CN105125972A - Chinese herbal preparation for improving intestinal microecology and repairing intestinal mucosa injuries of piglets - Google Patents
Chinese herbal preparation for improving intestinal microecology and repairing intestinal mucosa injuries of piglets Download PDFInfo
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Abstract
The invention discloses a Chinese herbal preparation for improving intestinal microecology and repairing intestinal mucosa injuries of piglets. The Chinese herbal preparation comprises the following raw materials in parts by mass: 1 part of radix codonopsitis, 1 part of astragalus, 1.3-1.4 parts of roasted rhizoma atractylodis macrocephalae, 1.3-1.5 parts of rhizoma discoreae, 1 part of semen myristicae, 1 part of cortex mangnoliae officinalis, 1 part of pericarpium citri reticulatae, 1 part of rhizoma atractylodis, 1 part of caramel malt, 1 part of charred fructus crataegi, 1 part of charred medicated leaven, 1 part of radix puerariae and 1 part of radix glycyrrhizae. The Chinese herbal preparation is applied in the way that each piglet takes 10 g of the Chinese herbal preparation, granules of the Chinese herbal preparation are dissolved by 5 times of warm boiled water at the temperature of 80-90 DEG C, uniform stirring is performed for sufficient dissolution, and then the Chinese herbal preparation can be taken.
Description
Technical field
The present invention relates to veterinary drug technical field, be specifically related to a kind ofly prevent and treat piglet diarrhea and repair the Chinese medicine preparation of intestine of young pigs Tiny ecosystem and intestinal mucosa injury, and the preparation method of said preparation and using method.
Background technology
Diarrhoea causes the key factor of piglet high mortality, and piglet adopts early weaned technology, can improve fertility of sow, but early-weaning again can because piglet digestive system is unsound, replacing feedstuff stress wait and occur early weaning piglet syndrome excessively.Show as appetite to reduce, dyspepsia, efficiency of feed utilization is low, and premunition declines, and occur the symptoms such as diarrhoea, diarrhea of weaned piglets is the typical multiple diseases of one under intensive manufacture condition.According to investigations, in China, diarrhea of weaned piglets rate is up to 50% ~ 60%, and mortality rate is up to 20%, occurred frequently wean 10 ~ 5 days, this disease is popular wide, falls ill all the year round, and the piglet of recovering also often causes poor growth because of the imbalance of intestinal microecology and the damage of intestinal mucosa, the price of deed reduces, finally cause growth inhibited, even occur cad pig or death, cause great economic loss to pig industry.
Intestinal microecology is complicated and diversified microbiota in animal intestinal, this fauna to the growth of host and health significant.The composition of Tiny ecosystem flora in intestinal piglet and change are directly affected to health and the physiological function of pig, microorganism in intestine of young pigs forms the microorganism species being applicable to pig gradually in Piglet Development process, and reach optimum microecological balance state gradually, wherein bacillus bifidus and lactobacillus have comparative advantage.Probiotic bacteria is a class and host's Mutualism, is conducive to the antibacterial of raiseeing main health, once be destroyed or quantity minimizing, will cause disease, make piglet occur diarrhoea, dehydration, weight loss, the symptoms such as fertility performance reduction.The health and lives activity of intestine of young pigs probiotic bacteria to piglet has a very important role.Its Main Function is: (1) strengthens digestive function: the hydrolytic enzyme that probiotic bacteria produces, protein, fat and carbohydrate in the energy such as fermentation enzyme and respiratory enzyme hydrolysate feed, lactose fermenters generates the lactic acid being conducive to absorbing, and increases the content of solubility calcium, phosphorus and some vitamin B group.Probiotic bacteria and metabolite thereof can promote the secretion of body digestive enzyme and the wriggling of intestinal, digesting and assimilating of further promotion food, the utilization rate of feedstuff is greatly improved, and the probiotic bacterias such as lactobacillus participate in body metabolism, promote that piglet growth improves fertility performance.(2) kill harmful pathogen: the metabolite low molecular acid antibacterial peptide etc. that probiotic bacteria produces effectively can kill pathogenic microorganism, its anaerobic environment of breeding and producing, makes the growth of aerobic pathogenic bacterium and breeding receive suppression, thus reduces the generation of disease.
Animal intestinal mucosa structure is divided into mucous layer from inside to outside, Submucosa, muscle layer and theca externa.The normal configuration of intestinal mucosa and function be nutrient substance by the thickness of the basic guarantee of abundant peptic ulcer bleeding, particularly intestinal mucosa, villus length, it is the important indicator weighing small intestinal digestive and absorptive functions that Crypt depth and fuzzy surface amass.Normal intestinal mucosa structural integrity, well arranged, intestinal villus is complete, has branch as seen, the striated structure straight uniform of intestinal mucosa epithelial surface, lamina propria clear in structure, and enteraden is flourishing.Wherein intestinal villus length and cell quantity are significant correlation, and when fine hair shortens, ripe villus cell reduces, and reduces the absorbability of nutrient.Mucous layer is not only digestion in addition, absorbs the place of nutrient substance and secretion, but also has important barrier and immunologic function, and gut barrier is primarily of mechanical barrier, and biological barrier and immunologic barrier form.Described mechanical barrier is by Intestinal epithelial cells and compact siro spinning technology thereof; and the mucus of Intestinal epithelial cells secretion forms a kind of elastic gel layer and is covered in intestinal mucosa surface; form the physical barriers that one harmful microorganism can not freely be gone beyond, rete malpighii can also protect intestinal mucosa from chemistry and mechanical injuries simultaneously.Described biological barrier and Tiny ecosystem barrier.Studies have found that in the slime layer on intestinal mucosa surface the specific receptor having and specialize in anaerobe and probiotic bacteria (as lactobacillus and bacillus bifidus) and combine, these probioticss are embedded between mucosal epithelial cells in an orderly manner, playing the physiological action that it is extremely strong. its Main Function is as follows: Nutrition: the metabolite of flora is except supply antibacterial self utilizes, and a part is absorbed by host.As intestinal microbial population can synthesize several amino acids and vitamin, there is Nutrition.Immunization: the antigenic stimulus of normal flora can make host produce immunity, activates the activity of host autoimmune system.Ant agonism: Ant agonism has two kinds, one is that some resident bacterium can produce antibiotic substance and bacteriocidin, attacks bacterium outside can suppressing and kill; Also have one to be occupancy protection, normal flora captures position by being closely combined with mucosal epithelial cells, due to very large in these position quantity, has the advantage in nutrient competition, thus reaches the effect suppressing harmful pathogenic bacteria.And the pH value changed by the acidic materials that own metabolism produces in environment, thus suppress the growth of foreign bacteria.Described immunologic barrier: the immunity of intestinal mucosa depends primarily on the quantity of lymphocyte between intestinal mucosa epithelium and lamina propria endolymph cell.Also have on some intestinal mucosas and have local immunity organ, as cecal tonsil.Therefore the integrity of intestinal mucosa and functional status to the health status safeguarding whole body and there is the life quality of good general function state and disease prognosis, the recovery of fertility performance all has very important effect, so, carry out Chinese medicine further and improve animal intestinal micro-ecology, repair intestinal mucosa injury and have very important significance.
