CN103444506A - Artificial breeding method for fry of Sargassum muticum - Google Patents

Artificial breeding method for fry of Sargassum muticum Download PDF

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CN103444506A
CN103444506A CN201310429009XA CN201310429009A CN103444506A CN 103444506 A CN103444506 A CN 103444506A CN 201310429009X A CN201310429009X A CN 201310429009XA CN 201310429009 A CN201310429009 A CN 201310429009A CN 103444506 A CN103444506 A CN 103444506A
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seedling
curtain
pond
vegetables
collecting
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CN103444506B (en
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刘玮
刘梦侠
詹冬梅
吴海一
王翔宇
徐智广
丁刚
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SHANDONG PROVINCE MARINE CULTURE INSTITUTE
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SHANDONG PROVINCE MARINE CULTURE INSTITUTE
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management

Abstract

The invention provides an artificial breeding method for fry of Sargassum muticum. According to the technical scheme, the method includes: 1, seedling selection and maturity promotion, namely selecting healthy Sargassum muticum seedlings having a receptacle, gripping the seedlings, and drying the seedlings in the shade to stimulate fertilized eggs to diffuse when eggs are rested on the receptacles of Sargassum muticum; 2, indoor seedling collection and cultivation, namely placing a seedling curtain and a glass slide at the bed of a seedling collection basin to collect seedlings and microscopically examine density of attached seedlings, and cultivating the seedlings in sea when the seedlings grow to the proper size; 3, sea cultivation and breeding, namely, selecting a sea curtain-hanging manner according to water temperature, controlling light, and selecting a proper culture water layer to allow seedlings of Sargassum muticum to grow healthily. The method has the advantages that the seedlings of Sargassum muticum are bred artificially according to the sexual propagation features of Sargassum muticum by means of techniques such as seedling maturity promotion, indoor seedling collection and cultivation and sea breeding.

Description

A kind of method that sargassum kjellmanianum Yendo fingerlings artificial is bred
Technical field
The invention belongs to the mariculture technology field, specifically, relate to and a kind ofly utilize sargassum kjellmanianum Yendo sexual reproduction to carry out the method for artificial breeding with the sargassum kjellmanianum Yendo seed that secures good health.
Background technology
Sargassum kjellmanianum Yendo is a kind of algae with economic worth, can be used as the raw material of feed and extraction algin.In addition, sargassum kjellmanianum Yendo also has the biological nature of Adsorption of Heavy Metals, therefore can be used as the instrument marine alga that purifies greater coasting area.Sargassum kjellmanianum Yendo is grown seedlings study limitation indoor at present, does not form a set of complete technical method.Therefore, the technical scheme that industrially also there is no the method for sargassum kjellmanianum Yendo artificial breeding.
Summary of the invention
A kind of method that provides sargassum kjellmanianum Yendo fingerlings artificial to breed is provided the object of the invention, utilizes sargassum kjellmanianum Yendo sexual propagation characteristics, by technical methods such as the accelerating that plants vegetables, indoor seeding cultivation, maricultures, thereby realizes that the sargassum kjellmanianum Yendo fingerlings artificial breeds.
The present invention mainly solves following technical problem:
1, the accelerating that plants vegetables: sargassum kjellmanianum Yendo generally is grown in below the water surface, and a large amount of synchronous ripe sargassum kjellmanianum Yendos of more difficult disposable acquisition plant vegetables, and concentrate, to obtain a large amount of sargassum kjellmanianum Yendo fertilized eggs are the prerequisites of carrying out artificial breeding.
2, indoor seeding is cultivated: control the time that suitable collect seedling density and seedling are plunged into the commercial sea.
3, the marine cultivation and cultivation: sargassum kjellmanianum Yendo is prone to de-seedling at sea and causes emergence rate low while cultivating, how cultivating and cultivate out healthy healthy and strong sargassum kjellmanianum Yendo seedling at sea is a problem demanding prompt solution.
