CN111345419A - Compound fermentation type shark cartilage powder health drink - Google Patents
Compound fermentation type shark cartilage powder health drink Download PDFInfo
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- CN111345419A CN111345419A CN202010313462.4A CN202010313462A CN111345419A CN 111345419 A CN111345419 A CN 111345419A CN 202010313462 A CN202010313462 A CN 202010313462A CN 111345419 A CN111345419 A CN 111345419A
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
- A23L2/382—Other non-alcoholic beverages fermented
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
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- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a compound fermentation type shark cartilage powder health drink, belonging to the technical field of biology. According to the application, shark cartilage powder, kudzu roots and dendrobium are used as raw materials, and a modern bioseparation technology, a biological enzymolysis technology, a mixed bacteria microbial fermentation technology and a Chinese herbal medicine compatibility application technology are integrated to develop the shark cartilage powder health drink with high immunity enhancement capability, wherein the raw materials comprise 100 parts of shark cartilage powder, 2-5 parts of kudzu roots and 1-2 parts of dendrobium in a compatibility ratio; the mixed aerobic fermentation strain mainly comprises Aspergillus niger, beer yeast and Bacillus licheniformis; the shark cartilage powder health drink is rich in various functional active substances such as aminosugar, glycosaminoglycan, flavone, saponin and the like, has a remarkable immunity enhancement effect and a remarkable anti-tumor effect, is suitable for middle-aged and elderly consumer groups, is between health care wine and health care drink, and is a novel efficient health drink.
Description
Technical Field
The invention relates to a compound fermentation type shark cartilage powder health drink, in particular to a shark cartilage powder health drink with high immune health efficacy prepared by utilizing microbial synergistic fermentation, belonging to the technical field of biology.
Background
Shark is one of the oldest and longest-lived deep sea marine organisms in the world, has survived for four hundred million years on earth, has a perfect immune system, is extremely strong in vitality, is hardly infected by diseases, can recover wounds quickly even after being subjected to trauma, and has a cancer probability of about 1/million. The shark medicine in China is originally seen in the book Ben Cao Jing Ji Zhu, the cartilage of the shark medicine mainly comprises cartilage cells, collagen fibers, a large amount of proteoglycan and extracellular matrix, and the polysaccharide extracted from the shark cartilage is mainly used for clinically treating neuralgia, migraine, pain after abdominal cavity operation and the like. In developed countries such as Europe, America, Japan, etc., the shark cartilage polysaccharide is used as health food or health medicine for preventing and treating coronary heart disease, angina pectoris, myocardial infarction, etc. for a long time, and has no obvious toxic and side effects. In recent years, researches show that the shark cartilage contains various mucopolysaccharides and chondroitin sulfate besides minerals such as calcium, phosphorus, magnesium and the like, has remarkable anticoagulation, blood fat reduction, antiviral, anti-tumor and other functions, and is an unavailable natural food resource. Because the shark cartilage powder has the natural effects of promoting the regeneration of human cartilage and inhibiting new vessels, the shark cartilage powder is an excellent calcium-supplementing raw material. In recent years, the development of shark fin bone meal health food is rapidly developed, but the defects of single raw material, insufficient utilization of functional active ingredients, inconvenience in eating of the product and the like of the health product still exist, and the sustainable development of the shark fin bone meal health food industry is restricted.
The invention takes shark cartilage powder as a composition basis, combines Chinese herbal medicines such as kudzu root, dendrobium and the like, prepares a brand-new health drink with double health functions of the shark cartilage powder and the Chinese herbal medicines by utilizing compound microbial inoculum and performing compound fermentation on the Chinese herbal medicines with health treatment functions, expands the application approach of the shark cartilage powder, not only keeps the weight and recurs the weight and exerts the nutrition and health functions, but also overcomes the technical defect of low nutrition digestibility caused by more direct eating of the prior shark cartilage powder.
Disclosure of Invention
In order to overcome the defects of the prior art and realize the high-efficiency extraction of functional active substances and the remarkable improvement of the antioxidant capacity of the shark cartilage powder, the application provides a compound fermentation type shark cartilage powder health-care drink, integrates the modern bioseparation technology, the biological enzymolysis technology, the mixed bacteria microbial fermentation technology and the Chinese herbal medicine compatibility application technology, develops and develops the shark cartilage powder health-care drink with high immunity enhancement capability, meets the application requirements of the fields of health care, medical treatment, food and the like on the shark cartilage powder, has simple and high-efficiency fermentation process, strong immunity enhancement capability of the obtained product, remarkable anti-tumor effect, low production cost and easy industrial scale production.