Ablactational baby pig intestinal mucosa system is not well established, mucosa-immune is low, is easy to bring out various gastroenteropathy, causes diarrhoea, and with often causing piglet intestinal mucosa destruction and Intestinal flora disorder etc. during antibiotic therapy, carry out heavy economic losses to Pig Industry industrial belt.The integrity of intestinal mucosa and functional status are to the health status safeguarding whole body and there is the life quality of good general function state and disease prognosis, the recovery of fertility performance all has very important effect.Chinese medicine has the feature of effecting a permanent cure of taking stopgap measures and fundamentally can remove the cause of disease and reach the inaccessiable therapeutic effect of antibiotic, and can correct the untoward reaction such as the drug resistance caused because of abuse of antibiotics.So, carry out Chinese medicine further and improve animal intestinal micro-ecology, repair intestinal mucosa injury and have very important significance.
At present, for the reparation of intestinal mucosa injury, adopt probiotic bacteria, nutrient substance, the alimentation factor and antibacterial peptide etc. more.But because the higher and cost of its technical requirement fails greatly universal and application.
Summary of the invention
For the deficiency that prior art exists, the invention provides a kind of Chinese medicine preparation repairing intestine of young pigs Tiny ecosystem and intestinal mucosa injury, this Chinese medicine preparation can effectively reduce piglet diarrhea frequency, and the microecological environment that can effectively improve in intestine of young pigs, significantly improve the quantity (CFU/g) of probiotic lactic acid bacillus and bacillus bifidus in intestine of young pigs, to pathogenic bacterium escherichia coli in intestinal, there is obvious inhibitory action.Inflammatory cell infiltration in remarkable reduction intestinal mucosa, microvillus marshalling; The organelle of impaired lymphocyte (LELs), goblet cell (GSs) recovers normal, and the nutrient digestibility of each nutritional labeling all increases, to organic substance, available phosphorus (P), calcium (Ca), crude protein (CP), crude fibre (CF), the digestibility of crude fat (EE) all increases.Effective raising daily gain and growth performance; And effectively can improve the quantity of diarrhoea Small Intestine of Piglets mucosal immune cells, improve immunity of organisms, for Pig Industry field provides theoretical foundation using Chinese medicine substitute antibiotics as additive, and then promote that piglet diarrhea is fully recovered and improves the growth performance more.Chinese medicine preparation processing cost provided by the invention is low, and using method is environmental protection simply again, has significant economic benefit and social benefit.
The present invention is achieved by the following technical solutions:
A kind of Chinese medicine preparation repairing intestine of young pigs Tiny ecosystem and intestinal mucosa injury of the present invention is made up of the raw material of following mass parts: Radix Codonopsis 1, the Radix Astragali 1, Rhizoma Atractylodis Macrocephalae (parched) 1.3-1.4, Rhizoma dioscoreae 1.3-1.5, Semen Myristicae 1, Cortex Magnoliae Officinalis 1, Pericarpium Citri Reticulatae 1, Rhizoma Atractylodis 1, Fructus Hordei Germinatus (parched to brown) 1, Fructus Crataegi (parched to brown) 1, Massa Medicata Fermentata (parched to brown) 1, Radix Puerariae 1, Radix Glycyrrhizae 1.
A kind of preparation method of repairing the Chinese medicine preparation of intestine of young pigs Tiny ecosystem and intestinal mucosa injury of the present invention comprises the steps:
(1) take Radix Codonopsis 1 part, the Radix Astragali 1 part, Rhizoma Atractylodis Macrocephalae (parched) 1.3-1.4 part, Rhizoma dioscoreae 1.3-1.5 part, Semen Myristicae 1 part, Cortex Magnoliae Officinalis 1 part, Pericarpium Citri Reticulatae 1 part, Rhizoma Atractylodis 1 part, Fructus Hordei Germinatus (parched to brown) 1 part, Fructus Crataegi (parched to brown) 1 part, Massa Medicata Fermentata (parched to brown) 1 part, Radix Puerariae 1 part, 1 part, Radix Glycyrrhizae, after mixing, be crushed to 10-20 order;
(2) get the medicinal powder immersion 2-4h that (1) prepares by whole quality of medicinal material 5-6 clear water doubly, be then heated to boil, continue 20-30min with slow fire, then solid-liquid separation;
(3) add whole quality of medicinal material 3-5 clear water doubly in the solid phase be separated in (2), be heated to boil, continue 20-30min with slow fire, then solid-liquid separation;
(4) merge (2), (3) twice filtrate, be evaporated to the thick paste that proportion is 1.30-1.35, add the dextrin of thick paste quality 50%-55%, make 60 object granules with granulator, pack.
A kind of using method of repairing the Chinese medicine preparation of intestine of young pigs Tiny ecosystem and intestinal mucosa injury of the present invention is: take 10g every day by every piglet, by granule 5 times of 80 DEG C-90 DEG C warm water impulsive motions, stirs after fully dissolving and takes.
