CN105123561B - The method of spongia transplanting - Google Patents

The method of spongia transplanting Download PDF

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Publication number
CN105123561B
CN105123561B CN201510413995.9A CN201510413995A CN105123561B CN 105123561 B CN105123561 B CN 105123561B CN 201510413995 A CN201510413995 A CN 201510413995A CN 105123561 B CN105123561 B CN 105123561B
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China
Prior art keywords
nettle
sponge
transplanting
corpusculum
spongia
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CN105123561A (en
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王德祥
欧徽龙
陈军
丁少雄
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Xiamen University
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Xiamen University
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Cultivation Of Seaweed (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Hydroponics (AREA)

Abstract

The method of spongia transplanting, is related to spongia.Graft procedure is carried out using sponge block as sponge parent;Sponge parent is cut into transplanting corpusculum;Transplanting corpusculum is fixed on nettle after hanging culture, spongia can be harvested.Whole operation process should carry out in water.Utilize the power of regeneration that sponge is vigorous, the largely expansion for carrying out natural waters sponge using the method for transplanting is numerous, obtains a large amount of spongy biological amounts, and the fixation of sponge implant body is carried out using nettle method, the adhesive rate and survival rate of sponge can be greatly improved, realizes the purpose of sea area batch operation.The survival rate of sponge implant corpusculum can be improved to greatest extent, and survival rate is up to 90~100%.Nettle in addition to the place of knotting is semi-enclosed, rest part be it is open, can satisfy the demand in sponge growth course to unobstructed water flow.Nettle had not only facilitated the fixation of sponge, but also was conducive to the growth in sponge later period.It is easy to operate, it is suitable for mass production.

Description

The method of spongia transplanting
Technical field
The present invention relates to spongias, more particularly, to the method for spongia transplanting.
Background technique
Porifera (Porifera) is commonly called as spongia (sponge), is simplest multicellular animals, its not no group The differentiation with organ is knitted, possesses numerous symbiotic microorganisms and very various natural active product in vivo, is most to develop The medicine source marine organisms of potentiality.Since content of the natural active product in sponge is very low, medicament research and development needs a large amount of sponge The artificial culture technology of biology, sponge arises.
Natural sponge artificial breeding not yet extensive development at home and abroad for the purpose of obtaining active material, it is domestic rarely seen It the research for the purifying water body carried out to intertidal zone marine sponge Hymeniacidon perleve such as defends, and has applied several sponge being applied to purifying aquaculture The patent of water body.
Summary of the invention
The purpose of the present invention is to provide a kind of methods for spongia transplanting that can get higher transplanting success.
The present invention the following steps are included:
1) graft procedure is carried out using sponge block as sponge parent;
In step 1), the sponge block can select grow fine, surface fouling organism is few, the uniform sponge block of body colour, The anestrous season of sponge may be selected in the graft procedure that carries out, and water temperature lower early morning carries out the graft procedure of sponge.
2) sponge parent is cut into transplanting corpusculum;
In step 2), it is described by sponge parent be cut into transplanting corpusculum stainless steel knife can be used that sponge parent is cut into size The volume of uniform transplanting corpusculum, each transplanting corpusculum can be 3~50cm3, too big or too small be all easy to cause transplanting corpusculum Dead or loss.
3) transplanting corpusculum is fixed on nettle after hanging culture, spongia can be harvested.
In step 3), described transplanting corpusculum is fixed on nettle can will transplant corpusculum according to the spacing of 50~100cm It is fixed on nettle, it is described to be fixed on nettle, tying rope is adjusted, is fixed on transplanting corpusculum on nettle safely, nettle presses 1 The spacing binding weight and floating body of~2m, it is whole to hang on sea area in W shape;The length of the nettle can be 10~100m;It is described At least one of polypropylene nettle, polyethylene nettle, polyamide nettle, polyester fiber nettle etc. can be used in nettle;Constitute net The diameter of the sub-thread rope of rope can be 1~8mm, and the mesh diameter of nettle can be 10~30mm;The width of nettle can be 6~15 Mesh/row;The spacing that lays of the tying rope can be 50~100cm;The time of the hanging culture can be 3~12 months.
Whole operation process of the invention should carry out in water, cause bubble to enter sponge ditch system from water operation Main cause, and cause sponge death in most important factor.
The invention has the following outstanding advantages:
1, the present invention power of regeneration vigorous using sponge carries out natural waters sea using the method for transplanting (explant) Continuous largely expansion is numerous, obtains a large amount of spongy biological amounts.The nettle method used in conjunction with the present invention carries out the fixation of sponge implant body, can To greatly improve the adhesive rate and survival rate of sponge, the purpose of sea area batch operation is realized.
2, the survival rate of sponge implant corpusculum can be improved to greatest extent by carrying out operation according to technical essential, and survival rate is reachable 90-100%.
3, nettle is in addition to the place of knotting is semi-enclosed, rest part be it is open, can satisfy in sponge growth course Demand to unobstructed water flow.
4, nettle had not only facilitated the fixation of sponge, but also was conducive to the growth in sponge later period.
5, easy to operate, it is suitable for mass production.
Specific embodiment
The present invention is further illustrated for following embodiment.
The present invention the following steps are included:
1) transplanting that tongue fur sponge is carried out in Zhangpu sea area, selects early morning to carry out the collection of sponge parent, sponge block is made Graft procedure is carried out for sponge parent;The sponge block can select grow fine, surface fouling organism is few, the uniform sponge of body colour The anestrous season of sponge may be selected in block, the graft procedure that carries out, and water temperature lower early morning carries out the graft procedure of sponge.
2) sponge parent is cut into transplanting corpusculum;It is described by sponge parent be cut into transplanting corpusculum can be used stainless steel knife will be extra large Continuous parent is cut into transplanting corpusculum of uniform size, and the volume of each transplanting corpusculum can be 3~50cm3, too big or too small all easy Lead to the death or loss of transplanting corpusculum.
3) transplanting corpusculum is fixed on nettle after hanging culture, spongia can be harvested.It is described to be fixed on transplanting corpusculum Transplanting corpusculum can be fixed on nettle according to the spacing of 50~100cm on nettle, it is described to be fixed on nettle, tying is adjusted Rope is fixed on transplanting corpusculum on nettle safely, and nettle bundlees weight and floating body by the spacing of 1~2m, whole to hang in W shape Sea area;The length of the nettle can be 10~100m;Polypropylene nettle, polyethylene nettle, polyamide web can be used in the nettle At least one of rope, polyester fiber nettle etc.;The diameter for constituting the sub-thread rope of nettle can be 1~8mm, and the mesh of nettle is straight Diameter can be 10~30mm;The width of nettle can be 6~15 mesh/rows;The tying rope lay spacing can for 50~ 100cm;The time of the hanging culture can be 3~12 months.
It is demonstrated experimentally that the transplanting of sponge is carried out using the spacing of 1m, and the survival rate 100% of 120 sponge implant corpusculums, 2 A month sponge growth rate is up to 470%.
Currently, there is no the tool or equipment specifically for sponge implant both at home and abroad, stone is generallyd use, plank, PVC board, Then the substrate that the materials such as rope are grown as sponge fixation bundlees sponge implant body with cotton cord or band, operation It is relatively cumbersome, it is often more important that for the sponge bundled using rope under the washing away of flowing water, adhesive rate is low, and survival rate is not yet It is high.These methods are only applicable to the small scale samples processing in laboratory at present, can not apply to sea area production.

