CN106489710B - A kind of high algin strain development of sargassum kjellmanianum Yendo and cultural method - Google Patents
A kind of high algin strain development of sargassum kjellmanianum Yendo and cultural method Download PDFInfo
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- CN106489710B CN106489710B CN201610958376.2A CN201610958376A CN106489710B CN 106489710 B CN106489710 B CN 106489710B CN 201610958376 A CN201610958376 A CN 201610958376A CN 106489710 B CN106489710 B CN 106489710B
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- 241000195474 Sargassum Species 0.000 title claims abstract description 75
- 235000010443 alginic acid Nutrition 0.000 title claims abstract description 44
- 229920000615 alginic acid Polymers 0.000 title claims abstract description 44
- 238000000034 method Methods 0.000 title claims abstract description 41
- 238000011161 development Methods 0.000 title claims abstract description 10
- 235000013601 eggs Nutrition 0.000 claims abstract description 18
- 238000012216 screening Methods 0.000 claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 230000016087 ovulation Effects 0.000 claims abstract description 11
- 235000013311 vegetables Nutrition 0.000 claims abstract description 10
- 230000035800 maturation Effects 0.000 claims abstract description 6
- 230000003252 repetitive effect Effects 0.000 claims abstract description 3
- 241000196324 Embryophyta Species 0.000 claims description 49
- 239000013535 sea water Substances 0.000 claims description 17
- 238000005286 illumination Methods 0.000 claims description 13
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims description 7
- 235000017491 Bambusa tulda Nutrition 0.000 claims description 7
- 241001330002 Bambuseae Species 0.000 claims description 7
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims description 7
- 239000011425 bamboo Substances 0.000 claims description 7
- 239000004744 fabric Substances 0.000 claims description 7
- 238000012360 testing method Methods 0.000 claims description 6
- 241000195493 Cryptophyta Species 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 238000004804 winding Methods 0.000 claims description 5
- 235000007199 Panicum miliaceum Nutrition 0.000 claims description 4
- 229920002978 Vinylon Polymers 0.000 claims description 3
- 244000022185 broomcorn panic Species 0.000 claims description 3
- 239000003653 coastal water Substances 0.000 claims description 3
- 230000002706 hydrostatic effect Effects 0.000 claims description 3
- 230000000384 rearing effect Effects 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- 102000002322 Egg Proteins Human genes 0.000 claims description 2
- 108010000912 Egg Proteins Proteins 0.000 claims description 2
- 210000004681 ovum Anatomy 0.000 claims description 2
- 239000004033 plastic Substances 0.000 claims description 2
- 238000010586 diagram Methods 0.000 description 2
- 241000512259 Ascophyllum nodosum Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241001474374 Blennius Species 0.000 description 1
- 241000251511 Holothuroidea Species 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 241000195475 Sargassaceae Species 0.000 description 1
- 241001262105 Sargassum muticum Species 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 244000022203 blackseeded proso millet Species 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G33/00—Cultivation of seaweed or algae
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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- Life Sciences & Earth Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Environmental Sciences (AREA)
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Abstract
The invention discloses a kind of high algin strain development of sargassum kjellmanianum Yendo and cultural methods, and the sargassum kjellmanianum Yendo frond with receptacle grown naturally is temporarily supported, is dried in the shade, fertilized eggs are diffused, indoor raising of seedling moves back to sea to cultivate to sargassum kjellmanianum Yendo and grow up to seedling length to 2mm；When not formed receptacle is cultivated at sea, sargassum kjellmanianum Yendo continuation of its algin content higher than 31% is measured with mass method and is at sea cultivated to receptacle maturation, wherein healthy and strong complete, the uniform frond of length plants vegetables for selection；It plants vegetables and is placed in aquarium after culture has ovulation to receptacle, repetitive operation " sargassum kjellmanianum Yendo nursery ", " screening " and " mix and adopt " three year of process；In 9~12 DEG C of water temperature of third year, algin content is detected with mass method, reaching 31% with 95% single plant frond algin content, the above are standards, if algin content is still not up to target, continue to screen using above-mentioned steps.The method of the present invention can improve sargassum kjellmanianum Yendo algin content, improve unit area sargassum kjellmanianum Yendo algin yield.
