CN1899056A - Mulbery leaf milk and its preparing method and use - Google Patents

Mulbery leaf milk and its preparing method and use Download PDF

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Publication number
CN1899056A
CN1899056A CN 200610045477 CN200610045477A CN1899056A CN 1899056 A CN1899056 A CN 1899056A CN 200610045477 CN200610045477 CN 200610045477 CN 200610045477 A CN200610045477 A CN 200610045477A CN 1899056 A CN1899056 A CN 1899056A
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mulberry leaf
milk
leaf
mulbery
effective component
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任青华
温晓燕
张博
林汝湘
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INSTITUTE OF BASIC MEDICINE SAMS
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INSTITUTE OF BASIC MEDICINE SAMS
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Abstract

The present invention discloses a kind of mulberry leaf milk compounded with effective component of mulberry leaf and milk. The preparation process of the mulberry leaf milk includes mixing medicine liquid containing effective component of mulberry leaf in 1 g/ml with fresh milk in the volume ratio of 1 to 20-28, blending, sterilizing, packing and sealing. The mulberry leaf milk has both the hypoglycemic function of mulberry leaf and all the advantages of milk. The present invention also discloses the application of the mulberry leaf milk as health beverage and medicinal beverage for preventing and treating diabetes.

Description

A kind of Mulbery leaf milk and preparation method thereof and application
Technical field
The present invention relates to the beverage product technical field, exactly relate to a kind of Mulbery leaf milk and preparation method thereof and application.
Background technology
Mulberry leaf are the Chinese medicine of the medicine-food two-purpose of health ministry approval, and are nontoxic, tasteless.That mulberry leaf have is hypoglycemic, reducing blood lipid, hypotensive, norcholesterol, antibiotic (its decoction experiment in vitro all has good antibacterial action to staphylococcus aureus, beta hemolytic streptococcus, corynebacterium diphtheriae, Escherichia coli, typhoid bacillus, shigella dysenteriae, Pseudomonas aeruginosa), antiviral, anti-oxidant, increase endurance, improve immunity, multiple pharmacological effect such as delay senility.Therefore, the development and application values of mulberry leaf is very high, and prospect is boundless.Scientists such as Moscow doctor Sha Dinuo of the former Soviet Union study in centenarian's the investigation of mystery and find that Ya Bazan mountain area villager has good health and a long life especially, year 100 the highest threescores of longevity, and the centenarian is everywhere as seen.They are healthy, and vigorous main cause is, the locality all over the mountains and plains all is a mulberry tree, villager's every day the early, middle and late white mulberry vein-juice of all drinking.Find in the 4th census of China's nineteen ninety that the old man more than 75 years old accounts for 2.84% in Pishan county 180,000 populations, the highest age 120 years old, 111 years old Ka Dieryi still can participate in the summer harvest.The investigation of the Chinese Academy of Sciences, medical college shows that this is that the local resident is with the cause of mulberry leaf for the normal drink of tea.
Mulberry leaf are developed the Japan that research starts from the eighties, and compatriots' the extensive approval and the great attention and the support energetically of government department have been obtained, the commodity that are developed to food and health products have tens kinds, as Mulberry-leaf Tea, mulberry leaf congee, mulberry leaf wine, mulberry leaf sugar, mulberry leaf noodles, mulberry leaf biscuit, mulberry leaf cake, mulberry leaf additive, mulberry leaf feed, fast food etc., its health-care effect and security are extensively confirmed.In recent years, China has accelerated paces to the developmental research of mulberry leaf, listing at present products such as Mulberry-leaf Tea, mulberry leaf wine, mulberry leaf pillow, mulberry leaf capsule for descend of blood fat, mulberry leaf blood sugar reduction oral liquid are arranged.Along with going deep into to mulberry leaf research, exploitation, more and more consider now the efficacy of drugs that it is unique and the health care of integration of drinking and medicinal herbs, modern medicine study shows, contain abundant alkaloid, glycosidase inhibitor and mulberry leaf polysaccharide in the mulberry leaf, can promote β emiocytosis insulin, insulin promotes cell synthetic and improve glycometabolism to utilization, the hepatic glycogen of sugar, reduces the peak value of food back blood sugar, and blood sugar is obviously descended.In view of the definite and significant hypoglycemic activity of mulberry leaf, develop a kind of diabetes of preventing and treat, and make the diabetic under the situation of not taking medicine or taking medicine less, involuntary make that blood sugar lowers not only have health-care effect, a but also drink of nutritious function, to satisfy allegro requirement of modern society and minimum living crowd's life and medical security, become important topic to mulberry leaf research, exploitation.
