CN105087668A - Method for preparing (S)-fluoro-phenyl ethanol with penicillium - Google Patents
Method for preparing (S)-fluoro-phenyl ethanol with penicillium Download PDFInfo
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- 241000228143 Penicillium Species 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims abstract description 12
- NOBXCSGDCRMGSD-MRVPVSSYSA-N (1S)-1-fluoro-1-phenylethanol Chemical compound F[C@](C)(O)C1=CC=CC=C1 NOBXCSGDCRMGSD-MRVPVSSYSA-N 0.000 title 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N EtOH Substances CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 46
- 238000006243 chemical reaction Methods 0.000 claims abstract description 39
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 36
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 22
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 22
- 239000000243 solution Substances 0.000 claims description 22
- 230000002210 biocatalytic effect Effects 0.000 claims description 18
- 240000008042 Zea mays Species 0.000 claims description 17
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 17
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 17
- 235000005822 corn Nutrition 0.000 claims description 17
- 238000009423 ventilation Methods 0.000 claims description 17
- 241000228150 Penicillium chrysogenum Species 0.000 claims description 16
- 235000015097 nutrients Nutrition 0.000 claims description 16
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 14
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 12
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 12
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 12
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 12
- 229940041514 candida albicans extract Drugs 0.000 claims description 12
- 239000008103 glucose Substances 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 12
- 239000012138 yeast extract Substances 0.000 claims description 12
- 241000790917 Dioxys <bee> Species 0.000 claims description 11
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 11
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 11
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 11
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 11
- 239000011591 potassium Substances 0.000 claims description 11
- 229910052700 potassium Inorganic materials 0.000 claims description 11
- 238000002360 preparation method Methods 0.000 claims description 11
- 239000000758 substrate Substances 0.000 claims description 11
- HCEKGPAHZCYRBZ-UHFFFAOYSA-N 1-(3-fluorophenyl)ethanone Chemical compound CC(=O)C1=CC=CC(F)=C1 HCEKGPAHZCYRBZ-UHFFFAOYSA-N 0.000 claims description 8
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims description 7
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 7
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims description 7
- 229930006000 Sucrose Natural products 0.000 claims description 7
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 7
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 7
- 239000011790 ferrous sulphate Substances 0.000 claims description 7
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 7
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 7
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 7
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 7
- 230000035484 reaction time Effects 0.000 claims description 7
- 239000005720 sucrose Substances 0.000 claims description 7
- XZIIFPSPUDAGJM-UHFFFAOYSA-N 6-chloro-2-n,2-n-diethylpyrimidine-2,4-diamine Chemical compound CCN(CC)C1=NC(N)=CC(Cl)=N1 XZIIFPSPUDAGJM-UHFFFAOYSA-N 0.000 claims description 6
- 239000000872 buffer Substances 0.000 claims description 6
- 208000012839 conversion disease Diseases 0.000 claims description 6
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- 239000002953 phosphate buffered saline Substances 0.000 claims description 6
- 229940035044 sorbitan monolaurate Drugs 0.000 claims description 6
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 238000007796 conventional method Methods 0.000 abstract 1
- 239000008055 phosphate buffer solution Substances 0.000 abstract 1
- 238000006555 catalytic reaction Methods 0.000 description 7
- 239000005515 coenzyme Substances 0.000 description 7
- 239000002609 medium Substances 0.000 description 7
- 229920001817 Agar Polymers 0.000 description 6
- 239000008272 agar Substances 0.000 description 6
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- 239000001963 growth medium Substances 0.000 description 5
- 238000011218 seed culture Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 238000012807 shake-flask culturing Methods 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 3
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- 230000003197 catalytic effect Effects 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
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- 241000024287 Areas Species 0.000 description 1
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- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000011914 asymmetric synthesis Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
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- 230000003287 optical effect Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000012429 reaction media Substances 0.000 description 1
- 238000006479 redox reaction Methods 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
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- 238000011069 regeneration method Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
A method for preparing (S)-3'-fluoro-phenyl ethanol through biocatalysis with penicillium cells comprises the following steps: adding a phosphate buffer solution into a reaction tank; carrying out biocatalysis with the penicillium cells. The method has the advantages that the product yield is high; the enantiomeric excess rate is high; the problem of difficult production caused by adoption of the conventional method is solved.
Description
Technical field
The invention belongs to technology of pharmaceutical engineering field, be related specifically to Penicillium cell biocatalysis and prepare chiral medicinal intermediate (S)-3 ' technology of-fluorobenzene ethanol.