Claims (2)

1. the method for spongia transplanting, it is characterised in that the following steps are included:
1) graft procedure is carried out using sponge block as sponge parent;The sponge block select grow fine, surface fouling organism is few, The uniform sponge block of body colour;The graft procedure that carries out is the anestrous season for selecting sponge, and water temperature lower early morning carries out sea Continuous graft procedure;
2) sponge parent is cut into transplanting corpusculum;
It is described by sponge parent be cut into transplanting corpusculum be that sponge parent is cut into transplanting corpusculum of uniform size using stainless steel knife, The volume of each transplanting corpusculum is 3~50cm3
3) transplanting corpusculum is fixed on nettle after hanging culture, spongia can be harvested;
Described transplanting corpusculum is fixed on nettle is to be fixed on transplanting corpusculum on nettle according to the spacing of 50~100cm;Institute State and be fixed on nettle, be adjust tying rope make transplant corpusculum be fixed on nettle, nettle by 1~2m spacing binding weight and Floating body, it is whole to hang on sea area in W shape;The length of the nettle is 10~100m;The diameter for constituting the sub-thread rope of nettle is 1 ~8mm, the mesh diameter of nettle are 10~30mm;The width of nettle is 6~15 mesh/rows;Between the laying of the tying rope Away from for 50~100cm;The time of the hanging culture is 3~12 months.
2. the method for spongia transplanting as described in claim 1, it is characterised in that in step 3), the nettle uses poly- third At least one of alkene nettle, polyethylene nettle, polyamide nettle, polyester fiber nettle.
CN201510413995.9A 2015-07-15 2015-07-15 The method of spongia transplanting Active CN105123561B (en)

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CN105123561B true CN105123561B (en) 2018-12-04

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Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109717109A (en) * 2019-02-01 2019-05-07 厦门大学 A kind of fixed point transplantation method of lichee sponge
CN111670846B (en) * 2020-04-20 2021-10-15 厦门大学 In-situ cultivation method for sponge larvae

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101099452A (en) * 2006-07-05 2008-01-09 中国科学院大连化学物理研究所 Laboratory artificial cultivating method for intertidal zone marine sponge

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101099452A (en) * 2006-07-05 2008-01-09 中国科学院大连化学物理研究所 Laboratory artificial cultivating method for intertidal zone marine sponge

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
密克罗尼西亚的海绵养殖;林国成 译;《国外水产》;19930630(第2期);18-20 *
海参苗—海绵—大型海藻循环养殖技术;付晚涛 等;《水产科学》;20131015;第32卷(第10期);559-566 *
繁茂膜海绵的实验室养殖;张卫 等;《中国水产科学》;20050830;第12卷(第4期);430-436 *

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