Description
Technical field
The invention belongs to seaweed oxygen―increasing reagent fields, and in particular to a kind of high algin strain development of sargassum kjellmanianum Yendo and cultivation
Method.
Background technique
Sargassum kjellmanianum Yendo (Sargassum muticum) is Sargassaceae, the perennial kelp of Sargassum, is distributed widely in
The north of China Huanghai Sea and Sea And Bohai Sea Coast.Frond length 1m or more is grown in the seabed of stormy waves lesser inner bay and neritic area naturally
On rock reef.The average content of algin is 28% in sargassum kjellmanianum Yendo frond, is one of the primary raw material for manufacturing algin.In recent years,
With the fast development of China's economical alga application study, sargassum is constantly soaring in the demand of glue and holothurian feed.Cause
This, natural resources is not able to satisfy actual demand by subtracting, although the means for having sargassum kjellmanianum Yendo artificial breeding to supplement as resource, it is supported
Grow limitation of the area by sea area area.This just needs to study a kind of method that can be improved sargassum kjellmanianum Yendo algin content, improves
The algin yield of unit area sargassum kjellmanianum Yendo.This patent utilizes the bio-diversity of sargassum kjellmanianum Yendo, carries out high algin content sea broomcorn millet
The cultivation of sub- strain.
Summary of the invention
The technical problem to be solved by the present invention is to be directed to the deficiencies in the prior art, and it is high to provide a kind of sargassum kjellmanianum Yendo
Algin strain development and cultural method, the method that can be improved sargassum kjellmanianum Yendo algin content improve unit area sargassum kjellmanianum Yendo
Algin yield.
In order to achieve the above-mentioned object of the invention, the present invention adopts the following technical scheme:
A kind of high algin strain development of sargassum kjellmanianum Yendo and cultural method, comprising the following steps:
(1) sargassum kjellmanianum Yendo nursery:
Naturally the sargassum kjellmanianum Yendo frond with receptacle grown is put into aquarium and is temporarily supported, is temporarily supported to receptacle
On have to pull out after a large amount of ovulations and dry in the shade, the frond to dry in the shade, which is put into seawater, diffuses fertilized eggs, and fertilized eggs are splashed to being placed with seedling
In the nursery pond of curtain, fertilized eggs are attached on seedling curtain to carry out nursery；To seedling it is long to 2mm move back to sea cultivated to
Sargassum kjellmanianum Yendo grows up to；
(2) it screens:
Sargassum kjellmanianum Yendo single plant is carried out when receptacle has not yet been formed in the sargassum kjellmanianum Yendo in step (1) and sea surface temperature is at 10-11 DEG C
Screening: sargassum kjellmanianum Yendo is cut off from middle part, top lease making mass method measures sargassum kjellmanianum Yendo of its algin content higher than 31% and closes screening
Continue at sea to cultivate to receptacle maturation, selection it is required that will meet and be partially sandwiched on seedling rope under the sargassum kjellmanianum Yendo that screening requires
Wherein healthy and strong complete, the uniform frond of length plants vegetables；
(3) it mixes and adopts:
Planting vegetables of obtaining of step (2), which is placed in aquarium on culture to receptacle, ovulation phenomenon, by this sargassum kjellmanianum Yendo
Continue to adopt step according to mixed described in " screening " described in " sargassum kjellmanianum Yendo nursery ", step (2) described in step (1) and step (3)
Cultivating to receptacle has ovulation phenomenon, such three year of process flow repetitive operation；
(4) ore grade indexes:
In 9~12 DEG C of water temperature of third year, the top half of the sargassum kjellmanianum Yendo frond of culture is taken to be detected with mass method brown
Phycocolloid content, reaching 31% with 95% single plant frond algin content, the above are standards, if algin content is still not up to
Target then continues to screen using above-mentioned steps.