The invention realizes the technical effects through the following technical scheme:
a compound fermentation type shark cartilage powder health drink is characterized in that kudzu root and dendrobium are added in the preparation process of the shark cartilage powder health drink, and the compatibility proportion is as follows: 100 parts of shark cartilage powder, 2-5 parts of kudzu root and 1-2 parts of dendrobium.
The Chinese herbal medicine raw materials are subjected to the following pre-extraction treatment: removing impurities from the raw materials of the kudzuvine root and the dendrobium, crushing, sieving by a sieve of 80-150 meshes, adding purified water with the mass ratio of 10 times of that of the raw materials of the Chinese herbal medicines, and soaking for 30min at the temperature of 40-60 ℃; adding cellulase accounting for 2% of the mass of the Chinese herbal medicine raw materials, extracting for 2min under the assistance of microwave with the microwave power of 250-400W, and standing for more than 30 min.
The application provides a compound fermentation type shark cartilage powder health drink which is characterized by being prepared by an aerobic fermentation process and a two-step anaerobic fermentation process, and the specific processes comprise:
(1) preparing fermentation liquor: mixing the pulverized shark cartilage powder with the pretreated Chinese herbal medicine raw materials, adding 20 times of purified water, and then adding 0.15-0.20 mass percent (NH) of fermentation liquor4)2SO4、0.10～0.15% KH2PO4、0.1%MgSO4；
(2) And (3) sterilization: sterilizing at 121 deg.C for 15 min;
(3) aerobic fermentation: adding a compound microbial agent with the mass ratio of 2-3% of the enzymolysis liquid into the enzymolysis liquid after enzymolysis, and performing aerobic fermentation for 6-12 h at the temperature of 28-35 ℃;
(4) anaerobic fermentation: after the aerobic fermentation is finished, adding 1-2% of lactobacillus powder, and carrying out anaerobic fermentation for 8-16 h at the temperature of 28-35 ℃;
(5) and (3) filtering: performing coarse filtration to remove fermentation thalli;
(6) centrifuging: centrifuging at 10000r/min, and separating supernatant;
(7) aging: ageing for 10-20 days at the temperature of 0-10 ℃ in order to promote the stability and clarification of the beverage;
(8) compounding: adding 1-1.5 g/L xylitol, 1-2 g/L isomaltose hypgather, 5-10 g/L citric acid, 5-10 g/L calcium lactate and 3-5 g/L sucrose;
(9) membrane filtration: filtering the shark cartilage powder health beverage with microporous membrane with pore size of 0.15 μm;
(10) and (3) sterile filling: filling and sealing in an aseptic filling production line to obtain the compound fermented shark cartilage powder health-care beverage.
The composite microbial agent in the step (3) is a mixed bacterium of aspergillus niger, saccharomyces cerevisiae and bacillus licheniformis, and the inoculation amount ratio is 1:2: 1.
The preparation method of the aspergillus niger seed liquid comprises the following steps: culturing for 24h at 35-40 deg.C in potato culture medium under aseptic condition to obtain Aspergillus niger seed liquid with effective viable count not less than 109cfu/mL。
The preparation method of the yeast seed liquid comprises the following steps: under the aseptic operation condition, two rings of strains are picked from the inclined plane of the yeast YDP by using an inoculating ring, inoculated into a 500mL triangular flask containing 300mLPDA liquid culture medium, subjected to shaking culture at the temperature of between 28 and 32 ℃ and at the speed of 140r/min for 24 hours, and then inoculated into a seed tank for expanded culture according to the process to prepare the yeast seed liquid, wherein the effective viable count of the seed liquid is required to be more than or equal to 109cfu/mL。
The preparation method of the bacillus licheniformis seed solution comprises the following steps: under the aseptic condition, LB culture medium is adopted to culture for 24 hours at the temperature of 28-32 ℃ to prepare liquid bacillus seed liquid, and the effective viable count of the seed liquid is required to be more than or equal to 109cfu/mL。
The invention provides a compound fermentation type shark cartilage powder health drink, which is rich in a plurality of functional active substances such as aminosugar, glycosaminoglycan, flavone, saponin and the like, has obvious immunity enhancement effect and obvious anti-tumor effect, is suitable for middle-aged and elderly consumer groups, is between health care wine and health care drink, and is a novel high-efficiency health drink.