In order to realize technical problem to be solved by this invention, technical scheme of the present invention is, a kind of method that sargassum kjellmanianum Yendo fingerlings artificial is bred, and described method following steps are carried out:
(1), plant vegetables and select and accelerating: start to collect sargassum kjellmanianum Yendo when the sea area temperature during at 11-13 ℃ and plant vegetables, plant vegetables and select receptacle to physically well develop, the marine alga of frond stalwartness, by the sargassum kjellmanianum Yendo filtered out plant vegetables clean with seawater flushing after, pressed from both sides seedling with the seedling rope, the 5-7 strain plants vegetables folder during seedling that to gather be 1 bunch, each bunch of spacing setting, after the folder seedling, the seedling rope is bundled in the indoor raising of seedling pond accelerating cultivation that planted vegetables on scaffold, illumination is controlled at 5000-30000 lx, water temperature is controlled at 12-15 ℃, salinity is not less than 25 ‰, every day, flowing water was cultivated 3-4 hour, nursery pond is cultivated about 10-30 days, can obtain the sargassum kjellmanianum Yendo maturation of hanging ovum plants vegetables,
(2), collect seedling: choose the sargassum kjellmanianum Yendo maturation taking-up nursery pond that plants vegetables, In Shade dry in the shade stimulation 25-35 minute, then be placed in the pond of collecting seedling that the depth of water is not less than 500mm, collect seedling at the bottom of pond and complete in advance the seedling curtain for collecting seedling, put into 3-5 slide, for microscopy spatfall density simultaneously, within every 4-8 hour, stir in the pond of collecting seedling and plant vegetables once, after planting vegetables and diffusing 24-48 hour, start microscopic examination glass carrier, on slide, the sargassum kjellmanianum Yendo triploidy rate is not less than 80/cm 2the time, will plant vegetables and take out the pond of collecting seedling, throw aside, should in pond, supplement and plant vegetables if microscopy density is on the low side, until reach above-mentioned density, in the process of collecting seedling, illumination is controlled at 3000-10000lx, and water temperature is not higher than 19 ℃, and salinity is not less than 25 ‰;
(3), support temporarily in the pond of collecting seedling: the hydrostatic in the pond of collecting seedling of the seedling curtain after collecting seedling continues to cultivate 2-3 days, the sargassum kjellmanianum Yendo fertilized egg firm attachment of diffusing wait planting vegetables on the seedling curtain after, the pond of collecting seedling needs to change water and continue to cultivate, collecting seedling, to change the water frequency be 3-4 day/time in pond, seedling curtain artificial left and right shake every day 2-3 time, prevent that the assorted algae of dirt from adhering to, and within every 3 days, microscopy is observed sargassum kjellmanianum Yendo seedling development situation once, when the seedling average length is greater than 1mm, the seedling curtain can be transferred marine the continuation and cultivate;
(4), mariculture: when the seawater water temperature below 19 ℃, during the not enough 20000lx of top layer illumination under water, the seedling curtain can take level to keep hanging the mode of growing, be about to seedling curtain two ends respectively tether be fixed on the parallel floating well rope in two, mariculture district, seedling curtain level is immersed under sea, on the seedling curtain, have seedling to adhere to one facing up, if illumination is excessively strong, suitably reduction level keeps hanging water layer, within every 2-3 days, the pendulum curtain rinses once, when seawater water temperature to 19-20 ℃, change into and hang breeding way from above, being about to the seedling curtain takes off from two floating well ropes, the one side that after the doubling of seedling curtain, will have seedling to adhere to is outside, one end of seedling curtain is fixed on floating well rope, other end tether binding pendant thing falls under sea level the seedling curtain, the degree of depth is hung in adjustment from above, illumination is not higher than 20000lx, hanging the every 3-5 of seedling curtain days pendulum curtains from above rinses once, when water temperature rises to more than 22 ℃, the sargassum kjellmanianum Yendo poor growth, should add profundal zone to reduce illumination to 10000-15000 lx, and should suitably increase and wash away frequency, put only harmful organisms invasion and attack seedling curtain, if water temperature is reduced to below 19 ℃, can change the flat continuation cultivation of hanging into by hanging from above,
After mariculture 3-4 month, can obtain the healthy sargassum kjellmanianum Yendo seedling of 40-60mm, the seedling curtain can be fixed on artificial algal reef to be thrown in coastal waters, for restoration of the ecosystem, or the sargassum kjellmanianum Yendo seedling is continued at sea to cultivate to adult.