In above-mentioned technical proposal, sargassum kjellmanianum Yendo nursery described in step (1), concrete operations the following steps are included:
1. temporarily supporting: at 14-16 DEG C of water temperature, the sargassum kjellmanianum Yendo stalwartness frond with receptacle so grown is derived from, with going out
Bacterium seawater scrubs the sundries on frond surface, is subsequently placed in aquarium and is temporarily supported, and temporarily support 20 days or so has greatly to receptacle
Amount ovulation；
2. nursery: thering is the sargassum kjellmanianum Yendo frond largely ovulated to pull out on receptacle 1. step is temporarily supported after, be placed on clean modeling
It dries in the shade on material cloth；Then frond bolting silk package is placed in the sterilizing seawater that temperature is 16 DEG C and diffuses fertilized eggs, by frond in sea
Slightly pendulum is pulled out after washing in water；Fertilized eggs on bolting silk are eluted in watering can, fertilized eggs are uniformly splashed and place seedling to prior
It is long to 2mm to seedling that nursery is carried out in the nursery pond of curtain (3)；
3. forming: bind bamboo pole (2) between two hawsers (1), for fixing the spacing of two hawsers, prevent hawser because
Stormy waves winding；By step 2. in the quadrangle of the seedling curtain (3) with 2mm long seedling lie on PVC frame and stretch flat seedling curtain, PVC frame
Frame quadrangle is put in sea after being lain on hawser respectively with tether and is formed；Form to seedling length be 40cm or so when, by 40
The seedling of ± 3cm long is taken out from seedling curtain, is sandwiched on seedling rope (4) and carries out folder Miao Yangcheng, the both ends of seedling rope are lain in PVC frame
On frame and stretch flat seedling rope, PVC frame quadrangle, which continues to be put in sea after being lain in respectively on hawser with tether, is formed；When plant is long
When to 60cm or so, the plant of 60 ± 5cm long is re-started into folder Miao Yangcheng, continues culture until sargassum kjellmanianum Yendo grows up to.
Preferably, the step 1. in, temporarily support condition are as follows: 16 DEG C of water temperature, 25-35 μm of olm of illumination-2s-1, periodicity of illumination
12L:12D changes 1/3 seawater daily and slightly scrubs frond.
Preferably, the step 2. in, the described time of drying in the shade is 1-2h；The bolting silk is the bolting silk of 25 mesh；Institute
The pendulum stated washes the time as 30min；The seedling curtain is the nylon door curtain made of cloth, is vinylon cloth rope；Described uniformly splashes fertilized eggs
Onto seedling curtain, density of splashing is 10-15/cm2。
Preferably, the step 2. in, rearing condition are as follows: build sunshade net above nursery pond, keep most intense light irradiation
6000lx is inflated in nursery pond hereinafter, preceding 72h hydrostatic culture；Hereafter, carry out miniflow Aquaponic, when seedling it is long to 2mm when,
Move to coastal waters culture.
Preferably, the step 3. in, the bamboo pole between hawser, each other between be divided into 2m；The children of 40 ± 3cm long
When carrying out folder seedling of seedling, folder seedling density are 10-12 plants/m, strain spacing 8-10cm, and the spacing of seedling rope is 10-15cm；60 ± 5cm long
Plant re-start folder seedling when, strain spacing be 12-15cm, restrict spacing 20-25cm.
Preferably, the step 3. in, form condition are as follows: in the early stage, seedling curtain is suitably hung deeply when within seedling 10cm,
Intensity of illumination control periodically removes the sundries such as miscellaneous algae and the mud scum on seedling curtain in 8000-10000lx；In mid-term, seedling length
When 20-40cm, water layer where seedling curtain is promoted, intensity of illumination control removes in 15000lx or so and grows too fast and excessively slow children
Seedling keeps growth of seedling density in 2 plants/cm2；Seedling it is long to 40cm or so when carry out folder Miao Yangcheng；Seedling is long to 60cm or so
Shi Chongxin presss from both sides seedling, and seedling rope is promoted to the shoaling layer of 20-30cm, and control light intensity is in 20000-25000lx, and periodically pendulum washes plant
To remove the sundries such as mud scum, and the frond of winding is separated, continues culture to sargassum kjellmanianum Yendo and grow up to.