The invention provides a compound fermentation type shark cartilage powder health drink, which has the following remarkable advantages compared with the prior art:
(1) the application scientifically combines the shark cartilage powder, the kudzu root and the dendrobium, and can fully utilize the growth promotion effect of Chinese herbal medicines such as the kudzu root and the dendrobium on microorganisms; the immunity enhancement effect of the shark cartilage powder health-care beverage is remarkably improved through the synergistic effect among the Chinese herbal medicines, and the experimental result shows that the synergistic effect among the Chinese herbal medicines is the main reason for the unexpected effect obtained by the compound microbial fermentation;
(2) the application utilizes good oxygen fungus (aspergillus niger, saccharomycetes, bacillus licheniformis) to ferment the Chinese herbal medicine raw materials jointly and draw shark cartilage powder, and the synergistic effect between the make full use of microorganism promotes the extraction rate and the external antioxidant capacity of shark cartilage powder, wherein: aspergillus niger can decompose cellulose at high temperature, and break the constraint of the cellulose on the escape of the functional active ingredients (flavone, polyphenol, etc.) of the shark cartilage powder; the yeast can generate various enzymes for degrading cell walls in the fermentation process, so that more active substances such as flavone and the like are released from the cell walls, and the yeast can generate the enzymes capable of releasing combined state phenolic acid, thereby being beneficial to improving the in-vitro antioxidant capacity of the shark cartilage powder; the bacillus licheniformis can kill pathogenic bacteria in fermentation liquor in the high-temperature fermentation process, has strong capabilities of producing amylase, protease and the like, and can degrade macromolecular starch and protein substances into microorganisms for utilization;
(3) the functionality of the shark cartilage powder health-care drink is obviously improved through aerobic fermentation and two-step anaerobic fermentation, and the technical effect of 1+1>2 is achieved.
(4) The liquid fermentation is adopted, so that the growth rate of the thalli is improved, the fermentation time is shortened, the production efficiency is improved, the fermentation process conditions are mild, the production cost is low, the large-scale production is easy, and the method plays an important role in improving the industrial economic benefit and large-scale popularization and application.
Detailed Description
The microbial activation methods in the following examples all adopt the following processes:
(1) the preparation method of the aspergillus niger seed liquid comprises the following steps: culturing for 24h at 35-40 deg.C in potato culture medium under aseptic condition to obtain Aspergillus niger seed liquid with effective viable count not less than 109cfu/mL。
(2) The preparation method of the yeast seed liquid comprises the following steps: under the aseptic operation condition, two rings of strains are picked from the inclined plane of the yeast YDP by using an inoculating ring, inoculated into a 500mL triangular flask containing 300mLPDA liquid culture medium, subjected to shaking culture at the temperature of between 28 and 32 ℃ and at the speed of 140r/min for 24 hours, and then inoculated into a seed tank for expanded culture according to the process to prepare the yeast seed liquid, wherein the effective viable count of the seed liquid is required to be more than or equal to 109cfu/mL。
(3) The preparation method of the bacillus licheniformis seed liquid comprises the following steps: under the aseptic condition, LB culture medium is adopted to culture for 24 hours at the temperature of 28-32 ℃ to prepare liquid bacillus seed liquid, and the effective viable count of the seed liquid is required to be more than or equal to 109cfu/mL。
Example 1
A compound fermentation type shark cartilage powder health beverage comprises the following specific process steps:
(1) preparing materials: the raw materials are accurately mixed according to the proportion of 100 parts of shark cartilage powder, 3 parts of kudzu root and 1 part of dendrobium