Condition of culture when support temporarily in the pond of collecting seedling is: illumination is not higher than 30000lx, and water temperature is not higher than 20 ℃, and salinity is not less than 25 ‰, solubility content of inorganic nitrogen in (DIN) 15-20 μ mol/L, Soluble Inorganic Phosphorus content (DIP) 0.3-0.5 μ mol/L.
In process is supported temporarily in the pond of collecting seedling, if the seedling curtain is covered and needs to adopt current to rinse by dirt, flow rate of water flow should, not higher than 0.8m/s, cause seedling with protection against erosion.
During the daily management of mariculture, when on the seedling curtain, mud scum or invertebrate adhere in a large number, need to adopt sea to wash away mode to avoid growth of seedling to be affected, the Wash flow flow velocity is 1 m/s, while adhering to a large amount of assorted algae on the seedling curtain, need the assorted algae of excision in time in case assorted algae is covered the seedling curtain and de-seedling occurs.
In plant vegetables selection and accelerating, the nylon rope that seedling rope used is diameter 8-15mm.
In plant vegetables selection and accelerating, each bunch of spacing 80-120mm.
The main points of the technical scheme that as seen through the above technical solutions, technical solution problem of the present invention adopts are as follows:
1, plant vegetables and select and accelerating: the healthy sargassum kjellmanianum Yendo of selecting to have receptacle plants vegetables after the folder seedling, supports temporarily controlled light at nursery pond, by changing the water management water temperature upper limit.When the ovum of extension is arranged on the sargassum kjellmanianum Yendo receptacle, dry in the shade stimulation fertilized egg is diffused.
2, indoor seeding is cultivated: at the bottom of the pond of collecting seedling, the placement chemical fibre door curtain made of cloth and slide are respectively used to collect seedling and microscopy spatfall density.The fertilized egg of diffusing that plants vegetables is attached on the door curtain made of cloth, by spatfall quantity on microscopic examination glass carrier, controls the density of collecting seedling.After having collected seedling, first hydrostatic is cultivated flowing water again and is cultivated, the cultivation of can plunging into the commercial sea when the extremely suitable size of growth of seedling.
3, the marine cultivation and cultivation: just select seedling curtain offshore suspended mode according to water temperature, controlled light is selected suitable cultivation water layer, and, in conjunction with marine management method, prevents that mud scum, harmful organisms from affecting the healthy growth of sargassum kjellmanianum Yendo seedling.
Because sargassum kjellmanianum Yendo has stronger biological adsorption ability to heavy metal in seawater (nickel, cadmium etc.), so can be used as the instrument marine alga of purifying sea water, the cultivating large area sargassum kjellmanianum Yendo coordinate artificial algal reef to build submarine forest.The present invention, by propagating the sargassum kjellmanianum Yendo seedling that can obtain 4-6cm artificially, can be artificial algae field the sargassum kjellmanianum Yendo seed is provided, for the Offshore Ecology environment remediation.
Sargassum kjellmanianum Yendo is as aquaculture feed, and available sea cucumber, abalone culture, can alleviate the dependence of the aquatic products economic animal cultivation such as sea cucumber, abalone to extraordinary marine alga (sargassum thunbergii, Zostera marina etc.) to a certain extent.
Algal polysaccharide in sargassum kjellmanianum Yendo, polyphenol have the important biomolecule activity and can be used for biomedicine field.
Sargassum kjellmanianum Yendo artificial raise seedling method of the present invention provides the sargassum kjellmanianum Yendo seedling of cultivation use; solved current sargassum kjellmanianum Yendo natural resources under-supply and to the destruction of natural resources; by propagating artificially, sargassum kjellmanianum Yendo can be sea cucumber, abalone high-quality bait is processed provides important raw and processed materials; promote the development of sea cucumber, abalone culture industry; and can be used as the instrument marine alga that purifies the coastal ocean environment, significant for the protection nearshore marine environment.
The present invention has realized the sargassum kjellmanianum Yendo artificial breeding.The major advantage performance is as follows:
1, realize that fertilized egg obtains in batches: sargassum kjellmanianum Yendo is owing to be difficult for obtaining a large amount of ripe fertilized eggs, thereby artificial breeding research is confined to indoorly always, do not realize scale.