Pharmacology mechanism of the present invention:
A kind of Chinese medicine preparation repairing intestine of young pigs Tiny ecosystem and intestinal mucosa injury of the present invention with spleen invigorating QI invigorating, warming middle-JIAO to dispel cold, go long-pendingly to help digestion, clearing away heat and eliminating dampness for Therapeutic Principle, in formula, Radix Codonopsis, the Radix Astragali, Rhizoma Atractylodis Macrocephalae (parched), Rhizoma dioscoreae spleen invigorating QI invigorating, be monarch drug; Semen Myristicae, Cortex Magnoliae Officinalis, Pericarpium Citri Reticulatae, Rhizoma Atractylodis warming middle-JIAO to dispel cold are ministerial drug; Fructus Hordei Germinatus (parched), Fructus Crataegi (parched), Massa Medicata Fermentata preparata go to amass and help digestion for adjuvant drug; Radix Puerariae has clearing away heat and eliminating dampness, anti-infective effect, coordinates Radix Glycyrrhizae invigorating the spleen and replenishing QI, coordinating the actions of various ingredients in a prescription, and can the toxicity, strong of cushion, for making medicine.All medicines share to meet and dialectically treat rule, can not so lose that, treatment of deficiency-syndrome by reinforcement can be reached, cold person's using warming therapy, food stagnation disappear it, clearing moist heat it, chronic diarrhea rise it, sliding then astringent or styptic treatment for spontaneous sweating therapeutic effect and regulate the effect of metabolism.Wherein, Radix Codonopsis can the QI-insufficiency of tonifying the spleen and stomach, strengthening the spleen and stomach.The Radix Astragali can be used for QI invigorating and promotes spleen function, also expansible blood vessel, improves blood circulation and nutriture.Rhizoma Atractylodis Macrocephalae (parched) invigorating the spleen and replenishing QI.Rhizoma dioscoreae spleen reinforcing nourishing the stomach.Semen Myristicae, Cortex Magnoliae Officinalis, Pericarpium Citri Reticulatae, Rhizoma Atractylodis warming spleen and stomach for dispelling cold, recuperating depleted YANG is promoted blood circulation.Cure mainly warming middle-JIAO astringing intestine to stop diarrhea, promote qi circulation digestion promoting, do not disappear for diarrhea of deficiency type, cold-dysentery, abdominal distention, lack of appetite vomiting, dyspepsia.JIAOSANXIAN (Fructus Crataegi, Fructus Hordei Germinatus, Massa Medicata Fermentata) is made up of Fructus Hordei Germinatus (parched to brown), Fructus Crataegi (parched to brown), Massa Medicata Fermentata (parched to brown) three taste medicine, there is the effect that good removing food stagnancyization is stagnant, Fructus Hordei Germinatus (parched to brown) has the effect of good digestible starch group food, Fructus Crataegi (parched to brown) is good at treating meat or greasy too much caused dyspepsia, Massa Medicata Fermentata (parched to brown) contains amylase, yeast and abundant vitamin B group, be then beneficial to digestion rice and flour food, three medicines share, can strengthen digestive function significantly, the carbonization of JIAOSANXIAN (Fructus Crataegi, Fructus Hordei Germinatus, Massa Medicata Fermentata) also can play the effect of astringing to arrest diarrhea.Radix Puerariae has clearing away heat and eliminating dampness, anti-infective effect.Radix Glycyrrhizae invigorating the spleen and replenishing QI, coordinating the actions of various ingredients in a prescription, and can the toxicity, strong of cushion, for making medicine.
The mechanism of action of the present invention is as follows: Radix Codonopsis can improve intestinal blood flow, prevents bacterial translocation from intestine.The expansible blood vessel of the Radix Astragali; improve blood circulation and nutriture, after can making Gut ischmia-reperfusion, blood plasma and small intestine's blood plasma superoxidase (SOD) activity raise, and protection of intestinal mucosal barrier cells apoptotic index obviously reduces; thus to ischemia, gut barrier damage plays a protective role.The Rhizoma Atractylodis Macrocephalae can reduce the intensity of inflammatory reaction, and protection intestinal mucosa mechanical barrier, lowers intestinal permeability and improve mucosa-immune function.The puerarin of Radix Puerariae can reduce the permeability of intestinal mucosa and gut cell membrane; alleviate gut barrier damage; play the effect of protection protection of intestinal mucosal barrier cells; it can promote a large amount of hypertrophy of goblet cell in intestinal mucosa simultaneously; and goblet cell can secrete a large amount of mucus in intestinal mucosa surface, it can stop enteric cavity endotoxin, antibacterial contacts with epithelial cell and damages intestinal mucosa surface.
chinese medicine preparation of the present invention is prevented and treated piglet diarrhea and is repaired the efficacy test of intestinal mucosa injury
1, Chinese medicine preparation of the present invention control piglet diarrhea, the test that improves intestinal microecology, improve nutrient digestibility and growth performance
1.1 test method
In order to study the impact of Chinese medicine preparation of the present invention on diarrhea of weaned piglets, intestinal microecology, nutrient digestibility and growth performance, start to JIUYUE 11 end of day on May 13rd, 2014, test site is carried out on the grand sharp pig farm in fanshi sand town, suburb, Taiyuan.Select artificial challenge's escherichia coli to cause the DLY piglet 54 of 28 age in days wean of diarrhoea, stochastic averagina is divided into 3 groups, often organizes 18, often organizes 3 repetitions, each repetition 6.I group be antibiotic therapy group, II group be Chinese medicine preparation treatment group of the present invention, III group be need not the blank group of any Drug therapy.Respectively at after medication when the 14th day, measure diarrhea frequency, and often organize and eachly repeat to randomly draw 1 and butcher, aseptic collection jejunum, ileum and caecum intestinal contents, utilize colony counting method to measure each intestinal segment escherichia coli, bacillus bifidus and lactobacillus quantity.Adopt above-mentioned butcher after I group, II group remaining 15 piglets, and set up sodium selenite 15 to do blank, after continuing to raise 14d, carry out digestion trial, adopt endogenous indicator method, experimental period is 4d, receive excrement every day 3 times, each about 100g, loads in sealing bag and seals, preserve in-20 DEG C of refrigerators.During test, excrement sample quick-thawing, in units of repeating by the mixing of receipts excrement sample, add 10% tartaric acid of 1/4 fresh excrement quality, in 65 DEG C of removings just moisture, pulverize (crossing 0.42mm sieve) bottling, each nutrient content to be measured.Analyze the various nutritional labelings in feedstuff and excrement sample according to " animal nutrition experiment textbook ", calculate each group of nutrient digestibility.And before testing and when treating latter 2 weeks and 4 weekend, each group of pig is weighed, detect the growth performance of each group of piglet, and adopt the ANOVA program in spss18.0 software to carry out variance analysis and Deng Kenshi (Duncan) multiple comparisons, data represent with (x ± s), p<0.05 is for there being statistical significance, p<0.01 is for there being remarkable statistical significance, and quantitative parameter compares uses one-sided t-test.