(2) Chinese herbal medicine pretreatment: removing impurities from the raw materials of the kudzuvine root and the dendrobium, crushing, sieving by a sieve of 80-150 meshes, adding purified water with the mass ratio of 10 times of that of the raw materials of the Chinese herbal medicines, and soaking for 30min at the temperature of 40-60 ℃; adding cellulase accounting for 2% of the mass ratio of the Chinese herbal medicine raw materials, extracting for 2min under the assistance of microwave, wherein the microwave power is 250-400W, and then standing for more than 30 min;
(3) preparing fermentation liquor: mixing the pulverized shark cartilage powder with the pretreated Chinese herbal medicine raw materials, adding 20 times of purified water, and then adding 0.15-0.20 mass percent (NH) of fermentation liquor4)2SO4、0.10～0.15% KH2PO4、0.1%MgSO4；
(4) And (3) sterilization: sterilizing at 121 deg.C for 15 min;
(5) aerobic fermentation: adding a compound microbial agent with the mass ratio of 2-3% of the enzymolysis liquid into the enzymolysis liquid after enzymolysis, and performing aerobic fermentation for 6-12 h at the temperature of 28-35 ℃; the composite microbial agent is a mixed bacterium of aspergillus niger, saccharomyces cerevisiae and bacillus licheniformis, and the inoculation amount ratio is 1:2: 1;
(6) anaerobic fermentation: after the aerobic fermentation is finished, adding 1-2% of lactobacillus powder, and carrying out anaerobic fermentation for 8-16 h at the temperature of 28-35 ℃;
(7) and (3) filtering: performing coarse filtration to remove fermentation thalli;
(8) centrifuging: centrifuging at 10000r/min, and separating supernatant;
(9) aging: ageing for 10-20 days at the temperature of 0-10 ℃ in order to promote the stability and clarification of the beverage;
(10) compounding: adding 1.5g/L xylitol, 2g/L isomaltose hypgather, 5g/L citric acid, 5g/L calcium lactate and 3g/L sucrose;
(11) membrane filtration: filtering the shark cartilage powder health beverage with microporous membrane with pore size of 0.15 μm;
(12) and (3) sterile filling: filling and sealing in an aseptic filling production line to obtain the compound fermented shark cartilage powder health-care beverage.
Example 2
A compound fermentation type shark cartilage powder health beverage comprises the following specific process steps:
(1) preparing materials: the raw materials are accurately mixed according to the proportion of 100 parts of shark cartilage powder, 5 parts of kudzu root and 2 parts of dendrobium
(2) Chinese herbal medicine pretreatment: removing impurities from the raw materials of the kudzuvine root and the dendrobium, crushing, sieving by a sieve of 80-150 meshes, adding purified water with the mass ratio of 10 times of that of the raw materials of the Chinese herbal medicines, and soaking for 30min at the temperature of 40-60 ℃; adding cellulase accounting for 2% of the mass ratio of the Chinese herbal medicine raw materials, extracting for 2min under the assistance of microwave, wherein the microwave power is 250-400W, and then standing for more than 30 min;
(3) preparing fermentation liquor: mixing the pulverized shark cartilage powder with the pretreated Chinese herbal medicine raw materials, adding 20 times of purified water, and then adding 0.15-0.20 mass percent (NH) of fermentation liquor4)2SO4、0.10～0.15% KH2PO4、0.1%MgSO4；
(4) And (3) sterilization: sterilizing at 121 deg.C for 15 min;
(5) aerobic fermentation: adding a compound microbial agent with the mass ratio of 2-3% of the enzymolysis liquid into the enzymolysis liquid after enzymolysis, and performing aerobic fermentation for 6-12 h at the temperature of 28-35 ℃; the composite microbial agent is a mixed bacterium of aspergillus niger, saccharomyces cerevisiae and bacillus licheniformis, and the inoculation amount ratio is 1:2: 1;
(6) anaerobic fermentation: after the aerobic fermentation is finished, adding 1-2% of lactobacillus powder, and carrying out anaerobic fermentation for 8-16 h at the temperature of 28-35 ℃;
(7) and (3) filtering: performing coarse filtration to remove fermentation thalli;
(8) centrifuging: centrifuging at 10000r/min, and separating supernatant;
(9) aging: ageing for 10-20 days at the temperature of 0-10 ℃ in order to promote the stability and clarification of the beverage;
(10) compounding: adding 1.5g/L xylitol, 2g/L isomaltose hypgather, 5g/L citric acid, 5g/L calcium lactate and 3g/L sucrose;
(11) membrane filtration: filtering the shark cartilage powder health beverage with microporous membrane with pore size of 0.15 μm;
(12) and (3) sterile filling: filling and sealing in an aseptic filling production line to obtain the compound fermented shark cartilage powder health-care beverage.