Natural nourishing healthy is acknowledged as the trend of Food Science development.
At present, yet there are no the hypoglycemic milk of the natural nourishing healthy that is used for the diabetic on the market.If the mulberry leaf effective component extracts with " multifunctional and nutritional storehouse " is added in the milk, the biological value of milk is attained a yet higher goal, but also increased the function of milk blood sugar reducing function, will bring great Gospel to the diabetic.
Summary of the invention
At the deficiencies in the prior art, the problem to be solved in the present invention provides a kind of Mulbery leaf milk and preparation method thereof and application, and health mulberry leaf drink kind is few, taste is complicated, mouthfeel is poor to improve, and the not high defective of nutritional labeling.
Mulbery leaf milk of the present invention is formed by mulberry leaf effective component extracts (water extraction+alcohol extracting) and plain chocolate compatibility, it is characterized in that described mulberry leaf effective component extracts is meant that the mulberry leaf active constituent content is 1~2 gram/every milliliter of soup; Described plain chocolate is dew fresh milk; And the compatibility volume ratio of mulberry leaf effective component extracts soup and dew fresh milk is 1: 20~28.
Wherein, described mulberry leaf effective component extracts is meant that the mulberry leaf active constituent content is 1 gram/every milliliter of soup; And the compatibility volume ratio of mulberry leaf effective component extracts soup and dew fresh milk is 1: 24.
In above-mentioned Mulbery leaf milk, described mulberry leaf effective component extracts is made by following method:
(1) gets the running water that mulberry leaf add its 8~12 times of volumes, soaked 30~50 minutes;
(2) under 80~100 ℃ of temperature, lixiviate 30 minutes is filtered, and gets filtrate I, and is standby;
(3) the mulberry leaf filter residue after will filtering with volumetric flowmeter, adds the running water of 7~9 times of amounts, extracts 15 minutes down at 80~100 ℃, filter, filtrate II;
(4) merging filtrate I and filtrate II concentrate under 100 ℃ of temperature, and being concentrated into the mulberry leaf active constituent content is 3~4 gram/every ml water solution;
(5) in above-mentioned concentrate, add 95% ethanol of 1 times of volume, under 0~4 ℃ of condition, static 48 hours, stir therebetween 2~3 times;
(6) filter the ethanol concentrate, go precipitation; Supernatant reclaims ethanol under 80 ℃ of temperature, making soup be concentrated into the mulberry leaf active constituent content is 1 gram/every milliliter of soup, promptly makes brownish red mulberry leaf effective component extracts.
Wherein, the volume ratio of described mulberry leaf of step (1) and running water is preferably 1: 10.
Wherein, step (2) or (3) described extraction temperature are preferably 100 ℃.
Wherein, the volume ratio of described mulberry leaf filter residue of step (3) and running water is preferably 1: 8.
The preparation method of Mulbery leaf milk of the present invention, it is characterized in that, with the mulberry leaf active constituent content is the mulberry leaf effective component extracts of 1 gram/every milliliter of soup, in volume ratio, ratio in 1: 20~28 and dew fresh milk compatibility, blend, sterilization becomes dress, sealing in the business demand mode, promptly makes described Mulbery leaf milk.
In the preparation method of above-mentioned Mulbery leaf milk, the compatibility volume ratio of described mulberry leaf effective component extracts and dew fresh milk preferably 1: 24.
Mulbery leaf milk of the present invention has had the hypoglycemic effect of mulberry leaf uniquenesses and all advantages of milk concurrently, and not only mouthfeel is the same with former milk with taste; do not have any change, good taste, color and luster are tempting, nutritious; and manufacture craft is simple, and cost is low, very easily in large-scale production and promotion and implementation.
Mulbery leaf milk of the present invention is as the application of the health beverages of prevention diabetes.
Mulbery leaf milk of the present invention is as the application of the medicine drink of treatment diabetes.
