CN105080961A - Method for remedying soil contaminated by phenols through residues of mushroom media - Google Patents
Method for remedying soil contaminated by phenols through residues of mushroom media Download PDFInfo
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- CN105080961A CN105080961A CN201510386059.3A CN201510386059A CN105080961A CN 105080961 A CN105080961 A CN 105080961A CN 201510386059 A CN201510386059 A CN 201510386059A CN 105080961 A CN105080961 A CN 105080961A
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Abstract
The invention discloses a method for remedying soil contaminated by phenols through residues of mushroom media. The method particularly includes the steps that mushroom sticks are smashed to obtain mushroom residues; enzyme production inducing liquid is added to conduct cultivation under the suitable condition; after being obtained, a fermentation ripening system is added into the soil contaminated by phenolic compounds according to the proportion of 1:1 to 3:1; and dual functions of microbial remediation and phytoremediation can be achieved through degradation of the phenolic compounds by ligninase in a mushroom residue fermentation system and organic fertilizers provided by the system. Compared with other chemical remediation and physical remediation methods, the method is low in cost and free of secondary pollution, and the efficiency of the biology remediation method can be further improved.
Description
Technical field
Utilize a method for the biological prosthetic phenolic compounds pollution soil of edible fungus bran, be specifically related to add product enzyme inducer to edible fungus bran and phenolic compounds pollution soil is repaired.Belong to technical field of bioengineering.
Background technology
Phenolic compound refers to that the hydrogen atom in aromatic hydrocarbon on phenyl ring is optionally substituted by a hydroxyl group generated compound, and be the hydroxyl derivative of aromatic hydrocarbons, the hydroxy number contained by its molecule can be divided into monohydric phenol and polyhydric phenols.Phenol is a kind of chemical toxicant of moderate strength, with the protein generation chemical reaction in cell magma.Make cell degeneration during low concentration, during high concentration, make protein coagulating.Phenolic compound can enter in body through mucocutaneous, respiratory tract and alimentary canal.Low concentration can cause accumulative slow poisoning, and high concentration can cause acute poisoning so that death of going into a coma.Phenol in environment pollutes and mainly refers to that phenolic compound is to the pollution of water body, and phenol wastewater is one of industrial wastewater that in the world today, harm is large, pollution range is wide, is the important sources of water pollutions in environment.All containing phenol in the waste water that the industry such as many industrial circles such as coal gas, coking, oil refining, metallurgy, machine-building, glass, petrochemical industry, lumber fibre, chemical organic synthesis industry, plastics, medicine, agricultural chemicals, paint are discharged.If these waste water are without process, directly discharge, irrigated farmland then can atmosphere pollution, water, soil and food.
The reparation of current phenolic compounds pollution soil mainly comprises chemical means, physical means and biological means.Physical method is as extraction, absorption method, a ultrasonic hydrogen peroxide combination method, Wet Catalytic Oxidation Method, supercritical water oxidation method, high voltage pulse method etc.The above two are easy and simple to handle, but cost is higher, uneconomical in practical application; Rear several method belongs to current study hotspot, but processing cost is high, and treatment scale is little, is substantially also in the laboratory research stage.Bioremediation technology is a kind of emerging clean technologies grown up on biodegradable basis, and it is the development of traditional bioremediation.Compared with physics, chemical redemption contaminated soil technology, it is low that it has cost, do not destroy the soil environment required for plant growth, pollutant oxidative safety, non-secondary pollution, the features such as treatment effect is good, simple to operate.Biological prostheticly accelerating natural biology degradation rate by the optimization of environmental factor, is the clean technologies that a kind of efficient, economy and ecology can be born.At present, the bioremediation technology administering polycyclic aromatic hydrocarbon pollution mainly contains two classes: a class is microorganism remediation technology; Another kind of is phytoremediation.
Summary of the invention:
The object of the present invention is to provide a kind of method utilizing the biological prosthetic phenolic compounds pollution soil of edible fungus bran, the bacterium rod containing hypha of edible fungus is utilized to be raw material, add wherein and produce enzyme induction liquid promotion edible mushroom making full use of biomass material, and then obtain lignoenzyme and the bacterium chaff residue compared with low ratio of carbon to ammonium.By the fertilizer that lignoenzyme in bacterium chaff fermentation system provides the degraded of phenolic compound and system, the double action of microorganism remediation and phytoremediation can be realized.Greatly improve the efficiency of biological prosthetic means.
Bacterium rod refers to cotton seed hulls, wood chip, straw, corncob, bagasse and multiple kinds of crops stalk, industrial waste for primary raw material, is used for the bar-shaped solid mixt producing fruit body of edible fungi or fructification of gathering is later remaining after inoculation hypha of edible fungus cultivates a period of time.When hypha of edible fungus grows in the medium, need to produce a large amount of lignoenzyme to decompose lignocellulosic in matrix to obtain nutrition.Therefore, results or not yet gather in the crops fructification bacterium rod in containing a large amount of hypha of edible fungus.The concrete composition of bacterium rod can not as the restriction to reparation phenolic compounds pollution soil method of the present invention.
