CN105105253A - Making method for rose ferment health-care beverage - Google Patents

Making method for rose ferment health-care beverage Download PDF

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Publication number
CN105105253A
CN105105253A CN201510275233.7A CN201510275233A CN105105253A CN 105105253 A CN105105253 A CN 105105253A CN 201510275233 A CN201510275233 A CN 201510275233A CN 105105253 A CN105105253 A CN 105105253A
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China
Prior art keywords
rose
fermentation
ferment
enzyme beverage
beverage
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Pending
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CN201510275233.7A
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Chinese (zh)
Inventor
廖祥儒
孙萌
李韵雅
李云
蔡宇杰
管政兵
刘海燕
明经林
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Jiangnan University
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Jiangnan University
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Priority to CN201510275233.7A priority Critical patent/CN105105253A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38Other non-alcoholic beverages
    • A23L2/382Other non-alcoholic beverages fermented
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus

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  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The invention provides a method for preparing a ferment beverage from rose. According to the invention, rose is used as a main fermentation raw material, green molasses and Bainian Shangshui provided by Bainian Shangshui Beverage Limited Company, Wuxi, Jiangsu Province are added, and a fermentation stock solution is prepared from rose, green molasses and Bainian Shangshui according to a volume ratio of 2-5: 1-3: 7-2; nectar-source fungi and lactic acid bacteria are used as fermentation strains, and 1 to 5% of nectar-source fungi and 1 to 5% of lactic acid bacteria are added into the fermentation stock solution; and fermentation is carried out at 25 to 35 DEG C in an environment with a pH value of 5.0 to 8.0 for about 10 d, so the rose ferment beverage is prepared. According to the invention, complete fermentation is realized, so a plurality of enzymes, vitamins, amino acids and trace elements in the raw materials are fully released; the produced ferment beverage has flavor unique to rose and is rich in nutrients and tasty; and the method employs traditional fermentation technology and belongs to the technical field of bioengineering.

