CN105123561A - Method for cultivating spongia - Google Patents

Abstract

The invention provides a method for cultivating spongia, and relates to the field of spongia. The method comprises transplanting sponge blocks as sponge matrixes, cutting the sponge matrixes into transplant small bodies, fixing the transplant small bodies on net ropes, cultivating in suspension to obtain spongia. The whole process is carried out in water. The exuberant regeneration capacity of spongia is utilized, a lot of spongia can propagate in natural sea areas through transplant, the biomass of spongia is large, the adhesive rate and survival rate of spongia can be greatly raised through fixing spongia transplants on net ropes, a batch of spongia can be cultivated in sea areas. The survival rate of sponge transplant small bodies can be raised to the maximum, the survival rate can reach up to 90 to 100 %. Knots of the net ropes are semi-closed, the other portions of the net ropes are opened, water flows smoothly during the growth of spongia. The net ropes facilitate fixation of spongia and later growth of the spongia. The method is easy to operate and suitable for mass production.

Description

Methods of Spongy Transplantation

technical field

The present invention relates to sponges, and more particularly to methods of transplanting sponges.

Background technique

Porifera, commonly known as sponge, is the simplest multicellular animal. It has no differentiation of tissues and organs. It has many symbiotic microorganisms and very diverse natural active products in its body. It is the most potential drug for development. source marine life. Since the content of natural active products in sponges is very low, drug development requires a large number of sponge organisms, and the artificial breeding technology of sponges was born.

The artificial cultivation of natural sponges for the purpose of obtaining active substances has not yet been carried out on a large scale at home and abroad. In China, only Zhang Wei et al. have conducted research on the purification of water bodies with luxuriant membrane sponges in the intertidal zone, and applied for several applications for the use of sponges in the purification of aquaculture water bodies. patent.

Contents of the invention

The object of the present invention is to provide a method for sponge animal transplantation which can obtain higher transplantation survival rate.

The present invention comprises the following steps:

1) Transplanting the sponge block as a sponge matrix;

In step 1), the sponge block can be selected with good growth, few surface fouling organisms, and uniform body color. The transplanting operation can be performed in the non-breeding season of the sponge, and the sponge is transplanted in the early morning when the water temperature is low. operate.

2) Cutting the spongy mother body into transplant bodies;

In step 2), the cutting of the sponge matrix into graft bodies can be cut into transplant bodies with a stainless steel knife, and the volume of each graft body can be 3-50 cm ³ , which is too large or too large. Small all easily lead to the death or loss of transplanted bodies.

3) After the transplanted body is fixed on the net rope and raised, the sponge can be harvested.

In step 3), the said fixing the transplanted body on the net rope can fix the transplanted body on the net rope according to the interval of 50-100 cm, and the said fixing on the net rope can adjust the binding rope so that the transplanted The small body is safely fixed on the net rope, and the net rope binds heavy objects and floating bodies at intervals of 1-2m, and the whole is suspended in a W shape in the sea area; the length of the net rope can be 10-100m; the net rope can be made of poly At least one of acrylic net rope, polyethylene net rope, polyamide net rope, polyester fiber net rope, etc.; the diameter of the single-strand rope constituting the net rope can be 1-8 mm, and the mesh diameter of the net rope can be 10-8 mm. 30mm; the width of the net rope can be 6-15 meshes/row; the laying distance of the tie rope can be 50-100cm; the hanging time can be 3-12 months.

The whole operation process of the present invention should be carried out in water, and the operation out of water is the main reason that causes air bubbles to enter the sponge ditch system, and is also the most important factor that causes the death of the sponge.

The present invention has the following outstanding advantages:

1. The present invention utilizes the vigorous regeneration ability of sponges, adopts the method of transplantation (explant) to carry out a large amount of propagation of sponges in natural sea areas, and obtains a large amount of sponge biomass. Combining the mesh rope method adopted by the present invention to fix the sponge graft body, the adhesion rate and survival rate of the sponge can be greatly improved, and the purpose of batch operation in sea areas can be realized.

2. Operating according to the technical points can maximize the survival rate of the sponge graft body, and the survival rate can reach 90-100%.

3. Except for the semi-closed knot, the rest of the rope is open, which can meet the needs of smooth water flow during the growth of the sponge.

4. The net rope is not only convenient for fixing the sponge, but also conducive to the growth of the sponge in the later stage.

5. Simple operation, suitable for mass production.

Detailed ways

The following examples will further illustrate the present invention.