Industrial waste is mainly selected from vinegar grain, vinasse, paper mill effluent.
Edible mushroom is the artificial mushroom cultivated or can be under wild environment biology and eat.Lignoenzyme can be secreted to decompose lignocellulosic in matrix to obtain nutrition in its growth course.Described edible mushroom is mainly selected from mushroom (Agaricuscampestris), mushroom (Lentinulaedodes), agrocybe (Agrocybeaegerita), Hericium erinaceus (Hericiumerinaceum), phoenix-tail mushroom (Pleurotussajor-caju), agaricus bisporus (Agaricusbisporus), auricularia auriculajudae (Auriculariaauricula), Cordyceps sinensis (Cordycepssinensis), glossy ganoderma (Ganodermalucidum), pycnoporus samguineus (Pycnoporussanguineus), gold ear (Tremellaaurantialba), grey mushroom (abalonelike) (Pleurotusabalonus), flat mushroom (Pleurotusostreatus), asparagus (Flammulinavelutipes), pleurotus eryngii (Pleurotuseryngii), elegant precious mushroom (Pleurotusgeesteranus), coprinus comatus (Coprinuscomatus), Pleurotus nebrodensis (Pleurotusnebrodensis), lung shape picks up the ears (Pleurotuspulmonarius), Agricus blazei (Agaricusblazei) etc.
Technical scheme of the present invention:
The invention provides a kind of method utilizing the biological prosthetic phenolic compounds pollution soil of edible fungus bran, specifically comprise the steps: that the break process of bacterium rod obtains bacterium chaff; Add and produce enzyme induction liquid; Cultivate under suitable condition; By fermenting, the ratio of maturation system according to 1: 1-3: 1 joins in the soil by phenolic compounds pollution; Hyperaccumulative plant is planted in mixed system.
1. broken bacterium rod obtains bacterium chaff
After the bacterium of fructification of or not yet gathering rod removing polybag, utilize pulverizer that the fragmentation of bacterium rod is obtained uniform bacterium chaff.
2. add induction and produce enzyme liquid
The inducer that enzyme liquid contains lignoenzyme is produced in induction used, and other substances provide nitrogen element and trace element for hypha of edible fungus, can also adjust carbon-nitrogen ratio (C/N) and the pH value of system in addition, stimulates mycelia diauxic growth.Induction is produced enzyme liquid and is obtained by the prolonged and repeated test in this laboratory.
A kind of concrete embodiment, the usual addition that enzyme liquid is produced in every 10kg bacterium chaff induction is 10-50L, 10-30L can be selected further, the diauxic growth that more effectively can stimulate hypha of edible fungus in bacterium chaff when 12,13,15,16,17,19,20,23,25 or 28L is selected, the high expression of induction lignoenzyme and secretion when concrete.
3. cultivate under suitable condition
Suitable extraneous condition of culture can change the physiological status of hypha of edible fungus, better expression and secretion lignoenzyme, and then biomass material is fully degraded.
A concrete embodiment, cultivation temperature is generally 17-36 DEG C, can be chosen as 22-30 DEG C further, or is specifically chosen as 22,24,25,27,28,30 DEG C.The time of cultivating under above temperature conditions is 24-168 hour, or selects 24,72,96,120 or 144 hours further, can obtain that lignoenzyme content is higher, lignocellulosic transforms bacterium chaff more thoroughly.
4. the mixing of bacterium chaff fermentation system and contaminated soil
What obtain to step 3 obtains mixed system containing joining in contaminated soil with the ratio of 1: 1-3: 1 in the fermentation maturation system of a large amount of lignoenzyme.Utilize the lignoenzyme of higher concentration in fermentation maturation system to carry out biodegradation to the aldehydes matter in soil, stir weekly once.
5. carry out phytoremediation to mixed system inoculation hyperaccumulative plant
Obtain to step 4 plant that mixed system inoculation has super summation, utilize plant to repair the accumulation of pollutant and metabolic conversion.
Beneficial effect of the present invention:
The invention provides a kind of method utilizing the biological prosthetic phenolic compounds pollution soil of edible fungus bran.Be a kind of new way of bacterium chaff efficiency utilization, both can have reduced edible fungus bran and arbitrarily be discharged into the pollution problem that environment causes, and twice laid environmental protect can have been accomplished again, there is good application prospect.
Detailed description of the invention:
Below in conjunction with embodiment, the present invention is described further.Following examples are only a specific embodiment of the present invention, but design concept of the present invention is not limited thereto, and all changes utilizing this design the present invention to be carried out to unsubstantiality, all should belong to the behavior of invading scope.