Description

A kind of preparation method of rose ferment health drink
Technical field
The present invention relates to the production method of flower ferment, especially the fermentation process of rose fresh flowers ferment health drink, belongs to technical field of bioengineering.
Background technology
Ferment originates from a kind of call of Japanese to enzyme (enzyme), and Japan and TaiWan, China were referred to as ferment the initial bio-active products obtained through fermentable afterwards.But the many concerns of producer add different fruit, do not note the use of fermentative microorganism and wild functional material, ferment product development and application are subject to its side effect and functional not strong impact.Although fruit ferment is known as toxin expelling, beauty treatment, filling blood three acts on greatly; The ferment of current use includes more than the 1000 kind of compound that trypsase, chymotrypsin, lipase and amylase etc. are combined to form, this fermentoid is by the decomposition to five large nutrients, and reach increase absorption, nurse one's health, menstruation regulating is anti-ageing waits comprehensive function comprehensively.But effect of ferment also depends on bacterial classification and fermentation main material.
Rose and multiflora rose (RosamultifloraThunb.) are rose family guls.Multiflora rose is climbing shrub; Sprig is cylindrical, usually without hair, has short, thick slightly bending skin bundle.Leaflet 5-9, the leaflet of nearly inflorescence sometimes 3, connects the long 5-10 centimetre of petiole; Vanelets obovate, Long Circle or avette, long 1.5-5 centimetre, wide 8-28 millimeter, the anxious point of tip or circle blunt, base portion subcircular or wedge shape, there is sharp-pointed single sawtooth at edge, is rarely mixed with heavy sawtooth, above without hair, have pubescence below.Petiolule and rachis have pubescence or without hair, have scattered glandular hairs; Stipule comb dentation, large portion adhesion is in petiole, and edge has or without glandular hairs.Hua Duoduo, lines up coniform inflorescence, the long 1.5-2.5 centimetre of bennet, and without hair or there are glandular hairs, base portion has comb dentation squamella sometimes; Flower diameter 1.5-2 centimetre, sepal lanceolar, sometimes middle part tool 2 linear slivers, outside is without hair, and inner face has pubescence; Petal white, wide obovate, tip nick, base portion wedge shape; Style is in conjunction with bunchy, without hair, slightly longer than stamen.Fruit is subsphaeroidal, and diameter 6-8 millimeter, bronzing or puce, glossy, without hair, sepal comes off.Multiflora rose property is strong, happiness light, resistance to half cloudy, cold-resistant, soil is required tight, also can normal growth in heavy soil.Barren-resistant, avoid low-lying ponding.Best with fertile, loose subacidity soil.Multiflora rose originates in China, is distributed in North China, Central China, East China, south China and southwest, and the Plain of each provinces and regions and low mountains and hills on the south the main product Huanghe valley, kind is a lot of, and house booth garden is common.
Rose is the flower of machaka multiflora rose, and rose has another name called white residual flower, thorn Mi, buys and laugh at, and is exactly good colored name grass from ancient times.Rose taste is sweet, sour, cool in nature, enters spleen, lung, large intestine.Rose has effect of clearing away summer-heat and eliminating dampness, pleasant and stomach, hemostasis.Be applicable to that treatment hot summer weather is uncomfortable in chest, thirsty, vomiting, mouth kept silent, suffered from diarrhoea, dysentery, haematemesis and traumatism and bleeding etc.There is certain curative effect to dysentery, uncomfortable in chest, heatstroke, still treat the good medicine of apocleisis, aphthous ulcer simultaneously.But it is not easily fresh-keeping, seasonal strong.Conventional machining techniques easily loses its active ingredient, limits the application of rose.This technology with fresh rose for main material, probio in the microorganism of application nectar source and lactic acid bacteria class fermentation are for ferment, not only originally function and the local flavor of fresh rose will be kept, also the benefit materials of the anti-sex pheromone that its probio produces will be given, there is provided the various prebioticses of probio, for the healthy of consumer provides new functional product.
The deposit number that this patent is stored in Wuhan University's China typical culture collection center with on January 7th, 2015 is: the high ground bacillus of the Bacillussp.SYBChb4 of CCTCCM2015018 is for fermentation strain.
Summary of the invention
The invention provides the preparation method that a kind of mouthfeel is good, can keep rose ferment nutritive and health protection components to greatest extent, solve in existing ferment production process the pollution that can not ensure without pathogen, fermentation time is long, and the problem such as effectively enzyme activity cannot to be retained for a long time.
For solving the problems of the technologies described above, the technical solution used in the present invention is:
The fermentation process of rose ferment health drink, comprises the following steps:
(1) selected material: select the rose completely open, fresh and alive, the natural ferment content of color and luster is high for fermentation main material.
(2) process of material: first use tap water rose surface, re-use a century that Wuxi century-old Shang Shui drink Co., Ltd provides still water clear water wash twice, finally soak 120 seconds by the NaCl solution of 0.9% again, drain for subsequent use.
(3) Spawn incubation: a century using Wuxi century-old Shang Shui drink Co., Ltd to provide still water preparation LB culture medium, by former preservation bacterial strain activation, cultivates the bacterium liquid obtained for about 48 hours for subsequent use.
(4) inoculation fermentation: all according to inoculative proportion 1 ~ 5%, by nectar source bacterium and lactic acid bacteria access fermenation raw liquid, each bacteria concentration is 10 6cFU/ml, fermentation 6 ~ 14d, pH value 5.0 ~ 8.0, temperature 25 DEG C ~ 35 DEG C.