1.2 result of the test
1.2.1 Chinese medicine preparation of the present invention is on the impact of early-weaned piglets diarrhoea situation
Result of the test is in table 1.
Result of the test shows, persistent period of Chinese medicine preparation treatment group piglet diarrhea of the present invention be 2.15d and antibiotic group 2.38d all than the 3.03d pole of matched group significantly shorten piglet diarrhea persistent period (
p<0.01), and the piglet diarrhea time 2.15d of Chinese medicine preparation treatment group of the present invention compared with the piglet diarrhea time 2.38d of antibiotic group no significant difference (
p>0.05); The diarrhea frequency of Chinese medicine preparation treatment group of the present invention is all remarkable 21.01(lower than matched group of diarrhea frequency 14.70% of 12.85% and antibiotic group
p<0.05), the diarrhea frequency 12.85% of Chinese medicine preparation treatment group of the present invention compare with the diarrhea frequency 14.70% of antibiotic group no significant difference (
p>0.05); Chinese drug-treated group Scours index is all remarkable 5.56(P<0.05 lower than matched group of Scours index 3.83 of 3.08 and antibiotic group), and Chinese drug-treated group compares no significant difference (P>0.05) with the Scours index of antibiotic group.Therefore, this Chinese medicine preparation of the present invention has the anti-CaM antibody same compared with antibiotics.
1.2.2 Chinese medicine preparation of the present invention is on the impact of escherichia coli in ablactational baby pig intestinal
Result of the test is in table 2.
From table 2 meansigma methods, in antibiotic therapy group intestinal, in the quantity 6.29CFU/g of escherichia coli and Chinese medicine preparation treatment group intestinal of the present invention, escherichia coli quantity 6.44CFU/g is all extremely remarkable in the 7.52CFU/g(P<0.01 in matched group), Chinese medicine preparation treatment group of the present invention is no significant difference (P>0.05) compared with antibiotic therapy group.Result illustrates, Chinese medicine preparation of the present invention has the effect of roughly the same antibiotic suppression escherichia coli.
1.2.3 Chinese medicine preparation of the present invention is on the impact of lactobacillus in ablactational baby pig intestinal
Result of the test is in table 3.
From meansigma methods, in Chinese medicine preparation treatment group intestinal contents of the present invention, the quantity of lactobacillus is 8.53(CFU/g), extremely significantly be greater than the 7.25(CFU/g of antibiotic therapy group) (P<0.01), and be significantly greater than the 8.09(CFU/g of blank group) (P<0.05); In antibiotic therapy group intestinal contents, the quantity of lactobacillus is 7.25(CFU/g) be significantly less than 8.09(CFU/g in blank group) (P<0.05); Result shows, compared with matched group, and the quantity of lactobacillus in Chinese medicine preparation treatment group energy available protecting ablactational baby pig intestinal contents of the present invention, and antibiotic therapy group significantly reduces the quantity of probiotics lactobacillus.
1.2.4 Chinese medicine preparation of the present invention is on the impact of bacillus bifidus in ablactational baby pig intestinal
Result of the test is in table 4.
From meansigma methods, the quantity of the bacillus bifidus of Chinese medicine preparation treatment group of the present invention is 7.51(CFU/g) be extremely significantly greater than the 6.57(CFU/g of antibiotic therapy group) (P<0.01), and be significantly greater than the 7.28(CFU/g of blank group) (P<0.05), the quantity of antibiotic therapy group bacillus bifidus is 6.57(CFU/g) be significantly less than the 7.28(CFU/g of blank group) (P<0.05); Result shows, compared with matched group, and the quantity of bacillus bifidus in Chinese medicine preparation energy available protecting ablactational baby pig intestinal contents of the present invention, and antibiotic considerably reduces the quantity of probiotics bacillus bifidus.
1.2.5 Chinese medicine preparation drug sensitivity testing in vitro of the present invention
Result of the test is in table 5.
Result shows: Chinese medicine preparation of the present invention and antibiotic are 30mm and 27mm to the antibacterial circle diameter of escherichia coli respectively, all high responsive, proves that Chinese medicine preparation of the present invention has the bacteriostasis of antibiotic sample to escherichia coli.Antibiotic is 26 to lactobacillus antibacterial circle diameter simultaneously, be 27 to bacillus bifidus antibacterial circle diameter, all have high responsive bacteriostasis, Chinese medicine preparation of the present invention is then 6 to the antibacterial circle diameter of lactobacillus, be 7 to the antibacterial circle diameter of bacillus bifidus, all belong to antibacterial insensitive.Result shows, such antibiotic also has high responsive bacteriostasis to probiotics lactobacillus and bacillus bifidus, and Chinese medicine preparation of the present invention to lactobacillus and bacillus bifidus antibacterial insensitive.
1.2.6 Chinese medicine preparation of the present invention is on the impact of diarrhoea Small Intestine of Piglets mucosa nutrient digestibility
Result of the test is in table 6.