2, grasp the suitable density of collecting seedling: the sargassum kjellmanianum Yendo density of collecting seedling is low, and during the later stage mariculture, assorted algae adheres to seriously, and management difficulty is large; Density is too high, and growth of seedling speed is influenced, and seedling is little is subject to the animal invasion and attack.The suitable density of collecting seedling, be conducive to the seedling Fast Growth, reduces the final-period management cost.
3, the marine nurturing period of seedling: nursery pond is cultivated overlong time, can directly increase aquaculture cost; Time is too short, the seedling storm resisting capacity a little less than, easy de-seedling.Hold the suitable mariculture time, be conducive to reduce aquaculture cost, improve emergence rate.
4, mariculture mode: regulate breeding way according to marine actual conditions, employing level cultivation when water temperature is low, than the intense light irradiation stimulating growth; When water temperature is high, adopt vertically cultivation, reduce light intensity and avoid assorted algae to adhere in a large number.The marine tour managed at ordinary times in addition, prevents that mud scum and invertebrate from adhering to.
In sum, sargassum kjellmanianum Yendo artificial breeding method of the present invention, can realize sargassum kjellmanianum Yendo seedling artificial breeding, and the sargassum kjellmanianum Yendo seed secures good health.
Embodiment
The method of a kind of sargassum kjellmanianum Yendo fingerlings artificial of the present invention being bred below in conjunction with embodiment is elaborated, and described method step is as follows:
1, plant vegetables and select and accelerating: the sea area temperature starts to collect sargassum kjellmanianum Yendo and plants vegetables in the time of 12 ℃, plants vegetables and selects receptacle to physically well develop, the marine alga of frond stalwartness.By the sargassum kjellmanianum Yendo filtered out plant vegetables clean with seawater flushing after, pressed from both sides seedling with the nylon rope of diameter 8-15 mm, while pressing from both sides seedling, the 5-7 strain plants vegetables, and to gather be 1 bunch, every bunch of spacing 10cm.After the folder seedling, the seedling rope is bundled in nursery pond the accelerating cultivation that planted vegetables on scaffold, and illumination is controlled at 5000-30000 lx, and water temperature is controlled at 12-15 ℃, and salinity is not less than 25 ‰, and every day, flowing water was cultivated 3-4 hour.Nursery pond is cultivated about 10-30 days, can obtain the maturation of hanging ovum and plant vegetables.
2, collect seedling: choose the maturation taking-up nursery pond that plants vegetables, In Shade dry in the shade stimulation 30 minutes, then be placed in the pond of collecting seedling that the depth of water is not less than 50cm.Collect seedling at the bottom of pond and complete in advance the chemical fibre door curtain made of cloth for collecting seedling, put into 3-5 slide, for microscopy spatfall density simultaneously.Within every 4-8 hour, stir in the pond of collecting seedling and plant vegetables once, after planting vegetables and diffusing 24-48h, start microscopic examination glass carrier, when triploidy rate is not less than 80/cm2 on slide, will plants vegetables and take out the pond of collecting seedling, throw aside.Can in pond, supplement and plant vegetables if microscopy density is on the low side, until reach proper density.In the process of collecting seedling, illumination is controlled at 3000-10000lx, and water temperature is not higher than 19 ℃, and salinity is not less than 25 ‰.
3, support temporarily in the pond of collecting seedling: the hydrostatic in the pond of collecting seedling of the seedling curtain after collecting seedling continues to cultivate 2-3 days, the fertilized egg firm attachment of diffusing wait planting vegetables on the seedling curtain after, the pond of collecting seedling needs to change water and continues to cultivate.Collecting seedling, to change the water frequency be 3-4 day/time in pond, and seedling curtain artificial left and right shake every day 2-3 time prevents that the dirt algae of mixing from adhering to.Condition of culture is: illumination is not higher than 30000lx, and water temperature is not higher than 20 ℃, and salinity is not less than 25 ‰, solubility content of inorganic nitrogen in (DIN, DIN is the solubility inorganic nitrogen) 15-20 μ mol/L, Soluble Inorganic Phosphorus content (DIP, DIP Soluble Inorganic Phosphorus) 0.3-0.5 μ mol/L.Within every 3 days, microscopy is observed sargassum kjellmanianum Yendo seedling development situation once, and when the seedling average length is greater than 1mm, the seedling curtain can be plunged into the commercial sea and be continued to cultivate.In process is supported in the pond of collecting seedling temporarily, if the seedling curtain is covered and needs to adopt current to rinse by dirt, flow rate of water flow should be higher than 0.8m/s, and takes the circumstances into consideration to reduce flow velocity according to actual conditions, with protection against erosion, causes and falls in a large number seedling.