In above-mentioned technical proposal, the concrete operations of the screening of single plant described in step (2) are as follows: healthy and strong with frond, complete,
The uniform sargassum kjellmanianum Yendo of length is cut off as test object, by test object from middle part, and upper part is cleaned, surveyed after drying through mass method
Fixed sargassum kjellmanianum Yendo of its algin content higher than 31% meets screening and requires；1000 plants are met the lower part for the sargassum kjellmanianum Yendo that screening requires
Divide and be individually sandwiched in Miao Shengshang again, seedling rope both ends are lain on PVC frame and are stretched seedling rope, strain spacing 12-15cm, rope spacing
15-20cm, continuation are at sea cultivated to receptacle maturation；It is complete that 30-60 plants of stalwartnesses are chosen in this 1000 plants of mature sargassum kjellmanianum Yendos
Whole, the uniform frond of length plants vegetables.
In above-mentioned technical proposal, the condition cultivated in aquarium described in step (3) are as follows: 16 DEG C of water temperature, illumination 25-35
μmolm-2s-1, periodicity of illumination 12L:12D changes 1/3 seawater daily, and slightly scrubs frond, on temporarily feeding 10 days or so to receptacle
There is ovulation phenomenon.
In above-mentioned technical proposal, the concrete operations of ore grade indexes described in step (4) are as follows: take 1 rope extra large at interval of 10 ropes
Broomcorn millet takes this upper evenly spaced 10 plants of frond of restricting, takes 10 ropes altogether, totally 100 plants of fronds；The brown of single plant frond is detected with mass method
Phycocolloid content, reaching 31% with 95% single plant frond algin content, the above are standards；If algin content is still not up to
Target then continues to screen using above-mentioned steps.
The present invention is compared with prior art: the present invention utilizes the bio-diversity of sargassum kjellmanianum Yendo, carries out high algin content sea
The cultivation of broomcorn millet strain, the method that can be improved sargassum kjellmanianum Yendo algin content improve the algin yield of unit area sargassum kjellmanianum Yendo.
Detailed description of the invention
In Fig. 1, the method for the present invention, seedling curtain lies in the structural schematic diagram on PVC frame；
In Fig. 2, the method for the present invention, seedling rope lies in the structural schematic diagram on PVC frame；
Wherein: 1, hawser；2, bamboo pole；3, seedling curtain；4, Miao Sheng.
Specific embodiment
Method of the invention is specifically described combined with specific embodiments below:
Embodiment 1: the high algin strain development of the valgar island sargassum kjellmanianum Yendo in Rongcheng
A kind of high algin strain development of sargassum kjellmanianum Yendo and cultural method, comprising the following steps:
(1) sargassum kjellmanianum Yendo nursery:
1. temporarily supporting: at 16 DEG C of seawater surface temperature, the sargassum kjellmanianum Yendo stalwartness frond for taking the valgar island in Rongcheng to grow naturally, with sterilizing
Seawater scrubs the sundries on frond surface, is placed in aquarium and is temporarily supported.Temporary feeding condition: 16 DEG C of water temperature, 25-35 μm of olm of illumination- 2s-1, periodicity of illumination 12L:12D changes 1/3 seawater daily, and slightly scrubs frond, and it is temporarily 6 days feeding, it is found that a large amount of receptacles start to hang
Ovum；
2. nursery: frond being pulled out and is placed on clean plastic cloth the 2h that dries in the shade, then frond is wrapped up with 25 mesh thin,tough silk and putting
Fertilized eggs are diffused in the sterilizing seawater that temperature is 16 DEG C, slightly pendulum washes 30min in the seawater by frond, then pulls out.It will sieve
Fertilized eggs on thin,tough silk are eluted in watering can, and fertilized eggs are uniformly splashed into nursery pond, and the average value of 10 microscopies is 12, thing
The vinylon door curtain made of cloth first is placed in nursery pond, specification is length × wide=120cm × 40cm；