Add the impact on Main Nutrients apparent digestibility (%) after Chinese medicine preparation of the present invention and antibiotic in diet: as shown in table 1, sodium selenite group, antibiotic group and of the present invention group of digestibility difference to organic substance, phosphorus are not significantly (P>0.05); Of the present invention group is significantly higher than antibiotic group (P<0.05) to the digestibility of crude protein, crude fat, crude fibre, calcium (Ca), and with sodium selenite group difference not significantly (P>0.05).Result of the test shows, adds Chinese medicine preparation of the present invention and all can improve the apparent digestibility of piglet to crude protein, crude fat, crude fibre, calcium compared with antibiotic in diet.Improve digestibility and the utilization rate of nutrient substance, the metabolism of enhancing body, blood circulation promoting, improve the speed of growth of piglet.
1.2.7 Chinese medicine preparation of the present invention is on the impact of Growth Performance of Weaning Piglets
Result of the test is in table 7.
Result shows: Chinese medicine preparation treatment group piglet of the present invention body weight in time treating latter 2 weeks and 4 weekend increases very fast, growth performance is all significantly higher than antibiotic therapy group (P<0.05), and being significantly higher than blank group (P<0.05), the growth performance of antibiotic therapy group and blank group difference are not significantly (P>0.05).Result shows, Chinese medicine preparation of the present invention effectively can improve the growth performance of piglet.
2, Chinese medicine preparation of the present invention repairs the efficacy test of piglet intestinal mucosa injury
2.1 Chinese medicine preparation of the present invention repair the efficacy test of Small Intestine of Piglets mucous membrane tissue pathologic structure
2.1.1 the whole test of test method from 1 day August in 2014 to JIUYUE 17 end of day, this test is selected and to be bought from the grand sharp pig farm in fanshi sand town, suburb, Taiyuan, the DLY piglet that artificial challenge's escherichia coli causes the 28d of diarrhoea to wean 36 (28 ± 1) d, and it is consistent according to parity, kind is identical, body weight quality is close, the principle of male and female half and half is divided into 2 groups at random, often organize 18, often organize 3 repetitions, each repetition 6, I group is antibiotic therapy group, II group is Chinese native medicine compound prescription pellet agent treatment group, antibiotic therapy group adds lincomycin hydrochloride 2.0ml/ head drinking-water in drinking-water, 2 times/d, continuous 5d.Chinese native medicine compound prescription pellet agent group adds 5.0g/ head drinking-water in drinking-water, 2 times/d, continuous 5d.Respectively at after medication when 14 days, often group is randomly drawed 3 (each repetition randomly draws 1), carotid artery sacrificed by exsanguination, and aseptic collection small intestinal mucosa is dissected in abdominal cavity, after observing pathological change, by paraffin section and HE dyeing observation small intestinal mucosa Histopathologic changes.
Paraffin section and HE colouring method as follows:
(1) fixing: asepticly to take duodenum, ileum 5cm intestinal tissue block, after normal saline flushing, in 4% formalin fixative, fix 24h.
(2) dewater, transparent: piece of tissue respectively to be dewatered 2h through graded ethanol (50%-70%-85%-95%-dehydrated alcohol), then puts into dimethylbenzene successively: anhydrous alcohol (1:1) and the transparent each 1.5h of dimethylbenzene B, D;
(3) waxdip and Bao Li: at each waxdip 2h of waxdip box B, D, carries out embedding (embedding is wanted rapidly) with 60 DEG C of paraffin subsequently;
(4) cut into slices: be cut into rotary microtome the section 5 that continuous whole thickness is 5 μm;
(5) open up sheet and bonding die: extend piece in 45 DEG C of water-baths, microscope slide carries out paster (microscope slide bonding die agent APES process), dry more than 24h for 37 DEG C, room temperature is preserved, to be dyed.
(5) dye: paraffin section is through the dewaxing of dimethylbenzene routine, dimethylbenzene B, the each 15min of dimethylbenzene D, dimethylbenzene: anhydrous alcohol (1:1), anhydrous alcohol and graded ethanol at different levels (95%, 85%, 70%, 50%) each 2min, come downwards to water, to dye about 25min with hematoxylin solution, with distillation washing 2min, color separation in 0.5% hydrochloride alcohol solution (70 ~ 80s), be as the criterion with microscopy, through tap water running water oil blackeite 15min, again with after distillation washing, 95% ethanol (each 3min) is gone upward to through graded ethanol, enter 0.5% Yihong alcohol liquid dyeing 10min, enter 95% ethanol B more successively, the each 3min of 95% ethanol D() and anhydrous alcohol B, anhydrous alcohol D dewaters (each 5min), through dimethylbenzene B and dimethylbenzene D transparent (each 8min), neutral gum mounting.
2.1.2 result of the test
2.1.2.1 small bowel inflammatory cell infiltration degree
As shown in Figure 1, the inflammatory cell infiltration based on lymphocyte in the chorioepithelium of antibiotic therapy Small Intestine of Piglets wall tissue is obvious, and inflammatory cell has reached lamina propria and Submucosa, illustrates that inflammation scope is larger.As shown in Figure 2, in the chorioepithelium for the treatment of by Chinese herbs group Small Intestine of Piglets wall tissue of the present invention, there are a little lymphocyte and other inflammatory cell infiltrations, at lamina propria and Submucosa there are no lymphocytic infiltration.
2.1.2.2 small intestinal mucosa epithelial cell shedding degree
As shown in Figure 3, the degeneration of antibiotic therapy group Small Intestine of Piglets mucosal epithelial cells, downright bad, have the epithelial cell and erythrocyte that come off in enteric cavity, small intestinal mucosa basilar part is extensively congested, hemorrhage, and intestinal villi goblet cell is expanded, hypertrophy.As shown in Figure 4, treatment by Chinese herbs group piglet of the present invention has indivedual small intestinal mucosa epithelial cell generation degeneration, all the other no abnormality seens.
2.1.2.3 lamina propria and Submucosa pathological changes
As shown in Figure 5, antibiotic therapy group Small Intestine of Piglets wall lamina propria and Submucosa hyperemia, hemorrhage and edema, intestinal gland cells generation degeneration, downright bad, interstitial edema, lymphocytic infiltration, hypertrophy, eosinophilic granulocyte, neutrophil cell, plasma cell, macrophages infiltration, lymphatic nodule increases.And Fig. 6 is known, treatment by Chinese herbs group Small Intestine of Piglets wall lamina propria of the present invention and Submucosa show no obvious abnormalities.
