CN105123275A - Culture method for edible fungus culture medium - Google Patents
Culture method for edible fungus culture medium Download PDFInfo
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- CN105123275A CN105123275A CN201510632170.6A CN201510632170A CN105123275A CN 105123275 A CN105123275 A CN 105123275A CN 201510632170 A CN201510632170 A CN 201510632170A CN 105123275 A CN105123275 A CN 105123275A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
- C05D3/02—Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
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- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention discloses a culture method for an edible fungus culture medium, belongs to the technical field of preparation of edible fungus culture materials and aims to solve the technical problems in the prior art that pesticide residues exist in fruit bodies of edible fungi cultured by cut-log and the culture method and edible fungi cultured by the culture method cannot be used for leisure enjoyment. The culture method comprises the following steps: S1, putting cut-log into a culture box, adding a mixture of corn straw powder and quicklime in the box to bury the cut-log, and carrying out primary high-temperature sterilization on the mixture in the culture box at the temperature of 100-150 DEG C for 15-25 min; S2, after primary sterilization, when the culture box is cooled to room temperature, inoculating food-grade fungi strains in the culture box; S3, carrying out mycelium culture on the culture box after inoculation, and after mycelia are distributed over the whole culture box, carrying out secondary sterilization at the temperature of 60-80 DEG C for 30-45 min; S4, placing a separating net on a culture medium of the culture box and covering the culture box with a plastic film. The fruit bodies of edible fungi cultured by the culture method disclosed by the invention are free of pesticide residues.
Description
Technical field
The invention belongs to and prepare culture medium of edible fungus technical field, be specifically related to a kind of cultural method of culture medium of edible fungus.
Background technology
In prior art with section carpentery workshop for culture medium of edible fungus cultivate fruit body of edible fungi time, be after Duan Mu covers with mycelia, Duan Mu is buried in the earth or frame be placed on cultivate place to cultivate fruit body of edible fungi.Duan Mu is buried in the earth or frame be placed on cultivate place cultivate fruit body of edible fungi time be vulnerable to miscellaneous bacteria, earth pollution and there is more insect pest.For avoiding the pollution of miscellaneous bacteria and preventing insect pest, in cultivation fruit body of edible fungi process, inevitably use agricultural chemicals, make the fruit body of edible fungi cultivated leave residue of pesticide.And the incubation of edible mushroom, edible fungus can not be introduced leisure and view and admire field by such cultural method.
Summary of the invention
The object of the invention is to solve fruit body of edible fungi that existing utilization section wood cultivates and there are residue of pesticide and the edible mushroom of this cultural method and cultivation thereof can not be used for the technical problem of viewing and admiring of lying fallow, a kind of cultural method of culture medium of edible fungus is provided.
For solving the problems of the technologies described above, the technical solution used in the present invention is as follows:
A cultural method for culture medium of edible fungus, wherein: it comprises the steps:
S1, to be put into by Duan Mu and cultivate box, and Duan Mu has buried by the mixture adding maize straw powder and quicklime in box, carry out a high-temperature sterilization to cultivating mixture in box, one time high-temperature sterilization temperature is 100 ~ 150 DEG C, and the time is 15 ~ 25min;
After S2, a sterilizing, in time cultivating box and be cooled to normal temperature, in cultivation box, inoculate food grade fungi strain;
Carry out cultural hypha to cultivation box after S3, inoculation, be covered with after cultivation box until mycelia and carry out two-stage sterilization, two-stage sterilization temperature is 60 ~ 80 DEG C, and the time is 30 ~ 45min;
S4, on the medium cultivating box, place filter, and at the outer plastic covering film of cultivation box.
In described S1, the mixture of maize straw powder and quicklime is mixed according to the mass ratio of 10:0.1 ~ 0.5 by maize straw powder and quicklime.
On described filter, the length of side of each lattice is 5 ~ 20mm.
Described cultivation box can cultivate Ganodermataceae edible mushroom, rainbow conk, Antrodia camphorata, grifola frondosus, mushroom, auricularia auriculajudae, white fungus or Phellinus.
When utilizing described cultivation box to cultivate fruit body, when mycelia is covered with filter, 1 ~ 3cm Nutrition Soil need be covered with thereon, Nutrition Soil be soil, ammonium chloride, peptone, dipotassium hydrogen phosphate and associated bacteria according to 10: 2.5 ~ 3.5: 3 ~ 4: 3 ~ 4: 0.01 ~ 0.1 mass ratio mix.