(5) post-processed makes: coarse filtration, tentatively screens out bulky grain residue, avoids putting for a long time easily to cause becoming sour, filter condition: sieves number is 20 ~ 32 orders.Thin filtration: filter small particle residue further, makes liquid state present limpider state, filter condition: sieves number is 80 ~ 100 orders, obtains natural rose ferment drink.
In step (1), the preparation method of rose ferment raw material comprises the steps:
(11) instrument used, utensil all want sterilizing, without greasy.
(12), after rose is clean with pure water, drain with the saline sook of pure water or 0.9%.
(13) again rose is placed in sterilized container, after add the century-old Shang Shui that green molasses and Wuxi century-old Shang Shui drink Co., Ltd provides, wherein volume ratio rose in fermenation raw liquid: green molasses: pure water=2 ~ 5: 1 ~ 3: 7 ~ 2, obtains rose fermenation raw liquid.
Beneficial effect of the present invention:
1. utilize cold sterilization, strictly control fermentation temperature, effectively reduce the loss of ferment nutritional labeling, remain enzyme activity to greatest extent.
2. accessed nectar source bacterium and lactic acid bacteria, dominant strain inhibits other miscellaneous bacterias to grow, and makes the steady quality of product.
3. the total time of fermentation is about 10d, and the time is short to be avoided polyoxygenated, was conducive to the ferment material keeping rose itself to contain, and the flavor substance itself contained.
Detailed description of the invention
Below in conjunction with the embodiment of the present invention, carry out clear, perfect description to technical scheme of the present invention, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, other embodiments that those of ordinary skill in the art obtain under the prerequisite of not making creative work, all belong to the scope of protection of the invention.
Embodiment 1
To ferment the method for rose ferment, comprise the following steps:
(1) select the rose completely open, fresh and alive, the natural ferment content of color and luster is high as fermentation primary raw material.
(2) pure water clear water washes twice, and finally soak by the NaCl solution of 0.9%, cold sterilization, then drains again, and load in the cleaned container of pure water, probably account for 30% of volume, adding the green molasses accounting for total liquid volume 5% is that fermenation raw liquid is for subsequent use.
(3) prepare LB culture medium, by former preservation bacterial strain activation, cultivate the zymocyte liquid obtained for about 48 hours for subsequent use.
(4) all according to inoculative proportion 5%, nectar source bacterium, Lactobacillus casei and Lactobacillus plantarum are got access fermenation raw liquid respectively, fermentation 7d, pH value 7.0, temperature 25 DEG C.
(5) first carry out coarse filtration, sieves number is 20 ~ 32 orders.Then carefully filter, sieves number, for being 80 ~ 100 orders, obtains the natural rose ferment of clarification.
Embodiment 2
To ferment the method for rose ferment, comprise the following steps:
(1) select the rose completely open, fresh and alive, the natural ferment content of color and luster is high as fermentation primary raw material.
(2) pure water clear water washes twice, and finally soak by the NaCl solution of 0.9%, cold sterilization, then drains again, and load in the cleaned container of pure water, probably account for 40% of volume, adding the green molasses accounting for total liquid volume 10% is that fermenation raw liquid is for subsequent use.
(3) prepare LB culture medium, by former preservation bacterial strain activation, cultivate the zymocyte liquid obtained for about 48 hours for subsequent use.
(4) all according to inoculative proportion 4%, nectar source bacterium, Lactobacillus casei and Lactobacillus plantarum are got access fermenation raw liquid respectively, fermentation 7d, pH value 7.0, temperature 25 DEG C.
(5) first carry out coarse filtration, sieves number is 20 ~ 32 orders.Then carefully filter, sieves number, for being 80 ~ 100 orders, obtains the natural rose ferment of clarification.
Embodiment 3
To ferment the method for rose ferment, comprise the following steps:
(1) select the rose completely open, fresh and alive, the natural ferment content of color and luster is high as fermentation primary raw material.
(2) pure water clear water washes twice, and finally soak by the NaCl solution of 0.9%, cold sterilization, then drains again, and load in the cleaned container of pure water, probably account for 50% of volume, adding the green molasses accounting for total liquid volume 15% is that fermenation raw liquid is for subsequent use.
(3) prepare LB culture medium, by former preservation bacterial strain activation, cultivate the zymocyte liquid obtained for about 48 hours for subsequent use.
(4) all according to inoculative proportion 3%, nectar source bacterium, Lactobacillus casei and Lactobacillus plantarum are got access fermenation raw liquid respectively, fermentation 7d, pH value 7.0, temperature 25 DEG C.
(5) first carry out coarse filtration, sieves number is 20 ~ 32 orders.Then carefully filter, sieves number, for being 80 ~ 100 orders, obtains the natural rose ferment of clarification.
Rose fresh flowers in the present invention contains abundant natural ferment material and the element useful to human body, but be not limited only to this, can according to actual conditions, determine that the position of rose and amount or the vegetable material selecting other suitable mix, as long as ferment reasonable mixture ratio can be reached, ferment content higher position is passable.
In addition the selection of fermenation raw liquid and proportioning, connect bacterium amount and be also not limited to the combination that embodiment lifts, different combinations can be selected according to actual conditions.
The foregoing is only preferred embodiments of the present invention, not in order to limit the present invention, all within thinking of the present invention and principle, any amendment made, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.