4, mariculture: the seawater water temperature is below 19 ℃, during the not enough 20000lx of top layer illumination under water, the seedling curtain can be taked the flat mode of growing that keeps hanging, be about to seedling curtain two ends respectively tether be fixed on the parallel floating well rope in two, mariculture district, seedling curtain level is immersed under sea, be 90 ° of angles with floating well rope level, on the seedling curtain, have seedling to adhere to one facing up.If illumination is excessively strong, can suitably reduce the flat water layer of hanging, within every 2-3 days, the pendulum curtain rinses once.When seawater water temperature to 19-20 ℃, change into and hang breeding way from above, be about to the seedling curtain and take off from two floating well ropes, the one side that after the doubling of seedling curtain, will have seedling to adhere to is outside, and an end of seedling curtain is fixed on floating well rope, and other end tether binding dropstone falls under sea level the seedling curtain.The degree of depth is hung in adjustment from above, and illumination, not higher than 20000lx, is hung the every 3-5 of seedling curtain days pendulum curtains from above and rinsed once.When water temperature rises to more than 22 ℃, sargassum kjellmanianum Yendo growth is very slow, can add profundal zone to reduce illumination to 10000-15000 lx, and should suitably increase and wash away frequency, puts only harmful organisms invasion and attack seedling curtain.If water temperature is reduced to below 19 ℃, can change the flat continuation cultivation of hanging into by hanging from above.During marine daily management, when mud scum or invertebrate adhere in a large number on the seedling curtain, need to adopt sea to wash away mode to avoid growth of seedling to be affected, the Wash flow flow velocity is 1 m/s; While adhering to a large amount of assorted algae on the seedling curtain, need the assorted algae of excision in time in case assorted algae is covered the seedling curtain and de-seedling occurs.
After mariculture 3-4 month, can obtain the healthy sargassum kjellmanianum Yendo seedling of 4-6cm.The seedling curtain can be fixed on artificial algal reef to be thrown in coastal waters, for restoration of the ecosystem.Also the sargassum kjellmanianum Yendo seedling can be continued at sea to cultivate to adult, using as sea cucumber, abalone culture feed.

Claims (6)

1. the method that the sargassum kjellmanianum Yendo fingerlings artificial is bred, is characterized in that, described method following steps are carried out:
(1), plant vegetables and select and accelerating: start to collect sargassum kjellmanianum Yendo when the sea area temperature during at 11-13 ℃ and plant vegetables, plant vegetables and select receptacle to physically well develop, the marine alga of frond stalwartness, by the sargassum kjellmanianum Yendo filtered out plant vegetables clean with seawater flushing after, pressed from both sides seedling with the seedling rope, the 5-7 strain plants vegetables folder during seedling that to gather be 1 bunch, each bunch of spacing setting, after the folder seedling, the seedling rope is bundled in the indoor raising of seedling pond accelerating cultivation that planted vegetables on scaffold, illumination is controlled at 5000-30000 lx, water temperature is controlled at 12-15 ℃, salinity is not less than 25 ‰, every day, flowing water was cultivated 3-4 hour, nursery pond is cultivated about 10-30 days, can obtain the sargassum kjellmanianum Yendo maturation of hanging ovum plants vegetables,
(2), collect seedling: choose the sargassum kjellmanianum Yendo maturation taking-up nursery pond that plants vegetables, In Shade dry in the shade stimulation 25-35 minute, then be placed in the pond of collecting seedling that the depth of water is not less than 500mm, collect seedling at the bottom of pond and complete in advance the seedling curtain for collecting seedling, put into 3-5 slide, for microscopy spatfall density simultaneously, within every 4-8 hour, stir in the pond of collecting seedling and plant vegetables once, after planting vegetables and diffusing 24-48 hour, start microscopic examination glass carrier, on slide, the sargassum kjellmanianum Yendo triploidy rate is not less than 80/cm 2the time, will plant vegetables and take out the pond of collecting seedling, throw aside, should in pond, supplement and plant vegetables if microscopy density is on the low side, until reach above-mentioned density, in the process of collecting seedling, illumination is controlled at 3000-10000lx, and water temperature is not higher than 19 ℃, and salinity is not less than 25 ‰;
