CN105055015B - Irrecoverable electric perforating system - Google Patents
Irrecoverable electric perforating system Download PDFInfo
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- CN105055015B CN105055015B CN201510471661.7A CN201510471661A CN105055015B CN 105055015 B CN105055015 B CN 105055015B CN 201510471661 A CN201510471661 A CN 201510471661A CN 105055015 B CN105055015 B CN 105055015B
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Abstract
The invention discloses a kind of irrecoverable electric perforating system, including upper layer information administrative unit and high pressure pulse discharge major loop, upper layer information administrative unit occurs control unit, sequential function control unit, the first phototube coupling unit and power amplification circuit by high-frequency impulse and control signal is sent into high pressure pulse discharge major loop；Feedback signal is back to upper layer information administrative unit by high pressure pulse discharge major loop by voltage and current detection circuit, the second phototube coupling unit and signal filter unit.The present invention uses the high voltage pulse of nanosecond rising edge, carries out the electric shock perforation of nanometer scale to the outer membrane of biological cell, realizes the inactivating efficacy to biological cell.The system is applied in oncotherapy, the deactivation treatment to tumour cell can not only be realized, can also accomplish effect minimally invasive and without heat deposition, the damage to surrounding tissue is small, some can be prevented to be treated from the cancer for carrying out operative treatment using this device.
Description
Technical field
The present invention relates to one kind to treat tumour nano-knife, more particularly to a kind of irrecoverable electric perforating system.
Background technology
Tumour is a kind of common disease, frequently-occurring disease, and wherein malignant tumour is to endanger a kind of disease of human health most serious at present
Disease.Because the pathogenesis and the cause of disease of malignant tumour not yet understand completely, thus lack basic precautionary measures.So far,
The mankind there is no the image of Buddha to treat other common frdquently encountered diseases and cure malignant tumour like that.Due to intractable, the number century of malignant tumour
Since, scholars remove the method by performing the operation and chemicotherapy is combined, and malignant tumour are treated, to obtain longer life
Deposit the phase.But radiation and chemotherapy is very big on body immunity influence, life quality is had a strong impact on.
Since early stage in the 1970's, electroporation just be used to molecule being inserted into animal or plant intracellular.Usually,
Electroporation is a kind of technology for the macromolecules into cells for making to be unable to penetration cell film by electric pulse.If apply strong electricity
, then cell membrane is temporarily changed to porous, goes out permeability to external material exhibits.But up to the present, the method for electroporation is still
Cell-penetrating is only used for, is not applied further.
The content of the invention
The present invention is in order to solve above-mentioned problems of the prior art, there is provided it is a kind of can kill cell can not be extensive
Telegram in reply perforation system.
The present invention is adopted the technical scheme that to solve this problem：
A kind of irrecoverable electric perforating system, including upper layer information administrative unit and high pressure pulse discharge major loop, upper strata
By high-frequency impulse control unit, sequential function control unit, the first phototube coupling unit and power occur for information management unit
Control signal is sent to high pressure pulse discharge major loop by amplifying circuit；High pressure pulse discharge major loop passes through measure voltage ＆ current
Feedback signal is back to upper layer information administrative unit by circuit, the second phototube coupling unit and signal filter unit.Described is upper
Layer information management unit is medical computer.
Described high pressure pulse discharge major loop includes the energy capacitance set being connected with charge power supply and passes through swift electron
Switch the paired sparking electrode being connected with storage capacitor；Charge switch is provided between energy capacitance set and charge power supply, soon
Discharge switch is provided between fast electronic switch and sparking electrode.Energy capacitance set by 100uF ~ 400uF large bulk capacitance with
0.1uF ~ 1uF low capacity electric capacity composes in parallel.High-speed electronic switch uses insulated gate bipolar transistor, and is driven provided with IGBT
Dynamic circuit.