2.2 Chinese medicine preparation of the present invention repair the efficacy test of Small Intestine of Piglets mucosa Ultracytochemical structure
2.2.1 test method
Test 3 Large-scale pig farm year November in August, 2014 to 2014 from surrounding area, Taiyuan; select the DLY three way cross piglet of weaning brash piglet 36 (28 ± 1) d, about body weight 10.04kg, be divided into 2 groups at random, often organize 3 repetitions; I group is antibiotic therapy group; II group of Chinese native medicine compound prescription pellet agent treatment group, carries out therapeutic test, respectively at after medication when 14 days; often group randomly draws 3; carotid artery sacrificed by exsanguination, abdominal cavity anatomic observation pathological change, aseptic collection small intestinal mucosa.The impact of Chinese medicine on diarrhoea ablactational baby pig small intestinal mucosa is observed by intestinal mucosa Electronic Speculum embedded section, concrete grammar is as follows: each piglet gets duodenum respectively, ileum 1-2cm organizes, after normal saline cleaning, being cut into 1mm size particles first puts into after 2% glutaraldehyde fixes 2hours, the buffer putting into 0.1mol/L Cacodylate as early as possible is again preserved, be embedded in epoxy resin through dehydration, the tissue of desired area makes ultrathin section (80um), and dye with saturated acetic acid uranyl and Reynolds (Reynold) Chinese holly edge lead plumbate, the arrangement of electricity Microscopic observation intestinal villi, goblet cell, lymphocyte cell film, Cytoplasm and nucleus, mitochondrion, endoplasmic reticulum, the pathological changes of the organelles such as lysosome.
2.2.2 result of the test
2.2.2.1 goblet cell electron microscopic section as shown in Figure 7, and the goblet cell nuclear matter of antibiotic therapy group leaks, endoplasmic reticulum vacuolation, mitochondrial swelling vacuolation, and ridge lacks, arrangement disorder.As shown in Figure 8, the cell membrane of the goblet cell for the treatment of by Chinese herbs group of the present invention is complete, and (developmental stage is different, so do not have nucleus), mitochondrion is complete, and ridge is complete, and nuclear membrane is complete.
2.2.2.2 intestinal microvillus electron microscopic section as shown in Figure 9, antibiotic therapy group enterocyte microvillus lodging shape, comes off obviously.As shown in Figure 10, the microvillus arrangement for the treatment of by Chinese herbs group small intestinal of the present invention is tight, neat smooth again, and in regular arrangement, adhesion complex complete as seen below microvillus, and actin, can see endoplasmic reticulum inside enterocyte.
2.2.2.3 intestinal wall lymphocyte electron microscopic section as shown in Figure 11, and the lymphocyte of antibiotic therapy group is the change of various pathologic, and mitochondrion enlargement, ridge lacks, and cell membrane, nuclear collapse, nuclear matter leaks.As shown in Figure 12, treatment by Chinese herbs group lymphocyte health of the present invention is complete, and nucleus, cell membrane are complete, mitochondrion, and interior knitmesh health is complete.
Result of the test shows, antibiotic therapy piglet diarrhea, heavy damage Small Intestine of Piglets mucous membrane tissue cytological structure, and compared with antibiotic therapy, Chinese medicine compound of the present invention can repair the Intestinal Mucosal Tissues structure of diarrhoea piglet and cyto-architectural damage, improve absorption function and the immunologic function of diarrhoea piglet intestinal mucosa, be the rehabilitation of piglet of suffering from diarrhoea, more efficiently method is provided.
beneficial effect of the present invention
(1) persistent period of Chinese medicine preparation of the present invention treatment piglet diarrhea be 2.15d than the 3.03d pole of matched group significantly shorten piglet diarrhea persistent period (
p<0.01), there is the anti-CaM antibody same compared with antibiotics.
(2) Chinese medicine preparation treatment group of the present invention no significant difference (P>0.05) compared with the content of antibiotic therapy group escherichia coli.And the quantity of lactobacillus and bacillus bifidus is extremely significantly greater than antibiotic therapy group (P<0.01) in Chinese medicine preparation treatment group intestinal contents of the present invention, and be significantly greater than blank group (P<0.05), result shows, Chinese medicine preparation of the present invention has the effect of roughly the same antibiotic suppression escherichia coli, and can the quantity of lactobacillus and bacillus bifidus in available protecting ablactational baby pig intestinal contents; The i.e. available protecting balance of beneficial bacteria of intestinal tract and Tiny ecosystem.
(3) the affect result of the test of the present invention on Small Intestine of Piglets feed nutrient digestibility shows, adds Chinese medicine preparation of the present invention and all can improve the apparent digestibility of piglet to organic substance, crude protein, crude fat, crude fibre, calcium and phosphorus compared with antibiotic in diet.Improve digestibility and the utilization rate of nutrient substance.
(4) Chinese medicine preparation treatment group piglet of the present invention body weight in time treating latter 2 weeks and 4 weekend increases very fast, growth performance is all significantly higher than antibiotic therapy group (P<0.05), and be significantly higher than blank group (P<0.05), show that Chinese medicine preparation of the present invention has the effect of the growth performance improving piglet.
(5) the present invention affects result of the test to Small Intestine of Piglets organizational structure and cellularity and shows, antibiotic therapy piglet diarrhea, heavy damage Small Intestine of Piglets mucous membrane tissue structure and cytological structure, and Chinese medicine preparation of the present invention can repair the Intestinal Mucosal Tissues structure of diarrhoea piglet and cyto-architectural damage, improve absorption function and the immunologic function of diarrhoea piglet intestinal mucosa, for the rehabilitation of piglet of suffering from diarrhoea provides more efficiently method.
Accompanying drawing explanation
Fig. 1 is lymph inflammatory cell infiltration tissue slice figure (HE400 ×) more in the chorioepithelium of antibiotic therapy Small Intestine of Piglets wall tissue.