Example 3
A compound fermentation type shark cartilage powder health beverage comprises the following specific process steps:
(1) preparing materials: the raw materials are accurately mixed according to the proportion of 100 parts of shark cartilage powder, 5 parts of kudzu root and 1 part of dendrobium
(2) Chinese herbal medicine pretreatment: removing impurities from the raw materials of the kudzuvine root and the dendrobium, crushing, sieving by a sieve of 80-150 meshes, adding purified water with the mass ratio of 10 times of that of the raw materials of the Chinese herbal medicines, and soaking for 30min at the temperature of 40-60 ℃; adding cellulase accounting for 2% of the mass ratio of the Chinese herbal medicine raw materials, extracting for 2min under the assistance of microwave, wherein the microwave power is 250-400W, and then standing for more than 30 min;
(3) preparing fermentation liquor: will be provided withMixing the pulverized shark cartilage powder with the pretreated Chinese herbal medicine raw materials, adding 20 times of purified water, and then adding 0.15-0.20% (NH) of fermentation liquor by mass4)2SO4、0.10～0.15% KH2PO4、0.1%MgSO4；
(4) And (3) sterilization: sterilizing at 121 deg.C for 15 min;
(5) aerobic fermentation: adding a compound microbial agent with the mass ratio of 2-3% of the enzymolysis liquid into the enzymolysis liquid after enzymolysis, and performing aerobic fermentation for 6-12 h at the temperature of 28-35 ℃; the composite microbial agent is a mixed bacterium of aspergillus niger, saccharomyces cerevisiae and bacillus licheniformis, and the inoculation amount ratio is 1:2: 1;
(6) anaerobic fermentation: after the aerobic fermentation is finished, adding 1-2% of lactobacillus powder, and carrying out anaerobic fermentation for 8-16 h at the temperature of 28-35 ℃;
(7) and (3) filtering: performing coarse filtration to remove fermentation thalli;
(8) centrifuging: centrifuging at 10000r/min, and separating supernatant;
(9) aging: ageing for 10-20 days at the temperature of 0-10 ℃ in order to promote the stability and clarification of the beverage;
(10) compounding: adding 1.5g/L xylitol, 2g/L isomaltose hypgather, 5g/L citric acid, 5g/L calcium lactate and 3g/L sucrose;
(11) membrane filtration: filtering the shark cartilage powder health beverage with microporous membrane with pore size of 0.15 μm;
(12) and (3) sterile filling: filling and sealing in an aseptic filling production line to obtain the compound fermented shark cartilage powder health-care beverage.
Example 4 Effect of different Process conditions on the Immunopotentiating ability of shark cartilage powder health drink
1. Index measuring method
The health drink pair of the present application was tested for S according to the test method described in Zhangxiujuan et al, influence of shark cartilage powder on immune cell regulation function of S180 tumor-bearing mice180Influence of splenic lymphocyte proliferation function and phagocytic activity of phagocytic cells of tumor-bearing mice.
2. Test method and grouping
The mice were randomly divided into 10 groups, and 100 mg/kg was administered by gavage of health beverage-1The negative control group was given the same volume of physiological saline, 10 of each group. Well-growing S from day 7 of inoculation was aseptically aspirated180Ascites (viable cell count) in tumor-bearing mice>97%) of normal saline, diluted 1: 3, and inoculated subcutaneously in the right anterior axilla of mice at a rate of 0.2 mL/mouse. Administering after 24h of inoculation, 1 time/d, continuously administering for 10d, stopping administration, and collecting blood by eyeball on the next day;
the grouping method comprises the following steps: examples 1-3 groups of the health drink described in examples 1-3 were separately gavaged; control 1 group of the health drink of gavage example 1 without anaerobic fermentation; control 2 group of the health drink not fermented by aerobic fermentation in gastric lavage example 1; control 3 group of the health drink without anaerobic fermentation in gavage example 2; control 4 groups of the health-care drink which is not fermented by aerobic fermentation in the gastric lavage example 2; control 5 group of the health drink without anaerobic fermentation in gavage example 3; a control 6 group of health drinks not fermented by aerobic fermentation in gavage example 3; the test methods were all the same.
3. Test results
TABLE 2 influence of different process conditions on the nutritional and health-care ability of shark cartilage powder health-care beverage
| Test group | A570Value of | A490Value of |
| Negative control group | 0.2762 | 0.8137 |
| Example 1 test group | 0.5679 | 1.4928 |
| Example 2 test group | 0.4831 | 1.4015 |
| Example 3 test group | 0.4596 | 1.3724 |
| Control 1 group | 0.4673 | 1.1564 |
| Control 2 group | 0.3149 | 0.9742 |
| Control 3 group | 0.4964 | 1.2163 |
| Control 4 group | 0.3316 | 1.0012 |
| Control 5 group | 0.4830 | 1.1936 |
| Control 6 group | 0.3298 | 0.9895 |
The experiment result of the embodiment 4 shows that the compatibility of the Chinese herbal medicine raw materials and the synergistic effect of the fermentation strains have obvious influence on the nutrition and health care performance of the compound shark cartilage powder health care beverage.