The present invention has the advantages and positive effects of：
The irrecoverable electric perforating system of the present invention, using the high voltage pulse of nanosecond rising edge, to biological cell
Outer membrane carries out the electric shock perforation of nanometer scale, realizes the inactivating efficacy to biological cell.The system is applied in oncotherapy,
The deactivation treatment to tumour cell can not only be realized, effect minimally invasive and without heat deposition can also be accomplished.Further, since can not
Inverse electroporation can only enter eleven punch 11 to general histocyte, and the phoirocyte such as blood vessel, nerve is then damaged under its effect
Wound is relatively light and can recover intact.Therefore, this high selectivity to therapeutic effect is treated, and the damage to surrounding tissue is small, can be with
Some are prevented to be treated from the cancer for carrying out operative treatment using this device.
Brief description of the drawings
Fig. 1 is the structured flowchart of the irrecoverable electric perforating system of the present invention；
Fig. 2 is the circuit theory diagrams of the high pressure pulse discharge major loop of the present invention.
Critical piece symbol description in accompanying drawing：
1：Sparking electrode 2：IGBT drive circuit.
Embodiment
The irrecoverable electric perforating system of the present invention is described in detail with specific embodiment referring to the drawings.Under
The specific embodiment of face description is only the preferred forms of the present invention, and is not considered as limiting the invention.
Fig. 1 is the structured flowchart of the irrecoverable electric perforating system of the present invention.As shown in figure 1, the present invention's is irrecoverable
Electric perforating system, including upper layer information administrative unit and high pressure pulse discharge major loop, upper layer information administrative unit pass through high frequency
Pulse generation control unit, sequential function control unit, the first phototube coupling unit and power amplification circuit pass control signal
Deliver to high pressure pulse discharge major loop；High pressure pulse discharge major loop passes through voltage and current detection circuit, the second Phototube Coupling list
Feedback signal is back to upper layer information administrative unit by member and signal filter unit.
High pressure pulse discharge major loop, for producing high-frequency pulse signal.Voltage and current detecting unit is used to detect high pressure
The voltage and current signal of the feedback signal point of pulsed discharge major loop.Signal filter unit is by high pressure pulse discharge major loop
The feedback signal of input is filtered processing.
Control unit occurs for high-frequency impulse, with field programmable gate array（FPGA or CPLD）It is real for central controller
Now to the control of high pressure pulse discharge major loop discharge and recharge.Control unit occurs for high-frequency impulse and sequential function control unit is common
Realize that the connection control to electrode, monitoring and data acquisition to charging voltage are right to charging interval, the control of discharging time
The setting of the parameters such as the width of discharge pulse, pulse number, the monitoring to discharge process, the data acquisition of discharge voltage, electric current
Deng control and monitoring function.Power amplification circuit carries out power amplification for the control signal of output.
Two phototube coupling units are that all input/output signals do Phototube Coupling, internal to reduce external noise signals
The noise jamming of portion's control unit, improve the reliability of control unit.
Upper layer information administrative unit is medical computer.Upper layer information administrative unit is that operator sets various running parameters
The approach of interaction is provided, and the running parameter set is descended into function control unit；Receive function control unit collection
Various data, data are handled and Drawing of Curve and shown, the letter of charging and discharging process is provided for operator
Breath；Programmed environment is provided for operator, realizes the intelligentized control method to system discharge order.
Fig. 2 is the circuit theory diagrams of the high pressure pulse discharge major loop of the present invention.As shown in Fig. 2 high pressure pulse discharge master
Loop include the energy capacitance set that be connected with charge power supply and by high-speed electronic switch K1 be connected with storage capacitor in pairs
Sparking electrode 1.Charge switch KJ1 and KJ2, high-speed electronic switch and electric discharge electricity are provided between energy capacitance set and charge power supply
Discharge switch KJ8 and KJ5 are provided between pole.Energy capacitance set by one or more 100uF ~ 400uF large bulk capacitance with
One or more 0.1uF ~ 1uF low capacity electric capacity composes in parallel.Energy capacitance set can supply high current in a short time, real
The rapid increase of existing current impulse.There are two large bulk capacitance C1 and C2 series connection, two low capacity electric capacity C3 and C4 series connection in figure
Afterwards, then row composes in parallel energy capacitance set.High-speed electronic switch uses insulated gate bipolar transistor, and provided with IGBT driving electricity
Road 2.