Owing to present invention employs technique scheme, compared with background technology, the invention solves the fruit body of edible fungi that the prior art section of utilization wood cultivates and there are residue of pesticide, and the edible mushroom of this cultural method and cultivation thereof can not be used for the technical problem of viewing and admiring.
The object of the medium plastic covering film that the present invention cultivates is isolate forster base, makes medium from the pollution of miscellaneous bacteria, earth, and from the invasion and attack of weeds, insect pest, the moisture of maintaining segment wood, covers one deck plastic foil as long as general and just can.If medium may be worn or puncture in carrying and incubation, then need which floor to cover, plastic foil does not have large space after covering medium, is conducive to the cultivation of fruit body of edible fungi more.
The medium that the present invention cultivates can depart from earth and cultivate, and frame high multilayer can not only cultivate, improves the availability in soil; And its fruit body of edible fungi cultivated is clean, pollution-free, people can cultivate fruit body of edible fungi at dust proof workshop, and the fruit body of edible fungi produced can directly be eaten raw; Can also carry out manual intervention to the temperature of medium, the whole year realizing fruit body of edible fungi cultivates.
The medium that the present invention cultivates can depart from earth and cultivate, and can realize the domestic of edible mushroom, and the incubation by edible mushroom introduces leisure area, and people can cultivate edible mushroom at home for the purpose of leisure.The fruit body of edible fungi cultivated also can be introduced leisure together with medium as potted landscape and view and admire field by people.
Embodiment
Below in conjunction with embodiment, the present invention is elaborated:
Embodiment 1
The cultural method of the culture medium of edible fungus in the present embodiment, it comprises the steps:
S1, to be put into by Duan Mu and cultivate box, and Duan Mu has buried by the mixture adding maize straw powder and quicklime in box, carry out a high-temperature sterilization to cultivating mixture in box, one time high-temperature sterilization temperature is 100 ~ 150 DEG C, and the time is 15 ~ 25min;
After S2, a sterilizing, in time cultivating box and be cooled to normal temperature, in cultivation box, inoculate food grade fungi strain;
Carry out cultural hypha to cultivation box after S3, inoculation, be covered with after cultivation box until mycelia and carry out two-stage sterilization, two-stage sterilization temperature is 60 ~ 80 DEG C, and the time is 30 ~ 45min;
S4, on the medium cultivating box, place filter, and at the outer plastic covering film of cultivation box.
In the present embodiment, in described S1, the mixture of maize straw powder and quicklime is mixed according to the mass ratio of 10:0.1 ~ 0.5 by maize straw powder and quicklime, and on described filter, the length of side of each lattice is 5 ~ 20mm.
Embodiment 2
The cultural method of the culture medium of edible fungus in the present embodiment, it comprises the steps:
S1, to be put into by Duan Mu and cultivate box, and Duan Mu has buried by the mixture adding maize straw powder and quicklime in box, carry out a high-temperature sterilization to cultivating mixture in box, one time high-temperature sterilization temperature is 100 DEG C, and the time is 25min;
After S2, a sterilizing, in time cultivating box and be cooled to normal temperature, in cultivation box, inoculate food grade fungi strain;
Carry out cultural hypha to cultivation box after S3, inoculation, be covered with after cultivation box until mycelia and carry out two-stage sterilization, two-stage sterilization temperature is 60 DEG C, and the time is 45min;
S4, on the medium cultivating box, place filter, and at the outer plastic covering film of cultivation box.
In the present embodiment, in described S1, the mixture of maize straw powder and quicklime is mixed according to the mass ratio of 10:0.1 by maize straw powder and quicklime, and on described filter, the length of side of each lattice is 5mm.
Embodiment 3
The cultural method of the culture medium of edible fungus in the present embodiment, it comprises the steps:
S1, to be put into by Duan Mu and cultivate box, and Duan Mu has buried by the mixture adding maize straw powder and quicklime in box, carry out a high-temperature sterilization to cultivating mixture in box, one time high-temperature sterilization temperature is 150 DEG C, and the time is 15min;
After S2, a sterilizing, in time cultivating box and be cooled to normal temperature, in cultivation box, inoculate food grade fungi strain;
Carry out cultural hypha to cultivation box after S3, inoculation, be covered with after cultivation box until mycelia and carry out two-stage sterilization, two-stage sterilization temperature is 80 DEG C, and the time is 30min;
S4, on the medium cultivating box, place filter, and at the outer plastic covering film of cultivation box.