The above embodiments are only used for illustrating the technical solution of the present invention, and not for limiting the same; although the invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof; such modifications and substitutions do not depart from the spirit and scope of the present invention as set forth in the appended claims.
Claims (7)
1. A compound fermentation type shark cartilage powder health drink is characterized in that kudzu root and dendrobium are added in the preparation process of the shark cartilage powder health drink, and the compatibility proportion is as follows: 100 parts of shark cartilage powder, 2-5 parts of kudzu root and 1-2 parts of dendrobium.
2. The compound fermented shark cartilage powder health drink as claimed in claim 1, wherein the Chinese herbal medicine raw materials are pre-extracted as follows: removing impurities from the raw materials of the kudzuvine root and the dendrobium, crushing, sieving by a sieve of 80-150 meshes, adding purified water with the mass ratio of 10 times of that of the raw materials of the Chinese herbal medicines, and soaking for 30min at the temperature of 40-60 ℃; adding cellulase accounting for 2% of the mass of the Chinese herbal medicine raw materials, extracting for 2min under the assistance of microwave with the microwave power of 250-400W, and standing for more than 30 min.
3. The compound fermented shark cartilage powder health drink as claimed in claim 1, which is prepared by aerobic fermentation and two-step anaerobic fermentation process, the specific process comprises:
(1) preparing fermentation liquor: mixing the pulverized shark cartilage powder with the pretreated Chinese herbal medicine raw materials, adding 20 times of purified water, and then adding 0.15-0.20 mass percent (NH) of fermentation liquor4)2SO4、0.10～0.15% KH2PO4、0.1%MgSO4；
(2) And (3) sterilization: sterilizing at 121 deg.C for 15 min;
(3) aerobic fermentation: adding a compound microbial agent with the mass ratio of 2-3% of the enzymolysis liquid into the enzymolysis liquid after enzymolysis, and performing aerobic fermentation for 6-12 h at the temperature of 28-35 ℃;
(4) anaerobic fermentation: after the aerobic fermentation is finished, adding 1-2% of lactobacillus powder, and carrying out anaerobic fermentation for 8-16 h at the temperature of 28-35 ℃;
(5) and (3) filtering: performing coarse filtration to remove fermentation thalli;
(6) centrifuging: centrifuging at 10000r/min, and separating supernatant;
(7) aging: ageing for 10-20 days at the temperature of 0-10 ℃ in order to promote the stability and clarification of the beverage;
(8) compounding: adding 1-1.5 g/L xylitol, 1-2 g/L isomaltose hypgather, 5-10 g/L citric acid, 5-10 g/L calcium lactate and 3-5 g/L sucrose;
(9) membrane filtration: filtering the shark cartilage powder health beverage with microporous membrane with pore size of 0.15 μm;
(10) and (3) sterile filling: filling and sealing in an aseptic filling production line to obtain the compound fermented shark cartilage powder health-care beverage.
4. The compound fermented shark cartilage powder health drink according to claim 3, wherein the compound microbial agent in step (3) is a mixture of Aspergillus niger, Saccharomyces cerevisiae and Bacillus licheniformis, and the inoculation amount ratio is 1:2: 1.
5. The compound fermented shark cartilage powder health drink according to claim 3, wherein the preparation method of the Aspergillus niger seed liquid comprises the following steps: culturing for 24h at 35-40 deg.C in potato culture medium under aseptic condition to obtain Aspergillus niger seed liquid with effective viable count not less than 109cfu/mL。
6. The compound fermented shark cartilage powder health drink according to claim 3, wherein the yeast seed liquid is prepared by the following steps: under aseptic conditions, from yeast YDP on slantInoculating two rings of strains from inoculating loop, inoculating into 500mL triangular flask containing 300mLPDA liquid culture medium, performing shaking culture at 28-32 deg.C for 24 hr at 140r/min, inoculating into seed tank, and performing amplification culture to obtain yeast seed solution with effective viable count of not less than 109cfu/mL。
7. The compound fermented shark cartilage powder health drink according to claim 3, wherein the preparation method of the bacillus licheniformis seed liquid comprises: under the aseptic condition, LB culture medium is adopted to culture for 24 hours at the temperature of 28-32 ℃ to prepare liquid bacillus seed liquid, and the effective viable count of the seed liquid is required to be more than or equal to 109cfu/mL。
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