After two resistance R1 and R2 series connection, one end is connected between two charge switch KJ1 and KJ2, other end ground connection, resistance
First feedback signal point is set between R1 and R2.After two resistance R3 and R4 series connection, one end be connected to high-speed electronic switch K1 with
Between KJ8, other end ground connection, and be connected by R5 with KJ5, the second feedback signal point is set between resistance R3 and R4.System work
When making, sparking electrode is inserted into lesions position, closes KJ1 and KJ2 first, is charged for energy capacitance set.When the voltage of the point reaches
During to predetermined value, KJ1 and KJ2 is disconnected, closes KJ8 and KJ5, energy capacitance set electric discharge, high-speed electronic switch drives electricity in IGBT
Under the effect of road, the pulse of high-frequency high-voltage is formed, the width of pulse can be set as 100us.In the rising edge of pulse, realization pair
The perforation of cell, the process of the perforation is irreversible, it is achieved thereby that cell inactivation.
The irrecoverable electric perforating system of the present invention, using the high voltage pulse of nanosecond rising edge, to biological cell
Outer membrane carries out the electric shock perforation of nanometer scale, realizes the inactivating efficacy to biological cell.The system is applied in oncotherapy,
The deactivation treatment to tumour cell can not only be realized, effect minimally invasive and without heat deposition can also be accomplished.Further, since can not
Inverse electroporation can only enter eleven punch 11 to general histocyte, and the phoirocytes such as blood vessel, nerve are not worked, therefore, this
Kind the high selectivity of therapeutic effect is treated, the damage to surrounding tissue is small, can prevent some from the cancer that carries out operative treatment
Disease, it can be treated using this device.The present invention uses the form of capacitance group discharge and recharge, with field programmable gate array
(FPGA) it is central controller, is steep-sided pulse generation switch with igbt (IGBT), realizes output pulse
Rise time is less than 62ns, frequency-adjustable：1Hz-100kHz, pulse amplitude are adjustable：0-3500V, pulse width are adjustable：30-100
μ s, output umber of pulse are arbitrarily set.Stimulated, realized by applying steep-sided pulse to the breast cancer tumor cells in sterile white mouse body
The inactivation of tumour cell, test result indicates that：The present invention can be effectively applied to the correlation of the lower oncotherapy of steep-sided pulse effect
Research；It will be can apply to after commercialization in the treatment of kinds cancer illness.
Claims (4)
1. a kind of irrecoverable electric perforating system, the system includes upper layer information administrative unit and high pressure pulse discharge major loop,
Upper layer information administrative unit by high-frequency impulse occur control unit, sequential function control unit, the first phototube coupling unit and
Control signal is sent to high pressure pulse discharge major loop by power amplification circuit；The high pressure pulse discharge major loop is included with filling
The energy capacitance set of power supply connection and the paired sparking electrode being connected by high-speed electronic switch with energy capacitance set；It is special
Sign is, is provided with two charge switch between the energy capacitance set and charge power supply, and one end after two resistant series connects
It is connected between two charge switch, other end ground connection；Divide between the high-speed electronic switch and two sparking electrodes
Be not provided with a discharge switch, one end after two resistant series be connected to the high-speed electronic switch with described in one of them
Between discharge switch, other end ground connection, and be connected by resistance with discharge switch another described；The high pressure pulse discharge master
Feedback signal is back to upper strata by voltage and current detection circuit, the second phototube coupling unit and signal filter unit and believed by loop
Cease administrative unit.
2. irrecoverable electric perforating system according to claim 1, it is characterised in that：Upper layer information administrative unit is medical
Computer.
3. irrecoverable electric perforating system according to claim 1, it is characterised in that：Energy capacitance set by 100uF~
The low capacity electric capacity of 400uF large bulk capacitance and 0.1uF~1uF composes in parallel.