Background technology
Chirality (S)-3 '-fluorobenzene ethanol is the important intermediate of synthesis of chiral medicine, fine chemicals, agricultural chemicals product and other exotic materialss.Biocatalysis asymmetric reaction has environmental friendliness, mild condition, selectivity advantages of higher, prepares the first approach of chipal compounds as green high-efficient, is applied to the chipal compounds producing some high added values more and more.Biocatalysis prepares chirality (S)-3 '-fluorobenzene ethanol has good application prospect.
Biocatalysis has the outstanding advantages such as catalytic efficiency is high, selectivity strong, mild condition, environmental friendliness, is the important method substituting and expand traditional organic chemical synthesis in process of sustainable development.Wherein chiral separation and asymmetric synthesis are the Application Areass of biocatalysis most magnetism.As in six large fermentoids of biological catalyst, lytic enzyme catalytic kinetics resolution of racemates can obtain quiral products, in industrial biocatalytic, play key player always.In recent years, oxydo-reductase application industrially obtained and increased rapidly.At present, the ratio adopting the industry of lytic enzyme Kinetic Resolution, biological reducing method and biological oxidation process to prepare optical activity chirality compound is 4:2:1.
Biological reducing method is for Kinetic Resolution, and maximum advantage is that theoretical yield can reach 100%, and Atom economy is good.But bioreduction needs the participation of coenzyme or cofactor, limit its application to a certain extent.Due to the dependent cause of coenzyme, in bioreduction, many Bian intact cells are as catalyzer, realize the purification procedures eliminating enzyme in body while coenzyme cyclic regeneration.
(S)-3 '-fluorobenzene ethanol is the important chiral building block of synthesis of chiral medicine, fine chemicals, agricultural chemicals product.Existing method ubiquity low conversion rate, long reaction time, needs add expensive coenzyme, high in cost of production major defect, are difficult to carry out suitability for industrialized production.The present invention will adopt biomass cells catalysis to prepare (S)-3 '-fluorobenzene ethanol.
Summary of the invention
The present invention adopts Penicillium cell catalysis to prepare (S)-3 '-fluorobenzene ethanol, and reaction formula is as follows:
3 '-fluoro acetophenone (1), through Penicillium notatum catalyzed reaction, obtains product (S)-3 '-fluorobenzene ethanol (2).Have multiple-microorganism can catalysis this reaction, through great many of experiments screening, finally determine adopt Penicillium notatum as catalyzer because the effect of its catalyzed reaction is best, reaction yield, enantiomeric excess rate (ee%) are all very high.
Many Penicillium notatums can carry out this reaction of biocatalysis, but its effect is different, differs greatly, and through experiment, the present invention selects Penicillium strain to be ATCC204052, its catalyzed reaction best results.
Because most oxydo-reductase is coenzyme NAD (P) H dependent form, and the coenzyme amount contained by cell itself may be less, therefore, when carrying out redox reaction, for promoting regenerating coenzyme, improving reaction efficiency, in reaction system, also adding cosubstrate form transformation system.Conventional cosubstrate kind is more, and the cosubstrate needed for different cell is different, and effect difference is very large, adopts several cosubstrate to combine, can obtain better effects, need carry out great many of experiments and could determine best cosubstrate.The cosubstrate that the present invention determines through great many of experiments is: sucrose 25-28g/L, maltose 33-36g/L, Virahol 5-6%V/V (volumetric concentration).
Because substrate solubility is lower, so, add tensio-active agent to increase the solubleness of substrate.
developing medium:
1, seed culture medium composition is: yeast extract 10g/L, glucose 20g/L, peptone 20g/L, malt extract 25-30g, ammonium sulfate 0.5-0.7g/L; The agar powder of 15g/L is added during preparation solid medium.
2, nutrient solution composition: corn steep liquor (with dry basis) content is 35-45g/L; yeast extract 8-10g/L; glucose 18-22g/L; malt extract 25-30g, ammonium sulfate 0.5-0.7g/L, magnesium sulfate 0.3-0.4g/L; phosphoric acid dioxy potassium 1.8-2.3g/L; calcium carbonate 0.7g/L, ferrous sulfate 0.18g/L, manganous sulfate 0.025g/L; PH5.0.
2, solid medium composition: add the agar powder of 2% in liquid medium within.
Prepared by Penicillium cell.Penicillium notatum through inclined-plane, shaking flask, seed tank culture obtain seed liquor; Fermentor tank adds nutrient solution, 121 DEG C of autoclavings 30 minutes, be cooled to 23-24 DEG C, Penicillium notatum seed liquor is seeded to fermentor tank, inoculative proportion is 10-11%, and ventilation ratio is 0.9-1V/(V minute), cultivate 35-40 hour for 23-24 DEG C, wet Penicillium cell is obtained, as biocatalytic reaction catalyzer with filtering centrifuge is centrifugal.