Mulberry leaf milk of the present invention except that effect with milk, also have hypoglycemic, reducing blood lipid, hypotensive, norcholesterol, antibiotic, antiviral, anti-oxidant, improve immunity, multiple biologically active and health care such as delay senility.The more important thing is that mulberry leaf milk can prevent and treat diabetes, make the diabetic under the situation of not taking medicine or taking medicine less, the involuntary blood sugar that makes lowers.
At present, whole world diabetic has reached 1.3 hundred million, and number is also increasing severely.In China, existing diabetic surpasses 3,000 ten thousand, and newly-increased every year on average patient 1,500,000.Complication such as the injury of kidney that diabetes cause, retinal hemorrhage, nervous lesion are directly threatening people's life.Therefore, the diabetic needs to take medicine everyday, needs to take medicine every year, has brought very big misery and heavy financial burden for individual and family.Mulberry leaf milk cost of the present invention has just increased 4-6 and has divided money on the basis of per generation milk cost, when industrialization is produced, and the mass purchase mulberry leaf, its cost also can reduce by 1/3.The mulberry leaf blood sugar reduction milk of drinking one bag of cost money more than 1 yuan every day has been eliminated the side effect that taking medicine has an injection brings simultaneously and has been alleviated the heavy financial burden of family than taking medicine and having an injection and reduced the cost more than ten times.
Following in recent years once to the health care summary of the research of mulberry leaf and its main active ingredient, function and milk:
Mulberry leaf are the Chinese medicine of the medicine-food two-purpose of health ministry approval, and are nontoxic, tasteless.Modern pharmacology studies show that, contains rich in protein, amino acid (18 kinds), vitamin (A, B in the mulberry leaf 1, B 2, C), necessary trace element and the several physiological active substances of human body such as cellulose, potassium, calcium, iron, zinc, be called by people in " multifunctional and nutritional storehouse ".Various nutritional labelings and content thereof see Table 1 in the mulberry leaf, and the physiological activator of tool health-care efficacy and content see Table 2 in the mulberry leaf.
Table 1: various nutritional labelings and content thereof in the mulberry leaf
Nutritional labeling in the mulberry leaf Amino acid (g/100g dry product) Vitamin (mg/100g dry product) Dietary fiber Mineral matter and trace element (mg/100g dry product)
Content Amino acid content is about 10.10 grams; Wherein essential amino acid is about 3.3 grams. Vitamin B1: 0.59 vitamin B2: 1.35 vitamin Cs: 31.6 carrotene: 7.4 retinols: 0.67 Dietary fiber: 52.9%, wherein Soluble Fiber 7.9% Ca 2699; K 3101; Zn 6.1; Fe 44.1; Na 39.9; P 238; Also contain trace elements such as a small amount of Cu, Mg, Mn in addition.
Table 2: the physiological activator of tool health-care efficacy and content in the mulberry leaf
The health mulberry leaf effect Hypoglycemic Reducing blood lipid Hypotensive Anti-oxidant, anti-ageing
Physiological activator Alkaloid Mulberry leaf polysaccharide Phytosterol GABA (GABA) Isoflavone compounds
Content (mg/1 00g) 1-DNJ (DNJ): about 100 Abundant Sitosterol: 46, stigmasterol: 3 About 220 Isoquercitrin is about 300, and quercitin is about 30, quercin about 100
Mulberry leaf effective component extracts of the present invention mainly refers to effective hypoglycemic compositions such as the mulberry leaf polysaccharide, alkaloid in the mulberry leaf, and the existing many tests of its hypoglycemic activity are confirmed, for example:
The Japan scientist once compared experiment with mulberry leaf powder and hypoglycemic drug glibenclamide capsule, and the male diabetes patient carries out clinical trial to 24 40-60 year.24 patients are divided into two groups at random, take hypoglycemic drug glibenclamide capsule (5mg/d) for one group, and another group is taken mulberry leaf powder (3g/d), and Time of Administration is 30 days.Blood plasma and urine to the patient after 30 days detect.The result shows, compares with hypoglycemic drug glibenclamide capsule, and mulberry leaf powder has tangible blood sugar reducing function, and the hypoglycemic rate is 37% (P<0.01).Simultaneously, 12% (P<0.01) that also descends of the cholesterol level in the blood.The effect that the mulberry leaf powders that the Japan scientist finds to take every day 3 grams are taken this hypoglycemic drug than dosage in accordance with regulations is better, and without any side effects.