4. irrecoverable electric perforating system according to claim 1, it is characterised in that：High-speed electronic switch uses insulated gate
Bipolar transistor, and it is provided with IGBT drive circuit.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510471661.7A CN105055015B (en) | 2015-08-05 | 2015-08-05 | Irrecoverable electric perforating system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201510471661.7A CN105055015B (en) | 2015-08-05 | 2015-08-05 | Irrecoverable electric perforating system |
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| CN105055015A CN105055015A (en) | 2015-11-18 |
| CN105055015B true CN105055015B (en) | 2018-03-13 |
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Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108780085A (en) * | 2016-01-15 | 2018-11-09 | 辛辛那提大学 | Advanced electroporation device and method for obtaining the analyte in biofluid |
| CN106939298B (en) * | 2017-03-20 | 2019-06-25 | 中国人民解放军第三军医大学 | A kind of full tumour antigen preparation method of glioma and device |
| CN108186108A (en) * | 2017-10-12 | 2018-06-22 | 天津市鹰泰利安康医疗科技有限责任公司 | Multipole bidirectional high-pressure steep-sided pulse discharge system and charging method |
| CN108853719A (en) * | 2018-04-26 | 2018-11-23 | 费兴波 | A kind of high voltage narrow pulse treatment system |
| CN111374750B (en) * | 2019-08-06 | 2021-11-02 | 深圳钮迈科技有限公司 | Pulse real-time monitoring circuit and tumor therapeutic instrument |
| WO2021114219A1 (en) * | 2019-12-13 | 2021-06-17 | 天津市鹰泰利安康医疗科技有限责任公司 | High-frequency bipolar unrecoverable electroporation system |
| CN110946642A (en) * | 2019-12-13 | 2020-04-03 | 天津市鹰泰利安康医疗科技有限责任公司 | High-frequency bipolar unrecoverable electroporation system |
| CN114391943B (en) * | 2022-01-25 | 2022-10-11 | 天津市鹰泰利安康医疗科技有限责任公司 | Treatment equipment based on multi-electrode bidirectional pulse discharge |
| CN114601556B (en) * | 2022-04-19 | 2022-09-20 | 天津市鹰泰利安康医疗科技有限责任公司 | Electrode for pulsed field ablation stimulation, use method and voltage regulation method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN2865739Y (en) * | 2005-11-04 | 2007-02-07 | 孙辉 | Multifunction square wave electronic punching instrument |
| CN100469331C (en) * | 2007-03-13 | 2009-03-18 | 重庆大学 | Tumor treating steep pulse equipment and process |
| CN101085391B (en) * | 2007-06-29 | 2010-05-26 | 重庆大学 | Device for inducing tumor cell apoptosis by high-voltage nanosecond pulse |
| CN101342390A (en) * | 2007-07-10 | 2009-01-14 | 上海塔瑞莎健康科技有限公司 | Electroporation leading-in apparatus for medicament |
| CN101972168B (en) * | 2010-11-16 | 2012-05-02 | 重庆大学 | Device for treating tumors through irreversible electroporation |
| CN202458567U (en) * | 2012-01-18 | 2012-10-03 | 姚合良 | Convenient operating forceps |
| CN204971570U (en) * | 2015-08-05 | 2016-01-20 | 天津市鹰泰利安康医疗科技有限责任公司 | Unrecoverable electroporation system |
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Inventor after: Xue Zhixiao Inventor after: Zhang Xiaochen Inventor after: Zhang Jianhao Inventor after: Li Honggang Inventor before: Gu Weiyi Inventor before: Xue Zhixiao Inventor before: Zhang Lei Inventor before: Ren Haixing Inventor before: Li Honggang |
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Effective date of registration: 20220330 Address after: 201804 room 1002-3, room 1002-5 and room 1006-2, floor 10, No. 1112 Hanggui Road, Anting Town, Jiading District, Shanghai Patentee after: Shanghai Yuanshan Medical Technology Co.,Ltd. Address before: 300384 - 2-2-208, No. 11, Haitai development Fourth Road, Huayuan Industrial Zone (outside the ring), Xiqing District, Tianjin Patentee before: TIANJIN INTELLIGENT HEALTH Co.,Ltd. |