Biocatalytic Conversion.Add phosphate buffered saline buffer in bottom ventilation stirred tank, pH is 5.2, adds substrate 3 '-fluoro acetophenone, makes concentration of substrate be 80-90g/L.Other compositions: corn steep liquor (with dry basis) content is 12-15g/L, sorbitan monolaurate 17-18g/L, sucrose 25-28g/L, maltose 33-36g/L, Virahol 5-6%V/V (volumetric concentration), 121 DEG C of autoclavings 30 minutes; When being cooled to 30-31 DEG C, add wet Penicillium cell and make its concentration be 66-70g/L, ventilation ratio is 0.1-0.11V/(V minute), carry out biocatalytic reaction, the reaction times is 66-69 hour; After reaction terminates, leach cell, be extracted with ethyl acetate reaction solution, steam ethyl acetate, obtain product (S)-3 '-fluorobenzene ethanol, reaction conversion ratio 98-99%, product yield 96-97%, enantiomeric excess rate 98.5-99%.
The present invention carries out the work and comprises bacterial strain selection, catalytic reaction condition optimization (temperature of reaction, air flow, pH), reaction medium selection and concentration optimization, comprises concentration of substrate, cosubstrate combination, kinds of surfactants and concentration etc.
embodiment 1
Seed culture medium composition is: corn steep liquor (with dry basis) content is 35-45g/L, yeast extract 10g/L, glucose 20g/L, ammonium sulfate 0.5-0.7g/L, magnesium sulfate 0.3-0.4g/L, phosphoric acid dioxy potassium 1.8-2.3g/L; The agar powder of 15g/L is added during preparation solid medium.
Corn steep liquor (with dry basis) content is 35-45g/L; yeast extract 8-10g/L; glucose 18-22g/L; malt extract 25-30g, ammonium sulfate 0.5-0.7g/L, magnesium sulfate 0.3-0.4g/L; phosphoric acid dioxy potassium 1.8-2.3g/L; calcium carbonate 0.7g/L, ferrous sulfate 0.18g/L, manganous sulfate 0.025g/L; PH5.0.
Nutrient solution composition is: corn steep liquor (with dry basis) content is 35-45g/L; yeast extract 8-10g/L; glucose 18-22g/L; malt extract 25-30g, ammonium sulfate 0.5-0.7g/L, magnesium sulfate 0.3-0.4g/L; phosphoric acid dioxy potassium 1.8-2.3g/L; calcium carbonate 0.7g/L, ferrous sulfate 0.18g/L, manganous sulfate 0.025g/L; PH5.0.
Wet Penicillium cell preparation process is as follows: Penicillium notatum ATCC204052 through inclined-plane, shake-flask culture, obtain seed liquor.1L fermentor tank adds nutrient solution; Fermentor tank adds nutrient solution, 121 DEG C of autoclavings 30 minutes, be cooled to 23 DEG C, Penicillium notatum seed liquor is seeded to fermentor tank, inoculative proportion is inoculative proportion is 10-11%, and ventilation ratio is 0.9-1V/(V minute), cultivate 35-40 hour for 23 DEG C, wet Penicillium cell is obtained, as biocatalytic reaction catalyzer with filtering centrifuge is centrifugal.
Biocatalytic Conversion: add phosphate buffered saline buffer in 0.5L bottom ventilation stirred reactor, pH is 5.2, add substrate 3 '-fluoro acetophenone, its concentration is made to be 80g/L, corn steep liquor (with dry basis) content is 12g/L, sorbitan monolaurate 17g/L, sucrose 25g/L, maltose 36g/L, Virahol 6%V/V (volumetric concentration), 121 DEG C of autoclavings 30 minutes; When being cooled to 30 DEG C, add wet Penicillium cell and make its concentration be 66g/L, ventilation ratio is 0.1V/(V minute), carry out biocatalytic reaction, the reaction times is 66 hours; After reaction terminates, leach cell, be extracted with ethyl acetate reaction solution, steam ethyl acetate, obtain product (S)-3 '-fluorobenzene ethanol, reaction conversion ratio 99%, product yield 97%, enantiomeric excess rate 98.5%.
embodiment 2
Seed culture medium composition is: corn steep liquor (with dry basis) content is 35-45g/L, yeast extract 10g/L, glucose 20g/L, ammonium sulfate 0.5-0.7g/L, magnesium sulfate 0.3-0.4g/L, phosphoric acid dioxy potassium 1.8-2.3g/L; The agar powder of 15g/L is added during preparation solid medium.