Fig. 2 be treatment by Chinese herbs group Small Intestine of Piglets wall tissue of the present invention chorioepithelium in only have a little lymphocytic infiltration tissue slice figure (HE400 ×).
Fig. 3 has the epithelial cell and erythrocytic tissue's slice map (HE400 ×) that come off in the degeneration of antibiotic therapy group Small Intestine of Piglets mucosal epithelial cells, necrosis, enteric cavity.
Fig. 4 is that treatment by Chinese herbs group piglet of the present invention only has indivedual small intestinal mucosa epithelial cell generation degeneration, the tissue slice figure (HE400 ×) of all the other no abnormality seens.
Fig. 5 is that antibiotic therapy group Small Intestine of Piglets wall lamina propria and Submucosa are congested, hemorrhage and edema, intestinal gland cells generation degeneration, downright bad tissue slice figure (HE100 ×).
Fig. 6 is the tissue slice (HE400 ×) that treatment group Small Intestine of Piglets wall lamina propria of the present invention and Submucosa show no obvious abnormalities.
Fig. 7 is that the goblet cell nuclear matter of antibiotic therapy group leaks, endoplasmic reticulum vacuolation, mitochondrial swelling vacuolation, and ridge lacks, the electron microscopic section (JEM20000 ×) of arrangement disorder.
Fig. 8 is that the cell membrane of the goblet cell for the treatment of group of the present invention is complete, and (developmental stage is different, so do not have nucleus), mitochondrion is complete, and ridge is complete, the electron microscopic section (JEM12000 ×) that nuclear membrane is complete.
Fig. 9 is antibiotic therapy group enterocyte microvillus lodging shape, and come off obvious electron microscopic section (JEM20000).
Figure 10 is that the microvillus arrangement for the treatment of group small intestinal of the present invention is tight, neat smooth again, and in regular arrangement, adhesion complex complete as seen below microvillus and actin, can see endoplasmic reticulum (JEM12000 ×) inside enterocyte.
Figure 11 is the lymphocyte of antibiotic therapy group is the change of various pathologic, and mitochondrion enlargement, ridge lacks, cell membrane, nuclear collapse, the electron microscopic section (JEM30000 ×) that nuclear matter leaks.
Figure 12 is that treatment group lymphocyte health of the present invention is complete, and nucleus, cell membrane are complete, mitochondrion, the healthy complete electron microscopic section (JEM20000 ×) of interior knitmesh.
Detailed description of the invention
embodiment 1
Take Radix Codonopsis 1 kilogram, the Radix Astragali 1 kilogram, Rhizoma Atractylodis Macrocephalae (parched) 1.3 kilograms, Rhizoma dioscoreae 1.5 kilograms, Semen Myristicae 1 kilogram, Cortex Magnoliae Officinalis 1 kilogram, Pericarpium Citri Reticulatae 1 kilogram, Rhizoma Atractylodis 1 kilogram, Fructus Hordei Germinatus (parched to brown) 1 kilogram, Fructus Crataegi (parched to brown) 1 kilogram, Massa Medicata Fermentata (parched to brown) 1 kilogram, Radix Puerariae 1 kilogram, 1 kilogram, Radix Glycyrrhizae, after mixing, be crushed to 10-20 order; Above-mentioned medicinal powder is soaked 2h by the clear water getting whole quality of medicinal material 5 times, is then heated to boil, continues 30min, then solid-liquid separation with slow fire; In the solid phase of above-mentioned separation, add the clear water of whole quality of medicinal material 5 times again, be heated to boil, continue 20min with slow fire, then solid-liquid separation; Merge twice filtrate, be evaporated to the thick paste that proportion is 1.30-1.35, add the dextrin of thick paste quality 50%, make 60 object granules with granulator, pack.The using method of this Chinese medicine preparation is: take 10g every day by every piglet, by granule 5 times of 80 DEG C-90 DEG C warm water impulsive motions, stirs after fully dissolving and takes.
embodiment 2
Take Radix Codonopsis 1 kilogram, the Radix Astragali 1 kilogram, Rhizoma Atractylodis Macrocephalae (parched) 1.4 kilograms, Rhizoma dioscoreae 1.3 kilograms, Semen Myristicae 1 kilogram, Cortex Magnoliae Officinalis 1 kilogram, Pericarpium Citri Reticulatae 1 kilogram, Rhizoma Atractylodis 1 kilogram, Fructus Hordei Germinatus (parched to brown) 1 kilogram, Fructus Crataegi (parched to brown) 1 kilogram, Massa Medicata Fermentata (parched to brown) 1 kilogram, Radix Puerariae 1 kilogram, 1 kilogram, Radix Glycyrrhizae, after mixing, be crushed to 10-20 order; Above-mentioned medicinal powder is soaked 4h by the clear water getting whole quality of medicinal material 6 times, is then heated to boil, continues 20min, then solid-liquid separation with slow fire; In the solid phase of above-mentioned separation, add the clear water of whole quality of medicinal material 3 times again, be heated to boil, continue 30min with slow fire, then solid-liquid separation; Merge twice filtrate, be evaporated to the thick paste that proportion is 1.30-1.35, add the dextrin of thick paste quality 55%, make 60 object granules with granulator, pack.The using method of this Chinese medicine preparation is with embodiment 1.
embodiment 3
Take Radix Codonopsis 1 kilogram, the Radix Astragali 1 kilogram, Rhizoma Atractylodis Macrocephalae (parched) 1.35 kilograms, Rhizoma dioscoreae 1.4 kilograms, Semen Myristicae 1 kilogram, Cortex Magnoliae Officinalis 1 kilogram, Pericarpium Citri Reticulatae 1 kilogram, Rhizoma Atractylodis 1 kilogram, Fructus Hordei Germinatus (parched to brown) 1 kilogram, Fructus Crataegi (parched to brown) 1 kilogram, Massa Medicata Fermentata (parched to brown) 1 kilogram, Radix Puerariae 1 kilogram, 1 kilogram, Radix Glycyrrhizae, after mixing, be crushed to 10-20 order; Above-mentioned medicinal powder is soaked 4h by the clear water getting whole quality of medicinal material 5.5 times, is then heated to boil, continues 25min, then solid-liquid separation with slow fire; In the solid phase of above-mentioned separation, add the clear water of whole quality of medicinal material 4 times again, be heated to boil, continue 25min with slow fire, then solid-liquid separation; Merge twice filtrate, be evaporated to the thick paste that proportion is 1.30-1.35, add the dextrin of thick paste quality 53%, make 60 object granules with granulator, pack.The using method method of this Chinese medicine preparation is with embodiment 1.