Claims (7)

1. a preparation method for rose ferment health drink, is characterized in utilizing rose for dominant fermentation raw material, adds green molasses, and adopt nectar source bacterium, lactic acid bacteria, manufacture rose enzyme beverage, its technique is:
1) the rose running water completely open, color and luster is fresh and alive is selected to clean, re-use a century that Wuxi century-old Shang Shui drink Co., Ltd provides still water clear water wash twice, finally invade bubble 120 seconds by the NaCl solution of 0.9% again, drain, put into tank, volume ratio is by rose: green molasses: pure water=2 ~ 5: 1 ~ 3: 7 ~ 2 are mixed with fermenation raw liquid;
2) actication of culture of nectar source bacterium and lactic acid bacteria is prepared into zymocyte liquid:
(1) get glycerine pipe and preserve bacterial classification one ring at flat lining out, cultivate 24 hours for 30 DEG C in incubator;
(2) get LB solid medium flat-plate bacterial colony one articulating to enter to be equipped with in the triangular flask of 250ml specification of 50ml seed culture fluid, prepare some parts, be placed in shaking table, condition of culture: rotating speed 200rpm, temperature 30 DEG C, constant temperature culture 24 hours, is fermentation seed bacterium liquid;
(3) seed culture fluid preparation: peptone 10g, yeast extract 5g, sodium chloride 10g, constant volume, to 1L, adjusts pH=7.0;
3) join in fermentation system in 1 ~ 5% ratio of fermenation raw liquid volume by zymocyte liquid, fermentation temperature is 25 ~ 35 DEG C, fermentation time 6 ~ 14d;
4) by deployed rose enzyme beverage, through screen number, to be 20 ~ 32 object coarse filtrations and sieves number be that 80 ~ 100 objects carefully filter removes large and small granular residue respectively to obtain natural rose ferment drink.
2. rose according to claim 1 prepares the method for enzyme beverage, it is characterized in that: the rose that have employed certain medical value, and nectar source bacterium refers to the edible bacillus of sieving from natural honey simultaneously.
3. fermentation according to claim 1 is for the method for rose enzyme beverage, it is characterized in that: the deposit number that fermentation strain adopts on January 7th, 2015 to be stored in China typical culture collection center is the Bacillus amyloliquefaciens strain of the Bacillussp.SYBChb4 of CCTCCM2015018 is fermentation strain.
4. fermentation according to claim 1 is for the method for rose enzyme beverage, it is characterized in that: the addition of natural honey is 10%-30%.
5. fermentation according to claim 1 is for the method for rose enzyme beverage, it is characterized in that: rose addition is 20%-50%.
6. fermentation according to claim 1 is for the method for rose enzyme beverage, it is characterized in that: water refers to the century-old Shang Shui that Wuxi century-old Shang Shui drink Co., Ltd provides, and addition is 20%-70%.
7. fermentation according to claim 1 is for the method for rose enzyme beverage, it is characterized in that: described lactic acid bacteria refers to Lactobacillus casei and the Lactobacillus plantarum of commodity production, and addition is the 1%-5% of cumulative volume.
CN201510275233.7A 2015-05-25 2015-05-25 Making method for rose ferment health-care beverage Pending CN105105253A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109247474A (en) * 2018-08-28 2019-01-22 厦门和美科盛生物技术有限公司 Application of one lactobacillus plantarum in preparation lactic acid bacteria rose fermented beverage

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2592158A1 (en) * 2011-11-14 2013-05-15 University College Cork, National University of Ireland, Cork Lactobacillus plantarum species possessing broad spectrum anti-fungal activity and exhibiting high heat tolerance and osmotolerance
CN103451128A (en) * 2013-07-12 2013-12-18 江南大学 Bacillus amyloliquefaciens and application thereof in preventing and controlling peach bleeding disease
CN103609985A (en) * 2013-10-25 2014-03-05 晶叶(青岛)生物科技有限公司 Fermentation method for production of nutritionally balanced natural fruit enzyme
CN104489669A (en) * 2014-12-12 2015-04-08 深圳先进技术研究院 Plant enzyme probiotic powder and preparation method thereof
CN104522557A (en) * 2014-12-15 2015-04-22 深圳先进技术研究院 Sugar-free ferment and preparation method thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2592158A1 (en) * 2011-11-14 2013-05-15 University College Cork, National University of Ireland, Cork Lactobacillus plantarum species possessing broad spectrum anti-fungal activity and exhibiting high heat tolerance and osmotolerance
CN103451128A (en) * 2013-07-12 2013-12-18 江南大学 Bacillus amyloliquefaciens and application thereof in preventing and controlling peach bleeding disease
CN103609985A (en) * 2013-10-25 2014-03-05 晶叶(青岛)生物科技有限公司 Fermentation method for production of nutritionally balanced natural fruit enzyme
CN104489669A (en) * 2014-12-12 2015-04-08 深圳先进技术研究院 Plant enzyme probiotic powder and preparation method thereof
CN104522557A (en) * 2014-12-15 2015-04-22 深圳先进技术研究院 Sugar-free ferment and preparation method thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109247474A (en) * 2018-08-28 2019-01-22 厦门和美科盛生物技术有限公司 Application of one lactobacillus plantarum in preparation lactic acid bacteria rose fermented beverage
CN109247474B (en) * 2018-08-28 2021-12-24 厦门和美科盛生物技术有限公司 Application of lactobacillus plantarum in preparation of lactobacillus rose fermented beverage

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Application publication date: 20151202