Nutrient solution composition is: corn steep liquor (with dry basis) content is 35-45g/L; yeast extract 8-10g/L; glucose 18-22g/L; malt extract 25-30g, ammonium sulfate 0.5-0.7g/L, magnesium sulfate 0.3-0.4g/L; phosphoric acid dioxy potassium 1.8-2.3g/L; calcium carbonate 0.7g/L, ferrous sulfate 0.18g/L, manganous sulfate 0.025g/L; PH5.0.
Wet Penicillium cell preparation process is as follows: Penicillium notatum ATCC204052 through inclined-plane, shake-flask culture, obtain seed liquor.5L fermentor tank adds nutrient solution; Fermentor tank adds nutrient solution, 121 DEG C of autoclavings 30 minutes, be cooled to 24 DEG C, Penicillium notatum seed liquor is seeded to fermentor tank, inoculative proportion is 10-11%, and ventilation ratio is 0.9-1V/(V minute), cultivate 35-40 hour for 24 DEG C, wet Penicillium cell is obtained, as biocatalytic reaction catalyzer with filtering centrifuge is centrifugal.
Biocatalytic Conversion: add phosphate buffered saline buffer in 5L bottom ventilation stirred reactor, pH is 5.2, add substrate 3 '-fluoro acetophenone, its concentration is made to be 90g/L, corn steep liquor (with dry basis) content is 15g/L, sorbitan monolaurate 18g/L, sucrose 28g/L, maltose 33g/L, Virahol 5%V/V (volumetric concentration), 121 DEG C of autoclavings 30 minutes; When being cooled to 31 DEG C, add wet Penicillium cell and make its concentration be 70g/L, ventilation ratio is 0.11V/(V minute), carry out biocatalytic reaction, the reaction times is 69 hours; After reaction terminates, leach cell, be extracted with ethyl acetate reaction solution, steam ethyl acetate, obtain product (S)-3 '-fluorobenzene ethanol, reaction conversion ratio 98%, product yield 96%, enantiomeric excess rate 99%.
embodiment 3
Seed culture medium composition is: corn steep liquor (with dry basis) content is 35-45g/L, yeast extract 10g/L, glucose 20g/L, ammonium sulfate 0.5-0.7g/L, magnesium sulfate 0.3-0.4g/L, phosphoric acid dioxy potassium 1.8-2.3g/L; The agar powder of 15g/L is added during preparation solid medium.
Nutrient solution composition is: corn steep liquor (with dry basis) content is 35-45g/L; yeast extract 8-10g/L; glucose 18-22g/L; malt extract 25-30g, ammonium sulfate 0.5-0.7g/L, magnesium sulfate 0.3-0.4g/L; phosphoric acid dioxy potassium 1.8-2.3g/L; calcium carbonate 0.7g/L, ferrous sulfate 0.18g/L, manganous sulfate 0.025g/L; PH5.0.
Wet Penicillium cell preparation process is as follows: Penicillium notatum ATCC204052 through inclined-plane, shake-flask culture, obtain seed liquor.10L fermentor tank adds nutrient solution; Fermentor tank adds nutrient solution, 121 DEG C of autoclavings 30 minutes, be cooled to 23 DEG C, Penicillium notatum seed liquor is seeded to fermentor tank, inoculative proportion is 10-11%, and ventilation ratio is 0.9-1V/(V minute), cultivate 35-40 hour for 23 DEG C, wet Penicillium cell is obtained, as biocatalytic reaction catalyzer with filtering centrifuge is centrifugal.
Biocatalytic Conversion: add phosphate buffered saline buffer in 10L bottom ventilation stirred reactor, pH is 5.2, add substrate 3 '-fluoro acetophenone, its concentration is made to be 84g/L, corn steep liquor (with dry basis) content is 13g/L, sorbitan monolaurate 17.5g/L, sucrose 26g/L, maltose 34g/L, Virahol 5.5%V/V (volumetric concentration), 121 DEG C of autoclavings 30 minutes; When being cooled to 30-31 DEG C, add wet Penicillium cell and make its concentration be 66-70g/L, ventilation ratio is 0.11V/(V minute), carry out biocatalytic reaction, the reaction times is 67 hours; After reaction terminates, leach cell, be extracted with ethyl acetate reaction solution, steam ethyl acetate, obtain product (S)-3 '-fluorobenzene ethanol, reaction conversion ratio 98.5%, product yield 96.5%, enantiomeric excess rate 99%.
embodiment 4
Seed culture medium composition is: corn steep liquor (with dry basis) content is 35-45g/L, yeast extract 10g/L, glucose 20g/L, ammonium sulfate 0.5-0.7g/L, magnesium sulfate 0.3-0.4g/L, phosphoric acid dioxy potassium 1.8-2.3g/L; The agar powder of 15g/L is added during preparation solid medium.