In the present embodiment, in described S1, the mixture of maize straw powder and quicklime is mixed according to the mass ratio of 10:0.5 by maize straw powder and quicklime, and on described filter, the length of side of each lattice is 20mm.
Embodiment 4
The cultural method of the culture medium of edible fungus in the present embodiment, it comprises the steps:
S1, to be put into by Duan Mu and cultivate box, and Duan Mu has buried by the mixture adding maize straw powder and quicklime in box, carry out a high-temperature sterilization to cultivating mixture in box, one time high-temperature sterilization temperature is 125 DEG C, and the time is 20min;
After S2, a sterilizing, in time cultivating box and be cooled to normal temperature, in cultivation box, inoculate food grade fungi strain;
Carry out cultural hypha to cultivation box after S3, inoculation, be covered with after cultivation box until mycelia and carry out two-stage sterilization, two-stage sterilization temperature is 70 DEG C, and the time is 37min;
S4, on the medium cultivating box, place filter, and at the outer plastic covering film of cultivation box.
In the present embodiment, in described S1, the mixture of maize straw powder and quicklime is mixed according to the mass ratio of 10:0.3 by maize straw powder and quicklime, and on described filter, the length of side of each lattice is 13mm.
Utilize to cultivate described in above-described embodiment box cultivate fruit body of edible fungi time, when mycelia is covered with filter, 1cm Nutrition Soil need be covered with thereon, described Nutrition Soil be soil, ammonium chloride, peptone, dipotassium hydrogen phosphate and associated bacteria according to 10: 2.5: 3: 3: 0.01 mass ratio mix.
Utilize to cultivate described in above-described embodiment box cultivate fruit body of edible fungi time, when mycelia is covered with filter, 3cm Nutrition Soil need be covered with thereon, described Nutrition Soil be soil, ammonium chloride, peptone, dipotassium hydrogen phosphate and associated bacteria according to 10: 3.5: 4: 4: 0.1 mass ratio mix.
Utilize to cultivate described in above-described embodiment box cultivate fruit body of edible fungi time, when mycelia is covered with filter, 2cm Nutrition Soil need be covered with thereon, described Nutrition Soil be soil, ammonium chloride, peptone, dipotassium hydrogen phosphate and associated bacteria according to 10: 3: 3.5: 3.5: 0.06 mass ratio mix.
Cultivate box described in above-described embodiment and can cultivate Ganodermataceae edible mushroom, rainbow conk, Antrodia camphorata, grifola frondosus, mushroom, auricularia auriculajudae, white fungus or Phellinus.
Claims (4)
1. a cultural method for culture medium of edible fungus, is characterized in that: it comprises the steps:
S1, to be put into by Duan Mu and cultivate box, and Duan Mu has buried by the mixture adding maize straw powder and quicklime in box, carry out a high-temperature sterilization to cultivating mixture in box, one time high-temperature sterilization temperature is 100 ~ 150 DEG C, and the time is 15 ~ 25min;
After S2, a sterilizing, in time cultivating box and be cooled to normal temperature, in cultivation box, inoculate food grade fungi strain;
Carry out cultural hypha to cultivation box after S3, inoculation, be covered with after cultivation box until mycelia and carry out two-stage sterilization, two-stage sterilization temperature is 60 ~ 80 DEG C, and the time is 30 ~ 45min;
S4, on the medium cultivating box, place filter, and at the outer plastic covering film of cultivation box.
2. the cultural method of culture medium of edible fungus according to claim 1, is characterized in that: in described S1, the mixture of maize straw powder and quicklime is mixed according to the mass ratio of 10:0.1 ~ 0.5 by maize straw powder and quicklime.
3. the cultural method of culture medium of edible fungus according to claim 1, is characterized in that: on described filter, the length of side of each lattice is 5 ~ 20mm.
4. according to the cultural method of culture medium of edible fungus according to claim 1, it is characterized in that: described cultivation box can cultivate Ganodermataceae edible mushroom, rainbow conk, Antrodia camphorata, grifola frondosus, mushroom, auricularia auriculajudae, white fungus or Phellinus.