(3), support temporarily in the pond of collecting seedling: the hydrostatic in the pond of collecting seedling of the seedling curtain after collecting seedling continues to cultivate 2-3 days, the sargassum kjellmanianum Yendo fertilized egg firm attachment of diffusing wait planting vegetables on the seedling curtain after, the pond of collecting seedling needs to change water and continue to cultivate, collecting seedling, to change the water frequency be 3-4 day/time in pond, seedling curtain artificial left and right shake every day 2-3 time, prevent that the assorted algae of dirt from adhering to, and within every 3 days, microscopy is observed sargassum kjellmanianum Yendo seedling development situation once, when the seedling average length is greater than 1mm, the seedling curtain can be transferred marine the continuation and cultivate;
(4), mariculture: when the seawater water temperature below 19 ℃, during the not enough 20000lx of top layer illumination under water, the seedling curtain can take level to keep hanging the mode of growing, be about to seedling curtain two ends respectively tether be fixed on the parallel floating well rope in two, mariculture district, seedling curtain level is immersed under sea, on the seedling curtain, have seedling to adhere to one facing up, if illumination is excessively strong, suitably reduction level keeps hanging water layer, within every 2-3 days, the pendulum curtain rinses once, when seawater water temperature to 19-20 ℃, change into and hang breeding way from above, being about to the seedling curtain takes off from two floating well ropes, the one side that after the doubling of seedling curtain, will have seedling to adhere to is outside, one end of seedling curtain is fixed on floating well rope, other end tether binding pendant thing falls under sea level the seedling curtain, the degree of depth is hung in adjustment from above, illumination is not higher than 20000lx, hanging the every 3-5 of seedling curtain days pendulum curtains from above rinses once, when water temperature rises to more than 22 ℃, the sargassum kjellmanianum Yendo poor growth, should add profundal zone to reduce illumination to 10000-15000 lx, and should suitably increase and wash away frequency, put only harmful organisms invasion and attack seedling curtain, if water temperature is reduced to below 19 ℃, can change the flat continuation cultivation of hanging into by hanging from above,
After mariculture 3-4 month, can obtain the healthy sargassum kjellmanianum Yendo seedling of 40-60mm, the seedling curtain can be fixed on artificial algal reef to be thrown in coastal waters, for restoration of the ecosystem, or the sargassum kjellmanianum Yendo seedling is continued at sea to cultivate to adult.
2. method according to claim 1, it is characterized in that: the condition of culture while supporting temporarily in the pond of collecting seedling is: illumination is not higher than 30000lx, and water temperature is not higher than 20 ℃, and salinity is not less than 25 ‰, solubility content of inorganic nitrogen in 15-20 μ mol/L, Soluble Inorganic Phosphorus content 0.3-0.5 μ mol/L.
3. method according to claim 2 is characterized in that: in process is supported temporarily in the pond of collecting seedling, if the seedling curtain is covered and needs to adopt current to rinse by dirt, flow rate of water flow should, not higher than 0.8m/s, cause seedling with protection against erosion.
4. method according to claim 2, it is characterized in that: during the daily management of mariculture, when on the seedling curtain, mud scum or invertebrate adhere in a large number, need to adopt sea to wash away mode to avoid growth of seedling to be affected, the Wash flow flow velocity is 1 m/s, while adhering to a large amount of assorted algae on the seedling curtain, need the assorted algae of excision in time in case assorted algae is covered the seedling curtain and de-seedling occurs.
5. method according to claim 1 is characterized in that: plant vegetables select with accelerating in, the nylon rope that seedling rope used is diameter 8-15mm.
6. method according to claim 1 is characterized in that: plant vegetables select with accelerating in, each bunch of spacing 80-120mm.
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CN106489710A (en) * 2016-11-03 2017-03-15 山东省海洋生物研究院 A kind of high algin strain development of sargassum kjellmanianum Yendo and cultural method
CN107047280A (en) * 2017-06-23 2017-08-18 山东省海洋生物研究院 A kind of intensive method of collecting seedling of sargassum kjellmanianum Yendo
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