Nutrient solution composition is: corn steep liquor (with dry basis) content is 35-45g/L; yeast extract 8-10g/L; glucose 18-22g/L; malt extract 25-30g, ammonium sulfate 0.5-0.7g/L, magnesium sulfate 0.3-0.4g/L; phosphoric acid dioxy potassium 1.8-2.3g/L; calcium carbonate 0.7g/L, ferrous sulfate 0.18g/L, manganous sulfate 0.025g/L; PH5.0.
Wet Penicillium cell preparation process is as follows: Penicillium notatum ATCC204052 through inclined-plane, shake-flask culture, obtain seed liquor.10L fermentor tank adds nutrient solution; Fermentor tank adds nutrient solution, 121 DEG C of autoclavings 30 minutes, be cooled to 24 DEG C, Penicillium notatum seed liquor is seeded to fermentor tank, inoculative proportion is 10-11%, and ventilation ratio is 0.9-1V/(V minute), cultivate 35-40 hour for 24 DEG C, wet Penicillium cell is obtained, as biocatalytic reaction catalyzer with filtering centrifuge is centrifugal.
Biocatalytic Conversion: add phosphate buffered saline buffer in 20L bottom ventilation stirred reactor, pH is 5.2, add substrate 3 '-fluoro acetophenone, its concentration is made to be 86g/L, corn steep liquor (with dry basis) content is 14g/L, sorbitan monolaurate 18g/L, sucrose 27g/L, maltose 35g/L, Virahol 6%V/V (volumetric concentration), 121 DEG C of autoclavings 30 minutes; When being cooled to 30 DEG C, add wet Penicillium cell and make its concentration be 69g/L, ventilation ratio is 0.11V/(V minute), carry out biocatalytic reaction, the reaction times is 68 hours; After reaction terminates, leach cell, be extracted with ethyl acetate reaction solution, steam ethyl acetate, obtain product (S)-3 '-fluorobenzene ethanol, reaction conversion ratio 99%, product yield 96.5%, enantiomeric excess rate 99%.
Claims (2)
1. the method for Penicillium cell biocatalysis preparation (S)-3 '-fluorobenzene ethanol, it is characterized in that adding phosphate buffered saline buffer in retort, pH is 5.2, add substrate 3 '-fluoro acetophenone, make substrate 3 '-fluoro acetophenone addition be 80-90g/L, corn steep liquor content is 12-15g/L, sorbitan monolaurate 17-18g/L, sucrose 25-28g/L, maltose 33-36g/L, Virahol 5-6%V/V, 121 DEG C of autoclavings 30 minutes; When being cooled to 30-31 DEG C, add wet Penicillium cell and make its concentration be 66-70g/L, ventilation ratio is 0.1-0.11V/(V minute), carry out biocatalytic reaction, the reaction times is 66-69 hour; After reaction terminates, leach cell, be extracted with ethyl acetate reaction solution, steam ethyl acetate, obtain product (S)-3 '-fluorobenzene ethanol, reaction conversion ratio 98-99%, product yield 96-97%, enantiomeric excess rate 98.5-99%.
2. method according to claim 1, is characterized in that wet Penicillium cell preparation process is as follows: Penicillium notatum ATCC204052, and through inclined-plane, shaking flask, seed tank culture obtain seed liquor; Fermentor tank adds nutrient solution, 121 DEG C of autoclavings 30 minutes, be cooled to 23-24 DEG C, Penicillium notatum seed liquor is seeded to fermentor tank, inoculative proportion is 10-11%, and ventilation ratio is 0.9-1V/(V minute), cultivate 35-40 hour for 23-24 DEG C, wet Penicillium cell is obtained, as biocatalytic reaction catalyzer with filtering centrifuge is centrifugal; In fermentor tank, nutrient solution composition is: corn steep liquor (with dry basis) content is 35-45g/L; yeast extract 8-10g/L; glucose 18-22g/L; malt extract 25-30g, ammonium sulfate 0.5-0.7g/L, magnesium sulfate 0.3-0.4g/L; phosphoric acid dioxy potassium 1.8-2.3g/L; calcium carbonate 0.7g/L, ferrous sulfate 0.18g/L, manganous sulfate 0.025g/L; PH5.0.
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