Rearing condition: building sunshade net above nursery pond, keeps most intense light irradiation 6000lx hereinafter, preceding 72h hydrostatic culture,
It is inflated in nursery pond.Hereafter, carry out miniflow Aquaponic, when seedling it is long to 2mm when, move to valgar island coastal waters culture, it is sea water advanced
5m；
3. forming: binding bamboo pole between two hawsers, bamboo pole interval 2m is prevented for fixing the spacing of two hawsers
Hawser is wound because of stormy waves.Seedling curtain is fixed on interior minister × wide=120cm × 40cm PVC frame, and stretches flat seedling curtain, frame
Quadrangle is lain on hawser respectively.Seedling curtain is suitably hung deeply when in the early stage, within seedling 10cm, intensity of illumination is controlled in 9000-
10000lx, the every 3 days miscellaneous algaes removed on seedling curtain；In mid-term, seedling length 10-40cm, water layer where seedling curtain, illumination are promoted
Strength control removes in 15000lx or so and grows too fast and excessively slow seedling, keeps growth of seedling density in 2 plants/cm2；To
Seedling it is long to 40cm or more when, choose the shoot of 40 ± 3cm, be sandwiched in Miao Shengshang, folder seedling density is 10 plants/m, strain spacing 10cm,
Seedling rope both ends are lain on PVC frame, are stretched, seedling rope spacing 15cm.In plant length to 60cm or so, the life of 60 ± 5cm is selected
Long uniform plant, presss from both sides on Miao Yu seedling rope again, and strain spacing is 15cm, and restrict spacing 20cm, and seedling rope is promoted to the shallow of 30cm
Water layer, pendulum washes plant to remove the sundries such as mud scum within every 3 days, and the frond of winding is separated, and continues culture to sargassum kjellmanianum Yendo and grows up to (such as
Shown in Fig. 1 and Fig. 2).
(2) it screens:
Grow up to the phase in sargassum kjellmanianum Yendo, when not yet arriving receptacle formation (11 DEG C of sea surface temperature).Select frond healthy and strong, length
Uniform frond is cut off from middle part by sargassum kjellmanianum Yendo, takes top to clean, drying, measures algin content, remainder with mass method
Continue to be clipped on seedling rope and cultivate.Screening background quantity is 1000 plants, and 42 plants of fronds that algin content reaches 32% are individually weighed
It is newly sandwiched in Miao Shengshang, strain spacing 15cm, restrict spacing 15cm, and seedling rope both ends lie on PVC frame and are stretched seedling rope, continue to cultivate
To receptacle maturation, select again wherein 33 plants of stalwartnesses it is complete, the uniform frond of length plants vegetables.
(3) it mixes and adopts:
33 plants of mature planting vegetables are divided into the algae section of 20cm, is placed in aquarium and is temporarily supported.Temporarily support condition: water temperature 16
DEG C, 25-35 μm of olm of illumination-2s-1, periodicity of illumination 12L:12D changes 1/3 seawater daily, and inflation suspends culture, and slightly scrubs algae
Body, temporarily supporting 5 days or so has ovulation phenomenon to receptacle.And repeated for three years according to above-mentioned nursery, cultivation and screening process.
(4) ore grade indexes:
At third year, 11 DEG C of water temperature, the top half of the marine sargassum kjellmanianum Yendo frond cultivated is taken to be detected with mass method brown
Phycocolloid content.Sampling method: taking 1 rope sargassum kjellmanianum Yendo at interval of 10 ropes, takes this upper evenly spaced 10 plants of frond of restricting, takes 10 ropes altogether,
Totally 100 plants of fronds, single plant detect algin content.The frond algin content that testing result shows 85% reaches 32%, because
This increases by 1 year screening operation, until the 4th year, the frond algin content that testing result shows 95% reach 32%.
Examples detailed above is technical conception and technical characteristics to illustrate the invention, can not be limited with this of the invention
Protection scope.The equivalent transformation or modification that all essence according to the present invention is done, should all cover in protection scope of the present invention
Within.