No.1 Military Medical Univ. of PLA has also carried out clinical research to the white mulberry leaf blood-sugar-reducing effect, observational technique and result in natural plants application study center: 20 milliliters of mulberry leaf effective component extracts (10g)/patient/sky, took continuously 3 months.After 3 months the diabetic taken a blood sample on an empty stomach and detect, the result shows that the blood sugar decreasing effect total effective rate is 94.74%, wherein produce effects 50.88% (patient's blood sugar drops to normal level).
The achievement that mulberry leaf effective component extracts (effective hypoglycemic composition) hypoglycemic activity mechanism and correlative study obtain recently sees Table 3.
Table 3: the white mulberry leaf blood-sugar-reducing mechanism of action and correlative study achievement
Active ingredient Result of study
Folium Mori alkaloid (DNJ) When the coexistence of mulberry leaf beetle (platyxantha chinensis) alcohol extract was arranged, the sucrose absorption can be suppressed.Show that the mulberry leaf beetle (platyxantha chinensis) alcohol extract is inhibited to disaccharides catabolic enzyme activity.
The mulberry leaf water extract reduces rat peripheric venous blood glucose content, shows that the mulberry leaf water extract can suppress the activity of maltose and lactase.
Mulberry leaf powder raises to rat food back blood sugar obvious inhibitory action.
Folium Mori alkaloid hypoglycemic activity mechanism has two kinds: the one, suppressed the alpha-glucosidase activity, and the 2nd, promote insulin to discharge.
Folium Mori alkaloid fagomine Alkaloid fagomine can significantly increase the insulin content increase that glucose causes, thereby reduces blood glucose in diabetic mice.
Mulberry leaf polysaccharide The total polysaccharide TPM of mulberry leaf significantly improves insulin level reducing blood sugar simultaneously.
Milk is rich in two big anthropoid necessary and indispensable nutrients; One class is to keep the protein of human life, fat and sugar class material; One class is vitamin and the trace element that keeps physical health.According to textual criticism, in natural food, without any a kind of food can with comprehensively the comparing of the nutrient of milk.Therefore, milk is the irreplaceable human life's of any food nutrient source.Modern nutriology the man studies show that, the benefit of normal drink milk has: anti-cancer, anti-ageing, the hypnosis of promoting longevity, calm the nerves, strengthen immunity of organisms, promote children's intelligence to grow and grow etc.Nutritious, the old and the young of milk all are fit to be generally acknowledged.
The health care of milk is summarized as follows:
Casein in the ◎ milk contains 10% phosphorus, and the brain development that promotes the child is played crucial effects.
Containing potassium in the ◎ milk makes the arterial blood tube wall flow at great speed at blood to keep stable when high, make risk of stroke reduce half.
◎ milk can stop metallic lead poisonous in the absorption of human body food and cadmium, has slight function of detoxification.
Contain 5% lactose nearly in the ◎ milk, can promote the absorption of human body, strengthen enterocinesia, promote to drain calcium and iron.
◎ sour milk and defatted milk can strengthen the immune system function, stop tumour cell to increase.
Vitamin B2 in the ◎ milk can improve eyesight.
The ◎ drink milk also can prevent artery sclerosis.
◎ milk also has certain curative effect to gastric and duodenal ulcer.
Cruel propylhomoserin in the ◎ milk can promote happy hormone serum hormone growth.
Iron in the ◎ milk, copper and vitamin A have beautification function, make skin keep smooth and plentiful.
Magnesium in the ◎ milk can be alleviated heart and nervous system fatigue.
Zinc in the ◎ milk can promote that wound heals quickly.
Calcium in the ◎ milk can strengthen the bone tooth, makes the bone wasting disease reduce half, promotes infant and teen-age intellectual development and physiological development.
Protein, enzyme peptide, hormone etc. all have the activity of regulating body function in the ◎ milk, have prevention to infect, regulate body's immunity and antibacterial activity, can prevent multiple disease.
