5880
Essential amino acids total content (mg/mL) 1.7432～1.9612
Adenosine (mg/mL) 0.2468～0.2540
Uridnine (mg/mL) 0.2904～0.3044
Guanosine (mg/mL) 0.4056～0.4100.
3, China pilose spore concentrated oral liquid according to claim 1 is characterized in that: with concentrated oral liquid stock solution be filled into bottle, sterilization, lamp inspection, pack product; Sterilising conditions: temperature is 121 ℃, time 25-35 minute; About terms of packing: temperature 20-25 ℃, relative humidity 40～45%.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102835728A (en) * | 2010-08-13 | 2012-12-26 | 内蒙古昆明卷烟有限责任公司 | Applications of active ingredients of cordyceps sinensis in cigarettes |
| CN103392513A (en) * | 2013-08-14 | 2013-11-20 | 西藏现代农业有限公司 | Ferment of aweto mycelia and application of ferment |
| CN104041809A (en) * | 2014-06-11 | 2014-09-17 | 沈南英 | Cordyceps sinensis fungal fermentation liquid ferment and preparation method thereof |
| CN105148029A (en) * | 2015-07-08 | 2015-12-16 | 萧县创亿畜禽养殖农民专业合作社 | Method for preparing cordyceps sinensis oral liquid |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1036531C (en) * | 1994-08-25 | 1997-11-26 | 俞永信 | Deep fermentation technology for cordyceps sinensis sacc |
| CN1055967C (en) * | 1996-12-13 | 2000-08-30 | 张世伟 | Cordyceps sinensis health-care nutrient liquor produced by submerged fermentation method |
| CN1478886A (en) * | 2003-06-14 | 2004-03-03 | 安徽林苑虫草研究所 | Chinese beimao spore liquid culture fermentationi technology |
-
2006
- 2006-05-22 CN CNB2006100406684A patent/CN100448456C/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102835728A (en) * | 2010-08-13 | 2012-12-26 | 内蒙古昆明卷烟有限责任公司 | Applications of active ingredients of cordyceps sinensis in cigarettes |
| CN103392513A (en) * | 2013-08-14 | 2013-11-20 | 西藏现代农业有限公司 | Ferment of aweto mycelia and application of ferment |
| CN104041809A (en) * | 2014-06-11 | 2014-09-17 | 沈南英 | Cordyceps sinensis fungal fermentation liquid ferment and preparation method thereof |
| CN105148029A (en) * | 2015-07-08 | 2015-12-16 | 萧县创亿畜禽养殖农民专业合作社 | Method for preparing cordyceps sinensis oral liquid |
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