Claims (10)
1. a kind of high algin strain development of sargassum kjellmanianum Yendo and cultural method, which comprises the following steps:
(1) sargassum kjellmanianum Yendo nursery:
Naturally the sargassum kjellmanianum Yendo frond with receptacle grown is put into aquarium and is temporarily supported, is temporarily supported to having on receptacle
It pulls out and dries in the shade after a large amount of ovulations, the frond to dry in the shade, which is put into seawater, diffuses fertilized eggs, and fertilized eggs are splashed to being placed with seedling curtain
In nursery pond, fertilized eggs are attached on seedling curtain to carry out nursery；It is cultivated to long move back to 2mm to sea of seedling to extra large broomcorn millet
Son grows up to；
(2) it screens:
The screening of sargassum kjellmanianum Yendo single plant is carried out when receptacle has not yet been formed in the sargassum kjellmanianum Yendo in step (1) and sea surface temperature is at 10-11 DEG C:
Sargassum kjellmanianum Yendo is cut off from middle part, top lease making mass method measures sargassum kjellmanianum Yendo of its algin content higher than 31% and meets screening requirement,
It will meet to be partially sandwiched on seedling rope under the sargassum kjellmanianum Yendo that screening requires and continue at sea to cultivate to receptacle maturation, selection is wherein good for
Strong complete, the uniform frond of length plants vegetables；
(3) it mixes and adopts:
Planting vegetables of obtaining of step (2), which is placed in aquarium on culture to receptacle, ovulation phenomenon；
(4) sargassum kjellmanianum Yendo for obtaining step (3) continues according to described in " sargassum kjellmanianum Yendo nursery ", step (2) described in step (1)
Described in " screening " and step (3) it is mixed adopt step and cultivate to receptacle have ovulation phenomenon, such process flow repetitive operation three
Year；
(5) ore grade indexes:
In 9-12 DEG C of water temperature of third year, takes the top half mass method of the sargassum kjellmanianum Yendo frond of culture to detect algin and contain
Amount, reaching 31% with 95% single plant frond algin content, the above are standards, if algin content is still not up to target,
Then continue to screen using above-mentioned steps.
2. the method according to claim 1, wherein sargassum kjellmanianum Yendo nursery as described in step (1), concrete operations packet
Include following steps:
1. temporarily supporting: at 14-16 DEG C of water temperature, the sargassum kjellmanianum Yendo stalwartness frond with receptacle so grown is derived from, with sterilizing sea
The sundries for washing brush frond surface, is subsequently placed in aquarium and is temporarily supported, and temporarily supporting 20 days or so has a large amount of rows to receptacle
Ovum；
2. nursery: thering is the sargassum kjellmanianum Yendo frond largely ovulated to pull out on receptacle 1. step is temporarily supported after, be placed on clean plastic cloth
On dry in the shade；Then frond bolting silk package is placed in the sterilizing seawater that temperature is 16 DEG C and diffuses fertilized eggs, in the seawater by frond
Slight pendulum is pulled out after washing；Fertilized eggs on bolting silk are eluted in watering can, fertilized eggs are uniformly splashed and place seedling curtain (3) to prior
Nursery pond in carry out nursery long to 2mm to seedling；
3. forming: bind bamboo pole (2) between two hawsers (1) prevents hawser because of stormy waves for fixing the spacing of two hawsers
Winding；By step 2. in the seedling curtain (3) with 2mm long seedling be fixed on PVC frame and stretch flat seedling curtain, PVC frame quadrangle is used
Tether is put in sea after being lain on hawser respectively and is formed；Form to seedling length be 40cm or so when, by 40 ± 3cm's long
Seedling is taken out from seedling curtain, is sandwiched on seedling rope (4) and carries out folder Miao Yangcheng, the both ends of seedling rope are fixed on PVC frame simultaneously
Stretch flat seedling rope, PVC frame quadrangle, which continues to be put in sea after being lain in respectively on hawser with tether, is formed；When plant is long to 60cm
When left and right, the plant of 60 ± 5cm long is re-started into folder Miao Yangcheng, continues culture until sargassum kjellmanianum Yendo grows up to.