The specific embodiment
Embodiment 1
With the mulberry leaf active constituent content is the mulberry leaf effective component extracts of 1 gram/every milliliter of soup, and in volume ratio, in 1: 24 ratio and dew fresh milk compatibility, blend, sterilization becomes dress, sealing in the business demand mode, promptly makes described Mulbery leaf milk.
Wherein, described mulberry leaf effective component extracts is made by following method:
(1) gets mulberry leaf 50g, add the running water of its 10 times of volumes, soaked 30 minutes;
(2) under 100 ℃ of temperature, lixiviate 30 minutes is filtered, and gets filtrate I, and is standby;
(3) the mulberry leaf filter residue after will filtering with volumetric flowmeter, adds the running water of 8 times of amounts, extracts 15 minutes down at 100 ℃, filter, filtrate II;
(4) merging filtrate I and filtrate II concentrate under 100 ℃ of temperature, and being concentrated into the mulberry leaf active constituent content is 3 gram/every ml water solution;
(5) in above-mentioned concentrate, add 95% ethanol of 1 times of volume, under 0~4 ℃ of condition, static 48 hours, stir therebetween 2~3 times;
(6) filter the ethanol concentrate, go precipitation; Supernatant reclaims ethanol under 80 ℃ of temperature, making soup be concentrated into the mulberry leaf active constituent content is 1 gram/every milliliter of soup, promptly makes brownish red mulberry leaf effective component extracts.
Embodiment 2
With the mulberry leaf active constituent content is the mulberry leaf effective component extracts of 2 gram/every milliliter of soups, and in volume ratio, in 1: 28 ratio and dew fresh milk compatibility, blend, sterilization becomes dress, sealing in the business demand mode, promptly makes described Mulbery leaf milk.
Wherein, described mulberry leaf effective component extracts is made by following method:
(1) gets mulberry leaf 100g, add the running water of its 12 times of volumes, soaked 50 minutes;
(2) under 90 ℃ of temperature, lixiviate 30 minutes is filtered, and gets filtrate I, and is standby;
(3) the mulberry leaf filter residue after will filtering with volumetric flowmeter, adds the running water of 9 times of amounts, extracts 15 minutes down at 90 ℃, filter, filtrate II;
(4) merging filtrate I and filtrate II concentrate under 100 ℃ of temperature, and being concentrated into the mulberry leaf active constituent content is 4 gram/every ml water solution;
(5) in above-mentioned concentrate, add 95% ethanol of 1 times of volume, under 0~4 ℃ of condition, static 48 hours, stir therebetween 2~3 times;
(6) filter the ethanol concentrate, go precipitation; Supernatant reclaims ethanol under 80 ℃ of temperature, making soup be concentrated into the mulberry leaf active constituent content is 1 gram/every milliliter of soup, promptly makes brownish red mulberry leaf effective component extracts.
Embodiment 3
With the mulberry leaf active constituent content is the mulberry leaf effective component extracts of 1 gram/every milliliter of soup, and in volume ratio, in 1: 20 ratio and dew fresh milk compatibility, blend, sterilization becomes dress, sealing in the business demand mode, promptly makes described Mulbery leaf milk.
Wherein, described mulberry leaf effective component extracts is made by following method:
(1) gets mulberry leaf 50g, add the running water of its 8 times of volumes, soaked 40 minutes;
(2) under 80 ℃ of temperature, lixiviate 30 minutes is filtered, and gets filtrate I, and is standby;
(3) the mulberry leaf filter residue after will filtering with volumetric flowmeter, adds the running water of 7 times of amounts, extracts 15 minutes down at 80 ℃, filter, filtrate II;
(4) merging filtrate I and filtrate II concentrate under 100 ℃ of temperature, and being concentrated into the mulberry leaf active constituent content is 3.5 gram/every ml water solution;
(5) in above-mentioned concentrate, add 95% ethanol of 1 times of volume, under 4 ℃ of conditions, static 48 hours, stir therebetween 2~3 times;
(6) filter the ethanol concentrate, go precipitation; Supernatant reclaims ethanol under 80 ℃ of temperature, making soup be concentrated into the mulberry leaf active constituent content is 1 gram/every milliliter of soup, promptly makes brownish red mulberry leaf effective component extracts.