Claims (3)
1. repair the Chinese medicine preparation of intestine of young pigs Tiny ecosystem and intestinal mucosa injury for one kind, it is characterized in that, this Chinese medicine composition is made up of the raw material of following mass parts, Radix Codonopsis 1, the Radix Astragali 1, Rhizoma Atractylodis Macrocephalae (parched) 1.3-1.4, Rhizoma dioscoreae 1.3-1.5, Semen Myristicae 1, Cortex Magnoliae Officinalis 1, Pericarpium Citri Reticulatae 1, Rhizoma Atractylodis 1, Fructus Hordei Germinatus (parched to brown) 1, Fructus Crataegi (parched to brown) 1, Massa Medicata Fermentata (parched to brown) 1, Radix Puerariae 1, Radix Glycyrrhizae 1.
2. the preparation method of the Chinese medicine preparation of reparation intestine of young pigs Tiny ecosystem according to claim 1 and intestinal mucosa injury comprises the steps:
(1) take Radix Codonopsis 1 part, the Radix Astragali 1 part, Rhizoma Atractylodis Macrocephalae (parched) 1.3-1.4 part, Rhizoma dioscoreae 1.3-1.5 part, Semen Myristicae 1 part, Cortex Magnoliae Officinalis 1 part, Pericarpium Citri Reticulatae 1 part, Rhizoma Atractylodis 1 part, Fructus Hordei Germinatus (parched to brown) 1 part, Fructus Crataegi (parched to brown) 1 part, Massa Medicata Fermentata (parched to brown) 1 part, Radix Puerariae 1 part, 1 part, Radix Glycyrrhizae, after mixing, be crushed to 10-20 order;
(2) get the medicinal powder immersion 2-4h that (1) prepares by whole quality of medicinal material 5-6 clear water doubly, be then heated to boil, continue 20-30min with slow fire, then solid-liquid separation;
(3) add whole quality of medicinal material 3-5 clear water doubly in the solid phase be separated in (2), be heated to boil, continue 20-30min with slow fire, then solid-liquid separation;
(4) merge (2), (3) twice filtrate, be evaporated to the thick paste that proportion is 1.30-1.35, add the dextrin of thick paste quality 50%-55%, make 60 object granules with granulator, pack.
3. the using method of the Chinese medicine preparation of reparation intestine of young pigs Tiny ecosystem according to claim 1 and intestinal mucosa injury is: take 10g every day by every piglet, by granule 5 times of 80 DEG C-90 DEG C warm water impulsive motions, stirs after fully dissolving and takes.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107050409A (en) * | 2017-03-10 | 2017-08-18 | 天津市畜牧兽医研究所 | A kind of herbal composite for adjusting intestine of young pigs health and preparation method and application |
| CN108888762A (en) * | 2018-08-15 | 2018-11-27 | 福建傲农生物科技集团股份有限公司 | A kind of composition of the metabolite containing probiotics |
| CN110583888A (en) * | 2019-10-10 | 2019-12-20 | 江西中医药大学 | Traditional Chinese medicine feed additive for preventing weaning emergency diarrhea of suckling pigs and preparation method thereof |
| CN113813345A (en) * | 2021-10-12 | 2021-12-21 | 吴晓林 | Fermented traditional Chinese medicine preparation for improving health vitality of young livestock and preparation method thereof |
| CN116870067A (en) * | 2023-08-10 | 2023-10-13 | 上海纳米技术及应用国家工程研究中心有限公司 | Traditional Chinese medicine composition for relieving VB12 malabsorption and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101579043A (en) * | 2009-04-13 | 2009-11-18 | 邹振可 | Chinese medicinal herb additive for promoting the growth of piglet and application thereof |
| CN102805271A (en) * | 2012-08-28 | 2012-12-05 | 安徽农业大学 | Anti-stress anti-diarrhea growth-promotion Chinese herbal medicine feed additive for weaned pig and preparation |
-
2015
- 2015-09-21 CN CN201510602803.9A patent/CN105125972B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101579043A (en) * | 2009-04-13 | 2009-11-18 | 邹振可 | Chinese medicinal herb additive for promoting the growth of piglet and application thereof |
| CN102805271A (en) * | 2012-08-28 | 2012-12-05 | 安徽农业大学 | Anti-stress anti-diarrhea growth-promotion Chinese herbal medicine feed additive for weaned pig and preparation |
Non-Patent Citations (1)
| Title |
|---|
| "断奶安", 《畜牧市场》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107050409A (en) * | 2017-03-10 | 2017-08-18 | 天津市畜牧兽医研究所 | A kind of herbal composite for adjusting intestine of young pigs health and preparation method and application |
| CN108888762A (en) * | 2018-08-15 | 2018-11-27 | 福建傲农生物科技集团股份有限公司 | A kind of composition of the metabolite containing probiotics |
| CN110583888A (en) * | 2019-10-10 | 2019-12-20 | 江西中医药大学 | Traditional Chinese medicine feed additive for preventing weaning emergency diarrhea of suckling pigs and preparation method thereof |
| CN113813345A (en) * | 2021-10-12 | 2021-12-21 | 吴晓林 | Fermented traditional Chinese medicine preparation for improving health vitality of young livestock and preparation method thereof |
| CN116870067A (en) * | 2023-08-10 | 2023-10-13 | 上海纳米技术及应用国家工程研究中心有限公司 | Traditional Chinese medicine composition for relieving VB12 malabsorption and preparation method thereof |
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