3. according to the method described in claim 2, it is characterized in that, the step 1. in, temporarily support condition are as follows: 16 DEG C of water temperature,
25-35 μm of olm of illumination-2s-1, periodicity of illumination 12L:12D changes 1/3 seawater daily and slightly scrubs frond.
4. according to the method described in claim 2, it is characterized in that, the step 2. in, the described time of drying in the shade is 1-2h；
The bolting silk is the bolting silk of 25 mesh；The pendulum washes the time as 30min；The seedling curtain is the vinylon door curtain made of cloth；The general
Fertilized eggs are uniformly splashed onto seedling curtain, and density of splashing is 10-15/cm2。
5. according to the method described in claim 2, it is characterized in that, the step 2. in, rearing condition are as follows: on nursery pond
Sunshade net is built by side, keeps most intense light irradiation 6000lx hereinafter, preceding 72h hydrostatic culture, is inflated in nursery pond；Hereafter, it carries out micro-
Flowing water culture, when seedling it is long to 2mm when, move to coastal waters culture.
6. according to the method described in claim 2, it is characterized in that, the step 3. in, the bamboo pole between hawser, each other it
Between between be divided into 2m；When carrying out folder seedling of the seedling of 40 ± 3cm long, folder seedling density are 10-12 plants/m, strain spacing 8-10cm, seedling
The spacing of rope is 10-15cm；When the plant of 60 ± 5cm long re-starts folder seedling, strain spacing is 12-15cm, and restrict spacing 20-
25cm。
7. according to the method described in claim 2, it is characterized in that, the step 3. in, form condition are as follows: in the early stage, children
Seedling curtain is suitably hung deeply when within seedling 10cm, intensity of illumination control in 8000-10000lx, periodically remove miscellaneous algae on seedling curtain and
Mud scum sundries；In mid-term, seedling length 20-40cm, water layer where seedling curtain is promoted, intensity of illumination is controlled in 15000lx or so,
And remove and grow too fast and excessively slow seedling, keep growth of seedling density in 2 plants/cm2；Seedling it is long to 40cm or so when pressed from both sides
Seedling is formed；Seedling it is long to 60cm or so when press from both sides seedling again, and seedling rope is promoted to the shoaling layer of 20-30cm, control light intensity exists
20000-25000lx, periodically pendulum washes plant to remove mud scum sundries, and the frond of winding is separated, and continues culture to sargassum kjellmanianum Yendo
Grow up to.
8. the method according to claim 1, wherein the concrete operations of the screening of single plant described in step (2) are such as
Under:, complete, length uniform sargassum kjellmanianum Yendo healthy and strong using frond is cut off as test object, by test object from middle part, and upper part is washed
Only, sargassum kjellmanianum Yendo of its algin content higher than 31%, which is measured, through mass method after drying meets screening requirement；Meet screening for 1000 plants
It is required that the lower part of sargassum kjellmanianum Yendo be individually sandwiched in Miao Shengshang again, seedling rope both ends are lain on PVC frame and are stretched seedling rope, strain
Spacing 12-15cm, rope spacing 15-20cm, continuation are at sea cultivated to receptacle maturation；In this 1000 plants of mature sargassum kjellmanianum Yendos
30-60 plants of healthy and strong complete, the uniform fronds of length are chosen to plant vegetables.
9. the method according to claim 1, wherein the condition cultivated in aquarium described in step (3) are as follows:
16 DEG C of water temperature, 25-35 μm of olm of illumination-2s-1, periodicity of illumination 12L:12D changes 1/3 seawater daily, and slightly scrubs frond, support to
There is ovulation phenomenon on receptacle.
10. the method according to claim 1, wherein the concrete operations of ore grade indexes described in step (5)
Are as follows: 1 rope sargassum kjellmanianum Yendo is taken at interval of 10 ropes, this upper evenly spaced 10 plants of frond of restricting is taken, takes 10 ropes altogether, totally 100 plants of fronds；Use matter
Amount method detects the algin content of single plant frond, and reaching 31% with 95% single plant frond algin content, the above are standards；If
Algin content is still not up to target, then continues to screen using above-mentioned steps.
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