Claims (10)

1. a Mulbery leaf milk is formed by mulberry leaf effective component extracts and plain chocolate compatibility, it is characterized in that, described mulberry leaf effective component extracts is meant that the mulberry leaf active constituent content is 1~2 gram/every milliliter of soup; Described plain chocolate is dew fresh milk; And the compatibility volume ratio of mulberry leaf effective component extracts soup and dew fresh milk is 1: 20~28.
2. Mulbery leaf milk as claimed in claim 1 is characterized in that, described mulberry leaf effective component extracts is meant that the mulberry leaf active constituent content is 1 gram/every milliliter of soup; And the compatibility volume ratio of mulberry leaf effective component extracts soup and dew fresh milk is 1: 24.
3. Mulbery leaf milk as claimed in claim 1 or 2 is characterized in that, described mulberry leaf effective component extracts is made by following method:
(1) gets the running water that mulberry leaf add its 8~12 times of volumes, soaked 30~50 minutes;
(2) under 80~100 ℃ of temperature, lixiviate 30 minutes is filtered, and gets filtrate I, and is standby;
(3) the mulberry leaf filter residue after will filtering with volumetric flowmeter, adds the running water of 7~9 times of amounts, extracts 15 minutes down at 80~100 ℃, filter, filtrate II;
(4) merging filtrate I and filtrate II concentrate under 100 ℃ of temperature, and being concentrated into the mulberry leaf active constituent content is 3~4 gram/every ml water solution;
(5) in above-mentioned concentrate, add 95% ethanol of 1 times of volume, under 0~4 ℃ of condition, static 48 hours, stir therebetween 2~3 times;
(6) filter the ethanol concentrate, go precipitation; Supernatant reclaims ethanol under 80 ℃ of temperature, making soup be concentrated into the mulberry leaf active constituent content is 1 gram/every milliliter of soup, promptly makes brownish red mulberry leaf effective component extracts.
4. Mulbery leaf milk as claimed in claim 3 is characterized in that, the volume ratio of described mulberry leaf of step (1) and running water is 1: 10.
5. Mulbery leaf milk as claimed in claim 3 is characterized in that, step (2) or (3) described extraction temperature are 100 ℃.
6. Mulbery leaf milk as claimed in claim 3 is characterized in that, the volume ratio of described mulberry leaf filter residue of step (3) and running water is 1: 8.
7. the preparation method of claim 1 or 2 described Mulbery leaf milks, it is characterized in that, with the mulberry leaf active constituent content is the mulberry leaf effective component extracts of 1 gram/every milliliter of soup, in volume ratio, ratio in 1: 20~28 and dew fresh milk compatibility, blend, sterilization becomes dress, sealing in the business demand mode, promptly makes described Mulbery leaf milk.
8. as the preparation method of Mulbery leaf milk as described in the claim 7, it is characterized in that the compatibility volume ratio of described mulberry leaf effective component extracts and dew fresh milk is 1: 24.
9. claim 1 or 2 described Mulbery leaf milks are as the application of the health beverages of prevention diabetes.
10. claim 1 or 2 described Mulbery leaf milks are as the application of the medicine drink of treatment diabetes.
CN 200610045477 2006-07-13 2006-07-13 Mulbery leaf milk and its preparing method and use Pending CN1899056A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011524378A (en) * 2008-06-20 2011-09-01 湖南希▲爾▼天然▲薬▼▲業▼有限公司 Pharmaceutical composition for the treatment of diabetes
CN106900872A (en) * 2017-03-27 2017-06-30 华南农业大学 A kind of preparation method of the mulberry soya-bean milk containing gamma aminobutyric acid

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011524378A (en) * 2008-06-20 2011-09-01 湖南希▲爾▼天然▲薬▼▲業▼有限公司 Pharmaceutical composition for the treatment of diabetes
CN101606936B (en) * 2008-06-20 2011-12-28 中南大学 Medicinal composition for treating diabetes
CN106900872A (en) * 2017-03-27 2017-06-30 华南农业大学 A kind of preparation method of the mulberry soya-bean milk containing gamma aminobutyric acid
CN106900872B (en) * 2017-03-27 2020-04-14 华南农业大学 Preparation method of mulberry milk containing gamma-aminobutyric acid

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