The present invention comprises the following steps:

1) Carry out the transplantation of moss sponge in a certain sea area of Zhangpu, choose to collect the sponge mother body in the early morning, and use the sponge block as the sponge mother body for transplantation operation; the sponge block can be selected to have good growth, few surface fouling organisms, and uniform body color For the sponge block, the non-breeding season of the sponge can be selected for the transplanting operation, and the transplanting operation of the sponge is carried out in the morning when the water temperature is lower.

2) Cutting the sponge mother body into transplant bodies; said cutting the sponge mother body into transplant bodies can use a stainless steel knife to cut the sponge mother body into transplant bodies of uniform size, and the volume of each transplant body can be 3 to 50 cm ³ , too large or too small will easily lead to the death or loss of graft bodies.

3) After the transplanted body is fixed on the net rope and raised, the sponge can be harvested. Said fixing the transplanted body on the net rope can fix the transplanted body on the net rope according to the interval of 50-100 cm, and said fixing on the net rope can adjust the binding rope so that the transplanted body can be safely fixed on the net. On the rope, the net rope binds heavy objects and floating bodies at intervals of 1-2m, and the whole is suspended in a W shape in the sea area; the length of the net rope can be 10-100m; the net rope can be made of polypropylene net rope, polyethylene net rope, etc. At least one of net rope, polyamide net rope, polyester fiber net rope, etc.; the diameter of the single-strand rope constituting the net rope can be 1-8mm, and the mesh diameter of the net rope can be 10-30mm; the width of the net rope It can be 6-15 meshes/row; the laying distance of the tie-in ropes can be 50-100 cm; the hanging time can be 3-12 months.

Experiments have shown that when the sponges are transplanted at a distance of 1 m, the survival rate of 120 sponge transplanted bodies is 100%, and the growth rate of the sponges within 2 months can reach 470%.

At present, there are no special tools or equipment for sponge transplantation at home and abroad. Stones, wood boards, PVC boards, ropes and other materials are usually used as the substrate for the fixed growth of sponges, and then cotton ropes or cable ties are used to bind the sponge transplants. It is loaded down with trivial details, and what is more important is to use the sponge of rope binding under the washing of running water, and its attachment rate is low, and survival rate is also not high. These methods are currently only suitable for small-scale sample processing in laboratories and cannot be applied to production in sea areas.

Claims (10)

1. the method for spongia transplanting, is characterized in that comprising the following steps:

1) sponge block is carried out graft procedure as sponge parent;

2) sponge parent is cut into transplanting corpusculum;

3) transplanting corpusculum is fixed on nettle after hanging culture, can spongia be gathered in the crops.

2. as claimed in claim 1 spongia transplant method, it is characterized in that in step 1) in, described sponge block select grow fine, surperficial fouling organism is few, the uniform sponge block of body colour.

3. the spongia method of transplanting as claimed in claim 1, is characterized in that in step 1) in, described in carry out graft procedure be select the anestrous season of sponge, the graft procedure carrying out sponge early morning that water temperature is lower.

4. the method for spongia transplanting as claimed in claim 1, it is characterized in that in step 2) in, the described transplanting corpusculum that is cut into by sponge parent uses stainless steel knife that sponge parent is cut into transplanting corpusculum of uniform size, and the volume of each transplanting corpusculum is 3 ~ 50cm ³.

5. the method for spongia transplanting as claimed in claim 1, is characterized in that in step 3) in, described being fixed on nettle by transplanting corpusculum is fixed on nettle by transplanting corpusculum according to the spacing of 50 ~ 100cm; Describedly being fixed on nettle, is regulate tying rope to make transplanting corpusculum be fixed on nettle, and nettle, by the spacing binding weight of 1 ~ 2m and buoyancy aid, overallly hangs on sea area in W shape.

6. the method for spongia transplanting as claimed in claim 1, is characterized in that in step 3) in, the length of described nettle is 10 ~ 100m.

7. the method for spongia transplanting as claimed in claim 1, is characterized in that in step 3) in, described nettle adopts at least one in polypropylene nettle, polyethylene nettle, polyamide nettle, polyester fiber nettle.

8. the method for spongia transplanting as claimed in claim 1, is characterized in that in step 3) in, the diameter forming the sub-thread rope of nettle is 1 ~ 8mm, and the mesh diameter of nettle is 10 ~ 30mm; The width of nettle is 6 ~ 15 meshes/OK.

9. the method for spongia transplanting as claimed in claim 1, is characterized in that in step 3) in, the spacing that lays of described tying rope is 50 ~ 100cm.

10. the method for spongia transplanting as claimed in claim 1, is characterized in that in step 3) in, the time of described hanging culture is 3 ~ 12 months.