Embodiment
Get 1kg flat mushroom bacterium rod, fragmentation adds the product enzyme induction liquid of 1.5L after obtaining bacterium chaff, cultivate 5 days at 23 DEG C, 2kg contaminated soil is added in fermentation maturation system, mix, stir weekly once, the trade route with better tolerance that in a month backward mixed system, inoculation 40 strain laboratory screenings obtain, plant and record biomass in soil after five months and significantly raise, phenolic compound concentration declines to a great extent.Demonstrate the repair that the method is good to phenolic compound.
Claims (6)
1. one kind utilizes the method for the biological prosthetic phenolic compounds pollution soil of edible fungus bran, be characterized in that the bacterium chaff to obtain after edible fungus stick fragmentation is raw material, add product enzyme inducer, fermentation maturation system is joined in the soil by phenolic compounds pollution, by the fertilizer that lignoenzyme in bacterium chaff fermentation system provides the degraded of phenolic compound and system, can realize the double action of microorganism remediation and phytoremediation, the method comprises the following steps:
A) by after the bacterium of fructification of or not yet gathering rod removing polybag, utilize pulverizer that the fragmentation of bacterium rod is obtained uniform bacterium chaff;
B) in obtained bacterium chaff, add product enzyme induction liquid according to the ratio of solid-liquid mass volume ratio 1: 1-4, after mixing, obtain fermentation system;
C) fermentation system is cultivated 24-168 hour under 17-36 DEG C of condition, carry out fermenting and producing lignocellulolyticenzymes;
D) the fermentation maturation system obtained joins in the soil by phenolic compounds pollution according to the ratio of 1: 1-3: 1.
2. utilize a method for the biological prosthetic phenolic compounds pollution soil of edible fungus bran, it is characterized in that described product enzyme induction formula of liquid is: in mass, urea is 0.1 ~ 5%, ammonium nitrate is 0.2 ~ 6%.
3. utilize a method for the biological prosthetic phenolic compounds pollution soil of edible fungus bran, it is characterized in that every 10kg bacterium rod needs the product enzyme induction liquid of 10L-40L.
4. utilize a method for the biological prosthetic phenolic compounds pollution soil of edible fungus bran, it is characterized in that cultivation temperature is 17-36 DEG C.
5. utilize a method for the biological prosthetic phenolic compounds pollution soil of edible fungus bran, it is characterized in that incubation time is 24-168 hour.
6. utilize a method for the biological prosthetic phenolic compounds pollution soil of edible fungus bran, it is characterized in that fermentation system and be 1: 1-3: 1 by the soil volume ratio of phenolic compounds pollution.
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| CN201510386059.3A CN105080961A (en) | 2015-07-01 | 2015-07-01 | Method for remedying soil contaminated by phenols through residues of mushroom media |
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| CN201510386059.3A CN105080961A (en) | 2015-07-01 | 2015-07-01 | Method for remedying soil contaminated by phenols through residues of mushroom media |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113058991A (en) * | 2021-01-20 | 2021-07-02 | 杨风 | In-situ remediation method for tobacco field organochlorine and organophosphorus pesticide composite contaminated soil |
Citations (4)
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| CN103008336A (en) * | 2012-12-28 | 2013-04-03 | 南京市环境保护科学研究院 | Ex-situ bioremediation method of soil contaminated by organic matters |
| CN103691736A (en) * | 2013-11-27 | 2014-04-02 | 无锡市金坤生物工程有限公司 | Remediation complex enzyme preparation for degrading soil PAH pollutant and soil remediation method by using the same |
| CN104098354A (en) * | 2014-07-31 | 2014-10-15 | 鸡西市食用菌科学研究所 | Method for converting fungus dregs into bio-organic fertilizer |
| CN104694513A (en) * | 2014-12-03 | 2015-06-10 | 江南大学 | Method for producing ligninase by using edible mushroom stick |
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2015
- 2015-07-01 CN CN201510386059.3A patent/CN105080961A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103008336A (en) * | 2012-12-28 | 2013-04-03 | 南京市环境保护科学研究院 | Ex-situ bioremediation method of soil contaminated by organic matters |
| CN103691736A (en) * | 2013-11-27 | 2014-04-02 | 无锡市金坤生物工程有限公司 | Remediation complex enzyme preparation for degrading soil PAH pollutant and soil remediation method by using the same |
| CN104098354A (en) * | 2014-07-31 | 2014-10-15 | 鸡西市食用菌科学研究所 | Method for converting fungus dregs into bio-organic fertilizer |
| CN104694513A (en) * | 2014-12-03 | 2015-06-10 | 江南大学 | Method for producing ligninase by using edible mushroom stick |
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| 刘魏魏等: "《生物表面活性剂强化微生物修复多环芳烃污染土壤的初探》", 《土壤学报》 * |
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| CN113058991A (en) * | 2021-01-20 | 2021-07-02 | 杨风 | In-situ remediation method for tobacco field organochlorine and organophosphorus pesticide composite contaminated soil |
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Application publication date: 20151125 |