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| CN201510632170.6A CN105123275A (en) | 2015-09-29 | 2015-09-29 | Culture method for edible fungus culture medium |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107484555A (en) * | 2017-09-29 | 2017-12-19 | 遵义晨光云农业产业发展有限公司 | A kind of implantation methods for setting ear |
| CN107637396A (en) * | 2017-10-16 | 2018-01-30 | 宁夏田媛菌草有限公司 | A kind of inoculation method of edible mushroom bacterium bag |
| CN109168978A (en) * | 2018-11-14 | 2019-01-11 | 遵义三湾田种植有限公司 | A kind of cultural method of culture medium of edible fungus |
| CN109699392A (en) * | 2019-01-25 | 2019-05-03 | 四川省农业科学院土壤肥料研究所 | A method of using clinker section wood successively cultivation tremella and ganoderma lucidum |
| CN110476720A (en) * | 2019-09-10 | 2019-11-22 | 安顺学院 | A kind of bacterium bag producing method for seed of winter sweet-smelling grass and dictyophora phalloidea |
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| US3996038A (en) * | 1971-05-27 | 1976-12-07 | Licencia Talalmanyokat Ertekesito Vallalat | Mushroom growing process |
| CN101960962A (en) * | 2010-10-26 | 2011-02-02 | 镇江市京口区瑞京农业科技示范园 | Method for culturing bamboo fungi by using maize straws |
| CN102523936A (en) * | 2012-03-10 | 2012-07-04 | 潘启城 | Method for cultivating bamboo shoots in open air by utilizing banana stalks and stem leaves as base materials |
| CN103238463A (en) * | 2013-04-29 | 2013-08-14 | 何松庆 | Edible mushroom culture medium, manufacturing method thereof and edible mushroom culture method |
| CN103238468A (en) * | 2013-05-24 | 2013-08-14 | 常熟市润丰农业有限公司 | High-yield cultivation method of pollution-free straw mushrooms |
| CN103314783A (en) * | 2013-06-12 | 2013-09-25 | 邬金飞 | Ordinary pressure intermittent sterilization method of production of wheat cultures of edible fungus |
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2015
- 2015-09-29 CN CN201510632170.6A patent/CN105123275A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3996038A (en) * | 1971-05-27 | 1976-12-07 | Licencia Talalmanyokat Ertekesito Vallalat | Mushroom growing process |
| CN101960962A (en) * | 2010-10-26 | 2011-02-02 | 镇江市京口区瑞京农业科技示范园 | Method for culturing bamboo fungi by using maize straws |
| CN102523936A (en) * | 2012-03-10 | 2012-07-04 | 潘启城 | Method for cultivating bamboo shoots in open air by utilizing banana stalks and stem leaves as base materials |
| CN103238463A (en) * | 2013-04-29 | 2013-08-14 | 何松庆 | Edible mushroom culture medium, manufacturing method thereof and edible mushroom culture method |
| CN103238468A (en) * | 2013-05-24 | 2013-08-14 | 常熟市润丰农业有限公司 | High-yield cultivation method of pollution-free straw mushrooms |
| CN103314783A (en) * | 2013-06-12 | 2013-09-25 | 邬金飞 | Ordinary pressure intermittent sterilization method of production of wheat cultures of edible fungus |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107484555A (en) * | 2017-09-29 | 2017-12-19 | 遵义晨光云农业产业发展有限公司 | A kind of implantation methods for setting ear |
| CN107637396A (en) * | 2017-10-16 | 2018-01-30 | 宁夏田媛菌草有限公司 | A kind of inoculation method of edible mushroom bacterium bag |
| CN109168978A (en) * | 2018-11-14 | 2019-01-11 | 遵义三湾田种植有限公司 | A kind of cultural method of culture medium of edible fungus |
| CN109699392A (en) * | 2019-01-25 | 2019-05-03 | 四川省农业科学院土壤肥料研究所 | A method of using clinker section wood successively cultivation tremella and ganoderma lucidum |
| CN110476720A (en) * | 2019-09-10 | 2019-11-22 | 安顺学院 | A kind of bacterium bag producing method for seed of winter sweet-smelling grass and dictyophora phalloidea |
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Application publication date: 20151209 |